TY - CHAP A1 - Oberländer, Jan A1 - Kirchner, Patrick A1 - Keusgen, M. A1 - Schöning, Michael Josef T1 - Flexible polyimide-based calorimetric gas sensors for monitoring hy-drogen peroxide in sterilisation processes of aseptic filling machines T2 - Sensoren und Messsysteme 2014 ; Beiträge der 17. GMA/ITG-Fachtagung vom 3. bis 4. Juni 2014 in Nürnberg. (ITG-Fachbericht ; 250) Y1 - 2014 SN - 978-3-8007-3622-5 SP - 1 EP - 4 PB - VDE-Verl. CY - Düsseldorf ER - TY - JOUR A1 - Almajhdi, Fahad N. A1 - Senger, Tilo A1 - Amer, Haitham M. A1 - Gissmann, Lutz A1 - Öhlschläger, Peter T1 - Design of a highly effective therapeutic HPV16 E6/E7-specific DNA vaccine: optimization by different ways of sequence rearrangements (Shuffling) JF - PLOS one N2 - Persistent infection with the high-risk Human Papillomavirus type 16 (HPV 16) is the causative event for the development of cervical cancer and other malignant tumors of the anogenital tract and of the head and neck. Despite many attempts to develop therapeutic vaccines no candidate has entered late clinical trials. An interesting approach is a DNA based vaccine encompassing the nucleotide sequence of the E6 and E7 viral oncoproteins. Because both proteins are consistently expressed in HPV infected cells they represent excellent targets for immune therapy. Here we report the development of 8 DNA vaccine candidates consisting of differently rearranged HPV-16 E6 and E7 sequences within one molecule providing all naturally occurring epitopes but supposedly lacking transforming activity. The HPV sequences were fused to the J-domain and the SV40 enhancer in order to increase immune responses. We demonstrate that one out of the 8 vaccine candidates induces very strong cellular E6- and E7- specific cellular immune responses in mice and, as shown in regression experiments, efficiently controls growth of HPV 16 positive syngeneic tumors. This data demonstrates the potential of this vaccine candidate to control persistent HPV 16 infection that may lead to malignant disease. It also suggests that different sequence rearrangements influence the immunogenecity by an as yet unknown mechanism. Y1 - 2014 U6 - https://doi.org/10.1371/journal.pone.0113461 SN - 1932-6203 VL - 11 IS - 9 PB - PLOS CY - San Francisco ER - TY - JOUR A1 - Küppers, Tobias A1 - Steffen, Victoria A1 - Hellmuth, Hendrik A1 - O'Connell, Timothy A1 - Bongaerts, Johannes A1 - Maurer, Karl-Heinz A1 - Wiechert, Wolfgang T1 - Developing a new production host from a blueprint: Bacillus pumilus as an industrial enzyme producer JF - Microbial cell factories Y1 - 2014 U6 - https://doi.org/10.1186/1475-2859-13-46 SN - 1475-2859 (E-Journal) VL - 13 SP - Article No. 46 PB - BioMed Central CY - London ER - TY - JOUR A1 - Schroeter, Rebecca A1 - Hoffmann, Tamara A1 - Voigt, Birgit A1 - Meyer, Hanna A1 - Bleisteiner, Monika A1 - Muntel, Jan A1 - Jürgen, Britta A1 - Albrecht, Dirk A1 - Becher, Dörte A1 - Lalk, Michael A1 - Evers, Stefan A1 - Bongaerts, Johannes A1 - Maurer, Karl-Heinz A1 - Putzer, Harald A1 - Hecker, Michael A1 - Schweder, Thomas A1 - Bremer, Erhard T1 - Stress responses of the industrial workhorse Bacillus licheniformis to osmotic challenges JF - PLoS ONE N2 - The Gram-positive endospore-forming bacterium Bacillus licheniformis can be found widely in nature and it is exploited in industrial processes for the manufacturing of antibiotics, specialty chemicals, and enzymes. Both in its varied natural habitats and in industrial settings, B. licheniformis cells will be exposed to increases in the external osmolarity, conditions that trigger water efflux, impair turgor, cause the cessation of growth, and negatively affect the productivity of cell factories in biotechnological processes. We have taken here both systems-wide and targeted physiological approaches to unravel the core of the osmostress responses of B. licheniformis. Cells were suddenly subjected to an osmotic upshift of considerable magnitude (with 1 M NaCl), and their transcriptional profile was then recorded in a time-resolved fashion on a genome-wide scale. A bioinformatics cluster analysis was used to group the osmotically up-regulated genes into categories that are functionally associated with the synthesis and import of osmostress-relieving compounds (compatible solutes), the SigB-controlled general stress response, and genes whose functional annotation suggests that salt stress triggers secondary oxidative stress responses in B. licheniformis. The data set focusing on the transcriptional profile of B. licheniformis was enriched by proteomics aimed at identifying those proteins that were accumulated by the cells through increased biosynthesis in response to osmotic stress. Furthermore, these global approaches were augmented by a set of experiments that addressed the synthesis of the compatible solutes proline and glycine betaine and assessed the growth-enhancing effects of various osmoprotectants. Combined, our data provide a blueprint of the cellular adjustment processes of B. licheniformis to both sudden and sustained osmotic stress. Y1 - 2014 U6 - https://doi.org/10.1371/journal.pone.0080956 SN - 1932-6203 VL - 8 IS - 11 PB - PLOS CY - San Francisco ER - TY - JOUR A1 - Handtke, Stefan A1 - Volland, Sonja A1 - Methling, Karen A1 - Albrecht, Dirk A1 - Becher, Dörte A1 - Nehls, Jenny A1 - Bongaerts, Johannes A1 - Maurer, Karl-Heinz A1 - Lalk, Michael A1 - Liesegang, Heiko A1 - Voigt, Birgit A1 - Daniel, Rolf A1 - Hecker, Michael T1 - Cell physiology of the biotechnological relevant bacterium Bacillus pumilus - An omics-based approach JF - Journal of Biotechnology N2 - Members of the species Bacillus pumilus get more and more in focus of the biotechnological industry as potential new production strains. Based on exoproteome analysis, B. pumilus strain Jo2, possessing a high secretion capability, was chosen for an omics-based investigation. The proteome and metabolome of B. pumilus cells growing either in minimal or complex medium was analyzed. In total, 1542 proteins were identified in growing B. pumilus cells, among them 1182 cytosolic proteins, 297 membrane and lipoproteins and 63 secreted proteins. This accounts for about 43% of the 3616 proteins encoded in the B. pumilus Jo2 genome sequence. By using GC–MS, IP-LC/MS and H NMR methods numerous metabolites were analyzed and assigned to reconstructed metabolic pathways. In the genome sequence a functional secretion system including the components of the Sec- and Tat-secretion machinery was found. Analysis of the exoproteome revealed secretion of about 70 proteins with predicted secretion signals. In addition, selected production-relevant genome features such as restriction modification systems and NRPS clusters of B. pumilus Jo2 are discussed. Y1 - 2014 U6 - https://doi.org/10.1016/j.jbiotec.2014.08.028 SN - 1873-4863 (E-Journal); 0168-1656 (Print) IS - 192(A) SP - 204 EP - 214 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Poghossian, Arshak A1 - Schöning, Michael Josef T1 - Label-free sensing of biomolecules with field-effect devices for clinical applications JF - Electroanalysis N2 - Among the variety of transducer concepts proposed for label-free detection of biomolecules, the semiconductor field-effect device (FED) is one of the most attractive platforms. As medical techniques continue to progress towards diagnostic and therapies based on biomarkers, the ability of FEDs for a label-free, fast and real-time detection of multiple pathogenic and physiologically relevant molecules with high specificity and sensitivity offers very promising prospects for their application in point-of-care and personalized medicine for an early diagnosis and treatment of diseases. The presented paper reviews recent advances and current trends in research and development of different FEDs for label-free, direct electrical detection of charged biomolecules by their intrinsic molecular charge. The authors are mainly focusing on the detection of the DNA hybridization event, antibody-antigen affinity reaction as well as clinically relevant biomolecules such as cardiac and cancer biomarkers. Y1 - 2014 U6 - https://doi.org/10.1002/elan.201400073 SN - 1521-4109 (E-Journal); 1040-0397 (Print) VL - 26 IS - 6 SP - 1197 EP - 1213 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Leinhos, Marcel A1 - Schusser, Sebastian A1 - Bäcker, Matthias A1 - Poghossian, Arshak A1 - Schöning, Michael Josef T1 - Micromachined multi-parameter sensor chip for the control of polymer-degradation medium JF - Physica Status Solidi (A) : special issue on engineering and functional interfaces N2 - It is well known that the degradation environment can strongly influence the biodegradability and kinetics of biodegradation processes of polymers. Therefore, besides the monitoring of the degradation process, it is also necessary to control the medium in which the degradation takes place. In this work, a micromachined multi-parameter sensor chip for the control of the polymer-degradation medium has been developed. The chip combines a capacitive field-effect pH sensor, a four-electrode electrolyte-conductivity sensor and a thin-film Pt-temperature sensor. The results of characterization of individual sensors are presented. In addition, the multi-parameter sensor chip together with an impedimetric polymer-degradation sensor was simultaneously characterized in degradation solutions with different pH and electrolyte conductivity. The obtained results demonstrate the feasibility of the multi-parameter sensor chip for the control of the polymer-degradation medium. Y1 - 2014 U6 - https://doi.org/10.1002/pssa.201330364 SN - 1521-396X (E-Journal); 1862-6319 (E-Journal); 0031-8965 (Print); 1862-6300 (Print) VL - 211 IS - 6 SP - 1346 EP - 1351 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Huck, Christina A1 - Poghossian, Arshak A1 - Bäcker, Matthias A1 - Chaudhuri, S. A1 - Zander, W. A1 - Schubert, J. A1 - Begoyan, V. K. A1 - Buniatyan, V. V. A1 - Wagner, P. A1 - Schöning, Michael Josef T1 - Capacitively coupled electrolyte-conductivity sensor based on high-k material of barium strontium titanate JF - Sensors and actuators. B: Chemical Y1 - 2014 U6 - https://doi.org/10.1016/j.snb.2014.02.103 SN - 1873-3077 (E-Journal); 0925-4005 (Print) IS - 198 SP - 102 EP - 109 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Bäcker, Matthias A1 - Kramer, F. A1 - Huck, Christina A1 - Poghossian, Arshak A1 - Bratov, A. A1 - Abramova, N. A1 - Schöning, Michael Josef T1 - Planar and 3D interdigitated electrodes for biosensing applications: The impact of a dielectric barrier on the sensor properties JF - Physica Status Solidi (A) - Applications and Materials Science N2 - Planar and three-dimensional (3D) interdigitated electrodes (IDE) with electrode digits separated by an insulating barrier of different heights were electrochemically characterized and compared in terms of their sensing properties. Due to the impact of the surface resistance, both types of IDE structures display a non-linear behavior in low-ionic strength solutions. The experimental data were fitted to an electrical equivalent circuit and interpreted taking into account the surface-charge-governed properties. The effect of a charged polyelectrolyte layer electrostatically assembled onto the sensor surface on the surface resistance in solutions with different KCl concentration is studied. In case of the same electrode footprint, 3D-IDEs show a larger cell constant and a higher sensitivity to molecular adsorption than that of planar IDEs. The obtained results demonstrate the potential of 3D-IDEs as a new transducer structure for a direct label-free sensing of charged molecules. Y1 - 2014 U6 - https://doi.org/10.1002/pssa.201330416 SN - 1521-396X (E-Journal); 1862-6319 (E-Journal); 0031-8965 (Print); 1862-6300 (Print) VL - 211 IS - 6 SP - 1357 EP - 1363 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Moseley, Fiona A1 - Halamek, Jan A1 - Kramer, Friederike A1 - Poghossian, Arshak A1 - Schöning, Michael Josef A1 - Katz, Evgeny T1 - An enzyme-based reversible CNOT logic gate realized in a flow system JF - Analyst N2 - An enzyme system organized in a flow device was used to mimic a reversible Controlled NOT (CNOT) gate with two input and two output signals. Reversible conversion of NAD⁺ and NADH cofactors was used to perform a XOR logic operation, while biocatalytic hydrolysis of p-nitrophenyl phosphate resulted in an Identity operation working in parallel. The first biomolecular realization of a CNOT gate is promising for integration into complex biomolecular networks and future biosensor/biomedical applications. Y1 - 2014 U6 - https://doi.org/10.1039/C4AN00133H SN - 1364-5528 (E-Journal) ; 0003-2654 (Print) VL - 139 IS - 8 SP - 1839 EP - 1842 PB - Royal Society of Chemistry CY - Cambridge ER - TY - JOUR A1 - Huck, Christina A1 - Poghossian, Arshak A1 - Kerroumi, Iman A1 - Schusser, Sebastian A1 - Bäcker, Matthias A1 - Zander, Willi A1 - Schubert, Jürgen A1 - Buniatyan, Vahe V. A1 - Martirosyan, Norayr W. A1 - Wagner, Patrick A1 - Schöning, Michael Josef T1 - Multiparameter sensor chip with Barium Strontium Titanate as multipurpose material JF - Electroanalysis N2 - It is well known that biochemical and biotechnological processes are strongly dependent and affected by a variety of physico-chemical parameters such as pH value, temperature, pressure and electrolyte conductivity. Therefore, these quantities have to be monitored or controlled in order to guarantee a stable process operation, optimization and high yield. In this work, a sensor chip for the multiparameter detection of three physico-chemical parameters such as electrolyte conductivity, pH and temperature is realized using barium strontium titanate (BST) as multipurpose material. The chip integrates a capacitively coupled four-electrode electrolyte-conductivity sensor, a capacitive field-effect pH sensor and a thin-film Pt-temperature sensor. Due to the multifunctional properties of BST, it is utilized as final outermost coating layer of the processed sensor chip and serves as passivation and protection layer as well as pH-sensitive transducer material at the same time. The results of testing of the individual sensors of the developed multiparameter sensor chip are presented. In addition, a quasi-simultaneous multiparameter characterization of the sensor chip in buffer solutions with different pH value and electrolyte conductivity is performed. To study the sensor behavior and the suitability of BST as multifunctional material under harsh environmental conditions, the sensor chip was exemplarily tested in a biogas digestate. Y1 - 2014 U6 - https://doi.org/10.1002/elan.201400076 SN - 1521-4109 (E-Journal); 1040-0397 (Print) VL - 26 IS - 5 SP - 980 EP - 987 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Wu, Chunsheng A1 - Bronder, Thomas A1 - Poghossian, Arshak A1 - Werner, Frederik A1 - Bäcker, Matthias A1 - Schöning, Michael Josef T1 - Label-free electrical detection of DNA with a multi-spot LAPS: First step towards light-addressable DNA chips JF - Physica status solidi A : Applications and materials science N2 - A multi-spot (4 × 4 spots) light-addressable potentiometric sensor (MLAPS) consisting of an Al–p-Si–SiO2 structure has been applied for the label-free electrical detection of DNA (deoxyribonucleic acid) immobilization and hybridization by the intrinsic molecular charge for the first time. Single-stranded probe ssDNA molecules (20 bases) were covalently immobilized onto the silanized SiO2 gate surface. The unspecific adsorption of mismatch ssDNA on the MLAPS gate surface was blocked by bovine serum albumin molecules. To reduce the screening effect and to achieve a high sensor signal, the measurements were performed in a low ionic-strength solution. The photocurrent–voltage (I–V) curves were simultaneously recorded on all 16 spots after each surface functionalization step. Large shifts of I–V curves of 25 mV were registered after the DNA immobilization and hybridization event. In contrast, a small potential shift (∼5 mV) was observed in case of mismatch ssDNA, revealing good specificity of the sensor. The obtained results demonstrate the potential of the MLAPS as promising transducer platform for the multi-spot label-free electrical detection of DNA molecules by their intrinsic molecular charge. Y1 - 2014 U6 - https://doi.org/10.1002/pssa.201330442 SN - 1521-396X (E-Journal); 1862-6319 (E-Journal); 0031-8965 (Print); 1862-6300 (Print) VL - 211 IS - 6 SP - 1423 EP - 1428 PB - Wiley-VCH CY - Weinheim ER - TY - CHAP A1 - Wu, Chunsheng A1 - Bronder, Thomas A1 - Poghossian, Arshak A1 - Schöning, Michael Josef T1 - DNA-hybridization detection using light-addressable potentiometric sensor modified with gold layer T2 - Sensoren und Messsysteme 2014 ; Beiträge der 17. GMA/ITG-Fachtagung vom 3. bis 4. Juni 2014 in Nürnberg. (ITG-Fachbericht ; 250) Y1 - 2014 SN - 978-3-8007-3622-5 SP - 1 EP - 4 PB - VDE-Verl. CY - Düsseldorf ER - TY - CHAP A1 - Huck, Christina A1 - Poghossian, Arshak A1 - Buniatyan, V. A1 - Schöning, Michael Josef T1 - Multi-parameter detection for supporting monitoring and control of biogas processes in agriculture T2 - Sensoren und Messsysteme 2014 ; Beiträge der 17. GMA/ITG-Fachtagung vom 3. bis 4. Juni 2014 in Nürnberg. (ITG-Fachbericht ; 250) Y1 - 2014 SN - 978-3-8007-3622-5 SP - 1 EP - 5 PB - VDE-Verl. CY - Berlin ER - TY - CHAP A1 - Schöning, Michael Josef A1 - Poghossian, Arshak A1 - Glück, Olaf A1 - Thust, Marion T1 - Electrochemical methods for the determination of chemical variables in aqueous media T2 - Measurement, instrumentation, and sensors handbook / ed. by John G. Webster [u.a.] Vol. 2 : Electromagnetic, optical, radiation, chemical, and biomedical measurement Y1 - 2014 SN - 978-1-4398-4891-3 SP - 55-1 EP - 55-54 PB - CRC Pr. CY - Boca Raton, Fla. ER - TY - CHAP A1 - Buniatyan, V. V. A1 - Huck, Christina A1 - Poghossian, Arshak A1 - Schöning, Michael Josef A1 - Rustamyan, L. G. A1 - Hovnikyan, H. H. T1 - Equivalent circuit and optimization of impedance characteristics of an electrolyte conductivity sensor T2 - Proceedings of State Engineering University Armenia : Series Information technologies, electronics, radio engineering Y1 - 2014 VL - Iss. 17 IS - No. 1 SP - 69 EP - 76 ER - TY - CHAP A1 - Bäcker, Matthias A1 - Schusser, Sebastian A1 - Leinhos, Marcel A1 - Poghossian, Arshak A1 - Schöning, Michael Josef T1 - Sensor system for the monitoring of degradation processes of biodegradable biopolymers T2 - Sensoren und Messsysteme 2014 ; Beiträge der 17. GMA/ITG-Fachtagung vom 3. bis 4. Juni 2014 in Nürnberg. (ITG-Fachbericht ; 250) Y1 - 2014 SN - 978-3-8007-3622-5 SP - 1 EP - 4 PB - VDE-Verl. CY - Düsseldorf ER - TY - JOUR A1 - Bäcker, Matthias A1 - Schusser, Sebastian A1 - Poghossian, Arshak A1 - Schöning, Michael Josef T1 - Multi-Parametererfassung mit siliziumbasiertem Sensorchip: Aus Drei mach Eins JF - GIT Labor-Fachzeitschrift Y1 - 2014 SN - 0016-3538 IS - 2 SP - 28 EP - 30 PB - Wiley ER - TY - CHAP A1 - Poghossian, Arshak A1 - Weiland, Maryam A1 - Schöning, Michael Josef ED - Lvova, Larisa ED - Kirsanov, Dmitry ED - di Natale, Corrado ED - Legin, Audrey T1 - Nanoplate field-effect capacitors: a new transducer structure for multiparameter (bio-)chemical sensing T2 - Multisensor system for chemical analysis : materials and sensors N2 - An array of electrically isolated nanoplate field-effect silicon-on-insulator (SOI) capacitors as a new transducer structure for multiparameter (bio-)chemical sensing is presented. The proposed approach allows addressable biasing and electrical readout of multiple nanoplate field-effect capacitive (bio-)chemical sensors on the same SOI chip, as well as differential-mode measurements. The realized sensor chip has been applied for pH and penicillin concentration measurements, electrical monitoring of polyelectrolyte multilayer formation, and the label-free electrical detection of consecutive deoxyribonucleic acid (DNA) hybridization and denaturation events. Y1 - 2014 SN - 978-981-4411-15-8 ; 978-981-4411-16-5 U6 - https://doi.org/10.1201/b15491-11 SP - 333 EP - 373 PB - Jenny Stanford Publishing CY - Singapore ET - 1 ER - TY - JOUR A1 - Bohrn, Ulrich A1 - Stütz, Evamaria A1 - Fleischer, Maximilian A1 - Schöning, Michael Josef A1 - Wagner, Patrick T1 - Using a cell-based gas biosensor for investigation of adverse effects of acetone vapors in vitro JF - Biosensors and Bioelectronics. 40 (2013), H. 1 Y1 - 2013 SN - 0956-5663 SP - 393 EP - 400 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Hennemann, Jörg A1 - Kohl, Claus-Dieter A1 - Reisert, Steffen A1 - Kirchner, Patrick A1 - Schöning, Michael Josef T1 - Copper oxide nanofibres for detection of hydrogen peroxide vapour at high concentrations JF - physica status solidi (a) N2 - We present a sensor concept based on copper(II)oxide (CuO) nanofibres for the detection of hydrogen peroxide (H2O2) vapour in the percent per volume (% v/v) range. The fibres were produced by using the electrospinning technique. To avoid water condensation in the pores, the fibres were initially modified by an exposure to H2S to get an enclosed surface. By a thermal treatment at 350 °C the fibres were oxidised back to CuO. Thereby, the visible pores disappear which was verified by SEM analysis. The fibres show a decrease of resistance with increasing H2O2 concentration which is due to the fact that hydrogen peroxide is an oxidising gas and CuO a p-type semiconductor. The sensor shows a change of resistance within the minute range to the exposure until the maximum concentration of 6.9% v/v H2O2. At operating temperatures below 450 °C the corresponding sensor response to a concentration of 4.1% v/v increases. The sensor shows a good reproducibility of the signal at different measurements. CuO seems to be a suitable candidate for the detection of H2O2 vapour at high concentrations. Resistance behaviour of the sensor under exposure to H2O2 vapours between 2.3 and 6.9% v/v at an operating temperature of 450 °C. Y1 - 2013 U6 - https://doi.org/10.1002/pssa.201200775 SN - 1862-6319 VL - 210 IS - 5 SP - 859 EP - 863 PB - Wiley CY - Weinheim ER - TY - JOUR A1 - Reisert, Steffen A1 - Schneider, Benno A1 - Geissler, Hanno A1 - Gompel, Matthias van A1 - Wagner, Patrick A1 - Schöning, Michael Josef T1 - Multi-sensor chip for the investigation of different types of metal oxides for the detection of H2O2 in the ppm range JF - physica status solidi (a) N2 - In this work, a multi-sensor chip for the investigation of the sensing properties of different types of metal oxides towards hydrogen peroxide in the ppm range is presented. The fabrication process and physical characterization of the multi-sensor chip are described. Pure SnO2 and WO3 as well as Pd- and Pt-doped SnO2 films are characterized in terms of their sensitivity to H2O2. The sensing films have been prepared by drop-coating of water-dispensed nano-powders. A physical characterization, including scanning electron microscopy and X-ray diffraction analysis of the deposited metal-oxide films, was done. From the measurements in hydrogen peroxide atmosphere, it could be shown, that all of the tested metal oxide films are suitable for the detection of H2O2 in the ppm range. The highest sensitivity and reproducibility was achieved using Pt-doped SnO2. Calibration plot of a SnO2, WO3, Pt-, and Pd-doped SnO2 gas sensor for H2O2 concentrations in the ppm range. Y1 - 2013 SN - 1862-6319 VL - 210 IS - 5 SP - 898 EP - 904 PB - Wiley CY - Weinheim ER - TY - JOUR A1 - Kirchner, Patrick A1 - Oberländer, Jan A1 - Suso, Henri-Pierre A1 - Rysstad, Gunnar A1 - Keusgen, Michael A1 - Schöning, Michael Josef T1 - Towards a wireless sensor system for real-time H2O2 monitoring in aseptic food processes JF - Physica status solidi (a) N2 - A wireless sensor system based on the industrial ZigBee standard for low-rate wireless networking was developed that enables real-time monitoring of gaseous H2O2 during the package sterilization in aseptic food processes. The sensor system consists of a remote unit connected to a calorimetric gas sensor, which was already established in former works, and an external base unit connected to a laptop computer. The remote unit was built up by an XBee radio frequency (RF) module for data communication and a programmable system-on-chip controller to read out the sensor signal and process the sensor data, whereas the base unit is a second XBee RF module. For the rapid H2O2 detection on various locations inside the package that has to be sterilized, a novel read-out strategy of the calorimetric gas sensor was established, wherein the sensor response is measured within the short sterilization time and correlated with the present H2O2 concentration. In an exemplary measurement application in an aseptic filling machinery, the suitability of the new, wireless sensor system was demonstrated, wherein the influence of the gas velocity on the H2O2 distribution inside a package was determined and verified with microbiological tests. KW - calorimetric gas sensor;hydrogen peroxide;wireless sensor system Y1 - 2013 U6 - https://doi.org/10.1002/pssa.201200920 SN - 1862-6319 VL - 210 IS - 5 SP - 877 EP - 883 PB - Wiley CY - Weinheim ER - TY - JOUR A1 - Miyamoto, Ko-ichiro A1 - Ichimura, Hiroki A1 - Wagner, Torsten A1 - Schöning, Michael Josef A1 - Yoshinobu, Tatsuo T1 - Chemical imaging of the concentration profile of ion diffusion in a microfluidic channel JF - Sensors and actuators. B: Chemical N2 - The chemical imaging sensor is a device to visualize the spatial distribution of chemical species based on the principle of LAPS (light-addressable potentiometric sensor), which is a field-effect chemical sensor based on semiconductor. In this study, the chemical imaging sensor has been applied to investigate the ion profile of laminar flows in a microfluidic channel. The chemical images (pH maps) were collected in a Y-shaped microfluidic channel while injecting HCl and NaCl solutions into two branches. From the chemical images, it was clearly observed that the injected solutions formed laminar flows in the channel. In addition, ion diffusion across the laminar flows was observed, and the diffusion coefficient could be derived by fitting the pH profiles to the Fick's equation. Y1 - 2013 U6 - https://doi.org/10.1016/j.snb.2013.04.057 SN - 1873-3077 (E-Journal); 0925-4005 (Print) N1 - Part of special issue "Selected Papers from the 26th European Conference on Solid-State Transducers" VL - 189 SP - 240 EP - 245 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Bohrn, Ulrich A1 - Mucha, Andreas A1 - Werner, Frederik A1 - Trattner, Barbara A1 - Bäcker, Matthias A1 - Krumbe, Christoph A1 - Schienle, Meinrad A1 - Stütz, Evamaria A1 - Schmitt-Landsiedel, Doris A1 - Fleischer, Maximilian A1 - Wagner, Patrick A1 - Schöning, Michael Josef T1 - A critical comparison of cell-based sensor systems for the detection of Cr (VI) in aquatic environment JF - Sensors and actuators. B: Chemical Y1 - 2013 SN - 1873-3077 (E-Journal); 0925-4005 (Print) VL - Vol. 182 SP - 58 EP - 65 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Werner, Frederik A1 - Takenaga, Shoko A1 - Taki, Hidenori A1 - Sawada, Kazuaki A1 - Schöning, Michael Josef T1 - Comparison of label-free ACh-imaging sensors based on CCD and LAPS JF - Sensors and Actuators B: Chemical (2012) N2 - Semiconductor-based chemical imaging sensors, like the light-addressable potentiometric sensor (LAPS) or the pH-imaging sensor based on a charge-coupled device (CCD), are becoming a powerful tool for label-free imaging of biological phenomena. We have proposed a polyion-based enzymatic membrane to develop an acetylcholine (ACh) imaging sensor for neural cell-activity observations. In this study, a CCD-type ACh-imaging sensor and a LAPS-type ACh-imaging sensor were fabricated and the prospect of both sensors was clarified by making a comparison of their basic characteristics. Y1 - 2013 SN - 0925-4005 VL - 177 SP - 745 EP - 752 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Kirchner, Patrick A1 - Oberländer, Jan A1 - Suco, Henri-Pierre A1 - Rysstad, Gunnar A1 - Schöning, Michael Josef T1 - Monitoring the microbicidal effectiveness of gaseous hydrogen peroxide in sterilisation processes by means of a calorimetric gas sensor JF - Food control N2 - In the present work, a novel method for monitoring sterilisation processes with gaseous H2O2 in combination with heat activation by means of a specially designed calorimetric gas sensor was evaluated. Therefore, the sterilisation process was extensively studied by using test specimens inoculated with Bacillus atrophaeus spores in order to identify the most influencing process factors on its microbicidal effectiveness. Besides the contact time of the test specimens with gaseous H2O2 varied between 0.2 and 0.5 s, the present H2O2 concentration in a range from 0 to 8% v/v (volume percent) had a strong influence on the microbicidal effectiveness, whereas the change of the vaporiser temperature, gas flow and humidity were almost negligible. Furthermore, a calorimetric H2O2 gas sensor was characterised in the sterilisation process with gaseous H2O2 in a wide range of parameter settings, wherein the measurement signal has shown a linear response against the H2O2 concentration with a sensitivity of 4.75 °C/(% v/v). In a final step, a correlation model by matching the measurement signal of the gas sensor with the microbial inactivation kinetics was established that demonstrates its suitability as an efficient method for validating the microbicidal effectiveness of sterilisation processes with gaseous H2O2. KW - hydrogen peroxide KW - sterilisation KW - Bacillus atrophaeus KW - calorimetric gas sensor Y1 - 2012 U6 - https://doi.org/10.1016/j.foodcont.2012.11.048 SN - 0956-7135 VL - 31 IS - 2 SP - 530 EP - 538 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Werner, Frederik A1 - Wagner, Torsten A1 - Yoshinobu, Tatsuo A1 - Keusgen, Michael A1 - Schöning, Michael Josef T1 - Frequency behaviour of light-addressable potentiometric sensors JF - Physica Status Solidi (A) N2 - Light-addressable potentiometric sensors (LAPS) are semiconductor-based potentiometric sensors, with the advantage to detect the concentration of a chemical species in a liquid solution above the sensor surface in a spatially resolved manner. The addressing is achieved by a modulated and focused light source illuminating the semiconductor and generating a concentration-depending photocurrent. This work introduces a LAPS set-up that is able to monitor the electrical impedance in addition to the photocurrent. The impedance spectra of a LAPS structure, with and without illumination, as well as the frequency behaviour of the LAPS measurement are investigated. The measurements are supported by electrical equivalent circuits to explain the impedance and the LAPS-frequency behaviour. The work investigates the influence of different parameters on the frequency behaviour of the LAPS. Furthermore, the phase shift of the photocurrent, the influence of the surface potential as well as the changes of the sensor impedance will be discussed. Y1 - 2013 U6 - https://doi.org/10.1002/pssa.201200929 SN - 1521-396X ; 0031-8965 VL - 210 IS - 5 SP - 884 EP - 891 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Murib, M. S. A1 - Grinsven, B. van A1 - Grieten, L. A1 - Janssens, S. D. A1 - Vermeeren, V. A1 - Eersels, K. A1 - Broeders, J. A1 - Ameloot, M. A1 - Michiels, L. A1 - Ceuninck, W. De A1 - Haenen, K. A1 - Schöning, Michael Josef A1 - Wagner, Patrick T1 - Electronic monitoring of chemical DNA denaturation on nanocrystalline diamond electrodes with different molarities and flow rates JF - Physica Status Solidi (A). Vol. 210 (2013), iss. 5 Y1 - 2013 SN - 0031-8965 SP - 911 EP - 917 PB - Wiley-VCH CY - Berlin ER - TY - JOUR A1 - Schiffels, Johannes A1 - Pinkenburg, Olaf A1 - Schelden, Maximilian A1 - Aboulnaga, El-Hussiny A. A. A1 - Baumann, Marcus A1 - Selmer, Thorsten T1 - An innovative cloning platform enables large-scale production and maturation of an oxygen-tolerant [NiFe]-hydrogenase from cupriavidus necator in Escherichia coli JF - PLOS one. 2013 Y1 - 2013 U6 - https://doi.org/10.1371/journal.pone.0068812 SN - 1932-6203 PB - Public Library of Science CY - San Francisco, California ER - TY - JOUR A1 - Itabashi, Akinori A1 - Kosaka, Naoki A1 - Miyamoto, Ko-ichiro A1 - Wagner, Torsten A1 - Schöning, Michael Josef T1 - High-speed chemical imaging system based on front-side-illuminated LAPS JF - Sensors and actuators B: Chemical N2 - The chemical imaging sensor is a semiconductor-based chemical sensor that can visualize the spatial distribution of specific ions on the sensing surface. The conventional chemical imaging system based on the light-addressable potentiometric sensor (LAPS), however, required a long time to obtain a chemical image, due to the slow mechanical scan of a single light beam. For high-speed imaging, a plurality of light beams modulated at different frequencies can be employed to measure the ion concentrations simultaneously at different locations on the sensor plate by frequency division multiplex (FDM). However, the conventional measurement geometry of back-side illumination limited the bandwidth of the modulation frequency required for FDM measurement, because of the low-pass filtering characteristics of carrier diffusion in the Si substrate. In this study, a high-speed chemical imaging system based on front-side-illuminated LAPS was developed, which achieved high-speed spatiotemporal recording of pH change at a rate of 70 frames per second. Y1 - 2013 U6 - https://doi.org/10.1016/j.snb.2013.03.016 SN - 1873-3077 VL - 182 SP - 315 EP - 321 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Iken, Heiko A1 - Ahlborn, K. A1 - Gerlach, F. A1 - Vonau, W. A1 - Zander, W. A1 - Schubert, J. A1 - Schöning, Michael Josef T1 - Development of redox glasses and subsequent processing by means of pulsed laser deposition for realizing silicon-based thin-film sensors JF - Electrochimica acta Y1 - 2013 SN - 1873-3859 (E-Journal); 0013-4686 (Print) SP - Available online 30.8.2013 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Reisert, Steffen A1 - Geissler, Hanno A1 - Flörke, Rudolf A1 - Weiler, Christian A1 - Wagner, Patrick A1 - Schöning, Michael Josef T1 - Characterisation of aseptic sterilisation processes using an electronic nose JF - International journal of nanotechnology Y1 - 2013 SN - 1475-7435 (Print) 7141-8151 (Online) VL - Vol. 10 IS - No. 5-7 SP - 470 EP - 484 PB - Inderscience Enterprises CY - Genève ER - TY - JOUR A1 - Miyamoto, Ko-ichiro A1 - Hirayama, Yuji A1 - Wagner, Torsten A1 - Schöning, Michael Josef A1 - Yoshinobu, Tatsuo T1 - Visualization of enzymatic reaction in a microfluidic channel using chemical imaging sensor JF - Electrochimica acta Y1 - 2013 SN - 1873-3859 (E-Journal); 0013-4686 (Print) SP - Publ. online PB - Elsevier CY - Amsterdam ER - TY - GEN A1 - Ingebrandt, Sven A1 - Wagner, Patrick A1 - Schöning, Michael Josef T1 - Engineering of functional interfaces / guest eds. Sven Ingebrandt ; Patrick Wagner ; Michael J. Schöning T2 - Physica Status Solidi (A) Y1 - 2013 SN - 1521-396X (E-Journal); 1862-6319 (E-Journal); 0031-8965 (Print); 1862-6300 (Print) VL - Vol. 210 IS - Iss. 5 SP - 845 ER - TY - GEN A1 - Abdelghani, Adnane A1 - Menaa, Bouzid A1 - Obare, Sherine O. A1 - Schöning, Michael Josef T1 - Special issue on nanoscale science and technology / guest eds.: Adnane Abdelghani, Bouzid Menaa, Sherine O. Obare, Michael J. Schöning T2 - International journal of nanotechnology Y1 - 2013 SN - 1741-8151 (E-Journal), 1475-7435 (Print) VL - Vol. 10 IS - No. 5-7 SP - 373 EP - 619 ER - TY - JOUR A1 - Sabitova, A. A1 - Ebert, Ph. A1 - Lenz, A. A1 - Schaafhausen, S. A1 - Ivanova, L. A1 - Dähne, M. A1 - Hoffmann, A. A1 - Dunin-Borkowski, R. E. A1 - Förster, Arnold A1 - Grandidier, B. A1 - Eisele, H. T1 - Intrinsic bandgap of cleaved ZnO(112¯0) surfaces JF - Applied physics letters Y1 - 2013 SN - 1077-3118 (E-Journal); 0003-6951 (Print) VL - Vol. 102 SP - 021608 ER - TY - CHAP A1 - Takenaga, Shoko A1 - Herrera, Cony F. A1 - Werner, Frederik A1 - Biselli, Manfred A1 - Schnitzler, Thomas A1 - Schöning, Michael Josef A1 - Öhlschläger, Peter A1 - Wagner, Torsten T1 - Detection of the metabolic activity of cells by differential measurements based on a single light-addressable potentiometric sensor chip T2 - 11. Dresdner Sensor-Symposium : 9.-11.12.2013 Y1 - 2013 SN - 978-3-9813484-5-3 SP - 63 EP - 67 ER - TY - CHAP A1 - Oberländer, Jan A1 - Reisert, Steffen A1 - Kirchner, Patrick A1 - Wagner, Patrick A1 - Schöning, Michael Josef T1 - Kalorimetrische Gassensoren zur H2O2-Detektion in aseptischen Sterilisationsprozessen T2 - 11. Dresdner Sensor-Symposium : 9.-11.12.2013 Y1 - 2013 SN - 978-3-9813484-5-3 SP - 234 EP - 238 ER - TY - JOUR A1 - Martinez, Ronny A1 - Jakob, Felix A1 - Tu, Ran A1 - Siegert, Petra A1 - Maurer, Karl-Heinz A1 - Schwaneberg, Ulrich T1 - Increasing activity and thermal resistance of Bacillus gibsonii alkaline protease (BgAP) by directed evolution JF - Biotechnology and bioengineering Y1 - 2013 SN - 1097-0290 (E-Journal); 0006-3592 (Print); 0368-1467 (Print) VL - Vol. 110 IS - Iss. 3 SP - 711 EP - 720 PB - Wiley CY - Weinheim ER - TY - CHAP A1 - Kreyer, Jörg A1 - Stollenwerk, Dominik A1 - Schöning, Michael Josef T1 - Tagung des Forschernachwuchses der FH Aachen 20. November 2013 / ed.: J. Kreyer, D. Stollenwerk, M. J. Schöning Y1 - 2013 PB - FH Aachen CY - Aachen ER - TY - JOUR A1 - Rachinger, Michael A1 - Bauch, Melanie A1 - Strittmatter, Axel A1 - Bongaerts, Johannes A1 - Evers, Stefan A1 - Maurer, Karl-Heinz A1 - Daniel, Rolf A1 - Liebl, Wolfgang A1 - Liesegang, Heiko A1 - Ehrenreich, Armin T1 - Size unlimited markerless deletions by a transconjugative plasmid-system in Bacillus licheniformis JF - Journal of biotechnology Y1 - 2013 SN - 1873-4863 (E-Journal); 0168-1656 (Print) VL - Vol. 164 IS - Iss. 4 SP - 365 EP - 369 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Wilming, Anja A1 - Begemann, Jens A1 - Kuhne, Stefan A1 - Regestein, Lars A1 - Bongaerts, Johannes A1 - Evers, Stefan A1 - Maurer, Karl-Heinz A1 - Büchs, Jochen T1 - Metabolic studies of γ-polyglutamic acid production in Bacillus licheniformis by small-scale continuous cultivations JF - Biochemical engineering journal Y1 - 2013 SN - 1873-295X (E-Journal); 1369-703X (Print) VL - Vol. 73 SP - 29 EP - 37 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Scheele, Sandra A1 - Oertel, Dan A1 - Bongaerts, Johannes A1 - Evers, Stefan A1 - Hellmuth, Hendrik A1 - Maurer, Karl-Heinz A1 - Bott, Michael A1 - Freudl, Roland T1 - Secretory production of an FAD cofactor-containing cytosolic enzyme (sorbitol–xylitol oxidase from Streptomyces coelicolor) using the twin-arginine translocation (Tat) pathway of Corynebacterium glutamicum JF - Microbial biotechnology Y1 - 2013 SN - 1751-7915 SP - 202 EP - 206 PB - Wiley-Blackwell CY - Oxford ER - TY - JOUR A1 - Voigt, Birgit A1 - Schroeter, Rebecca A1 - Jürgen, Britta A1 - Albrecht, Dirk A1 - Evers, Stefan A1 - Bongaerts, Johannes A1 - Maurer, Karl-Heinz A1 - Schweder, Thomas A1 - Hecker, Michael T1 - The response of Bacillus licheniformis to heat and ethanol stress and the role of the SigB regulon JF - Proteomics Y1 - 2013 SN - 1615-9861 (E-Journal); 1615-9853 (Print) VL - Vol. 13 IS - Iss. 14 SP - 2140 EP - 2146 PB - Wiley CY - Weinheim ER - TY - JOUR A1 - Wiegand, Sandra A1 - Dietrich, Sascha A1 - Hertel, Robert A1 - Bongaerts, Johannes A1 - Evers, Stefan A1 - Volland, Sonja A1 - Daniel, Rolf A1 - Liesegang, Heiko T1 - RNA-Seq of Bacillus licheniformis: active regulatory RNA features expressed within a productive fermentation JF - BMC genomics Y1 - 2013 SN - 1471-2164 VL - Vol. 14 SP - 667 PB - BioMed Central CY - London ER - TY - CHAP A1 - Wu, Chunsheng A1 - Poghossian, Arshak A1 - Werner, Frederik A1 - Bronder, Thomas A1 - Bäcker, Matthias A1 - Wang, Ping A1 - Schöning, Michael Josef T1 - An application of a scanning light-addressable potentiometric sensor for label-free DNA detection T2 - 11. Dresdner Sensor-Symposium : 9.-11.12.2013 Y1 - 2013 SN - 978-3-9813484-5-3 SP - 164 EP - 168 ER - TY - JOUR A1 - Huck, Christina A1 - Schiffels, Johannes A1 - Herrera, Cony N. A1 - Schelden, Maximilian A1 - Selmer, Thorsten A1 - Poghossian, Arshak A1 - Baumann, Marcus A1 - Wagner, Patrick A1 - Schöning, Michael Josef T1 - Metabolic responses of Escherichia coli upon glucose pulses captured by a capacitive field-effect sensor JF - Physica Status Solidi (A) N2 - Living cells are complex biological systems transforming metabolites taken up from the surrounding medium. Monitoring the responses of such cells to certain substrate concentrations is a challenging task and offers possibilities to gain insight into the vitality of a community influenced by the growth environment. Cell-based sensors represent a promising platform for monitoring the metabolic activity and thus, the “welfare” of relevant organisms. In the present study, metabolic responses of the model bacterium Escherichia coli in suspension, layered onto a capacitive field-effect structure, were examined to pulses of glucose in the concentration range between 0.05 and 2 mM. It was found that acidification of the surrounding medium takes place immediately after glucose addition and follows Michaelis–Menten kinetic behavior as a function of the glucose concentration. In future, the presented setup can, therefore, be used to study substrate specificities on the enzymatic level and may as well be used to perform investigations of more complex metabolic responses. Conclusions and perspectives highlighting this system are discussed. Y1 - 2013 U6 - https://doi.org/10.1002/pssa.201200900 SN - 0031-8965 VL - 210 IS - 5 SP - 926 EP - 931 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Schusser, Sebastian A1 - Menzel, S. A1 - Bäcker, Matthias A1 - Leinhos, Marcel A1 - Poghossian, Arshak A1 - Wagner, P. A1 - Schöning, Michael Josef T1 - Degradation of thin poly(lactic acid) films: characterization by capacitance-voltage, atomic force microscopy, scanning electron microscopy and contact-angle measurements JF - Electrochimica Acta Y1 - 2013 SN - 1873-3859 (E-Journal); 0013-4686 (Print) VL - Vol. 113 SP - 779 EP - 784 PB - Elsevier CY - Amsterdam ER - TY - CHAP A1 - Huck, Christina A1 - Poghossian, Arshak A1 - Bäcker, Matthias A1 - Zander, W. A1 - Schubert, J. A1 - Sukoyan, L. H. A1 - Begoyan, V. A1 - Buniatyan, V. V. A1 - Wagner, Patrick A1 - Schöning, Michael Josef T1 - Chemische Sensoren mit Bariumstrontiumtitanat als funktionelle Schicht zur Multiparameterdetektion T2 - 11. Dresdner Sensor-Symposium : 9.-11.12.2013 Y1 - 2013 SN - 978-3-9813484-5-3 SP - 368 EP - 372 ER -