TY - JOUR A1 - Pogorelova, Natalia A1 - Rogachev, Evgeniy A1 - Digel, Ilya A1 - Chernigova, Svetlana A1 - Nardin, Dmitry T1 - Bacterial Cellulose Nanocomposites: Morphology and Mechanical Properties JF - Materials N2 - Bacterial cellulose (BC) is a promising material for biomedical applications due to its unique properties such as high mechanical strength and biocompatibility. This article describes the microbiological synthesis, modification, and characterization of the obtained BC-nanocomposites originating from symbiotic consortium Medusomyces gisevii. Two BC-modifications have been obtained: BC-Ag and BC-calcium phosphate (BC-Ca3(PO4)2). Structure and physicochemical properties of the BC and its modifications were investigated by scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), atomic force microscopy (AFM), and infrared Fourier spectroscopy as well as by measurements of mechanical and water holding/absorbing capacities. Topographic analysis of the surface revealed multicomponent thick fibrils (150–160 nm in diameter and about 15 µm in length) constituted by 50–60 nm nanofibrils weaved into a left-hand helix. Distinctive features of Ca-phosphate-modified BC samples were (a) the presence of 500–700 nm entanglements and (b) inclusions of Ca3(PO4)2 crystals. The samples impregnated with Ag nanoparticles exhibited numerous roundish inclusions, about 110 nm in diameter. The boundaries between the organic and inorganic phases were very distinct in both cases. The Ag-modified samples also showed a prominent waving pattern in the packing of nanofibrils. The obtained BC gel films possessed water-holding capacity of about 62.35 g/g. However, the dried (to a constant mass) BC-films later exhibited a low water absorption capacity (3.82 g/g). It was found that decellularized BC samples had 2.4 times larger Young’s modulus and 2.2 times greater tensile strength as compared to dehydrated native BC films. We presume that this was caused by molecular compaction of the BC structure. Y1 - 2020 SN - 1996-1944 U6 - http://dx.doi.org/10.3390/ma13122849 VL - 13 IS - 12 SP - 1 EP - 16 PB - MDPI CY - Basel ER - TY - CHAP A1 - Zhubanova, Azhar A. A1 - Mansurov, Zulkhair A. A1 - Digel, Ilya T1 - Use of Advanced Nanomaterials for Bioremediation of Contaminated Ecosystems T2 - Carbon Nanomaterials in Biomedicine and the Environment N2 - This chapter shows that nanomaterials obtained by high-temperature carbonization of inexpensive plant raw material such as rice husk, grape seeds, and walnut shells can serve as a basis for the production of highly efficient microbial drugs, biodestructors, biosorbents, and biocatalysts, which are promising for the remediation of the ecosystem contaminated with heavy and radioactive metals, oil and oil products. A strong interest in engineering zymology is dictated by the necessity to address the issues of monitoring enzymatic processes, treatment, and diagnosis of a number of common human diseases, environmental pollution, quality control of pharmaceuticals and food. Nanomaterials obtained by high-temperature carbonization of cheap plant raw material such as-rice husks, grape seeds and walnut shells, can serve as a basis for creating of highly effective microbial preparations-biodestructors, biosorbents and biocatalysts, which are promising for the use of contaminated ecosystems, and for restoration of human intestine microecology. Y1 - 2020 SN - 978-981-4800-27-3 U6 - http://dx.doi.org/10.1201/9780429428647-18 SP - 353 EP - 378 PB - Jenny Stanford Publishing CY - Singapore ER - TY - CHAP A1 - Savitskaya, Irina S. A1 - Kistaubayeva, Aida S. A1 - Akimbekov, Nuraly A1 - Digel, Ilya A1 - Shokatayeva, Dina A1 - Zhubanova, Azhar Achmet T1 - Prospective Use of Probiotics Immobilized on Sorbents with Nanostructured Surfaces T2 - Carbon Nanomaterials in Biomedicine and the Environment N2 - Activated carbons are known as excellent adsorbents. Their applications include the adsorptive removal of color, odor, taste, undesirable organic and inorganic pollutants from drinking and waste water; air purification in inhabited spaces; purification of many chemicals, pharmaceutical products and many others. This chapter elucidates the role of normal microflora in the maintenance of human health and presents materials on possible clinical displays of microecological infringements and ways of their correction. It presents new developments concerning new probiotics with immobilized Lactobacillus and Bacillus. The chapter considers the mechanisms of the intestine disbacteriosis correction by sorbed probiotics. It demonstrates the advantages and creation prospects of immobilized probiotics developed on the basis of carbonized rice husk. There are great prospects for the development of medical biotechnology due to use of carbon sorbents with a nanostructured surface. Microbial communities form a biocenosis of the biotope and together with the host organism create permanent or temporary ecosystems. Y1 - 2020 SN - 978-981-4800-27-3 U6 - http://dx.doi.org/10.1201/9780429428647-12 SP - 229 EP - 267 PB - Jenny Stanford Publishing CY - Singapore ER - TY - CHAP A1 - Akimbekov, Nuraly A1 - Zhanadilovna, Abdieva G. A1 - Ualieva, Perizat S. A1 - Abaihanovna, Zhusipova D. A1 - Digel, Ilya A1 - Savitskaya, Irina S. A1 - Zhubanova, Azhar Achmet T1 - Functionalization of Carbon Based Wound Dressings with Antimicrobial Phytoextracts for Bioactive Treatment of Septic Wounds T2 - Carbon Nanomaterials in Biomedicine and the Environment N2 - The treatment of septic wounds with curative dressings based on biocomposites containing sage and marigold phytoextracts was effective in in vitro and in vivo experiments. These dressings caused the purification of the wound surface from purulent-necrotic masses three days earlier than in the other experimental groups. The consequence of an increase in incidents of severe course of the wound and the observed tendency to increase the number of adverse effects is the development of long-term recurrent wound processes. To treat purulent wounds, the following tactics were used: The purulent wounds of animals were covered with the examined wound dressing, and then the next day samples were taken, the procedure was performed once in 2 days. To obtain the active nanostructured sorbents such as carbonized rice husks, they are functionalized with biologically active components possessing antimicrobial, anti-inflammatory, antitoxic, immunomodulating, antiallergic and other types of properties. Y1 - 2020 SN - 978-981-4800-27-3 U6 - http://dx.doi.org/10.1201/9780429428647-11 SP - 211 EP - 228 PB - Jenny Stanford Publishing CY - Singapore ER - TY - CHAP A1 - Mansurov, Zulkhair A. A1 - Jandosov, Jakpar A1 - Chenchik, D. A1 - Azat, Seitkhan A1 - Savitskaya, Irina S. A1 - Kistaubaeva, Aida A1 - Akimbekov, Nuraly A1 - Digel, Ilya A1 - Zhubanova, Azhar Achmet T1 - Biocomposite Materials Based on Carbonized Rice Husk in Biomedicine and Environmental Applications T2 - Carbon Nanomaterials in Biomedicine and the Environment N2 - This chapter describes the prospects for biomedical and environmental engineering applications of heterogeneous materials based on nanostructured carbonized rice husk. Efforts in engineering enzymology are focused on the following directions: development and optimization of immobilization methods leading to novel biotechnological and biomedical applications; construction of biocomposite materials based on individual enzymes, multi-enzyme complexes and whole cells, targeted on realization of specific industrial processes. Molecular biological and biochemical studies on cell adhesion focus predominantly on identification, isolation and structural analysis of attachment-responsible biological molecules and their genetic determinants. The chapter provides a short overview of applications of the biocomposite materials based of nanostructured carbonized adsorbents. It emphasizes that further studies and better understanding of the interactions between CNS and microbial cells are necessary. The future use of living cells as biocatalysts, especially in the environmental field, needs more systematic investigations of the microbial adsorption phenomenon. Y1 - 2020 SN - 978-981-4800-27-3 U6 - http://dx.doi.org/10.1201/9780429428647-2 SP - 3 EP - 32 PB - Jenny Stanford Publishing Pte. Ltd. CY - Singapore ER - TY - JOUR A1 - Jablonski, Melanie A1 - Poghossian, Arshak A1 - Severin, Robin A1 - Keusgen, Michael A1 - Wege, Christian A1 - Schöning, Michael Josef T1 - Capacitive Field-Effect Biosensor Studying Adsorption of Tobacco Mosaic Virus Particles JF - Micromachines N2 - Plant virus-like particles, and in particular, tobacco mosaic virus (TMV) particles, are increasingly being used in nano- and biotechnology as well as for biochemical sensing purposes as nanoscaffolds for the high-density immobilization of receptor molecules. The sensitive parameters of TMV-assisted biosensors depend, among others, on the density of adsorbed TMV particles on the sensor surface, which is affected by both the adsorption conditions and surface properties of the sensor. In this work, Ta₂O₅-gate field-effect capacitive sensors have been applied for the label-free electrical detection of TMV adsorption. The impact of the TMV concentration on both the sensor signal and the density of TMV particles adsorbed onto the Ta₂O₅-gate surface has been studied systematically by means of field-effect and scanning electron microscopy methods. In addition, the surface density of TMV particles loaded under different incubation times has been investigated. Finally, the field-effect sensor also demonstrates the label-free detection of penicillinase immobilization as model bioreceptor on TMV particles. KW - capacitive field-effect sensor KW - plant virus detection KW - tobacco mosaic virus (TMV) KW - TMV adsorption KW - Ta₂O₅ gate Y1 - 2021 U6 - http://dx.doi.org/10.3390/mi12010057 VL - 12 IS - 1 PB - MDPI CY - Basel ER - TY - JOUR A1 - Poghossian, Arshak A1 - Jablonski, Melanie A1 - Molinnus, Denise A1 - Wege, Christina A1 - Schöning, Michael Josef T1 - Field-Effect Sensors for Virus Detection: From Ebola to SARS-CoV-2 and Plant Viral Enhancers JF - Frontiers in Plant Science N2 - Coronavirus disease 2019 (COVID-19) is a novel human infectious disease provoked by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Currently, no specific vaccines or drugs against COVID-19 are available. Therefore, early diagnosis and treatment are essential in order to slow the virus spread and to contain the disease outbreak. Hence, new diagnostic tests and devices for virus detection in clinical samples that are faster, more accurate and reliable, easier and cost-efficient than existing ones are needed. Due to the small sizes, fast response time, label-free operation without the need for expensive and time-consuming labeling steps, the possibility of real-time and multiplexed measurements, robustness and portability (point-of-care and on-site testing), biosensors based on semiconductor field-effect devices (FEDs) are one of the most attractive platforms for an electrical detection of charged biomolecules and bioparticles by their intrinsic charge. In this review, recent advances and key developments in the field of label-free detection of viruses (including plant viruses) with various types of FEDs are presented. In recent years, however, certain plant viruses have also attracted additional interest for biosensor layouts: Their repetitive protein subunits arranged at nanometric spacing can be employed for coupling functional molecules. If used as adapters on sensor chip surfaces, they allow an efficient immobilization of analyte-specific recognition and detector elements such as antibodies and enzymes at highest surface densities. The display on plant viral bionanoparticles may also lead to long-time stabilization of sensor molecules upon repeated uses and has the potential to increase sensor performance substantially, compared to conventional layouts. This has been demonstrated in different proof-of-concept biosensor devices. Therefore, richly available plant viral particles, non-pathogenic for animals or humans, might gain novel importance if applied in receptor layers of FEDs. These perspectives are explained and discussed with regard to future detection strategies for COVID-19 and related viral diseases. Y1 - 2020 U6 - http://dx.doi.org/10.3389/fpls.2020.598103 VL - 11 IS - Article 598103 SP - 1 EP - 14 PB - Frontiers CY - Lausanne ER - TY - JOUR A1 - Gossmann, Matthias A1 - Thomas, Ulrich A1 - Horváth, András A1 - Dragicevic, Elena A1 - Stoelzle-Feix, Sonja A1 - Jung, Alexander A1 - Raman, Aravind Hariharan A1 - Staat, Manfred A1 - Linder, Peter T1 - A higher-throughput approach to investigate cardiac contractility in vitro under physiological mechanical conditions JF - Journal of Pharmacological and Toxicological Methods Y1 - 2020 U6 - http://dx.doi.org/10.1016/j.vascn.2020.106843 VL - 105 IS - Article 106843 PB - Elsevier CY - New York, NY ER - TY - CHAP A1 - Bozakov, Zdravko A1 - Sander, Volker T1 - OpenFlow: A Perspective for Building Versatile Networks T2 - Network-Embedded Management and Applications Y1 - 2013 SN - 978-1-4419-6769-5 U6 - http://dx.doi.org/10.1007/978-1-4419-6769-5_11 SP - 217 EP - 245 PB - Springer CY - New York, NY ER - TY - JOUR A1 - Conzen, Catharina A1 - Albanna, Walid A1 - Weiss, Miriam A1 - Kürten, David A1 - Vilser, Walthard A1 - Kotliar, Konstantin A1 - Zäske, Charlotte A1 - Clusmann, Hans A1 - Schubert, Gerrit Alexander T1 - Vasoconstriction and Impairment of Neurovascular Coupling after Subarachnoid Hemorrhage: a Descriptive Analysis of Retinal Changes JF - Translational Stroke Research N2 - Impaired cerebral autoregulation and neurovascular coupling (NVC) contribute to delayed cerebral ischemia after subarachnoid hemorrhage (SAH). Retinal vessel analysis (RVA) allows non-invasive assessment of vessel dimension and NVC hereby demonstrating a predictive value in the context of various neurovascular diseases. Using RVA as a translational approach, we aimed to assess the retinal vessels in patients with SAH. RVA was performed prospectively in 24 patients with acute SAH (group A: day 5–14), in 11 patients 3 months after ictus (group B: day 90 ± 35), and in 35 age-matched healthy controls (group C). Data was acquired using a Retinal Vessel Analyzer (Imedos Systems UG, Jena) for examination of retinal vessel dimension and NVC using flicker-light excitation. Diameter of retinal vessels—central retinal arteriolar and venular equivalent—was significantly reduced in the acute phase (p < 0.001) with gradual improvement in group B (p < 0.05). Arterial NVC of group A was significantly impaired with diminished dilatation (p < 0.001) and reduced area under the curve (p < 0.01) when compared to group C. Group B showed persistent prolonged latency of arterial dilation (p < 0.05). Venous NVC was significantly delayed after SAH compared to group C (A p < 0.001; B p < 0.05). To our knowledge, this is the first clinical study to document retinal vasoconstriction and impairment of NVC in patients with SAH. Using non-invasive RVA as a translational approach, characteristic patterns of compromise were detected for the arterial and venous compartment of the neurovascular unit in a time-dependent fashion. Recruitment will continue to facilitate a correlation analysis with clinical course and outcome. Y1 - 2018 U6 - http://dx.doi.org/10.1007/s12975-017-0585-8 SN - 1868-601X IS - 9 SP - 284 EP - 293 PB - Springer Nature CY - Cham ER - TY - JOUR A1 - Stulpe, Werner T1 - Pairwise coexistence of effects versus coexistence JF - Journal of Physics: Conference Series Y1 - 2020 U6 - http://dx.doi.org/10.1088/1742-6596/1638/1/012004 SN - 1742-6596 VL - 1638 IS - 012004 SP - 1 EP - 21 PB - IOP CY - Bristol ER - TY - CHAP A1 - Iomdina, Elena N. A1 - Kiseleva, Anna A. A1 - Kotliar, Konstantin A1 - Luzhnov, Petr V. T1 - Quantification of Choroidal Blood Flow Using the OCT-A System Based on Voxel Scan Processing T2 - 2020 International Conference on Biomedical Innovations and Applications (BIA) Y1 - 2020 SN - 978-1-7281-7073-2 U6 - http://dx.doi.org/10.1109/BIA50171.2020.9244511 SP - 41 EP - 44 ER - TY - BOOK A1 - Yoshinobu, Tatsuo A1 - Schöning, Michael Josef ED - Yoshinobu, Tatsuo ED - Schöning, Michael Josef T1 - Light-addressing and chemical imaging technologies for electrochemical sensing Y1 - 2020 SN - 978-3-03943-029-1 U6 - http://dx.doi.org/10.3390/books978-3-03943-029-1 N1 - This book is a printed edition of the Special Issue Light-Addressing and Chemical Imaging Technologies for Electrochemical Sensing that was published in Sensors PB - MDPI CY - Basel ER - TY - CHAP A1 - Sildatke, Michael A1 - Karwanni, Hendrik A1 - Kraft, Bodo A1 - Schmidts, Oliver A1 - Zündorf, Albert T1 - Automated Software Quality Monitoring in Research Collaboration Projects T2 - ICSEW'20: Proceedings of the IEEE/ACM 42nd International Conference on Software Engineering Workshops Y1 - 2020 U6 - http://dx.doi.org/10.1145/3387940.3391478 SP - 603 EP - 610 ER - TY - JOUR A1 - Malan, Leone A1 - Hamer, Mark A1 - Känel, Roland von A1 - Kotliar, Konstantin A1 - Wyk, Roelof D. van A1 - Lambert, Gavin W. A1 - Vilser, Walthard A1 - Ziemssen, Tjalf A1 - Schlaich, Markus P. A1 - Smith, Wayne A1 - Magnusson, Martin A1 - Wentzel, Annemarie A1 - Myburgh, Carlien E. A1 - Steyn, Hendrik S. A1 - Malan, Nico T. T1 - Delayed retinal vein recovery responses indicate both non-adaptation to stress as well as increased risk for stroke: the SABPA study JF - Cardiovascular Journal of Africa Y1 - 2020 U6 - http://dx.doi.org/10.5830/CVJA-2020-031 SN - 1680-0745 VL - 26 IS - 31 SP - 1 EP - 12 PB - Clinics Cardive Publishing CY - Durbanville ER - TY - JOUR A1 - Quittmann, Oliver J. A1 - Meskemper, Joshua A1 - Albracht, Kirsten A1 - Abel, Thomas A1 - Foitschik, Tina A1 - Strüder, Heiko K. T1 - Normalising surface EMG of ten upper-extremity muscles in handcycling: Manual resistance vs. sport-specific MVICs JF - Journal of Electromyography and Kinesiology N2 - Muscular activity in terms of surface electromyography (sEMG) is usually normalised to maximal voluntary isometric contractions (MVICs). This study aims to compare two different MVIC-modes in handcycling and examine the effect of moving average window-size. Twelve able-bodied male competitive triathletes performed ten MVICs against manual resistance and four sport-specific trials against fixed cranks. sEMG of ten muscles [M. trapezius (TD); M. pectoralis major (PM); M. deltoideus, Pars clavicularis (DA); M. deltoideus, Pars spinalis (DP); M. biceps brachii (BB); M. triceps brachii (TB); forearm flexors (FC); forearm extensors (EC); M. latissimus dorsi (LD) and M. rectus abdominis (RA)] was recorded and filtered using moving average window-sizes of 150, 200, 250 and 300 ms. Sport-specific MVICs were higher compared to manual resistance for TB, DA, DP and LD, whereas FC, TD, BB and RA demonstrated lower values. PM and EC demonstrated no significant difference between MVIC-modes. Moving average window-size had no effect on MVIC outcomes. MVIC-mode should be taken into account when normalised sEMG data are illustrated in handcycling. Sport-specific MVICs seem to be suitable for some muscles (TB, DA, DP and LD), but should be augmented by MVICs against manual/mechanical resistance for FC, TD, BB and RA. Y1 - 2020 U6 - http://dx.doi.org/10.1016/j.jelekin.2020.102402 SN - 1050-6411 VL - 51 IS - Article 102402 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Kopp, Alexander A1 - Schunck, Laura A1 - Gosau, Martin A1 - Smeets, Ralf A1 - Burg, Simon A1 - Fuest, Sandra A1 - Kröger, Nadja A1 - Zinser, Max A1 - Krohn, Sebastian A1 - Behbahani, Mehdi A1 - Köpf, Marius A1 - Lauts, Lisa A1 - Rutkowski, Rico T1 - Influence of the casting concentration on the mechanical and optical properties of Fa/CaCl2-derived silk fibroin membranes JF - International Journal of Molecular Sciences N2 - In this study, we describe the manufacturing and characterization of silk fibroin membranes derived from the silkworm Bombyx mori. To date, the dissolution process used in this study has only been researched to a limited extent, although it entails various potential advantages, such as reduced expenses and the absence of toxic chemicals in comparison to other conventional techniques. Therefore, the aim of this study was to determine the influence of different fibroin concentrations on the process output and resulting membrane properties. Casted membranes were thus characterized with regard to their mechanical, structural and optical assets via tensile testing, SEM, light microscopy and spectrophotometry. Cytotoxicity was evaluated using BrdU, XTT, and LDH assays, followed by live–dead staining. The formic acid (FA) dissolution method was proven to be suitable for the manufacturing of transparent and mechanically stable membranes. The fibroin concentration affects both thickness and transparency of the membranes. The membranes did not exhibit any signs of cytotoxicity. When compared to other current scientific and technical benchmarks, the manufactured membranes displayed promising potential for various biomedical applications. Further research is nevertheless necessary to improve reproducible manufacturing, including a more uniform thickness, less impurity and physiological pH within the membranes. Y1 - 2020 U6 - http://dx.doi.org/10.3390/ijms21186704 SN - 1422-0067 N1 - Special issue: Optimization of Biomaterials for Reconstructive and Regenerative Medicine VL - 21 IS - 18 art. no. 6704 PB - MDPI CY - Basel ER - TY - JOUR A1 - Poghossian, Arshak A1 - Schöning, Michael Josef T1 - Capacitive field-effect eis chemical sensors and biosensors: A status report JF - Sensors N2 - Electrolyte-insulator-semiconductor (EIS) field-effect sensors belong to a new generation of electronic chips for biochemical sensing, enabling a direct electronic readout. The review gives an overview on recent advances and current trends in the research and development of chemical sensors and biosensors based on the capacitive field-effect EIS structure—the simplest field-effect device, which represents a biochemically sensitive capacitor. Fundamental concepts, physicochemical phenomena underlying the transduction mechanism and application of capacitive EIS sensors for the detection of pH, ion concentrations, and enzymatic reactions, as well as the label-free detection of charged molecules (nucleic acids, proteins, and polyelectrolytes) and nanoparticles, are presented and discussed. Y1 - 2020 U6 - http://dx.doi.org/10.3390/s20195639 SN - 1424-8220 VL - 20 IS - 19 PB - MDPI CY - Basel ER - TY - JOUR A1 - Muschallik, Lukas A1 - Kipp, Carina Ronja A1 - Recker, Inga A1 - Bongaerts, Johannes A1 - Pohl, Martina A1 - Gelissen, Melanie A1 - Schöning, Michael Josef A1 - Selmer, Thorsten A1 - Siegert, Petra T1 - Synthesis of α-hydroxy ketones and vicinal diols with the Bacillus licheniformis DSM 13T butane-2, 3-diol dehydrogenase JF - Journal of Biotechnology N2 - The enantioselective synthesis of α-hydroxy ketones and vicinal diols is an intriguing field because of the broad applicability of these molecules. Although, butandiol dehydrogenases are known to play a key role in the production of 2,3-butandiol, their potential as biocatalysts is still not well studied. Here, we investigate the biocatalytic properties of the meso-butanediol dehydrogenase from Bacillus licheniformis DSM 13T (BlBDH). The encoding gene was cloned with an N-terminal StrepII-tag and recombinantly overexpressed in E. coli. BlBDH is highly active towards several non-physiological diketones and α-hydroxyketones with varying aliphatic chain lengths or even containing phenyl moieties. By adjusting the reaction parameters in biotransformations the formation of either the α-hydroxyketone intermediate or the diol can be controlled. Y1 - 2020 SN - 2590-1559 U6 - http://dx.doi.org/10.1016/j.jbiotec.2020.09.016 VL - 202 IS - Vol. 324 SP - 61 EP - 70 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Müller, Wolfram A1 - Jung, Alexander A1 - Ahammer, Helmut T1 - Advantages and problems of nonlinear methods applied to analyze physiological time signals: human balance control as an example JF - Scientific Reports Y1 - 2017 SN - 2045-2322 U6 - http://dx.doi.org/10.1038/s41598-017-02665-5 VL - 7 IS - Article number 2464 SP - 1 EP - 11 PB - Springer Nature CY - Cham ER -