TY - CHAP A1 - Horikawa, Atsushi A1 - Ashikaga, Mitsugu A1 - Yamaguchi, Masato A1 - Ogino, Tomoyuki A1 - Aoki, Shigeki A1 - Wirsum, Manfred A1 - Funke, Harald A1 - Kusterer, Karsten T1 - Combined heat and power supply demonstration of Micro-Mix Hydrogen Combustion Applied to M1A-17 Gas Turbine T2 - Proceedings of ASME Turbo Expo 2022: Turbomachinery Technical Conference and Exposition (GT2022) (Volume 3A) N2 - Kawasaki Heavy Industries, Ltd. (KHI), Aachen University of Applied Sciences, and B&B-AGEMA GmbH have investigated the potential of low NOx micro-mix (MMX) hydrogen combustion and its application to an industrial gas turbine combustor. Engine demonstration tests of a MMX combustor for the M1A-17 gas turbine with a co-generation system were conducted in the hydrogen-fueled power generation plant in Kobe City, Japan. This paper presents the results of the commissioning test and the combined heat and power (CHP) supply demonstration. In the commissioning test, grid interconnection, loading tests and load cut-off tests were successfully conducted. All measurement results satisfied the Japanese environmental regulation values. Dust and soot as well as SOx were not detected. The NOx emissions were below 84 ppmv at 15 % O2. The noise level at the site boundary was below 60 dB. The vibration at the site boundary was below 45 dB. During the combined heat and power supply demonstration, heat and power were supplied to neighboring public facilities with the MMX combustion technology and 100 % hydrogen fuel. The electric power output reached 1800 kW at which the NOx emissions were 72 ppmv at 15 % O2, and 60 %RH. Combustion instabilities were not observed. The gas turbine efficiency was improved by about 1 % compared to a non-premixed type combustor with water injection as NOx reduction method. During a total equivalent operation time of 1040 hours, all combustor parts, the M1A-17 gas turbine as such, and the co-generation system were without any issues. KW - industrial gas turbine KW - combustor development KW - fuels KW - hydrogen KW - emission Y1 - 2022 SN - 978-0-7918-8599-4 U6 - http://dx.doi.org/10.1115/GT2022-81620 N1 - ASME Turbo Expo 2022: Turbomachinery Technical Conference and Exposition June 13–17, 2022 Rotterdam, Netherlands PB - American Society of Mechanical Engineers CY - Fairfield ER - TY - JOUR A1 - Funke, Harald A1 - Beckmann, Nils T1 - Flexible fuel operation of a Dry-Low-NOx Micromix Combustor with Variable Hydrogen Methane Mixture JF - International Journal of Gas Turbine, Propulsion and Power Systems N2 - The role of hydrogen (H2) as a carbon-free energy carrier is discussed since decades for reducing greenhouse gas emissions. As bridge technology towards a hydrogen-based energy supply, fuel mixtures of natural gas or methane (CH4) and hydrogen are possible. The paper presents the first test results of a low-emission Micromix combustor designed for flexible-fuel operation with variable H2/CH4 mixtures. The numerical and experimental approach for considering variable fuel mixtures instead of recently investigated pure hydrogen is described. In the experimental studies, a first generation FuelFlex Micromix combustor geometry is tested at atmospheric pressure at gas turbine operating conditions corresponding to part- and full-load. The H2/CH4 fuel mixture composition is varied between 57 and 100 vol.% hydrogen content. Despite the challenges flexible-fuel operation poses onto the design of a combustion system, the evaluated FuelFlex Micromix prototype shows a significant low NOx performance Y1 - 2022 SN - 1882-5079 VL - 13 IS - 2 SP - 1 EP - 7 ER - TY - CHAP A1 - Tran, Ngoc Trinh A1 - Trinh, Tu Luc A1 - Dao, Ngoc Tien A1 - Giap, Van Tan A1 - Truong, Manh Khuyen A1 - Dinh, Thuy Ha A1 - Staat, Manfred T1 - Limit and shakedown analysis of structures under random strength T2 - Proceedings of (NACOME2022) The 11th National Conference on Mechanics, Vol. 1. Solid Mechanics, Rock Mechanics, Artificial Intelligence, Teaching and Training, Hanoi, December 2-3, 2022 N2 - Direct methods comprising limit and shakedown analysis is a branch of computational mechanics. It plays a significant role in mechanical and civil engineering design. The concept of direct method aims to determinate the ultimate load bearing capacity of structures beyond the elastic range. For practical problems, the direct methods lead to nonlinear convex optimization problems with a large number of variables and onstraints. If strength and loading are random quantities, the problem of shakedown analysis is considered as stochastic programming. This paper presents a method so called chance constrained programming, an effective method of stochastic programming, to solve shakedown analysis problem under random condition of strength. In this our investigation, the loading is deterministic, the strength is distributed as normal or lognormal variables. KW - Reliability of structures KW - Stochastic programming KW - Chance constrained programming KW - Shakedown analysis KW - Limit analysis Y1 - 2022 SN - 978-604-357-084-7 SP - 510 EP - 518 PB - Nha xuat ban Khoa hoc tu nhien va Cong nghe (Verlag Naturwissenschaft und Technik) CY - Hanoi ER - TY - CHAP A1 - Milijaš, Aleksa A1 - Šakić, Bogdan A1 - Marinković, Marko A1 - Butenweg, Christoph A1 - Gams, Matija A1 - Klinkel, Sven ED - Arion, Cristian ED - Scupin, Alexandra ED - Ţigănescu, Alexandru T1 - Effects of prior in-plane damage on out-of-plane response of masonry infills with openings T2 - The Third European Conference on Earthquake Engineering and Seismology September 4 – September 9, 2022, Bucharest N2 - Masonry infill walls are the most traditional enclosure system that is still widely used in RC frame buildings all over the world, particularly in seismic active regions. Although infill walls are usually neglected in seismic design, during an earthquake event they are subjected to in-plane and out-of-plane forces that can act separately or simultaneously. Since observations of damage to buildings after recent earthquakes showed detrimental effects of in-plane and out-of-plane load interaction on infill walls, the number of studies that focus on influence of in-plane damage on out-of-plane response has significantly increased. However, most of the xperimental campaigns have considered only solid infills and there is a lack of combined in-plane and out-of-plane experimental tests on masonry infills with openings, although windows and doors strongly affect seismic performance. In this paper, two types of experimental tests on infills with window openings are presented. The first is a pure out-of-plane test and the second one is a sequential in-plane and out-of-plane test aimed at investigating the effects of existing in-plane damage on outof-plane response. Additionally, findings from two tests with similar load procedure that were carried out on fully infilled RC frames in the scope of the same project are used for comparison. Test results clearly show that window opening increased vulnerability of infills to combined seismic actions and that prevention of damage in infills with openings is of the utmost importance for seismic safety. KW - Seismic loading KW - In-plane load KW - Out-of-plane load KW - Interaction KW - Window opening Y1 - 2022 SN - 978-973-100-533-1 SP - 2747 EP - 2756 ER - TY - CHAP A1 - Riga, Evi A1 - Pitilakis, Kyriazis A1 - Butenweg, Christoph A1 - Apostolaki, Stefania A1 - Karatzetzou, Anna ED - Arion, Cristian ED - Scupin, Alexandra ED - Ţigănescu, Alexandru T1 - Investigating the impact of the new European Seismic Hazard Model ESHM20 on the seismic design and safety control of industrial facilities T2 - The Third European Conference on Earthquake Engineering and Seismology September 4 – September 9, 2022, Bucharest N2 - The seismic performance and safety of major European industrial facilities has a global interest for Europe, its citizens and economy. A potential major disaster at an industrial site could affect several countries, probably far beyond the country where it is located. However, the seismic design and safety assessment of these facilities is practically based on national, often outdated seismic hazard assessment studies, due to many reasons, including the absence of a reliable, commonly developed seismic hazard model for whole Europe. This important gap is no more existing, as the 2020 European Seismic Hazard Model ESHM20 was released in December 2021. In this paper we investigate the expected impact of the adoption of ESHM20 on the seismic demand for industrial facilities, through the comparison of the ESHM20 probabilistic hazard at the sites where industrial facilities are located with the respective national and European regulations. The goal of this preliminary work in the framework of Working Group 13 of the European Association for Earthquake Engineering (EAEE), is to identify potential inadequacies in the design and safety control of existing industrial facilities and to highlight the expected impact of the adoption of the new European Seismic Hazard Model on the design of new industrial facilities and the safety assessment of existing ones. KW - ESHM20, industrial facilities KW - seismic hazard KW - seismic design KW - safety control Y1 - 2022 SN - 978-973-100-533-1 SP - 3261 EP - 3270 ER - TY - CHAP A1 - Pütz, Sebastian A1 - Baier, Ralph A1 - Brauner, Philipp A1 - Brillowski, Florian A1 - Dammers, Hannah A1 - Liehner, Luca A1 - Mertens, Alexander A1 - Rodemann, Niklas A1 - Schneider, Sebastian A1 - Schollemann, Alexander A1 - Steuer-Dankert, Linda A1 - Vervier, Luisa A1 - Gries, Thomas A1 - Leicht-Scholten, Carmen A1 - Nagel, Saskia K. A1 - Piller, Frank T. A1 - Schuh, Günther A1 - Ziefle, Martina A1 - Nitsch, Verena T1 - An interdisciplinary view on humane interfaces for digital shadows in the internet of production T2 - 2022 15th International Conference on Human System Interaction (HSI) N2 - Digital shadows play a central role for the next generation industrial internet, also known as Internet of Production (IoP). However, prior research has not considered systematically how human actors interact with digital shadows, shaping their potential for success. To address this research gap, we assembled an interdisciplinary team of authors from diverse areas of human-centered research to propose and discuss design and research recommendations for the implementation of industrial user interfaces for digital shadows, as they are currently conceptualized for the IoP. Based on the four use cases of decision support systems, knowledge sharing in global production networks, human-robot collaboration, and monitoring employee workload, we derive recommendations for interface design and enhancing workers’ capabilities. This analysis is extended by introducing requirements from the higher-level perspectives of governance and organization. KW - digital twin KW - digital shadow KW - cyber-physical production system KW - human-machine interface Y1 - 2022 SN - 978-1-6654-6823-7 (Print) SN - 978-1-6654-6822-0 (Online) U6 - http://dx.doi.org/10.1109/HSI55341.2022.9869467 SN - 2158-2246 (Print) SN - 2158-2254 (Online) N1 - 15th International Conference on Human System Interaction (HSI), 28-31 July 2022, Melbourne, Australia. PB - IEEE ER - TY - JOUR A1 - Falkenberg, Fabian A1 - Bott, Michael A1 - Bongaerts, Johannes A1 - Siegert, Petra T1 - Phylogenetic survey of the subtilase family and a data-mining-based search for new subtilisins from Bacillaceae JF - Frontiers in Microbiology N2 - The subtilase family (S8), a member of the clan SB of serine proteases are ubiquitous in all kingdoms of life and fulfil different physiological functions. Subtilases are divided in several groups and especially subtilisins are of interest as they are used in various industrial sectors. Therefore, we searched for new subtilisin sequences of the family Bacillaceae using a data mining approach. The obtained 1,400 sequences were phylogenetically classified in the context of the subtilase family. This required an updated comprehensive overview of the different groups within this family. To fill this gap, we conducted a phylogenetic survey of the S8 family with characterised holotypes derived from the MEROPS database. The analysis revealed the presence of eight previously uncharacterised groups and 13 subgroups within the S8 family. The sequences that emerged from the data mining with the set filter parameters were mainly assigned to the subtilisin subgroups of true subtilisins, high-alkaline subtilisins, and phylogenetically intermediate subtilisins and represent an excellent source for new subtilisin candidates. Y1 - 2022 U6 - http://dx.doi.org/10.3389/fmicb.2022.1017978 SN - 1664-302X VL - 2022 IS - 13 PB - Frontiers CY - Lausanne ER - TY - JOUR A1 - Haeger, Gerrit A1 - Bongaerts, Johannes A1 - Siegert, Petra T1 - A convenient ninhydrin assay in 96-well format for amino acid-releasing enzymes using an air-stable reagent JF - Analytical Biochemistry N2 - An improved and convenient ninhydrin assay for aminoacylase activity measurements was developed using the commercial EZ Nin™ reagent. Alternative reagents from literature were also evaluated and compared. The addition of DMSO to the reagent enhanced the solubility of Ruhemann's purple (RP). Furthermore, we found that the use of a basic, aqueous buffer enhances stability of RP. An acidic protocol for the quantification of lysine was developed by addition of glacial acetic acid. The assay allows for parallel processing in a 96-well format with measurements microtiter plates. Y1 - 2022 U6 - http://dx.doi.org/10.1016/j.ab.2022.114819 SN - 1096-0309 IS - 624 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Welden, Melanie A1 - Severins, Robin A1 - Poghossian, Arshak A1 - Wege, Christina A1 - Bongaerts, Johannes A1 - Siegert, Petra A1 - Keusgen, Michael A1 - Schöning, Michael Josef T1 - Detection of acetoin and diacetyl by a tobacco mosaic virus-assisted field-effect biosensor JF - Chemosensors N2 - Acetoin and diacetyl have a major impact on the flavor of alcoholic beverages such as wine or beer. Therefore, their measurement is important during the fermentation process. Until now, gas chromatographic techniques have typically been applied; however, these require expensive laboratory equipment and trained staff, and do not allow for online monitoring. In this work, a capacitive electrolyte–insulator–semiconductor sensor modified with tobacco mosaic virus (TMV) particles as enzyme nanocarriers for the detection of acetoin and diacetyl is presented. The enzyme acetoin reductase from Alkalihalobacillus clausii DSM 8716ᵀ is immobilized via biotin–streptavidin affinity, binding to the surface of the TMV particles. The TMV-assisted biosensor is electrochemically characterized by means of leakage–current, capacitance–voltage, and constant capacitance measurements. In this paper, the novel biosensor is studied regarding its sensitivity and long-term stability in buffer solution. Moreover, the TMV-assisted capacitive field-effect sensor is applied for the detection of diacetyl for the first time. The measurement of acetoin and diacetyl with the same sensor setup is demonstrated. Finally, the successive detection of acetoin and diacetyl in buffer and in diluted beer is studied by tuning the sensitivity of the biosensor using the pH value of the measurement solution. Y1 - 2022 U6 - http://dx.doi.org/10.3390/chemosensors10060218 SN - 2227-9040 N1 - This article belongs to the Special Issue "Nanostructured Devices for Biochemical Sensing" VL - 10 IS - 6 PB - MDPI CY - Basel ER - TY - CHAP A1 - Welden, Melanie A1 - Severins, Robin A1 - Poghossian, Arshak A1 - Wege, Christina A1 - Siegert, Petra A1 - Keusgen, Michael A1 - Schöning, Michael Josef T1 - Studying the immobilization of acetoin reductase with Tobacco mosaic virus particles on capacitive field-effect sensors T2 - 2022 IEEE International Symposium on Olfaction and Electronic Nose (ISOEN) N2 - A capacitive electrolyte-insulator-semiconductor (EISCAP) biosensor modified with Tobacco mosaic virus (TMV) particles for the detection of acetoin is presented. The enzyme acetoin reductase (AR) was immobilized on the surface of the EISCAP using TMV particles as nanoscaffolds. The study focused on the optimization of the TMV-assisted AR immobilization on the Ta 2 O 5 -gate EISCAP surface. The TMV-assisted acetoin EISCAPs were electrochemically characterized by means of leakage-current, capacitance-voltage, and constant-capacitance measurements. The TMV-modified transducer surface was studied via scanning electron microscopy. KW - Tobacco mosaic virus KW - acetoin KW - capacitive field-effect biosensor KW - enzyme immobilization Y1 - 2022 SN - 978-1-6654-5860-3 (Online) SN - 978-1-6654-5861-0 (Print) U6 - http://dx.doi.org/10.1109/ISOEN54820.2022.9789657 N1 - IEEE International Symposium on Olfaction and Electronic Nose (ISOEN), 29 May 2022 - 01 June 2022, Aveiro, Portugal. PB - IEEE ER -