TY - JOUR A1 - Haeger, Gerrit A1 - Jolmes, Tristan A1 - Oyen, Sven A1 - Jaeger, Karl-Erich A1 - Bongaerts, Johannes A1 - Schörken, Ulrich A1 - Siegert, Petra T1 - Novel recombinant aminoacylase from Paraburkholderia monticola capable of N-acyl-amino acid synthesis JF - Applied Microbiology and Biotechnology N2 - N-Acyl-amino acids can act as mild biobased surfactants, which are used, e.g., in baby shampoos. However, their chemical synthesis needs acyl chlorides and does not meet sustainability criteria. Thus, the identification of biocatalysts to develop greener synthesis routes is desirable. We describe a novel aminoacylase from Paraburkholderia monticola DSM 100849 (PmAcy) which was identified, cloned, and evaluated for its N-acyl-amino acid synthesis potential. Soluble protein was obtained by expression in lactose autoinduction medium and co-expression of molecular chaperones GroEL/S. Strep-tag affinity purification enriched the enzyme 16-fold and yielded 15 mg pure enzyme from 100 mL of culture. Biochemical characterization revealed that PmAcy possesses beneficial traits for industrial application like high temperature and pH-stability. A heat activation of PmAcy was observed upon incubation at temperatures up to 80 °C. Hydrolytic activity of PmAcy was detected with several N-acyl-amino acids as substrates and exhibited the highest conversion rate of 773 U/mg with N-lauroyl-L-alanine at 75 °C. The enzyme preferred long-chain acyl-amino-acids and displayed hardly any activity with acetyl-amino acids. PmAcy was also capable of N-acyl-amino acid synthesis with good conversion rates. The best synthesis results were obtained with the cationic L-amino acids L-arginine and L-lysine as well as with L-leucine and L-phenylalanine. Exemplarily, L-phenylalanine was acylated with fatty acids of chain lengths from C8 to C18 with conversion rates of up to 75%. N-lauroyl-L-phenylalanine was purified by precipitation, and the structure of the reaction product was verified by LC–MS and NMR. KW - Chaperone KW - Biocatalysis KW - Aminoacylase KW - Acylation KW - Acyl-amino acids KW - Biosurfactants Y1 - 2024 U6 - http://dx.doi.org/10.1007/s00253-023-12868-8 SN - 1432-0614 N1 - Corresponding author: Petra Siegert IS - 108 PB - Springer CY - Berlin ER - TY - BOOK A1 - Scheilen, Jana A1 - Peiffer, Max A1 - Görlich, Anna-Luisa A1 - Hacke, Theresa ED - Kroll-Ludwigs, Kathrin ED - Bassen-Metz, Yasmine ED - Bernecker, Andreas ED - Eggert, Mathias ED - Fritz, Thomas ED - Golland, Alexander ED - Höhne, Tim ED - Tran, Duc Hung ED - Vogt, Jürgen T1 - Aachener Online-Schriften Wirtschaft und Recht. Band 7. 02/2023 N2 - 1. Die Wirksamkeitsvoraussetzungen der Verdachtskündigung gegenüber einem Arbeitnehmer - Jana Scheilen | Seite 4-66 2. Besteuerungsinkongruenzen im Internationalen Steuerrecht - die Umsetzung der ATAD II in Form des § 4k EStG in Bezug auf hybride Gesellschaften und Betriebsstätten - Max Peiffer | Seite 67-126 3. Der Zuzug einer Gesellschaft aus einem Drittstaat nach Deutschland - Herausforderungen durch die modifizierte Sitztheorie und Lösungsmöglichkeiten - Anna-Luisa Görlich | Seite 127-189 4. Escalation of Commitment und seine Einflussfaktoren in der unternehmerischen Entscheidungsfindung: Ein Szenario-Experiment - Theresa Hacke | Seite 190-245 T3 - Aachener Online-Schriften Wirtschaft und Recht - 7 Y1 - 2023 PB - FH Aachen / Fachbereich Wirtschaftswissenschaften CY - Aachen ER - TY - JOUR A1 - Hammer, Thorben A1 - Quitter, Julius A1 - Mayntz, Joscha A1 - Bauschat, J.-Michael A1 - Dahmann, Peter A1 - Götten, Falk A1 - Hille, S. A1 - Stumpf, E. T1 - Free fall drag estimation of small-scale multirotor unmanned aircraft systems using computational fluid dynamics and wind tunnel experiments JF - CEAS Aeronautical Journal N2 - New European Union (EU) regulations for UAS operations require an operational risk analysis, which includes an estimation of the potential danger of the UAS crashing. A key parameter for the potential ground risk is the kinetic impact energy of the UAS. The kinetic energy depends on the impact velocity of the UAS and, therefore, on the aerodynamic drag and the weight during free fall. Hence, estimating the impact energy of a UAS requires an accurate drag estimation of the UAS in that state. The paper at hand presents the aerodynamic drag estimation of small-scale multirotor UAS. Multirotor UAS of various sizes and configurations were analysed with a fully unsteady Reynolds-averaged Navier–Stokes approach. These simulations included different velocities and various fuselage pitch angles of the UAS. The results were compared against force measurements performed in a subsonic wind tunnel and provided good consistency. Furthermore, the influence of the UAS`s fuselage pitch angle as well as the influence of fixed and free spinning propellers on the aerodynamic drag was analysed. Free spinning propellers may increase the drag by up to 110%, depending on the fuselage pitch angle. Increasing the fuselage pitch angle of the UAS lowers the drag by 40% up to 85%, depending on the UAS. The data presented in this paper allow for increased accuracy of ground risk assessments. KW - Multirotor UAS KW - Drag estimation KW - CFD KW - Wind tunnel experiments KW - Wind milling Y1 - 2023 U6 - http://dx.doi.org/10.1007/s13272-023-00702-w SN - 1869-5590 (Online) SN - 1869-5582 (Print) N1 - Corresponding author: Thorben Hammer PB - Springer CY - Wien ER - TY - JOUR A1 - Richter, Charlotte A1 - Braunstein, Björn A1 - Stäudle, Benjamin A1 - Attias, Julia A1 - Süss, Alexander A1 - Weber, Tobias A1 - Mileva, Katya N. A1 - Rittweger, Jörn A1 - Green, David A. A1 - Albracht, Kirsten T1 - Gastrocnemius medialis contractile behavior during running differs between simulated Lunar and Martian gravities JF - Scientific reports N2 - The international partnership of space agencies has agreed to proceed forward to the Moon sustainably. Activities on the Lunar surface (0.16 g) will allow crewmembers to advance the exploration skills needed when expanding human presence to Mars (0.38 g). Whilst data from actual hypogravity activities are limited to the Apollo missions, simulation studies have indicated that ground reaction forces, mechanical work, muscle activation, and joint angles decrease with declining gravity level. However, these alterations in locomotion biomechanics do not necessarily scale to the gravity level, the reduction in gastrocnemius medialis activation even appears to level off around 0.2 g, while muscle activation pattern remains similar. Thus, it is difficult to predict whether gastrocnemius medialis contractile behavior during running on Moon will basically be the same as on Mars. Therefore, this study investigated lower limb joint kinematics and gastrocnemius medialis behavior during running at 1 g, simulated Martian gravity, and simulated Lunar gravity on the vertical treadmill facility. The results indicate that hypogravity-induced alterations in joint kinematics and contractile behavior still persist between simulated running on the Moon and Mars. This contrasts with the concept of a ceiling effect and should be carefully considered when evaluating exercise prescriptions and the transferability of locomotion practiced in Lunar gravity to Martian gravity. KW - Bone quality and biomechanics KW - Environmental impact KW - Skeletal muscle KW - Tendons KW - Ultrasound Y1 - 2021 U6 - http://dx.doi.org/10.1038/s41598-021-00527-9 SN - 2045-2322 N1 - Corresponding author: Charlotte Richter VL - 11 IS - Article number: 22555 PB - Springer Nature CY - London ER - TY - GEN T1 - Ordnung zur Änderung der Prüfungsordnung für die Bachelorstudiengänge „Schienenfahrzeugtechnik“ „Schienenfahrzeugtechnik (Teilzeit)“ und „Dualer Studiengang Schienenfahrzeugtechnik (DIRail)“ im Fachbereich Maschinenbau und Mechatronik an der Fachhochschule Aachen T3 - FH-Mitteilungen - 70/2019 KW - Amtliche Mitteilung KW - Bachelor KW - Prüfungsordnung KW - Änderungsordnung KW - Maschinenbau und Mechatronik Y1 - 2019 ER - TY - GEN T1 - Prüfungsordnung für die Bachelorstudiengänge „Schienenfahrzeugtechnik“ „Schienenfahrzeugtechnik (Teilzeit)“ und „Dualer Studiengang Schienenfahrzeugtechnik (DIRail)“ im Fachbereich Maschinenbau und Mechatronik an der Fachhochschule Aachen T3 - FH-Mitteilungen - 71/2019 KW - Prüfungsordnung KW - Bachelor KW - Maschinenbau und Mechatronik KW - lesbare Fassung Y1 - 2019 ER - TY - GEN T1 - Ordnung zur Änderung der Ordnung zur Aufhebung der Studien- und Prüfungsordnung für den Masterstudiengang „Bauingenieurwesen“ vom 10. Dezember 2012 (FH-Mitteilung Nr. 129/2012) in der Fassung der Bekanntmachung der Änderungsordnungen vom 30. Mai 2017 (FH-Mitteilung Nr. 58/2017) an der Fachhochschule Aachen T3 - FH-Mitteilungen - 81/2019 KW - Amtliche Mitteilung KW - Änderungsordnung KW - Studien- und Prüfungsordnung KW - Master KW - Bauingenieurwesen Y1 - 2019 ER - TY - GEN T1 - Ordnung zur Änderung der Ordnung zur Aufhebung der Studien- und Prüfungsordnung für den Masterstudiengang „Facility Management“ vom 29. November 2012 (FH-Mitteilung Nr. 127/2012) in der Fassung der Bekanntmachung der Änderungsordnungen vom 3. Februar 2016 (FH-Mitteilung Nr. 10/2016) an der Fachhochschule Aachen T3 - FH-Mitteilungen - 83/2019 KW - Amtliche Mitteilung KW - Änderungsordnung KW - Studien- und Prüfungsordnung KW - Master KW - Facility Management Y1 - 2019 ER - TY - GEN T1 - Studien- und Prüfungsordnung für den Bachelorstudiengang Bauingenieurwesen (7-semestrig), Bachelorstudiengang Bauingenieurwesen mit Praxissemester (8-semestrig) und Bachelorstudiengang Bauingenieurwesen mit Auslandssemester (8-semestrig) Abschluss Bachelor of Engineering T3 - FH-Mitteilungen - 84/2019 KW - Amtliche Mitteilung KW - Lesbare Fassung KW - Studien- und Prüfungsordnung KW - Bachelor KW - Bauingenieurwesen Y1 - 2019 ER - TY - GEN T1 - Studien- und Prüfungsordnung für den Bachelorstudiengang Bauingenieurwesen (7-semestrig), Bachelorstudiengang Bauingenieurwesen mit Praxissemester (8-semestrig), Bachelorstudiengang Bauingenieurwesen mit Auslandssemester (8-semestrig) Abschluss Bachelor of Engineering : vom 1. September 2011 – FH-Mitteilung Nr. 76/2011 in der Fassung der Bekanntmachung der Änderungsordnung vom 16. August 2019 – FH-Mitteilung Nr. 74/2019 (Nichtamtliche lesbare Fassung, Studienbeginn WS 2016/17–2017/18) T3 - FH-Mitteilungen - 85/2019 KW - Amtliche Mitteilung KW - Studien- und Prüfungsordnung KW - Lesbare Fassung KW - Bachelor KW - Bauingenieurwesen Y1 - 2019 ER -