TY - JOUR A1 - Jablonski, Melanie A1 - Poghossian, Arshak A1 - Severin, Robin A1 - Keusgen, Michael A1 - Wege, Christian A1 - Schöning, Michael Josef T1 - Capacitive Field-Effect Biosensor Studying Adsorption of Tobacco Mosaic Virus Particles JF - Micromachines N2 - Plant virus-like particles, and in particular, tobacco mosaic virus (TMV) particles, are increasingly being used in nano- and biotechnology as well as for biochemical sensing purposes as nanoscaffolds for the high-density immobilization of receptor molecules. The sensitive parameters of TMV-assisted biosensors depend, among others, on the density of adsorbed TMV particles on the sensor surface, which is affected by both the adsorption conditions and surface properties of the sensor. In this work, Ta₂O₅-gate field-effect capacitive sensors have been applied for the label-free electrical detection of TMV adsorption. The impact of the TMV concentration on both the sensor signal and the density of TMV particles adsorbed onto the Ta₂O₅-gate surface has been studied systematically by means of field-effect and scanning electron microscopy methods. In addition, the surface density of TMV particles loaded under different incubation times has been investigated. Finally, the field-effect sensor also demonstrates the label-free detection of penicillinase immobilization as model bioreceptor on TMV particles. KW - capacitive field-effect sensor KW - plant virus detection KW - tobacco mosaic virus (TMV) KW - TMV adsorption KW - Ta₂O₅ gate Y1 - 2021 U6 - http://dx.doi.org/10.3390/mi12010057 VL - 12 IS - 1 PB - MDPI CY - Basel ER - TY - JOUR A1 - Bäcker, Matthias A1 - Poghossian, Arshak A1 - Abouzar, Maryam H. A1 - Wenmackers, Sylvia A1 - Janssens, Stoffel D. A1 - Haenen, Ken A1 - Wagner, Patrick A1 - Schöning, Michael Josef T1 - Capacitive field-effect (bio-)chemical sensors based on nanocrystalline diamond films JF - Diamond Electronics and Bioelectronics — Fundamentals to Applications III, edited by P. Bergonzo, [u.a.] Y1 - 2010 N1 - MRS Proceedings Volume 1203 paper 1203-J17-31 ; Mater. Res. Soc. Sympos. Proc. Vol 1203 (2010) ; Materials Research Society SP - 1 EP - 6 ER - TY - JOUR A1 - Abouzar, Maryam H. A1 - Poghossian, Arshak A1 - Siqueira, José R. Jr. A1 - Oliveira, Osvaldo N. Jr. A1 - Moritz, Werner A1 - Schöning, Michael Josef T1 - Capacitive electrolyte-insulator-semiconductor structures functionalised with a polyelectrolyte/enzyme multilayer: New strategy for enhanced field-effect biosensing JF - Physica Status Solidi (A). 207 (2010), H. 4 Y1 - 2010 SN - 1862-6300 N1 - Special Issue: Engineering of Functional Interfaces EnFI 2009 SP - 884 EP - 890 ER - TY - JOUR A1 - Abouzar, Maryam H. A1 - Werner, Moritz A1 - Schöning, Michael Josef A1 - Poghossian, Arshak T1 - Capacitance–voltage and impedance-spectroscopy characteristics of nanoplate EISOI capacitors JF - Physica status solidi (a) : applications and material science. 208 (2011), H. 6 Y1 - 2011 SN - 1862-6319 SP - 1327 EP - 1332 PB - Wiley-VCH CY - Berlin ER - TY - JOUR A1 - Poghossian, Arshak A1 - Abouzar, Maryam H. A1 - Schöning, Michael Josef T1 - Capacitance–voltage and impedance characteristics of field-effect EIS sensors functionalised with polyelectrolyte multilayers JF - IRBM. 29 (2008), H. 2-3 Y1 - 2008 SN - 1959-0318 SP - 149 EP - 154 ER - TY - JOUR A1 - Schöning, Michael Josef A1 - Mourzina, Y. G. A1 - Schubert, J. A1 - Zander, W. A1 - Legin, A. A1 - Vlasov, Y. G. A1 - Lüth, H. T1 - Can pulsed laser deposition serve as an advanced technique in fabricating chemical sensors? JF - Sensors and Actuators B. 78 (2001), H. 1-3 Y1 - 2001 SN - 0925-4005 N1 - Selected Papers form Eurosensors XIV SP - 273 EP - 278 ER - TY - JOUR A1 - Schöning, Michael Josef A1 - Mourzina, Y. G. A1 - Schubert, J. A1 - Zander, W. A1 - Legin, A. A1 - Vlasov, Y. G. A1 - Lüth, H. T1 - Can pulsed laser deposition serve as an advanced technique in fabricating chemical sensors? JF - Proceedings : Copenhagen, Denmark, 27 - 30 August 2000 / [ed.: R. de Reus ...] Y1 - 2000 SN - 87-89935-50-0 N1 - Eurosensors ; (14, 2000, København) ; Eurosensors ; (14 : ; 2000.08.27-30 : ; Copenhagen) ; European Conference on Solid-State Transducers ; (14 : ; 2000.08.27-30 : ; Copenhagen) SP - 615 EP - 618 PB - MIC, Mikroelektronik Centret CY - Lyngby, Denmark ER - TY - JOUR A1 - Paczkowski, Sebastian A1 - Weißbecker, Bernhard A1 - Schöning, Michael Josef A1 - Schütz, Stefan T1 - Biosensors on the Basis of Insect Olfaction JF - Insect biotechnology / Andreas Vilcinskas, ed. Y1 - 2011 SN - 978-90-481-9640-1 N1 - Biologically-inspired system ; 2 SP - 225 EP - 240 PB - Springer CY - Dordrecht [u.a.] ER - TY - JOUR A1 - Keusgen, Michael A1 - Jünger, Martina A1 - Krest, Ingo A1 - Schöning, Michael Josef T1 - Biosensoric detection of the cysteine sulphoxide alliin JF - Sensors and Actuators B. 95 (2003), H. 1-3 Y1 - 2003 SN - 0925-4005 SP - 297 EP - 302 ER - TY - JOUR A1 - Keusgen, M. A1 - Jünger, M. A1 - Schöning, Michael Josef T1 - Biosensoric detection of the cysteine sulphoxide alliin JF - Book of abstracts / ed. by J. Saneistr. Y1 - 2002 SN - 80-01-02576-4 N1 - Eurosensors ; (16, 2002, Praha) SP - 1175 EP - 1178 PB - Czech Technical University, Faculty of Electrical Engineering, Department of Measurement CY - Prague ER - TY - JOUR A1 - Oliveira, Danilo A. A1 - Molinnus, Denise A1 - Beging, Stefan A1 - Siqueira Jr, José R. A1 - Schöning, Michael Josef T1 - Biosensor Based on Self-Assembled Films of Graphene Oxide and Polyaniline Using a Field-Effect Device Platform JF - physica status solidi (a) applications and materials science N2 - A new functionalization method to modify capacitive electrolyte–insulator–semiconductor (EIS) structures with nanofilms is presented. Layers of polyallylamine hydrochloride (PAH) and graphene oxide (GO) with the compound polyaniline:poly(2-acrylamido-2-methyl-1-propanesulfonic acid) (PANI:PAAMPSA) are deposited onto a p-Si/SiO2 chip using the layer-by-layer technique (LbL). Two different enzymes (urease and penicillinase) are separately immobilized on top of a five-bilayer stack of the PAH:GO/PANI:PAAMPSA-modified EIS chip, forming a biosensor for detection of urea and penicillin, respectively. Electrochemical characterization is performed by constant capacitance (ConCap) measurements, and the film morphology is characterized by atomic force microscopy (AFM) and scanning electron microscopy (SEM). An increase in the average sensitivity of the modified biosensors (EIS–nanofilm–enzyme) of around 15% is found in relation to sensors, only carrying the enzyme but without the nanofilm (EIS–enzyme). In this sense, the nanofilm acts as a stable bioreceptor onto the EIS chip improving the output signal in terms of sensitivity and stability. KW - capacitive electrolyte–insulator–semiconductor sensors KW - graphene oxide KW - layer-by-layer technique KW - nanomaterials KW - polyaniline Y1 - 2021 U6 - http://dx.doi.org/10.1002/pssa.202000747 SN - 1862-6319 N1 - Corresponding author: José R. Siqueira Jr & Michael J. Schöning VL - 218 IS - 13 SP - 1 EP - 9 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Schöning, Michael Josef A1 - Poghossian, Arshak T1 - BioFEDs (field-effect devices) : State-of-the-art and new directions JF - Electroanalysis Y1 - 2006 U6 - http://dx.doi.org/10.1002/elan.200603609 SN - 1521-4109 VL - 18 IS - 19-20 SP - 1893 EP - 1900 ER - TY - JOUR A1 - Schroth, P. A1 - Weißbecker, B. A1 - Schütz, S. A1 - Ecken, H. A1 - Yoshinobu, T. A1 - Lüth, H. A1 - Schöning, Michael Josef T1 - Bioelectronic signal processing - intact chemoreceptors coupled to field-effect devices JF - Lecture Notes of the ICB Seminars Y1 - 2002 SP - 28 EP - 42 PB - MCB CY - Warsaw ER - TY - JOUR A1 - Schroth, P. A1 - Weißbecker, B. A1 - Schütz, S. A1 - Ecken, H. A1 - Yoshinobu, T. A1 - Lüth, H. A1 - Schöning, Michael Josef T1 - Bioelectronic signal processing - intact chemoreceptors coupled to field-effect devices JF - Biocybernetics and Biomedical Engineering. 21 (2001), H. 3 Y1 - 2001 SN - 0208-5216 SP - 27 EP - 42 ER - TY - JOUR A1 - Lüth, H. A1 - Thust, M. A1 - Steffen, A. A1 - Kordos, P. A1 - Schöning, Michael Josef T1 - Biochemical sensors with structured and porous silicon capacitors JF - Materials Science and Engineering B. 69-70 (2000) Y1 - 2000 SN - 0921-5107 SP - 104 EP - 108 ER - TY - JOUR A1 - Kramer, Friederike A1 - Halamkova, Lenka A1 - Poghossian, Arshak A1 - Schöning, Michael Josef A1 - Katz, Evgeny A1 - Halamek, Jan T1 - Biocatalytic analysis of biomarkers for forensic identification of ethnicity between Caucasian and African American JF - The analyst. August 2013 Y1 - 2013 SN - 1364-5528 (E-Journal); 0003-2654 (Print) VL - Vol. 138 SP - 6251 EP - 6257 PB - Royal Society of Chemistry CY - Cambridge ER - TY - JOUR A1 - Buniatyan, V. A1 - Huck, Christina A1 - Poghossian, Arshak A1 - Aroutiounian, V. M. A1 - Schöning, Michael Josef T1 - BaxSr1-x TiO3/pc-Si heterojunction capacitance JF - Armenian journal of physics Y1 - 2013 SN - 1829-1171 VL - 6 IS - 4 SP - 188 EP - 197 PB - National Academy of Sciences of Armenia CY - Yerevan ER - TY - JOUR A1 - Buniatyan, V. A1 - Huck, Christina A1 - Poghossian, Arshak A1 - Aroutiounian, V. M. A1 - Schöning, Michael Josef T1 - BaxSr1-x TiO3/pc-Si heterojunction JF - Armenian journal of physics Y1 - 2013 SN - 1829-1171 VL - 6 IS - 4 SP - 177 EP - 187 PB - National Academy of Sciences of Armenia CY - Yerevan ER - TY - JOUR A1 - Siqueira, José R. Jr. A1 - Bäcker, Matthias A1 - Poghossian, Arshak A1 - Zucolotto, Valtencir A1 - Oliveira, Osvaldo N. Jr. A1 - Schöning, Michael Josef T1 - Associating biosensing properties with the morphological structure of multilayers containing carbon nanotubes on field-effect devices JF - Physica status solidi (a). 207 (2010), H. 4 Y1 - 2010 SN - 1862-6300 N1 - Special Issue: Engineering of Functional Interfaces EnFI 2009 SP - 781 EP - 786 ER - TY - JOUR A1 - Schöning, Michael Josef A1 - Turek, M. A1 - Heiden, W. A1 - Riesen, A. A1 - Chhabda, T. A. A1 - Schubert, J. A1 - Krüger, P. A1 - Keusgen, M. T1 - Artificial intelligence/fuzzy logic method for analysis of combined signals from heavy metal chemical sensors JF - Electrochimica Acta. 54 (2009), H. 25 Sp. Iss. SI Y1 - 2009 SN - 0013-4686 SP - 6082 EP - 6088 PB - Elsevier CY - New York ER - TY - JOUR A1 - Figueroa-Miranda, Gabriela A1 - Feng, Lingyan A1 - Shiu, Simon Chi-Chin A1 - Dirkzwager, Roderick Marshall A1 - Cheung, Yee-Wai A1 - Tanner, Julian Alexander A1 - Schöning, Michael Josef A1 - Offenhäusser, Andreas A1 - Mayer, Dirk T1 - Aptamer-based electrochemical biosensor for highly sensitive and selective malaria detection with adjustable dynamic response range and reusability JF - Sensor and Actuators B: Chemical N2 - Malaria infection remains a significant risk for much of the population of tropical and subtropical areas, particularly in developing countries. Therefore, it is of high importance to develop sensitive, accurate and inexpensive malaria diagnosis tests. Here, we present a novel aptamer-based electrochemical biosensor (aptasensor) for malaria detection by impedance spectroscopy, through the specific recognition between a highly discriminatory DNA aptamer and its target Plasmodium falciparum lactate dehydrogenase (PfLDH). Interestingly, due to the isoelectric point (pI) of PfLDH, the aptasensor response showed an adjustable detection range based on the different protein net-charge at variable pH environments. The specific aptamer recognition allows sensitive protein detection with an expanded detection range and a low detection limit, as well as a high specificity for PfLDH compared to analogous proteins. The specific feasibility of the aptasensor is further demonstrated by detection of the target PfLDH in human serum. Furthermore, the aptasensor can be easily regenerated and thus applied for multiple usages. The robustness, sensitivity, and reusability of the presented aptasensor make it a promising candidate for point-of-care diagnostic systems. Y1 - 2018 U6 - http://dx.doi.org/10.1016/j.snb.2017.07.117 SN - 0925-4005 VL - 255 IS - P1 SP - 235 EP - 243 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Yoshinobu, T. A1 - Yasuhito, U. A1 - Iwasaki, H. A1 - Näther, Niko A1 - Schöning, Michael Josef A1 - Koudelka-Hep, M. T1 - Applications of the light-addressable potentiometric sensor to microfluidic devices JF - Digest of technical papers : September 12 - 15, 2004, Rome, Italy, Pontificia Universitas Sancto Thoma Aquinate in Urbe / [conference chairperson: C. Di Natale]. Y1 - 2004 SN - 88-7621-282-5 N1 - Eurosensors ; (18, 2004, Roma) SP - 434 EP - 437 CY - Roma ER - TY - JOUR A1 - Schütz, S. A1 - Hummel, Hans E. A1 - Schöning, Michael Josef A1 - Schroth, P. A1 - Kordos, P. A1 - Lüth, H. A1 - Zimmermann, S. A1 - Schwarz, A. A1 - Kohl, C.-D. A1 - Koczan, D. T1 - Applications and perspectives of biosensors based on insect olfaction JF - Sensor 99 : proceedings / 9th International Fair and Conference for Sensors, Transducers & Systems, May 18 - 20, 1999, Exhibition Centre Nürnberg/Germany Y1 - 1999 N1 - Internationale Messe mit Kongreß für Sensoren, Meßaufnehmer & Systeme ; (9, 1999, Nürnberg) SP - 99 EP - 104 PB - AMA Service CY - Wunstorf ER - TY - JOUR A1 - Yoshinobu, Tatsuo A1 - Moritz, Werner A1 - Finger, Friedhelm A1 - Schöning, Michael Josef ED - Comini, Elisabetta T1 - Application of thin-film amorphous silicon to chemical imaging JF - Nanostructured materials and hybrid composites for gas sensors and biomedical applications : symposium held April 18-20, 2006, San Francisco , California, U.S.A. Y1 - 2006 SN - 9781558998711 N1 - Materials Research Society symposia proceedings; v. 915 IS - Paper 0910-A-20-01 SP - 1 EP - 10 ER - TY - JOUR A1 - Poghossian, Arshak A1 - Baade, A. A1 - Emons, H. A1 - Schöning, Michael Josef T1 - Application of ISFET for pH measurements in rain droplets JF - Sensors and Actuators B. 76 (2001), H. 1-3 Y1 - 2001 SN - 0925-4005 SP - 634 EP - 638 ER - TY - JOUR A1 - Miyamato, Ko-ichiro A1 - Sakakita, Sakura A1 - Wagner, Torsten A1 - Schöning, Michael Josef A1 - Yoshinobu, Tatsuo T1 - Application of chemical imaging sensor to in-situ pH imaging in the vicinity of a corroding metal surface JF - Electrochimica Acta N2 - The chemical imaging sensor was applied to in-situ pH imaging of the solution in the vicinity of a corroding surface of stainless steel under potentiostatic polarization. A test piece of polished stainless steel was placed on the sensing surface leaving a narrow gap filled with artificial seawater and the stainless steel was corroded under polarization. The pH images obtained during polarization showed correspondence between the region of lower pH and the site of corrosion. It was also found that the pH value in the gap became as low as 2 by polarization, which triggered corrosion. Y1 - 2015 U6 - http://dx.doi.org/10.1016/j.electacta.2015.07.184 SN - 0013-4686 VL - 183 SP - 137 EP - 142 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Pilas, Johanna A1 - Yazici, Y. A1 - Selmer, Thorsten A1 - Keusgen, M. A1 - Schöning, Michael Josef T1 - Application of a portable multi-analyte biosensor for organic acid determination in silage JF - Sensors N2 - Multi-analyte biosensors may offer the opportunity to perform cost-effective and rapid analysis with reduced sample volume, as compared to electrochemical biosensing of each analyte individually. This work describes the development of an enzyme-based biosensor system for multi-parametric determination of four different organic acids. The biosensor array comprises five working electrodes for simultaneous sensing of ethanol, formate, d-lactate, and l-lactate, and an integrated counter electrode. Storage stability of the biosensor was evaluated under different conditions (stored at +4 °C in buffer solution and dry at −21 °C, +4 °C, and room temperature) over a period of 140 days. After repeated and regular application, the individual sensing electrodes exhibited the best stability when stored at −21 °C. Furthermore, measurements in silage samples (maize and sugarcane silage) were conducted with the portable biosensor system. Comparison with a conventional photometric technique demonstrated successful employment for rapid monitoring of complex media. Y1 - 2018 U6 - http://dx.doi.org/10.3390/s18051470 SN - 1424-8220 VL - 18 IS - 5 PB - MDPI CY - Basel ER - TY - JOUR A1 - Poghossian, Arshak A1 - Schultze, J. W. A1 - Schöning, Michael Josef T1 - Application of a (bio-)chemical sensor (ISFET) for the detection of physical parameters in liquids JF - Electrochimica Acta. 48 (2003), H. 20-22 Y1 - 2003 SP - 3289 EP - 3297 ER - TY - JOUR A1 - Mourzina, Y. G. A1 - Ermelenko, Y. E. A1 - Yoshinobu, T. A1 - Vlasov, Y. A1 - Iwasaki, H. A1 - Schöning, Michael Josef T1 - Anionselective light-addressable potentiometric sensors (LAPS) for the determination of nitrate and suphate ions JF - Sensors and Actuators B. 91 (2003), H. 1-3 Y1 - 2003 SN - 0925-4005 SP - 32 EP - 38 ER - TY - JOUR A1 - Heiden, W. A1 - Turek, M. A1 - Schöning, Michael Josef T1 - Analysis of chemical sensor data JF - Proceedings of the 4th Russian-German Workshop "Innovation Information Technologies: Theory and practice" : Ufa, Russia, April 8-13, 2011 / eds. Yupsova, Nafisa ... Y1 - 2011 SN - 978-5-4221-0159-7 SP - 76 EP - 81 PB - State Aviation Technical Univ. CY - Ufa ER - TY - JOUR A1 - Murib, Mohammed S. A1 - Tran, Anh Quang A1 - Ceuninck, Ward de A1 - Schöning, Michael Josef A1 - Nesladek, Milos A1 - Serpengüzel, Ali A1 - Wagner, Patrick T1 - Analysis of an optical biosensor based on elastic light scattering from diamond-, glass-, and sapphire microspheres JF - Physica Status Solidi A N2 - Deoxyribonucleic acid (DNA) and protein recognition are now standard tools in biology. In addition, the special optical properties of microsphere resonators expressed by the high quality factor (Q-factor) of whispering gallery modes (WGMs) or morphology dependent resonances (MDRs) have attracted the attention of the biophotonic community. Microsphere-based biosensors are considered as powerful candidates to achieve label-free recognition of single molecules due to the high sensitivity of their WGMs. When the microsphere surface is modified with biomolecules, the effective refractive index and the effective size of the microsphere change resulting in a resonant wavelength shift. The transverse electric (TE) and the transverse magnetic (TM) elastic light scattering intensity of electromagnetic waves at 600 and 1400 nm are numerically calculated for DNA and unspecific binding of proteins to the microsphere surface. The effect of changing the optical properties was studied for diamond (refractive index 2.34), glass (refractive index 1.50), and sapphire (refractive index 1.75) microspheres with a 50 µm radius. The mode spacing, the linewidth of WGMs, and the shift of resonant wavelength due to the change in radius and refractive index, were analyzed by numerical simulations. Preliminary results of unspecific binding of biomolecules are presented. The calculated shift in WGMs can be used for biomolecules detection. Y1 - 2012 U6 - http://dx.doi.org/10.1002/pssa.201100795 SN - 1862-6319 N1 - Special Issue: "Fundamentals and Applications of Diamond" VL - 209 IS - 9 SP - 1804 EP - 1810 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Poghossian, Arshak A1 - Schöning, Michael Josef A1 - Schroth, P. A1 - Simonis, A. A1 - Lüth, H. T1 - An ISFET-based penicillin sensor with high sensitivity, low detection limit and long lifetime JF - Sensors and Actuators B. 76 (2001), H. 1-3 Y1 - 2001 SN - 0925-4005 SP - 519 EP - 526 ER - TY - JOUR A1 - Schütz, S. A1 - Schöning, Michael Josef A1 - Schroth, P. A1 - Weißbecker, B. A1 - Kordos, P. A1 - Lüth, H. A1 - Hummel, Hans E. T1 - An insectbased BioFET as a bioelectronic nose JF - Sensors and Actuators B. 65 (2000), H. 1-3 Y1 - 2000 SN - 0925-4005 SP - 291 EP - 295 ER - TY - JOUR A1 - Schütz, S. A1 - Schöning, Michael Josef A1 - Schroth, P. A1 - Weißbecker, B. A1 - Kordos, P. A1 - Lüth, H. A1 - Hummel, Hans E. T1 - An insectbased BioFET as a bioelectronic nose JF - Proceedings of the Seventh International Meeting on Chemical Sensors : 27 - 30 July, 1998, Beijing, P. R. China / ed. Zhi-Gang Zhou Y1 - 1998 N1 - International Meeting on Chemical Sensors ; (7, 1998, Beijing) ; International Meeting on Chemical Sensors (IMCS) ; (7 : ; 1998.07.27-30 : ; Beijing) SP - 314 EP - 316 PB - Elsevier CY - Amsterdam [u.a.] ER - TY - JOUR A1 - Honarvarfard, Elham A1 - Gamella, Maria A1 - Poghossian, Arshak A1 - Schöning, Michael Josef A1 - Katz, Evgeny T1 - An enzyme-based reversible Controlled NOT (CNOT) logic gate operating on a semiconductor transducer JF - Applied Materials Today N2 - An enzyme-based biocatalytic system mimicking operation of a logically reversible Controlled NOT (CNOT) gate has been interfaced with semiconductor electronic transducers. Electrolyte–insulator–semiconductor (EIS) structures have been used to transduce chemical changes produced by the enzyme system to an electronically readable capacitive output signal using field-effect features of the EIS device. Two enzymes, urease and esterase, were immobilized on the insulating interface of EIS structure producing local pH changes performing XOR logic operation controlled by various combinations of the input signals represented by urea and ethyl butyrate. Another EIS transducer was functionalized with esterase only, thus performing Identity (ID) logic operation for the ethyl butyrate input. Both semiconductor devices assembled in parallel operated as a logically reversible CNOT gate. The present system, despite its simplicity, demonstrated for the first time logically reversible function of the enzyme system transduced electronically with the semiconductor devices. The biomolecular realization of a CNOT gate interfaced with semiconductors is promising for integration into complex biomolecular networks and future biosensor/biomedical applications. KW - Electrolyte–insulator–semiconductor KW - Capacitive field-effect KW - CNOT KW - XOR KW - Enzyme logic gate Y1 - 2017 U6 - http://dx.doi.org/10.1016/j.apmt.2017.08.003 SN - 2352-9407 VL - 9 SP - 266 EP - 270 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Moseley, Fiona A1 - Halamek, Jan A1 - Kramer, Friederike A1 - Poghossian, Arshak A1 - Schöning, Michael Josef A1 - Katz, Evgeny T1 - An enzyme-based reversible CNOT logic gate realized in a flow system JF - Analyst N2 - An enzyme system organized in a flow device was used to mimic a reversible Controlled NOT (CNOT) gate with two input and two output signals. Reversible conversion of NAD⁺ and NADH cofactors was used to perform a XOR logic operation, while biocatalytic hydrolysis of p-nitrophenyl phosphate resulted in an Identity operation working in parallel. The first biomolecular realization of a CNOT gate is promising for integration into complex biomolecular networks and future biosensor/biomedical applications. Y1 - 2014 U6 - http://dx.doi.org/10.1039/C4AN00133H SN - 1364-5528 (E-Journal) ; 0003-2654 (Print) VL - 139 IS - 8 SP - 1839 EP - 1842 PB - Royal Society of Chemistry CY - Cambridge ER - TY - JOUR A1 - Abouzar, Maryam H. A1 - Poghossian, Arshak A1 - Pedraza, A. M. A1 - Gandhi, D. A1 - Ingebrandt, S. A1 - Moritz, W. A1 - Schöning, Michael Josef T1 - An array of field-effect nanoplate SOI capacitors for (bio-)chemical sensing JF - Biosensors and Bioelectronics. 26 (2011), H. 6 Y1 - 2011 SN - 0956-5663 SP - 3023 EP - 3028 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Schusser, Sebastian A1 - Poghossian, Arshak A1 - Bäcker, Matthias A1 - Krischer, M. A1 - Leinhos, Marcel A1 - Wagner, P. A1 - Schöning, Michael Josef T1 - An application of field-effect sensors for in-situ monitoring of degradation of biopolymers JF - Sensors and actuators B: Chemical N2 - The characterization of the degradation kinetics of biodegradable polymers is mandatory with regard to their proper application. In the present work, polymer-modified electrolyte–insulator–semiconductor (PMEIS) field-effect sensors have been applied for in-situ monitoring of the pH-dependent degradation kinetics of the commercially available biopolymer poly(d,l-lactic acid) (PDLLA) in buffer solutions from pH 3 to pH 13. PDLLA films of 500 nm thickness were deposited on the surface of an Al–p-Si–SiO2–Ta2O5 structure from a polymer solution by means of spin-coating method. The PMEIS sensor is, in principle, capable to detect any changes in bulk, surface and interface properties of the polymer induced by degradation processes. A faster degradation has been observed for PDLLA films exposed to alkaline solutions (pH 9, pH 11 and pH 13). Y1 - 2015 U6 - http://dx.doi.org/10.1016/j.snb.2014.10.058 SN - 1873-3077 (E-Journal); 0925-4005 (Print) VL - 207, Part B SP - 954 EP - 959 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Schöning, Michael Josef A1 - Jacobs, M. A1 - Muck, A. A1 - Knobbe, D.-T. A1 - Wang, J. A1 - Chatrathi, M. A1 - Spillmann, S. T1 - Amperometric PDMS/glass capillary electrophoresis-based biosensor microchip for catechol and dopamin detection JF - Sensors and Actuators B. 108 (2005), H. 1-2 Y1 - 2005 SN - 0925-4005 N1 - Proceedings of the Tenth International Meeting on Chemical Sensors — IMCS - 10 2004 SP - 688 EP - 694 ER - TY - JOUR A1 - Schöning, Michael Josef A1 - Wang, J. A1 - Jacobs, M. A1 - Knobbe, D.-T. A1 - Muck, A. T1 - Amperometric PDMS-glass capillary electrophoresis-based biosensor microchip for catechol and dopamine detection JF - Technical digest of the 10th International Meeting on Chemical Sensors, July 11 - 14, 2004, Tsukuba, Japan / Japan Association of Chemical Sensors Y1 - 2004 N1 - Chemical sensors ; 20.2004 Suppl. B. IMCS ; (10, 2004, Tsukuba) ; International Meeting on Chemical Sensors ; (10 : ; 2004.07.11-14 : ; Tsukuba) SP - 566 EP - 567 PB - Japan Association of Chemical Sensors CY - Fukuoka ER - TY - JOUR A1 - Yoshinobu, T. A1 - Ecken, H. A1 - Poghossian, Arshak A1 - Lüth, H. A1 - Iwasaki, H. A1 - Schöning, Michael Josef T1 - Alternative sensor materials for light-addressable potentiometric sensors JF - Sensors and Actuators B. 76 (2001), H. 1-3 Y1 - 2001 SN - 0925-4005 SP - 388 EP - 392 ER - TY - JOUR A1 - Bohrn, U. A1 - Stütz, E. A1 - Fuchs, K. A1 - Fleischer, M. A1 - Schöning, Michael Josef A1 - Wagner, P. T1 - Air Quality Monitoring using a Whole-Cell based Sensor System JF - Procedia Engineering. 25 (2011) Y1 - 2011 SN - 1877-7058 N1 - EurosensorsXXV ; Proc. Eurosensors XXV, September 4-7, 2011, Athens, Greece SP - 1421 EP - 1424 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Schöning, Michael Josef A1 - Kloock, Joachim P. T1 - About 20 years of silicon-based thin-film sensors with chalcogenide glass materials for heavy metal analysis: Technological aspects of fabrication and miniaturization JF - Electroanalysis. 19 (2007), H. 19-20 Y1 - 2007 SN - 1040-0397 SP - 2029 EP - 2038 ER - TY - JOUR A1 - Simonis, A. A1 - Krings, T. A1 - Lüth, H. A1 - Wang, J. A1 - Schöning, Michael Josef T1 - A „hybrid“ thin-film pH sensor with integrated thick-film reference JF - Sensors. 1 (2001), H. 6 Y1 - 2001 SN - 1424-8220 SP - 183 EP - 192 ER - TY - JOUR A1 - Schöning, Michael Josef A1 - Weidenbrück, B. A1 - Thust, M. A1 - Kordos, P. A1 - Lüth, H. T1 - A silicon-based sensor array with capacitive EIS structures JF - Proceedings of the 11th European Conference on Solid-State Transducers / Eurosensors XI, September 21 - 24, 1997, Warsaw, Poland. [Organised by] Warsaw University of Technology. Vol 2. Y1 - 1997 SN - 83-908335-0-6 N1 - Eurosensors ; (11, 1997, Warszawa) ; Eurosensors - European Conference on Solid-State Transducers ; (11 : ; 1997.09.21-24 : ; Warsaw) SP - 871 EP - 874 PB - Sensor Lab Sp. CY - Warsaw ER - TY - JOUR A1 - Bäcker, Matthias A1 - Pouyeshman, S. A1 - Schnitzler, Thomas A1 - Poghossian, Arshak A1 - Wagner, Patrick A1 - Biselli, Manfred A1 - Schöning, Michael Josef T1 - A silicon-based multi-sensor chip for monitoring of fermentation processes JF - Physica status solidi (a) : applications and material science. 208 (2011), H. 6 Y1 - 2011 SN - 1862-6319 SP - 1364 EP - 1369 PB - Wiley CY - Weinheim ER - TY - JOUR A1 - Schöning, Michael Josef A1 - Buß, G. A1 - Faßbender, F. A1 - Glück, O. A1 - Schmitt, G. A1 - Schultze, J. W. A1 - Lüth, H. T1 - A silicon-based microelectrode array for chemical analysis JF - Sensors and Actuators B. 65 (2000), H. 1-3 Y1 - 2000 SN - 0925-4005 SP - 284 EP - 287 ER - TY - JOUR A1 - Schöning, Michael Josef A1 - Buß, G. A1 - Faßbender, F. A1 - Glück, O. A1 - Schmitt, G. A1 - Schultze, J. W. A1 - Lüth, H. T1 - A silicon-based microelectrode array for chemical analysis JF - Proceedings of the Seventh International Meeting on Chemical Sensors : 27 - 30 July, 1998, Beijing, P. R. China / ed. Zhi-Gang Zhou Y1 - 1998 N1 - International Meeting on Chemical Sensors ; (7, 1998, Beijing) ; International Meeting on Chemical Sensors (IMCS) ; (7 : ; 1998.07.27-30 : ; Beijing) SP - 100 EP - 102 PB - Elsevier CY - Amsterdam [u.a.] ER - TY - JOUR A1 - Arida, Hassan A1 - Turek, Monika A1 - Rolka, David A1 - Schöning, Michael Josef T1 - A Novel Thin-Film Copper Array Based on an Organic/Inorganic Sensor Hybrid: Microfabrication, Potentiometric Characterization, and Flow-Injection Analysis Application JF - Electroanalysis. 21 (2009), H. 10 Y1 - 2009 SN - 1040-0397 SP - 1145 EP - 1151 PB - Wiley CY - Weinheim ER - TY - JOUR A1 - Schöning, Michael Josef A1 - Thust, M. A1 - Müller-Veggian, Mattea A1 - Kordos, P. T1 - A novel silicon-based sensor array with capacitive EIS structures JF - Sensors and Actuators B. 47 (1998), H. 1-3 Y1 - 1998 SN - 0925-4005 SP - 225 EP - 230 ER - TY - JOUR A1 - Schöning, Michael Josef A1 - Beckers, L. A1 - Schaub, A. A1 - Zander, W. A1 - Schubert, J. A1 - Mesters, S. A1 - Kordos, P. A1 - Lüth, H. T1 - A novel silicon-based pH sensor prepared by pulsed laser deposition technique JF - Proceedings of Eurosensors X, the 10th European Conference on Solid-State Transducers, September 8-11, 1996, Leuven, Belgium / [KU Leuven]. Puers, Robert. Vol. 3. Y1 - 1996 SN - 90-803282-1-9 N1 - Eurosensors ; (10, 1996, Louvain) Eurosensors, the European Conference on Solid-State Transducers ; (10 : ; 1996.09.08-11 : ; Leuven) SP - 781 EP - 784 PB - Catholic University Leuven CY - Heverlee, Belgium ER - TY - JOUR A1 - Näther, Niko A1 - Emmerich, Rüdiger A1 - Berger, Jörg A1 - Friedrich, Peter A1 - Henkel, Hartmut A1 - Schneider, Andreas A1 - Schöning, Michael Josef T1 - A novel gas-phase hydrogen peroxide sensor basing on a combined physical/chemical transduction mechanism JF - Nanofunctional materials, nanostructures, and novel devices for biological and chemical detection : November 27 - December 1, 2006, Boston, Massachusetts, USA ; [at the 2006 MRS Fall Meeting]. Y1 - 2006 SN - 978-1-60423-407-7 N1 - Materials Research Society symposium proceedings ; 951 ; MRS fall meeting ; (2006.11.27-12.01 : ; Boston, Mass.) SP - 63 EP - 68 PB - Materials Research Soc. CY - Warrendale, Pa. ER - TY - JOUR A1 - Mourzina, Y.G. A1 - Schöning, Michael Josef A1 - Schubert, J. A1 - Zander, W. A1 - Legin, A. V. A1 - Vlasov, Y. G. A1 - Kordos, P. A1 - Lüth, H. T1 - A new thin film Pb microsensor based on chalcogenide glasses JF - Sensors and Actuators B. 71 (2000), H. 1-2 Y1 - 2000 SN - 0925-4005 SP - 13 EP - 18 ER - TY - JOUR A1 - Näther, Niko A1 - Auger, V. A1 - Poghossian, Arshak A1 - Koudelka-Hep, M. A1 - Schöning, Michael Josef T1 - A miniaturized flow-through cell in SU-8 technique for EIS sensors JF - Biomedizinische Technik. 49 (2004), H. 2 Y1 - 2004 SN - 0932-4666 SP - 994 EP - 995 ER - TY - JOUR A1 - Moreno i Codinachs, L. A1 - Birkenstock, C. A1 - Garma, T. A1 - Zierold, R. A1 - Bachmann, J. A1 - Nielsch, K. A1 - Schöning, Michael Josef A1 - Fontcuberta i Morral, A. T1 - A micron-sized nanoporous multifunction sensing device JF - physica status solidi (a) . 206 (2009), H. 3 Y1 - 2009 SN - 1862-6319 N1 - Special Issue: Engineering of Functional Interfaces (EnFI 08) SP - 435 EP - 441 PB - Wiley CY - Weinheim ER - TY - JOUR A1 - Schöning, Michael Josef A1 - Thust, M. A1 - Vetter, J. A1 - Kordos, P. (u.a.) T1 - A long-term stable penicillin-sensitive potentiometric biosensor with enzyme immobilized by heterobifunctional crosslinking Y1 - 1996 SN - 0378-4304 SP - 115 EP - 121 ER - TY - JOUR A1 - Simonis, A. A1 - Ruge, C. A1 - Müller-Veggian, Mattea A1 - Lüth, H. A1 - Schöning, Michael Josef T1 - A long-term stable macroporoustype EIS structure for electrochemical sensor applications JF - Sensors and Actuators B. 91 (2003), H. 1-3 Y1 - 2003 SN - 0925-4005 SP - 21 EP - 25 ER - TY - JOUR A1 - Wagner, Torsten A1 - Molina, R. A1 - Biselli, Manfred A1 - Canzoneri, Michele A1 - Schnitzler, Thomas A1 - Yoshinobu, Tatsuo A1 - Schöning, Michael Josef T1 - A light-addressable potentiometric sensor system for fast, simultaneous and spatial detection of the metabolic activity of biological cells JF - Transducers '07 Eurosensors XXI : digest of technical papers ; the14th International Conference on Solid-State Sensors, Actuators and Microsystems, June 10-14, 2007, Lyon, France / Gilles Delapierre (Ed.) Y1 - 2007 SN - 1-4244-0841-5 N1 - Eurosensors 21, 2007, Lyon ; International Conference on Solid-State Sensors, Actuators and Microsystems 14, 2007, Lyon SP - 1107 EP - 1110 PB - IEEE CY - Piscataway ER - TY - JOUR A1 - Dantism, Shahriar A1 - Röhlen, Desiree A1 - Wagner, Torsten A1 - Wagner, P. A1 - Schöning, Michael Josef T1 - A LAPS-based differential sensor for parallelized metabolism monitoring of various bacteria JF - Sensors N2 - Monitoring the cellular metabolism of bacteria in (bio)fermentation processes is crucial to control and steer them, and to prevent undesired disturbances linked to metabolically inactive microorganisms. In this context, cell-based biosensors can play an important role to improve the quality and increase the yield of such processes. This work describes the simultaneous analysis of the metabolic behavior of three different types of bacteria by means of a differential light-addressable potentiometric sensor (LAPS) set-up. The study includes Lactobacillus brevis, Corynebacterium glutamicum, and Escherichia coli, which are often applied in fermentation processes in bioreactors. Differential measurements were carried out to compensate undesirable influences such as sensor signal drift, and pH value variation during the measurements. Furthermore, calibration curves of the cellular metabolism were established as a function of the glucose concentration or cell number variation with all three model microorganisms. In this context, simultaneous (bio)sensing with the multi-organism LAPS-based set-up can open new possibilities for a cost-effective, rapid detection of the extracellular acidification of bacteria on a single sensor chip. It can be applied to evaluate the metabolic response of bacteria populations in a (bio)fermentation process, for instance, in the biogas fermentation process. Y1 - 2019 U6 - http://dx.doi.org/10.3390/s19214692 SN - 1424-8220 VL - 19 IS - 21 PB - MDPI CY - Basel ER - TY - JOUR A1 - Schöning, Michael Josef A1 - Tsarouchas, D. A1 - Schaub, A. A1 - Beckers, L. (u.a.) T1 - A highly long-term stable silicon-based pH sensor using pulsed laser deposition technique JF - Sensors and Actuators B. 35 (1996), H. 1-3 Y1 - 1996 SN - 0925-4005 SP - 228 EP - 233 ER - TY - JOUR A1 - Miyamoto, Ko-ichiro A1 - Hayashi, Kosuke A1 - Sakamoto, Azuma A1 - Werner, Frederik A1 - Wagner, Torsten A1 - Schöning, Michael Josef A1 - Yoshinobu, Tatsuo T1 - A high-Q resonance-mode measurement of EIS capacitive sensor by elimination of series resistance JF - Sensor and Actuators B: Chemical N2 - An EIS capacitive sensor is a semiconductor-based potentiometric sensor, which is sensitive to the ion concentration or pH value of the solution in contact with the sensing surface. To detect a small change in the ion concentration or pH, a small capacitance change must be detected. Recently, a resonance-mode measurement was proposed, in which an inductor was connected to the EIS capacitive sensor and the resonant frequency was correlated with the pH value. In this study, the Q factor of the resonant circuit was enhanced by canceling the internal resistance of the reference electrode and the internal resistance of the inductor coil with the help of a bypass capacitor and a negative impedance converter, respectively. 1% variation of the signal in the developed system corresponded to a pH change of 3.93 mpH, which was about 1/12 of the conventional method, suggesting a better performance in detection of a small pH change. KW - Negative impedance convertor KW - Resonance-mode measurement KW - Chemical sensor KW - EIS capacitive sensor Y1 - 2017 U6 - http://dx.doi.org/10.1016/j.snb.2017.03.002 SN - 0925-4005 VL - 248 SP - 1006 EP - 1010 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Wagner, Torsten A1 - Werner, Frederik A1 - Miyamoto, K. A1 - Schöning, Michael Josef A1 - Yoshinobu, T. T1 - A high-density multi-point LAPS set-up using a VCSEL array and FPGA control JF - Procedia Chemistry. 1 (2009), H. 1 Y1 - 2009 SN - 1876-6196 N1 - Proceedings of the Eurosensors XXIII conference ; Eurosensors 23 SP - 1483 EP - 1486 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Wagner, Torsten A1 - Werner, Frederik A1 - Miyamoto, Ko-Ichiro A1 - Schöning, Michael Josef A1 - Yoshinobu, Tatsuo T1 - A high-density multi-point LAPS set-up using a VCSEL array and FPGA control JF - Sensors and Actuators B: Chemical. 154 (2011), H. 2 Y1 - 2011 SN - 1873-3077 N1 - EUROSENSORS XXIII SP - 124 EP - 128 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Wagner, Torsten A1 - Schöning, Michael Josef A1 - Otto, R. A1 - Yoshinobu, T. T1 - A handheld 16 channel pen-type LAPS as a platform for (bio-)electrochemical sensing JF - Biomedizinische Technik. 49 (2004), H. 2 Y1 - 2004 SN - 0932-4666 SP - 996 EP - 997 ER - TY - JOUR A1 - Schöning, Michael Josef A1 - Kloock, Joachim P. A1 - Mourzina, Y. A1 - Schubert, J. T1 - A first step towards a microfabricated thin-film sensor array on the basis of chalcogenide glass materials JF - Sensors. 2 (2002), H. 9 Y1 - 2002 SN - 1424-8220 SP - 356 EP - 365 ER - TY - JOUR A1 - Schöning, Michael Josef A1 - Krause, R. A1 - Block, K. A1 - Musahmeh, M. A1 - Mulchandani, A. A1 - Wang, J. T1 - A dual amperometric/potentiometric FIA-based biosensor for the distinctive detection of organophosphorus pesticides JF - Sensors and Actuators B. 95 (2003), H. 1-3 Y1 - 2003 SN - 0925-4005 SP - 291 EP - 296 ER - TY - JOUR A1 - Schöning, Michael Josef A1 - Krause, R. A1 - Block, K. A1 - Musameh, M. A1 - Mulchandani, A. A1 - Wang, J. T1 - A dual amperometric / potentiometric FIA-based biosensor for the distinctive detection of organophosphorus pesticides JF - Book of abstracts / ed. by J. Saneistr. Y1 - 2002 SN - 80-01-02576-4 N1 - Eurosensors ; (16, 2002, Praha) SP - 76 EP - 79 PB - Czech Technical University, Faculty of Electrical Engineering, Department of Measurement CY - Prague ER - TY - JOUR A1 - Bohrn, Ulrich A1 - Mucha, Andreas A1 - Werner, Frederik A1 - Trattner, Barbara A1 - Bäcker, Matthias A1 - Krumbe, Christoph A1 - Schienle, Meinrad A1 - Stütz, Evamaria A1 - Schmitt-Landsiedel, Doris A1 - Fleischer, Maximilian A1 - Wagner, Patrick A1 - Schöning, Michael Josef T1 - A critical comparison of cell-based sensor systems for the detection of Cr (VI) in aquatic environment JF - Sensors and actuators. B: Chemical Y1 - 2013 SN - 1873-3077 (E-Journal); 0925-4005 (Print) VL - Vol. 182 SP - 58 EP - 65 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Schöning, Michael Josef A1 - Arzdorf, M. A1 - Mulchandani, P. A1 - Chen, W. A1 - Mulchandani, A. T1 - A capacitive field-effect sensor for the direct determination of organophosphorus pesticides JF - Sensors and Actuators B. 91 (2003), H. 1-3 Y1 - 2003 SN - 0925-4005 SP - 92 EP - 97 ER - TY - JOUR A1 - Schöning, Michael Josef A1 - Crott, M. A1 - Ronkel, F. A1 - Thust, M. A1 - Schultze, J. W. A1 - Kordos, P. A1 - Lüth, H. T1 - A capacitive chemical sensor based on porous silicon JF - Proceedings of the 26th European Solid State Device Research Conference : Congress Centre, Bologna, Italy, 9 - 11 September 1996 / ESSDERC '96. Ed. by G. Baccarani Y1 - 1996 SN - 2-86332-196-X N1 - ESSDERC ; (26, 1996, Bologna) ; European Solid State Device Research Conference (ESSDERC) SP - 907 EP - 910 PB - Ed. Frontières CY - Gif-sur-Yvette ER - TY - JOUR A1 - Schöning, Michael Josef A1 - Schütz, S. A1 - Schroth, P. A1 - Weißbecker, B. A1 - Steffen, A. A1 - Kordos, P. A1 - Hummel, Hans E. T1 - A BioFET on the basis of intact insect antennae JF - Sensors and Actuators B. 47 (1998), H. 1-3 Y1 - 1998 SN - 0925-4005 SP - 235 EP - 238 ER - TY - JOUR A1 - Schöning, Michael Josef A1 - Schütz, S. A1 - Riemer, A. A1 - Weißbecker, B. A1 - Steffen, A. A1 - Kordos, P. A1 - Lüth, H. A1 - Hummel, Hans E. T1 - A BioFET on the basis of intact insect antennae JF - Proceedings of the 11th European Conference on Solid-State Transducers / Eurosensors XI, September 21 - 24, 1997, Warsaw, Poland. [Organised by] Warsaw University of Technology. Vol 2. Y1 - 1997 SN - 83-908335-0-6 N1 - Eurosensors ; (11, 1997, Warszawa) ; Eurosensors - European Conference on Solid-State Transducers ; (11 : ; 1997.09.21-24 : ; Warsaw) SP - 1461 EP - 1464 PB - Sensor Lab Sp. CY - Warsaw ER - TY - JOUR A1 - Schöning, Michael Josef A1 - Simonis, Anette A1 - Ruge, Christian A1 - Ecken, Holger A1 - Müller-Veggian, Mattea A1 - Lüth, Hans T1 - A (bio-)chemical Field-effect Sensor with Macroporous Si as Substrate Material and a SiO₂ / LPCVD-Si₃N₄ Double Layer as pH Transducer JF - Sensors. 2 (2002), H. 1 N2 - Macroporous silicon has been etched from n-type Si, using a vertical etching cell where no rear side contact on the silicon wafer is necessary. The resulting macropores have been characterised by means of Scanning Electron Microscopy (SEM). After etching, SiO₂ was thermally grown on the top of the porous silicon as an insulating layer and Si₃N₄ was deposited by means of Low Pressure Chemical Vapour Deposition (LPCVD) as transducer material to fabricate a capacitive pH sensor. In order to prepare porous biosensors, the enzyme penicillinase has been additionally immobilised inside the porous structure. Electrochemical measurements of the pH sensor and the biosensor with an Electrolyte/Insulator/Semiconductor (EIS) structure have been performed in the Capacitance/Voltage (C/V) and Constant capacitance (ConCap) mode. Y1 - 2002 SN - 1424-8220 U6 - http://dx.doi.org/10.3390/s20100011 SP - 11 EP - 22 ER - TY - JOUR A1 - Muschallik, Lukas A1 - Molinnus, Denise A1 - Bongaerts, Johannes A1 - Pohl, Martina A1 - Wagner, Torsten A1 - Schöning, Michael Josef A1 - Siegert, Petra A1 - Selmer, Thorsten T1 - (R,R)-Butane-2,3-diol Dehydrogenase from Bacillus clausii DSM 8716T: Cloning and Expression of the bdhA-Gene, and Initial Characterization of Enzyme JF - Journal of Biotechnology N2 - The gene encoding a putative (R,R)-butane-2,3-diol dehydrogenase (bdhA) from Bacillus clausii DSM 8716T was isolated, sequenced and expressed in Escherichia coli. The amino acid sequence of the encoded protein is only distantly related to previously studied enzymes (identity 33–43%) and exhibited some uncharted peculiarities. An N-terminally StrepII-tagged enzyme variant was purified and initially characterized. The isolated enzyme catalyzed the (R)-specific oxidation of (R,R)- and meso-butane-2,3-diol to (R)- and (S)-acetoin with specific activities of 12 U/mg and 23 U/mg, respectively. Likewise, racemic acetoin was reduced with a specific activity of up to 115 U/mg yielding a mixture of (R,R)- and meso-butane-2,3-diol, while the enzyme reduced butane-2,3-dione (Vmax 74 U/mg) solely to (R,R)-butane-2,3-diol via (R)-acetoin. For these reactions only activity with the co-substrates NADH/NAD+ was observed. The enzyme accepted a selection of vicinal diketones, α-hydroxy ketones and vicinal diols as alternative substrates. Although the physiological function of the enzyme in B. clausii remains elusive, the data presented herein clearly demonstrates that the encoded enzyme is a genuine (R,R)-butane-2,3-diol dehydrogenase with potential for applications in biocatalysis and sensor development. Y1 - 2017 U6 - http://dx.doi.org/10.1016/j.jbiotec.2017.07.020 SN - 0168-1656 VL - 258 SP - 41 EP - 50 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Poghossian, Arshak A1 - Lüth, H. A1 - Schultze, J. W. A1 - Schöning, Michael Josef T1 - (Bio-)chemical and physical microsensor array using an identical transducer principle JF - Scaling down in electrochemistry : electrochemical micro- and nanosystem technology ; proceedings of the 3rd International Symposium on Electrochemical Microsystem Technologies, Garmisch-Patenkirchen, Germany, 11 - 15 September 2000 / ed. by J. W. Schultz Y1 - 2001 SN - 0-08-044014-2 SP - 243 EP - 249 PB - Elsevier [u.a.] CY - Amsterdam [u.a.] ER - TY - JOUR A1 - Buniatyan, V. V. A1 - Matirosyan, N. W. A1 - Abouzar, Maryam H. A1 - Schöning, Michael Josef A1 - Poghossian, Arshak A1 - Schubert, J. A1 - Khachatryan, V. R. A1 - Soukiassyan, G. R. T1 - (Ba,Sr)TiO3 based electrolyte-insulator-semiconductor pHsensors: comparitive study JF - Semiconductor micro- and nanoelectronics : Proceedings of the Seventh International Conference , Tsakhcadzor, Armenia July 3-5 2009 Y1 - 2009 SP - 71 EP - 74 ER - TY - JOUR A1 - Schöning, Michael Josef T1 - "Voltohmmetry" – a new transducer principle for electrochemical sensors JF - Ultrathin electrochemical chemo- and biosensors : technology and performance / Vladimir M. Mirsky Y1 - 2004 SN - 3-540-21285-X SP - 117 EP - 140 PB - Springer CY - Berlin [u.a.] ER - TY - JOUR A1 - Schöning, Michael Josef T1 - "Playing around“ with field-effect sensors on the basis of EIS structures, LAPS and ISFETs JF - Sensors. 5 (2005), H. 3 Y1 - 2005 SN - 1424-8220 SP - 126 EP - 138 ER - TY - JOUR A1 - Poghossian, Arshak A1 - Schöning, Michael Josef T1 - "High-order” hybrid FET module for (bio)chemical and physical sensing JF - Integrated analytical systems / ed. by Salvador Alegret Y1 - 2003 SN - 0-444-51037-0 SP - 587 EP - 623 PB - Elsevier CY - Amsterdam [u.a.] ER -