TY  - CHAP
A1  - Raman, Aravind Hariharan
A1  - Jung, Alexander
A1  - Horváth, András
A1  - Becker, Nadine
A1  - Staat, Manfred
ED  - Staat, Manfred
ED  - Erni, Daniel
T1  - Modification of a computer model of human stem cell-derived cardiomyocyte electrophysiology based on Patch-Clamp measurements
T2  - 3rd YRA MedTech Symposium 2019 : May 24 / 2019 / FH Aachen
N2  - Human induced pluripotent stem cells (hiPSCs) have shown to be promising in disease studies and drug screenings [1]. Cardiomyocytes derived from hiPSCs have been extensively investigated using patch-clamping and optical methods to compare their electromechanical behaviour relative to fully matured adult cells. Mathematical models can be used for translating findings on hiPSCCMs to adult cells [2] or to better understand the mechanisms of various ion channels when a drug is applied [3,4]. Paci et al. (2013) [3] developed the first model of hiPSC-CMs, which they later refined based on new data [3]. The model is based on iCells® (Fujifilm Cellular Dynamics, Inc. (FCDI), Madison WI, USA) but major differences among several cell lines and even within a single cell line have been found and motivate an approach for creating sample-specific models. We have developed an optimisation algorithm that parameterises the conductances (in S/F=Siemens/Farad) of the latest Paci et al. model (2018) [5] using current-voltage data obtained in individual patch-clamp experiments derived from an automated patch clamp system (Patchliner, Nanion Technologies GmbH, Munich).
Y1  - 2019
SN  - 978-3-940402-22-6
U6  - http://dx.doi.org/10.17185/duepublico/48750
SP  - 10
EP  - 11
PB  - Universität Duisburg-Essen
CY  - Duisburg
ER  - 
TY  - CHAP
A1  - Hunker, Jan
A1  - Jung, Alexander
A1  - Goßmann, Matthias
A1  - Linder, Peter
A1  - Staat, Manfred
ED  - Staat, Manfred
ED  - Erni, Daniel
T1  - Development of a tool to analyze the conduction speed in microelectrode array measurements of cardiac tissue
T2  - 3rd YRA MedTech Symposium 2019 : May 24 / 2019 / FH Aachen
N2  - The discovery of human induced pluripotent stem cells reprogrammed from somatic cells [1] and their ability to differentiate into cardiomyocytes (hiPSC-CMs) has provided a robust platform for drug screening [2]. Drug screenings are essential in the development of new components, particularly for evaluating the potential of drugs to induce life-threatening pro-arrhythmias. Between 1988 and 2009, 14 drugs have been removed from the market for this reason [3]. The microelectrode array (MEA) technique is a robust tool for drug screening as it detects the field potentials (FPs) for the entire cell culture. Furthermore, the propagation of the field potential can be examined on an electrode basis. To analyze MEA measurements in detail, we have developed an open-source tool.
Y1  - 2019
SN  - 978-3-940402-22-6
U6  - http://dx.doi.org/10.17185/duepublico/48750
SP  - 7
EP  - 8
PB  - Universität Duisburg-Essen
CY  - Duisburg
ER  -