TY  - JOUR
A1  - Molinnus, Denise
A1  - Hardt, Gabriel
A1  - Siegert, Petra
A1  - Willenberg, Holger S.
A1  - Poghossian, Arshak
A1  - Keusgen, Michael
A1  - Schöning, Michael Josef
T1  - Detection of Adrenaline in Blood Plasma as Biomarker for Adrenal Venous Sampling
JF  - Electroanalysis
N2  - An amperometric bi-enzyme biosensor based on substrate recycling principle for the amplification of the sensor signal has been developed for the detection of adrenaline in blood. Adrenaline can be used as biomarker verifying successful adrenal venous sampling procedure. The adrenaline biosensor has been realized via modification of a galvanic oxygen sensor with a bi-enzyme membrane combining a genetically modified laccase and a pyrroloquinoline quinone-dependent glucose dehydrogenase. The measurement conditions such as pH value and temperature were optimized to enhance the sensor performance. A high sensitivity and a low detection limit of about 0.5–1 nM adrenaline have been achieved in phosphate buffer at pH 7.4, relevant for measurements in blood samples. The sensitivity of the biosensor to other catecholamines such as noradrenaline, dopamine and dobutamine has been studied. Finally, the sensor has been successfully applied for the detection of adrenaline in human blood plasma.
Y1  - 2018
U6  - http://dx.doi.org/10.1002/elan.201800026
SN  - 1521-4109
VL  - 30
IS  - 5
SP  - 937
EP  - 942
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - CHAP
A1  - Molinnus, Denise
A1  - Hardt, Gabriel
A1  - Käver, Larissa
A1  - Willenberg, Holger S.
A1  - Poghossian, Arshak
A1  - Keusgen, Michael
A1  - Schöning, Michael Josef
T1  - Detection of Adrenaline Based on Bioelectrocatalytical System to Support Tumor Diagnostic Technology
T2  - MDPI Proceedings
Y1  - 2017
U6  - http://dx.doi.org/10.3390/proceedings1040506
ER  -