TY - JOUR A1 - Falkenberg, Fabian A1 - Voß, Leonie A1 - Bott, Michael A1 - Bongaerts, Johannes A1 - Siegert, Petra T1 - New robust subtilisins from halotolerant and halophilic Bacillaceae JF - Applied Microbiology and Biotechnology N2 - The aim of the present study was the characterisation of three true subtilisins and one phylogenetically intermediate subtilisin from halotolerant and halophilic microorganisms. Considering the currently growing enzyme market for efficient and novel biocatalysts, data mining is a promising source for novel, as yet uncharacterised enzymes, especially from halophilic or halotolerant Bacillaceae, which offer great potential to meet industrial needs. Both halophilic bacteria Pontibacillus marinus DSM 16465ᵀ and Alkalibacillus haloalkaliphilus DSM 5271ᵀ and both halotolerant bacteria Metabacillus indicus DSM 16189 and Litchfieldia alkalitelluris DSM 16976ᵀ served as a source for the four new subtilisins SPPM, SPAH, SPMI and SPLA. The protease genes were cloned and expressed in Bacillus subtilis DB104. Purification to apparent homogeneity was achieved by ethanol precipitation, desalting and ion-exchange chromatography. Enzyme activity could be observed between pH 5.0–12.0 with an optimum for SPPM, SPMI and SPLA around pH 9.0 and for SPAH at pH 10.0. The optimal temperature for SPMI and SPLA was 70 °C and for SPPM and SPAH 55 °C and 50 °C, respectively. All proteases showed high stability towards 5% (w/v) SDS and were active even at NaCl concentrations of 5 M. The four proteases demonstrate potential for future biotechnological applications. KW - Biotechnological application KW - Bacillaceae KW - Subtilisin KW - Subtilases KW - Halotolerant protease Y1 - 2023 U6 - https://doi.org/10.1007/s00253-023-12553-w SN - 1432-0614 N1 - Corresponding author: Petra Siegert VL - 107 SP - 3939 EP - 3954 PB - Springer Nature CY - Berlin ER - TY - CHAP A1 - Gellert, Christoph A1 - Norda, Hannah A1 - Butenweg, Christoph T1 - Nonlinear behaviour of masonry under cyclic loading T2 - 7th European Conference on Structural Dynamics, EURODYN 2008 : 7-9 July 2008, Southampton Y1 - 2008 SP - 1 EP - 12 ER - TY - JOUR A1 - Haeger, Gerrit A1 - Probst, Johanna A1 - Jaeger, Karl-Erich A1 - Bongaerts, Johannes A1 - Siegert, Petra T1 - Novel aminoacylases from Streptomyces griseus DSM 40236 and their recombinant production in Streptomyces lividans JF - FEBS Open Bio N2 - Amino acid-based surfactants are valuable compounds for cosmetic formulations. The chemical synthesis of acyl-amino acids is conventionally performed by the Schotten-Baumann reaction using fatty acyl chlorides, but aminoacylases have also been investigated for use in biocatalytic synthesis with free fatty acids. Aminoacylases and their properties are diverse; they belong to different peptidase families and show differences in substrate specificity and biocatalytic potential. Bacterial aminoacylases capable of synthesis have been isolated from Burkholderia, Mycolicibacterium, and Streptomyces. Although several proteases and peptidases from S. griseus have been described, no aminoacylases from this species have been identified yet. In this study, we investigated two novel enzymes produced by S. griseus DSM 40236ᵀ . We identified and cloned the respective genes and recombinantly expressed an α-aminoacylase (EC 3.5.1.14), designated SgAA, and an ε-lysine acylase (EC 3.5.1.17), designated SgELA, in S. lividans TK23. The purified aminoacylase SgAA was biochemically characterized, focusing on its hydrolytic activity to determine temperature- and pH optima and stabilities. The aminoacylase could hydrolyze various acetyl-amino acids at the Nα -position with a broad specificity regarding the sidechain. Substrates with longer acyl chains, like lauroyl-amino acids, were hydrolyzed to a lesser extent. Purified aminoacylase SgELA specific for the hydrolysis of Nε -acetyl-L-lysine was unstable and lost its enzymatic activity upon storage for a longer period but could initially be characterized. The pH optimum of SgELA was pH 8.0. While synthesis of acyl-amino acids was not observed with SgELA, SgAA catalyzed the synthesis of lauroyl-methionine. KW - Streptomyces lividans KW - recombinant expression KW - Streptomyces griseus KW - ε-lysine acylase KW - α-aminoacylase Y1 - 2023 U6 - https://doi.org/10.1002/2211-5463.13723 SN - 2211-5463 N1 - Corresponding author: Petra Siegert VL - 13 IS - 12 SP - 2224 EP - 2238 PB - Wiley CY - Hoboken, NJ ER - TY - CHAP A1 - Arida, Hassan A. A1 - Kloock, Joachim P. A1 - Schöning, Michael Josef T1 - Novel organic membrane-based thin-film microsensors for the determination of heavy metal cations N2 - A first step towards the fabrication and electrochemical evaluation of thin-film microsensors based on organic PVC membranes for the determination of Hg(II), Cd(II), Pb(II) and Cu(II) ions in solutions has been realised. The membrane-coating mixture used in the preparation of this new type of microsensors is incorporating PVC as supporting matrix, o-nitrophenyloctylether (o-NPOE) as solvent mediator and a recently synthesized Hg[dimethylglyoxime(phene)]2+ and Bis-(4-hydroxyacetophenone)-ethylenediamine as electroactive materials for Hg(II) and Cd(II), respectively. A set of three commercialised ionophores for Cd(II), Pb(II) and Cu(II) has been also used for comparison. Thin-film microsensors based on these membranes showed a Nernstian response of slope (26-30 mV/dec.) for the respective tested cations. The potentiometric response characteristics (linear range, pH range, detection limit and response time) are comparable with those obtained by conventional membranes as well as coated wire electrodes prepared from the same membrane. The realisation of the new organic membrane-based thin-film microsensors overcomes the problem of an insufficient selectivity of solid-state-based thinfilm sensors. KW - Biosensor KW - Heavy metal detection KW - thin-film microsensors KW - organic PVC membranes Y1 - 2006 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:hbz:a96-opus-1545 ER - TY - JOUR A1 - Haeger, Gerrit A1 - Jolmes, Tristan A1 - Oyen, Sven A1 - Jaeger, Karl-Erich A1 - Bongaerts, Johannes A1 - Schörken, Ulrich A1 - Siegert, Petra T1 - Novel recombinant aminoacylase from Paraburkholderia monticola capable of N-acyl-amino acid synthesis JF - Applied Microbiology and Biotechnology N2 - N-Acyl-amino acids can act as mild biobased surfactants, which are used, e.g., in baby shampoos. However, their chemical synthesis needs acyl chlorides and does not meet sustainability criteria. Thus, the identification of biocatalysts to develop greener synthesis routes is desirable. We describe a novel aminoacylase from Paraburkholderia monticola DSM 100849 (PmAcy) which was identified, cloned, and evaluated for its N-acyl-amino acid synthesis potential. Soluble protein was obtained by expression in lactose autoinduction medium and co-expression of molecular chaperones GroEL/S. Strep-tag affinity purification enriched the enzyme 16-fold and yielded 15 mg pure enzyme from 100 mL of culture. Biochemical characterization revealed that PmAcy possesses beneficial traits for industrial application like high temperature and pH-stability. A heat activation of PmAcy was observed upon incubation at temperatures up to 80 °C. Hydrolytic activity of PmAcy was detected with several N-acyl-amino acids as substrates and exhibited the highest conversion rate of 773 U/mg with N-lauroyl-L-alanine at 75 °C. The enzyme preferred long-chain acyl-amino-acids and displayed hardly any activity with acetyl-amino acids. PmAcy was also capable of N-acyl-amino acid synthesis with good conversion rates. The best synthesis results were obtained with the cationic L-amino acids L-arginine and L-lysine as well as with L-leucine and L-phenylalanine. Exemplarily, L-phenylalanine was acylated with fatty acids of chain lengths from C8 to C18 with conversion rates of up to 75%. N-lauroyl-L-phenylalanine was purified by precipitation, and the structure of the reaction product was verified by LC–MS and NMR. KW - Chaperone KW - Biocatalysis KW - Aminoacylase KW - Acylation KW - Acyl-amino acids KW - Biosurfactants Y1 - 2024 U6 - https://doi.org/10.1007/s00253-023-12868-8 SN - 1432-0614 N1 - Corresponding author: Petra Siegert IS - 108 PB - Springer CY - Berlin ER - TY - RPRT A1 - Weßling, Matthias T1 - O Perfil Reiss: Conceito scientifico e conclusões da pesquisa, aplicações exemplaricas e conseqüências práticas T1 - The Reiss-Profile: scientific concept and conclusions of research results, exemplarily applications and practical consequences N2 - Para Reiss, a avaliação de cada um dos 16 motivos vitais é a chave para não só entender o comportamento humano, como também para poder prevê-lo. Tal previsão é também a meta de cada ciência. Quando se quer saber a reação das pessoas, precisa-se primeiro descobrir, o que as pessoas realmente querem – e, então prever, que elas satisfarão esses desejos e necessidades em suas ações. As chances, que o Perfil Reiss oferece, resultam entre outras também, que aqui é possível pela primeira vez uma individualização total. Cerca de 6 bilhões de perfis individuais podem ser apresentados com o Perfil Reiss. Na combinação correta da formação e utilização da tipologia de um lado e da individualidade da conclusão de outro lado, está a força positiva desse instrumento. N2 - Das Reiss-Profil der 16 Lebensmotive ist Ergebnis einer umfassenden, länderübergreifenden empirischen Datenerhebung und beruht auf einer bislang einzigartigen empirischen Forschungsbasis. Es überprüft die Kongruenz zwischen dem Anforderungsprofil eines Stelle und dem Motivationsprofil eines Menschen. Das Reiss-Profil lässt bewusste und unbewusste Motive und Antriebe von Mitarbeitern erkennen, die neue Management- Firmeninitiativen blockieren oder fördern können. Bereits im Vorfeld werden für den einzelnen Mitarbeiter außerordentlich wichtige Werte anhand ihres Motivprofils erkannt und können besser in die Gruppe integriert werden. Im Endergebnis verdeutlicht diese Perspektive für die betriebliche Mitarbeitermotvation eine vollkommene Individualisierung, wenngleich das Ausmaß auch kulturabhängig relativiert werden muß. KW - Motivation KW - Führung KW - Führung KW - Motivation KW - Leadership KW - Motivation KW - Self-Leadership Y1 - 2006 ER - TY - THES A1 - Vu, Tuan Dat T1 - Objekterkennung und Schienenerkennung in der Schienenfahrzeugtechnik Y1 - 2022 PB - FH Aachen CY - Aachen ER - TY - CHAP A1 - Bung, Daniel Bernhard T1 - Observations on non-aerated flow and air entrainment on moderately sloped stepped spillways T2 - 7th Chinese-German Joint Symposium on Hydraulic and Ocean Engineering : 8. - 12. Sept. 2014, Hannover Y1 - 2014 N1 - Kennwortgeschützter Zugang. Passwort bei Prof. Bung, Fachbereich Bauingenieurwesen SP - 1 EP - 8 ER - TY - JOUR A1 - Möhren, Felix A1 - Bergmann, Ole A1 - Janser, Frank A1 - Braun, Carsten T1 - On the influence of elasticity on propeller performance: a parametric study JF - CEAS Aeronautical Journal N2 - The aerodynamic performance of propellers strongly depends on their geometry and, consequently, on aeroelastic deformations. Knowledge of the extent of the impact is crucial for overall aircraft performance. An integrated simulation environment for steady aeroelastic propeller simulations is presented. The simulation environment is applied to determine the impact of elastic deformations on the aerodynamic propeller performance. The aerodynamic module includes a blade element momentum approach to calculate aerodynamic loads. The structural module is based on finite beam elements, according to Timoshenko theory, including moderate deflections. Several fixed-pitch propellers with thin-walled cross sections made of both isotropic and non-isotropic materials are investigated. The essential parameters are varied: diameter, disc loading, sweep, material, rotational, and flight velocity. The relative change of thrust between rigid and elastic blades quantifies the impact of propeller elasticity. Swept propellers of large diameters or low disc loadings can decrease the thrust significantly. High flight velocities and low material stiffness amplify this tendency. Performance calculations without consideration of propeller elasticity can lead to decreased efficiency. To avoid cost- and time-intense redesigns, propeller elasticity should be considered for swept planforms and low disc loadings. KW - Propeller KW - Finite element method KW - Blade element method KW - Propeller elasticity KW - Aeroelasticity Y1 - 2023 U6 - https://doi.org/10.1007/s13272-023-00649-y SN - 1869-5590 (Online) SN - 1869-5582 (Print) N1 - Corresponding author: Felix Möhren VL - 14 SP - 311 EP - 323 PB - Springer Nature CY - Berlin ER - TY - THES A1 - Kauffmann, Michael T1 - OpenCouch : Therapiesuche leicht gemacht. N2 - Die Auswirkungen psychischer Erkrankungen stellen ein zunehmendes Thema in unserer Gesellschaft dar. Allein in Deutschland erkranken jedes Jahr ein Viertel aller Menschen psychisch. Besonders seit der Pandemie wächst die Nachfrage nach psychotherapeutischer Behandlung stetig an, wodurch die Wartelisten in den Praxen immer länger werden. Betroffene müssen oft ein halbes Jahr oder länger auf einen Therapieplatz warten und sind gezwungen zu nehmen, was gerade frei ist. Dabei ist es für den Therapieerfolg essenziell, dass nicht nur die Therapie auf einen abgestimmt ist, sondern auch die Chemie zwischen Therapeut:in und Patient:in passt. OpenCouch ist eine Anwendung, die Betroffene bei der Therapieplatzsuche unterstützen soll, indem sie Informationen und Tools zur Verfügung stellt, um trotz der geringen Verfügbarkeit einen Therapieplatz finden zu können, der einen Behandlungserfolg garantiert. KW - Psychotherapie KW - Therapieplatzsuche KW - Therapeutensuche KW - Mental Health KW - UX-Design Y1 - 2023 PB - FH Aachen CY - Aachen ER - TY - JOUR A1 - Cheenakula, Dheeraja A1 - Paulsen, Svea A1 - Ott, Fabian A1 - Grömping, Markus T1 - Operational window of a deammonifying sludge for mainstream application in a municipal wastewater treatment plant JF - Water and Environment Journal N2 - The present work aimed to study the mainstream feasibility of the deammonifying sludge of side stream of municipal wastewater treatment plant (MWWTP) in Kaster, Germany. For this purpose, the deammonifying sludge available at the side stream was investigated for nitrogen (N) removal with respect to the operational factors temperature (15–30°C), pH value (6.0–8.0) and chemical oxygen demand (COD)/N ratio (≤1.5–6.0). The highest and lowest N-removal rates of 0.13 and 0.045 kg/(m³ d) are achieved at 30 and 15°C, respectively. Different conditions of pH and COD/N ratios in the SBRs of Partial nitritation/anammox (PN/A) significantly influenced both the metabolic processes and associated N-removal rates. The scientific insights gained from the current work signifies the possibility of mainstream PN/A at WWTPs. The current study forms a solid basis of operational window for the upcoming semi-technical trails to be conducted prior to the full-scale mainstream PN/A at WWTP Kaster and WWTPs globally. KW - Anammox KW - Mainstream KW - Nitrogen removal KW - Partial nitritation KW - Wastewater Y1 - 2023 U6 - https://doi.org/10.1111/wej.12898 SN - 1747-6593 N1 - Corresponding author: Dheeraja Cheenakula VL - 38 IS - 1 SP - 59 EP - 70 PB - Wiley CY - Chichester ER - TY - CHAP A1 - Kraft, Bodo A1 - Retkowitz, Daniel T1 - Operationale Semantikdefinition für konzeptuelles Regelwissen N2 - In: Forum Bauinformatik 2005 : junge Wissenschaftler forschen / [Lehrstuhl Bauinformatik, Brandenburgische Technische Universität Cottbus. Frank Schley ... (Hrsg.)]. - Cottbus : Techn. Universität 2005. S. 1-10 ISBN 3-934934-11-0 Mittels eines operationalen Ansatzes zur Semantikdefinition wird am Bei-spiel des konzeptuellen Gebäudeentwurfs ein Regelsystem formalisiert. Dazu werdenzwei Teile, zum einen das Regelwissen, zum anderen ein konzeptueller Entwurfsplan zunächst informell eingeführt und dann formal beschrieben. Darauf aufbauend wird die Grundlage für eine Konsistenzprüfung des konzeptuellen Entwurfs gegen das Regel-wissen formal angeben KW - CAD KW - CAD KW - Bauingenieurwesen KW - CAD KW - civil engineering Y1 - 2005 ER - TY - GEN A1 - Bellenberg, Markus T1 - Optimierung einer Roboter-Schweißzelle als Basis eines E-Learning Systems T1 - Optimization of a Robot-welding Station as the Basis for an E-Learning System N2 - Ziel war die technische Überarbeitung und Optimierung des vorliegenden Prototypen, um eine Basis für ein E-Learning System (Internet unterstütztes Lernen) zu schaffen. Zu diesem Zweck wurde das Programm der Robotersteuerung neu erstellt, notwendige mechanische Änderungen an Roboterequipment und Materialförderung vorgenommen sowie eine neue Internet-Anbindung geschaffen. Wesentliche Ergebnisse der Arbeit sind: • Erzeugen roboterbasierter kreisinterpolierter Bahnschweißnähte • Kommunikation des Lernsystems via Internet • Lernorientierte Steuerungssoftware N2 - The main goal of this final thesis was the revision and optimization of the given prototype as a basis for an E-Learning environment. All components of the system, including specific technical aspects, are discussed in order to create the basis for future training documentation. Similarly, the programs for the robot motion sequences and welding processes were renewed in order to create well-structured, clear and properly documented software that is suitable for use in a formal training program. KW - Roboter KW - MAG-Schweißen KW - MIG-Schweißen KW - Schweißen KW - Mechatronik KW - Kawasaki KW - E-Learning KW - Roboter-Schweißen KW - welding KW - welded joint KW - mechatronics Y1 - 2002 ER - TY - CHAP A1 - Jung, Alexander A1 - Staat, Manfred A1 - Müller, Wolfram ED - Onate, E. T1 - Optimization of the flight style in ski jumping T2 - 11th World Congress on Computational Mechanics (WCCM XI) ; 5th European Conference on Computational Mechanics (ECCM V) ; 6th European Conference on Computational Fluid Dynamics (ECFD VI) ; July 20 - 25, 2014, Barcelona Y1 - 2014 N1 - Das Paper wurde nach der Konferenz überarbeitet. SP - 799 EP - 810 ER - TY - GEN T1 - Ordnung zur Aufhebung der Prüfungsordnung für den Masterstudiengang „Elektrotechnik“ (zuvor „Nachrichtentechnik“ und „Elektrotechnik und Informationstechnik“) vom 22. Januar 2010 (FH-Mitteilung Nr. 7/2010), zuletzt geändert durch Änderungsordnung vom 25. April 2016 (FH-Mitteilung Nr. 31/2016) : vom 20. Dezember 2019 T3 - FH-Mitteilungen - 134/2019 KW - Amtliche Mitteilung KW - Aufhebungsordnung KW - Prüfungsordnung KW - Master KW - Elektrotechnik Y1 - 2019 ER - TY - GEN T1 - Ordnung zur Aufhebung der Prüfungsordnung für den Masterstudiengang „Information Systems Engineering“ vom 22. Januar 2010 (FH-Mitteilung Nr. 5/2010), zuletzt geändert durch Änderungsordnung vom 4. Februar 2013 (FH-Mitteilung Nr. 6/2013) : vom 20. Dezember 2019 T3 - FH-Mitteilungen - 133/2019 KW - Amtliche Mitteilung KW - Aufhebungsordnung KW - Prüfungsordnung KW - Master KW - Information Systems Engineering Y1 - 2019 ER - TY - GEN T1 - Ordnung zur Aufhebung der Prüfungsordnung für die Masterstudiengänge „Energiewirtschaft & Informatik“ (3 Semester) und „Energiewirtschaft & Informatik“ (4 Semester) vom 4. Mai 2016 (FH-Mitteilung Nr. 59/2016) Fachbereich Energietechnik Fachbereich Medizintechnik und Technomathematik an der Fachhochschule Aachen : vom 16. Januar 2020 T3 - FH-Mitteilungen - 1/2020 KW - Amtliche Mitteilung KW - Aufhebungsordnung KW - Prüfungsordnung KW - Master KW - Energiewirtschaft & Informatik Y1 - 2020 ER - TY - GEN T1 - Ordnung zur Aufhebung der Prüfungsordnungen für den Masterstudiengang „Nuclear Applications“ vom 13. Februar 2004 (FH-Mitteilung Nr. 5/2004) und vom 25. Februar 2010 (FH-Mitteilung Nr. 15/2010) in den Fassungen der Bekanntmachung der Änderungsordnungen vom 6. November 2012 (FH-Mitteilung Nr. 120/2012), 18. Juni 2014 (FH- Mitteilung Nr. 72/2014) und vom 15. Juni 2015 (FH-Mitteilung Nr. 28/2015) : vom 29. November 2019 T3 - FH-Mitteilungen - 127/2019 KW - Amtliche Mitteilung KW - Aufhebungsordnung KW - Prüfungsordnung KW - Master KW - Nuclear Applications Y1 - 2023 ER - TY - GEN T1 - Ordnung zur Aufhebung der Studien- und Prüfungsordnung für den Bachelorstudiengang „Holzingenieurwesen“ (7-semestrig) vom 16. Juli 2012 (FH-Mitteilung Nr. 66/2012) in der Fassung der Bekanntmachung der Änderungsordnungen vom 6. Juli 2016 (FH-Mitteilung Nr. 95/2016) und 16. August 2019 (FH-Mitteilung Nr. 76/2019) an der Fachhochschule Aachen : vom 24. Juli 2018 – FH-Mitteilung Nr. 120/2018 in der Fassung der Bekanntmachung der Änderungsordnung vom 16. August 2019 – FH-Mitteilung Nr. 77/2019 (Nichtamtliche lesbare Fassung) T3 - FH-Mitteilungen - 88/2019 KW - Amtliche Mitteilung KW - Lesbare Fassung KW - Studien- und Prüfungsordnung KW - Bachelor KW - Holzingenieurwesen Y1 - 2019 ER - TY - GEN T1 - Ordnung zur Aufhebung der Studien- und Prüfungsordnung für den Masterstudiengang „Bauingenieurwesen“ vom 10. Dezember 2012 (FH-Mitteilung Nr. 129/2012) in der Fassung der Bekanntmachung der Änderungsordnung vom 30. Mai 2017 (FH-Mitteilung Nr. 58/2017) und 16. August 2019 (FH-Mitteilung Nr. 80/2019) an der Fachhochschule Aachen : vom 24. Juli 2018 – FH-Mitteilung Nr. 121/2018 in der Fassung der Bekanntmachung der Änderungsordnung vom 16. August 2019 – FH-Mitteilung Nr. 81/2019 (Nichtamtliche lesbare Fassung) T3 - FH-Mitteilungen - 94/2019 KW - Amtliche Mitteilung KW - Lesbare Fassung KW - Studien- und Prüfungsordnung KW - Master KW - Bauingenieurwesen Y1 - 2019 ER -