TY - CHAP A1 - Ritz, Thomas A1 - Strauch, Jakob ED - Back, Andrea T1 - Strukturierte Ermittlung beeinflussender Faktoren für mobile Softwarelösungen T2 - MMS 2012 : mobile und ubiquitäre Informationssysteme ; 7. Konferenz zur "Mobile und Ubiquitäre Informationssysteme" ; 01 - 02. März 2012 in Braunschweig, Germany. - (GI-Edition lecture notes in informatics ; 202) Y1 - 2012 SN - 978-3-88579-296-3 (CD-ROM-Ausg.) PB - Ges. für Informatik CY - Bonn ER - TY - CHAP A1 - Ritz, Thomas A1 - Terhaar, Kristin A1 - Wallenborn, Ramona ED - Reiterer, Harald T1 - HMI für eCarSharing : ein Baustein für nachhaltige Mobilität T2 - Mensch & Computer 2012 - Workshopband : 12. Fachübergreifende Konferenz für interaktive und kooperative Medien ; interaktiv informiert - allgegenwärtig und allumfassend!? Y1 - 2012 SN - 978-3-486-71990-1 (Print) SN - 978-3-486-71991-8 (E-Book) U6 - https://doi.org/10.1524/9783486719918.341 N1 - Workshop Automotive HMI SP - 381 EP - 388 PB - Oldenbourg CY - München ER - TY - CHAP A1 - Rosemann, Michael A1 - Eggert, Mathias A1 - Voigt, Matthias A1 - Beverungen, Daniel T1 - Leveraging Social Network Data for Analytical CRM Strategies - The Introduction of Social BI. T2 - ECIS 2012 Proceedings Y1 - 2012 N1 - European Conference on Information Systems (ECIS), 2012 ER - TY - JOUR A1 - Rost, Kathrin Dorothea A1 - Lind, Thorsten Patric T1 - Die Aufrechnung des Insolvenzverwalters gegen eine Insolvenzforderung nach ihrer Feststellung JF - Zeitschrift für das gesamte Insolvenzrecht : ZInsO Y1 - 2012 SN - 1615-8032 SP - 2179 EP - 2187 PB - Heymanns CY - Köln ER - TY - CHAP A1 - Sauerborn, Markus A1 - Arshadi, S. A1 - Rohrmoser, R. T1 - Influence of clouds and aerosols to the haze of the sunshape T2 - 30th ISES Biennial Solar World Congress 2011 : Kassel, Germany, 28 August - 2 September 2011. Vol. 5 Y1 - 2012 SP - 3887 EP - 3894 PB - Curran CY - Red Hook, NY ER - TY - CHAP A1 - Sauerborn, Markus A1 - Hoffschmidt, Bernhard A1 - Telle, R. A1 - Wagner, M. T1 - Heatable optical analyse system for high temperature absorbers T2 - 30th ISES Biennial Solar World Congress 2011 : : Kassel, Germany, 28 August - 2 September 2011. Vol. 5 Y1 - 2012 SN - 978-1-61839-364-7 SP - 3852 EP - 3860 PB - Curran CY - Red Hook, NY ER - TY - CHAP A1 - Sauerborn, Markus A1 - Klimek, J. A1 - Hoffschmidt, Bernhard A1 - Essen, H. A1 - Sieger, S. A1 - Biegel, G. A1 - Göttsche, Joachim A1 - Hilger, Patrick T1 - Eurosun 2012 : radar technology for heliostat posititon control T2 - Eurosun 2012 : Solar energy for a brighter future : conference proceedings : Rijeka, 18.-22.09.2012 Y1 - 2012 SP - ID 80 CY - Rijeka ER - TY - JOUR A1 - Scheer, Nico A1 - Balimane, Praveen A1 - Hayward, Michael D. A1 - Buechel, Sandra A1 - Kauselmann, Gunther A1 - Wolf, C. Roland T1 - Generation and Characterization of a Novel Multidrug Resistance Protein 2 Humanized Mouse Line JF - Drug Metabolism and Disposition N2 - The multidrug resistance protein (MRP) 2 is predominantly expressed in liver, intestine, and kidney, where it plays an important role in the excretion of a range of drugs and their metabolites or endogenous compounds into bile, feces, and urine. Mrp knockout [Mrp2(−/−)] mice have been used recently to study the role of MRP2 in drug disposition. Here, we describe the first generation and initial characterization of a mouse line humanized for MRP2 (huMRP2), which is nulled for the mouse Mrp2 gene and expresses the human transporter in the organs and cell types where MRP2 is normally expressed. Analysis of the mRNA expression for selected cytochrome P450 and transporter genes revealed no major changes in huMRP2 mice compared with wild-type controls. We show that human MRP2 is able to compensate functionally for the loss of the mouse transporter as demonstrated by comparable bilirubin levels in the humanized mice and wild-type controls, in contrast to the hyperbilirubinemia phenotype that is observed in MRP2(−/−) mice. The huMRP2 mouse provides a model to study the role of the human transporter in drug disposition and in assessing the in vivo consequences of inhibiting this transporter by compounds interacting with human MRP2. Y1 - 2012 U6 - https://doi.org/10.1124/dmd.112.047605 SN - 1521-0111 VL - 40 IS - 11 SP - 2212 EP - 2218 PB - ASPET CY - Bethesda, Md. ER - TY - JOUR A1 - Scheer, Nico A1 - Kapelyukh, Yury A1 - McEwan, Jillian A1 - Beuger, Vincent A1 - Stanley, Lesley A. A1 - Rode, Anja A1 - Wolf, C. Roland T1 - Modeling Human Cytochrome P450 2D6 Metabolism and Drug-drug Interaction by a Novel Panel of Knockout and Humanized Mouse Lines JF - Molecular Pharmacology N2 - The highly polymorphic human cytochrome P450 2D6 enzyme is involved in the metabolism of up to 25% of all marketed drugs and accounts for significant individual differences in response to CYP2D6 substrates. Because of the differences in the multiplicity and substrate specificity of CYP2D family members among species, it is difficult to predict pathways of human CYP2D6-dependent drug metabolism on the basis of animal studies. To create animal models that reflect the human situation more closely and that allow an in vivo assessment of the consequences of differential CYP2D6 drug metabolism, we have developed a novel straightforward approach to delete the entire murine Cyp2d gene cluster and replace it with allelic variants of human CYP2D6. By using this approach, we have generated mouse lines expressing the two frequent human protein isoforms CYP2D6.1 and CYP2D6.2 and an as yet undescribed variant of this enzyme, as well as a Cyp2d cluster knockout mouse. We demonstrate that the various transgenic mouse lines cover a wide spectrum of different human CYP2D6 metabolizer phenotypes. The novel humanization strategy described here provides a robust approach for the expression of different CYP2D6 allelic variants in transgenic mice and thus can help to evaluate potential CYP2D6-dependent interindividual differences in drug response in the context of personalized medicine. Y1 - 2012 U6 - https://doi.org/10.1124/mol.111.075192 SN - 1521-0111 VL - 81 IS - 1 SP - 63 EP - 72 PB - ASPET CY - Bethesda, Md. ER - TY - JOUR A1 - Scheer, Nico A1 - Kapelyukh, Yury A1 - Rode, Anja A1 - Buechel, Sandra A1 - Wolf, C. Roland T1 - Generation and characterization of novel cytochrome P450 Cyp2c gene cluster knockout and CYP2C9 humanized mouse lines JF - Molecular Pharmacology N2 - Compared with rodents and many other animal species, the human cytochrome P450 (P450) Cyp2c gene cluster varies significantly in the multiplicity of functional genes and in the substrate specificity of its enzymes. As a consequence, the use of wild-type animal models to predict the role of human CYP2C enzymes in drug metabolism and drug-drug interactions is limited. Within the human CYP2C cluster CYP2C9 is of particular importance, because it is one of the most abundant P450 enzymes in human liver, and it is involved in the metabolism of a wide variety of important drugs and environmental chemicals. To investigate the in vivo functions of cytochrome P450 Cyp2c genes and to establish a model for studying the functions of CYP2C9 in vivo, we have generated a mouse model with a deletion of the murine Cyp2c gene cluster and a corresponding humanized model expressing CYP2C9 specifically in the liver. Despite the high number of functional genes in the mouse Cyp2c cluster and the reported roles of some of these proteins in different biological processes, mice deleted for Cyp2c genes were viable and fertile but showed certain phenotypic alterations in the liver. The expression of CYP2C9 in the liver also resulted in viable animals active in the metabolism and disposition of a number of CYP2C9 substrates. These mouse lines provide a powerful tool for studying the role of Cyp2c genes and of CYP2C9 in particular in drug disposition and as a factor in drug-drug interaction. Y1 - 2012 U6 - https://doi.org/10.1124/mol.112.080036 SN - 1521-0111 VL - 82 IS - 6 SP - 1022 EP - 1029 PB - ASPET CY - Bethesda, Md. ER -