TY - JOUR A1 - Savitskaya, Irina A1 - Zhantlessova, Sirina A1 - Kistaubayeva, Aida A1 - Ignatova, Ludmila A1 - Shokatayeva, Dina A1 - Sinyavsky, Yuriy A1 - Kushugulova, Almagul A1 - Digel, Ilya T1 - Prebiotic cellulose–pullulan matrix as a “vehicle” for probiotic biofilm delivery to the host large intestine JF - Polymers N2 - This study describes the development of a new combined polysaccharide-matrix-based technology for the immobilization of Lactobacillus rhamnosus GG (LGG) bacteria in biofilm form. The new composition allows for delivering the bacteria to the digestive tract in a manner that improves their robustness compared with planktonic cells and released biofilm cells. Granules consisting of a polysaccharide matrix with probiotic biofilms (PMPB) with high cell density (>9 log CFU/g) were obtained by immobilization in the optimized nutrient medium. Successful probiotic loading was confirmed by fluorescence microscopy and scanning electron microscopy. The developed prebiotic polysaccharide matrix significantly enhanced LGG viability under acidic (pH 2.0) and bile salt (0.3%) stress conditions. Enzymatic extract of feces, mimicking colon fluid in terms of cellulase activity, was used to evaluate the intestinal release of probiotics. PMPB granules showed the ability to gradually release a large number of viable LGG cells in the model colon fluid. In vivo, the oral administration of PMPB granules in rats resulted in the successful release of probiotics in the colon environment. The biofilm-forming incubation method of immobilization on a complex polysaccharide matrix tested in this study has shown high efficacy and promising potential for the development of innovative biotechnologies. KW - immobilization KW - prebiotic KW - bacterial cellulose KW - biofilms KW - Lactobacillus rhamnosus GG Y1 - 2023 U6 - http://dx.doi.org/10.3390/polym16010030 N1 - This article belongs to the Section "Polymer Composites and Nanocomposites" IS - 16(1) PB - MDPI CY - Basel ER - TY - JOUR A1 - Digel, Ilya A1 - Akimbekov, Nuraly A1 - Rogachev, Evgeniy A1 - Pogorelova, Natalia T1 - Bacterial cellulose produced by Medusomyces gisevii on glucose and sucrose: biosynthesis and structural properties JF - Cellulose N2 - In this work, the effects of carbon sources and culture media on the production and structural properties of bacterial cellulose (BC) synthesized by Medusomyces gisevii have been studied. The culture medium was composed of different initial concentrations of glucose or sucrose dissolved in 0.4% extract of plain green tea. Parameters of the culture media (titratable acidity, substrate conversion degree etc.) were monitored daily for 20 days of cultivation. The BC pellicles produced on different carbon sources were characterized in terms of biomass yield, crystallinity and morphology by field emission scanning electron microscopy (FE-SEM), atomic force microscopy and X-ray diffraction. Our results showed that Medusomyces gisevii had higher BC yields in media with sugar concentrations close to 10 g L−1 after a 18–20 days incubation period. Glucose in general lead to a higher BC yield (173 g L−1) compared to sucrose (163.5 g L−1). The BC crystallinity degree and surface roughness were higher in the samples synthetized from sucrose. Obtained FE-SEM micrographs show that the BC pellicles synthesized in the sucrose media contained densely packed tangles of cellulose fibrils whereas the BC produced in the glucose media displayed rather linear geometry of the BC fibrils without noticeable aggregates. KW - Bacterial cellulose KW - Medusomyces gisevi KW - Carbon sources KW - Culture media KW - Cellulose nanostructure Y1 - 2023 U6 - http://dx.doi.org/10.1007/s10570-023-05592-z SN - 1572-882X (Online) SN - 0969-0239 (Print) N1 - Corresponding author: Ilya Digel PB - Springer Science + Business Media CY - Dordrecht ER - TY - JOUR A1 - Turaliyeva, M. A1 - Yeshibaev, A. A1 - Saparbekova, A. A1 - Akynova, L. A1 - Abildayeva, R. A1 - Sadenova, M. A1 - Sartayeva, K. A1 - Schieffer, Andre A1 - Digel, Ilya T1 - Species composition and injuriousness of stranger xylophilous fauna affecting indigenous urban dendroflora of Central Asia JF - Asian journal of microbiology, biotechnology & environmental sciences : AJMBES N2 - At the present time, one of the most serious environmental problems of Central Asia and South Kazakhstan is the ongoing large-scale deterioration of principal urban tree populations. Several major centers of massive spread of invasive plant pests have been found in urban dendroflora of this region. The degree of damage of seven most wide-spread aboriginal tree species was found to range from 21.4±1.1 to 85.4±1.8%. In particular, the integrity of the native communities of sycamore (Platanus spp.), willow (Salix spp.), poplar (Populus spp.) and elm (Ulmus spp.) is highly endangered. Our taxonomic analysis of the most dangerous tree pests of the region has revealed them as neobiontic xylophilous insects such as Cossus cossus L. (Order: Lepidoptera L.) Monochamus urussovi Fisch., Monochamus sutor L., Acanthocinus aedelis L. and Ñetonia aureate L. (Order: Coleoptera L.). We relate the origin of this threatening trend with the import of industrial wood in the mid 90’s of the last century that was associated with high degree of the constructional work in the region. Because of the absence of efficient natural predators of the pest species, the application of microbiological methods of the pest control and limitation is suggested. Y1 - 2016 SN - 0972-3005 VL - 18 IS - 2 SP - 359 EP - 366 PB - EM International ER - TY - JOUR A1 - Digel, Ilya T1 - In-situ biological decontamination of an ice melting probe Y1 - 2010 N1 - 38th COSPAR Scientific Assembly. Held 18-15 July 2010, in Bremen, Germany Abstract unter https://www.cospar-assembly.org/abstractcd/OLD/COSPAR-10/abstracts/data/pdf/abstracts/F36-0013-10.pdf ER - TY - JOUR A1 - Blome, Hans-Joachim A1 - Seboldt, Wolfgang A1 - Dachwald, Bernd A1 - Richter, Lutz T1 - Proposal for an integrated European Space Exploration Study JF - Space Debris and Space Traffic Management Symposium 2004 : proceedings of the International Academy of Astronautics Space Debris and Space Traffic Management Symposium, held in conjunction with the 55th International Astronautical Congress (IAC), October 4 - 8, 2004, Vancouver, British Columbia, Canada / ed. by Joerg Bendisch Y1 - 2004 SN - 0-87703-523-7 N1 - Proceedings IAC-2004-IAA, 3.6.1.06; Space Debris and Space Traffic Management Symposium <2004, Vancouver, British Columbia> ; International Academy of Astronautics PB - Univelt CY - San Diego, Calif. ER - TY - JOUR A1 - Dachwald, Bernd A1 - Seboldt, W. A1 - Loeb, H. W A1 - Schartner, K.-H. T1 - Main Belt Asteroid Sample Return Mission Using Solar Electric Propulsion JF - Acta Astronautica. 63 (2008), H. 1-4 Y1 - 2008 SN - 0094-5765 N1 - International Astronautical Federation Congress <58, 2007, Hyderabad> ; International Astronautical Congress <58, 2007, Hyderabad> ; IAC-07-A3.5.07 SP - 91 EP - 101 ER - TY - JOUR A1 - Dachwald, Bernd A1 - Ohndorf, A. T1 - 1st ACT Global Trajectory Optimisation Competition : Results found at DLR JF - Acta Astronautica. 61 (2007), H. 9 Y1 - 2007 SN - 0094-5765 N1 - Global Trajectory Optimization ; Results of the First Competition Organised by the Advanced Concept Team (ACT) of the European Space Agency (ESA) SP - 742 EP - 752 ER - TY - JOUR A1 - Dachwald, Bernd A1 - Seboldt, W. A1 - Richter, L. T1 - Multiple rendezvous and sample return missions to near-Earth objects using solar sailcraft / Dachwald, B. ; Seboldt, W. ; Richter, L. JF - Acta Astronautica. 59 (2006), H. 8-11 Y1 - 2006 SN - 0094-5765 N1 - International Conference on Low Cost Planetary Missions <5, 2003, Noordwijk> ; Selected Proceedings SP - 768 EP - 776 ER - TY - JOUR A1 - Dachwald, Bernd A1 - Seboldt, W. T1 - Multiple Near-Earth Asteroid Rendezvous and Sample Return Using First Generation Solar Sailcraft JF - Acta Astronautica. 57 (2005), H. 11 Y1 - 2005 SN - 0094-5765 SP - 864 EP - 875 ER - TY - JOUR A1 - Dachwald, Bernd T1 - Optimization of very-low-thrust trajectories using evolutionary neurocontrol JF - Acta Astronautica. 57 (2005), H. 2-8 Y1 - 2005 SN - 0094-5765 SP - 175 EP - 185 ER - TY - JOUR A1 - Dachwald, Bernd A1 - McDonald, Malcolm A1 - McInnes, Colin R. A1 - Mengali, Giovanni T1 - Impact of Optical Degradation on Solar Sail Mission Performance JF - Journal of Spacecraft and Rockets. 44 (2007), H. 4 Y1 - 2007 SN - 0022-4650 N1 - 2. ISSN: 1533-6794 SP - 740 EP - 749 ER - TY - JOUR A1 - Dachwald, Bernd A1 - Wi, Bong T1 - Solar Sail Kinetic Energy Impactor Trajectory Optimization for an Asteroid-Deflection Mission JF - Journal of Spacecraft and Rockets. 44 (2007), H. 4 Y1 - 2007 SN - 0022-4650 N1 - 2. ISSN: 1533-6794 SP - 755 EP - 764 ER - TY - JOUR A1 - Dachwald, Bernd T1 - Minimum Transfer Times for Nonperfectly Reflecting Solar Sailcraft JF - Journal of Spacecraft and Rockets. 41 (2004), H. 4 Y1 - 2004 SN - 0022-4650 N1 - 2. ISSN: 1533-6794 SP - 693 EP - 695 ER - TY - JOUR A1 - Dachwald, Bernd A1 - Mengali, Giovanni A1 - Quarta, Alessandrao A. A1 - Macdonald, Malcolm T1 - Parametric Model and Optimal Control of Solar Sails with Optical Degradation JF - Journal of guidance, control, and dynamics. 29 (2006), H. 5 Y1 - 2006 SN - 0162-3192 N1 - 2. ISSN: 0731-5090 SP - 1170 EP - 1178 ER - TY - JOUR A1 - Dachwald, Bernd T1 - Optimal Solar Sail Trajectories for Missions to the Outer Solar System JF - Journal of guidance, control, and dynamics. 28 (2005), H. 6 Y1 - 2005 SN - 0162-3192 N1 - 2. ISSN: 0162-3192. - 3. ISSN: 0731-5090 SP - 1187 EP - 1193 ER - TY - JOUR A1 - Dachwald, Bernd T1 - Optimization of Interplanetary Solar Sailcraft Trajectories Using Evolutionary Neurocontrol JF - Journal of guidance, control, and dynamics. 27 (2004), H. 1 Y1 - 2004 SN - 0162-3192 N1 - 2. ISSN: 0162-3192. - 3. ISSN: 0731-5090 SP - 66 EP - 72 ER - TY - JOUR A1 - Dachwald, Bernd A1 - Kahle, Ralph A1 - Wie, Bong T1 - Solar Sailing Kinetic Energy Impactor (KEI) Mission Design Tradeoffs for Impacting and Deflecting Asteroid 99942 Apophis JF - AIAA Guidance, Navigation, and Control Conference & Exhibit - AIAA Atmospheric Flight Mechanics Conference & Exhibit - AIAA Modeling and Simulation Technologies Conference & Exhibit - AIAA/AAS Astrodynamics Specialist Conference & Exhibit : [21 - 24 August 2006, Keystone, Colorado ; papers]. - (AIAA meeting papers on disc ; [11.]2006,19-20 ) Y1 - 2006 SN - 1-56347-802-1 N1 - American Institute of Aeronautics and Astronautics ; American Astronautical Society ; AIAA/AAS Astrodynamics Specialist Conference & Exhibit <2006, Keystone, Colo.> ; AIAA paper number: AIAA-2006-6178 SP - 1 EP - 20 PB - American Institute of Aeronautics and Astronautics CY - Reston, Va. ER - TY - JOUR A1 - Dachwald, Bernd A1 - Ohndorf, A. A1 - Wie, Bong T1 - Solar Sail Trajectory Optimization for the Solar Polar Imager (SPI) Mission JF - AIAA Guidance, Navigation, and Control Conference & Exhibit - AIAA Atmospheric Flight Mechanics Conference & Exhibit - AIAA Modeling and Simulation Technologies Conference & Exhibit - AIAA/AAS Astrodynamics Specialist Conference & Exhibit : [21 - 24 August 2006, Keystone, Colorado ; papers]. - (AIAA meeting papers on disc ; [11.]2006,19-20 ) Y1 - 2006 SN - 1-56347-802-1 N1 - American Institute of Aeronautics and Astronautics ; American Astronautical Society ; AIAA/AAS Astrodynamics Specialist Conference & Exhibit <2006, Keystone, Colo.> ; AIAA paper number: AIAA-2006-6177 PB - American Institute of Aeronautics and Astronautics CY - Reston, Va. ER - TY - JOUR A1 - Dachwald, Bernd A1 - Seboldt, Wolfgang A1 - Macdonald, Malcolm A1 - Mengali, Giovanni A1 - Quatra, Alessandro A. A1 - McInnes, Colin R. A1 - Rios-Reyes, Leonel A1 - Scheerers, Daniel J. A1 - Wie, Bong A1 - Görlich, Marianne A1 - Lura, Franz A1 - Diedrich, Benjamin A1 - Baturkin, Volodymyr A1 - Coverstone, Victoria L. A1 - Leipold, Manfred A1 - Garbe, Gregory P. T1 - Potential Solar Sail Degradation Effects on Trajectory and Attitude Control JF - AIAA Guidance, Navigation and Control Conference and Exhibit - AIAA Modeling and Simulation Technologies Conference and Exhibit - AIAA Atmospheric Flight Mechanics Conference and Exhibit : [San Francisco, California, 15 - 18 August 2005 ; papers]. - (AIAA meeting papers on disc ; [10.]2005,16-17) Y1 - 2005 SN - 1-56347-765-3 N1 - American Institute of Aeronautics and Astronautics ; AIAA Guidance, Navigation, and Control Conference and Exhibit <2005, San Francisco, Calif.> ; AIAA paper number: AIAA-2006-6172 Link "https://arc.aiaa.org/doi/book/10.2514/MGNC05" am 15.07.2022 nachgetragen Behr PB - American Institute of Aeronautics and Astronautics CY - Reston, Va. ER - TY - JOUR A1 - Dachwald, Bernd A1 - Seboldt, W. A1 - Richter, L. T1 - Multiple Rendezvous and Sample Return Missions to Near-Earth Asteroids Using Solar Sailcraft JF - Proceedings of the Fifth IAA International Conference on Low Cost Planetary Missions : 24 - 26 September 2003, ESTEC, Noordwijk, the Netherlands / [comp. by R. A. Harris] Y1 - 2003 SN - 92-9092-853-0 N1 - International Conference on Low Cost Planetary Missions <5, 2003, Noordwijk> ; International Academy of Astronautics ; European Space Research and Technology Centre SP - 351 EP - 358 PB - ESA CY - Noordwijk ER -