TY - JOUR A1 - Wu, Chunsheng A1 - Bronder, Thomas A1 - Poghossian, Arshak A1 - Werner, Frederik A1 - Bäcker, Matthias A1 - Schöning, Michael Josef T1 - Label-free electrical detection of DNA with a multi-spot LAPS: First step towards light-addressable DNA chips JF - Physica status solidi A : Applications and materials science N2 - A multi-spot (4 × 4 spots) light-addressable potentiometric sensor (MLAPS) consisting of an Al–p-Si–SiO2 structure has been applied for the label-free electrical detection of DNA (deoxyribonucleic acid) immobilization and hybridization by the intrinsic molecular charge for the first time. Single-stranded probe ssDNA molecules (20 bases) were covalently immobilized onto the silanized SiO2 gate surface. The unspecific adsorption of mismatch ssDNA on the MLAPS gate surface was blocked by bovine serum albumin molecules. To reduce the screening effect and to achieve a high sensor signal, the measurements were performed in a low ionic-strength solution. The photocurrent–voltage (I–V) curves were simultaneously recorded on all 16 spots after each surface functionalization step. Large shifts of I–V curves of 25 mV were registered after the DNA immobilization and hybridization event. In contrast, a small potential shift (∼5 mV) was observed in case of mismatch ssDNA, revealing good specificity of the sensor. The obtained results demonstrate the potential of the MLAPS as promising transducer platform for the multi-spot label-free electrical detection of DNA molecules by their intrinsic molecular charge. Y1 - 2014 U6 - https://doi.org/10.1002/pssa.201330442 SN - 1521-396X (E-Journal); 1862-6319 (E-Journal); 0031-8965 (Print); 1862-6300 (Print) VL - 211 IS - 6 SP - 1423 EP - 1428 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Wrede, Oliver T1 - Mnemotechnik in graphischen Benutzeroberflächen JF - Form / Form-Diskurs : Zeitschrift für Design und Theorie. 1 (1996), H. 2 Y1 - 1996 ER - TY - JOUR A1 - Wrede, Oliver T1 - Continuity in travel information JF - Information Design Journal N2 - This article discusses the contrast between the information transportation companies provide to travellers and that of their brand messaging. Companies’ brand messaging often portrays the service they provide as pleasant, stress free and perfect. Customers and users of the service, on the other hand, often describe their experience of the service as a negative one. This article suggests that the brand value would be greater if transportation companies paid more attention to the users’ experience when designing their information systems, particularly in worst case scenarios. Y1 - 2016 U6 - https://doi.org/10.1075/idj.22.2.09wre SN - 0142-5471 N1 - Gedruckt vorhanden in der Bereichsbibliothek Boxgraben unter 53 Z 971-2016 (Magazin) VL - 22 IS - 2 SP - 172 EP - 178 PB - John Benjamins CY - Amsterdam ER - TY - JOUR A1 - Wollbrink, Moritz A1 - Maslo, Semir A1 - Zimmer, Daniel A1 - Abbas, Karim A1 - Arntz, Kristian A1 - Bergs, Thomas T1 - Clamping and substrate plate system for continuous additive build-up and post-processing of metal parts JF - Procedia CIRP N2 - The manufacturing share of laser powder bed fusion (L-PBF) increases in industrial application, but still many process steps are manually operated. Additionally, it is not possible to achieve tight dimensional tolerances or low surfaces roughness. Hence, a process chain has to be set up to combine additive manufacturing (AM) with further machining technologies. To achieve a continuous workpiece flow as basis for further industrialization of L-PBF, the paper presents a novel substrate system and its application on L-PBF machines and post-processing. The substrate system consists of a zero-point clamping system and a matrix-like interface of contact pins to be substantially connected to the workpiece within the L-PBF process. Y1 - 2020 U6 - https://doi.org/10.1016/j.procir.2020.04.015 SN - 2212-8271 VL - 93 SP - 108 EP - 113 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Wolisz, Henryk A1 - Schütz, Thomas A1 - Blanke, Tobias A1 - Hagenkamp, Markus A1 - Kohrn, Markus A1 - Wesseling, Mark A1 - Müller, Dirk T1 - Cost optimal sizing of smart buildings' energy system components considering changing end-consumer electricity markets JF - Energy Y1 - 2017 U6 - https://doi.org/10.1016/j.energy.2017.06.025 VL - 137 SP - 715 EP - 728 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Wolf, Martin R. A1 - Simon, S. A1 - Schlick, Christopher T1 - Qualitative and Quantitative Analysis of Cooperation / Wolf, M. ; Simon, S. ; Schlick, C. JF - Proceedings : May 26 - 29, 1998, Cannes (France) = Actes : 26 - 29 mai 1998 / INRIA, Institut National de Recherche en Informatique et en Automatique, Unité de recherche ... F. Darses ..., eds Y1 - 1998 N1 - COOP ; Institut National de Recherche en Informatique et en Automatique ; Centre ; (Sophia Antipolis) ; International Conference on the Design of Cooperative Systems ; (3 ; 1998.05.26-29 ; Cannes) ; Conference Internationale sur la Conception de Systèmes Coopératifs ; (3 ; 1998.05.26-29 ; Cannes) ; COOP ; (3 ; 1998.05.26-29 ; Cannes) SP - 1 EP - 5 PB - - CY - Cannes ER - TY - JOUR A1 - Wolf, Martin R. A1 - Schlick, Christopher A1 - Springer, J. ED - Miyamoto, Hiroyuki T1 - The use of implicit communication mechanisms in desktop-teleconferencing-systems JF - Proceedings of WWDU '97 Tokyo, Fifth International Scientific Conference on Work with Display Units : November 3 - 5, 1997, Tokyo Y1 - 1997 N1 - International Scientific Conference on Work with Display Units ; International Scientific Conference on Work with Display Units ; (5 ; 1997.11.03-05 ; Tokyo) SP - 193 EP - 194 PB - - CY - Tokyo ER - TY - JOUR A1 - Wolf, Martin R. A1 - Luczak, Holger A1 - Springer, J. A1 - Simon, S. ED - Miyamoto, Hiroyuki T1 - The role of metaphors in computer supported cooperative work JF - Proceedings of WWDU '97 Tokyo, Fifth International Scientific Conference on Work with Display Units : November 3 - 5, 1997, Tokyo / Y1 - 1997 N1 - International Scientific Conference on Work with Display Units ; International Scientific Conference on Work with Display Units ; (5 ; 1997.11.03-05 ; Tokyo) SP - 191 EP - 192 PB - - CY - Tokyo ER - TY - JOUR A1 - Wolf, Martin R. A1 - Luczak, Holger T1 - Computer Supported Communication and Cooperation – Making Information Aware / Luczak, H. ; Wolf, M. JF - Human-computer interaction : proceedings of HCI International '99 (the 8th International Conference on Human-Computer Interaction), Munich, Germany, August 22 - 26, 1999 Y1 - 1999 N1 - HCI - International Conference on Human-Computer Interaction ; 8 (Munich, Germany) : 1999.08.22-26 ; in 2 Bänden SP - 298 EP - 302 PB - Erlbaum CY - Mahwah, NJ ER - TY - JOUR A1 - Wolf, Martin R. A1 - Foltz, Christian A1 - Schlick, Christopher A1 - Luczak, Holger T1 - Empirical Investigation of a workspace model for Chemical engineers / Wolf, Martin ; Foltz, Christian ; Schlick, Christopher ; Luczak, Holger JF - Proceedings of the Human Factors and Ergonomics Society Annual Meeting July 2000. 44 (2000), H. 6 Y1 - 2000 N1 - Proceedings of the IEA 2000/HFES 2000 Congress SP - 612 EP - 615 PB - - ER -