TY  - JOUR
A1  - Jordan, Sabine D.
A1  - Krüger, Markus
A1  - Willmes, Diana M.
A1  - Redemann, Nora
A1  - Wunderlich, F. Thomas
A1  - Brönneke, Hella S.
A1  - Merkwirth, Carsten
A1  - Kashkar, Hamid
A1  - Olkkonen, Vesa M.
A1  - Böttger, Thomas
A1  - Braun, Thomas
A1  - Seibler, Jost
A1  - Brüning, Jens C.
T1  - Obesity-induced overexpression of miRNA-143 inhibits insulin-stimulated AKT activation and impairs glucose metabolism
JF  - Nature Cell Biology
N2  - The contribution of altered post-transcriptional gene silencing to the development of insulin resistance and type 2 diabetes mellitus so far remains elusive. Here, we demonstrate that expression of microRNA (miR)-143 and 145 is upregulated in the liver of genetic and dietary mouse models of obesity. Induced transgenic overexpression of miR-143, but not miR-145, impairs insulin-stimulated AKT activation and glucose homeostasis. Conversely, mice deficient for the miR-143–145 cluster are protected from the development of obesity-associated insulin resistance. Quantitative-mass-spectrometry-based analysis of hepatic protein expression in miR-143-overexpressing mice revealed miR-143-dependent downregulation of oxysterol-binding-protein-related protein (ORP) 8. Reduced ORP8 expression in cultured liver cells impairs the ability of insulin to induce AKT activation, revealing an ORP8-dependent mechanism of AKT regulation. Our experiments provide direct evidence that dysregulated post-transcriptional gene silencing contributes to the development of obesity-induced insulin resistance, and characterize the miR-143–ORP8 pathway as a potential target for the treatment of obesity-associated diabetes.
Y1  - 2011
U6  - http://dx.doi.org/10.1038/ncb2211
SN  - 1465-7392
VL  - 13
IS  - 4
SP  - 434
EP  - 446
PB  - Nature
CY  - New York
ER  - 
TY  - JOUR
A1  - Hasegawa, Maki
A1  - Kapelyukh, Yury
A1  - Tahara, Harunobu
A1  - Seibler, Jost
A1  - Rode, Anja
A1  - Krueger, Sylvia
A1  - Lee, Dongtao N.
A1  - Wolf, C. Roland
A1  - Scheer, Nico
T1  - Quantitative prediction of human pregnane X receptor and cytochrome P450 3A4 mediated drug-drug interaction in a novel multiple humanized mouse line
JF  - Molecular Pharmacology
Y1  - 2011
U6  - http://dx.doi.org/10.1124/mol.111.071845
SN  - 1521-0111
VL  - 80
IS  - 33
SP  - 518
EP  - 528
PB  - ASPET
CY  - Bethesda, Md.
ER  - 
TY  - JOUR
A1  - Raab, Monika
A1  - Kappel, Sven
A1  - Krämer, Andrea
A1  - Sanhaji, Mourad
A1  - Matthess, Yves
A1  - Kurunci-Csacsko, Elisabeth
A1  - Calzada-Wack, Julia
A1  - Rathkolb, Birgit
A1  - Rosman, Jan
A1  - Adler, Thure
A1  - Busch, Dirk H.
A1  - Esposito, Irene
A1  - Fuchs, Helmut
A1  - Gailus-Durner, Valérie
A1  - Klingenspor, Martin
A1  - Wolf, Eckhard
A1  - Sänger, Nicole
A1  - Prinz, Florian
A1  - Hrabe de Angelis, Martin
A1  - Seibler, Jost
A1  - Yuan, Juping
A1  - Bergmann, Martin
A1  - Knecht, Rainald
A1  - Kreft, Bertolt
A1  - Strebhardt, Klaus
T1  - Toxicity modelling of Plk1-targeted therapies in genetically engineered mice and cultured primary mammalian cells
JF  - Nature Communications
Y1  - 2011
U6  - http://dx.doi.org/10.1038/ncomms1395
SN  - 2041-1723
VL  - 2
IS  - 395
SP  - 1
EP  - 11
PB  - Nature
CY  - London
ER  - 
TY  - JOUR
A1  - Inagaki, Akiko
A1  - Sleddens-Linkels, Esther
A1  - Wassenaar, Evelyne
A1  - Ooms, Marja
A1  - Cappellen, Wiggert A. van
A1  - Hoeijmakers, Jan H. J.
A1  - Seibler, Jost
A1  - Vogt, Thomas F.
A1  - Shin, Myung K.
A1  - Grootegoed, J. Anton
A1  - Baarends, Willy M.
T1  - Meiotic functions of RAD18
JF  - Journal of Cell Science
Y1  - 2011
U6  - http://dx.doi.org/10.1242/jcs.081968
SN  - 1477-9137
VL  - 124
IS  - 16
SP  - 2837
EP  - 2850
PB  - Company of Biologists Limited
CY  - Cambridge
ER  - 
TY  - JOUR
A1  - Kornfeld, Jan-Wilhelm
A1  - Baitzel, Catherina
A1  - Könner, A. Christine
A1  - Nicholls, Hayley T.
A1  - Vogt, Merly C.
A1  - Herrmanns, Karolin
A1  - Scheja, Ludger
A1  - Haumaitre, Cécile
A1  - Wolf, Anna M.
A1  - Knippschild, Uwe
A1  - Seibler, Jost
A1  - Cereghini, Silvia
A1  - Heeren, Joerg
A1  - Stoffel, Markus
A1  - Brüning, Jens C.
T1  - Obesity-induced overexpression of miR-802 impairs glucose metabolism through silencing of Hnf1b
JF  - Nature
Y1  - 2013
SN  - 0028-0836
U6  - http://dx.doi.org/10.1038/nature11793
VL  - 494
IS  - 7435
SP  - 111
EP  - 115
PB  - Springer Nature
CY  - Cham
ER  - 
TY  - JOUR
A1  - Gebeshuber, Christoph A.
A1  - Kornauth, Christoph
A1  - Dong, Lihua
A1  - Sierig, Ralph
A1  - Seibler, Jost
A1  - Reiss, Martina
A1  - Tauber, Stefanie
A1  - Bilban, Martin
A1  - Wang, Shijun
A1  - Kain, Renate
A1  - Böhmig, Georg A.
A1  - Moeller, Marcus J.
A1  - Gröne, Hermann-Josef
A1  - Englert, Christoph
A1  - Martinez, Javier
A1  - Kerjaschki, Dontscho
T1  - Focal segmental glomerulosclerosis is induced by microRNA-193a and its downregulation of WT1
JF  - Nature Medicine
Y1  - 2013
U6  - http://dx.doi.org/10.1038/nm.3142
SN  - 1078-8956
VL  - 19
IS  - 4
SP  - 481
EP  - 487
ER  - 
TY  - JOUR
A1  - Michalak, Ewa Malgorzata
A1  - Nacerddine, Karim
A1  - Pietersen, Alexandra
A1  - Beuger, Vincent
A1  - Pawlitzky, Inka
A1  - Cornelissen-Steijger, Paulien
A1  - Wientjens, Ellen
A1  - Tanger, Ellen
A1  - Seibler, Jost
A1  - Lohuizen, Maarten van
A1  - Jonkers, Jos
T1  - Polycomb group gene Ezh2 regulates mammary gland morphogenesis and maintains the luminal progenitor pool
JF  - Stem Cells
Y1  - 2013
U6  - http://dx.doi.org/10.1002/stem.1437
SN  - 1549-4918
VL  - Vol 31
IS  - 9
SP  - 1910
EP  - 1920
PB  - Oxford University Press
CY  - Oxford
ER  - 
TY  - JOUR
A1  - Bouwman, Peter
A1  - Gulden, Hanneke van der
A1  - Heijden, Ingrid van der
A1  - Drost, Rinske
A1  - Klijn, Christiaan N.
A1  - Prasetyanti, Pramudita
A1  - Pieterse, Mark
A1  - Wientjens, Ellen
A1  - Seibler, Jost
A1  - Hogervorst, Frank B. L.
A1  - Jonkers, Jos
T1  - A High-Throughput Functional Complementation Assay for Classification of BRCA1 Missense Variants
JF  - Cancer Discovery
Y1  - 2013
U6  - http://dx.doi.org/10.1158/2159-8290.CD-13-0094
SN  - 2159-8290
IS  - 3
SP  - 1142
EP  - 1152
ER  - 
TY  - JOUR
A1  - Scheer, Nico
A1  - Snaith, Mike
A1  - Wolf, C. Roland
A1  - Seibler, Jost
T1  - Generation and utility of genetically humanized mouse models
JF  - Drug Discovery Today
Y1  - 2013
U6  - http://dx.doi.org/10.1016/j.drudis.2013.07.007
SN  - 1359-6446
VL  - Vol 18
IS  - 23-24
SP  - 1200
EP  - 1211
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Breuer, Lars
A1  - Raue, Markus
A1  - Kirschbaum, M.
A1  - Mang, Thomas
A1  - Schöning, Michael Josef
A1  - Thoelen, R.
A1  - Wagner, Torsten
T1  - Light-controllable polymeric material based on temperature-sensitive hydrogels with incorporated graphene oxide
JF  - Physica status solidi (a)
N2  - Poly(N-isopropylacrylamide) (PNIPAAm) hydrogel films with incorporated graphene oxide (GO) were developed and tested as light-stimulated actuators. GO dispersions were synthesized via Hummers method and characterized toward their optical properties and photothermal energy conversion. The hydrogels were prepared by means of photopolymerization. In addition, the influence of GO within the hydrogel network on the lower critical solution temperature (LCST) was investigated by differential scanning calorimetry (DSC). The optical absorbance and the response to illumination were determined as a function of GO concentration for thin hydrogel films. A proof of principle for the stimulation with light was performed.
Y1  - 2015
U6  - http://dx.doi.org/10.1002/pssa.201431944
SN  - 1862-6319
VL  - 212
IS  - 6
SP  - 1368
EP  - 1374
PB  - Wiley
CY  - Weinheim
ER  -