TY - JOUR A1 - Bassam, Rasha A1 - Hescheler, Jürgen A1 - Temiz Artmann, Aysegül A1 - Artmann, Gerhard A1 - Digel, Ilya T1 - Effects of spermine NONOate and ATP on the thermal stability of hemoglobin JF - BMC Biophysics N2 - Background Minor changes in protein structure induced by small organic and inorganic molecules can result in significant metabolic effects. The effects can be even more profound if the molecular players are chemically active and present in the cell in considerable amounts. The aim of our study was to investigate effects of a nitric oxide donor (spermine NONOate), ATP and sodium/potassium environment on the dynamics of thermal unfolding of human hemoglobin (Hb). The effect of these molecules was examined by means of circular dichroism spectrometry (CD) in the temperature range between 25°C and 70°C. The alpha-helical content of buffered hemoglobin samples (0.1 mg/ml) was estimated via ellipticity change measurements at a heating rate of 1°C/min. Results Major results were: 1) spermine NONOate persistently decreased the hemoglobin unfolding temperature T u irrespectively of the Na + /K + environment, 2) ATP instead increased the unfolding temperature by 3°C in both sodium-based and potassium-based buffers and 3) mutual effects of ATP and NO were strongly influenced by particular buffer ionic compositions. Moreover, the presence of potassium facilitated a partial unfolding of alpha-helical structures even at room temperature. Conclusion The obtained data might shed more light on molecular mechanisms and biophysics involved in the regulation of protein activity by small solutes in the cell. KW - Nitric Oxide Donor KW - NONOate KW - Circular Dichroism KW - Nitric Oxide Y1 - 2012 U6 - http://dx.doi.org/10.1186/2046-1682-5-16 SN - 2046-1682 VL - 5 PB - BioMed Central CY - London ER - TY - JOUR A1 - Preiß, C. A1 - Linder, Peter A1 - Wendt, K. A1 - Krystek, M. A1 - Digel, Ilya A1 - Gossmann, Matthias A1 - Temiz Artmann, Aysegül A1 - Porst, Dariusz A1 - Kayser, Peter A1 - Bassam, Rasha A1 - Artmann, Gerhard T1 - Engineering technology for plant physiology and plant stress research N2 - Plant physiology and plant stress: Plant physiology will be much more important for human mankind because of yield and cultivation limits of crops determined by their resistance to stress. To assess and counteract various stress factors it is necessary to conduct plant research to gain information and results on plant physiology. KW - Pflanzenphysiologie KW - Pflanzenstress KW - Pflanzenscanner KW - plant stress KW - plant scanner Y1 - 2011 ER - TY - JOUR A1 - Artmann, Gerhard A1 - Hovav, T. A1 - Goldfarb, A. A1 - Yedgar, S. [u.a.] T1 - Enhanced adherence of beta-thalassaemic erythrocytes to endothelial cells. Hovav, T., Goldfarb, A.; Artmann, Gerhard Michael, Yedgar, S.; Barstein, G. JF - British Journal of Haematology. 106 (1999), H. 1 Y1 - 1999 SN - 1365-2141 SP - 178 EP - 181 ER - TY - JOUR A1 - Artmann, Gerhard A1 - Vogel, A. A1 - Zink, S. A1 - Stache, O. T1 - Entwicklung eines automatisierten, computergesteuerten Permeabilitätsanalysators zur Messung an Endothelzellen / Vogel, A. ; Zinke, S. ; Stache, O. ; Königsfeld, J. ; Artmann, G. JF - Biomedizinische Technik = Biomedical Engineering. 45 (2000), H. s1 Y1 - 2000 SN - 1862-278X SP - 479 EP - 480 ER - TY - JOUR A1 - Trzewik, Jürgen A1 - Temiz Artmann, Aysegül A1 - Linder, Peter A1 - Demirci, T. A1 - Digel, Ilya A1 - Artmann, Gerhard T1 - Evaluation of lateral mechanical tension in thin-film tissue constructs JF - Annals of Biomedical Engineering. 32 (2004), H. 9 Y1 - 2004 SN - 1573-9686 SP - 1243 EP - 1251 ER - TY - JOUR A1 - Artmann, Gerhard A1 - Trzewik, Jürgen A1 - Mallipattu, S. K. A1 - Delano, F. A. [u.a.] T1 - Evidence for a second valve system in Lymphatics: Endothelial Microvalves. Trzewik, Jürgen; Mallipattu, S. K.; Artmann, Gerhard Michael; Delano, F. A.; Schmid-Schonbein, G. W JF - The FASEB Journal. 15 (2001) Y1 - 2001 SN - 1530-6860 SP - 1711 EP - 1717 ER - TY - JOUR A1 - Artmann, Gerhard A1 - Witte, Ch. T1 - Expertensystem für Personal Computer zur Strukturbestimmung des Circulus Arteriosus Willisii mittels transkranieller Dopplersonografie. Witte, Ch.; Artmann, Gerhard Michael JF - Biomedizinische Technik = Biomedical engineering. 36 (1991) Y1 - 1991 SN - 0013-5585 SP - 228 EP - 230 ER - TY - JOUR A1 - Leimena, W. A1 - Artmann, Gerhard A1 - Dachwald, Bernd A1 - Temiz Artmann, Aysegül A1 - Gossmann, Matthias A1 - Digel, Ilya T1 - Feasibility of an in-situ microbial decontamination of an ice-melting probe JF - Eurasian Chemico-Technological Journal. 12 (2010), H. 2 Y1 - 2010 SN - 1562-3920 SP - 145 EP - 150 ER - TY - JOUR A1 - Artmann, Gerhard A1 - Hueck, Isgard S. A1 - Rossiter, Katharine A1 - Schmid-Schönbein, Geert W. T1 - Fluid Shear Attenuates Endothelial Pseudopodia Formation into the Capillary Lumen / Hueck, Isgard S. ; Rossiter, Katharine ; Artman, Gerhard M. ; Schmid-Schönbein, Geert W. JF - Microcirculation. 15 (2008), H. 6 Y1 - 2008 SN - 1549-8719 SP - 531 EP - 542 ER - TY - JOUR A1 - Stadler, Andreas M. A1 - Digel, Ilya A1 - Embs, Jan P. A1 - Unruh, Tobias A1 - Tehei, M. A1 - Zaccai, G. A1 - Büldt, G. A1 - Artmann, Gerhard T1 - From powder to solution : Hydration dependence of human hemoglobin dynamics correlated to body temperature JF - Biophysical Journal. 96 (2009), H. 12 Y1 - 2009 SN - 0006-3495 SP - 5073 EP - 5081 PB - Cell Press CY - Cambridge, Mass. ER -