TY  - JOUR
A1  - Bialonski, Stephan
A1  - Grieger, Niklas
T1  - Der KI-Chatbot ChatGPT: Eine Herausforderung für die Hochschulen
JF  - Die neue Hochschule
N2  - Essays, Gedichte, Programmcode: ChatGPT generiert automatisch Texte auf bisher unerreicht hohem Niveau. Dieses und nachfolgende Systeme werden nicht nur die akademische Welt nachhaltig verändern.
Y1  - 2023
U6  - http://dx.doi.org/10.5281/zenodo.7533758
SN  - 0340-448X
VL  - 2023
IS  - 1
SP  - 24
EP  - 27
PB  - HLB
CY  - Bonn
ER  - 
TY  - JOUR
A1  - Haeger, Gerrit
A1  - Grankin, Alina
A1  - Wagner, Michaela
T1  - Construction of an Aspergillus oryzae triple amylase deletion mutant as a chassis to evaluate industrially relevant amylases using multiplex CRISPR/Cas9 editing technology
JF  - Applied Research
N2  - Aspergillus oryzae is an industrially relevant organism for the secretory production of heterologous enzymes, especially amylases. The activities of potential heterologous amylases, however, cannot be quantified directly from the supernatant due to the high background activity of native α-amylase. This activity is caused by the gene products of amyA, amyB, and amyC. In this study, an in vitro CRISPR/Cas9 system was established in A. oryzae to delete these genes simultaneously. First, pyrG of A. oryzae NSAR1 was mutated by exploiting NHEJ to generate a counter-selection marker. Next, all amylase genes were deleted simultaneously by co-transforming a repair template carrying pyrG of Aspergillus nidulans and flanking sequences of amylase gene loci. The rate of obtained triple knock-outs was 47%. We showed that triple knockouts do not retain any amylase activity in the supernatant. The established in vitro CRISPR/Cas9 system was used to achieve sequence-specific knock-in of target genes. The system was intended to incorporate a single copy of the gene of interest into the desired host for the development of screening methods. Therefore, an integration cassette for the heterologous Fpi amylase was designed to specifically target the amyB locus. The site-specific integration rate of the plasmid was 78%, with exceptional additional integrations. Integration frequency was assessed via qPCR and directly correlated with heterologous amylase activity. Hence, we could compare the efficiency between two different signal peptides. In summary, we present a strategy to exploit CRISPR/Cas9 for gene mutation, multiplex knock-out, and the targeted knock-in of an expression cassette in A. oryzae. Our system provides straightforward strain engineering and paves the way for development of fungal screening systems.
KW  - aspergillus
KW  - CRISPR/Cas9
KW  - filamentous fungi
KW  - genome engineering
Y1  - 2023
U6  - http://dx.doi.org/10.1002/appl.202200106
SN  - 2702-4288
IS  - Early View
SP  - 1
EP  - 15
PB  - Wiley-VCH
ER  - 
TY  - JOUR
A1  - Rübbelke, Dirk
A1  - Vögele, Stefan
A1  - Grajewski, Matthias
A1  - Zobel, Luzy
T1  - Cross border adjustment mechanism: Initial data for the assessment of hydrogen-based steel production
JF  - Data in Brief
N2  - Ambitious climate targets affect the competitiveness of industries in the international market. To prevent such industries from moving to other countries in the wake of increased climate protection efforts, cost adjustments may become necessary. Their design requires knowledge of country-specific production costs. Here, we present country-specific cost figures for different production routes of steel, paying particular attention to transportation costs. The data can be used in floor price models aiming to assess the competitiveness of different steel production routes in different countries (Rübbelke, 2022).
KW  - Energy-intensive industry
KW  - Steel industry
KW  - Competitiveness
KW  - Floor prices
KW  - Cross border adjustment mechanism
Y1  - 2023
U6  - http://dx.doi.org/10.1016/j.dib.2023.108907
SN  - 2352-3409
VL  - 47
IS  - Article 108907
SP  - 1
EP  - 5
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Niedermeier, Jana
A1  - Penner, Crystal
A1  - Usherovich, Samuel
A1  - Bélanger-Champagne, Camille
A1  - Paulßen, Elisabeth
A1  - Cornelia, Hoehr
T1  - Optical Fibers as Dosimeter Detectors for Mixed Proton/Neutron Fields - A Biological Dosimeter
JF  - electronics
N2  - In recent years, proton therapy has gained importance as a cancer treatment modality due to its conformality with the tumor and the sparing of healthy tissue. However, in the interaction of the protons with the beam line elements and patient tissues, potentially harmful secondary neutrons are always generated. To ensure that this neutron dose is as low as possible, treatment plans could be created to also account for and minimize the neutron dose. To monitor such a treatment plan, a compact, easy to use, and inexpensive dosimeter must be developed that not only measures the physical dose, but which can also distinguish between proton and neutron contributions. To that end, plastic optical fibers with scintillation materials (Gd₂O₂S:Tb, Gd₂O₂S:Eu, and YVO₄:Eu) were irradiated with protons and neutrons. It was confirmed that sensors with different scintillation materials have different sensitivities to protons and neutrons. A combination of these three scintillators can be used to build a detector array to create a biological dosimeter.
KW  - biological dosimeter
KW  - Bragg peak
KW  - relative dosimetry
KW  - optical fibers
KW  - proton therapy
KW  - protons
KW  - neutrons
Y1  - 2023
U6  - http://dx.doi.org/10.3390/electronics12020324
SN  - 2079-9292
N1  - This article belongs to the Special Issue "Applications of Optical Fiber Sensors"
VL  - 12
IS  - 2
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Šakić, Bogdan
A1  - Marinković, Marko
A1  - Butenweg, Christoph
A1  - Klinkel, Sven
ED  - Yang, J.
T1  - Influence of slab deflection on the out-of-plane capacity of unreinforced masonry partition walls
JF  - Engineering Structures
N2  - Severe damage of non-structural elements is noticed in previous earthquakes, causing high economic losses and posing a life threat for the people. Masonry partition walls are one of the most commonly used non-structural elements. Therefore, their behaviour under earthquake loading in out-of-plane (OOP) direction is investigated by several researches in the past years. However, none of the existing experimental campaigns or analytical approaches consider the influence of prior slab deflection on OOP response of partition walls. Moreover, none of the existing construction techniques for the connection of partition walls with surrounding reinforced concrete (RC) is investigated for the combined slab deflection and OOP loading. However, the inevitable time-dependent behaviour of RC slabs leads to high values of final slab deflections which can further influence boundary conditions of partition walls. Therefore, a comprehensive study on the influence of slab deflection on the OOP capacity of masonry partitions is conducted. In the first step, experimental tests are carried out. Results of experimental tests are further used for the calibration of the numerical model employed for a parametric study. Based on the results, behaviour under combined loading for different construction techniques is explained. The results show that slab deflection leads either to severe damage or to a high reduction of OOP capacity. Existing practical solutions do not account for these effects. In this contribution, recommendations to overcome the problems of combined slab deflection and OOP loading on masonry partition walls are given. Possible interaction of in-plane (IP) loading, with the combined slab deflection and OOP loading on partition walls, is not investigated in this study.
KW  - Masonry partition walls
KW  - Earthquake
KW  - Out-of-plane capacity
KW  - Slab deflection
Y1  - 2023
U6  - http://dx.doi.org/10.1016/j.engstruct.2022.115342
SN  - 0141-0296
VL  - 276
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - BOOK
A1  - Drumm, Christian
A1  - Scheuermann, Bernd
A1  - Weidner, Stefan
T1  - Einstieg in SAP S/4HANA® : Geschäftsprozesse, Anwendungen, Zusammenhänge – Erklärt am Beispielunternehmen Global Bike
N2  - Dieser verständliche Einstieg in SAP S/4HANA führt Sie anhand des Beispielunternehmens Global Bike durch die zentralen Abläufe in Vertrieb, Einkauf, Rechnungswesen, Produktion und Lagerverwaltung. Sie werden mit den betriebswirtschaftlichen Grundlagen, den relevanten Organisationsstrukturen und Stammdaten sowie den Prozessen vertraut gemacht. Mithilfe von Praxisbeispielen und Fallstudien sind Sie schon bald SAP-S/4HANA-Profi – für mehr Erfolg in Studium und Beruf!
Y1  - 2023
SN  - 9783836281560 (Print)
SN  - 9783836281584 (E-Book)
N1  - Unter der Signatur 21 QGT 220 in der Bibliothek Eupener Straße vorhanden.
PB  - Rheinwerk Verlag
CY  - Bonn
ER  - 
TY  - CHAP
A1  - Chircu, Alina
A1  - Czarnecki, Christian
A1  - Friedmann, Daniel
A1  - Pomaskow, Johanna
A1  - Sultanow, Eldar
T1  - Towards a Digital Twin of Society
T2  - Proceedings of the 56th Hawaii International Conference on System Sciences 2023
N2  - This paper describes the potential for developing a digital twin of society- a dynamic model that can be used to observe, analyze, and predict the evolution of various societal aspects. Such a digital twin can help governmental agencies and policy makers in interpreting trends, understanding challenges, and making decisions regarding investments or policies necessary to support societal development and ensure future prosperity. The paper reviews related work regarding the digital twin paradigm and its applications. The paper presents a motivating case study- an analysis of opportunities and challenges faced by the German federal employment agency, Bundesagentur f¨ur Arbeit (BA), proposes solutions using digital twins, and describes initial proofs of concept for such solutions.
KW  - Digital twin
KW  - Digital transformation
KW  - Prototype
KW  - Society
KW  - Stress testing
Y1  - 2023
SN  - 978-0-9981331-6-4
SP  - 6748
EP  - 6757
PB  - University of Hawai'i
CY  - Honolulu
ER  -