TY  - JOUR
A1  - Savitskaya, Irina
A1  - Zhantlessova, Sirina
A1  - Kistaubayeva, Aida
A1  - Ignatova, Ludmila
A1  - Shokatayeva, Dina
A1  - Sinyavsky, Yuriy
A1  - Kushugulova, Almagul
A1  - Digel, Ilya
T1  - Prebiotic cellulose–pullulan matrix as a “vehicle” for probiotic biofilm delivery to the host large intestine
JF  - Polymers
N2  - This study describes the development of a new combined polysaccharide-matrix-based technology for the immobilization of Lactobacillus rhamnosus GG (LGG) bacteria in biofilm form. The new composition allows for delivering the bacteria to the digestive tract in a manner that improves their robustness compared with planktonic cells and released biofilm cells. Granules consisting of a polysaccharide matrix with probiotic biofilms (PMPB) with high cell density (>9 log CFU/g) were obtained by immobilization in the optimized nutrient medium. Successful probiotic loading was confirmed by fluorescence microscopy and scanning electron microscopy. The developed prebiotic polysaccharide matrix significantly enhanced LGG viability under acidic (pH 2.0) and bile salt (0.3%) stress conditions. Enzymatic extract of feces, mimicking colon fluid in terms of cellulase activity, was used to evaluate the intestinal release of probiotics. PMPB granules showed the ability to gradually release a large number of viable LGG cells in the model colon fluid. In vivo, the oral administration of PMPB granules in rats resulted in the successful release of probiotics in the colon environment. The biofilm-forming incubation method of immobilization on a complex polysaccharide matrix tested in this study has shown high efficacy and promising potential for the development of innovative biotechnologies.
KW  - immobilization
KW  - prebiotic
KW  - bacterial cellulose
KW  - biofilms
KW  - Lactobacillus rhamnosus GG
Y1  - 2023
U6  - http://dx.doi.org/10.3390/polym16010030
N1  - This article belongs to the Section "Polymer Composites and Nanocomposites"
IS  - 16(1)
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Zhantlessova, Sirina
A1  - Savitskaya, Irina
A1  - Kistaubayeva, Aida
A1  - Ignatova, Ludmila
A1  - Talipova, Aizhan
A1  - Pogrebnjak, Alexander
A1  - Digel, Ilya
T1  - Advanced “Green” prebiotic composite of bacterial cellulose/pullulan based on synthetic biology-powered microbial coculture strategy
JF  - Polymers
N2  - Bacterial cellulose (BC) is a biopolymer produced by different microorganisms, but in biotechnological practice, Komagataeibacter xylinus is used. The micro- and nanofibrillar structure of BC, which forms many different-sized pores, creates prerequisites for the introduction of other polymers into it, including those synthesized by other microorganisms. The study aims to develop a cocultivation system of BC and prebiotic producers to obtain BC-based composite material with prebiotic activity. In this study, pullulan (PUL) was found to stimulate the growth of the probiotic strain Lactobacillus rhamnosus GG better than the other microbial polysaccharides gellan and xanthan. BC/PUL biocomposite with prebiotic properties was obtained by cocultivation of Komagataeibacter xylinus and Aureobasidium pullulans, BC and PUL producers respectively, on molasses medium. The inclusion of PUL in BC is proved gravimetrically by scanning electron microscopy and by Fourier transformed infrared spectroscopy. Cocultivation demonstrated a composite effect on the aggregation and binding of BC fibers, which led to a significant improvement in mechanical properties. The developed approach for “grafting” of prebiotic activity on BC allows preparation of environmentally friendly composites of better quality.
KW  - coculture
KW  - pullulan
KW  - exopolysaccharides
KW  - prebiotic
KW  - bacterial cellulose
Y1  - 2022
U6  - http://dx.doi.org/10.3390/polym14153224
SN  - 2073-4360
N1  - This article belongs to the Special Issue "Cellulose Based Composites"
VL  - 14
IS  - 15
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Turlybekuly, Amanzhol
A1  - Pogrebnjak, Alexander
A1  - Sukhodub, L. F.
A1  - Sukhodub, Liudmyla B.
A1  - Kistaubayeva, A. S.
A1  - Savitskaya, Irina
A1  - Shokatayeva, D. H.
A1  - Bondar, Oleksandr V.
A1  - Shaimardanov, Z. K.
A1  - Plotnikov, Sergey V.
A1  - Shaimardanova, B. H.
A1  - Digel, Ilya
T1  - Synthesis, characterization, in vitro biocompatibility and antibacterial properties study of nanocomposite materials based on hydroxyapatite-biphasic ZnO micro- and nanoparticles embedded in Alginate matrix
JF  - Materials Science and Engineering C
Y1  - 2019
U6  - http://dx.doi.org/10.1016/j.msec.2019.109965
VL  - 104
IS  - Article number 109965
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Sadykov, Rustam
A1  - Digel, Ilya
A1  - Temiz Artmann, Aysegül
A1  - Porst, Dariusz
A1  - Linder, Peter
A1  - Kayser, Peter
A1  - Artmann, Gerhard
A1  - Savitskaya, Irina
A1  - Zhubanova, Azhar
T1  - Oral lead exposure induces dysbacteriosis in rats
JF  - Journal of Occupational Health. 51 (2009) (2009), H. 1
Y1  - 2009
SN  - 1348-9585
SP  - 64
EP  - 73
ER  -