TY  - JOUR
A1  - Molinnus, Denise
A1  - Sorich, Maren
A1  - Bartz, Alexander
A1  - Siegert, Petra
A1  - Willenberg, Holger S.
A1  - Lisdat, Fred
A1  - Poghossian, Arshak
A1  - Keusgen, Michael
A1  - Schöning, Michael Josef
T1  - Towards an adrenaline biosensor based on substrate recycling amplification in combination with an enzyme logic gate
JF  - Sensors and Actuators B: Chemical
N2  - An amperometric biosensor using a substrate recycling principle was realized for the detection of low adrenaline concentrations (1 nM) by measurements in phosphate buffer and Ringer’s solution at pH 6.5 and pH 7.4, respectively. In proof-of-concept experiments, a Boolean logic-gate principle has been applied to develop a digital adrenaline biosensor based on an enzyme AND logic gate. The obtained results demonstrate that the developed digital biosensor is capable for a rapid qualitative determination of the presence/absence of adrenaline in a YES/NO statement. Such digital biosensor could be used in clinical diagnostics for the control of a correct insertion of a catheter in the adrenal veins during adrenal venous-sampling procedure.
Y1  - 2016
U6  - http://dx.doi.org/10.1016/j.snb.2016.06.064
SN  - 0925-4005
VL  - 237
SP  - 190
EP  - 195
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Berretz, F.
A1  - Skorupa, S.
A1  - Sander, Volker
A1  - Belloum, A.
T1  - Towards an actor-driven workflow management system for grids
JF  - International Symposium on Collaborative Technologies and Systems (CTS), 2010 : 17 - 21 May 2010, Chicago, Illinois, USA / IEEE
Y1  - 2010
SN  - 978-1-4244-6619-1
SP  - 611
EP  - 616
PB  - IEEE
CY  - Piscataway, NJ
ER  - 
TY  - JOUR
A1  - Poghossian, Arshak
A1  - Werner, Frederik
A1  - Buniatyan, V. V.
A1  - Wagner, Torsten
A1  - Miamoto, K.
A1  - Yoshinobu, T.
A1  - Schöning, Michael Josef
T1  - Towards addressability of light-addressable potentiometric sensors: Shunting effect of non-illuminated region and cross-talk
JF  - Sensor and Actuators B: Chemical
N2  - The LAPS (light-addressable potentiometric sensor) platform is one of the most attractive approaches for chemical and biological sensing with many applications ranging from pH and ion/analyte concentration measurements up to cell metabolism detection and chemical imaging. However, although it is generally accepted that LAPS measurements are spatially resolved, the light-addressability feature of LAPS devices has not been discussed in detail so far. In this work, an extended electrical equivalent-circuit model of the LAPS has been presented, which takes into account possible cross-talk effects due to the capacitive coupling of the non-illuminated region. A shunting effect of the non-illuminated area on the measured photocurrent and addressability of LAPS devices has been studied. It has been shown, that the measured photocurrent will be determined not only by the local interfacial potential in the illuminated region but also by possible interfacial potential changes in the non-illuminated region, yielding cross-talk effects. These findings were supported by the experimental investigations of a penicillin-sensitive multi-spot LAPS and a metal-insulator-semiconductor LAPS as model systems.
Y1  - 2017
U6  - http://dx.doi.org/10.1016/j.snb.2017.01.047
SN  - 0925-4005
IS  - 244
SP  - 1071
EP  - 1079
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Kirchner, Patrick
A1  - Oberländer, Jan
A1  - Suso, Henri-Pierre
A1  - Rysstad, Gunnar
A1  - Keusgen, Michael
A1  - Schöning, Michael Josef
T1  - Towards a wireless sensor system for real-time H2O2 monitoring in aseptic food processes
JF  - Physica status solidi (a)
N2  - A wireless sensor system based on the industrial ZigBee standard for low-rate wireless networking was developed that enables real-time monitoring of gaseous H2O2 during the package sterilization in aseptic food processes. The sensor system consists of a remote unit connected to a calorimetric gas sensor, which was already established in former works, and an external base unit connected to a laptop computer. The remote unit was built up by an XBee radio frequency (RF) module for data communication and a programmable system-on-chip controller to read out the sensor signal and process the sensor data, whereas the base unit is a second XBee RF module. For the rapid H2O2 detection on various locations inside the package that has to be sterilized, a novel read-out strategy of the calorimetric gas sensor was established, wherein the sensor response is measured within the short sterilization time and correlated with the present H2O2 concentration. In an exemplary measurement application in an aseptic filling machinery, the suitability of the new, wireless sensor system was demonstrated, wherein the influence of the gas velocity on the H2O2 distribution inside a package was determined and verified with microbiological tests.
KW  - calorimetric gas sensor;hydrogen peroxide;wireless sensor system
Y1  - 2013
U6  - http://dx.doi.org/10.1002/pssa.201200920
SN  - 1862-6319
VL  - 210
IS  - 5
SP  - 877
EP  - 883
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Gerhards, M.
A1  - Skorupa, S.
A1  - Sander, Volker
A1  - Pfeiffer, P.
A1  - Belloum, A.
T1  - Towards a security framework for a WS-HumanTask processor
JF  - 7th International Conference on  Network and Service Management (CNSM), 2011 Paris
Y1  - 2011
SN  - 978-3-901882-44-9
SP  - 1
EP  - 5
PB  - IEEE
CY  - New York
ER  - 
TY  - JOUR
A1  - Reisert, Steffen
A1  - Geissler, Hanno
A1  - Flörke, Rudolf
A1  - Näther, Niko
A1  - Wagner, Patrick
A1  - Schöning, Michael Josef
T1  - Towards a multi-sensor system for the evaluation of aseptic processes employing hydrogen peroxide vapour (H2O2)
JF  - Physica status solidi (a) : applications and material science. 208 (2011), H. 6
Y1  - 2011
SN  - 1862-6319
SP  - 1351
EP  - 1356
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Molinnus, Denise
A1  - Beging, Stefan
A1  - Lowis, Carsten
A1  - Schöning, Michael Josef
T1  - Towards a multi-enzyme capacitive field-effect biosensor by comparative study of drop-coating and nano-spotting technique
JF  - Sensors
N2  - Multi-enzyme immobilization onto a capacitive field-effect biosensor by nano-spotting technique is presented. The nano-spotting technique allows to immobilize different enzymes simultaneously on the sensor surface with high spatial resolution without additional photolithographical patterning. The amount of applied enzymatic cocktail on the sensor surface can be tailored. Capacitive electrolyte-insulator-semiconductor (EIS) field-effect sensors with Ta2O5 as pH-sensitive transducer layer have been chosen to immobilize the three different (pL droplets) enzymes penicillinase, urease, and glucose oxidase. Nano-spotting immobilization is compared to conventional drop-coating method by defining different geometrical layouts on the sensor surface (fully, half-, and quarter-spotted). The drop diameter is varying between 84 µm and 102 µm, depending on the number of applied drops (1 to 4) per spot. For multi-analyte detection, penicillinase and urease are simultaneously nano-spotted on the EIS sensor. Sensor characterization was performed by C/V (capacitance/voltage) and ConCap (constant capacitance) measurements. Average penicillin, glucose, and urea sensitivities for the spotted enzymes were 81.7 mV/dec, 40.5 mV/dec, and 68.9 mV/dec, respectively.
Y1  - 2020
SN  - 1424-8220
U6  - http://dx.doi.org/10.3390/s20174924
N1  - Special issue: Multisensor Systems and Signal Processing in Analytical Chemistry
VL  - 20
IS  - 17
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Ferrein, Alexander
A1  - Dylla, Frank
A1  - Lakemeyer, Gerhard
A1  - Murray, Jan
T1  - Towards a League-Independent Qualitative Soccer Theory for RoboCup  / Dylla, Frank ; Ferrein, Alexander ; Lakemeyer, Gerhard ; Murray, Jan ; Obst, Oliver ; Röfer, Thomas ; Stolzenburg, Frieder ; Visser, Ubbo
JF  - RoboCup 2004: Robot Soccer World Cup VIII / Daniele Nardi ... (ed.).
Y1  - 2005
SN  - 978-3-540-25046-8
N1  - Lecture Notes in Computer Science ; 3276
SP  - 611
EP  - 618
PB  - Springer
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Molinnus, Denise
A1  - Drinic, Aleksander
A1  - Iken, Heiko
A1  - Kröger, Nadja
A1  - Zinser, Max
A1  - Smeets, Ralf
A1  - Köpf, Marius
A1  - Kopp, Alexander
A1  - Schöning, Michael Josef
T1  - Towards a flexible electrochemical biosensor fabricated from biocompatible Bombyx mori silk
JF  - Biosensors and Bioelectronics
Y1  - 2021
U6  - http://dx.doi.org/10.1016/j.bios.2021.113204
SN  - 0956-5663
VL  - 183
IS  - Art. 113204
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Arzdorf, M.
A1  - Mulchandani, P.
A1  - Chen, W.
A1  - Mulchandani, A.
T1  - Towards a capacitive enzyme sensor for direct determination of organophosphorus pesticides: Fundamentals studies and aspects of development
JF  - Sensors. 3 (2003), H. 6
Y1  - 2003
SN  - 1424-8220
SP  - 119
EP  - 127
ER  - 
TY  - JOUR
A1  - Oberländer, Jan
A1  - Bromm, Alexander
A1  - Wendeler, Luisa
A1  - Iken, Heiko
A1  - Palomar Duran, Marlena
A1  - Greeff, Anton
A1  - Kirchner, Patrick
A1  - Keusgen, Michael
A1  - Schöning, Michael Josef
T1  - Towards a biosensor to monitor the sterilisation efficiency of aseptic filling machines
JF  - Physica status solidi (a)
N2  - Sterilisation processes are compulsory in medicine, pharmacy, and food industries to prevent infections of consumers and microbiological contaminations of products. Monitoring the sterilisation by conventional microbiological methods is time- and lab-consuming. To overcome this problem, in this work a novel biosensor has been proposed. The sensor enables a fast method to evaluate sterilisation processes. By means of thin-film technology the sensor's transducer structures in form of IDEs (interdigitated electrodes) have been fabricated on a silicon substrate. Physical characterisation of the developed sensor was done by AFM, SEM, and profilometry. Impedance analyses were conducted for the electrical characterisation. As microbiological layer spores of B. atrophaeus have been immobilised on the sensing structure; spores of this type are a well-known sterilisation test organism. Impedance measurements at a fixed frequency over time were performed to monitor the immobilisation process. A sterilisation process according to aseptic filling machines was applied to demonstrate the sensor functionality. After both, immobilisation and sterilisation, a change in impedance could successfully be detected.
Y1  - 2015
U6  - http://dx.doi.org/10.1002/pssa.201431900
SN  - 1862-6319
VL  - 212
IS  - 6
SP  - 1299
EP  - 1305
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Takenaga, Shoko
A1  - Biselli, Manfred
A1  - Schnitzler, Thomas
A1  - Öhlschläger, Peter
A1  - Wagner, Torsten
A1  - Schöning, Michael Josef
T1  - Toward multi-analyte bioarray sensors: LAPS-based on-chip determination of a Michaelis–Menten-like kinetics for cell culturing
JF  - Physica status solidi A : Applications and materials science
N2  - The metabolic activity of Chinese hamster ovary (CHO) cells was observed using a light-addressable potentiometric sensor (LAPS). The dependency toward different glucose concentrations (17–200 mM) follows a Michaelis–Menten kinetics trajectory with Kₘ = 32.8 mM, and the obtained Kₘ value in this experiment was compared with that found in literature. In addition, the pH shift induced by glucose metabolism of tumor cells transfected with the HPV-16 genome (C3 cells) was successfully observed. These results indicate the possibility to determine the tumor cells metabolism with a LAPS-based measurement device.
Y1  - 2014
U6  - http://dx.doi.org/10.1002/pssa.201330464
SN  - 1521-396X (E); 1862-6319 (E-Journal); 0031-8965 (Print); 1862-6300 (Print)
VL  - 211
IS  - 6
SP  - 1410
EP  - 1415
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Arreola, Julio
A1  - Keusgen, Michael
A1  - Schöning, Michael Josef
T1  - Toward an immobilization method for spore-based biosensors in oxidative environment
JF  - Electrochimica Acta
Y1  - 2019
U6  - http://dx.doi.org/10.1016/j.electacta.2019.01.148
VL  - 302
SP  - 394
EP  - 401
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Röhlen, Desiree
A1  - Pilas, Johanna
A1  - Dahmen, Markus
A1  - Keusgen, Michael
A1  - Selmer, Thorsten
A1  - Schöning, Michael Josef
T1  - Toward a Hybrid Biosensor System for Analysis of Organic and Volatile Fatty Acids in Fermentation Processes
JF  - Frontiers in Chemistry
N2  - Monitoring of organic acids (OA) and volatile fatty acids (VFA) is crucial for the control of anaerobic digestion. In case of unstable process conditions, an accumulation of these intermediates occurs. In the present work, two different enzyme-based biosensor arrays are combined and presented for facile electrochemical determination of several process-relevant analytes. Each biosensor utilizes a platinum sensor chip (14 × 14 mm²) with five individual working electrodes. The OA biosensor enables simultaneous measurement of ethanol, formate, d- and l-lactate, based on a bi-enzymatic detection principle. The second VFA biosensor provides an amperometric platform for quantification of acetate and propionate, mediated by oxidation of hydrogen peroxide. The cross-sensitivity of both biosensors toward potential interferents, typically present in fermentation samples, was investigated. The potential for practical application in complex media was successfully demonstrated in spiked sludge samples collected from three different biogas plants. Thereby, the results obtained by both of the biosensors were in good agreement to the applied reference measurements by photometry and gas chromatography, respectively. The proposed hybrid biosensor system was also used for long-term monitoring of a lab-scale biogas reactor (0.01 m³) for a period of 2 months. In combination with typically monitored parameters, such as gas quality, pH and FOS/TAC (volatile organic acids/total anorganic carbonate), the amperometric measurements of OA and VFA concentration could enhance the understanding of ongoing fermentation processes.
Y1  - 2018
U6  - http://dx.doi.org/10.3389/fchem.2018.00284
IS  - 6
PB  - Frontiers
CY  - Lausanne
ER  - 
TY  - JOUR
A1  - Fischer, Jan-Thomas
A1  - Kowalski, Julia
A1  - Pudasaini, Shiva P.
T1  - Topographic curvature effects in applied avalanche modelling
JF  - Cold Regions Science and Technology
N2  - This paper describes the implementation of topographic curvature effects within the RApid Mass MovementS (RAMMS) snow avalanche simulation toolbox. RAMMS is based on a model similar to shallow water equations with a Coulomb friction relation and the velocity dependent Voellmy drag. It is used for snow avalanche risk assessment in Switzerland. The snow avalanche simulation relies on back calculation of observed avalanches. The calibration of the friction parameters depends on characteristics of the avalanche track. The topographic curvature terms are not yet included in the above mentioned classical model. Here, we fundamentally improve this model by mathematically and physically including the topographic curvature effects. By decomposing the velocity dependent friction into a topography dependent term that accounts for a curvature enhancement in the Coulomb friction, and a topography independent contribution similar to the classical Voellmy drag, we construct a general curvature dependent frictional resistance, and thus propose new extended model equations. With three site-specific examples, we compare the apparent frictional resistance of the new approach, which includes topographic curvature effects, to the classical one. Our simulation results demonstrate substantial effects of the curvature on the flow dynamics e.g., the dynamic pressure distribution along the slope. The comparison of resistance coefficients between the two models demonstrates that the physically based extension presents an improvement to the classical approach. Furthermore a practical example highlights its influence on the pressure outline in the run out zone of the avalanche. Snow avalanche dynamics modeling natural terrain curvature centrifugal force friction coefficients.
KW  - Snow
KW  - Avalanche
Y1  - 2012
U6  - http://dx.doi.org/10.1016/j.coldregions.2012.01.005
SN  - 1872-7441
VL  - 74-75
SP  - 21
EP  - 30
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Augenstein, Eckardt
A1  - Herbergs, S.
A1  - Kuperjans, Isabel
T1  - TOP-Energy : ein Werkzeug zur Optimierung der Gebäudeenergieversorgung
JF  - KI : Kälte, Luft, Klimatechnik
Y1  - 2006
SN  - 1865-5432
IS  - 5
SP  - 198
EP  - 201
ER  - 
TY  - JOUR
A1  - Hirschfeld, J. A.
A1  - Lustfeld, H.
A1  - Reißel, Martin
A1  - Steffen, B.
T1  - Tomographic diagnostics of current distributions in a fuel cell stack
JF  - International Journal of Energy Research
Y1  - 2010
SN  - 0363-907X
VL  - 34
IS  - 3
SP  - 284
EP  - 292
ER  - 
TY  - JOUR
A1  - Bäcker, Matthias
A1  - Koch, Claudia
A1  - Eiben, Sabine
A1  - Geiger, Fania
A1  - Eber, Fabian
A1  - Gliemann, Hartmut
A1  - Poghossian, Arshak
A1  - Wege, Christina
A1  - Schöning, Michael Josef
T1  - Tobacco mosaic virus as enzyme nanocarrier for electrochemical biosensors
JF  - Sensors and Actuators B: Chemical
N2  - The conjunction of (bio-)chemical recognition elements with nanoscale biological building blocks such as virus particles is considered as a very promising strategy for the creation of biohybrids opening novel opportunities for label-free biosensing. This work presents a new approach for the development of biosensors using tobacco mosaic virus (TMV) nanotubes or coat proteins (CPs) as enzyme nanocarriers. Sensor chips combining an array of Pt electrodes loaded with glucose oxidase (GOD)-modified TMV nanotubes or CP aggregates were used for amperometric detection of glucose as a model system for the first time. The presence of TMV nanotubes or CPs on the sensor surface allows binding of a high amount of precisely positioned enzymes without substantial loss of their activity, and may also ensure accessibility of their active centers for analyte molecules. Specific and efficient immobilization of streptavidin-conjugated GOD ([SA]-GOD) complexes on biotinylated TMV nanotubes or CPs was achieved via bioaffinity binding. These layouts were tested in parallel with glucose sensors with adsorptively immobilized [SA]-GOD, as well as [SA]-GOD crosslinked with glutardialdehyde, and came out to exhibit superior sensor performance. The achieved results underline a great potential of an integration of virus/biomolecule hybrids with electronic transducers for future applications in biosensorics and biochips.
Y1  - 2017
U6  - http://dx.doi.org/10.1016/j.snb.2016.07.096
SN  - 0925-4005
VL  - 238
SP  - 716
EP  - 722
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Immel, Timo A.
A1  - Groth, Ulrich
A1  - Huhn, Thomas
A1  - Öhlschläger, Peter
T1  - Titanium salan complexes displays strong antitumor properties in vitro and in vivo in mice
JF  - PLoS ONE
N2  - The anticancer activity of titanium complexes has been known since the groundbreaking studies of Köpf and Köpf-Maier on titanocen dichloride. Unfortunately, possibly due to their fast hydrolysis, derivatives of titanocen dichloride failed in clinical studies. Recently, the new family of titanium salan complexes containing tetradentate ONNO ligands with anti-cancer properties has been discovered. These salan complexes are much more stabile in aqueous media. In this study we describe the biological activity of two titanium salan complexes in a mouse model of cervical cancer. High efficiency of this promising complex family was demonstrated for the first time in vivo. From these data we conclude that titanium salan complexes display very strong antitumor properties exhibiting only minor side effects. Our results may influence the chemotherapy with metallo therapeutics in the future.
Y1  - 2011
U6  - http://dx.doi.org/10.1371/journal.pone.0017869
VL  - 6
IS  - 3
PB  - Plos
CY  - San Francisco, California, US
ER  - 
TY  - JOUR
A1  - Hugenroth, Kristin
A1  - Neidlin, Michael
A1  - Engelmann, Ulrich M.
A1  - Kaufmann, Tim A. S.
A1  - Steinseifer, Ulrich
A1  - Heilmann, Torsten
T1  - Tipless Transseptal Cannula Concept Combines Improved Hemodynamic Properties and Risk‐Reduced Placement: an In Silico Proof‐of‐Concept
JF  - Artificial Organs
Y1  - 2021
U6  - http://dx.doi.org/10.1111/aor.13964
SN  - 1525-1594
IS  - Accepted Article
PB  - Wiley
CY  - Weinheim
ER  -