TY  - JOUR
A1  - Aboulnaga, E. A.
A1  - Zou, H.
A1  - Selmer, Thorsten
A1  - Xian, M.
T1  - Development of a plasmid-based, tunable, tolC-derived expression system for application in Cupriavidus necator H16
JF  - Journal of Biotechnology
N2  - Cupriavidus necator H16 gains increasing attention in microbial research and biotechnological application due to its diverse metabolic features. Here we present a tightly controlled gene expression system for C. necator including the pBBR1-vector that contains hybrid promoters originating from C. necator native tolC-promoter in combination with a synthetic tetO-operator. The expression of the reporter gene from these plasmids relies on the addition of the exogenous inducer doxycycline (dc). The novel expression system offers a combination of advantageous features as; (i) high and dose-dependent recombinant protein production, (ii) tight control with a high dynamic range (On/Off ratio), which makes it applicable for harmful pathways or for toxic protein production, (iii) comparable cheap inducer (doxycycline, dc), (iv) effective at low inducer concentration, that makes it useful for large scale application, (v) rapid, diffusion controlled induction, and (vi) the inducer does not interfere within the cell metabolism. As applications of the expression system in C. necator H16, the growth ability on glycerol was enhanced by constitutively expressing the E. coli glpk gene-encoding for glycerol kinase. Likewise, we used the system to overcome the expression toxicity of mevalonate pathway in C. necator H16. With this system, the mevalonate-genes were successfully introduced in the host and the recombinant strains could produce about 200 mg/l mevalonate.
Y1  - 2018
U6  - http://dx.doi.org/10.1016/j.jbiotec.2018.03.007
SN  - 0168-1656
VL  - 274
SP  - 15
EP  - 27
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Aboulnaga, E. H.
A1  - Pinkenburg, O.
A1  - Schiffels, Johannes
A1  - El-Refai, A.
A1  - Buckel, W.
A1  - Selmer, Thorsten
T1  - Butyrate production in Escherichia coli: Exploitation of an oxygen tolerant bifurcating butyryl-CoA dehydrogenase/electron transferring flavoprotein complex from Clostridium difficile
JF  - Journal of bacteriology. June 14, 2013
Y1  - 2013
SN  - 1098-5530 (E-Journal) ; 0021-9193 (Print)
SP  - Epub ahead of print
ER  - 
TY  - JOUR
A1  - Abulnaga, El-Hussiny
A1  - Pinkenburg, Olaf
A1  - Schiffels, Johannes
A1  - E-Refai, Ahmed
A1  - Buckel, Wolfgang
A1  - Selmer, Thorsten
T1  - Effect of an Oxygen-Tolerant Bifurcating Butyryl Coenzyme A Dehydrogenase/Electron-Transferring Flavoprotein Complex from Clostridium difficile on Butyrate Production in Escherichia coli
JF  - Journal of bacteriology
Y1  - 2013
SN  - 1098-5530 [E-Journal]
SN  - 0021-9193 [Print]
VL  - 195
IS  - 16
SP  - 3704
EP  - 3713
ER  - 
TY  - JOUR
A1  - Adels, Klaudia
A1  - Elbers, Gereon
A1  - Diehl, Bernd
A1  - Monakhova, Yulia
T1  - Multicomponent analysis of dietary supplements containing glucosamine and chondroitin: comparative low- and high-field NMR spectroscopic study
JF  - Analytical Sciences
N2  - With the prevalence of glucosamine- and chondroitin-containing dietary supplements for people with osteoarthritis in the marketplace, it is important to have an accurate and reproducible analytical method for the quantitation of these compounds in finished products. NMR spectroscopic method based both on low- (80 MHz) and high- (500–600 MHz) field NMR instrumentation was established, compared and validated for the determination of chondroitin sulfate and glucosamine in dietary supplements. The proposed method was applied for analysis of 20 different dietary supplements. In the majority of cases, quantification results obtained on the low-field NMR spectrometer are similar to those obtained with high-field 500–600 MHz NMR devices. Validation results in terms of accuracy, precision, reproducibility, limit of detection and recovery demonstrated that the developed method is fit for purpose for the marketed products. The NMR method was extended to the analysis of methylsulfonylmethane, adulterant maltodextrin, acetate and inorganic ions. Low-field NMR can be a quicker and cheaper alternative to more expensive high-field NMR measurements for quality control of the investigated dietary supplements. High-field NMR instrumentation can be more favorable for samples with complex composition due to better resolution, simultaneously giving the possibility of analysis of inorganic species such as potassium and chloride.
KW  - Glucosamine
KW  - Chondroitin sulfate
KW  - Polysaccharides
KW  - Dietary supplements
KW  - High-field NMR
Y1  - 2023
U6  - http://dx.doi.org/10.1007/s44211-023-00433-2
SN  - 1348-2246 (Online)
SN  - 0910-6340 (Print)
N1  - Corresponding author: Yulia Monakhova
VL  - 2023
PB  - Springer Verlag
CY  - Cham
ER  - 
TY  - JOUR
A1  - Aggarwal, P.
A1  - Dhiman, S.
A1  - Kumar, G.
A1  - Scherer, Ulrich W.
A1  - Singla, M. L.
A1  - Srivastava, A.
T1  - Optical study of poly(ethyleneterephthalate) modified by different ionizing radiation dose
JF  - Indian Journal of Pure and Applied Physics
N2  - Thin films of poly(ethyleneterephthalate) [PET]were exposed to radiation dose ranging from 10 to 30 kGy by using gamma rays in the range 12.8-177.8 MGy using swift light ions of hydrogen. There was no effect of the radiation dose on the optical behaviour of PET as a result of exposure to radiation dose up to 30 kGy brought about by gamma rays but a significant decrease in the optical band gap values was observed when PET was exposed to swift light ions of hydrogen. The data obtained are discussed in terms of optical studies carried out on PET using swift heavy ions.
Y1  - 2012
SN  - 0019-5596
VL  - 50
IS  - 2
SP  - 129
EP  - 132
ER  - 
TY  - JOUR
A1  - Aggarwal, Prerna
A1  - Singh, Virendra
A1  - Singh, Arjun
A1  - Scherer, Ulrich W.
A1  - Singh, Tejvir
A1  - Singla, Madan L.
A1  - Srivastava, Alok
T1  - Physico-chemical transformations in swift heavy ion modified poly(ethyleneterephthalate)
JF  - Radiation Physics and Chemistry
Y1  - 2011
SN  - 0969-806X
N1  - In Press, Accepted Manuscript
SP  - 1
EP  - 28
PB  - Pergamon Press
CY  - Oxford
ER  - 
TY  - JOUR
A1  - Al-Kaidy, Huschyar
A1  - Duwe, Anna
A1  - Huster, Manuel
A1  - Muffler, Kai
A1  - Schlegel, Christin
A1  - Sieker, Tim
A1  - Stadtmüller, Ralf
A1  - Tippkötter, Nils
A1  - Ulber, Roland
T1  - Biotechnologie und Bioverfahrenstechnik – Vom ersten Ullmanns Artikel bis hin zu aktuellen Forschungsthemen
JF  - Chemie Ingenieur Technik
N2  - Biotechnologie und die mit ihr verbundenen technischen Prozesse prägen seit Jahrtausenden die Entwicklung der Menschheit. Ausgehend von empirischen Verfahren, insbesondere zur Herstellung von Lebensmitteln und täglichen Gebrauchsgütern, haben sich diese Disziplinen zu einem der innovativsten Zukunftsfelder entwickelt. Durch das immer detailliertere Verständnis zellulärer Vorgänge können mittlerweile Produktionsstämme gezielt optimiert werden. Im Zusammenspiel mit moderner Prozesstechnik können so eine Vielzahl von Bulk- und Feinchemikalien sowie Pharmazeutika effizient hergestellt werden. In diesem Artikel werden exemplarisch einige der aktuellen Trends vorgestellt.
Y1  - 2014
U6  - http://dx.doi.org/10.1002/cite.201400083
SN  - 0009-286X
VL  - 86
IS  - 12
SP  - 2215
EP  - 2225
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Al-Kaidy, Huschyar
A1  - Duwe, Anna
A1  - Huster, Manuel
A1  - Muffler, Kai
A1  - Schlegel, Christin
A1  - Tim, Sieker
A1  - Stadtmüller, Ralf
A1  - Tippkötter, Nils
A1  - Ulber, Roland
T1  - Biotechnology and bioprocess engineering – from the first ullmann's article to recent trends
JF  - ChemBioEng Reviews
N2  - For several thousand years, biotechnology and its associated technical processes have had a great impact on the development of mankind. Based on empirical methods, in particular for the production of foodstuffs and daily commodities, these disciplines have become one of the most innovative future issues. Due to the increasing detailed understanding of cellular processes, production strains can now be optimized. In combination with modern bioprocesses, a variety of bulk and fine chemicals as well as pharmaceuticals can be produced efficiently. In this article, some of the current trends in biotechnology are discussed.
Y1  - 2015
U6  - http://dx.doi.org/10.1002/cben.201500008
VL  - 2
IS  - 3
SP  - 175
EP  - 184
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Al-Kaidy, Huschyar
A1  - Kuthan, Kai
A1  - Hering, Thomas
A1  - Tippkötter, Nils
T1  - Aqueous droplets used as enzymatic microreactors and their electromagnetic actuation
JF  - Journal of Visualized Experiments
N2  - For the successful implementation of microfluidic reaction systems, such as PCR and electrophoresis, the movement of small liquid volumes is essential. In conventional lab-on-a-chip-platforms, solvents and samples are passed through defined microfluidic channels with complex flow control installations. The droplet actuation platform presented here is a promising alternative. With it, it is possible to move a liquid drop (microreactor) on a planar surface of a reaction platform (lab-in-a-drop). The actuation of microreactors on the hydrophobic surface of the platform is based on the use of magnetic forces acting on the outer shell of the liquid drops which is made of a thin layer of superhydrophobic magnetite particles. The hydrophobic surface of the platform is needed to avoid any contact between the liquid core and the surface to allow a smooth movement of the microreactor. On the platform, one or more microreactors with volumes of 10 µL can be positioned and moved simultaneously. The platform itself consists of a 3 x 3 matrix of electrical double coils which accommodate either neodymium or iron cores. The magnetic field gradients are automatically controlled. By variation of the magnetic field gradients, the microreactors' magnetic hydrophobic shell can be manipulated automatically to move the microreactor or open the shell reversibly. Reactions of substrates and corresponding enzymes can be initiated by merging the microreactors or bringing them into contact with surface immobilized catalysts.
Y1  - 2016
U6  - http://dx.doi.org/10.3791/54643
SN  - 1940-087X
IS  - Issue 126
ER  - 
TY  - JOUR
A1  - Al-Kaidy, Huschyar
A1  - Tippkötter, Nils
T1  - Superparamagnetic hydrophobic particles as shell material for digital microfluidic droplets and proof-of-principle reaction assessments with immobilized laccase
JF  - Engineering in Life Sciences
N2  - In the field of biotechnology and molecular biology, the use of small liquid volumes has significant advantages. In particular, screening and optimization runs with acceptable amounts of expensive and hardly available catalysts, reagents, or biomolecules are feasible with microfluidic technologies. The presented new microfluidic system is based on the inclusion of small liquid volumes by a protective shell of magnetizable microparticles. Hereby, discrete aqueous microreactor drops with volumes of 1–30 μL can be formed on a simple planar surface. A digital movement and manipulation of the microreactor is performed by overlapping magnetic forces. The magnetic forces are generated by an electrical coil matrix positioned below a glass plate. With the new platform technology, several discrete reaction compartments can be moved simultaneously on one surface. Due to the magnetic fields, the reactors can even be merged to initiate reactions by mixing or positioned above surface-immobilized catalysts and then opened by magnetic force. Comparative synthesis routes of the magnetizable shell particles and superhydrophobic glass slides including their performance and stability with the reaction platform are described. The influence of diffusive mass transport during the catalyzed reaction is discussed by evaluation finite element model of the microreactor. Furthermore, a first model dye reaction of the enzyme laccase has been established.
Y1  - 2016
U6  - http://dx.doi.org/10.1002/elsc.201400124
VL  - 16
IS  - 3
SP  - 222
EP  - 230
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Aletsee, Ludwig
A1  - Baumann, Marcus
T1  - A laboratory incubator equipped with facilities to automatically simulate natural irradiance
JF  - Boletim do Instituto Oceanográfico / Universidade de São Paulo
Y1  - 1991
SN  - 1982-436X; 0080-6331
VL  - Vol. 39
IS  - No. 2
SP  - 155
EP  - 159
ER  - 
TY  - JOUR
A1  - Balakrishnan, Karthikeyan
A1  - Andrei-Selmer, Luminita-Cornelia
A1  - Selmer, Thorsten
A1  - Bacher, Michael
A1  - Dodel, Richard
T1  - Comparison of Intravenous Immunoglobulins for Naturally Occurring Autoantibodies against Amyloid-β
JF  - Journal of Alzheimer's Disease
Y1  - 2010
SN  - 1387-2877
VL  - 20
IS  - 1
SP  - 135
EP  - 143
ER  - 
TY  - JOUR
A1  - Barbazán, Paula
A1  - Hagenbach, Adelheid
A1  - Paulßen, Elisabeth
A1  - Abram, Ulrich
A1  - Carballo, Rosa
A1  - Rodriguez-Hermida, Sabina
A1  - Vázquez-López, Ezequiel M.
T1  - Tricarbonyl Rhenium(I) and Technetium(I) Complexes with Hydrazones Derived from 4,5-Diazafluoren-9-one and 1,10-Phenanthroline-5,6-dione
JF  - European Journal of Inorganic Chemistry
N2  - Tricarbonylrhenium(I) and -technetium(I) halide (halide = Cl and Br) complexes of ligands derived from 4,5-diazafluoren-9-one (df) and 1,10-phenanthroline-5,6-dione (phen) derivatives of benzoic and 2-hydroxybenzoic acid hydrazides have been prepared. The complexes have been characterized by elemental analysis, MS, IR, 1H NMR and absorption and emission UV/Vis spectroscopic methods. The metal centres (ReI and TcI) are coordinated through the nitrogen imine atoms and establish five-membered chelate rings, whereas the hydrazone groups stand uncoordinated. The 1H NMR spectra suggest the same behaviour in solution on the basis of only marginal variations in the chemical shifts of the hydrazine protons.
Y1  - 2010
U6  - http://dx.doi.org/10.1002/ejic.201000522
SN  - 1099-0682
IS  - 29
SP  - 4622
EP  - 4630
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Baumann, Marcus
T1  - Algenblüten & Treibhauseffekt : Wechselwirkungen mit anthropogenen Aktivitäten
JF  - Wechselwirkung : Wissenschaft & vernetztes Denken
Y1  - 1996
SN  - 0172-1623
VL  - 18
IS  - 81
SP  - 6
EP  - 11
ER  - 
TY  - JOUR
A1  - Baumann, Marcus
A1  - Brandini, Frederico
A1  - Staubes, Regina
T1  - The influence of light and temperature on carbonspecific DMS release by cultures of Phaeocystis antarctica and three antarctic diatoms / Baumann, Marcus E.M. ; Brandini, Frederico ; Staubes, Regina
JF  - Marine Chemistry. 45 (1994), H. 1-2
Y1  - 1994
SN  - 0304-4203
SP  - 129
EP  - 136
ER  - 
TY  - JOUR
A1  - Baumann, Marcus
A1  - Goeyens, L.
A1  - Semeneh, M.
A1  - Elskens, M.
T1  - Phytoplankton nutrient utilisation and nutrient signature in the Southern Ocean / Goeyens, L. ; Semeneh, M. ; Baumann, M.E.M. ; Elskens, M. ; Shopova, D. ; Dehairs, F.
JF  - Journal of Marine Systems. 17 (1998), H. 1-4
Y1  - 1998
SN  - 0924-7963
SP  - 143
EP  - 157
ER  - 
TY  - JOUR
A1  - Baumann, Marcus
A1  - Goeyens, L.
A1  - Tréguer, P.
A1  - Baeyens, W.
T1  - The leading role of ammonium in the nitrogen uptake regime of Southern Ocean marginal ice zones / Goeyens, L. ; Tréguer, P. ; Baumann, M.E.M. ; Baeyens, W. ; Dehairs, F.
JF  - Journal of Marine Systems. 6 (1995), H. 4
Y1  - 1995
SN  - 0924-7963
SP  - 345
EP  - 361
ER  - 
TY  - JOUR
A1  - Baumann, Marcus
A1  - Gradinger, R.
T1  - Distribution of Phytoplankton Communities in Relation to the Large Scale Hydrographical Regime in the Fram Strait 1984 / Gradinger, R. ; Baumann, M.E.M.
JF  - Marine Biology. 111 (1991), H. 2
Y1  - 1991
SN  - 0025-3162
SP  - 311
EP  - 321
ER  - 
TY  - JOUR
A1  - Baumann, Marcus
A1  - Gronsfeld, A.
A1  - Schütz-Lembach, G.
T1  - Nachdenklichkeit über Nachhaltigkeit : wie uns ein neues Wort alte Probleme neu entdecken läßt
JF  - Wechselwirkung : Wissenschaft & vernetztes Denken
Y1  - 1997
SN  - 0172-1623
VL  - 19
IS  - 87
SP  - 6
EP  - 11
ER  - 
TY  - JOUR
A1  - Baumann, Marcus
A1  - Hirche, H.-J.
A1  - Kattner, G.
A1  - Gradinger, R.
T1  - Plankton distribution and the impact of copepod grazing on primary production in Fram Strait, Greenland Sea / Hirche, H.-J. ; Baumann, M.E.M. ; Kattner, G.; Gradinger, R.
JF  - Journal of Marine Systems. 2 (1991), H. 3-4
Y1  - 1991
SN  - 0924-7963
SP  - 477
EP  - 494
ER  - 
TY  - JOUR
A1  - Baumann, Marcus
A1  - Jahnke, J.
T1  - Die marine Planktonalge Phaeocystis globosa / Jahnke, J. ; Baumann, M.
JF  - Mikrokosmos. 75 (1986), H. 12
Y1  - 1986
SN  - 0026-3680
SP  - 357
EP  - 359
ER  - 
TY  - JOUR
A1  - Baumann, Marcus
A1  - Jahnke, J.
T1  - Chemical and Physical Effects on the Shape and Growth of the Diatom Biddulphia sinensis GREVILLE in Batch Cultures: A Contribution to Bioindication in Plankton Ecology / Jahnke, J. ; Baumann, M.
JF  - Hydrobiological Bulletin . 17 (1983), H. 1
Y1  - 1983
SN  - 0165-1404
SP  - 5
EP  - 20
ER  - 
TY  - JOUR
A1  - Baumann, Marcus
A1  - Jahnke, J.
T1  - Marine Planktonalgen der Arktis I. Die Haptophycee Phaeocystis pouchetii / Baumann, M. ; Jahnke, J.
JF  - Mikrokosmos. 75 (1986), H. 9
Y1  - 1986
SN  - 0026-3680
SP  - 262
EP  - 265
ER  - 
TY  - JOUR
A1  - Baumann, Marcus
A1  - Krämer, M.
T1  - Marine Planktonalgen der Arktis II. Diatomeen / Baumann, M. ; Krämer, M.
JF  - Mikrokosmos. 75 (1986), H. 10
Y1  - 1986
SN  - 0026-3680
SP  - 306
EP  - 310
ER  - 
TY  - JOUR
A1  - Baumann, Marcus
A1  - Lancelot, C.
A1  - Brandini, F.
A1  - Sakshaug, E.
T1  - The taxonomic identity of the cosmopolitan prymnesiophyte Phaeocystis, a morphological and ecophysiological approach / Baumann, M.E.M. ; Lancelot, C. ; Brandini, F. ; Sakshaug, E. ; John M.
JF  - Journal of Marine Systems. 5 (1994), H. 1
Y1  - 1994
SN  - 0924-7963
SP  - 5
EP  - 22
ER  - 
TY  - JOUR
A1  - Baumann, Marcus
A1  - Lara, R.J.
A1  - Hubberten, D.N.
A1  - Thomas, D.N.
T1  - Dissolved organic matter studies in enclosed systems: Application of hydophobic fractionation for the assessment of organic nitrogen dynamics / Lara, R.J. ; Hubberten, U. ; Thomas, D.N. ; Baumann, M.E.M. ; Kattner, G.
JF  - Journal of Marine Systems. 13 (1997), H. 1-4
Y1  - 1997
SN  - 0924-7963
SP  - 155
EP  - 161
ER  - 
TY  - JOUR
A1  - Baumann, Marcus
A1  - Peschel, G.
T1  - Aktuelle Entwicklungen zur Luftreinhaltung in der Stadt Aachen
JF  - Wechselwirkung : Wissenschaft & vernetztes Denken
Y1  - 1998
SN  - 0172-1623
VL  - 20
IS  - 91
SP  - 22
EP  - 29
ER  - 
TY  - JOUR
A1  - Baumann, Marcus
A1  - Rousseau, V.
A1  - Vaulot, D.
A1  - Casotti, R.
T1  - The life cycle of Phaeocystis (Prymnesiophycae): evidence and hypotheses / Rousseau, V. ; Vaulot, D. ; Casotti, R. ; Cariou, V. ; Lenz, J. ; Gunkel, J. ; Baumann, M.
JF  - Journal of Marine Systems. 5 (1994), H. 1
Y1  - 1994
SN  - 0924-7963
SP  - 23
EP  - 39
ER  - 
TY  - JOUR
A1  - Baumann, Marcus
A1  - Semeneh, M.
A1  - Dehairs, F.
A1  - Elskens, M.
T1  - Nitrogen uptake regime and phytoplankton community structure in the Southern Ocean / Semeneh, M. ; Dehairs, F. ; Elskens, M. ; Baumann, M.E.M. ; Kopczynska, E.E. ; Lancelot, C. ; Goeyens, L.
JF  - Journal of Marine Systems. 17 (1998), H. 1-4
Y1  - 1998
SN  - 0924-7963
SP  - 159
EP  - 177
ER  - 
TY  - JOUR
A1  - Baumann, Marcus
A1  - Thomas, D.
A1  - Gleitz, M.
T1  - Efficiency of carbon assimilation and photoacclimation in a small unicellular Chaetoceros species from the Weddel Sea (Antarctica): Influence of temperature and irridiance / Thomas, D. ; Baumann, M.E.M. ; Gleitz, M.
JF  - Journal of Experimental Marine Biology and Ecology. 157 (1992), H. 2
Y1  - 1992
SN  - 0022-0981
SP  - 195
EP  - 209
ER  - 
TY  - JOUR
A1  - Baumann, Marcus
A1  - Tillmann, U.
A1  - Aletsee, L.
T1  - Distribution of Carbon Among Photosynthetic End Products in the Bloom-Forming Arctic Diatom Thalassiosira antarctica COMBER / Tillmann, U. ; Baumann, M.E.M. ; Aletsee, L.
JF  - Polar Biology. 10 (1989), H. 3
Y1  - 1989
SN  - 0722-4060
SP  - 231
EP  - 238
ER  - 
TY  - JOUR
A1  - Becht, Alexander
A1  - Schollmayer, Curd
A1  - Monakhova, Yulia
A1  - Holzgrabe, Ulrike
T1  - Tracing the origin of paracetamol tablets by near-infrared, mid-infrared, and nuclear magnetic resonance spectroscopy using principal component analysis and linear discriminant analysis
JF  - Analytical and Bioanalytical Chemistry
N2  - Most drugs are no longer produced in their own countries by the pharmaceutical companies, but by contract manufacturers or at manufacturing sites in countries that can produce more cheaply. This not only makes it difficult to trace them back but also leaves room for criminal organizations to fake them unnoticed. For these reasons, it is becoming increasingly difficult to determine the exact origin of drugs. The goal of this work was to investigate how exactly this is possible by using different spectroscopic methods like nuclear magnetic resonance and near- and mid-infrared spectroscopy in combination with multivariate data analysis. As an example, 56 out of 64 different paracetamol preparations, collected from 19 countries around the world, were chosen to investigate whether it is possible to determine the pharmaceutical company, manufacturing site, or country of origin. By means of suitable pre-processing of the spectra and the different information contained in each method, principal component analysis was able to evaluate manufacturing relationships between individual companies and to differentiate between production sites or formulations. Linear discriminant analysis showed different results depending on the spectral method and purpose. For all spectroscopic methods, it was found that the classification of the preparations to their manufacturer achieves better results than the classification to their pharmaceutical company. The best results were obtained with nuclear magnetic resonance and near-infrared data, with 94.6%/99.6% and 98.7/100% of the spectra of the preparations correctly assigned to their pharmaceutical company or manufacturer.
KW  - IR
KW  - Manufacturer
KW  - Linear discriminant analysis
KW  - Principal component analysis
Y1  - 2021
U6  - http://dx.doi.org/10.1007/s00216-021-03249-z
SN  - 1618-2650
VL  - 413
SP  - 3107
EP  - 3118
PB  - Springer Nature
ER  - 
TY  - JOUR
A1  - Bergs, Michel
A1  - Monakhova, Yulia
A1  - Diehl, Bernd W.
A1  - Konow, Christopher
A1  - Völkering, Georg
A1  - Pude, Ralf
A1  - Schulze, Margit
T1  - Lignins isolated via catalyst-free organosolv pulping from Miscanthus x giganteus, M. sinensis, M. robustus and M. nagara: a comparative study
JF  - Molecules
N2  - As a low-input crop, Miscanthus offers numerous advantages that, in addition to agricultural applications, permits its exploitation for energy, fuel, and material production. Depending on the Miscanthus genotype, season, and harvest time as well as plant component (leaf versus stem), correlations between structure and properties of the corresponding isolated lignins differ. Here, a comparative study is presented between lignins isolated from M. x giganteus, M. sinensis, M. robustus and M. nagara using a catalyst-free organosolv pulping process. The lignins from different plant constituents are also compared regarding their similarities and differences regarding monolignol ratio and important linkages. Results showed that the plant genotype has the weakest influence on monolignol content and interunit linkages. In contrast, structural differences are more significant among lignins of different harvest time and/or season. Analyses were performed using fast and simple methods such as nuclear magnetic resonance (NMR) spectroscopy. Data was assigned to four different linkages (A: β-O-4 linkage, B: phenylcoumaran, C: resinol, D: β-unsaturated ester). In conclusion, A content is particularly high in leaf-derived lignins at just under 70% and significantly lower in stem and mixture lignins at around 60% and almost 65%. The second most common linkage pattern is D in all isolated lignins, the proportion of which is also strongly dependent on the crop portion. Both stem and mixture lignins, have a relatively high share of approximately 20% or more (maximum is M. sinensis Sin2 with over 30%). In the leaf-derived lignins, the proportions are significantly lower on average. Stem samples should be chosen if the highest possible lignin content is desired, specifically from the M. x giganteus genotype, which revealed lignin contents up to 27%. Due to the better frost resistance and higher stem stability, M. nagara offers some advantages compared to M. x giganteus. Miscanthus crops are shown to be very attractive lignocellulose feedstock (LCF) for second generation biorefineries and lignin generation in Europe.
Y1  - 2021
U6  - http://dx.doi.org/10.3390/molecules26040842
SN  - 1420-3049
N1  - Special Issue Lignin – A Natural Resource with Huge Potential https://www.mdpi.com/journal/molecules/special_issues/lignin_natural
VL  - 26
IS  - 4
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Berndt, Heinz
T1  - Synthese der Sequenz 71—86 des Humanproinsulins, III : Synthese über die Fragmente 71—78 und 79—86
JF  - Hoppe-Seyler's Zeitschrift für physiologische Chemie
Y1  - 1979
U6  - http://dx.doi.org/10.1515/bchm2.1979.360.1.765
SN  - 1437-4315
SN  - 0018-4888
VL  - 360
IS  - 1
SP  - 765
EP  - 772
ER  - 
TY  - JOUR
A1  - Berndt, Heinz
T1  - Synthese der Sequenz 71—86 des Humanproinsulins, I : Synthese der Sequenz 71—86 als monomeres cyclisches Biscystinpeptidderivat und als Tetra-S-tritylderivat
JF  - Hoppe-Seyler's Zeitschrift für physiologische Chemie
Y1  - 1979
U6  - http://dx.doi.org/10.1515/bchm2.1979.360.1.747
SN  - 1437-4315
SN  - 0018-4888
VL  - 360
IS  - 1
SP  - 747
EP  - 760
ER  - 
TY  - JOUR
A1  - Berndt, Heinz
T1  - Zur Reaktion von Iminodithiocarbonaten mit Carbonsäuren. I : Synthese des Modellpeptid-Derivates Z-(L)-Ala-(L/D)-Phe-(L)-Val-OMe
JF  - Tetrahedron letters
Y1  - 1980
U6  - http://dx.doi.org/10.1016/S0040-4039(00)78663-1
SN  - 0040-4039
VL  - 21
IS  - 34
SP  - 3265
EP  - 3268
ER  - 
TY  - JOUR
A1  - Berndt, Heinz
A1  - Gattner, Hans-Gregor
A1  - Zahn, Helmut
T1  - Semisynthetisches Des-A1-glycin-Schafinsulin
JF  - Biological Chemistry
Y1  - 1975
U6  - http://dx.doi.org/10.1515/bchm2.1975.356.2.1455
SN  - 1437-4315
VL  - 356
IS  - 2
SP  - 1469
EP  - 1472
PB  - De Gruyter
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Berndt, Heinz
A1  - Klostermeyer, Henning
A1  - Zahn, Helmut
T1  - Zur Synthese monomerer cyclischer Cystinpeptidderivate, I : Synthese der Sequenz A 6–9 des Schafinsulins als Cyclocystinylderivat
JF  - Justus Liebigs Annalen der Chemie
N2  - Es wird die Synthese der Sequenz A 6–9 des Schafinsulins in der geschützten Form Boc-Cys-Cys-Ala-Gly-OBuᵗ (5) sowie das Verhalten dieses monomeren cyclischen Cystinpeptidderivates gegenüber den in der Peptidchemie gebräuchlichen Reagenzien Bortrifluorid/Eisessig, Triäthylamin und Hydrazinhydrat beschrieben.
Y1  - 1972
U6  - http://dx.doi.org/10.1002/jlac.19727590109
SN  - 1099-0690
VL  - 759
IS  - 1
SP  - 114
EP  - 120
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Berndt, Heinz
A1  - Krüger, Götz
T1  - Resolution of enantiomeric amino acid derivatives by high-performance liquid chromatography on chiral stationary phases
JF  - Journal of chromatography A
Y1  - 1985
U6  - http://dx.doi.org/10.1016/S0021-9673(01)92461-6
SN  - 0021-9673
VL  - 1985
IS  - 348
SP  - 275
EP  - 279
ER  - 
TY  - JOUR
A1  - Berndt, Heinz
A1  - Zahn, Helmut
T1  - Peptide, 99 : Monomere cyclische Cystinpeptidderivate, III ; Synthese der Schafinsulin-A-Kettensequenzen A2–21 und A1–21 als monomere cyclische Dicystinpeptidderivate
JF  - Justus Liebigs Annalen der Chemie
N2  - Die Synthese der Sequenzen A2—21 (13) und A1—21 (15) der Schafinsulin-A-Kette als monomere cyclische Dicystinpeptidderivate wird beschrieben. Die intrachenaren Cystinbrücken A6—7 und A 11 —20 vermitteln die Löslichkeit dieser Derivate in Dimethylformamid und ermöglichen erstmalig die Reindarstellung vollgeschützter Insulin-A-Kettenderivate. Die während der Synthese eingesetzten Schutzgruppen lassen sich mittels Trifluoressigsäure und 2-Mercaptoäthanol quantitativ entfernen.
Y1  - 1975
U6  - http://dx.doi.org/10.1002/jlac.197519750908
SN  - 1099-0690
VL  - 1975
IS  - 9
SP  - 1601
EP  - 1612
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Biselli, Manfred
A1  - Born, C.
A1  - Thömmes, J.
A1  - Wandrey, C.
T1  - An approach to integrated antibody production: Coupling of fluidized bed cultivation and  fluidized bed adsorption / Born, C. ; Thömmes, J. ; Biselli, M. ; Wandrey, C. ; Kula, M.-R.
JF  - Bioprocess Engineering. 15 (1996), H. 1
Y1  - 1996
SN  - 1615-7591
SP  - 21
EP  - 29
ER  - 
TY  - JOUR
A1  - Biselli, Manfred
A1  - Born, C.
A1  - Wandrey, C.
T1  - Oxygen transfer from the gasphase to the immobilized cells in membrane aerated  fluidized beds / Born, C. ; Biselli, M. ; Wandrey, C.
JF  - Animal cell technology : basic & applied aspects : proceedings of the Eighth Annual Meeting of the Japanese Association for Animal Cell Technology, Iizuka, Fukuoka, Japan, November 6-10, 1995 / edited by K. Funatsu, Y. Shirai, and T. Matsushita
Y1  - 1995
SN  - 0-7923-4486-3
N1  - Japanese Association for Animal Cell Technology. ; Meeting ; <8 ; 1995 : ; Iizuka-shi, Japan>
SP  - 83
EP  - 87
PB  - Kluwer Acad. Press
CY  - Boston
ER  - 
TY  - JOUR
A1  - Biselli, Manfred
A1  - Born, C.
A1  - Wandrey, C.
T1  - Production of monoclonal antibodies in a pilot scale fluidized bed bioreactor / Born, C.; Biselli, M.; Wandrey, C.
JF  - Animal cell technology : developments towards the 21st century / edited by E. C. Beuvery
Y1  - 1995
SN  - 0-7923-3736-0
N1  - Meeting "Animal Cell Technology, Developments towards the 21st Century" ; <1994.09.12-16 : ; Veldhoven>
SP  - 515
EP  - 519
PB  - Kluwer Acad. Press
CY  - Dordrecht
ER  - 
TY  - JOUR
A1  - Biselli, Manfred
A1  - Bäcker, Matthias
A1  - Poghossian, Arshak
A1  - Schöning, Michael Josef
A1  - Schnitzler, Thomas
A1  - Zang, Werner
A1  - Wagner, P.
T1  - Entwicklung eines modularen festkörperbasierten Sensorsystems für die Überwachung von Zellkulturfermenationen
JF  - Sensoren und Messsysteme 2010 [Elektronische Ressource] : Vorträge der 15. ITG/GMA-Fachtagung vom 18. bis 19. Mai 2010 in Nürnberg / Informationstechnische Gesellschaft im VDE (ITG); VDI/VDE-Gesellschaft Mess- und Automatisierungstechnik (GMA)
Y1  - 2010
SN  - 978-3-8007-3260-9
N1  - Fachtagung Sensoren und Messsysteme 15, 2010, Nürnberg ; Gesellschaft Mess- und Automatisierungstechnik
SP  - 688
EP  - 691
PB  - VDE Verlag
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Biselli, Manfred
A1  - Hambach, B.
A1  - Runstadler, P. W.
A1  - Wandrey, Christian
T1  - Development of a reactor-integrated aeration system for cultivation of animal cells in fluidized beds / Hambach, B. ; Biselli, M. ; Runstadler, P.W. ; Wandrey, C.
JF  - Animal cell technology : developments, processes, and products ; ESACT, European Society for Animal Cell Technology, the 11th meeting / Ed. R. E. Spier
Y1  - 1992
SN  - 0750604212
SP  - 381
EP  - 385
PB  - Butterworth-Heinemann
CY  - Oxford
ER  - 
TY  - JOUR
A1  - Biselli, Manfred
A1  - Hilbert, U.
A1  - Jelinek, N.
A1  - Schmidt, S.
T1  - Bioprocess development for the cultivation of human T-lymphocytes / Hilbert, U. ; Jelinek, N.  Schmidt, S. ; Biselli, M.
JF  - Engineering in Life Sciences. 1 (2001)
Y1  - 2001
SN  - 1618-0240
SP  - 20
EP  - 23
ER  - 
TY  - JOUR
A1  - Biselli, Manfred
A1  - Hilbert, U.
A1  - Noll, T.
T1  - Cultivation of Human HCMV Specific Lymphocytes – An Example for  Adoptive Immunotherapy / Hilbert, U. ; Biselli, M. ; Noll, T.
JF  - Animal cell technology : from target to market ; Tylösand, Sweden, June 10 - 14, 2001 / ed. by E. Lindner-Olsson ...
Y1  - 2001
SN  - 1-4020-0264-5
N1  - Proceedings of the ... ESACT meeting ; 17
SP  - 558
EP  - 561
PB  - Kluwer
CY  - Dordrecht
ER  - 
TY  - JOUR
A1  - Biselli, Manfred
A1  - Hilbert, Ulrike
A1  - Jelinek, Nanni
A1  - Schmidt, Sebastian
T1  - Biotechnologische Aspekte der Kultivierung humaner T-Lymphozyten / Hilbert, Ulrike ; Jelinek, Nanni ; Schmidt, Sebastian ; Biselli, Manfred
JF  - Chemie Ingenieur Technik. 72 (2000), H. 7
Y1  - 2000
SN  - 1522-2640
SP  - 746
EP  - 750
ER  - 
TY  - JOUR
A1  - Biselli, Manfred
A1  - Jelinek, N.
A1  - Schmidt, S.
A1  - Hilbert, U.
T1  - Neue Bioreaktoren zur Kultivierung hämatopoetischer Zellen / Jelinek, N. ; Schmidt, S. ; Hilbert, U. ; Thoma, S. ; Biselli, M. ; Wandrey, C.
JF  - Chemie Ingenieur Technik. 73 (2001), H. 7
Y1  - 2001
SN  - 0009-286X
SP  - 894
EP  - 898
ER  - 
TY  - JOUR
A1  - Biselli, Manfred
A1  - Jelinek, N.
A1  - Schmidt, S.
A1  - Hilbert, U.
T1  - Novel bioreactors for the ex vivo cultivation of hematopoietic cells / Jelinek, N. ; Schmidt, S. ; Hilbert, U. ; Thoma, S. ; Biselli, M. ; Wandrey, C.
JF  - Engineering in Life Sciences. 2 (2002), H. 1
Y1  - 2002
SN  - 1618-0240
SP  - 15
EP  - 18
ER  -