TY  - CHAP
A1  - Feldhusen, Jörg
A1  - Brezing, Alexander Nikolaus
A1  - Pütz, Claus
A1  - Wählisch, Georg
T1  - Multi-system CAD-teaching in large classes
T2  - When design education and design research meet : proceedings of the 12th International Conference on Engineering and Product Design Education, Norwegian University of Science and Technology (NTNU) Trondheim, Norway, 2nd - 3rd September 2010 ; [E&PDE]
Y1  - 2010
SN  - 978-1-904670-19-3
SP  - 204
EP  - 209
PB  - Design Society
CY  - Glasgow
ER  - 
TY  - BOOK
A1  - Buyer, Christoph
A1  - Klein, Hartmut
A1  - Müller, Thomas
T1  - Änderung der Unternehmensform :  Handbuch zum Umwandlungs- und Umwandlungssteuerrecht
Y1  - 2010
SN  - 978-3-482-42258-4
PB  - Verl. Neue Wirtschafts-Briefe
CY  - Herne
ET  - 8. Aufl.
ER  - 
TY  - GEN
A1  - Alt, Helmut
T1  - Vom Studium der Kerntechnik zu Energie zwischen Wunsch und Wirklichkeit : [Vortrag : Verleihung der KTG-Ehrenmitgliedschaft, Berlin, November 2010]
Y1  - 2010
SP  - 1
EP  - 15
ER  - 
TY  - GEN
A1  - Alt, Helmut
T1  - Optionen einer bezahlbaren Energieversorgung „Vision versus Realismus" : Beitrag für KTG Kongress: vom 4. bis 6. Mai 2010, Berlin
Y1  - 2010
SP  - 1
EP  - 10
ER  - 
TY  - CHAP
A1  - Gligorevic, Snjezana
A1  - Pulini, Paola
T1  - Simplified airport surface channel model based on the WSSUS assumption
T2  - 2010 Integrated Communications, Navigation and Surveillance Conference (ICNS 2010) : Herndon, Virginia, USA, 11 - 13 May 2010
Y1  - 2010
SN  - 978-1-4244-7457-8
SN  - 2155-4943
SP  - F2-1
EP  - F2-11
ER  - 
TY  - CHAP
A1  - Schnell, Michael
A1  - Franzen, Nico
A1  - Gligorevic, Snjezana
T1  - L-DACS1 laboratory demonstrator development and compatibility measurement set-up
T2  - IEEE/AIAA 29th Digital Avionics Systems Conference (DASC) : 3 - 7 Oct. 2010, Salt Lake City, Utah
Y1  - 2010
SN  - 9781424466160 ; 9781424466184
SP  - 3E3-1
EP  - 3E3-11
ER  - 
TY  - BOOK
A1  - Thomas, Axel
T1  - Rhetorische Knallfrösche : Zitatensammlung für Politik und Wirtschaft
Y1  - 2010
SN  - 978-3-00-033037-7
PB  - Neck + Heyn
CY  - Aachen
ER  - 
TY  - CHAP
A1  - Walter, Michael
A1  - Gligorevic, Snjezana
A1  - Detert, Thorben
A1  - Schnell, Michael
T1  - UHF/VHF air-to-air propagation measurements
T2  - Proceedings of the Fourth European Conference on Antennas and Propagation (EuCAP) : 12 - 16 April 2010, Barcelona, Spain
Y1  - 2010
SN  - 978-1-4244-6431-9 ; 978-84-7653-472-4
SP  - 1
EP  - 5
ER  - 
TY  - JOUR
A1  - Ribitsch, D.
A1  - Karl, W.
A1  - Birner-Gruenberger, R.
A1  - Gruber, K.
A1  - Eiteljoerg, I.
A1  - Remler, P.
A1  - Wieland, S.
A1  - Siegert, Petra
A1  - Maurer, Karl-Heinz
A1  - Schwab, H.
T1  - C-terminal truncation of a metagenome-derived detergent protease for effective expression in E. coli
JF  - Journal of biotechnology
N2  - Recently, a new alkaline protease named HP70 showing highest homology to extracellular serine proteases of Stenotrophomonas maltophilia and Xanthomonas campestris was found in the course of a metagenome screening for detergent proteases (Niehaus et al., submitted for publication). Attempts to efficiently express the enzyme in common expression hosts had failed. This study reports on the realization of overexpression in Escherichia coli after structural modification of HP70. Modelling of HP70 resulted in a two-domain structure, comprising the catalytic domain and a C-terminal domain which includes about 100 amino acids. On the basis of the modelled structure the enzyme was truncated by deletion of most of the C-terminal domain yielding HP70-C477.

This structural modification allowed effective expression of active enzyme using E. coli BL21-Gold as the host. Specific activity of HP70-C477 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 30 ± 5 U/mg compared to 8 ± 1 U/mg of the native enzyme. HP70-C477 was most active at 40 °C and pH 7–11; these conditions are prerequisite for a potential application as detergent enzyme. Determination of kinetic parameters at 40 °C and pH = 9.5 resulted in KM = 0.23 ± 0.01 mM and kcat = 167.5 ± 3.6 s⁻¹. MS-analysis of peptide fragments obtained from incubation of HP70 and HP70-C477 with insulin B indicated that the C-terminal domain influences the cleavage preferences of the enzyme. Washing experiments confirmed the high potential of HP70-C477 as detergent protease.
Y1  - 2010
U6  - http://dx.doi.org/10.1016/j.jbiotec.2010.09.947
SN  - 1873-4863 (E-Journal); 0168-1656 (Print)
VL  - 150
IS  - 3
SP  - 408
EP  - 416
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - PAT
A1  - Siegert, Petra
A1  - Mussmann, Nina
A1  - O'Connell, Timothy
A1  - Maurer, Karl-Heinz
T1  - Neue Proteasen und Mittel enthaltend diese Proteasen [Offenlegungsschrift]
T1  - Novel proteases and means containing said proteases [Internationale Patentanmeldung]
Y1  - 2010
SP  - 1
EP  - 30
PB  - Deutsches Patent- und Markenamt / WIPO
CY  - München / Genf
ER  -