TY  - JOUR
A1  - Guo, Yuanyuan
A1  - Miyamoto, Ko-ichiro
A1  - Wagner, Torsten
A1  - Schöning, Michael Josef
A1  - Yoshinobu, Tatsuo
T1  - Theoretical study and simulation of light-addressable potentiometric sensors
JF  - Physica status solidi (A) : applications and materials
N2  - The light-addressable potentiometric sensor (LAPS) is a semiconductor-based potentiometric sensor using a light probe with an ability of detecting the concentration of biochemical species in a spatially resolved manner. As an important biomedical sensor, research has been conducted to improve its performance, for instance, to realize high-speed measurement. In this work, the idea of facilitating the device-level simulation, instead of using an equivalent-circuit model, is presented for detailed analysis and optimization of the performance of the LAPS. Both carrier distribution and photocurrent response have been simulated to provide new insight into both amplitude-mode and phase-mode operations of the LAPS. Various device parameters can be examined to effectively design and optimize the LAPS structures and setups for enhanced performance.
Y1  - 2014
U6  - https://doi.org/10.1002/pssa.201330354
SN  - 0031-8965
VL  - 211
IS  - 6
SP  - 1467
EP  - 1472
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Goetze, Sandra
A1  - Baer, Alexandra
A1  - Winkelmann, Silke
A1  - Nehlsen, Kristina
A1  - Seibler, Jost
A1  - Maass, Karin
A1  - Bode, Jürgen
T1  - Performance of genomic bordering elements at predefined genomic loci
JF  - Molecular and Cellular Biology
Y1  - 2005
U6  - https://doi.org/10.1128/MCB.25.6.2260-2272.2005
SN  - 1098-5549
VL  - 25
IS  - 6
SP  - 2260
EP  - 2272
ER  - 
TY  - JOUR
A1  - Glaser, Stefan
A1  - Lubitz, Sandra
A1  - Loveland, Kate L.
A1  - Ohbo, Kazu
A1  - Robb, Lorraine
A1  - Schwenk, Frieder
A1  - Seibler, Jost
A1  - Roellig, Daniela
A1  - Kranz, Andrea
A1  - Anastassiadis, Konstantinos
A1  - Stewart, A. Francis
T1  - The histone 3 lysine 4 methyltransferase, Mll2, is only required briefly in development and spermatogenesis
JF  - Epigenetics & Chromatin
Y1  - 2009
U6  - https://doi.org/10.1186/1756-8935-2-5
SN  - 1756-8935
VL  - 2
IS  - 5
SP  - 1
EP  - 16
ER  - 
TY  - JOUR
A1  - Gielen, Hans-Günther
A1  - Wolf, Günter
A1  - Berndt, Heinz
A1  - Zahn, Helmut
T1  - Synthese der Fragmente A1–8, A9–15 und A16–21 der Schafinsulin-A-Kette unter Verwendung des S-tert-Butylmercaptorestes als Thiolschutzgruppe
JF  - Hoppe-Seyler's Zeitschrift für physiologische Chemie
Y1  - 1979
U6  - https://doi.org/10.1515/bchm2.1979.360.2.1535
SN  - 1437-4315
SN  - 0018-4888
VL  - 360
IS  - 2
SP  - 1535
EP  - 1548
ER  - 
TY  - JOUR
A1  - Ghosch, S.
A1  - Baier, M.
A1  - Schütz, J.
A1  - Schneider, Felix
A1  - Scherer, Ulrich W.
T1  - Analysis of electronic autoradiographs by mathematical post-processing
JF  - Radiation Effects and Defects in Solids: Incorporating plasma science and plasma technology
N2  - Autoradiography is a well-established method of nuclear imaging. When different radionuclides are present simultaneously, additional processing is needed to distinguish distributions of radionuclides. In this work, a method is presented where aluminium absorbers of different thickness are used to produce images with different cut-off energies. By subtracting images pixel-by-pixel one can generate images representing certain ranges of β-particle energies. The method is applied to the measurement of irradiated reactor graphite samples containing several radionuclides to determine the spatial distribution of these radionuclides within pre-defined energy windows. The process was repeated under fixed parameters after thermal treatment of the samples. The greyscale images of the distribution after treatment were subtracted from the corresponding pre-treatment images. Significant changes in the intensity and distribution of radionuclides could be observed in some samples. Due to the thermal treatment parameters the most significant differences were observed in the ³H and ¹⁴C inventory and distribution.
Y1  - 2016
U6  - https://doi.org/10.1080/10420150.2016.1155587
SN  - 1029-4953
VL  - 171
IS  - 1-2
SP  - 161
EP  - 172
PB  - Taylor & Francis
CY  - London
ER  - 
TY  - JOUR
A1  - Gerigk, M.
A1  - Maaß, D.
A1  - Takors, Ralf
A1  - Kreutzer, A.
A1  - Wandrey, Christian
A1  - Bongaerts, Johannes
A1  - Wubbolts, Marcel
T1  - Fermentative Herstellung von L-Phenylalanin im Fed-Batch Verfahren mit E. coli unter Einbindung eines integrierten Aufarbeitungsverfahrens
JF  - Chemie-Ingenieur-Technik (CIT)
Y1  - 2000
SN  - 1522-2640 (E-Journal); 0009-286X (Print)
N1  - Printausg. in der Bibliothek vorhanden: 63 ZS 022
VL  - Vol. 72
IS  - Iss. 9
SP  - 926
EP  - 927
ER  - 
TY  - JOUR
A1  - Gerigk, M.
A1  - Maaß, D.
A1  - Kreutzer, A.
A1  - Sprenger, G.
A1  - Bongaerts, Johannes
A1  - Wubbolts, Marcel
A1  - Takors, Ralf
T1  - Enhanced pilot-scale fed-batch L-phenylalanine production with recombinant Escherichia coli by fully integrated reactive extraction
JF  - Bioprocess and biosystems engineering
Y1  - 2002
SN  - 1432-0797 (E-Journal); 1615-7605 (E-Journal); 0178-515X (Print); 1615-7591 (Print)
VL  - Vol. 25
IS  - Iss. 1
SP  - 43
EP  - 52
ER  - 
TY  - JOUR
A1  - Gerigk, M.
A1  - Bujnicki, R.
A1  - Ganpo-Nkwenkwa, E.
A1  - Bongaerts, Johannes
A1  - Sprenger, G.
A1  - Takors, Ralf
T1  - Process control for enhanced L-phenylalanine production using different recombinant Escherichia coli strains
JF  - Biotechnology and bioengineering
Y1  - 2002
SN  - 1097-0290 (E-Journal); 0006-3592 (Print)
VL  - Vol. 80
IS  - Iss. 7
SP  - 746
EP  - 754
ER  - 
TY  - JOUR
A1  - Gebeshuber, Christoph A.
A1  - Kornauth, Christoph
A1  - Dong, Lihua
A1  - Sierig, Ralph
A1  - Seibler, Jost
A1  - Reiss, Martina
A1  - Tauber, Stefanie
A1  - Bilban, Martin
A1  - Wang, Shijun
A1  - Kain, Renate
A1  - Böhmig, Georg A.
A1  - Moeller, Marcus J.
A1  - Gröne, Hermann-Josef
A1  - Englert, Christoph
A1  - Martinez, Javier
A1  - Kerjaschki, Dontscho
T1  - Focal segmental glomerulosclerosis is induced by microRNA-193a and its downregulation of WT1
JF  - Nature Medicine
Y1  - 2013
U6  - https://doi.org/10.1038/nm.3142
SN  - 1078-8956
VL  - 19
IS  - 4
SP  - 481
EP  - 487
ER  - 
TY  - JOUR
A1  - Feuerriegel, Uwe
A1  - Stahlberg, R.
A1  - Weisenburger, P.
A1  - Steiger, F.
T1  - Massen- und Energiebilanzen unter Einbeziehung der Untersuchungen an der Thermoselect-Anlage Fondotoce (Italien). Stahlberg, R.; Feuerriegel, U.; Weisenburger, P.; Steiger, F.
JF  - Thermoselect : der neue Weg, Restmüll umweltgerecht zu behandeln ; [Tagungsband zur Fachtagung Thermoselect am 19. Januar 1995 in Rastatt/Baden] / Günther Häßler. 2. durchges. und erw. Aufl.
Y1  - 1995
SN  - 3-9803665-4-5
N1  - Fachtagung THERMOSELECT ; (1995, Rastatt) ; Fachtagung Thermoselect ; (1995.01.19 : ; Rastatt)
SP  - 55
EP  - 84
PB  - Goerner
CY  - Karlsruhe
ER  - 
TY  - JOUR
A1  - Feuerriegel, Uwe
A1  - Stahlberg, R.
A1  - Riegel, J.
A1  - Steiger, F.
T1  - Thermoselect – Energie- und Rohstoffgewinnung: Teil 3. Massen- und Energiebilanz unter Berücksichtigung der Wirtschaftlichkeit der Synthesegasnutzung. Stahlberg, R.; Riegel, J.; Feuerriegel, U.; Steiger, F.
JF  - Chemische Technik <Leipzig>. 47 (1995), H. 3
Y1  - 1995
SN  - 0045-6519
SP  - 113
EP  - 121
ER  - 
TY  - JOUR
A1  - Feuerriegel, Uwe
A1  - Stahlberg, R.
T1  - Performance of the Thermoselect Demonstration Plant at Fondotoce, Italy: Product Quality and Distribution of Chemical Elements in the Material Flow. Stahlberg, R. ; Feuerriegel, U.
JF  - Solid waste management: thermal treatment & waste-to-energy technologies : proceedings of an international specialty conference sponsored by the Air & Waste Management Association, [Washington, DC, April 18 - 21, 1995] / comp. by James D. Kilgroe
Y1  - 1996
N1  - International Conference on Solid Waste Management, Thermal Treatment and Waste to Energy ; <1995, Washington, DC>
PB  - Air & Waste Management Assoc.
CY  - Pittsburgh, Pa.
ER  - 
TY  - JOUR
A1  - Feuerriegel, Uwe
A1  - Stahlberg, R.
T1  - Thermoselect – Energie- und Rohstoffgewinnung: Teil 1. Verfahrensgrundlagen zur unterbrechungslosen Verwertung von Restabfällen. Stahlberg, R. ; Feuerriegel, U.
JF  - Chemische Technik <Leipzig>. 46 (1994), H. 5
Y1  - 1994
SN  - 0045-6519
SP  - 257
EP  - 266
ER  - 
TY  - JOUR
A1  - Feuerriegel, Uwe
A1  - Stahlberg, R.
T1  - Thermoselect – Energie- und Rohstoffgewinnung: Teil 2. Untersuchungen an der Thermoselect-Anlage Fondotoce (Italien), Qualitäten der Produkte und Nachweis der Verteilung der chemischen Elemente in den Stoffströmen. Stahlberg, R. ; Feuerriegel, U.
JF  - Chemische Technik <Leipzig>. 47 (1995), H. 1
Y1  - 1995
SN  - 0045-6519
SP  - 1
EP  - 10
ER  - 
TY  - JOUR
A1  - Feuerriegel, Uwe
A1  - Stahlberg, R.
T1  - Das Thermoselect-Verfahren zur Energie- und Rohstoffgewinnung – Konzepte, Verfahren, Kosten
JF  - Thermische Abfallentsorgung – Konzepte, Kosten, Verfahren. Tagung Veitshöchheim, 27./28.06.1995, VDI-Gesellschaft Energietechnik
Y1  - 1995
SN  - 0083-5560
N1  - VDI-Bericht 1192
SP  - 319
EP  - 319
PB  - VDI-Verl.
CY  - Düsseldorf
ER  - 
TY  - JOUR
A1  - Feuerriegel, Uwe
A1  - Stahlberg, R.
T1  - Thermoselect – Energie- und Rohstoffgewinnung, Verfahrensgrundlagen zur unterbrechungslosen Verwertung von Restabfällen. Stahlberg, R.; Feuerriegel, U.
JF  - Thermoselect : der neue Weg, Restmüll umweltgerecht zu behandeln ; [Tagungsband zur Fachtagung Thermoselect am 19. Januar 1995 in Rastatt/Baden] / Günther Häßler. 2. durchges. und erw. Aufl.
Y1  - 1995
SN  - 3-9803665-4-5
N1  - Fachtagung THERMOSELECT ; (1995, Rastatt) ; Fachtagung Thermoselect ; (1995.01.19 : ; Rastatt)
SP  - 13
EP  - 32
PB  - Goerner
CY  - Karlsruhe
ER  - 
TY  - JOUR
A1  - Feuerriegel, Uwe
A1  - Stahlberg, R.
T1  - Untersuchungen an der Thermoselect-Anlage Fondotoce (Italien), Qualitäten der Produkte und Nachweis der Verteilung der chemischen Elemente in den Stoffströmen. Stahlberg, R.; Feuerriegel, U.
JF  - Thermoselect : der neue Weg, Restmüll umweltgerecht zu behandeln ; [Tagungsband zur Fachtagung Thermoselect am 19. Januar 1995 in Rastatt/Baden] / Günther Häßler. 2. durchges. und erw. Aufl.
Y1  - 1995
SN  - 3-9803665-4-5
N1  - Fachtagung THERMOSELECT ; (1995, Rastatt) ; Fachtagung Thermoselect ; (1995.01.19 : ; Rastatt)
SP  - 33
EP  - 54
PB  - Goerner
CY  - Karlsruhe
ER  - 
TY  - JOUR
A1  - Feuerriegel, Uwe
A1  - Stahlberg, R.
T1  - Thermoselect – Energie- und Rohstoffgewinnung. Stahlberg, R. ; Feuerriegel, U.
JF  - Abfallwirtschaft Stoffkreisläufe / Terratec '95, Kongress West-Ost-Transfer Umwelt vom 1. bis 3. März 1995. Leipziger Messe. Hrsg. von Hasso Scheffler ...
Y1  - 1995
SN  - 3-8154-3513-7
N1  - Terratec <1995, Leipzig> ; Kongress West-Ost-Transfer Umwelt <1995, Leipzig> ; Leipziger Messe
SP  - 179
EP  - 194
PB  - Teubner
CY  - Stuttgart [u.a.]
ER  - 
TY  - JOUR
A1  - Feuerriegel, Uwe
A1  - Ma, J.
A1  - Hoffmann, U.
T1  - Untersuchungen zur Rußfilterregeneration mit Hilfe von Zündhilfen. Ma, J.; Hoffmann, U.; Feuerriegel, U
JF  - Erdöl und Kohle – Erdgas, Brennstoff-Chemie. 43 (1990)
Y1  - 1990
SN  - 0014-0058
N1  - Druckausgabe in Jülich unter 63 ZS 034-1990 vorhanden
SP  - 150
EP  - 157
ER  - 
TY  - JOUR
A1  - Feuerriegel, Uwe
A1  - Künsch, M.
A1  - Stahlberg, R.
A1  - Steiger, F.
T1  - Massen- und Energiebilanz des Thermoselect-Verfahrens
JF  - Thermoselect-Verfahren zur Ent- und Vergasung von Abfällen / Franz J. Schweitzer [Hrsg.]
Y1  - 1994
SN  - 3-924511-47-0
PB  - EF-Verl. für Energie- u. Umwelttechnik
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Feuerriegel, Uwe
A1  - Klose, W.
A1  - Sloboshanin, S.
A1  - Goebel, H. [u.a.]
T1  - Deactivation of a palladium-supported alumina catalyst by hydrogen sulfide during the oxidation of methane
JF  - Langmuir: the ACS journal of surfaces and colloids. 10 (1994), H. 10
Y1  - 1994
SN  - 0743-74363
SP  - 3567
EP  - 3570
ER  - 
TY  - JOUR
A1  - Feuerriegel, Uwe
A1  - Klose, W.
T1  - Reaktionskinetik der katalytischen Methan-Oxidation und experimentelle Untersuchungen zur Desaktivierung eines Palladium-Trägerkatalysators durch Schwefelwasserstoff. Klose, W. ; Feuerriegel, U.
JF  - Chemie - Ingenieur - Technik. 65 (1993), H. 10
Y1  - 1993
SN  - 0009-286X
N1  - engl. Titel: Reaction Kinetics of Catalytic Methane Oxidation and Deactivation of a Palladium Catalyst by Hydrogen Sulfide During Catalytic Oxidation of Methane
SP  - 1242
EP  - 1243
ER  - 
TY  - JOUR
A1  - Feuerriegel, Uwe
A1  - Klose, W.
T1  - Untersuchungen zum Mechanismus der Desaktivierung eines Palladium-Trägerkatalysators durch Vergiftung mit Schwefelwasserstoff
JF  - Chemie - Ingenieur - Technik. 70 (1998), H. 4
Y1  - 1998
SN  - 0009-286X
N1  - Artikel in Englisch unter <a href="http://www3.interscience.wiley.com/cgi-bin/fulltext/40001930/PDFSTART">Chemical engineering & technology. 21(1998)7. S. 562-564</a>
SP  - 449
EP  - 452
ER  - 
TY  - JOUR
A1  - Feuerriegel, Uwe
A1  - Klose, W.
T1  - Investigations into the Mechanism of the Deactivation of a Palladium-Supported Catalyst by Hydrogen Sulfide Poisoning. Klose, W. ; Feuerriegel, U.
JF  - Chemical engineering & technology. 21 (1998), H. 7
Y1  - 1998
SN  - 0930-7516
N1  - gleicher Artikel in Deutsch In: Chem.Ing.Tech 70(1998) No  S. 449-452 u.d.T.: Untersuchungen zum Mechanismus der Desaktivierung eines Palladium-Trägerkatalysators durch Vergiftung mit Schwefelwasserstoff
SP  - 562
EP  - 564
ER  - 
TY  - JOUR
A1  - Feuerriegel, Uwe
A1  - Hoffmann, Ulrich
A1  - Walenzik, C.
A1  - Kögel, J.
T1  - Für Scale-up´s und Betriebsmuster, Projekt Trocknungstechnik an der FH Aachen
JF  - CITplus : das Praxismagazin für Verfahrenstechnik und Apparatebau. 4 (2001)
Y1  - 2001
SN  - 1436-2597
SP  - 24
EP  - 25
ER  - 
TY  - JOUR
A1  - Feuerriegel, Uwe
T1  - Energetische Verwertung von Kunststoffabfällen
JF  - Chemie und Technologie makromolekularer Stoffe :, 16. Kolloquium.  / Fachhochschule <Aachen> / Fachbereich Chemieingenieurwesen. 20. November 1998 an der FH Aachen, Fachbereich Chemieingenieurwesen
Y1  - 1998
N1  - FH-Texte. 69; In Jülich unter der Signatur: 61 ZOW 405-16
SP  - 169
EP  - 189
PB  - Fachhochschule Aachen
CY  - Aachen
ER  - 
TY  - JOUR
A1  - Feuerriegel, Uwe
T1  - Trennen und Trocknen. FH Aachen verfügt über einen Zentrifugentrockner im Technikumsmaßstab
JF  - Lebensmitteltechnik. 33 (2001), H. 7-8
Y1  - 2001
SN  - 0047-4290
SP  - 22
EP  - 23
ER  - 
TY  - JOUR
A1  - Feng, Yong-Qing
A1  - Seibler, Jost
A1  - Alami, Raouf
A1  - Eisen, Andrew
A1  - Westerman, Karen A.
A1  - Leboulch, Philippe
A1  - Fiering, Steven
A1  - Bouhassira, Eric E.
T1  - Site-specific chromosomal integration in mammalian cells: highly efficient CRE recombinase-mediated cassette exchange
JF  - Journal of Molecular Biology
Y1  - 1999
U6  - https://doi.org/10.1006/jmbi.1999.3113
SN  - 0022-2836
VL  - 292
IS  - 4
SP  - 779
EP  - 785
ER  - 
TY  - JOUR
A1  - Falkenberg, Fabian
A1  - Voß, Leonie
A1  - Bott, Michael
A1  - Bongaerts, Johannes
A1  - Siegert, Petra
T1  - New robust subtilisins from halotolerant and halophilic Bacillaceae
JF  - Applied Microbiology and Biotechnology
N2  - The aim of the present study was the characterisation of three true subtilisins and one phylogenetically intermediate subtilisin from halotolerant and halophilic microorganisms. Considering the currently growing enzyme market for efficient and novel biocatalysts, data mining is a promising source for novel, as yet uncharacterised enzymes, especially from halophilic or halotolerant Bacillaceae, which offer great potential to meet industrial needs. Both halophilic bacteria Pontibacillus marinus DSM 16465ᵀ and Alkalibacillus haloalkaliphilus DSM 5271ᵀ and both halotolerant bacteria Metabacillus indicus DSM 16189 and Litchfieldia alkalitelluris DSM 16976ᵀ served as a source for the four new subtilisins SPPM, SPAH, SPMI and SPLA. The protease genes were cloned and expressed in Bacillus subtilis DB104. Purification to apparent homogeneity was achieved by ethanol precipitation, desalting and ion-exchange chromatography. Enzyme activity could be observed between pH 5.0–12.0 with an optimum for SPPM, SPMI and SPLA around pH 9.0 and for SPAH at pH 10.0. The optimal temperature for SPMI and SPLA was 70 °C and for SPPM and SPAH 55 °C and 50 °C, respectively. All proteases showed high stability towards 5% (w/v) SDS and were active even at NaCl concentrations of 5 M. The four proteases demonstrate potential for future biotechnological applications.
KW  - Biotechnological application
KW  - Bacillaceae
KW  - Subtilisin
KW  - Subtilases
KW  - Halotolerant protease
Y1  - 2023
U6  - https://doi.org/10.1007/s00253-023-12553-w
SN  - 1432-0614
N1  - Corresponding author: Petra Siegert
VL  - 107
SP  - 3939
EP  - 3954
PB  - Springer Nature
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Falkenberg, Fabian
A1  - Rahba, Jade
A1  - Fischer, David
A1  - Bott, Michael
A1  - Bongaerts, Johannes
A1  - Siegert, Petra
T1  - Biochemical characterization of a novel oxidatively stable, halotolerant, and high-alkaline subtilisin from Alkalihalobacillus okhensis Kh10-101T
JF  - FEBS Open Bio
N2  - Halophilic and halotolerant microorganisms represent a promising source of salt-tolerant enzymes suitable for various biotechnological applications where high salt concentrations would otherwise limit enzymatic activity. Considering the current growing enzyme market and the need for more efficient and new biocatalysts, the present study aimed at the characterization of a high-alkaline subtilisin from Alkalihalobacillus okhensis Kh10-101T. The protease gene was cloned and expressed in Bacillus subtilis DB104. The recombinant protease SPAO with 269 amino acids belongs to the subfamily of high-alkaline subtilisins. The biochemical characteristics of purified SPAO were analyzed in comparison with subtilisin Carlsberg, Savinase, and BPN'. SPAO, a monomer with a molecular mass of 27.1 kDa, was active over a wide range of pH 6.0–12.0 and temperature 20–80 °C, optimally at pH 9.0–9.5 and 55 °C. The protease is highly oxidatively stable to hydrogen peroxide and retained 58% of residual activity when incubated at 10 °C with 5% (v/v) H2O2 for 1 h while stimulated at 1% (v/v) H2O2. Furthermore, SPAO was very stable and active at NaCl concentrations up to 5.0 m. This study demonstrates the potential of SPAO for biotechnological applications in the future.
KW  - Alkalihalobacillus okhensis
KW  - detergent protease
KW  - halotolerant protease
KW  - high-alkaline subtilisin
KW  - oxidative stable protease
Y1  - 2022
U6  - https://doi.org/10.1002/2211-5463.13457
SN  - 2211-5463
N1  - Corresponding author: Petra Siegert
VL  - 12
IS  - 10
SP  - 1729
EP  - 1746
PB  - Wiley
CY  - Hoboken, NJ
ER  - 
TY  - JOUR
A1  - Falkenberg, Fabian
A1  - Kohn, Sophie
A1  - Bott, Michael
A1  - Bongaerts, Johannes
A1  - Siegert, Petra
T1  - Biochemical characterisation of a novel broad pH spectrum subtilisin from Fictibacillus arsenicus DSM 15822ᵀ
JF  - FEBS Open Bio
N2  - Subtilisins from microbial sources, especially from the Bacillaceae family, are of particular interest for biotechnological applications and serve the currently growing enzyme market as efficient and novel biocatalysts. Biotechnological applications include use in detergents, cosmetics, leather processing, wastewater treatment and pharmaceuticals. To identify a possible candidate for the enzyme market, here we cloned the gene of the subtilisin SPFA from Fictibacillus arsenicus DSM 15822ᵀ (obtained through a data mining-based search) and expressed it in Bacillus subtilis DB104. After production and purification, the protease showed a molecular mass of 27.57 kDa and a pI of 5.8. SPFA displayed hydrolytic activity at a temperature optimum of 80 °C and a very broad pH optimum between 8.5 and 11.5, with high activity up to pH 12.5. SPFA displayed no NaCl dependence but a high NaCl tolerance, with decreasing activity up to concentrations of 5 m NaCl. The stability enhanced with increasing NaCl concentration. Based on its substrate preference for 10 synthetic peptide 4-nitroanilide substrates with three or four amino acids and its phylogenetic classification, SPFA can be assigned to the subgroup of true subtilisins. Moreover, SPFA exhibited high tolerance to 5% (w/v) SDS and 5% H₂O₂ (v/v). The biochemical properties of SPFA, especially its tolerance of remarkably high pH, SDS and H₂O₂, suggest it has potential for biotechnological applications.
KW  - Bacillaceae
KW  - Biotechnological application
KW  - Broad pH spectrum
KW  - Subtilases
KW  - Subtilisin
Y1  - 2023
U6  - https://doi.org/10.1002/2211-5463.13701
SN  - 2211-5463
N1  - Corresponding author: Petra Siegert
VL  - 13
IS  - 11
SP  - 2035
EP  - 2046
PB  - Wiley
CY  - Hoboken, NJ
ER  - 
TY  - JOUR
A1  - Falkenberg, Fabian
A1  - Bott, Michael
A1  - Bongaerts, Johannes
A1  - Siegert, Petra
T1  - Phylogenetic survey of the subtilase family and a data-mining-based search for new subtilisins from Bacillaceae
JF  - Frontiers in Microbiology
N2  - The subtilase family (S8), a member of the clan SB of serine proteases are ubiquitous in all kingdoms of life and fulfil different physiological functions. Subtilases are divided in several groups and especially subtilisins are of interest as they are used in various industrial sectors. Therefore, we searched for new subtilisin sequences of the family Bacillaceae using a data mining approach. The obtained 1,400 sequences were phylogenetically classified in the context of the subtilase family. This required an updated comprehensive overview of the different groups within this family. To fill this gap, we conducted a phylogenetic survey of the S8 family with characterised holotypes derived from the MEROPS database. The analysis revealed the presence of eight previously uncharacterised groups and 13 subgroups within the S8 family. The sequences that emerged from the data mining with the set filter parameters were mainly assigned to the subtilisin subgroups of true subtilisins, high-alkaline subtilisins, and phylogenetically intermediate subtilisins and represent an excellent source for new subtilisin candidates.
Y1  - 2022
U6  - https://doi.org/10.3389/fmicb.2022.1017978
SN  - 1664-302X
VL  - 2022
IS  - 13
PB  - Frontiers
CY  - Lausanne
ER  - 
TY  - JOUR
A1  - Everaers, Ralf
A1  - Karimi-Varzaneh, Hossein Ali
A1  - Fleck, Franz
A1  - Hojdis, Nils
A1  - Svaneborg, Carsten
T1  - Kremer–Grest Models for Commodity Polymer Melts: Linking Theory, Experiment, and Simulation at the Kuhn Scale
JF  - Macromolecules
N2  - The Kremer–Grest (KG) polymer model is a standard model for studying generic polymer properties in molecular dynamics simulations. It owes its popularity to its simplicity and computational efficiency, rather than its ability to represent specific polymers species and conditions. Here we show that by tuning the chain stiffness it is possible to adapt the KG model to model melts of real polymers. In particular, we provide mapping relations from KG to SI units for a wide range of commodity polymers. The connection between the experimental and the KG melts is made at the Kuhn scale, i.e., at the crossover from the chemistry-specific small scale to the universal large scale behavior. We expect Kuhn scale-mapped KG models to faithfully represent universal properties dominated by the large scale conformational statistics and dynamics of flexible polymers. In particular, we observe very good agreement between entanglement moduli of our KG models and the experimental moduli of the target polymers.
Y1  - 2020
U6  - https://doi.org/10.1021/acs.macromol.9b02428
SN  - 1520-5835
VL  - 53
IS  - 6
SP  - 1901
EP  - 1916
PB  - ACS Publications
CY  - Washington, DC
ER  - 
TY  - JOUR
A1  - Engel, Mareike
A1  - Holtmann, Dirk
A1  - Ulber, Roland
A1  - Tippkötter, Nils
T1  - Increased Biobutanol Production by Mediator‐Less Electro‐Fermentation
JF  - Biotechnology Journal
N2  - A future bio-economy should not only be based on renewable raw materials but also in the raise of carbon yields of existing production routes. Microbial electrochemical technologies are gaining increased attention for this purpose. In this study, the electro-fermentative production of biobutanol with C. acetobutylicum without the use of exogenous mediators is investigated regarding the medium composition and the reactor design. It is shown that the use of an optimized synthetic culture medium allows higher product concentrations, increased biofilm formation, and higher conductivities compared to a synthetic medium supplemented with yeast extract. Moreover, the optimization of the reactor system results in a doubling of the maximum product concentrations for fermentation products. When a working electrode is polarized at −600 mV vs. Ag/AgCl, a shift from butyrate to acetone and butanol production is induced. This leads to an increased final solvent yield of Yᴀᴃᴇ = 0.202 gg⁻¹ (control 0.103 gg⁻¹), which is also reflected in a higher carbon efficiency of 37.6% compared to 23.3% (control) as well as a fourfold decrease in simplified E-factor to 0.43. The results are promising for further development of biobutanol production in bioelectrochemical systems in order to fulfil the principles of Green Chemistry.
Y1  - 2018
U6  - https://doi.org/10.1002/biot.201800514
SN  - 1860-7314
VL  - 14
IS  - 4
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Engel, Mareike
A1  - Gemünde, Andre
A1  - Holtmann, Dirk
A1  - Müller-Renno, Christine
A1  - Ziegler, Christiane
A1  - Tippkötter, Nils
A1  - Ulber, Roland
T1  - Clostridium acetobutylicum’s connecting world: cell appendage formation in bioelectrochemical systems
JF  - ChemElectroChem
N2  - Bacterial cell appendix formation supports cell-cell interaction, cell adhesion and cell movement. Additionally, in bioelectrochemical systems (BES), cell appendages have been shown to participate in extracellular electron transfer. In this work, the cell appendix formation of Clostridium acetobutylicum in biofilms of a BES are imaged and compared with conventional biofilms. Under all observed conditions, the cells possess filamentous appendages with a higher number and density in the BES. Differences in the amount of extracellular polymeric substance in the biofilms of the electrodes lead to the conclusion that the cathode can be used as electron donor and the anode as electron acceptor by C. acetobutylicum. When using conductive atomic force microscopy, a current response of about 15 nA is found for the cell appendages from the BES. This is the first report of conductivity for clostridial cell appendices and represents the basis for further studies on their role for biofilm formation and electron transfer.
Y1  - 2019
U6  - https://doi.org/10.1002/celc.201901656
SN  - 2196-0216
VL  - 7
IS  - 2
SP  - 414
EP  - 420
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Engel, Mareike
A1  - Bayer, Hendrik
A1  - Holtmann, Dirk
A1  - Tippkötter, Nils
A1  - Ulber, Roland
T1  - Flavin secretion of Clostridium acetobutylicum in a bioelectrochemical system - Is an iron limitation involved?
JF  - Bioelectrochemistry
Y1  - 2019
U6  - https://doi.org/10.1016/j.bioelechem.2019.05.014
SN  - 1567-5394
IS  - In Press, Accepted Manuscript
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Elbers, Gereon
A1  - Zang, Thorsten
A1  - Buck, Manfred
T1  - Ruß-Immissionsmessungen im Einflußbereich des Kraftfahrzeugverkehrs
JF  - Staub, Reinhaltung der Luft : air quality control. 50 (1990)
Y1  - 1990
SN  - 0039-0771
SP  - 93
ER  - 
TY  - JOUR
A1  - Elbers, Gereon
A1  - Thomzik, Manfred
T1  - Das Entstehen geruchsintensiver schwefelhaltiger organischer Verbindungen in Lackierereien und Möglichkeiten zu deren Vermeidung
JF  - Aus der Tätigkeit der LIS / Landesanstalt für Immissionsschutz des Landes Nordrhein-Westfalen. 1989 (1990)
Y1  - 1990
SN  - 0931-5497
SP  - 35
ER  - 
TY  - JOUR
A1  - Elbers, Gereon
A1  - Richter, J.
T1  - Messung kraftfahrzeugbedingter Rußimmissionen
JF  - Staub, Reinhaltung der Luft : air quality control. 54 (1994)
Y1  - 1994
SN  - 0039-0771
SP  - 19
ER  - 
TY  - JOUR
A1  - Elbers, Gereon
A1  - Remme, S.
A1  - Lehmann, G.
T1  - EPR of Cr3+ in Tris(acetylacetonato)gallium(III) Single Crystals
JF  - Inorganic Chemistry. 25 (1986)
Y1  - 1986
SN  - 0020-1669
SP  - 896
EP  - 897
ER  - 
TY  - JOUR
A1  - Elbers, Gereon
A1  - Remme, S.
A1  - Lehmann, G.
T1  - EPR and Optical Absorption of Cr3+ in CsCl and CsBr
JF  - Physica Status Solidi (B). 142 (1987), H. 2
Y1  - 1987
SN  - 0031-8957
SP  - 367
EP  - 377
ER  - 
TY  - JOUR
A1  - Elbers, Gereon
A1  - Pfeffer, Hans-Ulrich
A1  - Friesel, Jürgen
A1  - Beier, Reinhold
T1  - Air Pollution Monitoring in Street Canyons / Hans-Ulrich Pfeffer, Jürgen Friesel, Gereon Elbers, Reinhold Beier,
JF  - Transport and air pollution : proceedings ; 3rd international symposium, Palais des Papes, Avignon, France, 6 - 10 June 1994 = Transports et pollution de l'air / [Third International Symposium on Transport and Air Pollution]. Ed. by Robert Joumard. Organizer: French National Institute for Transport and Safety Research
Y1  - 1995
N1  - International Symposium on Transport and Air Pollution  <3, 1994, Avignon>
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Elbers, Gereon
A1  - Pfeffer, H.-U.
A1  - Friesel, J.
A1  - Beier, R.
T1  - Pilotuntersuchungen gemäß dem Verordnungsentwurf nach § 40, Abs. 2 des Bundesimmissionsschutzgesetzes / H.-U. Pfeffer, J. Friesel, G. Elbers, R. Beier, K. Ellermann
JF  - Drei Jahrzehnte Luftqualitätsüberwachung : Vorträge und Berichte zum Kolloquium "Drei Jahrzehnte Luftqualitätsüberwachung - Bilanz und Perspektiven" am 11. November 1993 in Essen / [Hrsg.: Landesanstalt für Immissionsschutz Nordrhein-Westfalen. Red. Bearb.: Robert Niesenhaus]
Y1  - 1993
N1  - LIS-Berichte ; 110 ;
SP  - 117
CY  - Essen
ER  - 
TY  - JOUR
A1  - Elbers, Gereon
A1  - Nieren, Monika
A1  - Hoffmann, Katharina
A1  - Gäb, Martina
T1  - Schnellerfassung der Wirkung von Luftschadstoffen auf Pflanzen mit der Nahinfrarotspektroskopie (NIRS). Untersuchung Ammoniak exponierter Gräser und Kräuter  / Nieren, Monika ; Hoffmann, Katharina ; Gäb, Martina ; Ooyen, Sven van ; Köllner, Barbara ; Elbe
JF  - Immissionsschutz. 13 (2008), H. 2
Y1  - 2008
SN  - 1430-9262
SP  - 82
EP  - 90
ER  - 
TY  - JOUR
A1  - Elbers, Gereon
A1  - Muratyan, Simone
T1  - Problematik verkehrsbezogener Außenluftmessungen von Partikeln (Dieselruß)
JF  - + Krebserzeugende Stoffe in der Umwelt : Herkunft, Messung, Risiko, Minimierung ; Kolloquium Mannheim, 23. bis 25. April 1991 / Kommission Reinhaltung der Luft im VDI und DIN
Y1  - 1991
SN  - 3-18-090888-2
N1  - VDI-Berichte ; 888
SP  - 143
PB  - VDI-Verl.
CY  - Düsseldorf
ER  - 
TY  - JOUR
A1  - Elbers, Gereon
A1  - Lehmann, G.
T1  - Electron paramagnetic resonance of Vanadyl ion impurities in crystalline solids: A comment
JF  - Journal of Physics and Chemistry of Solids. 46 (1985), H. 6
Y1  - 1985
SN  - 0022-3697
SP  - 761
ER  - 
TY  - JOUR
A1  - Elbers, Gereon
A1  - Lehmann, G.
T1  - Electron Paramagnetic Resonance and Optical Absorption Spectra of VO2+ in CsCl Single Crystals
JF  - Zeitschrift für Naturforschung / Section A, a journal of physical sciences. 40 (1985)
Y1  - 1985
SN  - 0932-0784
SP  - 511
ER  - 
TY  - JOUR
A1  - Elbers, Gereon
A1  - Gäb, Martina
A1  - Hoffmann, Katharina
A1  - Lobe, Melanie
T1  - NIR-spectroscopic investigation of foliage of ozone-stressed Fagus sylvatica trees / Gäb, Martina ; Hoffmann, Katharina ; Lobe, Melanie ; Metzger, Rut ; Ooyen, Sven van ; Elbers, Gereon ; Köllner, Barbara
JF  - Journal of Forest Research. 11 (2006), H. 2
Y1  - 2006
SN  - 1341-6979
SP  - 69
EP  - 75
ER  - 
TY  - JOUR
A1  - Elbers, Gereon
A1  - Buck, Manfred
T1  - Soot Concentration in Ambient Air / M. Buck, G. Elbers
JF  - Erdöl & Kohle, Erdgas, Petrochemie : EKEP. 45 (1992)
Y1  - 1992
SN  - 0014-0058
SP  - 219
ER  - 
TY  - JOUR
A1  - Elbers, Gereon
A1  - Behner, T.
A1  - Remme, S.
A1  - Prissok, F.
T1  - Interstitial Metal Impurities as a Possible Cause of Enhanced Reactivities / T. Behner, G. Elbers, S. Remme, F. Prissok, P. Stegger, and G. Lehmann
JF  - Berichte der Bunsen-Gesellschaft : physical chemistry, chemical physics. 90 (1986)
Y1  - 1986
SN  - 0005-9021
SP  - 698
ER  -