TY - JOUR A1 - Falkenberg, Fabian A1 - Kohn, Sophie A1 - Bott, Michael A1 - Bongaerts, Johannes A1 - Siegert, Petra T1 - Biochemical characterisation of a novel broad pH spectrum subtilisin from Fictibacillus arsenicus DSM 15822ᵀ JF - FEBS Open Bio N2 - Subtilisins from microbial sources, especially from the Bacillaceae family, are of particular interest for biotechnological applications and serve the currently growing enzyme market as efficient and novel biocatalysts. Biotechnological applications include use in detergents, cosmetics, leather processing, wastewater treatment and pharmaceuticals. To identify a possible candidate for the enzyme market, here we cloned the gene of the subtilisin SPFA from Fictibacillus arsenicus DSM 15822ᵀ (obtained through a data mining-based search) and expressed it in Bacillus subtilis DB104. After production and purification, the protease showed a molecular mass of 27.57 kDa and a pI of 5.8. SPFA displayed hydrolytic activity at a temperature optimum of 80 °C and a very broad pH optimum between 8.5 and 11.5, with high activity up to pH 12.5. SPFA displayed no NaCl dependence but a high NaCl tolerance, with decreasing activity up to concentrations of 5 m NaCl. The stability enhanced with increasing NaCl concentration. Based on its substrate preference for 10 synthetic peptide 4-nitroanilide substrates with three or four amino acids and its phylogenetic classification, SPFA can be assigned to the subgroup of true subtilisins. Moreover, SPFA exhibited high tolerance to 5% (w/v) SDS and 5% H₂O₂ (v/v). The biochemical properties of SPFA, especially its tolerance of remarkably high pH, SDS and H₂O₂, suggest it has potential for biotechnological applications. KW - Bacillaceae KW - Biotechnological application KW - Broad pH spectrum KW - Subtilases KW - Subtilisin Y1 - 2023 U6 - http://dx.doi.org/10.1002/2211-5463.13701 SN - 2211-5463 N1 - Corresponding author: Petra Siegert VL - 13 IS - 11 SP - 2035 EP - 2046 PB - Wiley CY - Hoboken, NJ ER - TY - CHAP A1 - Schulze-Buxloh, Lina A1 - Groß, Rolf Fritz A1 - Ulbrich, Michelle T1 - Digital planning using building information modelling and virtual reality: new approach for students’ remote practical training under lockdown conditions in the course of smart building engineering T2 - Proceedings of International Conference on Education in Mathematics, Science and Technology 2021 N2 - The worldwide Corona pandemic has severely restricted student projects in the higher semesters of engineering courses. In order not to delay the graduation, a new concept had to be developed for projects under lockdown conditions. Therefore, unused rooms at the university should be digitally recorded in order to develop a new usage concept as laboratory rooms. An inventory of the actual state of the rooms was done first by taking photos and listing up all flaws and peculiarities. After that, a digital site measuring was done with a 360° laser scanner and these recorded scans were linked to a coherent point cloud and transferred to a software for planning technical building services and supporting Building Information Modelling (BIM). In order to better illustrate the difference between the actual and target state, two virtual reality models were created for realistic demonstration. During the project, the students had to go through the entire digital planning phases. Technical specifications had to be complied with, as well as documentation, time planning and cost estimate. This project turned out to be an excellent alternative to on-site practical training under lockdown conditions and increased the students’ motivation to deal with complex technical questions. KW - smart building engineering KW - building information modelling KW - virtual reality KW - lockdown conditions KW - emote practical training Y1 - 2021 SN - 978-1-952092-17-6 N1 - April 1-4, 2021 in Antalya, Turkey. SP - 118 EP - 123 PB - ISTES Organization CY - San Antonio, TX ER - TY - CHAP A1 - Schulze-Buxloh, Lina A1 - Groß, Rolf Fritz T1 - Interdisciplinary Course Smart Building Engineering: A new approach of teaching freshmen in remote teamwork project under pandemic restrictions T2 - New Perspectives in Science Education -International Conference (virtual event, 18-19 March 2021) N2 - In the context of the Corona pandemic and its impact on teaching like digital lectures and exercises a new concept especially for freshmen in demanding courses of Smart Building Engineering became necessary. As there were hardly any face-to-face events at the university, the new teaching concept should enable a good start into engineering studies under pandemic conditions anyway and should also replace the written exam at the end. The students should become active themselves in small teams instead of listening passively to a lecture broadcast online with almost no personal contact. For this purpose, a role play was developed in which the freshmen had to work out a complete solution to the realistic problem of designing, construction planning and implementing a small guesthouse. Each student of the team had to take a certain role like architect, site manager, BIM-manager, electrician and the technitian for HVAC installations. Technical specifications must be complied with, as well as documentation, time planning and cost estimate. The final project folder had to contain technical documents like circuit diagrams for electrical components, circuit diagrams for water and heating, design calculations and components lists. On the other hand construction schedule, construction implementation plan, documentation of the construction progress and minutes of meetings between the various trades had to be submitted as well. In addition to the project folder, a model of the construction project must also be created either as a handmade model or as a digital 3D-model using Computer-aided design (CAD) software. The first steps in the field of Building information modelling (BIM) had also been taken by creating a digital model of the building showing the current planning status in real time as a digital twin. This project turned out to be an excellent training of important student competencies like teamwork, communication skills, and self -organisation and also increased motivation to work on complex technical questions. The aim of giving the student a first impression on the challenges and solutions in building projects with many different technical trades and their points of view was very well achieved and should be continued in the future. KW - Freshmen KW - roleplay KW - Smart Building Engineering KW - BIM KW - remote teamwork Y1 - 2021 N1 - New Perspectives in Science Education - 10th Edition 18-19 March 2021 Fully Virtual Conference PB - Filodiritto CY - Bologna ER - TY - THES A1 - Gaigall, Daniel T1 - On selected problems in multivariate analysis N2 - Selected problems in the field of multivariate statistical analysis are treated. Thereby, one focus is on the paired sample case. Among other things, statistical testing problems of marginal homogeneity are under consideration. In detail, properties of Hotelling‘s T² test in a special parametric situation are obtained. Moreover, the nonparametric problem of marginal homogeneity is discussed on the basis of possibly incomplete data. In the bivariate data case, properties of the Hoeffding-Blum-Kiefer-Rosenblatt independence test statistic on the basis of partly not identically distributed data are investigated. Similar testing problems are treated within the scope of the application of a result for the empirical process of the concomitants for partly categorial data. Furthermore, testing changes in the modeled solvency capital requirement of an insurance company by means of a paired sample from an internal risk model is discussed. Beyond the paired sample case, a new asymptotic relative efficiency concept based on the expected volumes of multidimensional confidence regions is introduced. Besides, a new approach for the treatment of the multi-sample goodness-of-fit problem is presented. Finally, a consistent test for the treatment of the goodness-of-fit problem is developed for the background of huge or infinite dimensional data. KW - Paired sample KW - Marginal homogeneity KW - Incomplete data KW - Asymptotic relative efficiency KW - Volumes of confidence regions Y1 - 2023 U6 - http://dx.doi.org/10.15488/14304 N1 - Gottfried Wilhelm Leibniz Universität Hannover ER - TY - JOUR A1 - Cheenakula, Dheeraja A1 - Paulsen, Svea A1 - Ott, Fabian A1 - Grömping, Markus T1 - Operational window of a deammonifying sludge for mainstream application in a municipal wastewater treatment plant JF - Water and Environment Journal N2 - The present work aimed to study the mainstream feasibility of the deammonifying sludge of side stream of municipal wastewater treatment plant (MWWTP) in Kaster, Germany. For this purpose, the deammonifying sludge available at the side stream was investigated for nitrogen (N) removal with respect to the operational factors temperature (15–30°C), pH value (6.0–8.0) and chemical oxygen demand (COD)/N ratio (≤1.5–6.0). The highest and lowest N-removal rates of 0.13 and 0.045 kg/(m³ d) are achieved at 30 and 15°C, respectively. Different conditions of pH and COD/N ratios in the SBRs of Partial nitritation/anammox (PN/A) significantly influenced both the metabolic processes and associated N-removal rates. The scientific insights gained from the current work signifies the possibility of mainstream PN/A at WWTPs. The current study forms a solid basis of operational window for the upcoming semi-technical trails to be conducted prior to the full-scale mainstream PN/A at WWTP Kaster and WWTPs globally. KW - Anammox KW - Mainstream KW - Nitrogen removal KW - Partial nitritation KW - Wastewater Y1 - 2023 U6 - http://dx.doi.org/10.1111/wej.12898 SN - 1747-6593 N1 - Corresponding author: Dheeraja Cheenakula IS - Early View SP - 1 EP - 12 PB - Wiley CY - Chichester ER - TY - CHAP A1 - Küppers, Malte A1 - Schuba, Marko A1 - Neugebauer, Georg A1 - Höner, Tim A1 - Hack, Sacha T1 - Security analysis of the KNX smart building protocol T2 - ARES '23: Proceedings of the 18th International Conference on Availability, Reliability and Security N2 - KNX is a protocol for smart building automation, e.g., for automated heating, air conditioning, or lighting. This paper analyses and evaluates state-of-the-art KNX devices from manufacturers Merten, Gira and Siemens with respect to security. On the one hand, it is investigated if publicly known vulnerabilities like insecure storage of passwords in software, unencrypted communication, or denialof-service attacks, can be reproduced in new devices. On the other hand, the security is analyzed in general, leading to the discovery of a previously unknown and high risk vulnerability related to so-called BCU (authentication) keys. Y1 - 2023 U6 - http://dx.doi.org/10.1145/3600160.3605167 N1 - Article No.: 87 ARES 2023, August 29–September 01, 2023, Benevento, Italy SP - 1 EP - 7 PB - ACM ER - TY - GEN A1 - Schulze-Buxloh, Lina A1 - Groß, Rolf Fritz A1 - Cheng, Kevin Toni T1 - Development and manufacturing of an interactive three-dimensional phase diagram of carbon dioxide for teaching sessions in thermodynamics T2 - Proceedings of the International Conference The Future of Education 2020 Y1 - 2020 ER - TY - CHAP A1 - Galdi, Chiara A1 - Hartung, Frank A1 - Dugelay, Jean-Luc T1 - Videos versus still images: Asymmetric sensor pattern noise comparison on mobile phones T2 - Electronic Imaging N2 - Nowadays, the most employed devices for recoding videos or capturing images are undoubtedly the smartphones. Our work investigates the application of source camera identification on mobile phones. We present a dataset entirely collected by mobile phones. The dataset contains both still images and videos collected by 67 different smartphones. Part of the images consists in photos of uniform backgrounds, especially collected for the computation of the RSPN. Identifying the source camera given a video is particularly challenging due to the strong video compression. The experiments reported in this paper, show the large variation in performance when testing an highly accurate technique on still images and videos. KW - Image Forensics KW - Mobile Phones KW - Image Database Y1 - 2017 U6 - http://dx.doi.org/10.2352/ISSN.2470-1173.2017.7.MWSF-331 SN - 2470-1173 N1 - IS&T International Symposium on Electronic Imaging 2017 Media Watermarking, Security, and Forensics 2017 SP - 100 EP - 103 PB - Society for Imaging Science and Technology CY - Springfield, Virginia ER - TY - JOUR A1 - Thoma, Andreas A1 - Thomessen, Karolin A1 - Gardi, Alessandro A1 - Fisher, A. A1 - Braun, Carsten T1 - Prioritising paths: An improved cost function for local path planning for UAV in medical applications JF - The Aeronautical Journal N2 - Even the shortest flight through unknown, cluttered environments requires reliable local path planning algorithms to avoid unforeseen obstacles. The algorithm must evaluate alternative flight paths and identify the best path if an obstacle blocks its way. Commonly, weighted sums are used here. This work shows that weighted Chebyshev distances and factorial achievement scalarising functions are suitable alternatives to weighted sums if combined with the 3DVFH* local path planning algorithm. Both methods considerably reduce the failure probability of simulated flights in various environments. The standard 3DVFH* uses a weighted sum and has a failure probability of 50% in the test environments. A factorial achievement scalarising function, which minimises the worst combination of two out of four objective functions, reaches a failure probability of 26%; A weighted Chebyshev distance, which optimises the worst objective, has a failure probability of 30%. These results show promise for further enhancements and to support broader applicability. KW - Path planning KW - Cost function KW - Multi-objective optimization Y1 - 2023 U6 - http://dx.doi.org/10.1017/aer.2023.68 SN - 0001-9240 (Print) SN - 2059-6464 (Online) IS - First View SP - 1 EP - 18 PB - Cambridge University Press CY - Cambridge ER - TY - CHAP A1 - Dachwald, Bernd A1 - Ulamec, Stephan A1 - Kowalski, Julia A1 - Boxberg, Marc S. A1 - Baader, Fabian A1 - Biele, Jens A1 - Kömle, Norbert ED - Badescu, Viorel ED - Zacny, Kris ED - Bar-Cohen, Yoseph T1 - Ice melting probes T2 - Handbook of Space Resources N2 - The exploration of icy environments in the solar system, such as the poles of Mars and the icy moons (a.k.a. ocean worlds), is a key aspect for understanding their astrobiological potential as well as for extraterrestrial resource inspection. On these worlds, ice melting probes are considered to be well suited for the robotic clean execution of such missions. In this chapter, we describe ice melting probes and their applications, the physics of ice melting and how the melting behavior can be modeled and simulated numerically, the challenges for ice melting, and the required key technologies to deal with those challenges. We also give an overview of existing ice melting probes and report some results and lessons learned from laboratory and field tests. KW - Ice melting probe KW - Ice penetration KW - Icy moons KW - Ocean worlds KW - Mars Y1 - 2023 SN - 978-3-030-97912-6 (Print) SN - 978-3-030-97913-3 (Online) U6 - http://dx.doi.org/10.1007/978-3-030-97913-3_29 SP - 955 EP - 996 PB - Springer CY - Cham ER -