TY - JOUR A1 - Schiffer, Stefan A1 - Ferrein, Alexander A1 - Lakemeyer, Gerhard T1 - Proceedings of the Fourth International Conference on Intelligent Robotics and Applications (ICIRA 2011) JF - Proceedings of the Fourth International Conference on Intelligent Robotics and Applications (ICIRA 2011) Y1 - 2010 SP - 1 EP - 10 ER - TY - JOUR A1 - Scheer, Nico A1 - Ross, Jillian A1 - Kapelyukh, Yury A1 - Rode, Anja A1 - Wolf, C. Roland T1 - In vivo responses of the human and murine pregnane X receptor to dexamethasone in mice JF - Drug Metabolism and Disposition N2 - Dexamethasone (DEX) is a potent and widely used anti-inflammatory and immunosuppressant glucocorticoid. It can bind and activate the pregnane X receptor (PXR), which plays a critical role as xenobiotic sensor in mammals to induce the expression of many enzymes, including cytochromes P450 in the CYP3A family. This induction results in its own metabolism. We have used a series of transgenic mouse lines, including a novel, improved humanized PXR line, to compare the induction profile of PXR-regulated drug-metabolizing enzymes after DEX administration, as well as looking at hepatic responses to rifampicin (RIF). The new humanized PXR model has uncovered further intriguing differences between the human and mouse receptors in that RIF only induced Cyp2b10 in the new humanized model. DEX was found to be a much more potent inducer of Cyp3a proteins in wild-type mice than in mice humanized for PXR. To assess whether PXR is involved in the detoxification of DEX in the liver, we analyzed the consequences of high doses of the glucocorticoid on hepatotoxicity on different PXR genetic backgrounds. We also studied these effects in an additional mouse model in which functional mouse Cyp3a genes have been deleted. These strains exhibited different sensitivities to DEX, indicating a protective role of the PXR and CYP3A proteins against the hepatotoxicity of this compound. Y1 - 2010 U6 - https://doi.org/10.1124/dmd.109.031872 SN - 1521-009X VL - 38 IS - 7 SP - 1046 EP - 1053 PB - ASPET CY - Bethesda ER - TY - JOUR A1 - Ross, Jillian A1 - Plummer, Simon M. A1 - Rode, Anja A1 - Scheer, Nico A1 - Bower, Conrad C. A1 - Vogel, Ortwin A1 - Henderson, Colin J. A1 - Wolf, C. Roland A1 - Elcombe, Clifford R. T1 - Human constitutive androstane receptor (CAR) and pregnane X receptor (PXR) support the hypertrophic but not the hyperplastic response to the murine nongenotoxic hepatocarcinogens phenobarbital and chlordane in vivo JF - Toxicological Sciences N2 - Mouse nongenotoxic hepatocarcinogens phenobarbital (PB) and chlordane induce hepatomegaly characterized by hypertrophy and hyperplasia. Increased cell proliferation is implicated in the mechanism of tumor induction. The relevance of these tumors to human health is unclear. The xenoreceptors, constitutive androstane receptors (CARs), and pregnane X receptor (PXR) play key roles in these processes. Novel “humanized” and knockout models for both receptors were developed to investigate potential species differences in hepatomegaly. The effects of PB (80 mg/kg/4 days) and chlordane (10 mg/kg/4 days) were investigated in double humanized PXR and CAR (huPXR/huCAR), double knockout PXR and CAR (PXRKO/CARKO), and wild-type (WT) C57BL/6J mice. In WT mice, both compounds caused increased liver weight, hepatocellular hypertrophy, and cell proliferation. Both compounds caused alterations to a number of cell cycle genes consistent with induction of cell proliferation in WT mice. However, these gene expression changes did not occur in PXRKO/CARKO or huPXR/huCAR mice. Liver hypertrophy without hyperplasia was demonstrated in the huPXR/huCAR animals in response to both compounds. Induction of the CAR and PXR target genes, Cyp2b10 and Cyp3a11, was observed in both WT and huPXR/huCAR mouse lines following treatment with PB or chlordane. In the PXRKO/CARKO mice, neither liver growth nor induction of Cyp2b10 and Cyp3a11 was seen following PB or chlordane treatment, indicating that these effects are CAR/PXR dependent. These data suggest that the human receptors are able to support the chemically induced hypertrophic responses but not the hyperplastic (cell proliferation) responses. At this time, we cannot be certain that hCAR and hPXR when expressed in the mouse can function exactly as the genes do when they are expressed in human cells. However, all parameters investigated to date suggest that much of their functionality is maintained. Y1 - 2010 U6 - https://doi.org/10.1093/toxsci/kfq118 SN - 1096-0929 VL - 116 IS - 2 SP - 452 EP - 466 PB - Oxford University Press CY - Oxford ER - TY - JOUR A1 - Rigling, Andreas A1 - Eilmann, Britta A1 - Koechli, Roger A1 - Dobbertin, Matthias T1 - Mistletoe-induced crown degradation in Scots pine in a xeric environment N2 - Increasing Scots pine (Pinus sylvestris L.) mortality has been recently observed in the dry inner valleys of the European Alps. Besides drought, infection with pine mistletoe (Viscum album ssp. austriacum) seems to play an important role in the mortality dynamics of Scots pines, but how mistletoes promote pine decline remains unclear. To verify whether pine mistletoe infection weakens the host via crown degradation, as observed for dwarf mistletoes, we studied the negative effects of pine mistletoe infestation on the photosynthetic tissues and branch growth of pairs of infested and non-infested branches. Pine mistletoe infection leads to crown degradation in its host by reducing the length, the radial increment, the ramification, the needle length and the number of needle years of the infested branches. This massive loss in photosynthetic tissue results in a reduction in primary production and a subsequent decrease in carbohydrate availability. The significant reduction in needle length due to mistletoe infection is an indication for a lower water and nutrient availability in infested branches. Thus, mistletoe infection might lead to a decrease in the availability of water and carbohydrates, the two most important growth factors, which are already shortened due to the chronic drought situation in the area. Therefore, pine mistletoe increases the risk of drought-induced mortality of its host when growing in a xeric environment. Y1 - 2010 U6 - https://doi.org/10.1093/treephys/tpq038 SN - 1758-4469 (Online) SN - 0829-318X (Print) VL - 30 IS - 7 SP - 845 EP - 832 PB - Oxford University Press CY - Oxford ER - TY - JOUR A1 - Ribitsch, D. A1 - Karl, W. A1 - Birner-Gruenberger, R. A1 - Gruber, K. A1 - Eiteljoerg, I. A1 - Remler, P. A1 - Wieland, S. A1 - Siegert, Petra A1 - Maurer, Karl-Heinz A1 - Schwab, H. T1 - C-terminal truncation of a metagenome-derived detergent protease for effective expression in E. coli JF - Journal of biotechnology N2 - Recently, a new alkaline protease named HP70 showing highest homology to extracellular serine proteases of Stenotrophomonas maltophilia and Xanthomonas campestris was found in the course of a metagenome screening for detergent proteases (Niehaus et al., submitted for publication). Attempts to efficiently express the enzyme in common expression hosts had failed. This study reports on the realization of overexpression in Escherichia coli after structural modification of HP70. Modelling of HP70 resulted in a two-domain structure, comprising the catalytic domain and a C-terminal domain which includes about 100 amino acids. On the basis of the modelled structure the enzyme was truncated by deletion of most of the C-terminal domain yielding HP70-C477. This structural modification allowed effective expression of active enzyme using E. coli BL21-Gold as the host. Specific activity of HP70-C477 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 30 ± 5 U/mg compared to 8 ± 1 U/mg of the native enzyme. HP70-C477 was most active at 40 °C and pH 7–11; these conditions are prerequisite for a potential application as detergent enzyme. Determination of kinetic parameters at 40 °C and pH = 9.5 resulted in KM = 0.23 ± 0.01 mM and kcat = 167.5 ± 3.6 s⁻¹. MS-analysis of peptide fragments obtained from incubation of HP70 and HP70-C477 with insulin B indicated that the C-terminal domain influences the cleavage preferences of the enzyme. Washing experiments confirmed the high potential of HP70-C477 as detergent protease. Y1 - 2010 U6 - https://doi.org/10.1016/j.jbiotec.2010.09.947 SN - 1873-4863 (E-Journal); 0168-1656 (Print) VL - 150 IS - 3 SP - 408 EP - 416 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Rens, Gavin A1 - Varzinczak, Ivan A1 - Meyer, Thomas A1 - Ferrein, Alexander T1 - A Logic for Reasoning about Actions and Explicit Observations JF - AI 2010: Advances in Artificial Intelligence 23rd Australasian Joint Conference, Adelaide, Australia, December 7-10, 2010. Proceedings Y1 - 2010 SN - 978-3-642-17431-5 N1 - Lecture Notes in Computer Science ; 6464 SP - 395 EP - 404 PB - Springer CY - Berlin ER - TY - JOUR A1 - Reisgen, Uwe A1 - Schleser, Markus A1 - Mokrov, Oleg A1 - Ahmed, Essam T1 - Uni- and bi-axial deformation behavior of laser welded advanced high strength steel sheets JF - Journal of materials processing technology N2 - Bead-on-plate butt joints of 2.5 mm hot rolled DP600/DP600 and 1.2 mm cold rolled TRIP700/TRIP700 steel sheets were performed using 6 kW CO2 laser beam welding. The welding speed ranged from 1.5 to 3.0 and from 2.1 to 3.9 m/min in DP/DP and TRIP/TRIP steel weldments respectively. A top surface helium gas was used as a shielding gas at a flow rate of 20 l/min. Metallographic examinations and transverse tensile testing (DIN EN 895: 1995) were carried out to characterize the weldments. The formability of base metals and weldments were investigated by standard Erichsen test (DIN EN ISO 20482). It was found that the uniaxial plastic behavior of both DP600 and TRIP700 base metals was in agreement with Swift and modified Mecking–Kocks models respectively. In a perpendicular tensile test to the weld line, all specimens were fractured at the base metal however the strengths were somewhat higher than those of base metal. There was a significant reduction in formability caused by welding of both DP/DP and TRIP/TRIP steel weldments and the formability has been improved with the increase of the welding speed. Y1 - 2010 U6 - https://doi.org/http10.1016/j.jmatprotec.2010.08.003 SN - 0924-0136 VL - 210 IS - 15 SP - 2188 EP - 2196 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Reisgen, Uwe A1 - Schleser, Markus A1 - Mokrov, Oleg A1 - Ahmed, Essam T1 - Shielding gas influences on laser weldability of tailored blanks of advanced automotive steels JF - Applied surface science N2 - The effects of shielding gas types and flow rates on CO2 laser weldability of DP600/TRIP700 steel sheets were studied in this work. The evaluated shielding gases were helium (He), argon (Ar) and different mixtures of He and Ar. Weld penetration, tensile strength and formability (Erichsen test) of laser welds were found to be strongly dependent upon the shielding gas types. The ability of shielding gas in removing plasma plume and thus increasing weld penetration is believed to be closely related to ionization potential and atomic weight which determine the period of plasma formation and disappearance. It was found that the higher helium shielding gas flow rate, the deeper weld penetration and the lower weld width. Y1 - 2010 U6 - https://doi.org/10.1016/j.apsusc.2010.08.042 SN - 0169-4332 (E-Journal); 0169-4332 (Print) VL - 257 IS - 5 SP - 1401 EP - 1406 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Reisert, Steffen A1 - Henkel, Hartmut A1 - Schneider, Andreas A1 - Schäfer, Daniel A1 - Friedrich, Peter A1 - Berger, Jörg A1 - Schöning, Michael Josef T1 - Development of a handheld sensor system for the online measurement of hydrogen peroxide in aseptic filling systems JF - Physica Status Solidi (A). 207 (2010), H. 4 Y1 - 2010 SN - 1862-6300 N1 - Special Issue: Engineering of Functional Interfaces EnFI 2009 SP - 913 EP - 918 ER - TY - JOUR A1 - Recker, Elmar A1 - Bosschaerts, Walter A1 - Wagemakers, Rolf A1 - Hendrick, Patrick A1 - Funke, Harald A1 - Börner, Sebastian T1 - Experimental study of a round jet in cross-flow at low momentum ratio JF - 15th International Symposium on Applications of Laser Techniques to Fluid Mechanics Lisbon, Portugal, 05-08 July, 2010 - 1 Y1 - 2010 SP - 1 EP - 13 ER - TY - JOUR A1 - Probst, M. A1 - Behbahani, Mehdi A1 - Borrmann, E. A1 - Elgeti, S. A1 - Nicolai, M. A1 - Behr, M. T1 - Hemodynamic Modeling for Numerical Analysis and Design of Medical Devices Y1 - 2010 N1 - Posterpresentation ; NIC Symposium 2010 ; 24 - 25 February 2010 Jülich, Germany ER - TY - JOUR A1 - Pressler, Axel A1 - Esefeld, Katrin A1 - Scherr, Johannes A1 - Ali, Mohammad A1 - Hanssen, Henner A1 - Kotliar, Konstantin A1 - Lanzl, Ines A1 - Halle, Martin A1 - Kaemmerer, Harald A1 - Schmidt-Trucksäss, Arno A1 - Hager, Alfred T1 - Structural alterations of retinal arterioles in adults late after repair of aortic isthmic coarctation JF - The American Journal of Cardiology N2 - Patients after coarctation repair still have an increased risk of cardiovascular or cerebrovascular events. This has been explained by the persisting hypertension and alterations in the peripheral vessels. However, involvement of the central vessels such as the retinal arteries is virtually unknown. A total of 34 patients after coarctation repair (22 men and 12 women; 23 to 58 years old, age range 0 to 32 years at surgical repair) and 34 nonhypertensive controls underwent structural and functional retinal vessel analysis. Using structural analysis, the vessel diameters were measured. Using functional analysis, the endothelium-dependent vessel dilation in response to flicker light stimulation was assessed. In the patients after coarctation repair, the retinal arteriolar diameter was significantly reduced compared to that of the controls (median 182 μm, first to third quartile 171 to 197; vs 197 μm, first to third quartile 193 to 206; p <0.001). These findings were independent of the peripheral blood pressure and age at intervention. No differences were found for venules. The functional analysis findings were not different between the patients and controls (maximum dilation 3.5%, first to third quartile 2.1% to 4.5% vs 3.6%, first to third quartile 2.2% to 4.3%; p = 0.81), indicating preserved autoregulative mechanisms. In conclusion, the retinal artery diameter is reduced in patients after coarctation repair, independent of their current blood pressure level and age at intervention. As a structural marker of chronic vessel damage associated with past, current, or future hypertension, retinal arteriolar narrowing has been linked to stroke incidence. These results indicate an involvement of cerebral microcirculation in aortic coarctation, despite timely repair, and might contribute to explain the increased rate of cerebrovascular events in such patients. Y1 - 2010 U6 - https://doi.org/10.1016/j.amjcard.2009.10.070 SN - 0002-9149 VL - 105 IS - 5 SP - 740 EP - 744 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Pieper, Martin A1 - Klein, Peter T1 - Numerical solution of the heat equation with non-linear, time derivative-dependent source term JF - International Journal for Numerical Methods in Engineering N2 - The mathematical modeling of heat conduction with adsorption effects in coated metal structures yields the heat equation with piecewise smooth coefficients and a new kind of source term. This term is special, because it is non-linear and furthermore depends on a time derivative. In our approach we reformulated this as a new problem for the usual heat equation, without source term but with a new non-linear coefficient. We gave an existence and uniqueness proof for the weak solution of the reformulated problem. To obtain a numerical solution, we developed a semi-implicit and a fully implicit finite volume method. We compared these two methods theoretically as well as numerically. Finally, as practical application, we simulated the heat conduction in coated aluminum fibers with adsorption in the zeolite coating. Copyright © 2010 John Wiley & Sons, Ltd. Y1 - 2010 U6 - https://doi.org/10.1002/nme.2937 SN - 0029-5981 VL - 84 IS - 10 SP - 1205 EP - 1221 PB - Wiley CY - Chichester ER - TY - JOUR A1 - Pham, Phu Tinh A1 - Vu, Khoi Duc A1 - Tran, Thanh Ngoc A1 - Staat, Manfred T1 - A primal-dual algorithm for shakedown analysis of elastic-plastic bounded linearly kinematic hardening bodies Y1 - 2010 N1 - ECCM 2010, IV European Conference on Computational Mechanics, Paris, France, May 16-21, 2010. SP - 1 EP - 7 ER - TY - JOUR A1 - Paulßen, Elisabeth A1 - Schweighöfer, Philip V. A1 - Abram, Ulrich T1 - Reactions of [ReOX3(PPh3)2] Complexes (X = Cl, Br) with Phenylacetylene and the Structures of the Products JF - Zeitschrift für anorganische und allgemeine Chemie : ZAAC = Journal of inorganic and general chemistry N2 - Oxorhenium(V) complexes [ReOX3(PPh3)2] (X = Cl, Br) react with phenylacetylene under formation of complexes with ylide-type ligands. Compounds of the compositions [ReOCl3(PPh3){C(Ph)C(H)(PPh3)}] (1), [ReOBr3(OPPh3){C(Ph)C(H)(PPh3)}] (2), and [ReOBr3(OPPh3){C(H)C(Ph)(PPh3)}] (3) were isolated and characterized by X-ray diffraction. They contain a ligand, which was formed by a nucleophilic attack of released PPh3 at coordinated phenylacetylene. The structures of the products show that there is no preferable position for this attack. Cleavage of the Re–C bond in 3 and dimerization of the organic ligand resulted in the formation of the [{(PPh3)(H)CC(Ph)}2]2+ cation, which crystallized as its [(ReOBr4)(OReO3)]2– salt. Y1 - 2010 U6 - https://doi.org/10.1002/zaac.200900478 SN - 1521-3749 VL - 636 IS - 5 SP - 779 EP - 783 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Paulßen, Elisabeth A1 - Alberto, Roger A1 - Abram, Ulrich T1 - Synthesis, Characterization, and Structures of R3EOTcO3 Complexes (E = C, Si, Ge, Sn, Pb) and Related Compounds JF - Inorganic Chemistry N2 - AgTcO4 reacts with R3ECl compounds (E = C, Si, Ge, Sn, Pb; R = Me, iPr, tBu, Ph), tBu2SnCl2, or PhMgCl under formation of novel trioxotechnetium(VII) derivatives. The carbon and silicon derivatives readily undergo decomposition, which was proven by 99Tc NMR spectroscopy and the isolation of decomposition products such as [TcOCl3(THF)(OH2)]. Compounds [Ph3GeOTcO3], [(THF)Ph3SnOTcO3], [(O3TcO)SntBu2(OH)]2, and [(THF)4Mg(OTcO3)2] are more stable and were isolated in crystalline form and characterized by X-ray diffraction. Y1 - 2010 U6 - https://doi.org/10.1021/ic1001094 SN - 1520-510X VL - 49 IS - 7 SP - 3525 EP - 3530 PB - American Chemical Society CY - Washington ER - TY - JOUR A1 - Orzada, Stephan A1 - Maderwald, Stefan A1 - Poser, Benedikt Andreas A1 - Bitz, Andreas A1 - Quick, Harald H. A1 - Ladd, Mark E. T1 - RF excitation using time interleaved acquisition of modes (TIAMO) to address B1 inhomogeneity in high-field MRI JF - Magnetic Resonance in Medicine N2 - As the field strength and, therefore, the operational frequency in MRI is increased, the wavelength approaches the size of the human head/body, resulting in wave effects, which cause signal decreases and dropouts. Several multichannel approaches have been proposed to try to tackle these problems, including RF shimming, where each element in an array is driven by its own amplifier and modulated with a certain (constant) amplitude and phase relative to the other elements, and Transmit SENSE, where spatially tailored RF pulses are used. In this article, a relatively inexpensive and easy to use imaging scheme for 7 Tesla imaging is proposed to mitigate signal voids due to B1 field inhomogeneity. Two time-interleaved images are acquired using a different excitation mode for each. By forming virtual receive elements, both images are reconstructed together using GRAPPA to achieve a more homogeneous image, with only small SNR and SAR penalty in head and body imaging at 7 Tesla. Y1 - 2010 U6 - https://doi.org/10.1002/mrm.22527 SN - 1522-2594 VL - 64 IS - 2 SP - 327 EP - 333 PB - Wiley-Liss CY - New York ER - TY - JOUR A1 - Niemüller, Tim A1 - Ferrein, Alexander A1 - Lakemeyer, Gerhard T1 - A Lua-based Behavior Engine for Controlling the Humanoid Robot Nao JF - RoboCup 2009: Robot Soccer World Cup XIII Y1 - 2010 N1 - Lecture Notes in Computer Science ; 5949 SP - 240 EP - 251 ER - TY - JOUR A1 - Niemüller, Tim A1 - Ferrein, Alexander A1 - Beck, Daniel A1 - Lakemeyer, Gerhard T1 - Design Principles of the Component-Based Robot Software Framework Fawkes JF - Simulation, Modeling, and Programming for Autonomous Robots Y1 - 2010 N1 - Lecture Notes in Computer Science ; 6472 ; Second International Conference, SIMPAR 2010, Darmstadt, Germany, November 15-18, 2010. Proceedings SP - 300 EP - 311 ER - TY - JOUR A1 - Nguyen, N.-H. A1 - Raatschen, Hans-Jürgen A1 - Staat, Manfred T1 - A hyperelastic model of biological tissue materials in tubular organs Y1 - 2010 N1 - ECCM 2010, IV European Conference on Computational Mechanics, Paris, France, May 16-21, 2010. SP - 1 EP - 12 ER -