TY  - JOUR
A1  - Artmann, Gerhard
A1  - Schmid-Schönbein, H.
A1  - Grebe, R.
A1  - Teitel, P. [u.a.]
T1  - Restoration of Microsieve Filterability of Human Red Cells After Exposure to Hyperosmolarity and Lactazidosis: Effect of Vinpocetine. Schmid-Schönbein, H.; Grebe, R.; Teitel, P.; Artmann, Gerhard Michael, Eschweiler, H.; Schröder, Susanne
JF  - Drug Development Research. 14 (1988), H. 3-4
Y1  - 1988
SN  - 1098-2299
N1  - Abstracts frei unter:
SP  - 205
EP  - 211
ER  - 
TY  - JOUR
A1  - Kurulgan Demirci, Eylem
A1  - Demirci, Taylan
A1  - Linder, Peter
A1  - Trzewik, Jürgen
A1  - Gierkowski, Jessica Ricarda
A1  - Gossmann, Matthias
A1  - Kayser, Peter
A1  - Porst, Dariusz
A1  - Digel, Ilya
A1  - Artmann, Gerhard
A1  - Temiz Artmann, Aysegül
T1  - rhAPC reduces the endothelial cell permeability via a decrease of contractile tensions induced by endothelial cells
JF  - Journal of Bioscience and Bioengineering
N2  - All cells generate contractile tension. This strain is crucial for mechanically controlling the cell shape, function and survival. In this study, the CellDrum technology quantifying cell's (the cellular) mechanical tension on a pico-scale was used to investigate the effect of lipopolysaccharide (LPS) on human aortic endothelial cell (HAoEC) tension. The LPS effect during gram-negative sepsis on endothelial cells is cell contraction causing endothelium permeability increase. The aim was to finding out whether recombinant activated protein C (rhAPC) would reverse the endothelial cell response in an in-vitro sepsis model. In this study, the established in-vitro sepsis model was confirmed by interleukin 6 (IL-6) levels at the proteomic and genomic levels by ELISA, real time-PCR and reactive oxygen species (ROS) activation by florescence staining. The thrombin cellular contraction effect on endothelial cells was used as a positive control when the CellDrum technology was applied. Additionally, the Ras homolog gene family, member A (RhoA) mRNA expression level was checked by real time-PCR to support contractile tension results. According to contractile tension results, the mechanical predominance of actin stress fibers was a reason of the increased endothelial contractile tension leading to enhanced endothelium contractility and thus permeability enhancement. The originality of this data supports firstly the basic measurement principles of the CellDrum technology and secondly that rhAPC has a beneficial effect on sepsis influenced cellular tension. The technology presented here is promising for future high-throughput cellular tension analysis that will help identify pathological contractile tension responses of cells and prove further cell in-vitro models.
KW  - Cell permeability
KW  - Cellular force
KW  - Endothelial cells
KW  - Recombinant activated protein C
KW  - Lipopolysaccharide
KW  - Contractile tension
KW  - CellDrum
Y1  - 2012
U6  - http://dx.doi.org/10.1016/j.jbiosc.2012.03.019
SN  - 1347-4421
VL  - 113
IS  - 2
SP  - 212
EP  - 219
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - BOOK
A1  - Artmann, Gerhard
T1  - Stem cell engineering : principles and applications / Gerhard M. Artmann ... eds.
Y1  - 2011
SN  - 978-3-642-11864-7
PB  - Springer
CY  - Berlin [u.a.]
ER  - 
TY  - JOUR
A1  - Zerlin, Kay
A1  - Kasischke, Nicole
A1  - Digel, Ilya
A1  - Maggakis-Kelemen, Christina
A1  - Temiz Artmann, Aysegül
A1  - Porst, Dariusz
A1  - Kayser, Peter
A1  - Linder, Peter
A1  - Artmann, Gerhard
T1  - Structural transition temperature of hemoglobins correlates with species’ body temperature
JF  - European Biophysics Journal. 37 (2007), H. 1
Y1  - 2007
SN  - 1432-1017
SP  - 1
EP  - 10
ER  - 
TY  - JOUR
A1  - Artmann, Gerhard
A1  - Keller, U.
A1  - Haas, J.
A1  - Amort, U.
A1  - Zang, Werner
A1  - Karschies, S.
T1  - Technik und ausgewählte Applikationen eines Membranbioassays roter Blutzellen
JF  - Biomedizinische Technik = Biomedical engineering. 36 (1991)
Y1  - 1991
SN  - 0013-5585
VL  - 36
IS  - S1
SP  - 450
EP  - 451
ER  - 
TY  - JOUR
A1  - Artmann, Gerhard
A1  - Stache, O.
A1  - Königsfeld, J.
T1  - Temperaturabhängigkeit der Pseudopodienbildung und Rückbildung von Granulozyten / Stache, O. ; Königsfeld, J. ; Artmann, G. M.
JF  - Biomedizinische Technik = Biomedical Engineering. 45 (2000), H. s1
Y1  - 2000
SN  - 1862-278X
SP  - 450
EP  - 451
ER  - 
TY  - JOUR
A1  - Artmann, Gerhard
A1  - Kelemen, C.
A1  - Chien, S.
T1  - Temperature transition of human hemoglobin at body temperature: effects of calcium. Kelemen, C.; Chien, S.; Artmann, Gerhard Michael
JF  - Biophysical journal. 80 (2001), H. 6
Y1  - 2001
SN  - 1542-0086
SP  - 2622
EP  - 2630
ER  - 
TY  - JOUR
A1  - Artmann, Gerhard
A1  - Kelemen, Christina
A1  - Porst, Dariusz
A1  - Büldt, G. [u.a.]
T1  - Temperature transitions of protein properties in human red blood cells. Artmann, Gerhard Michael, Kelemen, Christina; Porst, D.; Büldt, G.; Chien, S.
JF  - Biophysical Journal. 75 (1998), H. 6
Y1  - 1998
SN  - 1542-0086
N1  - http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=1299989&blobtype=pdf
SP  - 3179
EP  - 3183
ER  - 
TY  - JOUR
A1  - Artmann, Gerhard
A1  - Schmid-Schönbein, H.
T1  - The microscope-photometrical-monolayer-technique- a new principle for analyzing the distribution of rheological responses in pathological and experimentally modified red cell populations. Artmann, Gerhard Michael; Schmid-Schönbein, H.
JF  - Microcirculation, an update : proceedings of the Fourth World Congress for Microcirculation, Tokyo, Japan, July 26-30, 1987
Y1  - 1987
SN  - 0444809511
N1  - World Congress for Microcirculation (4, 1987, Tokyo;); International congress series ; 755
SP  - 475
EP  - 477
PB  - Excerpta Medica
CY  - Amsterdam [u.a.]
ER  - 
TY  - JOUR
A1  - Digel, Ilya
A1  - Zhubanova, Azhar A.
A1  - Nojima, H.
A1  - Artmann, Gerhard
T1  - The use of bactericidal effects of cluster ions generated by plasma in medical biotechnology
JF  - Biotechnologija : teorija i praktika (2004)
Y1  - 2004
SN  - 1028-9399
SP  - 46
EP  - 52
ER  -