TY  - JOUR
A1  - Eggert, Mathias
A1  - Kling, Rene
T1  - How to distribute charging requests of electronic vehicles? A reservation-based approach
JF  - International Journal of Intelligent Transportation Systems Research
N2  - The number of electronic vehicles increase steadily while the space for extending the charging infrastructure is limited. In particular in urban areas, where parking spaces in attractive areas are famous, opportunities to setup new charging stations is very limited. This leads to an overload of some very attractive charging stations and an underutilization of less attractive ones. Against this background, the paper at hand presents the design of an e-vehicle reservation system that aims at distributing the utilization of the charging infrastructure, particularly in urban areas. By applying a design science approach, the requirements for a reservation-based utilization approach are elicited and a model for a suitable distribution approach and its instantiation are developed. The artefact is evaluated by simulating the distribution effects based on data of real charging station utilizations.
KW  - Simulation
KW  - Parking
KW  - Charging station
KW  - Utilization improvement
KW  - Reservation system
KW  - Electronic vehicle
Y1  - 2023
U6  - http://dx.doi.org/10.1007/s13177-023-00367-z
SN  - 1868-8659
N1  - Corresponding author: Mathias Eggert
VL  - 21
IS  - 2023
SP  - 437
EP  - 460
PB  - Springer
CY  - Berlin, Heidelberg, New York
ER  - 
TY  - JOUR
A1  - Wendlandt, Tim
A1  - Koch, Claudia
A1  - Britz, Beate
A1  - Liedek, Anke
A1  - Schmidt, Nora
A1  - Werner, Stefan
A1  - Gleba, Yuri
A1  - Vahidpour, Farnoosh
A1  - Welden, Melanie
A1  - Poghossian, Arshak
A1  - Schöning, Michael Josef
T1  - Facile Purification and Use of Tobamoviral Nanocarriers for Antibody-Mediated Display of a Two-Enzyme System
JF  - Viruses
N2  - Immunosorbent turnip vein clearing virus (TVCV) particles displaying the IgG-binding domains D and E of Staphylococcus aureus protein A (PA) on every coat protein (CP) subunit (TVCVPA) were purified from plants via optimized and new protocols. The latter used polyethylene glycol (PEG) raw precipitates, from which virions were selectively re-solubilized in reverse PEG concentration gradients. This procedure improved the integrity of both TVCVPA and the wild-type subgroup 3 tobamovirus. TVCVPA could be loaded with more than 500 IgGs per virion, which mediated the immunocapture of fluorescent dyes, GFP, and active enzymes. Bi-enzyme ensembles of cooperating glucose oxidase and horseradish peroxidase were tethered together on the TVCVPA carriers via a single antibody type, with one enzyme conjugated chemically to its Fc region, and the other one bound as a target, yielding synthetic multi-enzyme complexes. In microtiter plates, the TVCVPA-displayed sugar-sensing system possessed a considerably increased reusability upon repeated testing, compared to the IgG-bound enzyme pair in the absence of the virus. A high coverage of the viral adapters was also achieved on Ta2O5 sensor chip surfaces coated with a polyelectrolyte interlayer, as a prerequisite for durable TVCVPA-assisted electrochemical biosensing via modularly IgG-assembled sensor enzymes.
KW  - biosensor
KW  - horseradish peroxidase (HRP)
KW  - glucose oxidase (GOx)
KW  - enzyme cascade
KW  - turnip vein clearing virus (TVCV)
KW  - tobacco mosaic virus (TMV)
Y1  - 2023
U6  - http://dx.doi.org/doi.org/10.3390/v15091951
SN  - 1999-4915
N1  - This article belongs to the Special Issue "Tobamoviruses 2023"
VL  - 9
IS  - 15
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Bertz, Morten
A1  - Molinnus, Denise
A1  - Schöning, Michael Josef
A1  - Homma, Takayuki
T1  - Real-time monitoring of H₂O₂ sterilization on individual bacillus atrophaeus spores by optical sensing with trapping Raman spectroscopy
JF  - Chemosensors
N2  - Hydrogen peroxide (H₂O₂), a strong oxidizer, is a commonly used sterilization agent employed during aseptic food processing and medical applications. To assess the sterilization efficiency with H₂O₂, bacterial spores are common microbial systems due to their remarkable robustness against a wide variety of decontamination strategies. Despite their widespread use, there is, however, only little information about the detailed time-resolved mechanism underlying the oxidative spore death by H₂O₂. In this work, we investigate chemical and morphological changes of individual Bacillus atrophaeus spores undergoing oxidative damage using optical sensing with trapping Raman microscopy in real-time. The time-resolved experiments reveal that spore death involves two distinct phases: (i) an initial phase dominated by the fast release of dipicolinic acid (DPA), a major spore biomarker, which indicates the rupture of the spore’s core; and (ii) the oxidation of the remaining spore material resulting in the subsequent fragmentation of the spores’ coat. Simultaneous observation of the spore morphology by optical microscopy corroborates these mechanisms. The dependence of the onset of DPA release and the time constant of spore fragmentation on H₂O₂ shows that the formation of reactive oxygen species from H₂O₂ is the rate-limiting factor of oxidative spore death.
KW  - DPA (dipicolinic acid)
KW  - sterilization
KW  - Bacillus atrophaeus spores
KW  - optical trapping
KW  - Raman spectroscopy
KW  - optical sensor setup
Y1  - 2023
U6  - http://dx.doi.org/10.3390/chemosensors11080445
SN  - 2227-9040
N1  - This article belongs to the Special Issue "Biosensors and Chemical Sensors for Food and Healthcare Monitoring—Celebrating the 10th Anniversary"
VL  - 8
IS  - 11
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Pfaff, Raphael
T1  - Braking distance prediction for vehicle consist in low-speed on-sight operation: a Monte Carlo approach
JF  - Railway Engineering Science
N2  - The first and last mile of a railway journey, in both freight and transit applications, constitutes a high effort and is either non-productive (e.g. in the case of depot operations) or highly inefficient (e.g. in industrial railways). These parts are typically managed on-sight, i.e. with no signalling and train protection systems ensuring the freedom of movement. This is possible due to the rather short braking distances of individual vehicles and shunting consists. The present article analyses the braking behaviour of such shunting units. For this purpose, a dedicated model is developed. It is calibrated on published results of brake tests and validated against a high-definition model for low-speed applications. Based on this model, multiple simulations are executed to obtain a Monte Carlo simulation of the resulting braking distances. Based on the distribution properties and established safety levels, the risk of exceeding certain braking distances is evaluated and maximum braking distances are derived. Together with certain parameters of the system, these can serve in the design and safety assessment of driver assistance systems and automation of these processes.
KW  - Freight rail
KW  - Shunting
KW  - Braking curves
KW  - Brake set-up
KW  - Driver assistance system
Y1  - 2023
U6  - http://dx.doi.org/10.1007/s40534-023-00303-7
SN  - 2662-4753 (eISSN)
SN  - 2662-4745 (Print)
VL  - 31
IS  - 2
SP  - 135
EP  - 144
PB  - SpringerOpen
ER  - 
TY  - JOUR
A1  - Herzwurm, Georg
A1  - Krams, Benedikt
A1  - Pietsch, Wolfram
A1  - Schockert, Sixten
T1  - Report from the 3rd international workshop on requirements prioritization for customer oriented software development (RePriCo’12)
JF  - ACM SIGSOFT Software Engineering Notes
N2  - Prioritization is an essential task within requirements engineering to cope with complexity and to establish focus properly. The 3rd Workshop on Requirements Prioritization for customer oriented Software Development (RePriCo’12) focused on requirements prioritization and adjacent themes in the context of customer oriented development of bespoke and standard software. Five submissions have been accepted for the proceedings and for presentation. The report summarizes and points out key findings.
KW  - Requirements relations
KW  - Discourse ethics
KW  - Tool support
KW  - Consensus
KW  - Requirements prioritization
Y1  - 2012
U6  - http://dx.doi.org/10.1145/2237796.2237817
SN  - 0163-5948
VL  - 37
IS  - 4
SP  - 32
EP  - 34
PB  - Association for Computing Machinery
CY  - New York
ER  - 
TY  - JOUR
A1  - Dellmann, Sophia Florence
A1  - Glorius, J.
A1  - Litvinov, Yu A.
A1  - Reifarth, R.
A1  - Al-Khasawneh, Kafa
A1  - Aliotta, M.
A1  - Bott, L.
A1  - Brückner, Benjamin
A1  - Bruno, C. G.
A1  - Chen, Ruijiu
A1  - Davinson, T.
A1  - Dickel, T.
A1  - Dillmann, Iris
A1  - Dmytriev, D.
A1  - Erbacher, P.
A1  - Freire-Fernández, D.
A1  - Forstner, Oliver
A1  - Geissel, H.
A1  - Göbel, K.
A1  - Griffin, Christopher J.
A1  - Grisenti, R.
A1  - Gumberidze, Alexandre
A1  - Haettner, Emma
A1  - Hagmann, Siegbert
A1  - Heil, M.
A1  - Heß, R.
A1  - Hillenbrand, P.-M.
A1  - Joseph, R.
A1  - Jurado, B.
A1  - Kozhuharov, Christophor
A1  - Kulikov, I.
A1  - Löher, Bastian
A1  - Langer, Christoph
A1  - Leckenby, Guy
A1  - Lederer-Woods, C.
A1  - Lestinsky, M.
A1  - Litvinov, S. A.
A1  - Lorenz, B. A.
A1  - Lorenz, E.
A1  - Marsh, J.
A1  - Menz, Esther Babette
A1  - Morgenroth, T.
A1  - Petridis, N.
A1  - Pibernat, Jerome
A1  - Popp, U.
A1  - Psaltis, Athanasios
A1  - Sanjari, Shahab
A1  - Scheidenberger, C.
A1  - Sguazzin, M.
A1  - Sidhu, Ragandeep Singh
A1  - Spillmann, Uwe
A1  - Steck, M.
A1  - Stöhlker, T.
A1  - Surzhykov, A.
A1  - Swartz, J. A.
A1  - Törnqvist, H.
A1  - Varga, L.
A1  - Vescovi, Diego
A1  - Weick, H.
A1  - Weigand, M.
A1  - Woods, P.
A1  - Xing, Y.
A1  - Yamaguchi, Taiyo
T1  - Proton capture on stored radioactive ¹¹⁸Te ions
JF  - EPJ Web of Conferences
N2  - Experimental determination of the cross sections of proton capture on radioactive nuclei is extremely difficult. Therefore, it is of substantial interest for the understanding of the production of the p-nuclei. For the first time, a direct measurement of proton-capture cross sections on stored, radioactive ions became possible in an energy range of interest for nuclear astrophysics. The experiment was performed at the Experimental Storage Ring (ESR) at GSI by making use of a sensitive method to measure (p,γ) and (p,n) reactions in inverse kinematics. These reaction channels are of high relevance for the nucleosyn-thesis processes in supernovae, which are among the most violent explosions in the universe and are not yet well understood. The cross section of the ¹¹⁸Te(p,γ) reaction has been measured at energies of 6 MeV/u and 7 MeV/u. The heavy ions interacted with a hydrogen gas jet target. The radiative recombination process of the fully stripped ¹¹⁸Te ions and electrons from the hydrogen target was used as a luminosity monitor. An overview of the experimental method and preliminary results from the ongoing analysis will be presented.
Y1  - 2023
U6  - http://dx.doi.org/10.1051/epjconf/202327911018
SN  - 2100-014X
N1  - Volume 279, 2023. Nuclear Physics in Astrophysics – X (NPA-X 2022).
VL  - 279
IS  - Article Number: 11018
SP  - 1
EP  - 5
PB  - EDP Sciences
ER  - 
TY  - JOUR
A1  - Falkenberg, Fabian
A1  - Kohn, Sophie
A1  - Bott, Michael
A1  - Bongaerts, Johannes
A1  - Siegert, Petra
T1  - Biochemical characterisation of a novel broad pH spectrum subtilisin from Fictibacillus arsenicus DSM 15822ᵀ
JF  - FEBS Open Bio
N2  - Subtilisins from microbial sources, especially from the Bacillaceae family, are of particular interest for biotechnological applications and serve the currently growing enzyme market as efficient and novel biocatalysts. Biotechnological applications include use in detergents, cosmetics, leather processing, wastewater treatment and pharmaceuticals. To identify a possible candidate for the enzyme market, here we cloned the gene of the subtilisin SPFA from Fictibacillus arsenicus DSM 15822ᵀ (obtained through a data mining-based search) and expressed it in Bacillus subtilis DB104. After production and purification, the protease showed a molecular mass of 27.57 kDa and a pI of 5.8. SPFA displayed hydrolytic activity at a temperature optimum of 80 °C and a very broad pH optimum between 8.5 and 11.5, with high activity up to pH 12.5. SPFA displayed no NaCl dependence but a high NaCl tolerance, with decreasing activity up to concentrations of 5 m NaCl. The stability enhanced with increasing NaCl concentration. Based on its substrate preference for 10 synthetic peptide 4-nitroanilide substrates with three or four amino acids and its phylogenetic classification, SPFA can be assigned to the subgroup of true subtilisins. Moreover, SPFA exhibited high tolerance to 5% (w/v) SDS and 5% H₂O₂ (v/v). The biochemical properties of SPFA, especially its tolerance of remarkably high pH, SDS and H₂O₂, suggest it has potential for biotechnological applications.
KW  - Bacillaceae
KW  - Biotechnological application
KW  - Broad pH spectrum
KW  - Subtilases
KW  - Subtilisin
Y1  - 2023
U6  - http://dx.doi.org/10.1002/2211-5463.13701
SN  - 2211-5463
N1  - Corresponding author: Petra Siegert
VL  - 13
IS  - 11
SP  - 2035
EP  - 2046
PB  - Wiley
CY  - Hoboken, NJ
ER  - 
TY  - JOUR
A1  - Cheenakula, Dheeraja
A1  - Paulsen, Svea
A1  - Ott, Fabian
A1  - Grömping, Markus
T1  - Operational window of a deammonifying sludge for mainstream application in a municipal wastewater treatment plant
JF  - Water and Environment Journal
N2  - The present work aimed to study the mainstream feasibility of the deammonifying sludge of side stream of municipal wastewater treatment plant (MWWTP) in Kaster, Germany. For this purpose, the deammonifying sludge available at the side stream was investigated for nitrogen (N) removal with respect to the operational factors temperature (15–30°C), pH value (6.0–8.0) and chemical oxygen demand (COD)/N ratio (≤1.5–6.0). The highest and lowest N-removal rates of 0.13 and 0.045 kg/(m³ d) are achieved at 30 and 15°C, respectively. Different conditions of pH and COD/N ratios in the SBRs of Partial nitritation/anammox (PN/A) significantly influenced both the metabolic processes and associated N-removal rates. The scientific insights gained from the current work signifies the possibility of mainstream PN/A at WWTPs. The current study forms a solid basis of operational window for the upcoming semi-technical trails to be conducted prior to the full-scale mainstream PN/A at WWTP Kaster and WWTPs globally.
KW  - Anammox
KW  - Mainstream
KW  - Nitrogen removal
KW  - Partial nitritation
KW  - Wastewater
Y1  - 2023
U6  - http://dx.doi.org/10.1111/wej.12898
SN  - 1747-6593
N1  - Corresponding author: Dheeraja Cheenakula
IS  - Early View
SP  - 1
EP  - 12
PB  - Wiley
CY  - Chichester
ER  - 
TY  - JOUR
A1  - Thoma, Andreas
A1  - Thomessen, Karolin
A1  - Gardi, Alessandro
A1  - Fisher, A.
A1  - Braun, Carsten
T1  - Prioritising paths: An improved cost function for local path planning for UAV in medical applications
JF  - The Aeronautical Journal
N2  - Even the shortest flight through unknown, cluttered environments requires reliable local path planning algorithms to avoid unforeseen obstacles. The algorithm must evaluate alternative flight paths and identify the best path if an obstacle blocks its way. Commonly, weighted sums are used here. This work shows that weighted Chebyshev distances and factorial achievement scalarising functions are suitable alternatives to weighted sums if combined with the 3DVFH* local path planning algorithm. Both methods considerably reduce the failure probability of simulated flights in various environments. The standard 3DVFH* uses a weighted sum and has a failure probability of 50% in the test environments. A factorial achievement scalarising function, which minimises the worst combination of two out of four objective functions, reaches a failure probability of 26%; A weighted Chebyshev distance, which optimises the worst objective, has a failure probability of 30%. These results show promise for further enhancements and to support broader applicability.
KW  - Path planning
KW  - Cost function
KW  - Multi-objective optimization
Y1  - 2023
U6  - http://dx.doi.org/10.1017/aer.2023.68
SN  - 0001-9240 (Print)
SN  - 2059-6464 (Online)
IS  - First View
SP  - 1
EP  - 18
PB  - Cambridge University Press
CY  - Cambridge
ER  - 
TY  - JOUR
A1  - Morais, Paulo V.
A1  - Suman, Pedro H.
A1  - Schöning, Michael Josef
A1  - Siqueira Junior, José R.
A1  - Orlandi, Marcelo O.
T1  - Layer-by-layer film based on Sn₃O₄ nanobelts as sensing units to detect heavy metals using a capacitive field-effect sensor platform
JF  - Chemosensors
N2  - Lead and nickel, as heavy metals, are still used in industrial processes, and are classified as “environmental health hazards” due to their toxicity and polluting potential. The detection of heavy metals can prevent environmental pollution at toxic levels that are critical to human health. In this sense, the electrolyte–insulator–semiconductor (EIS) field-effect sensor is an attractive sensing platform concerning the fabrication of reusable and robust sensors to detect such substances. This study is aimed to fabricate a sensing unit on an EIS device based on Sn₃O₄ nanobelts embedded in a polyelectrolyte matrix of polyvinylpyrrolidone (PVP) and polyacrylic acid (PAA) using the layer-by-layer (LbL) technique. The EIS-Sn₃O₄ sensor exhibited enhanced electrochemical performance for detecting Pb²⁺ and Ni²⁺ ions, revealing a higher affinity for Pb²⁺ ions, with sensitivities of ca. 25.8 mV/decade and 2.4 mV/decade, respectively. Such results indicate that Sn₃O₄ nanobelts can contemplate a feasible proof-of-concept capacitive field-effect sensor for heavy metal detection, envisaging other future studies focusing on environmental monitoring.
KW  - Sn₃O₄
KW  - nanobelts
KW  - field-effect sensor
KW  - LbL films
KW  - heavy metals
Y1  - 2023
U6  - http://dx.doi.org/10.3390/chemosensors11080436
SN  - 2227-9040
N1  - This article belongs to the Special Issue The Application of Electrochemical Sensors or Biosensors Based on Nanomaterials
VL  - 11
IS  - 8
PB  - MDPI
CY  - Basel
ER  -