TY  - JOUR
A1  - Immel, Timo
A1  - Grützke, Martin
A1  - Späte, Anne-Katrin
A1  - Groth, Ulrich
A1  - Öhlschläger, Peter
A1  - Huhn, Thomas
T1  - Synthesis and X-ray structure analysis of a heptacoordinate titanium(IV)-bis-chelate with enhanced in vivo antitumor efficacy
JF  - Chemical Communications
N2  - Chelate stabilization of a titanium(IV)–salan alkoxide by ligand exchange with 2,6-pyridinedicarboxylic acid (dipic) resulted in heptacoordinate complex 3 which is not redox-active, stable on silica gel and has increased aqueous stability. 3 is highly toxic in HeLa S3 and Hep G2 and has enhanced antitumor efficacy in a mouse cervical-cancer model.
Y1  - 2012
U6  - https://doi.org/10.1039/C2CC31624B
SN  - 1364-548X
VL  - 48
IS  - 46
SP  - 5790
EP  - 5792
PB  - Royal Society of Chemistry
CY  - Cambridge
ER  - 
TY  - JOUR
A1  - Henken, F. E.
A1  - Oosterhuis, K.
A1  - Öhlschläger, Peter
A1  - Bosch, L.
A1  - Hooijberg, E.
A1  - Haanen, J. B. A. G.
A1  - Steenbergen, R. D. M.
T1  - Preclinical safety evaluation of DNA vaccines encoding modified HPV16 E6 and E7
JF  - Vaccine
N2  - Persistent infection with high-risk human papillomaviruses (hrHPV) can result in the formation of anogenital cancers. As hrHPV proteins E6 and E7 are required for cancer initiation and maintenance, they are ideal targets for immunotherapeutic interventions. Previously, we have described the development of DNA vaccines for the induction of HPV16 E6 and E7 specific T cell immunity. These vaccines consist of ‘gene-shuffled’ (SH) versions of HPV16 E6 and E7 that were fused to Tetanus Toxin Fragment C domain 1 (TTFC) and were named TTFC-E6SH and TTFC-E7SH. Gene-shuffling was performed to avoid the risk of inducing malignant transformation at the vaccination site. Here, we describe the preclinical safety evaluation of these candidate vaccines by analysis of their transforming capacity in vitro using established murine fibroblasts (NIH 3T3 cells) and primary human foreskin keratinocytes (HFKs). We demonstrate that neither ectopic expression of TTFC-E6SH and TTFC-E7SH alone or in combination enabled NIH 3T3 cells to form colonies in soft agar. In contrast, expression of HPV16 E6WT and E7WT alone or in combination resulted in effective transformation. Similarly, retroviral transduction of HFKs from three independent donors with both TTFC-E6SH and TTFC-E7SH alone or in combination did not show any signs of immortalization. In contrast, the combined expression of E6WT and E7WT induced immortalization in HFKs from all donors. Based on these results we consider it justified to proceed to clinical evaluation of DNA vaccines encoding TTFC-E6SH and TTFC-E7SH in patients with HPV16 associated (pre)malignancies.
Y1  - 2012
U6  - https://doi.org/10.1016/j.vaccine.2012.04.013
SN  - 0264-410X
VL  - 30
IS  - 28
SP  - 4259
EP  - 4266
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - CHAP
A1  - Biener, Ernst
T1  - Abfallwirtschaft
T2  - Wendehorst Beispiele aus der Baupraxis. - 4. Aufl.
Y1  - 2012
SN  - 978-3-8348-0999-5 ; 978-3-8348-8229-5
U6  - https://doi.org/10.1007/978-3-8348-8229-5_17
SP  - 567
EP  - 603
PB  - Springer Vieweg
CY  - Wiesbaden
ER  - 
TY  - CHAP
A1  - Strohmeier, Andreas
T1  - Siedlungswasserwirtschaft
T2  - Wendehorst Beispiele aus der Baupraxis. - 4. Aufl.
Y1  - 2012
SN  - 978-3-8348-0999-5 ; 978-3-8348-8229-5
U6  - https://doi.org/10.1007/978-3-8348-8229-5_16
SP  - 527
EP  - 566
PB  - Springer Vieweg
CY  - Wiesbaden
ER  - 
TY  - CHAP
A1  - Feiser, Johannes
T1  - Geotechnik
T2  - Wendehorst Beispiele aus der Baupraxis. - 4. Aufl.
Y1  - 2012
SN  - 978-3-8348-0999-5 ; 978-3-8348-8229-5
U6  - https://doi.org/10.1007/978-3-8348-8229-5_14
SP  - 453
EP  - 495
PB  - Springer Vieweg
CY  - Wiesbaden
ER  - 
TY  - CHAP
A1  - Stroetmann, Richard
A1  - Lohse, Wolfram
T1  - Stahlbau
T2  - Wendehorst Beispiele aus der Baupraxis. - 4. Aufl.
Y1  - 2012
SN  - 978-3-8348-0999-5 ; 978-3-8348-8229-5
U6  - https://doi.org/10.1007/978-3-8348-8229-5_10
SP  - 263
EP  - 361
PB  - Springer Vieweg
CY  - Wiesbaden
ER  - 
TY  - CHAP
A1  - Vismann, Ulrich
T1  - Stahlbetonbau
T2  - Wendehorst Beispiele aus der Baupraxis. - 4. Aufl.
Y1  - 2012
SN  - 978-3-8348-0999-5 ; 978-3-8348-8229-5
U6  - https://doi.org/10.1007/978-3-8348-8229-5_9
SP  - 223
EP  - 262
PB  - Springer Vieweg
CY  - Wiesbaden
ER  - 
TY  - BOOK
A1  - Vismann, Ulrich
T1  - Wendehorst Beispiele aus der Baupraxis / Ulrich Vismann (Hrsg.). - 4., durchges. und aktualis. Aufl.
Y1  - 2012
SN  - 978-3-8348-0999-5 ; 978-3-8348-8229-5
U6  - https://doi.org/10.1007/978-3-8348-8229-5
PB  - Springer Vieweg
CY  - Wiesbaden
ER  - 
TY  - CHAP
A1  - Borchert, Jörg
A1  - Michels, A.
ED  - Zenke, ines
T1  - Beschaffungsstrategien an der Schnittstelle Energiehandel / Vertrieb
T2  - Energiehandel in Europa
Y1  - 2012
SN  - 978-3-406-63237-2
SP  - 508
EP  - 522
PB  - Beck
CY  - München
ET  - 3. Aufl.
ER  - 
TY  - CHAP
A1  - Borchert, Jörg
ED  - Zenke, Ines
T1  - Aufbau von Handelseinheiten
T2  - Energiehandel in Europa
Y1  - 2012
SN  - 978-3-406-63237-2
SP  - 467
EP  - 479
PB  - Beck
CY  - München
ET  - 3. Aufl.
ER  -