TY - BOOK A1 - Tünnemann, Thomas T1 - Neo Forma : Experimente. Workshop Formine 2017 N2 - Herausgeber: Prof. Dipl.-Ing. Thomas Tünnemann Katalog Layout; Thilo Haas, Matthias Funken Lektorat: Gerd Götschen Titelbild: Kevin Osterkamp Fotos, Yvonne Albers | Fotografin und die jeweiligen Verfasserinnen Impressum | Inhalt 01 Team | Die Besetzung 03 Thomas Tünnemann | Neo Forma 05 Projekte Carolin Grün | Fare Luce 07 Anke Mannshausen | Tetto Galleggiante 11 Kevin Osterkamp | Azione e Reazione 15 Collin Hackenbroich | Fuori Dentro 21 Lynn Thomas | La Tramoggia 25 Miriam Azzab | l‘Incontro 29 Jonas Wübbe | Le Scale 35 Matthias Funken | Il Laboratorio 39 Moran Dorner | La Via 43 Thilo Haas | Le Scale Vicino 47 Frank Drehsen | Dietro l‘Angelo 51 Felix Fischer | La Capella 55 Thomas Tünnemann | Basilica di Argento 59 Esra Ulutas | Arco Rotondo di Speranza 63 Jana Tillmanns | Punto di Rottura 67 Henri Boh | Il Portale 71 Paul Brüggentisch | Silenzio in Movimento 75 Hermann Stuzmann | Scale Antincendio 79 Atmosphärisches Auf die Küche | Kulinarisches 81 Carpe Diem | Fotografisches 87 Y1 - 2017 SN - 978-3-9811003-9-6 PB - FH Aachen, Fachbereich Architektur CY - Aachen ER - TY - JOUR A1 - Blanke, Tobias A1 - Hagenkamp, Markus A1 - Döring, Bernd A1 - Göttsche, Joachim A1 - Reger, Vitali A1 - Kuhnhenne, Markus T1 - Net-exergetic, hydraulic and thermal optimization of coaxial heat exchangers using fixed flow conditions instead of fixed flow rates JF - Geothermal Energy N2 - Previous studies optimized the dimensions of coaxial heat exchangers using constant mass fow rates as a boundary condition. They show a thermal optimal circular ring width of nearly zero. Hydraulically optimal is an inner to outer pipe radius ratio of 0.65 for turbulent and 0.68 for laminar fow types. In contrast, in this study, fow conditions in the circular ring are kept constant (a set of fxed Reynolds numbers) during optimization. This approach ensures fxed fow conditions and prevents inappropriately high or low mass fow rates. The optimization is carried out for three objectives: Maximum energy gain, minimum hydraulic efort and eventually optimum net-exergy balance. The optimization changes the inner pipe radius and mass fow rate but not the Reynolds number of the circular ring. The thermal calculations base on Hellström’s borehole resistance and the hydraulic optimization on individually calculated linear loss of head coefcients. Increasing the inner pipe radius results in decreased hydraulic losses in the inner pipe but increased losses in the circular ring. The net-exergy diference is a key performance indicator and combines thermal and hydraulic calculations. It is the difference between thermal exergy fux and hydraulic efort. The Reynolds number in the circular ring is instead of the mass fow rate constant during all optimizations. The result from a thermal perspective is an optimal width of the circular ring of nearly zero. The hydraulically optimal inner pipe radius is 54% of the outer pipe radius for laminar fow and 60% for turbulent fow scenarios. Net-exergetic optimization shows a predominant infuence of hydraulic losses, especially for small temperature gains. The exact result depends on the earth’s thermal properties and the fow type. Conclusively, coaxial geothermal probes’ design should focus on the hydraulic optimum and take the thermal optimum as a secondary criterion due to the dominating hydraulics. Y1 - 2021 U6 - http://dx.doi.org/10.1186/s40517-021-00201-3 SN - 2195-9706 N1 - Corresponding author: Tobias Blanke VL - 9 IS - Article number: 19 PB - Springer CY - Berlin ER - TY - JOUR A1 - Picard, Antoni A1 - Brill, Manfred A1 - Cassel, Detlev A1 - Jentsch, Andreas A1 - Rollwa, Markus A1 - Kämper, Klaus-Peter A1 - Merten, Sabine T1 - Neue Medien für die praktische MST-Ausbildung N2 - Im Studiengang Mikrosystemtechnik des Fachhochschulstandortes Zweibrücken werden zwei neue moderne Anlagen für die Herstellung von mikrotechnischen Komponenten in Betrieb genommen: Ein Oxidationsofen für Herstellung dünner Oxidschichten auf Silizium-Einkristallen und eine Belichtungsapparatur für die Fotolithografie - das Besondere an diesen Anlagen: Sie existieren nur virtuell, d.h. als Animationen in einer Computerwelt. KW - Mikrosystemtechnik KW - Photolithographie KW - Mikrotechnik KW - mikrotechnische Komponenten KW - Oxidationsofen KW - Oxidschicht KW - multimediale Lernsoftware KW - micro technology KW - micro-technical components KW - oxidation furnace KW - oxide coating Y1 - 2002 ER - TY - CHAP A1 - Kern, Alexander A1 - Meppelink, Jan T1 - Neue Möglichkeiten elektrischer Anschlüsse an die Bewehrung und Untersuchung der Wirkung von Blitzströmen in bewehrtem Beton N2 - Im Rahmen eines modernen Blitzschutzsystems für Stahlbeton-Bauten bietet es sich an, die Betonbewehrung zu benutzen: - Sie kann die Funktionen der Ableitungseinrichtungen und des Blitzschutz- Potentialausgleichs bei einem klassischen Gebäude-Blitzschutz übernehmen [1]; - Sie kann, ggf. bei entsprechender Ergänzung, als ein geschlossener Käfig ausgebildet werden und damit eine deutliche Reduzierung der Belastung elektrischer / elektronischer Systeme durch blitzinduzierte elektromagnetische Felder erbringen (LEMP-Schutz [2]). Die Nutzung der Bewehrung ist dabei grundsätzlich gleichermaßen bei Neubauten wie auch bei Ertüchtigungen möglich und sinnvoll. So stellt die Nutzung der Bewehrung beispielsweise im Bereich von Großkraftwerken eine wesentliche Ertüchtigungsmaßnahme für den Blitzschutz elektrischer und elektronischer Einrichtungen dar: - Einerseits wird der Blitzschutz-Potentialausgleich durch den Anschluss metallener Einrichtungen wie Elektronik-Schränke, Kabeltrag-Konstruktionen, Rohrleitungen, etc. an die Bewehrung deutlich verbessert. - Andererseits kann bei größeren Gebäuden die elektromagnetische Schirmwirkung durch die elektrische Überbrückung von vorhandenen Dehnfugen bei Stahlbetonbauten optimiert werden. Diese Dehnfugen sind teilweise nur unzureichend überbrückt, so dass bei Blitzeinschlag in das betreffende oder ein benachbartes Gebäude an Kabelstrecken, die über die Dehnfuge hinwegführen, rel. hohe Spannungen induziert werden können [2, 3]. Die sich um das gesamte Gebäude herumziehende oder zwischen zwei Gebäuden befindliche Dehnfuge muss deshalb im Abstand von maximal einigen Metern überbrückt werden. Im Falle von Blitzschutz-Ertüchtigungen in vorhandenen Gebäuden wird bisher an jeder geplanten Anschlussstelle die Bewehrung großflächig (∅ wenige 10 cm) freigelegt, dort ein elektrischer Anschluss zu dem Bewehrungsstab hergestellt, z.B. mittels eines Erdungsfestpunkts, und dann die Betonoberfläche wieder geschlossen. Je nach prognostizierter Strombelastung wird teilweise versucht, den über den Anschluss fließenden Strom bereits auf mehrere Bewehrungsstäbe zu verteilen. Dazu sind entweder die kreuzenden Stäbe zu verschweißen oder es sind direkt Anschlüsse an zwei Bewehrungsstäbe herzustellen. All dieses bedeutet einen hohen Aufwand bei der Freilegung der Bewehrung und auch wieder bei der Schließung der entstandenen Betonlöcher. Es soll deshalb hier untersucht werden, ob es beispielsweise zum Zwecke des Blitzschutz-Potentialausgleichs und auch zur Überbrückung von Dehnfugen ausreichend ist, den Anschluss an die Bewehrung nach einfachen Verfahren nur jeweils an einen Bewehrungsstab herzustellen. Damit würde der finanzielle und administrative Aufwand an Betonarbeiten deutlich reduziert. Die hier dargestellten Verfahren sind dabei insbesondere für den Einsatz bei Blitzschutz-Ertüchtigungen in bestehenden Gebäuden vorgesehen. Abschließend sollen deshalb die Möglichkeiten zur Prüfung korrekter Anschlüsse, die Grenzen der Verfahren sowie auch die Grenzen der Anwendbarkeit bei Neuanlagen diskutiert werden. KW - Blitzschutz KW - Stahlbetonkonstruktion KW - Lightning Protection KW - reinforced concrete Y1 - 2001 ER - TY - JOUR A1 - Falkenberg, Fabian A1 - Voß, Leonie A1 - Bott, Michael A1 - Bongaerts, Johannes A1 - Siegert, Petra T1 - New robust subtilisins from halotolerant and halophilic Bacillaceae JF - Applied Microbiology and Biotechnology N2 - The aim of the present study was the characterisation of three true subtilisins and one phylogenetically intermediate subtilisin from halotolerant and halophilic microorganisms. Considering the currently growing enzyme market for efficient and novel biocatalysts, data mining is a promising source for novel, as yet uncharacterised enzymes, especially from halophilic or halotolerant Bacillaceae, which offer great potential to meet industrial needs. Both halophilic bacteria Pontibacillus marinus DSM 16465ᵀ and Alkalibacillus haloalkaliphilus DSM 5271ᵀ and both halotolerant bacteria Metabacillus indicus DSM 16189 and Litchfieldia alkalitelluris DSM 16976ᵀ served as a source for the four new subtilisins SPPM, SPAH, SPMI and SPLA. The protease genes were cloned and expressed in Bacillus subtilis DB104. Purification to apparent homogeneity was achieved by ethanol precipitation, desalting and ion-exchange chromatography. Enzyme activity could be observed between pH 5.0–12.0 with an optimum for SPPM, SPMI and SPLA around pH 9.0 and for SPAH at pH 10.0. The optimal temperature for SPMI and SPLA was 70 °C and for SPPM and SPAH 55 °C and 50 °C, respectively. All proteases showed high stability towards 5% (w/v) SDS and were active even at NaCl concentrations of 5 M. The four proteases demonstrate potential for future biotechnological applications. KW - Biotechnological application KW - Bacillaceae KW - Subtilisin KW - Subtilases KW - Halotolerant protease Y1 - 2023 U6 - http://dx.doi.org/10.1007/s00253-023-12553-w SN - 1432-0614 N1 - Corresponding author: Petra Siegert VL - 107 SP - 3939 EP - 3954 PB - Springer Nature CY - Berlin ER - TY - CHAP A1 - Gellert, Christoph A1 - Norda, Hannah A1 - Butenweg, Christoph T1 - Nonlinear behaviour of masonry under cyclic loading T2 - 7th European Conference on Structural Dynamics, EURODYN 2008 : 7-9 July 2008, Southampton Y1 - 2008 SP - 1 EP - 12 ER - TY - JOUR A1 - Haeger, Gerrit A1 - Probst, Johanna A1 - Jaeger, Karl-Erich A1 - Bongaerts, Johannes A1 - Siegert, Petra T1 - Novel aminoacylases from Streptomyces griseus DSM 40236 and their recombinant production in Streptomyces lividans JF - FEBS Open Bio N2 - Amino acid-based surfactants are valuable compounds for cosmetic formulations. The chemical synthesis of acyl-amino acids is conventionally performed by the Schotten-Baumann reaction using fatty acyl chlorides, but aminoacylases have also been investigated for use in biocatalytic synthesis with free fatty acids. Aminoacylases and their properties are diverse; they belong to different peptidase families and show differences in substrate specificity and biocatalytic potential. Bacterial aminoacylases capable of synthesis have been isolated from Burkholderia, Mycolicibacterium, and Streptomyces. Although several proteases and peptidases from S. griseus have been described, no aminoacylases from this species have been identified yet. In this study, we investigated two novel enzymes produced by S. griseus DSM 40236ᵀ . We identified and cloned the respective genes and recombinantly expressed an α-aminoacylase (EC 3.5.1.14), designated SgAA, and an ε-lysine acylase (EC 3.5.1.17), designated SgELA, in S. lividans TK23. The purified aminoacylase SgAA was biochemically characterized, focusing on its hydrolytic activity to determine temperature- and pH optima and stabilities. The aminoacylase could hydrolyze various acetyl-amino acids at the Nα -position with a broad specificity regarding the sidechain. Substrates with longer acyl chains, like lauroyl-amino acids, were hydrolyzed to a lesser extent. Purified aminoacylase SgELA specific for the hydrolysis of Nε -acetyl-L-lysine was unstable and lost its enzymatic activity upon storage for a longer period but could initially be characterized. The pH optimum of SgELA was pH 8.0. While synthesis of acyl-amino acids was not observed with SgELA, SgAA catalyzed the synthesis of lauroyl-methionine. KW - Streptomyces lividans KW - recombinant expression KW - Streptomyces griseus KW - ε-lysine acylase KW - α-aminoacylase Y1 - 2023 U6 - http://dx.doi.org/10.1002/2211-5463.13723 SN - 2211-5463 N1 - Corresponding author: Petra Siegert VL - 13 IS - 12 SP - 2224 EP - 2238 PB - Wiley CY - Hoboken, NJ ER - TY - CHAP A1 - Arida, Hassan A. A1 - Kloock, Joachim P. A1 - Schöning, Michael Josef T1 - Novel organic membrane-based thin-film microsensors for the determination of heavy metal cations N2 - A first step towards the fabrication and electrochemical evaluation of thin-film microsensors based on organic PVC membranes for the determination of Hg(II), Cd(II), Pb(II) and Cu(II) ions in solutions has been realised. The membrane-coating mixture used in the preparation of this new type of microsensors is incorporating PVC as supporting matrix, o-nitrophenyloctylether (o-NPOE) as solvent mediator and a recently synthesized Hg[dimethylglyoxime(phene)]2+ and Bis-(4-hydroxyacetophenone)-ethylenediamine as electroactive materials for Hg(II) and Cd(II), respectively. A set of three commercialised ionophores for Cd(II), Pb(II) and Cu(II) has been also used for comparison. Thin-film microsensors based on these membranes showed a Nernstian response of slope (26-30 mV/dec.) for the respective tested cations. The potentiometric response characteristics (linear range, pH range, detection limit and response time) are comparable with those obtained by conventional membranes as well as coated wire electrodes prepared from the same membrane. The realisation of the new organic membrane-based thin-film microsensors overcomes the problem of an insufficient selectivity of solid-state-based thinfilm sensors. KW - Biosensor KW - Heavy metal detection KW - thin-film microsensors KW - organic PVC membranes Y1 - 2006 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:hbz:a96-opus-1545 ER - TY - JOUR A1 - Haeger, Gerrit A1 - Jolmes, Tristan A1 - Oyen, Sven A1 - Jaeger, Karl-Erich A1 - Bongaerts, Johannes A1 - Schörken, Ulrich A1 - Siegert, Petra T1 - Novel recombinant aminoacylase from Paraburkholderia monticola capable of N-acyl-amino acid synthesis JF - Applied Microbiology and Biotechnology N2 - N-Acyl-amino acids can act as mild biobased surfactants, which are used, e.g., in baby shampoos. However, their chemical synthesis needs acyl chlorides and does not meet sustainability criteria. Thus, the identification of biocatalysts to develop greener synthesis routes is desirable. We describe a novel aminoacylase from Paraburkholderia monticola DSM 100849 (PmAcy) which was identified, cloned, and evaluated for its N-acyl-amino acid synthesis potential. Soluble protein was obtained by expression in lactose autoinduction medium and co-expression of molecular chaperones GroEL/S. Strep-tag affinity purification enriched the enzyme 16-fold and yielded 15 mg pure enzyme from 100 mL of culture. Biochemical characterization revealed that PmAcy possesses beneficial traits for industrial application like high temperature and pH-stability. A heat activation of PmAcy was observed upon incubation at temperatures up to 80 °C. Hydrolytic activity of PmAcy was detected with several N-acyl-amino acids as substrates and exhibited the highest conversion rate of 773 U/mg with N-lauroyl-L-alanine at 75 °C. The enzyme preferred long-chain acyl-amino-acids and displayed hardly any activity with acetyl-amino acids. PmAcy was also capable of N-acyl-amino acid synthesis with good conversion rates. The best synthesis results were obtained with the cationic L-amino acids L-arginine and L-lysine as well as with L-leucine and L-phenylalanine. Exemplarily, L-phenylalanine was acylated with fatty acids of chain lengths from C8 to C18 with conversion rates of up to 75%. N-lauroyl-L-phenylalanine was purified by precipitation, and the structure of the reaction product was verified by LC–MS and NMR. KW - Chaperone KW - Biocatalysis KW - Aminoacylase KW - Acylation KW - Acyl-amino acids KW - Biosurfactants Y1 - 2024 U6 - http://dx.doi.org/10.1007/s00253-023-12868-8 SN - 1432-0614 N1 - Corresponding author: Petra Siegert IS - 108 PB - Springer CY - Berlin ER - TY - RPRT A1 - Weßling, Matthias T1 - O Perfil Reiss: Conceito scientifico e conclusões da pesquisa, aplicações exemplaricas e conseqüências práticas T1 - The Reiss-Profile: scientific concept and conclusions of research results, exemplarily applications and practical consequences N2 - Para Reiss, a avaliação de cada um dos 16 motivos vitais é a chave para não só entender o comportamento humano, como também para poder prevê-lo. Tal previsão é também a meta de cada ciência. Quando se quer saber a reação das pessoas, precisa-se primeiro descobrir, o que as pessoas realmente querem – e, então prever, que elas satisfarão esses desejos e necessidades em suas ações. As chances, que o Perfil Reiss oferece, resultam entre outras também, que aqui é possível pela primeira vez uma individualização total. Cerca de 6 bilhões de perfis individuais podem ser apresentados com o Perfil Reiss. Na combinação correta da formação e utilização da tipologia de um lado e da individualidade da conclusão de outro lado, está a força positiva desse instrumento. N2 - Das Reiss-Profil der 16 Lebensmotive ist Ergebnis einer umfassenden, länderübergreifenden empirischen Datenerhebung und beruht auf einer bislang einzigartigen empirischen Forschungsbasis. Es überprüft die Kongruenz zwischen dem Anforderungsprofil eines Stelle und dem Motivationsprofil eines Menschen. Das Reiss-Profil lässt bewusste und unbewusste Motive und Antriebe von Mitarbeitern erkennen, die neue Management- Firmeninitiativen blockieren oder fördern können. Bereits im Vorfeld werden für den einzelnen Mitarbeiter außerordentlich wichtige Werte anhand ihres Motivprofils erkannt und können besser in die Gruppe integriert werden. Im Endergebnis verdeutlicht diese Perspektive für die betriebliche Mitarbeitermotvation eine vollkommene Individualisierung, wenngleich das Ausmaß auch kulturabhängig relativiert werden muß. KW - Motivation KW - Führung KW - Führung KW - Motivation KW - Leadership KW - Motivation KW - Self-Leadership Y1 - 2006 ER -