TY  - JOUR
A1  - Morais, Paulo V.
A1  - Silva, Anielle C. A.
A1  - Dantas, Noelio O.
A1  - Schöning, Michael Josef
A1  - Siqueira, José R., Jr.
T1  - Hybrid Layer‐by‐Layer Film of Polyelectrolytes‐Embedded Catalytic CoFe2O4 Nanocrystals as Sensing Units in Capacitive Electrolyte‐Insulator‐Semiconductor Devices
JF  - physica status solidi a : applications and materials sciences
Y1  - 2019
U6  - http://dx.doi.org/10.1002/pssa.201900044
VL  - 216
IS  - 1900044
SP  - 1
EP  - 9
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Morais, Paulo V.
A1  - Suman, Pedro H.
A1  - Schöning, Michael Josef
A1  - Siqueira Junior, José R.
A1  - Orlandi, Marcelo O.
T1  - Layer-by-layer film based on Sn₃O₄ nanobelts as sensing units to detect heavy metals using a capacitive field-effect sensor platform
JF  - Chemosensors
N2  - Lead and nickel, as heavy metals, are still used in industrial processes, and are classified as “environmental health hazards” due to their toxicity and polluting potential. The detection of heavy metals can prevent environmental pollution at toxic levels that are critical to human health. In this sense, the electrolyte–insulator–semiconductor (EIS) field-effect sensor is an attractive sensing platform concerning the fabrication of reusable and robust sensors to detect such substances. This study is aimed to fabricate a sensing unit on an EIS device based on Sn₃O₄ nanobelts embedded in a polyelectrolyte matrix of polyvinylpyrrolidone (PVP) and polyacrylic acid (PAA) using the layer-by-layer (LbL) technique. The EIS-Sn₃O₄ sensor exhibited enhanced electrochemical performance for detecting Pb²⁺ and Ni²⁺ ions, revealing a higher affinity for Pb²⁺ ions, with sensitivities of ca. 25.8 mV/decade and 2.4 mV/decade, respectively. Such results indicate that Sn₃O₄ nanobelts can contemplate a feasible proof-of-concept capacitive field-effect sensor for heavy metal detection, envisaging other future studies focusing on environmental monitoring.
KW  - Sn₃O₄
KW  - nanobelts
KW  - field-effect sensor
KW  - LbL films
KW  - heavy metals
Y1  - 2023
U6  - http://dx.doi.org/10.3390/chemosensors11080436
SN  - 2227-9040
N1  - This article belongs to the Special Issue The Application of Electrochemical Sensors or Biosensors Based on Nanomaterials
VL  - 11
IS  - 8
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Moreno i Codinachs, L.
A1  - Birkenstock, C.
A1  - Garma, T.
A1  - Zierold, R.
A1  - Bachmann, J.
A1  - Nielsch, K.
A1  - Schöning, Michael Josef
A1  - Fontcuberta i Morral, A.
T1  - A micron-sized nanoporous multifunction sensing device
JF  - physica status solidi (a) . 206 (2009), H. 3
Y1  - 2009
SN  - 1862-6319
N1  - Special Issue: Engineering of Functional Interfaces (EnFI 08)
SP  - 435
EP  - 441
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Moreno i Codinachs, Lia
A1  - Kloock, Joachim P.
A1  - Schöning, Michael Josef
A1  - Baldi, Antoni
A1  - Ipatov, Andrey
A1  - Bratov, Andrey
A1  - Jimenez-Jorquera, Cecilia
T1  - Electronic integrated multisensor tongue applied to grape juice and wine analysis
JF  - Analyst. 133 (2008)
Y1  - 2008
SN  - 1364-5528
SP  - 1440
EP  - 1448
ER  - 
TY  - CHAP
A1  - Moreno, Lia
A1  - Dosev, D.
A1  - Bratov, A.
A1  - Dominguez, C.
A1  - Schöning, Michael Josef
A1  - Kloock, Joachim P.
T1  - Effect of electrical properties of the surrounding medium on the response of an interdigitated electrode array with chalcogenide glass film
T2  - XX Eurosensors : 20th anniversary ; Göteborg, Sweden, 17 - 20 September 2006 ; [proceedings]. - Vol. 1
Y1  - 2006
SN  - 978-91-631-9280-7
N1  - Paper number: T1A-P22
SP  - 384
EP  - 385
CY  - Göteborg
ER  - 
TY  - JOUR
A1  - Moritz, W.
A1  - Yoshinobu, T.
A1  - Finger, F.
A1  - Krause, S.
A1  - Martin-Fernandez, M.
A1  - Schöning, Michael Josef
T1  - High resolution LAPS using amorphous silicon as the semiconductor material
JF  - Sensors and Actuators B. 103 (2004), H. 1-2
Y1  - 2004
SN  - 0925-4005
SP  - 436
EP  - 444
ER  - 
TY  - CHAP
A1  - Moritz, Werner
A1  - Yoshinobu, Tatsuo
A1  - Finger, Friedhelm
A1  - Krause, Steffi
A1  - Schöning, Michael Josef
T1  - Amorphous silicon as semiconductor material for high resolution LAPS
T2  - Eurosensors XVII : the 17th European Conference on Solid-State Transducers ; University of Minho, Guimarães, Portugal, September 21 - 24, 2003
Y1  - 2003
SP  - 48
EP  - 49
ER  - 
TY  - JOUR
A1  - Moseley, Fiona
A1  - Halamek, Jan
A1  - Kramer, Friederike
A1  - Poghossian, Arshak
A1  - Schöning, Michael Josef
A1  - Katz, Evgeny
T1  - An enzyme-based reversible CNOT logic gate realized in a flow system
JF  - Analyst
N2  - An enzyme system organized in a flow device was used to mimic a reversible Controlled NOT (CNOT) gate with two input and two output signals. Reversible conversion of NAD⁺ and NADH cofactors was used to perform a XOR logic operation, while biocatalytic hydrolysis of p-nitrophenyl phosphate resulted in an Identity operation working in parallel. The first biomolecular realization of a CNOT gate is promising for integration into complex biomolecular networks and future biosensor/biomedical applications.
Y1  - 2014
U6  - http://dx.doi.org/10.1039/C4AN00133H
SN  - 1364-5528 (E-Journal) ; 0003-2654 (Print)
VL  - 139
IS  - 8
SP  - 1839
EP  - 1842
PB  - Royal Society of Chemistry
CY  - Cambridge
ER  - 
TY  - JOUR
A1  - Mourzina, I.G.
A1  - Yoshinobu, T.
A1  - Ermolenko, Y.E.
A1  - Vlasov, Y. G.
A1  - Schöning, Michael Josef
A1  - Iwasaki, H.
T1  - Immobilization of urease and cholinesterase on the surface of semiconductor transducer for the development of light-addressable potentiometric sensors
JF  - Technical digest of the 10th International Meeting on Chemical Sensors, July 11 - 14, 2004, Tsukuba, Japan / Japan Association of Chemical Sensors
Y1  - 2004
N1  - Chemical sensors ; 20.2004 Suppl. B. IMCS ; (10, 2004, Tsukuba) ; International Meeting on Chemical Sensors ; (10 : ; 2004.07.11-14 : ; Tsukuba)
SP  - 788
EP  - 789
PB  - Japan Association of Chemical Sensors
CY  - Fukuoka
ER  - 
TY  - JOUR
A1  - Mourzina, Ioulia G.
A1  - Yoshinobu, Tatsuo
A1  - Ermolenko, Yuri E.
A1  - Vlasov, Yuri G.
A1  - Schöning, Michael Josef
A1  - Iwasaki, H.
T1  - Immobilization of urease and cholinesterase on the surface of semiconductor transducer for the development of lightaddressable potentiometric sensors
JF  - Microchimica Acta. 144 (2004), H. 1-3
Y1  - 2004
SN  - 0026-3672
SP  - 41
EP  - 50
ER  - 
TY  - JOUR
A1  - Mourzina, Y. G.
A1  - Ermelenko, Y. E.
A1  - Yoshinobu, T.
A1  - Vlasov, Y.
A1  - Iwasaki, H.
A1  - Schöning, Michael Josef
T1  - Anionselective light-addressable potentiometric sensors (LAPS) for the determination of nitrate and suphate ions
JF  - Sensors and Actuators B. 91 (2003), H. 1-3
Y1  - 2003
SN  - 0925-4005
SP  - 32
EP  - 38
ER  - 
TY  - JOUR
A1  - Mourzina, Y. G.
A1  - Legin, A.
A1  - Vlasov, Y. G.
A1  - Schöning, Michael Josef
A1  - Schubert, J.
A1  - Zander, W.
A1  - Lüth, H.
T1  - Thin film chemical sensors based on chalcogenide glasses for „electronic tongue“ applications
JF  - Sensor 2001 : 10th international conference, May 8 - 10, 2001, Exhibition Centre Nuremberg, Germany ; proceedings
Y1  - 2001
N1  - Sensor-Kongress ; (10, 2001, Nürnberg) Sensor 2001 Conference ; (10 : ; 2001.05.08-10 : ; Nuremberg)
SP  - 137
EP  - 141
PB  - AMA Service
CY  - Wunstorf
ER  - 
TY  - JOUR
A1  - Mourzina, Y. G.
A1  - Schöning, Michael Josef
A1  - Schubert, J.
A1  - Zander, W.
A1  - Legin, A. V.
A1  - Vlasov, Y. G.
A1  - Lüth, H.
T1  - Copper, cadmium and thallium thin film sensors based on chalcogenide glasses
JF  - Analytica Chimica Acta. 433 (2001)
Y1  - 2001
SN  - 0378-4304
SP  - 103
EP  - 110
ER  - 
TY  - JOUR
A1  - Mourzina, Y. G.
A1  - Yoshinobu, T.
A1  - Schubert, J.
A1  - Lüth, H.
A1  - Iwasaki, H.
A1  - Schöning, Michael Josef
T1  - Ion-selective lightaddressable potentiometric sensor (LAPS) with chalcogenide thin film by pulsed laser deposition
JF  - Sensors and Actuators B. 80 (2001), H. 2
Y1  - 2001
SN  - 0925-4005
SP  - 136
EP  - 140
ER  - 
TY  - JOUR
A1  - Mourzina, Y.
A1  - Mai, T.
A1  - Poghossian, Arshak
A1  - Ermolenko, Y.
A1  - Yoshinobu, T.
A1  - Vlasov, Y.
A1  - Iwasaki, H.
A1  - Schöning, Michael Josef
T1  - K+-selective field-effect sensors as transducers for bioelectronic applications
JF  - Electrochimica Acta. 48 (2003), H. 20-22
Y1  - 2003
SN  - 0013-4686
SP  - 3333
EP  - 3339
ER  - 
TY  - JOUR
A1  - Mourzina, Y.
A1  - Yoshinobu, T.
A1  - Ermelenko, Y.
A1  - Furuichi, K.
A1  - Vlasov, Y.
A1  - Schöning, Michael Josef
A1  - Iwasaki, H.
T1  - Technology of photocurable membranes for ion sensors based on laser scanned semiconductor transducer (LSST)
JF  - Book of abstracts / ed. by J. Saneistr.
Y1  - 2002
SN  - 80-01-02576-4
N1  - Eurosensors ; (16, 2002, Praha)
SP  - 254
EP  - 257
PB  - Czech Technical University, Faculty of Electrical Engineering, Department of Measurement
CY  - Prague
ER  - 
TY  - JOUR
A1  - Mourzina, Y.G.
A1  - Schöning, Michael Josef
A1  - Schubert, J.
A1  - Zander, W.
A1  - Legin, A. V.
A1  - Vlasov, Y. G.
A1  - Kordos, P.
A1  - Lüth, H.
T1  - A new thin film Pb microsensor based on chalcogenide glasses
JF  - Sensors and Actuators B. 71 (2000), H. 1-2
Y1  - 2000
SN  - 0925-4005
SP  - 13
EP  - 18
ER  - 
TY  - JOUR
A1  - Mourzina, Yu. G.
A1  - Schubert, J
A1  - Zander, W.
A1  - Legin, A.
A1  - Vlasov, Y. G.
A1  - Lüth, H.
A1  - Schöning, Michael Josef
T1  - Development of multisensor systems based on chalcogenide thin film chemical sensors for the simultaneous multicomponent analysis of metal ions in complex solutions
JF  - Scaling down in electrochemistry : electrochemical micro- and nanosystem technology ; proceedings of the 3rd International Symposium on Electrochemical Microsystem Technologies, Garmisch-Patenkirchen, Germany, 11 - 15 September 2000 / ed. by J. W. Schultz
Y1  - 2001
SN  - 0-08-044014-2
SP  - 251
EP  - 263
PB  - Elsevier [u.a.]
CY  - Amsterdam [u.a.]
ER  - 
TY  - JOUR
A1  - Mourzina, Yu.G.
A1  - Schöning, Michael Josef
A1  - Schubert, J.
A1  - Zander, W.
A1  - Legin, A.
A1  - Vlasov, Y. G.
A1  - Lüth, H.
T1  - Thin film microsensors for fast heavy metal analysis
JF  - Proceedings : Copenhagen, Denmark, 27 - 30 August 2000 / [ed.: R. de Reus ...]
Y1  - 2000
SN  - 87-89935-50-0
N1  - Eurosensors ; (14, 2000, København) ; Eurosensors ; (14 : ; 2000.08.27-30 : ; Copenhagen) ; European Conference on Solid-State Transducers ; (14 : ; 2000.08.27-30 : ; Copenhagen)
SP  - 839
EP  - 840
PB  - MIC, Mikroelektronik Centret
CY  - Lyngby, Denmark
ER  - 
TY  - JOUR
A1  - Muck, A.
A1  - Wang, J.
A1  - Jacobs, M.
A1  - Chen, G.
A1  - Chatrathi, M. P.
A1  - Jurka, V.
A1  - Vyborny, Z.
A1  - Spillmann, S. D.
A1  - Sridharan, G.
A1  - Schöning, Michael Josef
T1  - Fabrication of poly(methyl methacrylate) microfluidic chips by atmospheric molding
JF  - Analytical Chemistry. 76 (2004), H. 8
Y1  - 2004
SN  - 0003-2700
SP  - 2290
EP  - 2297
ER  - 
TY  - JOUR
A1  - Murib, M. S.
A1  - Grinsven, B. van
A1  - Grieten, L.
A1  - Janssens, S. D.
A1  - Vermeeren, V.
A1  - Eersels, K.
A1  - Broeders, J.
A1  - Ameloot, M.
A1  - Michiels, L.
A1  - Ceuninck, W. De
A1  - Haenen, K.
A1  - Schöning, Michael Josef
A1  - Wagner, Patrick
T1  - Electronic monitoring of chemical DNA denaturation on nanocrystalline diamond electrodes with different molarities and flow rates
JF  - Physica Status Solidi (A). Vol. 210 (2013), iss. 5
Y1  - 2013
SN  - 0031-8965
SP  - 911
EP  - 917
PB  - Wiley-VCH
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Murib, M. S.
A1  - Yeap, W. S.
A1  - Eurlings, Y.
A1  - Grinsven, B. van
A1  - Boyen, H.-G.
A1  - Conings, B.
A1  - Michiels, L.
A1  - Ameloot, M.
A1  - Carleer, R.
A1  - Warmer, J.
A1  - Kaul, P.
A1  - Haenen, K.
A1  - Schöning, Michael Josef
A1  - Ceuninck, W. de
A1  - Wagner, P.
T1  - Heat-transfer based characterization of DNA on synthetic sapphire chips
JF  - Sensors and Actuators B: Chemical
N2  - In this study, we show that synthetic sapphire (Al₂O₃), an established implant material, can also serve as a platform material for biosensors comparable to nanocrystalline diamond. Sapphire chips, beads, and powder were first modified with (3-aminopropyl) triethoxysilane (APTES), followed by succinic anhydride (SA), and finally single-stranded probe DNA was EDC coupled to the functionalized layer. The presence of the APTES-SA layer on sapphire powders was confirmed by thermogravimetric analyis and Fourier-transform infrared spectroscopy. Using planar sapphire chips as substrates and X-ray photoelectron spectroscopy (XPS) as surface-sensitive tool, the sequence of individual layers was analyzed with respect to their chemical state, enabling the quantification of areal densities of the involved molecular units. Fluorescence microscopy was used to demonstrate the hybridization of fluorescently tagged target DNA to the probe DNA, including denaturation- and re-hybridization experiments. Due to its high thermal conductivity, synthetic sapphire is especially suitable as a chip material for the heat-transfer method, which was employed to distinguish complementary- and non-complementary DNA duplexes containing single-nucleotide polymorphisms. These results indicate that it is possible to detect mutations electronically with a chemically resilient and electrically insulating chip material.
Y1  - 2016
U6  - http://dx.doi.org/10.1016/j.snb.2016.02.027
SN  - 0925-4005
VL  - 230
IS  - 230
SP  - 260
EP  - 271
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Murib, M. S.
A1  - Yeap, W. S.
A1  - Martens, D.
A1  - Liu, X.
A1  - Bienstman, P.
A1  - Fahlman, M.
A1  - Schöning, Michael Josef
A1  - Michiels, L.
A1  - Haenen, K.
A1  - Serpengüzel, A.
A1  - Wagner, Patrick
T1  - Photonic studies on polymer-coated sapphire-spheres : a model system for biological ligands
JF  - Sensors and actuators A: Physical
N2  - In this study we show an optical biosensor concept, based on elastic light scattering from sapphire microspheres. Transmitted and elastic scattering intensity of the microspheres (radius 500 μm, refractive index 1.77) on an optical fiber half coupler is analyzed at 1510 nm. The 0.43 nm angular mode spacing of the resonances is comparable to the angular mode spacing value estimated using the optical size of the microsphere. The spectral linewidths of the resonances are in the order of 0.01 nm, which corresponds to quality factors of approximately 105. A polydopamine layer is used as a functionalizing agent on sapphire microspherical resonators in view of biosensor implementation. The varying layer thickness on the microsphere is determined as a function of the resonance wavelength shift. It is shown that polymer functionalization has a minor effect on the quality factor. This is a promising step toward the development of an optical biosensor.
Y1  - 2015
U6  - http://dx.doi.org/10.1016/j.sna.2014.11.024
SN  - 1873-3069 (E-Journal); 0924-4247 (Print)
VL  - 222
SP  - 212
EP  - 219
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Murib, Mohammed S.
A1  - Tran, Anh Quang
A1  - Ceuninck, Ward de
A1  - Schöning, Michael Josef
A1  - Nesladek, Milos
A1  - Serpengüzel, Ali
A1  - Wagner, Patrick
T1  - Analysis of an optical biosensor based on elastic light scattering from diamond-, glass-, and sapphire microspheres
JF  - Physica Status Solidi A
N2  - Deoxyribonucleic acid (DNA) and protein recognition are now standard tools in biology. In addition, the special optical properties of microsphere resonators expressed by the high quality factor (Q-factor) of whispering gallery modes (WGMs) or morphology dependent resonances (MDRs) have attracted the attention of the biophotonic community. Microsphere-based biosensors are considered as powerful candidates to achieve label-free recognition of single molecules due to the high sensitivity of their WGMs. When the microsphere surface is modified with biomolecules, the effective refractive index and the effective size of the microsphere change resulting in a resonant wavelength shift. The transverse electric (TE) and the transverse magnetic (TM) elastic light scattering intensity of electromagnetic waves at 600 and 1400 nm are numerically calculated for DNA and unspecific binding of proteins to the microsphere surface. The effect of changing the optical properties was studied for diamond (refractive index 2.34), glass (refractive index 1.50), and sapphire (refractive index 1.75) microspheres with a 50 µm radius. The mode spacing, the linewidth of WGMs, and the shift of resonant wavelength due to the change in radius and refractive index, were analyzed by numerical simulations. Preliminary results of unspecific binding of biomolecules are presented. The calculated shift in WGMs can be used for biomolecules detection.
Y1  - 2012
U6  - http://dx.doi.org/10.1002/pssa.201100795
SN  - 1862-6319
N1  - Special Issue: "Fundamentals and Applications of Diamond"
VL  - 209
IS  - 9
SP  - 1804
EP  - 1810
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Muschallik, Lukas
A1  - Kipp, Carina Ronja
A1  - Recker, Inga
A1  - Bongaerts, Johannes
A1  - Pohl, Martina
A1  - Gelissen, Melanie
A1  - Schöning, Michael Josef
A1  - Selmer, Thorsten
A1  - Siegert, Petra
T1  - Synthesis of α-hydroxy ketones and vicinal diols with the Bacillus licheniformis DSM 13T butane-2, 3-diol dehydrogenase
JF  - Journal of Biotechnology
N2  - The enantioselective synthesis of α-hydroxy ketones and vicinal diols is an intriguing field because of the broad applicability of these molecules. Although, butandiol dehydrogenases are known to play a key role in the production of 2,3-butandiol, their potential as biocatalysts is still not well studied. Here, we investigate the biocatalytic properties of the meso-butanediol dehydrogenase from Bacillus licheniformis DSM 13T (BlBDH). The encoding gene was cloned with an N-terminal StrepII-tag and recombinantly overexpressed in E. coli. BlBDH is highly active towards several non-physiological diketones and α-hydroxyketones with varying aliphatic chain lengths or even containing phenyl moieties. By adjusting the reaction parameters in biotransformations the formation of either the α-hydroxyketone intermediate or the diol can be controlled.
Y1  - 2020
SN  - 2590-1559
U6  - http://dx.doi.org/10.1016/j.jbiotec.2020.09.016
VL  - 202
IS  - Vol. 324
SP  - 61
EP  - 70
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Muschallik, Lukas
A1  - Molinnus, Denise
A1  - Bongaerts, Johannes
A1  - Pohl, Martina
A1  - Wagner, Torsten
A1  - Schöning, Michael Josef
A1  - Siegert, Petra
A1  - Selmer, Thorsten
T1  - (R,R)-Butane-2,3-diol Dehydrogenase from Bacillus clausii DSM 8716T: Cloning and Expression of the bdhA-Gene, and Initial Characterization of Enzyme
JF  - Journal of Biotechnology
N2  - The gene encoding a putative (R,R)-butane-2,3-diol dehydrogenase (bdhA) from Bacillus clausii DSM 8716T was isolated, sequenced and expressed in Escherichia coli. The amino acid sequence of the encoded protein is only distantly related to previously studied enzymes (identity 33–43%) and exhibited some uncharted peculiarities. An N-terminally StrepII-tagged enzyme variant was purified and initially characterized. The isolated enzyme catalyzed the (R)-specific oxidation of (R,R)- and meso-butane-2,3-diol to (R)- and (S)-acetoin with specific activities of 12 U/mg and 23 U/mg, respectively. Likewise, racemic acetoin was reduced with a specific activity of up to 115 U/mg yielding a mixture of (R,R)- and meso-butane-2,3-diol, while the enzyme reduced butane-2,3-dione (Vmax 74 U/mg) solely to (R,R)-butane-2,3-diol via (R)-acetoin. For these reactions only activity with the co-substrates NADH/NAD+ was observed. The enzyme accepted a selection of vicinal diketones, α-hydroxy ketones and vicinal diols as alternative substrates. Although the physiological function of the enzyme in B. clausii remains elusive, the data presented herein clearly demonstrates that the encoded enzyme is a genuine (R,R)-butane-2,3-diol dehydrogenase with potential for applications in biocatalysis and sensor development.
Y1  - 2017
U6  - http://dx.doi.org/10.1016/j.jbiotec.2017.07.020
SN  - 0168-1656
VL  - 258
SP  - 41
EP  - 50
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Muschallik, Lukas
A1  - Molinnus, Denise
A1  - Jablonski, Melanie
A1  - Kipp, Carina Ronja
A1  - Bongaerts, Johannes
A1  - Pohl, Martina
A1  - Wagner, Torsten
A1  - Schöning, Michael Josef
A1  - Selmer, Thorsten
A1  - Siegert, Petra
T1  - Synthesis of α-hydroxy ketones and vicinal (R, R)-diols by Bacillus clausii DSM 8716ᵀ butanediol dehydrogenase
JF  - RSC Advances
N2  - α-hydroxy ketones (HK) and 1,2-diols are important building blocks for fine chemical synthesis. Here, we describe the R-selective 2,3-butanediol dehydrogenase from B. clausii DSM 8716ᵀ (BcBDH) that belongs to the metal-dependent medium chain dehydrogenases/reductases family (MDR) and catalyzes the selective asymmetric reduction of prochiral 1,2-diketones to the corresponding HK and, in some cases, the reduction of the same to the corresponding 1,2-diols. Aliphatic diketones, like 2,3-pentanedione, 2,3-hexanedione, 5-methyl-2,3-hexanedione, 3,4-hexanedione and 2,3-heptanedione are well transformed. In addition, surprisingly alkyl phenyl dicarbonyls, like 2-hydroxy-1-phenylpropan-1-one and phenylglyoxal are accepted, whereas their derivatives with two phenyl groups are not substrates. Supplementation of Mn²⁺ (1 mM) increases BcBDH's activity in biotransformations. Furthermore, the biocatalytic reduction of 5-methyl-2,3-hexanedione to mainly 5-methyl-3-hydroxy-2-hexanone with only small amounts of 5-methyl-2-hydroxy-3-hexanone within an enzyme membrane reactor is demonstrated.
Y1  - 2020
U6  - http://dx.doi.org/10.1039/D0RA02066D
SN  - 2046-2069
VL  - 10
SP  - 12206
EP  - 12216
PB  - Royal Society of Chemistry (RSC)
CY  - Cambridge
ER  - 
TY  - JOUR
A1  - Näther, Niko
A1  - Auger, V.
A1  - Poghossian, Arshak
A1  - Koudelka-Hep, M.
A1  - Schöning, Michael Josef
T1  - A miniaturized flow-through cell in SU-8 technique for EIS sensors
JF  - Biomedizinische Technik. 49 (2004), H. 2
Y1  - 2004
SN  - 0932-4666
SP  - 994
EP  - 995
ER  - 
TY  - JOUR
A1  - Näther, Niko
A1  - Berger, Jörg
A1  - Emmerich, Rüdiger
A1  - Friedrich, P.
A1  - Henkel, H.
A1  - Schöning, Michael Josef
T1  - H2O2-Konzentrationsbestimmung für die Prozessgasanalytik mittels eines kalorimetrischen Sensoraufbaus
JF  - Sensoren und Messsysteme 2008 : 14. Fachtagung Ludwigsburg, 11. und 12. März 2008 / VDI/VDE-Gesellschaft Mess- und Automatisierungstechnik
Y1  - 2008
SN  - 978-3-18-092011-5
N1  - VDI-Berichte ; 2011 ; Sensoren und Messsysteme 2008, 14. GMA/ITG-Fachtagung, VDI/VDE- Gesellschaft Mess- und Automatisierungstechnik, + CD-ROM, Ludwigsburg, DE, 11.-12. Mar, 2008
SP  - 783
EP  - 792
PB  - VDI-Verl.
CY  - Düsseldorf
ER  - 
TY  - JOUR
A1  - Näther, Niko
A1  - Berger, Jörg
A1  - Emmerich, Rüdiger
A1  - Friedrich, Peter
A1  - Henkel, Hartmut
A1  - Schneider, Andreas
A1  - Schöning, Michael Josef
T1  - Entwicklung einer kalorimetrischen H2O2-Gassensoranordnung für die Prozessgaskontrolle
JF  - 8. Dresdner Sensor-Symposium : Sensoren für Umwelt, Klima und Sicherheit, Biosensoren und Biosysteme, Sensoren und Sensorsysteme für die Prozesstechnik, Trends in der Sensortechnik, Materialentwicklung für die Sensorik; 8. Dresdner Sensor-Symposium, 10. - 12. Dezember 2007, Dresden / Gerald Gerlach ... (Hg.)
Y1  - 2007
SN  - 978-3-940046-45-1
N1  - Dresdner Beiträge zur Sensorik ; 29
SP  - 261
EP  - 264
PB  - TUDpress, Verl. der Wissenschaften
CY  - Dresden
ER  - 
TY  - JOUR
A1  - Näther, Niko
A1  - Emmerich, Rüdiger
A1  - Berger, Jörg
A1  - Friedrich, Peter
A1  - Henkel, Hartmut
A1  - Schneider, Andreas
A1  - Schöning, Michael Josef
T1  - A novel gas-phase hydrogen peroxide sensor basing on a combined physical/chemical transduction mechanism
JF  - Nanofunctional materials, nanostructures, and novel devices for biological and chemical detection : November 27 - December 1, 2006, Boston, Massachusetts, USA ; [at the 2006 MRS Fall Meeting].
Y1  - 2006
SN  - 978-1-60423-407-7
N1  - Materials Research Society symposium proceedings ; 951 ; MRS fall meeting ; (2006.11.27-12.01 : ; Boston, Mass.)
SP  - 63
EP  - 68
PB  - Materials Research Soc.
CY  - Warrendale, Pa.
ER  - 
TY  - JOUR
A1  - Näther, Niko
A1  - Henkel, Hartmut
A1  - Schneider, Andreas
A1  - Schöning, Michael Josef
T1  - Investigation of different catalytically active and passive materials for realising a hydrogen peroxide gas sensor
JF  - physica status solidi (a) . 206 (2009), H. 3
Y1  - 2009
SN  - 1862-6319
N1  - Special Issue: Engineering of Functional Interfaces (EnFI 08)
SP  - 449
EP  - 454
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - CHAP
A1  - Näther, Niko
A1  - Juárez, Leon M.
A1  - Emmerich, Rüdiger
A1  - Berger, Jörg
A1  - Friedrich, Peter
A1  - Schöning, Michael Josef
T1  - Detection of hydrogen peroxide (H2O2) at exposed temperatures for industrial processes
N2  - An H2O2 sensor for the application in industrial sterilisation processes has been developed. Therefore, automated sterilisation equipment at laboratory scale has been constructed using parts from industrial sterilisation facilities. In addition, a software tool has been developed for the control of the sterilisation equipment at laboratory scale. First measurements with the developed sensor set-up as part of the sterilisation equipment have been performed and the sensor has been physically characterised by optical microscopy and SEM.
KW  - Biosensor
KW  - Gas sensor
KW  - hydrogen peroxide
KW  - sterilisation
KW  - catalytic decomposition
Y1  - 2006
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:hbz:a96-opus-1418
ER  - 
TY  - CHAP
A1  - Näther, Niko
A1  - Poghossian, Arshak
A1  - Platen, J.
A1  - Yoshinobu, T.
A1  - Koudelka-Hep, M.
A1  - Schöning, Michael Josef
T1  - Multi-parameter sensing of both physical and (bio-)chemical quantities using the same transducer principle
T2  - Biochemical sensing utilisation of micro- and nanotechnologies : Warsaw, [23rd - 26th] November 2005 / ed. by M. Mascini ...
Y1  - 2006
SP  - 172
EP  - 181
CY  - Warsaw
ER  - 
TY  - JOUR
A1  - Näther, Niko
A1  - Rolka, David
A1  - Poghossian, Arshak
A1  - Koudelka-Hep, M.
A1  - Schöning, Michael Josef
T1  - Two microcell flow-injection analysis (FIA) platforms for capacitive silicon-based field-effect sensors
JF  - Electrochimica Acta. 51 (2005), H. 5
Y1  - 2005
SN  - 0013-4686
U6  - http://dx.doi.org/10.1016/j.electacta.2005.04.066
SP  - 924
EP  - 929
ER  - 
TY  - CHAP
A1  - Oberländer, Jan
A1  - Arreola, Julio
A1  - Hansen, Christina
A1  - Greeff, Anton
A1  - Mayer, Marlena
A1  - Keusgen, Michael
A1  - Schöning, Michael Josef
T1  - Impedimetric Biosensor to Enable Fast Evaluation of Gaseous Sterilization Processes
T2  - MDPI Proceedings
Y1  - 2017
U6  - http://dx.doi.org/10.3390/proceedings1040435
N1  - Eurosensors 2017 Conference, Paris, France, 3–6 September 2017
VL  - 1
IS  - 4
ER  - 
TY  - JOUR
A1  - Oberländer, Jan
A1  - Bromm, Alexander
A1  - Wendeler, Luisa
A1  - Iken, Heiko
A1  - Palomar Duran, Marlena
A1  - Greeff, Anton
A1  - Kirchner, Patrick
A1  - Keusgen, Michael
A1  - Schöning, Michael Josef
T1  - Towards a biosensor to monitor the sterilisation efficiency of aseptic filling machines
JF  - Physica status solidi (a)
N2  - Sterilisation processes are compulsory in medicine, pharmacy, and food industries to prevent infections of consumers and microbiological contaminations of products. Monitoring the sterilisation by conventional microbiological methods is time- and lab-consuming. To overcome this problem, in this work a novel biosensor has been proposed. The sensor enables a fast method to evaluate sterilisation processes. By means of thin-film technology the sensor's transducer structures in form of IDEs (interdigitated electrodes) have been fabricated on a silicon substrate. Physical characterisation of the developed sensor was done by AFM, SEM, and profilometry. Impedance analyses were conducted for the electrical characterisation. As microbiological layer spores of B. atrophaeus have been immobilised on the sensing structure; spores of this type are a well-known sterilisation test organism. Impedance measurements at a fixed frequency over time were performed to monitor the immobilisation process. A sterilisation process according to aseptic filling machines was applied to demonstrate the sensor functionality. After both, immobilisation and sterilisation, a change in impedance could successfully be detected.
Y1  - 2015
U6  - http://dx.doi.org/10.1002/pssa.201431900
SN  - 1862-6319
VL  - 212
IS  - 6
SP  - 1299
EP  - 1305
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Oberländer, Jan
A1  - Jildeh, Zaid B.
A1  - Kirchner, Patrick
A1  - Wendeler, Luisa
A1  - Bromm, Alexander
A1  - Iken, Heiko
A1  - Wagner, Patrick
A1  - Keusgen, Michael
A1  - Schöning, Michael Josef
T1  - Study of Interdigitated Electrode Arrays Using Experiments and Finite Element Models for the Evaluation of Sterilization Processes
JF  - Sensors
N2  - In this work, a sensor to evaluate sterilization processes with hydrogen peroxide vapor has been characterized. Experimental, analytical and numerical methods were applied to evaluate and study the sensor behavior. The sensor set-up is based on planar interdigitated electrodes. The interdigitated electrode structure consists of 614 electrode fingers spanning over a total sensing area of 20 mm2. Sensor measurements were conducted with and without microbiological spores as well as after an industrial sterilization protocol. The measurements were verified using an analytical expression based on a first-order elliptical integral. A model based on the finite element method with periodic boundary conditions in two dimensions was developed and utilized to validate the experimental findings.
Y1  - 2015
U6  - http://dx.doi.org/10.3390/s151026115
SN  - 1424-8220
N1  - This article belongs to the Special Issue "Gas Sensors—Designs and Applications"
VL  - 15
IS  - 10
SP  - 26115
EP  - 26127
PB  - MDPI
CY  - Basel
ER  - 
TY  - CHAP
A1  - Oberländer, Jan
A1  - Jildeh, Zaid B.
A1  - Kirchner, Patrick
A1  - Wendeler, Luisa
A1  - Bromm, Alexander
A1  - Iken, Heiko
A1  - Wagner, Patrick
A1  - Keusgen, Michael
A1  - Schöning, Michael Josef
T1  - Experimental and numerical evaluation of interdigitated electrode array for monitoring gaseous sterilization processes
T2  - 12. Dresdner Sensor-Symposium 2015
Y1  - 2015
U6  - http://dx.doi.org/10.5162/12dss2015/P3.11
SP  - 163
EP  - 168
ER  - 
TY  - JOUR
A1  - Oberländer, Jan
A1  - Kirchner, Patrick
A1  - Boyen, Hans-Gerd
A1  - Schöning, Michael Josef
T1  - Detection of hydrogen peroxide vapor by use of manganese(IV) oxide as catalyst for calorimetric gas sensors
JF  - Physica status solidi A: Applications and materials science
N2  - In this work, the catalyst manganese(IV) oxide (MnO2), of calorimetric gas sensors (to monitor the sterilization agent vaporized hydrogen peroxide) has been investigated in more detail. Chemical analyses by means of X-ray-induced photoelectron spectroscopy have been performed to unravel the surface chemistry prior and after exposure to hydrogen peroxide vapor at elevated temperature, as applied in the sterilization processes of beverage cartons. The surface characterization reveals a change in oxidation states of the metal oxide catalyst after exposure to hydrogen peroxide. Additionally, a cleaning effect of the catalyst, which itself is attached to the sensor surface by means of a polymer interlayer, could be observed.
Y1  - 2014
U6  - http://dx.doi.org/10.1002/pssa.201330359
SN  - 1521-396X (E-Journal); 1862-6319 (E-Journal); 0031-8965 (Print); 1862-6300 (Print)
VL  - 211
IS  - 6
SP  - 1372
EP  - 1376
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - CHAP
A1  - Oberländer, Jan
A1  - Kirchner, Patrick
A1  - Keusgen, M.
A1  - Schöning, Michael Josef
T1  - Flexible polyimide-based calorimetric gas sensors for monitoring hy-drogen peroxide in sterilisation processes of aseptic filling machines
T2  - Sensoren und Messsysteme 2014 ; Beiträge der 17. GMA/ITG-Fachtagung vom 3. bis 4. Juni 2014 in Nürnberg. (ITG-Fachbericht ; 250)
Y1  - 2014
SN  - 978-3-8007-3622-5
SP  - 1
EP  - 4
PB  - VDE-Verl.
CY  - Düsseldorf
ER  - 
TY  - JOUR
A1  - Oberländer, Jan
A1  - Kirchner, Patrick
A1  - Keusgen, Michael
A1  - Schöning, Michael Josef
T1  - Strategies in developing thin-film sensors for monitoring aseptic food processes : Theoretical considerations and investigations of passivation materials
JF  - Electrochimica Acta
N2  - The sterilization of packages in aseptic food processes is highly significant to maintain a consumer-safe product with extended shelf-life. Today, the sterilization of food packages is predominantly accomplished by gaseous hydrogen peroxide (H2O2) in combination with heat. In order to monitor this sterilization process, calorimetric gas sensors as differential set-up of two platinum temperature sensors representing a catalytically active (additionally deposition of MnO2) and a passive segment have been recently developed. The temperature rise of the exothermic decomposition serves as an indicator of the present H2O2 concentration. In the present work, a theoretical approach considering the sensor’s thermochemistry and physical transport phenomena was formulated to evaluate the temperature rise based on the energy content of gaseous H2O2. In a further part of this work, three polymers have been analyzed with respect to their application as passivation materials. The examined polymers are photoresist SU-8, perfluoroalkoxy (PFA) and fluorinated ethylene propylene (FEP). Thermal analyses by means of differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA) have been conducted to determine the operation limits of the polymers. The overall chemical resistance and stability of the polymers against the harsh environmental conditions during the sterilization process have been examined by attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR).
Y1  - 2015
U6  - http://dx.doi.org/10.1016/j.electacta.2015.06.126
SN  - 0013-4686
VL  - 183
SP  - 130
EP  - 136
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Oberländer, Jan
A1  - Mayer, Marlena
A1  - Greeff, Anton
A1  - Keusgen, Michael
A1  - Schöning, Michael Josef
T1  - Spore-based biosensor to monitor the microbicidal efficacy of gaseous hydrogen peroxide sterilization processes
JF  - Biosensors and Bioelectronics
N2  - In this work, a spore-based biosensor is evaluated to monitor the microbicidal efficacy of sterilization processes applying gaseous hydrogen peroxide (H2O2). The sensor is based on interdigitated electrode structures (IDEs) that have been fabricated by means of thin-film technologies. Impedimetric measurements are applied to study the effect of sterilization process on spores of Bacillus atrophaeus. This resilient microorganism is commonly used in industry to proof the sterilization efficiency. The sensor measurements are accompanied by conventional microbiological challenge tests, as well as morphological characterizations with scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The sensor measurements are correlated with the microbiological test routines. In both methods, namely the sensor-based and microbiological one, a tailing effect has been observed. The results are evaluated and discussed in a three-dimensional calibration plot demonstrating the sensor's suitability to enable a rapid process decision in terms of a successfully performed sterilization.
Y1  - 2018
U6  - http://dx.doi.org/10.1016/j.bios.2017.12.045
SN  - 0956-5663
VL  - 104
SP  - 87
EP  - 94
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - CHAP
A1  - Oberländer, Jan
A1  - Reisert, Steffen
A1  - Kirchner, Patrick
A1  - Wagner, Patrick
A1  - Schöning, Michael Josef
T1  - Kalorimetrische Gassensoren zur H2O2-Detektion in aseptischen Sterilisationsprozessen
T2  - 11. Dresdner Sensor-Symposium : 9.-11.12.2013
Y1  - 2013
SN  - 978-3-9813484-5-3
SP  - 234
EP  - 238
ER  - 
TY  - JOUR
A1  - Oliveira, Danilo A.
A1  - Molinnus, Denise
A1  - Beging, Stefan
A1  - Siqueira Jr, José R.
A1  - Schöning, Michael Josef
T1  - Biosensor Based on Self-Assembled Films of Graphene Oxide and Polyaniline Using a Field-Effect Device Platform
JF  - physica status solidi (a) applications and materials science
N2  - A new functionalization method to modify capacitive electrolyte–insulator–semiconductor (EIS) structures with nanofilms is presented. Layers of polyallylamine hydrochloride (PAH) and graphene oxide (GO) with the compound polyaniline:poly(2-acrylamido-2-methyl-1-propanesulfonic acid) (PANI:PAAMPSA) are deposited onto a p-Si/SiO2 chip using the layer-by-layer technique (LbL). Two different enzymes (urease and penicillinase) are separately immobilized on top of a five-bilayer stack of the PAH:GO/PANI:PAAMPSA-modified EIS chip, forming a biosensor for detection of urea and penicillin, respectively. Electrochemical characterization is performed by constant capacitance (ConCap) measurements, and the film morphology is characterized by atomic force microscopy (AFM) and scanning electron microscopy (SEM). An increase in the average sensitivity of the modified biosensors (EIS–nanofilm–enzyme) of around 15% is found in relation to sensors, only carrying the enzyme but without the nanofilm (EIS–enzyme). In this sense, the nanofilm acts as a stable bioreceptor onto the EIS chip improving the output signal in terms of sensitivity and stability.
KW  - capacitive electrolyte–insulator–semiconductor sensors
KW  - graphene oxide
KW  - layer-by-layer technique
KW  - nanomaterials
KW  - polyaniline
Y1  - 2021
U6  - http://dx.doi.org/10.1002/pssa.202000747
SN  - 1862-6319
N1  - Corresponding author: José R. Siqueira Jr & Michael J. Schöning
VL  - 218
IS  - 13
SP  - 1
EP  - 9
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Paczkowski, Sebastian
A1  - Weißbecker, Bernhard
A1  - Schöning, Michael Josef
A1  - Schütz, Stefan
T1  - Biosensors on the Basis of Insect Olfaction
JF  - Insect biotechnology / Andreas Vilcinskas, ed.
Y1  - 2011
SN  - 978-90-481-9640-1
N1  - Biologically-inspired system ; 2
SP  - 225
EP  - 240
PB  - Springer
CY  - Dordrecht [u.a.]
ER  - 
TY  - JOUR
A1  - Pilas, Johanna
A1  - Iken, Heiko
A1  - Selmer, Thorsten
A1  - Keusgen, Michael
A1  - Schöning, Michael Josef
T1  - Development of a multi‐parameter sensor chip for the simultaneous detection of organic compounds in biogas processes
JF  - Physica status solidi (a)
N2  - An enzyme-based multi-parameter biosensor is developed for monitoring the concentration of formate, d-lactate, and l-lactate in biological samples. The sensor is based on the specific dehydrogenation by an oxidized β-nicotinamide adenine dinucleotide (NAD+)-dependent dehydrogenase (formate dehydrogenase, d-lactic dehydrogenase, and l-lactic dehydrogenase, respectively) in combination with a diaphorase from Clostridium kluyveri (EC 1.8.1.4). The enzymes are immobilized on a platinum working electrode by cross-linking with glutaraldehyde (GA). The principle of the determination scheme in case of l-lactate is as follows: l-lactic dehydrogenase (l-LDH) converts l-lactate into pyruvate by reaction with NAD+. In the presence of hexacyanoferrate(III), the resulting reduced β-nicotinamide adenine dinucleotide (NADH) is then regenerated enzymatically by diaphorase. The electrochemical detection is based on the current generated by oxidation of hexacyanoferrate(II) at an applied potential of +0.3 V vs. an Ag/AgCl reference electrode. The biosensor will be electrochemically characterized in terms of linear working range and sensitivity. Additionally, the successful practical application of the sensor is demonstrated in an extract from maize silage.
Y1  - 2015
U6  - http://dx.doi.org/10.1002/pssa.201431894
SN  - 1862-6319
VL  - 212
IS  - 6
SP  - 1306
EP  - 1312
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Pilas, Johanna
A1  - Mariano, K.
A1  - Keusgen, M.
A1  - Selmer, Thorsten
A1  - Schöning, Michael Josef
T1  - Optimization of an Enzyme-based Multi-parameter Biosensor for Monitoring Biogas Processes
JF  - Procedia Engineering
Y1  - 2015
U6  - http://dx.doi.org/10.1016/j.proeng.2015.08.702
SN  - 1877-7058
N1  - Part of special issue "Eurosensors 2015"
VL  - 120
SP  - 532
EP  - 535
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Pilas, Johanna
A1  - Selmer, Thorsten
A1  - Keusgen, Michael
A1  - Schöning, Michael Josef
T1  - Screen-printed carbon electrodes modified with graphene oxide for the design of a reagent-free NAD+-dependent biosensor array
JF  - Analytical Chemistry
Y1  - 2019
U6  - http://dx.doi.org/10.1021/acs.analchem.9b04481
VL  - 91
IS  - 23
SP  - 15293
EP  - 15299
PB  - ACS Publications
CY  - Washington
ER  - 
TY  - JOUR
A1  - Pilas, Johanna
A1  - Yazici, Y.
A1  - Selmer, Thorsten
A1  - Keusgen, M.
A1  - Schöning, Michael Josef
T1  - Application of a portable multi-analyte biosensor for organic acid determination in silage
JF  - Sensors
N2  - Multi-analyte biosensors may offer the opportunity to perform cost-effective and rapid analysis with reduced sample volume, as compared to electrochemical biosensing of each analyte individually. This work describes the development of an enzyme-based biosensor system for multi-parametric determination of four different organic acids. The biosensor array comprises five working electrodes for simultaneous sensing of ethanol, formate, d-lactate, and l-lactate, and an integrated counter electrode. Storage stability of the biosensor was evaluated under different conditions (stored at +4 °C in buffer solution and dry at −21 °C, +4 °C, and room temperature) over a period of 140 days. After repeated and regular application, the individual sensing electrodes exhibited the best stability when stored at −21 °C. Furthermore, measurements in silage samples (maize and sugarcane silage) were conducted with the portable biosensor system. Comparison with a conventional photometric technique demonstrated successful employment for rapid monitoring of complex media.
Y1  - 2018
U6  - http://dx.doi.org/10.3390/s18051470
SN  - 1424-8220
VL  - 18
IS  - 5
PB  - MDPI
CY  - Basel
ER  -