TY - JOUR A1 - Uysal, Karya A1 - Firat, Ipek Serat A1 - Creutz, Till A1 - Aydin, Inci Cansu A1 - Artmann, Gerhard A1 - Teusch, Nicole A1 - Temiz Artmann, Aysegül T1 - A novel in vitro wound healing assay using free-standing, ultra-thin PDMS membranes JF - membranes N2 - Advances in polymer science have significantly increased polymer applications in life sciences. We report the use of free-standing, ultra-thin polydimethylsiloxane (PDMS) membranes, called CellDrum, as cell culture substrates for an in vitro wound model. Dermal fibroblast monolayers from 28- and 88-year-old donors were cultured on CellDrums. By using stainless steel balls, circular cell-free areas were created in the cell layer (wounding). Sinusoidal strain of 1 Hz, 5% strain, was applied to membranes for 30 min in 4 sessions. The gap circumference and closure rate of un-stretched samples (controls) and stretched samples were monitored over 4 days to investigate the effects of donor age and mechanical strain on wound closure. A significant decrease in gap circumference and an increase in gap closure rate were observed in trained samples from younger donors and control samples from older donors. In contrast, a significant decrease in gap closure rate and an increase in wound circumference were observed in the trained samples from older donors. Through these results, we propose the model of a cell monolayer on stretchable CellDrums as a practical tool for wound healing research. The combination of biomechanical cell loading in conjunction with analyses such as gene/protein expression seems promising beyond the scope published here. Y1 - 2022 U6 - http://dx.doi.org/10.3390/membranes13010022 N1 - This article belongs to the Special Issue "Latest Scientific Discoveries in Polymer Membranes" VL - 2023 IS - 13(1) PB - MDPI CY - Basel ER - TY - JOUR A1 - Uysal, Karya A1 - Creutz, Till A1 - Firat, Ipek Seda A1 - Artmann, Gerhard A1 - Teusch, Nicole A1 - Temiz Artmann, Aysegül T1 - Bio-functionalized ultra-thin, large-area and waterproof silicone membranes for biomechanical cellular loading and compliance experiments JF - Polymers N2 - Biocompatibility, flexibility and durability make polydimethylsiloxane (PDMS) membranes top candidates in biomedical applications. CellDrum technology uses large area, <10 µm thin membranes as mechanical stress sensors of thin cell layers. For this to be successful, the properties (thickness, temperature, dust, wrinkles, etc.) must be precisely controlled. The following parameters of membrane fabrication by means of the Floating-on-Water (FoW) method were investigated: (1) PDMS volume, (2) ambient temperature, (3) membrane deflection and (4) membrane mechanical compliance. Significant differences were found between all PDMS volumes and thicknesses tested (p < 0.01). They also differed from the calculated values. At room temperatures between 22 and 26 °C, significant differences in average thickness values were found, as well as a continuous decrease in thicknesses within a 4 °C temperature elevation. No correlation was found between the membrane thickness groups (between 3–4 µm) in terms of deflection and compliance. We successfully present a fabrication method for thin bio-functionalized membranes in conjunction with a four-step quality management system. The results highlight the importance of tight regulation of production parameters through quality control. The use of membranes described here could also become the basis for material testing on thin, viscous layers such as polymers, dyes and adhesives, which goes far beyond biological applications. Y1 - 2022 SN - 2073-4360 VL - 14 IS - 11 SP - 2213 PB - MDPI CY - Basel ER - TY - JOUR A1 - Temiz Artmann, Aysegül A1 - Kurulgan demirci, Eylem A1 - Fırat, Ipek Seda A1 - Oflaz, Hakan A1 - Artmann, Gerhard T1 - Recombinant activated protein C (rhAPC) affects lipopolysaccharide-induced mechanical compliance changes and beat frequency of mESC-derived cardiomyocyte monolayers JF - SHOCK KW - Septic cardiomyopathy KW - LPS KW - cardiomyocyte biomechanics KW - CellDrum KW - actin cytoskeleton Y1 - 2021 U6 - http://dx.doi.org/10.1097/SHK.0000000000001845 SN - 1540-0514 PB - Wolters Kluwer CY - Köln ER - TY - RPRT A1 - Temiz Artmann, Aysegül T1 - Frühgeburtenrate mindern durch ein Prognoseverfahren für den vorzeitigen Blasensprung - PROMPT (Premature rupture of membranes prediction test) : Abschlussbericht ; Laufzeit des Vorhabens: 01.03.2007 - 31.12.2009 Y1 - 2010 U6 - http://dx.doi.org/10.2314/GBV:644277858 N1 - Förderkennzeichen BMBF 1772X07 PB - Technische Informationsbibliothek u. Universitätsbibliothek CY - Aachen ER - TY - JOUR A1 - Bayer, Robin A1 - Temiz Artmann, Aysegül A1 - Digel, Ilya A1 - Falkenstein, Julia A1 - Artmann, Gerhard A1 - Creutz, Till A1 - Hescheler, Jürgen T1 - Mechano-pharmacological testing of L-Type Ca²⁺ channel modulators via human vascular celldrum model JF - Cellular Physiology and Biochemistry N2 - Background/Aims: This study aimed to establish a precise and well-defined working model, assessing pharmaceutical effects on vascular smooth muscle cell monolayer in-vitro. It describes various analysis techniques to determine the most suitable to measure the biomechanical impact of vasoactive agents by using CellDrum technology. Methods: The so-called CellDrum technology was applied to analyse the biomechanical properties of confluent human aorta muscle cells (haSMC) in monolayer. The cell generated tensions deviations in the range of a few N/m² are evaluated by the CellDrum technology. This study focuses on the dilative and contractive effects of L-type Ca²⁺ channel agonists and antagonists, respectively. We analyzed the effects of Bay K8644, nifedipine and verapamil. Three different measurement modes were developed and applied to determine the most appropriate analysis technique for the study purpose. These three operation modes are called, particular time mode" (PTM), "long term mode" (LTM) and "real-time mode" (RTM). Results: It was possible to quantify the biomechanical response of haSMCs to the addition of vasoactive agents using CellDrum technology. Due to the supplementation of 100nM Bay K8644, the tension increased approximately 10.6% from initial tension maximum, whereas, the treatment with nifedipine and verapamil caused a significant decrease in cellular tension: 10nM nifedipine decreased the biomechanical stress around 6,5% and 50nM verapamil by 2,8%, compared to the initial tension maximum. Additionally, all tested measurement modes provide similar results while focusing on different analysis parameters. Conclusion: The CellDrum technology allows highly sensitive biomechanical stress measurements of cultured haSMC monolayers. The mechanical stress responses evoked by the application of vasoactive calcium channel modulators were quantified functionally (N/m²). All tested operation modes resulted in equal findings, whereas each mode features operation-related data analysis. Y1 - 2020 U6 - http://dx.doi.org/10.33594/000000225 SN - 1421-9778 VL - 54 SP - 371 EP - 383 PB - Cell Physiol Biochem Press CY - Düsseldorf ER - TY - JOUR A1 - Malik, A. M. A1 - Abdieva, G. Zh. A1 - Ualieva, P. S. A1 - Zhubanova, A. A. A1 - Temiz Artmann, Aysegül T1 - CКPИНИНГ МИКPOOPГAНИЗМOВ-ДECТPУКТOPOВ XЛOРOPГAНИЧECКИX ЗAГPЯЗНИТEЛEЙ T1 - Screening of microorganisms – destructors of chlororganic pollutants JF - Eurasian Journal of Ecology Y1 - 2019 SN - 2617-7358 VL - 61 IS - 4 SP - 61 EP - 71 ER - TY - JOUR A1 - Digel, Ilya A1 - Akimbekov, N. A1 - Turalieva, M. A1 - Mansurov, Z. A1 - Temiz Artmann, Aysegül A1 - Eshibaev, A. A1 - Zhubanova, A. T1 - Usage of Carbonized Plant Wastes for Purification of Aqueous Solutions JF - Journal of Industrial Technology and Engineering Y1 - 2013 VL - 2 IS - 07 SP - 47 EP - 54 ER - TY - JOUR A1 - Akimbekov, N. Sh. A1 - Zhubanova, A. A. A1 - Mansurov, Z. A. A1 - Digel, Ilya A1 - Artmann, Gerhard A1 - Temiz Artmann, Aysegül T1 - Use of Carbonized Rise Shell for the local treatment of wounds JF - Eurasian ChemTech Journal N2 - On the model of musculocutaneous wound in rats, the effect of applicative sorption by carbonized rise shell (CRS) on the healing of festering wound was studied. It has been shown, that cytological changes end with rapid scar formation. The use of CRS at the period of severe purulent wound contributes to its favorable course, prevents the development of complications of the animals from sepsis. Y1 - 2010 U6 - http://dx.doi.org/10.18321/ectj35 SN - 2522-4867 VL - 12 IS - 2 SP - 133 EP - 138 PB - Institute of Combustion Problems CY - Almaty ER - TY - CHAP A1 - Bayer, Robin A1 - Hescheler, Jürgen A1 - Artmann, Gerhard A1 - Temiz Artmann, Aysegül ED - Staat, Manfred ED - Erni, Daniel T1 - Treating arterial hypertension in a cell culture well T2 - 3rd YRA MedTech Symposium 2019 : May 24 / 2019 / FH AachenW N2 - Hypertension describes the pathological increase of blood pressure, which is most commonly associated with the increase of vascular wall stiffness [1]. Referring to the “Deutsche Bluthochdruck Liga” this pathology shows a growing trend in our aging society. In order to find novel pharmacological and probably personalized treatments, we want to present a functional approach to study biomechanical properties of a human aortic vascular model. In this method review we will give an overview of recent studies which were carried out with the CellDrum technology [2] and underline the added value to already existing standard procedures known from the field of physiology. Herein described CellDrum technology is a system to measure functional mechanical properties of cell monolayers and thin tissue constructs in-vitro. Additionally, the CellDrum enables to elucidate the mechanical response of cells to pharmacological drugs, toxins and vasoactive agents. Due to its highly flexible polymer support, cells can also be mechanically stimulated by steady and cyclic biaxial stretching. Y1 - 2019 SN - 978-3-940402-22-6 U6 - http://dx.doi.org/10.17185/duepublico/48750 SP - 5 EP - 6 PB - Universität Duisburg-Essen CY - Duisburg ER - TY - BOOK A1 - Artmann, Gerhard A1 - Temiz Artmann, Aysegül A1 - Zhubanova, Azhar A. A1 - Digel, Ilya ED - Artmann, Gerhard ED - Temiz Artmann, Aysegül ED - Zhubanova, Azhar A. ED - Digel, Ilya T1 - Biological, physical and technical basics of cell engineering Y1 - 2018 SN - 978-981-10-7903-0 PB - Springer CY - Singapore ER -