TY  - JOUR
A1  - Zientz, Evelyn
A1  - Bongaerts, Johannes
A1  - Unden, Gottfried
T1  - Fumarate regulation of gene expression in Escherichia coli by the DcuSR (dcuSR genes) two-component regulatory system
JF  - Journal of bacteriology
Y1  - 1998
SN  - 1098-5530 (E-Journal); 0021-9193 (Print)
VL  - Vol. 180
IS  - No. 20
SP  - 5421
EP  - 5425
ER  - 
TY  - JOUR
A1  - Wissenbach, U.
A1  - Six, S.
A1  - Bongaerts, Johannes
A1  - Ternes, D.
A1  - Steinwachs, S.
A1  - Unden, G.
T1  - A third periplasmic transport system for l-arginine in Escherichia coli: molecular characterization of the artPIQMJ genes, arginine binding and transport
JF  - Molecular microbiology
Y1  - 1995
SN  - 1365-2958 (E-Journal); 0950-382x (Print)
VL  - Vol. 17
IS  - Iss. 4
SP  - 675
EP  - 686
ER  - 
TY  - JOUR
A1  - Wilming, Anja
A1  - Begemann, Jens
A1  - Kuhne, Stefan
A1  - Regestein, Lars
A1  - Bongaerts, Johannes
A1  - Evers, Stefan
A1  - Maurer, Karl-Heinz
A1  - Büchs, Jochen
T1  - Metabolic studies of γ-polyglutamic acid production in Bacillus licheniformis by small-scale continuous cultivations
JF  - Biochemical engineering journal
Y1  - 2013
SN  - 1873-295X (E-Journal); 1369-703X (Print)
VL  - Vol. 73
SP  - 29
EP  - 37
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Wiegand, Sandra
A1  - Voigt, Birgit
A1  - Albrecht, Dirk
A1  - Bongaerts, Johannes
A1  - Evers, Stefan
A1  - Hecker, Michael
A1  - Daniel, Rolf
A1  - Liesegang, Heiko
T1  - Fermentation stage-dependent adaptations of Bacillus licheniformis during enzyme production
JF  - Microbial Cell Factories
Y1  - 2013
U6  - http://dx.doi.org/10.1186/1475-2859-12-120
SN  - 1475-2859
VL  - 12
SP  - 120
PB  - Biomed Central
CY  - London
ER  - 
TY  - JOUR
A1  - Wiegand, Sandra
A1  - Dietrich, Sascha
A1  - Hertel, Robert
A1  - Bongaerts, Johannes
A1  - Evers, Stefan
A1  - Volland, Sonja
A1  - Daniel, Rolf
A1  - Liesegang, Heiko
T1  - RNA-Seq of Bacillus licheniformis: active regulatory RNA features expressed within a productive fermentation
JF  - BMC genomics
Y1  - 2013
SN  - 1471-2164
VL  - Vol. 14
SP  - 667
PB  - BioMed Central
CY  - London
ER  - 
TY  - JOUR
A1  - Welden, Melanie
A1  - Severins, Robin
A1  - Poghossian, Arshak
A1  - Wege, Christina
A1  - Bongaerts, Johannes
A1  - Siegert, Petra
A1  - Keusgen, Michael
A1  - Schöning, Michael Josef
T1  - Detection of acetoin and diacetyl by a tobacco mosaic virus-assisted field-effect biosensor
JF  - Chemosensors
N2  - Acetoin and diacetyl have a major impact on the flavor of alcoholic beverages such as wine or beer. Therefore, their measurement is important during the fermentation process. Until now, gas chromatographic techniques have typically been applied; however, these require expensive laboratory equipment and trained staff, and do not allow for online monitoring. In this work, a capacitive electrolyte–insulator–semiconductor sensor modified with tobacco mosaic virus (TMV) particles as enzyme nanocarriers for the detection of acetoin and diacetyl is presented. The enzyme acetoin reductase from Alkalihalobacillus clausii DSM 8716ᵀ is immobilized via biotin–streptavidin affinity, binding to the surface of the TMV particles. The TMV-assisted biosensor is electrochemically characterized by means of leakage–current, capacitance–voltage, and constant capacitance measurements. In this paper, the novel biosensor is studied regarding its sensitivity and long-term stability in buffer solution. Moreover, the TMV-assisted capacitive field-effect sensor is applied for the detection of diacetyl for the first time. The measurement of acetoin and diacetyl with the same sensor setup is demonstrated. Finally, the successive detection of acetoin and diacetyl in buffer and in diluted beer is studied by tuning the sensitivity of the biosensor using the pH value of the measurement solution.
Y1  - 2022
U6  - http://dx.doi.org/10.3390/chemosensors10060218
SN  - 2227-9040
N1  - This article belongs to the Special Issue "Nanostructured Devices for Biochemical Sensing"
VL  - 10
IS  - 6
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Wackwitz, B.
A1  - Bongaerts, Johannes
A1  - Goodman, S. D.
A1  - Unden, Gottfried
T1  - Growth phase-dependent regulation of nuoA-N expression in Escherichia coli K-12 by the Fis protein: upstream binding sites and bioenergetic significance
JF  - Molecular and general genetics : MGG
Y1  - 1999
SN  - 1617-4623 (E-Journal); 1617-4615 (Print)
VL  - Vol. 262
IS  - Iss. 4 - 5
SP  - 876
EP  - 883
ER  - 
TY  - JOUR
A1  - Voigt, Birgit
A1  - Schroeter, Rebecca
A1  - Jürgen, Britta
A1  - Albrecht, Dirk
A1  - Evers, Stefan
A1  - Bongaerts, Johannes
A1  - Maurer, Karl-Heinz
A1  - Schweder, Thomas
A1  - Hecker, Michael
T1  - The response of Bacillus licheniformis to heat and ethanol stress and the role of the SigB regulon
JF  - Proteomics
Y1  - 2013
SN  - 1615-9861 (E-Journal); 1615-9853 (Print)
VL  - Vol. 13
IS  - Iss. 14
SP  - 2140
EP  - 2146
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Voigt, Birgit
A1  - Albrecht, Dirk
A1  - Sievers, Susanne
A1  - Becher, Dörte
A1  - Bongaerts, Johannes
A1  - Evers, Stefan
A1  - Schweder, Thomas
A1  - Maurer, Karl-Heinz
A1  - Hecker, Michael
T1  - High-resolution proteome maps of Bacillus licheniformis cells growing in minimal medium
JF  - Proteomics
Y1  - 2015
U6  - http://dx.doi.org/10.1002/pmic.201400504
SN  - 1615-9861
VL  - 15
IS  - 15
SP  - 2629
EP  - 2633
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Unden, Gottfried
A1  - Bongaerts, Johannes
T1  - Alternative respiratory pathways of Escherichia coli: energetics and transcriptional regulation in response to electron acceptors
JF  - Biochimica et biophysica acta (BBA) - Bioenergetics
Y1  - 1997
SN  - 1879-2650 (E-Journal); 0005-2728 (Print)
VL  - Vol. 1320
IS  - Iss. 3
SP  - 217
EP  - 234
ER  - 
TY  - JOUR
A1  - Unden, G.
A1  - Becker, S.
A1  - Bongaerts, Johannes
A1  - Schirawski, J.
A1  - Six, S.
T1  - Oxygen regulated gene expression in facultatively anaerobic bacteria
JF  - Antonie van Leeuwenhoek
Y1  - 1994
SN  - 0003-6072 (Print) ; 1572-9699 (online)
VL  - Vol. 66
IS  - Iss. 1-3
SP  - 3
EP  - 22
ER  - 
TY  - JOUR
A1  - Unden, G.
A1  - Becker, S.
A1  - Bongaerts, Johannes
A1  - Holighaus, G.
A1  - Schirawski, J.
A1  - Six, S.
T1  - O2-sensing and O2-dependent gene regulation in facultatively anaerobic bacteria
JF  - Archives of microbiology
Y1  - 1995
SN  - 1432-072X (E-Journal); 0003-9276 (Print); 0302-8933 (Print)
VL  - Vol. 164
IS  - Iss. 2
SP  - 81
EP  - 90
ER  - 
TY  - JOUR
A1  - Tran, Quang Hon
A1  - Bongaerts, Johannes
A1  - Vlad, Dorina
A1  - Unden, Gottfried
T1  - Requirement for the proton-pumping NADH dehydrogenase I of Escherichia coli in respiration of NADH to fumarate and its bioenergetic implications
JF  - European journal of biochemistry
Y1  - 1997
SN  - 0014-2956
VL  - Vol. 244
IS  - Iss. 1
SP  - 155
EP  - 160
ER  - 
TY  - JOUR
A1  - Schroeter, Rebecca
A1  - Hoffmann, Tamara
A1  - Voigt, Birgit
A1  - Meyer, Hanna
A1  - Bleisteiner, Monika
A1  - Muntel, Jan
A1  - Jürgen, Britta
A1  - Albrecht, Dirk
A1  - Becher, Dörte
A1  - Lalk, Michael
A1  - Evers, Stefan
A1  - Bongaerts, Johannes
A1  - Maurer, Karl-Heinz
A1  - Putzer, Harald
A1  - Hecker, Michael
A1  - Schweder, Thomas
A1  - Bremer, Erhard
T1  - Stress responses of the industrial workhorse Bacillus licheniformis to osmotic challenges
JF  - PLoS ONE
N2  - The Gram-positive endospore-forming bacterium Bacillus licheniformis can be found widely in nature and it is exploited in industrial processes for the manufacturing of antibiotics, specialty chemicals, and enzymes. Both in its varied natural habitats and in industrial settings, B. licheniformis cells will be exposed to increases in the external osmolarity, conditions that trigger water efflux, impair turgor, cause the cessation of growth, and negatively affect the productivity of cell factories in biotechnological processes. We have taken here both systems-wide and targeted physiological approaches to unravel the core of the osmostress responses of B. licheniformis. Cells were suddenly subjected to an osmotic upshift of considerable magnitude (with 1 M NaCl), and their transcriptional profile was then recorded in a time-resolved fashion on a genome-wide scale. A bioinformatics cluster analysis was used to group the osmotically up-regulated genes into categories that are functionally associated with the synthesis and import of osmostress-relieving compounds (compatible solutes), the SigB-controlled general stress response, and genes whose functional annotation suggests that salt stress triggers secondary oxidative stress responses in B. licheniformis. The data set focusing on the transcriptional profile of B. licheniformis was enriched by proteomics aimed at identifying those proteins that were accumulated by the cells through increased biosynthesis in response to osmotic stress. Furthermore, these global approaches were augmented by a set of experiments that addressed the synthesis of the compatible solutes proline and glycine betaine and assessed the growth-enhancing effects of various osmoprotectants. Combined, our data provide a blueprint of the cellular adjustment processes of B. licheniformis to both sudden and sustained osmotic stress.
Y1  - 2014
U6  - http://dx.doi.org/10.1371/journal.pone.0080956
SN  - 1932-6203
VL  - 8
IS  - 11
PB  - PLOS
CY  - San Francisco
ER  - 
TY  - JOUR
A1  - Schmitz, M.
A1  - Hirsch, E.
A1  - Bongaerts, Johannes
A1  - Takors, Ralf
T1  - Pulse experiments as a prerequisite for the quantification of in vivo enzyme kinetics in aromatic amino acid pathway of Eschericia coli
JF  - Biotechnology progress
Y1  - 2002
SN  - 1520-6033 (E-Journal); 8756-7938 (Print)
VL  - Vol. 18
IS  - Iss. 5
SP  - 935
EP  - 941
ER  - 
TY  - JOUR
A1  - Scheele, Sandra
A1  - Oertel, Dan
A1  - Bongaerts, Johannes
A1  - Evers, Stefan
A1  - Hellmuth, Hendrik
A1  - Maurer, Karl-Heinz
A1  - Bott, Michael
A1  - Freudl, Roland
T1  - Secretory production of an FAD cofactor-containing cytosolic enzyme (sorbitol–xylitol oxidase from Streptomyces coelicolor) using the twin-arginine translocation (Tat) pathway of Corynebacterium glutamicum
JF  - Microbial biotechnology
Y1  - 2013
SN  - 1751-7915
SP  - 202
EP  - 206
PB  - Wiley-Blackwell
CY  - Oxford
ER  - 
TY  - JOUR
A1  - Scheele, S.
A1  - Bongaerts, Johannes
A1  - Maurer, K.-H.
A1  - Freudl, R.
T1  - Sekretion einer Kofaktor-haltigen Oxidase durch Corynebacterium glutamicum
JF  - Chemie - Ingenieur - Technik (CIT)
Y1  - 2009
SN  - 1522-2640 (E-Journal); 0009-286X (Print)
VL  - Vol. 81
IS  - Iss. 8
SP  - 1309
ER  - 
TY  - JOUR
A1  - Rachinger, Michael
A1  - Bauch, Melanie
A1  - Strittmatter, Axel
A1  - Bongaerts, Johannes
A1  - Evers, Stefan
A1  - Maurer, Karl-Heinz
A1  - Daniel, Rolf
A1  - Liebl, Wolfgang
A1  - Liesegang, Heiko
A1  - Ehrenreich, Armin
T1  - Size unlimited markerless deletions by a transconjugative plasmid-system in Bacillus licheniformis
JF  - Journal of biotechnology
Y1  - 2013
SN  - 1873-4863 (E-Journal); 0168-1656 (Print)
VL  - Vol. 164
IS  - Iss. 4
SP  - 365
EP  - 369
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Polen, T.
A1  - Krämer, Marco
A1  - Bongaerts, Johannes
A1  - Wubbolts, Marcel
A1  - Wendisch, V. F.
T1  - The global gene expression response of Escherichia coli to L-phenylalanine
JF  - Journal of biotechnology
Y1  - 2005
SN  - 1873-4863 (E-Journal); 0168-1656 (Print)
VL  - Vol. 115
IS  - Iss. 3
SP  - 221
EP  - 237
ER  - 
TY  - JOUR
A1  - Müller, Ulrike
A1  - Bongaerts, Johannes
A1  - Bovenberg, Roel
A1  - Jossek, Ralf
A1  - Krämer, Marco
A1  - Linnemann, J.
A1  - Müschen, S.
A1  - Ritterbecks, S.
A1  - Sprenger, G.
A1  - Wubbolts, Marcel
T1  - Metabolic engineering to produce fine chemicals in Escherichia coli
JF  - Mededelingen van de Faculteit Landbouwwetenschappen, Rijksuniversiteit Gent
Y1  - 2001
SN  - 0035-533x
VL  - 66 (3a)
SP  - 215
EP  - 217
ER  -