TY  - CHAP
A1  - Digel, Ilya
A1  - Dachwald, Bernd
A1  - Artmann, Gerhard
A1  - Linder, Peter
A1  - Funke, O.
T1  - A concept of a probe for particle analysis and life detection in icy environments
N2  - A melting probe equipped with autofluorescence-based detection system combined with a light scattering unit, and, optionally, with a microarray chip would be ideally suited to probe icy environments like Europa’s ice layer as well as the polar ice layers of Earth and Mars for recent and extinct live.
KW  - Sonde
KW  - Eisschicht
KW  - Autofluoreszenzverfahren
KW  - Lichtstreuungsbasierte Instrumente
KW  - autofluorescence-based detection system
KW  - light scattering analysis
Y1  - 2009
ER  - 
TY  - JOUR
A1  - Digel, Ilya
A1  - Dachwald, Bernd
A1  - Artmann, Gerhard
A1  - Linder, Peter
A1  - Funke, O.
T1  - A concept of a probe for particle analysis and life detection in icy environments
Y1  - 2009
N1  - International workshop “Europa lander: science goals and experiments”, Space Research Institute (IKI), Moscow, Russia
9-13 February 2009
SP  - 1
EP  - 24
ER  - 
TY  - CHAP
A1  - de Honde, Lukas
A1  - Porst, Dariusz
A1  - Digel, Ilya
ED  - Fischerauer, Alice
T1  - A randomized, observational thermographic study of the neck region before and after a physiotherapeutic intervention
T2  - 2nd YRA MedTech Symposium 2017 : June 8th - 9th / 2017 / Hochschule Ruhr-West
Y1  - 2017
SN  - 978-3-9814801-9-1
U6  - http://dx.doi.org/10.17185/duepublico/43984
N1  - A young researchers track of the 7th IEEE Workshop & SENSORICA 2017
SP  - 122
EP  - 123
PB  - Universität Duisburg-Essen
CY  - Duisburg
ER  - 
TY  - JOUR
A1  - Akimbekov, N.Sh.
A1  - Digel, Ilya
A1  - O’Heras, C.
A1  - Tastambek, K.T.
A1  - Savitskaya, I.S.
A1  - Ualyeva, P.S.
A1  - Mansurov, Z.A.
A1  - Zhubanova, A.A.
T1  - Adsorption of bacterial lipopol ysaccharides on carbonized ri ce husks obtained in the batch  experiments
JF  - KazNU Bulletin. Biology series
Y1  - 2015
SN  - 1563-0218
VL  - 60
IS  - No 1/2
SP  - 144
EP  - 148
ER  - 
TY  - JOUR
A1  - Zhubanova, Azhar A.
A1  - Aknazarov, S. K.
A1  - Mansurov, Zulkhair
A1  - Digel, Ilya
A1  - Kozhalakova, A. A.
A1  - Akimbekov, Nuraly Shardarbekovich
A1  - O'Heras, Carlos
A1  - Tazhibayeva, S.
A1  - Artmann, Gerhard
A1  - Temiz Artmann, Aysegül
T1  - Adsorption of bacterial Lipopolysaccharides and blood plasma proteins on modified carbonized materials
N2  - Bacterial lipopolysaccharides (endotoxins) show strong biological effects at very low concentrations in human beings and many animals when entering the blood stream. These include affecting structure and function of organs and cells, changing metabolic functions, raising body temperature, triggering the coagulation cascade, modifying hemodynamics and causing septic shock. Because of this toxicity, the removal of even minute amounts is essential for safe parenteral administration of drugs and also for septic shock patients' care. The absence of a general method for endotoxin removal from liquid interfaces urgently requires finding new methods and materials to overcome this gap. Nanostructured carbonized plant parts is a promising material that showed good adsorption properties due to its vast pore network and high surface area. The aim of this study was comparative measurement of endotoxin- and blood proteins-related adsorption rate and adsorption capacity for different carboneous materials produced at different temperatures and under different surface modifications. As a main surface modificator, positively cbarged polymer, polyethileneimine (PEl) was used. Activated carbon materials showed good adsorption properties for LPS and some proteins used in the experiments. During the batch experiments, several techniques (dust removal, autoclaving) were used and optimized for improving the material's adsorption behavior. Also, with the results obtained it was possible to differentiate the materials according to their adsorption capacity and kinetic characteristics. Modification of the surface apparently has not affected hemoglobin binding to the adsorbent's surface. Obtained adsorption isotherms can be used as a powerful tool for designing of future column-based setups for blood purification from LPS, which is especially important for septic shock treatment.
KW  - Kohlenstofffaser
KW  - Lipopolysaccharide
KW  - nanostrukturierte carbonisierte Pflanzenteile
KW  - lipopolysaccharides
KW  - nanostructured carbonized plant parts
Y1  - 2010
ER  - 
TY  - JOUR
A1  - Akimbekov, N.Sh.
A1  - Digel, Ilya
A1  - O´Heras, C.
A1  - Tastambek, K.T.
A1  - Savitskaya, I.S.
A1  - Ualyeva, P.S.
A1  - Mansurov, Z.A.
A1  - Zhubanova, A.A.
T1  - Adsorption of bacterial lipopolysaccharides on carbonized rice husks obtained in the batch experiments
JF  - Experimental Biology
N2  - The  scope  of  this  study  is  the  measurement  of  endotoxin  adsorption  rate  for  carbonized  rice  husk.  It  showed  good  adsorption properties for LPS. During the batch experiments, several techniques were used and optimized for improving the  material’s  adsorption  behavior.  Also,  with  the  results  obtained  it  was  possible  to  differentiate  the  materials  according to their adsorption capacity and kinetic characteristics.
KW  - surface modification
KW  - adsorption
KW  - carbonized rice husk
KW  - lipopolysaccharide
Y1  - 2014
SN  - 1563-0218
N1  - Original in russischer Sprache
VL  - 60
IS  - 1/2
SP  - 144
EP  - 148
PB  - Al-Farabi Kazakh National University
CY  - Almaty
ER  - 
TY  - JOUR
A1  - Kozhalakova, A. A.
A1  - Zhubanova, Azhar A.
A1  - Mansurov, Z. A.
A1  - Digel, Ilya
A1  - Tazhibayeva, S. M.
A1  - Artmann, Gerhard
A1  - Temiz Artmann, Aysegül
T1  - Adsorption of bacterial lipopolysaccharides on carbonized rice shell
JF  - Science of Central Asia (2010)
Y1  - 2010
SP  - 50
EP  - 54
ER  - 
TY  - JOUR
A1  - Bassam, Rasha
A1  - Artmann, Gerhard
A1  - Hescheler, Jürgen
A1  - Graef, T.
A1  - Temiz Artmann, Aysegül
A1  - Porst, Dariusz
A1  - Linder, Peter
A1  - Kayser, Peter
A1  - Arinkin, Vladimir
A1  - Gossmann, Matthias
A1  - Digel, Ilya
T1  - Alterations in human hemoglobin structure related to red blood cell storage
N2  - The importance of the availability of stored blood or blood cells, respectively, for urgent transfusion cannot be overestimated. Nowadays, blood storage becomes even more important since blood products are used for epidemiological studies, bio-technical research or banked for transfusion purposes. Thus blood samples must not only be processed, stored, and shipped to preserve their efficacy and safety, but also all parameters of storage must be recorded and reported for Quality Assurance. Therefore, blood banks and clinical research facilities are seeking more accurate, automated means for blood storage and blood processing.
KW  - Hämoglobin
KW  - Hämoglobinstruktur
KW  - Blutzellenlagerung
KW  - Hemoglobin structure
KW  - Red blood cell storage
Y1  - 2011
ER  - 
TY  - JOUR
A1  - Savitskaya, I.S.
A1  - Kistaubayeva, A.S.
A1  - Ignatova, L.V.
A1  - Digel, Ilya
T1  - Antimicrobial and wound healing properties of a bacterial cellulose based material containing B. subtilis cells
JF  - Heliyon
Y1  - 2019
U6  - http://dx.doi.org/10.1016/j.heliyon.2019.e02592
SN  - 2405-8440
VL  - 5
IS  - 10
SP  - Artikelnummer e02592
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Bogoyavlenskiy, A. P.
A1  - Digel, Ilya
A1  - Berezin, V. E.
T1  - Assessment of dot-blot ELISA sensitivity on membrane sorbent using various peroxidase substrates
N2  - The sensitivity of the peroxidase reaction in dot-blot ELISA significantly depends on the substrate. The highest sensitivity is observed using benzidine and diamine- phenol combinations as the substrates due to the reaction of the coupled oxidation (NADI)
KW  - Enzyme-linked immunosorbent assay
KW  - Peroxidase
KW  - ELISA
KW  - phenols
KW  - naphtols
KW  - aromatic amines
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Pogorelova, Natalia
A1  - Rogachev, Evgeniy
A1  - Digel, Ilya
A1  - Chernigova, Svetlana
A1  - Nardin, Dmitry
T1  - Bacterial Cellulose Nanocomposites: Morphology and Mechanical Properties
JF  - Materials
N2  - Bacterial cellulose (BC) is a promising material for biomedical applications due to its unique properties such as high mechanical strength and biocompatibility. This article describes the microbiological synthesis, modification, and characterization of the obtained BC-nanocomposites originating from symbiotic consortium Medusomyces gisevii. Two BC-modifications have been obtained: BC-Ag and BC-calcium phosphate (BC-Ca3(PO4)2). Structure and physicochemical properties of the BC and its modifications were investigated by scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), atomic force microscopy (AFM), and infrared Fourier spectroscopy as well as by measurements of mechanical and water holding/absorbing capacities. Topographic analysis of the surface revealed multicomponent thick fibrils (150–160 nm in diameter and about 15 µm in length) constituted by 50–60 nm nanofibrils weaved into a left-hand helix. Distinctive features of Ca-phosphate-modified BC samples were (a) the presence of 500–700 nm entanglements and (b) inclusions of Ca3(PO4)2 crystals. The samples impregnated with Ag nanoparticles exhibited numerous roundish inclusions, about 110 nm in diameter. The boundaries between the organic and inorganic phases were very distinct in both cases. The Ag-modified samples also showed a prominent waving pattern in the packing of nanofibrils. The obtained BC gel films possessed water-holding capacity of about 62.35 g/g. However, the dried (to a constant mass) BC-films later exhibited a low water absorption capacity (3.82 g/g). It was found that decellularized BC samples had 2.4 times larger Young’s modulus and 2.2 times greater tensile strength as compared to dehydrated native BC films. We presume that this was caused by molecular compaction of the BC structure.
Y1  - 2020
SN  - 1996-1944
U6  - http://dx.doi.org/10.3390/ma13122849
VL  - 13
IS  - 12
SP  - 1
EP  - 16
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Digel, Ilya
A1  - Akimbekov, Nuraly
A1  - Rogachev, Evgeniy
A1  - Pogorelova, Natalia
T1  - Bacterial cellulose produced by Medusomyces gisevii on glucose and sucrose: biosynthesis and structural properties
JF  - Cellulose
N2  - In this work, the effects of carbon sources and culture media on the production and structural properties of bacterial cellulose (BC) synthesized by Medusomyces gisevii have been studied. The culture medium was composed of different initial concentrations of glucose or sucrose dissolved in 0.4% extract of plain green tea. Parameters of the culture media (titratable acidity, substrate conversion degree etc.) were monitored daily for 20 days of cultivation. The BC pellicles produced on different carbon sources were characterized in terms of biomass yield, crystallinity and morphology by field emission scanning electron microscopy (FE-SEM), atomic force microscopy and X-ray diffraction. Our results showed that Medusomyces gisevii had higher BC yields in media with sugar concentrations close to 10 g L−1 after a 18–20 days incubation period. Glucose in general lead to a higher BC yield (173 g L−1) compared to sucrose (163.5 g L−1). The BC crystallinity degree and surface roughness were higher in the samples synthetized from sucrose. Obtained FE-SEM micrographs show that the BC pellicles synthesized in the sucrose media contained densely packed tangles of cellulose fibrils whereas the BC produced in the glucose media displayed rather linear geometry of the BC fibrils without noticeable aggregates.
KW  - Bacterial cellulose
KW  - Medusomyces gisevi
KW  - Carbon sources
KW  - Culture media
KW  - Cellulose nanostructure
Y1  - 2023
U6  - http://dx.doi.org/10.1007/s10570-023-05592-z
SN  - 1572-882X (Online)
SN  - 0969-0239 (Print)
N1  - Corresponding author: Ilya Digel
PB  - Springer Science + Business Media
CY  - Dordrecht
ER  - 
TY  - JOUR
A1  - Digel, Ilya
A1  - Temiz Artmann, Aysegül
A1  - Nishikawa, K.
A1  - Cook, M.
T1  - Bactericidal effects of plasma-generated cluster ions
JF  - Medical and Biological Engineering and Computing. 43 (2005), H. 6
Y1  - 2005
SN  - 1741-0444
SP  - 800
EP  - 807
ER  - 
TY  - JOUR
A1  - Mansurov, Z.
A1  - Digel, Ilya
A1  - Biisenbaev, M.
A1  - Savistkaya, I.
A1  - Kistaubaeva, A.
A1  - Akimbekov, N.
A1  - Zhubanova, A.
T1  - Bio-composite material on the basis of carbonized rice husk in biomedicine and environmental applications
JF  - Eurasian Chemico-Technological Journal
Y1  - 2012
U6  - http://dx.doi.org/10.18321/ectj105
SN  - 2522-4867
VL  - 14
IS  - 2
SP  - 115
EP  - 131
PB  - Institute of Combustion Problems
CY  - Almaty
ER  - 
TY  - JOUR
A1  - Akimbekov, N. S.
A1  - Digel, Ilya
A1  - Tastambek, K. T.
A1  - Zhubanova, A. A.
T1  - Biocompatibility of carbonized rice husk with a rat heart cells line H9c2
JF  - Experimental Biology
Y1  - 2013
SN  - 1563-0218
N1  - Original in russischer Sprache
VL  - 59
IS  - 3/1
SP  - 23
EP  - 25
ER  - 
TY  - CHAP
A1  - Mansurov, Zulkhair A.
A1  - Jandosov, Jakpar
A1  - Chenchik, D.
A1  - Azat, Seitkhan
A1  - Savitskaya, Irina S.
A1  - Kistaubaeva, Aida
A1  - Akimbekov, Nuraly
A1  - Digel, Ilya
A1  - Zhubanova, Azhar Achmet
T1  - Biocomposite Materials Based on Carbonized Rice Husk in Biomedicine and Environmental Applications
T2  - Carbon Nanomaterials in Biomedicine and the Environment
N2  - This chapter describes the prospects for biomedical and environmental engineering applications of heterogeneous materials based on nanostructured carbonized rice husk. Efforts in engineering enzymology are focused on the following directions: development and optimization of immobilization methods leading to novel biotechnological and biomedical applications; construction of biocomposite materials based on individual enzymes, multi-enzyme complexes and whole cells, targeted on realization of specific industrial processes. Molecular biological and biochemical studies on cell adhesion focus predominantly on identification, isolation and structural analysis of attachment-responsible biological molecules and their genetic determinants. The chapter provides a short overview of applications of the biocomposite materials based of nanostructured carbonized adsorbents. It emphasizes that further studies and better understanding of the interactions between CNS and microbial cells are necessary. The future use of living cells as biocatalysts, especially in the environmental field, needs more systematic investigations of the microbial adsorption phenomenon.
Y1  - 2020
SN  - 978-981-4800-27-3
U6  - http://dx.doi.org/10.1201/9780429428647-2
SP  - 3
EP  - 32
PB  - Jenny Stanford Publishing Pte. Ltd.
CY  - Singapore
ER  - 
TY  - BOOK
A1  - Artmann, Gerhard
A1  - Temiz Artmann, Aysegül
A1  - Zhubanova, Azhar A.
A1  - Digel, Ilya
ED  - Artmann, Gerhard
ED  - Temiz Artmann, Aysegül
ED  - Zhubanova, Azhar A.
ED  - Digel, Ilya
T1  - Biological, physical and technical basics of cell engineering
Y1  - 2018
SN  - 978-981-10-7903-0
PB  - Springer
CY  - Singapore
ER  - 
TY  - CHAP
A1  - Artmann, Gerhard
A1  - Digel, Ilya
A1  - Linder, Peter
A1  - Temiz Artmann, Aysegül
T1  - Biophysical and Engineering Contributions to Plant Research
N2  - Tests with palm tree leaves have just started yet and scan data are in the process to be analyzed. The final goal of future project for palm tree gender and species recognition will be to develop optical scanning technology to be applied to date palm tree leaves for in–situ screening purposes. Depending on the software used and the particular requirements of the users the technology potentially shall be able to identify palm tree diseases, palm tree gender, and species of young date palm trees by scanning leaves.
KW  - Pflanzenphysiologie
KW  - Dattel
KW  - Pflanzenscanner
KW  - plant scanner
KW  - date palm tree
Y1  - 2011
ER  - 
TY  - JOUR
A1  - Akimbekov, Nuraly S.
A1  - Digel, Ilya
A1  - Tastambek, Kuanysh T.
A1  - Marat, Adel K.
A1  - Turaliyeva, Moldir A.
A1  - Kaiyrmanova, Gulzhan K.
T1  - Biotechnology of Microorganisms from Coal Environments: From Environmental Remediation to Energy Production
JF  - Biology
N2  - It was generally believed that coal sources are not favorable as live-in habitats for microorganisms due to their recalcitrant chemical nature and negligible decomposition. However, accumulating evidence has revealed the presence of diverse microbial groups in coal environments and their significant metabolic role in coal biogeochemical dynamics and ecosystem functioning. The high oxygen content, organic fractions, and lignin-like structures of lower-rank coals may provide effective means for microbial attack, still representing a greatly unexplored frontier in microbiology. Coal degradation/conversion technology by native bacterial and fungal species has great potential in agricultural development, chemical industry production, and environmental rehabilitation. Furthermore, native microalgal species can offer a sustainable energy source and an excellent bioremediation strategy applicable to coal spill/seam waters. Additionally, the measures of the fate of the microbial community would serve as an indicator of restoration progress on post-coal-mining sites. This review puts forward a comprehensive vision of coal biodegradation and bioprocessing by microorganisms native to coal environments for determining their biotechnological potential and possible applications.
Y1  - 2022
U6  - http://dx.doi.org/10.3390/biology11091306
SN  - 2079-7737
N1  - This article belongs to the Special Issue "Microbial Ecology and Evolution in Extreme Environments"
VL  - 11
IS  - 9
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Digel, Ilya
A1  - Maggakis-Kelemen, Christina
A1  - Zerlin, Kay
A1  - Linder, Peter
T1  - Body temperature-related structural transitions of monotremal and human hemoglobin
JF  - Biophysical Journal. 91 (2006), H. 8
Y1  - 2006
SN  - 1542-0086
SP  - 3014
EP  - 3021
ER  -