TY  - JOUR
A1  - Bandodkar, Amay J.
A1  - Molinnus, Denise
A1  - Mirza, Omar
A1  - Guinovart, Tomas
A1  - Windmiller, Joshua R.
A1  - Valdes-Ramirez, Gabriela
A1  - Andrade, Francisco J.
A1  - Schöning, Michael Josef
A1  - Wang, Joseph
T1  - Epidermal tattoo potentiometric sodium sensors with wireless signal transduction for continuous non-invasive sweat monitoring
JF  - Biosensors and bioelectronics
N2  - This article describes the fabrication, characterization and application of an epidermal temporary-transfer tattoo-based potentiometric sensor, coupled with a miniaturized wearable wireless transceiver, for real-time monitoring of sodium in the human perspiration. Sodium excreted during perspiration is an excellent marker for electrolyte imbalance and provides valuable information regarding an individual's physical and mental wellbeing. The realization of the new skin-worn non-invasive tattoo-like sensing device has been realized by amalgamating several state-of-the-art thick film, laser printing, solid-state potentiometry, fluidics and wireless technologies. The resulting tattoo-based potentiometric sodium sensor displays a rapid near-Nernstian response with negligible carryover effects, and good resiliency against various mechanical deformations experienced by the human epidermis. On-body testing of the tattoo sensor coupled to a wireless transceiver during exercise activity demonstrated its ability to continuously monitor sweat sodium dynamics. The real-time sweat sodium concentration was transmitted wirelessly via a body-worn transceiver from the sodium tattoo sensor to a notebook while the subjects perspired on a stationary cycle. The favorable analytical performance along with the wearable nature of the wireless transceiver makes the new epidermal potentiometric sensing system attractive for continuous monitoring the sodium dynamics in human perspiration during diverse activities relevant to the healthcare, fitness, military, healthcare and skin-care domains.
Y1  - 2014
U6  - https://doi.org/10.1016/j.bios.2013.11.039
SN  - 1873-4235 (E-Journal); 0956-5663 (Print)
VL  - 54
SP  - 603
EP  - 609
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Wiegand, Sandra
A1  - Dietrich, Sascha
A1  - Hertel, Robert
A1  - Bongaerts, Johannes
A1  - Evers, Stefan
A1  - Volland, Sonja
A1  - Daniel, Rolf
A1  - Liesegang, Heiko
T1  - RNA-Seq of Bacillus licheniformis: active regulatory RNA features expressed within a productive fermentation
JF  - BMC genomics
Y1  - 2013
SN  - 1471-2164
VL  - Vol. 14
SP  - 667
PB  - BioMed Central
CY  - London
ER  - 
TY  - JOUR
A1  - Rachinger, Michael
A1  - Bauch, Melanie
A1  - Strittmatter, Axel
A1  - Bongaerts, Johannes
A1  - Evers, Stefan
A1  - Maurer, Karl-Heinz
A1  - Daniel, Rolf
A1  - Liebl, Wolfgang
A1  - Liesegang, Heiko
A1  - Ehrenreich, Armin
T1  - Size unlimited markerless deletions by a transconjugative plasmid-system in Bacillus licheniformis
JF  - Journal of biotechnology
Y1  - 2013
SN  - 1873-4863 (E-Journal); 0168-1656 (Print)
VL  - Vol. 164
IS  - Iss. 4
SP  - 365
EP  - 369
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Voigt, Birgit
A1  - Schroeter, Rebecca
A1  - Jürgen, Britta
A1  - Albrecht, Dirk
A1  - Evers, Stefan
A1  - Bongaerts, Johannes
A1  - Maurer, Karl-Heinz
A1  - Schweder, Thomas
A1  - Hecker, Michael
T1  - The response of Bacillus licheniformis to heat and ethanol stress and the role of the SigB regulon
JF  - Proteomics
Y1  - 2013
SN  - 1615-9861 (E-Journal); 1615-9853 (Print)
VL  - Vol. 13
IS  - Iss. 14
SP  - 2140
EP  - 2146
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Wilming, Anja
A1  - Begemann, Jens
A1  - Kuhne, Stefan
A1  - Regestein, Lars
A1  - Bongaerts, Johannes
A1  - Evers, Stefan
A1  - Maurer, Karl-Heinz
A1  - Büchs, Jochen
T1  - Metabolic studies of γ-polyglutamic acid production in Bacillus licheniformis by small-scale continuous cultivations
JF  - Biochemical engineering journal
Y1  - 2013
SN  - 1873-295X (E-Journal); 1369-703X (Print)
VL  - Vol. 73
SP  - 29
EP  - 37
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Scheele, Sandra
A1  - Oertel, Dan
A1  - Bongaerts, Johannes
A1  - Evers, Stefan
A1  - Hellmuth, Hendrik
A1  - Maurer, Karl-Heinz
A1  - Bott, Michael
A1  - Freudl, Roland
T1  - Secretory production of an FAD cofactor-containing cytosolic enzyme (sorbitol–xylitol oxidase from Streptomyces coelicolor) using the twin-arginine translocation (Tat) pathway of Corynebacterium glutamicum
JF  - Microbial biotechnology
Y1  - 2013
SN  - 1751-7915
SP  - 202
EP  - 206
PB  - Wiley-Blackwell
CY  - Oxford
ER  - 
TY  - JOUR
A1  - Borgmeier, Claudia
A1  - Bongaerts, Johannes
A1  - Meinhardt, Friedhelm
T1  - Genetic analysis of the Bacillus licheniformis degSU operon and the impact of regulatory mutations on protease production
JF  - Journal of biotechnology
N2  - Disruption experiments targeted at the Bacillus licheniformis degSU operon and GFP-reporter analysis provided evidence for promoter activity immediately upstream of degU. pMutin mediated concomitant introduction of the degU32 allele – known to cause hypersecretion in Bacillus subtilis – resulted in a marked increase in protease activity. Application of 5-fluorouracil based counterselection through establishment of a phosphoribosyltransferase deficient Δupp strain eventually facilitated the marker-free introduction of degU32 leading to further protease enhancement achieving levels as for hypersecreting wild strains in which degU was overexpressed. Surprisingly, deletion of rapG – known to interfere with DegU DNA-binding in B. subtilis – did not enhance protease production neither in the wild type nor in the degU32 strain. The combination of degU32 and Δupp counterselection in the type strain is not only equally effective as in hypersecreting wild strains with respect to protease production but furthermore facilitates genetic strain improvement aiming at biological containment and effectiveness of biotechnological processes.
KW  - Marker-free mutagenesis
KW  - Extracellular enzymes
KW  - Uracil-phosphoribosyltransferase
KW  - Hypersecretion
Y1  - 2012
U6  - https://doi.org/10.1016/j.jbiotec.2012.02.011
SN  - 1873-4863 (E-Journal); 0168-1656 (Print)
VL  - 159
IS  - 1-2
SP  - 12
EP  - 20
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Bongaerts, Johannes
A1  - Esser, Simon
A1  - Lorbach, Volker
A1  - Al-Momani, Lóay
A1  - Müller, Michael A.
A1  - Franke, Dirk
A1  - Grondal, Christoph
A1  - Kurutsch, Anja
A1  - Bujnicki, Robert
A1  - Takors, Ralf
A1  - Raeven, Leon
A1  - Wubbolts, Marcel
A1  - Bovenberg, Roel
A1  - Nieger, Martin
A1  - Schürmann, Melanie
A1  - Trachtmann, Natalie
A1  - Kozak, Stefan
A1  - Sprenger, Georg A.
A1  - Müller, Michael
T1  - Diversity-oriented production of metabolites derived from chorismate and their use in organic synthesis
JF  - Angewandte Chemie International Edition
Y1  - 2011
SN  - 1521-3773 (E-Journal); 0570-0833 (Print); 1433-7851 (Print)
VL  - Vol. 50
IS  - Iss. 34
SP  - 7781
EP  - 7786
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Deppe, Veronika Maria
A1  - Klatte, Stephanie
A1  - Bongaerts, Johannes
A1  - Maurer, Karl-Heinz
A1  - O'Connell, Timothy
A1  - Meinhardt, Friedhelm
T1  - Genetic control of Amadori product degradation in Bacillus subtilis via regulation of frlBONMD expression by FrlR
JF  - Applied and environmental microbiology
Y1  - 2011
SN  - 1098-5336 (E-Journal); 0003-6919 (Print); 0099-2240 (Print)
VL  - Vol. 77
IS  - No. 9
SP  - 2839
EP  - 2846
PB  - American Society of Mechanical Engineers (ASME)
CY  - New York
ER  - 
TY  - JOUR
A1  - Deppe, Veronika Maria
A1  - Bongaerts, Johannes
A1  - O'Connell, Timothy
A1  - Maurer, Karl-Heinz
A1  - Meinhardt, Friedhelm
T1  - Enzymatic deglycation of Amadori products in bacteria
JF  - Applied microbiology and biotechnology
Y1  - 2011
SN  - 1432-0614 (E-Journal); 0171-1741 (Print); 0175-7598 (Print); 0340-2118 (Print)
VL  - Vol. 90
IS  - Iss. 2
SP  - 399
EP  - 406
PB  - Springer
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Degering, Christian
A1  - Eggert, Thorsten
A1  - Puls, Michael
A1  - Bongaerts, Johannes
A1  - Evers, Stefan
A1  - Maurer, Karl-Heinz
A1  - Jaeger, Karl-Erich
T1  - Optimization of protease secretion in Bacillus subtilis and Bacillus licheniformis by screening of homologous and herologous signal peptides
JF  - Applied and environmental microbiology
N2  - Bacillus subtilis and Bacillus licheniformis are widely used for the large-scale industrial production of proteins. These strains can efficiently secrete proteins into the culture medium using the general secretion (Sec) pathway. A characteristic feature of all secreted proteins is their N-terminal signal peptides, which are recognized by the secretion machinery. Here, we have studied the production of an industrially important secreted protease, namely, subtilisin BPN′ from Bacillus amyloliquefaciens. One hundred seventy-three signal peptides originating from B. subtilis and 220 signal peptides from the B. licheniformis type strain were fused to this secretion target and expressed in B. subtilis, and the resulting library was analyzed by high-throughput screening for extracellular proteolytic activity. We have identified a number of signal peptides originating from both organisms which produced significantly increased yield of the secreted protease. Interestingly, we observed that levels of extracellular protease were improved not only in B. subtilis, which was used as the screening host, but also in two different B. licheniformis strains. To date, it is impossible to predict which signal peptide will result in better secretion and thus an improved yield of a given extracellular target protein. Our data show that screening a library consisting of homologous and heterologous signal peptides fused to a target protein can identify more-effective signal peptides, resulting in improved protein export not only in the original screening host but also in different production strains.
Y1  - 2010
U6  - https://doi.org/10.1128/AEM.01146-10
SN  - 1098-5336 (E-Journal); 0003-6919 (Print); 0099-2240 (Print)
VL  - 76
IS  - 19
SP  - 6370
EP  - 6378
PB  - American Society for Microbiology
CY  - Washington, DC
ER  - 
TY  - JOUR
A1  - Scheele, S.
A1  - Bongaerts, Johannes
A1  - Maurer, K.-H.
A1  - Freudl, R.
T1  - Sekretion einer Kofaktor-haltigen Oxidase durch Corynebacterium glutamicum
JF  - Chemie - Ingenieur - Technik (CIT)
Y1  - 2009
SN  - 1522-2640 (E-Journal); 0009-286X (Print)
VL  - Vol. 81
IS  - Iss. 8
SP  - 1309
ER  - 
TY  - JOUR
A1  - Polen, T.
A1  - Krämer, Marco
A1  - Bongaerts, Johannes
A1  - Wubbolts, Marcel
A1  - Wendisch, V. F.
T1  - The global gene expression response of Escherichia coli to L-phenylalanine
JF  - Journal of biotechnology
Y1  - 2005
SN  - 1873-4863 (E-Journal); 0168-1656 (Print)
VL  - Vol. 115
IS  - Iss. 3
SP  - 221
EP  - 237
ER  - 
TY  - JOUR
A1  - Krämer, Marco
A1  - Bongaerts, Johannes
A1  - Bovenberg, Roel
A1  - Kremer, Susanne
A1  - Müller, Ulrike
A1  - Orf, Sonja
A1  - Wubbolts, Marcel
A1  - Raeven, Leon
T1  - Metabolic engineering for microbial production of shikimic acid
JF  - Metabolic engineering
Y1  - 2003
SN  - 1096-7184 (E-Journal); 1096-7176 (Print)
VL  - Vol. 5
IS  - Iss. 4
SP  - 277
EP  - 283
ER  - 
TY  - JOUR
A1  - Gerigk, M.
A1  - Maaß, D.
A1  - Kreutzer, A.
A1  - Sprenger, G.
A1  - Bongaerts, Johannes
A1  - Wubbolts, Marcel
A1  - Takors, Ralf
T1  - Enhanced pilot-scale fed-batch L-phenylalanine production with recombinant Escherichia coli by fully integrated reactive extraction
JF  - Bioprocess and biosystems engineering
Y1  - 2002
SN  - 1432-0797 (E-Journal); 1615-7605 (E-Journal); 0178-515X (Print); 1615-7591 (Print)
VL  - Vol. 25
IS  - Iss. 1
SP  - 43
EP  - 52
ER  - 
TY  - JOUR
A1  - Gerigk, M.
A1  - Bujnicki, R.
A1  - Ganpo-Nkwenkwa, E.
A1  - Bongaerts, Johannes
A1  - Sprenger, G.
A1  - Takors, Ralf
T1  - Process control for enhanced L-phenylalanine production using different recombinant Escherichia coli strains
JF  - Biotechnology and bioengineering
Y1  - 2002
SN  - 1097-0290 (E-Journal); 0006-3592 (Print)
VL  - Vol. 80
IS  - Iss. 7
SP  - 746
EP  - 754
ER  - 
TY  - JOUR
A1  - Bongaerts, Johannes
A1  - Bovenberg, Roel
A1  - Krämer, Marco
A1  - Müller, Ulrike
A1  - Raeven, Leon
A1  - Wubbolts, Marcel
T1  - Metabolic engineering to produce fine chemicals in Escherichia coli
JF  - Chemie - Ingenieur - Technik (CIT)
Y1  - 2002
SN  - 1522-2640 (E-Journal); 0009-286X (Print)
N1  - Printausg. in der Bibliothek vorhanden: 63 ZS 022
VL  - Vol. 74
IS  - Iss. 5
SP  - 694
ER  - 
TY  - JOUR
A1  - Schmitz, M.
A1  - Hirsch, E.
A1  - Bongaerts, Johannes
A1  - Takors, Ralf
T1  - Pulse experiments as a prerequisite for the quantification of in vivo enzyme kinetics in aromatic amino acid pathway of Eschericia coli
JF  - Biotechnology progress
Y1  - 2002
SN  - 1520-6033 (E-Journal); 8756-7938 (Print)
VL  - Vol. 18
IS  - Iss. 5
SP  - 935
EP  - 941
ER  - 
TY  - JOUR
A1  - Bongaerts, Johannes
A1  - Krämer, Marco
A1  - Müller, Ulrike
A1  - Raeven, Leon
A1  - Wubbolts, Marcel
T1  - Metabolic engineering for microbial production of aromatic amino acids and derived compounds
JF  - Metabolic engineering
Y1  - 2001
SN  - 1096-7184 (E-Journal); 1096-7176 (Print)
VL  - Vol. 3
IS  - Iss. 4
SP  - 289
EP  - 300
ER  - 
TY  - JOUR
A1  - Jossek, Ralf
A1  - Bongaerts, Johannes
A1  - Sprenger, Georg A.
T1  - Characterization of a new feedback-resistant 3-deoxy-D-arabinoheptulosonate 7-phosphate synthase AroF of Escherichia coli
JF  - FEMS microbiology letters
Y1  - 2001
SN  - 1574-6968
VL  - Vol. 202
IS  - Iss. 1
SP  - 145
EP  - 148
ER  - 
TY  - JOUR
A1  - Müller, Ulrike
A1  - Bongaerts, Johannes
A1  - Bovenberg, Roel
A1  - Jossek, Ralf
A1  - Krämer, Marco
A1  - Linnemann, J.
A1  - Müschen, S.
A1  - Ritterbecks, S.
A1  - Sprenger, G.
A1  - Wubbolts, Marcel
T1  - Metabolic engineering to produce fine chemicals in Escherichia coli
JF  - Mededelingen van de Faculteit Landbouwwetenschappen, Rijksuniversiteit Gent
Y1  - 2001
SN  - 0035-533x
VL  - 66 (3a)
SP  - 215
EP  - 217
ER  - 
TY  - JOUR
A1  - Gerigk, M.
A1  - Maaß, D.
A1  - Takors, Ralf
A1  - Kreutzer, A.
A1  - Wandrey, Christian
A1  - Bongaerts, Johannes
A1  - Wubbolts, Marcel
T1  - Fermentative Herstellung von L-Phenylalanin im Fed-Batch Verfahren mit E. coli unter Einbindung eines integrierten Aufarbeitungsverfahrens
JF  - Chemie-Ingenieur-Technik (CIT)
Y1  - 2000
SN  - 1522-2640 (E-Journal); 0009-286X (Print)
N1  - Printausg. in der Bibliothek vorhanden: 63 ZS 022
VL  - Vol. 72
IS  - Iss. 9
SP  - 926
EP  - 927
ER  - 
TY  - JOUR
A1  - Wackwitz, B.
A1  - Bongaerts, Johannes
A1  - Goodman, S. D.
A1  - Unden, Gottfried
T1  - Growth phase-dependent regulation of nuoA-N expression in Escherichia coli K-12 by the Fis protein: upstream binding sites and bioenergetic significance
JF  - Molecular and general genetics : MGG
Y1  - 1999
SN  - 1617-4623 (E-Journal); 1617-4615 (Print)
VL  - Vol. 262
IS  - Iss. 4 - 5
SP  - 876
EP  - 883
ER  - 
TY  - JOUR
A1  - Zientz, Evelyn
A1  - Bongaerts, Johannes
A1  - Unden, Gottfried
T1  - Fumarate regulation of gene expression in Escherichia coli by the DcuSR (dcuSR genes) two-component regulatory system
JF  - Journal of bacteriology
Y1  - 1998
SN  - 1098-5530 (E-Journal); 0021-9193 (Print)
VL  - Vol. 180
IS  - No. 20
SP  - 5421
EP  - 5425
ER  - 
TY  - JOUR
A1  - Unden, Gottfried
A1  - Bongaerts, Johannes
T1  - Alternative respiratory pathways of Escherichia coli: energetics and transcriptional regulation in response to electron acceptors
JF  - Biochimica et biophysica acta (BBA) - Bioenergetics
Y1  - 1997
SN  - 1879-2650 (E-Journal); 0005-2728 (Print)
VL  - Vol. 1320
IS  - Iss. 3
SP  - 217
EP  - 234
ER  - 
TY  - JOUR
A1  - Tran, Quang Hon
A1  - Bongaerts, Johannes
A1  - Vlad, Dorina
A1  - Unden, Gottfried
T1  - Requirement for the proton-pumping NADH dehydrogenase I of Escherichia coli in respiration of NADH to fumarate and its bioenergetic implications
JF  - European journal of biochemistry
Y1  - 1997
SN  - 0014-2956
VL  - Vol. 244
IS  - Iss. 1
SP  - 155
EP  - 160
ER  - 
TY  - JOUR
A1  - Wissenbach, U.
A1  - Six, S.
A1  - Bongaerts, Johannes
A1  - Ternes, D.
A1  - Steinwachs, S.
A1  - Unden, G.
T1  - A third periplasmic transport system for l-arginine in Escherichia coli: molecular characterization of the artPIQMJ genes, arginine binding and transport
JF  - Molecular microbiology
Y1  - 1995
SN  - 1365-2958 (E-Journal); 0950-382x (Print)
VL  - Vol. 17
IS  - Iss. 4
SP  - 675
EP  - 686
ER  - 
TY  - JOUR
A1  - Unden, G.
A1  - Becker, S.
A1  - Bongaerts, Johannes
A1  - Holighaus, G.
A1  - Schirawski, J.
A1  - Six, S.
T1  - O2-sensing and O2-dependent gene regulation in facultatively anaerobic bacteria
JF  - Archives of microbiology
Y1  - 1995
SN  - 1432-072X (E-Journal); 0003-9276 (Print); 0302-8933 (Print)
VL  - Vol. 164
IS  - Iss. 2
SP  - 81
EP  - 90
ER  - 
TY  - JOUR
A1  - Bongaerts, Johannes
A1  - Zoschke, Sascha
A1  - Weidner, Uwe
A1  - Linden, Gottfried
T1  - Transcriptional regulation of the proton translocating NADH
JF  - Molecular microbiology
Y1  - 1995
SN  - 1365-2958 (E-Journal); 0950-382x (Print)
VL  - Vol. 16
IS  - Iss. 3
SP  - 521
EP  - 534
ER  - 
TY  - JOUR
A1  - Unden, G.
A1  - Becker, S.
A1  - Bongaerts, Johannes
A1  - Schirawski, J.
A1  - Six, S.
T1  - Oxygen regulated gene expression in facultatively anaerobic bacteria
JF  - Antonie van Leeuwenhoek
Y1  - 1994
SN  - 0003-6072 (Print) ; 1572-9699 (online)
VL  - Vol. 66
IS  - Iss. 1-3
SP  - 3
EP  - 22
ER  - 
TY  - JOUR
A1  - Goldbach, Daniel
T1  - Fahrstabilitätserhöhung bei Gespannen durch Querverschiebung des Anhängerkupplungspunktes
JF  - mechatronik mobil
Y1  - 2010
SN  - 1867-7371
VL  - 2
IS  - 1
SP  - 23
EP  - 29
PB  - Expert Verlag
CY  - Renningen
ER  - 
TY  - JOUR
A1  - Böhm, Stefan
A1  - Hellmanns, Mark
A1  - Backes, Andreas
A1  - Dilger, Klaus
T1  - Lock-in thermography based NDT of parts for the automotive industry
JF  - Journal of adhesion and interface
Y1  - 2006
VL  - Vol. 7
IS  - No. 4
SP  - 10
EP  - 12
ER  - 
TY  - JOUR
A1  - Hellmanns, Mark
A1  - Böhm, Stefan
A1  - Dilger, Klaus
T1  - Manual applications of adhesives
JF  - Journal of adhesion and interface
Y1  - 2006
VL  - Vol. 7
IS  - No. 4
SP  - 24
EP  - 27
ER  - 
TY  - JOUR
A1  - Laack, Walter van
T1  - Our world is well ordered in measurement and number : or why natural constants are as they are
JF  - American Journal of Humanities and Social Sciences (AHSS)
N2  - All the important natural constants can be logically explained with and derived from the first four ordinal numbers, 1, 2, 3 and 4, its addition to ten and finally the standard values for obviously maximal feasibility Ω and the optimum in our world, the Golden Section (GS), i.e. the number sequences 273 and 618. They both are the first three numbers of irrational results by an arithmetical transformation of simple geometrical relationships by creating multiplicity out of singularity. Both of them show that the infinite is inherent in finiteness and explain in a simple way the smallest deviations and fluctuations between the physical AS-IS state and the obvious spiritual ideal behind: Wherever we look in this world, and especially in important key-positions, we regularly find these sequences. All of the above mentioned numbers so seem to be key players in our world, what can be demonstrated by the derivation of natural constants.
Y1  - 2013
U6  - https://doi.org/10.11634/232907811301390
SN  - 2329-079X
SN  - 2329-0781
VL  - 1
IS  - 4
SP  - 219
EP  - 221
ER  - 
TY  - JOUR
A1  - Schnitzler, Thomas
A1  - Biselli, Manfred
T1  - Cultivo de lineas celulares de hibridoma por CF-10H5 (DSMZ ACC477) en el BIOSTAT B plus
JF  - Noticias tecnicas del laboratorio
Y1  - 2005
VL  - 13
IS  - 3
SP  - 7
EP  - 8
ER  - 
TY  - JOUR
A1  - Schnitzler, Thomas
T1  - Kultivierung der Hybridomazelllinie CF-10H5 (DSMZ ACC477)
JF  - Application notes / Sartorius stedim biotech
Y1  - 2009
PB  - Sartorius Stedim Biotec
CY  - Aubagne
ER  - 
TY  - JOUR
A1  - Schnitzler, Thomas
T1  - Cultivation of hybridoma cell line CF-10H5 (DSMZ ACC477)
JF  - Application notes / Sartorius stedim biotech
Y1  - 2009
SP  - 1
EP  - 4
ER  - 
TY  - JOUR
A1  - Poppel, Kristin Isabel
A1  - Wolf, Martin R.
T1  - Möglichkeiten und Potentiale von Revisionsmarketing
JF  - Zeitschrift Interne Revision : ZIR ; Fachzeitschrift für Wissenschaft und Praxis
Y1  - 2013
SN  - 0044-3816
VL  - 48
IS  - 4
SP  - 200
EP  - 208
PB  - Erich Schmidt Verlag
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Winckler, Silvia
A1  - Krueger, Rolf
A1  - Schnitzler, Thomas
A1  - Zang, Werner
A1  - Fischer, Rainer
A1  - Biselli, Manfred
T1  - A sensitive monitoring system for mammalian cell cultivation processes: a PAT approach
JF  - Bioprocess and biosystems engineering
N2  - Biopharmaceuticals such as antibodies are produced in cultivated mammalian cells, which must be monitored to comply with good manufacturing practice. We, therefore, developed a fully automated system comprising a specific exhaust gas analyzer, inline analytics and a corresponding algorithm to precisely determine the oxygen uptake rate, carbon dioxide evolution rate, carbon dioxide transfer rate, transfer quotient and respiratory quotient without interrupting the ongoing cultivation, in order to assess its reproducibility. The system was verified using chemical simulation experiments and was able to measure the respiratory activity of hybridoma cells and DG44 cells (derived from Chinese hamster ovary cells) with satisfactory results at a minimum viable cell density of ~2.0 × 10⁵ cells ml⁻¹. The system was suitable for both batch and fed-batch cultivations in bubble-aerated and membrane-aerated reactors, with and without the control of pH and dissolved oxygen.
Y1  - 2014
U6  - https://doi.org/10.1007/s00449-013-1062-8
SN  - 1615-7591 (Print) 1615-7605 (Online)
VL  - 37
IS  - 5
SP  - 901
EP  - 912
PB  - Springer
CY  - Berlin, Heidelberg
ER  - 
TY  - JOUR
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Y1  - 2013
SN  - 0219-8762
SP  - Publ. online
PB  - World Scientific Publishing
CY  - Singapore
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TY  - JOUR
A1  - Mischke, Winfried
A1  - Neuß, Frank
T1  - Führt die Übernahme der Domain eines anderen Unternehmers zur Haftung für dessen Schulden nach § 25 HGB?
JF  - Zeitschrift für Vertragsgestaltung, Schuld- und Haftungsrecht : ZGS
Y1  - 2009
SN  - 1619-7011
VL  - 8
SP  - 407
EP  - 409
PB  - ZAP, Verl. f. die Rechts- und Anwaltspraxis
CY  - Recklinghausen
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A1  - Mischke, Winfried
A1  - Girkens, Hans-Peter
T1  - Kostenvorschuss beim selbständigen Beweisverfahren : zur Kostentragung, wenn der Antragsteller den für die Durchführung erforderlichen Vorschuss für den Sachverständigen nicht zahlt
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Y1  - 2011
SN  - 0936-7292
IS  - Fach 13
SP  - 1739
EP  - 1740
PB  - Westlaw Datenbank GmbH
CY  - Frankfurt a. M.
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A1  - Mischke, Winfried
T1  - Die Vernehmung des Wohnungseigentümers : wann ist der Wohnungseigentümer als Partei, wann als Zeuge zu vernehmen?
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Y1  - 2012
SN  - 0936-7292
IS  - Fach 7
SP  - 431
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PB  - ZAP Verlag
CY  - Bonn
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T1  - Why natural constants are as they are
JF  - British journal of arts and social sciences
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SN  - 2046-9578 (E-Journal)
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PB  - BritishJournal Publ. Inc
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T1  - Ist der Tod das Ende? In: Sterben - wie fühlt sich das an / Ines Elsenhans
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SP  - 38
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T1  - Neuerungen bei der Blitzschutznorm. Teil 2
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N1  - Printausg. in der Bibliothek der FH Aachen vorhanden: 23 Z 133 ; 63 ZS 035
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SP  - 66
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SN  - 1873-3859 (E-Journal); 0013-4686 (Print)
SP  - Available online 30.8.2013
PB  - Elsevier
CY  - Amsterdam
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T1  - Syntheses and reactions of urethanes of cellobiose and cellulose-containing uretdione groups
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SN  - 1097-4628
VL  - 44
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SP  - 1043
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A1  - Thrän, Daniela
A1  - Fredebeul-Krein, Markus
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A1  - Seifert, Stefan
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A1  - Böhringer, Christoph
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T1  - CO2-Emissionshandel
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Y1  - 2006
SN  - 0343-5377 (Print) 1866-2765 (Online)
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IS  - Iss. 4
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T1  - Betriebsanweisungen beim Umgang mit Gefahrstoffen
JF  - Elektro-Praktiker : Fachzeitschrift für Handwerk und Industrie
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VL  - Bd. 45
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SP  - 644
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