TY  - JOUR
A1  - Monakhova, Yulia
A1  - Diehl, Bernd W.K.
T1  - Multinuclear NMR screening of pharmaceuticals using standardization by 2H integral of a deuterated solvent
JF  - Journal of Pharmaceutical and Biomedical Analysis
N2  - NMR standardization approach that uses the 2H integral of deuterated solvent for quantitative multinuclear analysis of pharmaceuticals is described. As a proof of principle, the existing NMR procedure for the analysis of heparin products according to US Pharmacopeia monograph is extended to the determination of Na+ and Cl- content in this matrix. Quantification is performed based on the ratio of a 23Na (35Cl) NMR integral and 2H NMR signal of deuterated solvent, D2O, acquired using the specific spectrometer hardware. As an alternative, the possibility of 133Cs standardization using the addition of Cs2CO3 stock solution is shown. Validation characteristics (linearity, repeatability, sensitivity) are evaluated. A holistic NMR profiling of heparin products can now also be used for the quantitative determination of inorganic compounds in a single analytical run using a single sample. In general, the new standardization methodology provides an appealing alternative for the NMR screening of inorganic and organic components in pharmaceutical products.
KW  - NMR spectroscopy
KW  - Inorganic ions
KW  - Heparin
KW  - Standardization
Y1  - 2022
SN  - 0731-7085
U6  - http://dx.doi.org/10.1016/j.jpba.2021.114530
VL  - 209
IS  - Article number: 114530
PB  - Elsevier
ER  -