TY  - JOUR
A1  - Arreola, Julio
A1  - Mätzkow, Malte
A1  - Durán, Marlena Palomar
A1  - Greeff, Anton
A1  - Keusgen, Michael
A1  - Schöning, Michael Josef
T1  - Optimization of the immobilization of bacterial spores on glass substrates with organosilanes
JF  - Physica status solidi (A) : Applications and materials science
N2  - Spores can be immobilized on biosensors to function as sensitive recognition elements. However, the immobilization can affect the sensitivity and reproducibility of the sensor signal. In this work, three different immobilization strategies with organosilanes were optimized and characterized to immobilize Bacillus atrophaeus spores on glass substrates. Five different silanization parameters were investigated: nature of the solvent, concentration of the silane, silanization time, curing process, and silanization temperature. The resulting silane layers were resistant to a buffer solution (e.g., Ringer solution) with a polysorbate (e.g., Tween®80) and sonication.
KW  - silanization
KW  - organosilanes
KW  - immobilization
KW  - endospores
KW  - biosensors
KW  - Bacillus atrophaeus
Y1  - 2016
U6  - http://dx.doi.org/10.1002/pssa.201532914
SN  - 1862-6319
VL  - 213
IS  - 6
SP  - 1463
EP  - 1470
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Arreola, Julio
A1  - Oberländer, Jan
A1  - Mätzkow, M.
A1  - Keusgen, Michael
A1  - Schöning, Michael Josef
T1  - Surface functionalization for spore-based biosensors with organosilanes
JF  - Electrochimica Acta
N2  - In the present work, surface functionalization of different sensor materials was studied. Organosilanes are well known to serve as coupling agent for biomolecules or cells on inorganic materials. 3-aminopropyltriethoxysilane (APTES) was used to attach microbiological spores time to an interdigitated sensor surface. The functionality and physical properties of APTES were studied on isolated sensor materials, namely silicon dioxide (SiO2) and platinum (Pt) as well as the combined material on sensor level. A predominant immobilization of spores could be demonstrated on SiO2 surfaces. Additionally, the impedance signal of APTES-functionalized biosensor chips has been investigated.
Y1  - 2017
U6  - http://dx.doi.org/10.1016/j.electacta.2017.04.157
SN  - 0013-4686
VL  - 241
SP  - 237
EP  - 243
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - CHAP
A1  - Oberländer, Jan
A1  - Arreola, Julio
A1  - Hansen, Christina
A1  - Greeff, Anton
A1  - Mayer, Marlena
A1  - Keusgen, Michael
A1  - Schöning, Michael Josef
T1  - Impedimetric Biosensor to Enable Fast Evaluation of Gaseous Sterilization Processes
T2  - MDPI Proceedings
Y1  - 2017
U6  - http://dx.doi.org/10.3390/proceedings1040435
N1  - Eurosensors 2017 Conference, Paris, France, 3–6 September 2017
VL  - 1
IS  - 4
ER  - 
TY  - JOUR
A1  - Arreola, Julio
A1  - Keusgen, Michael
A1  - Schöning, Michael Josef
T1  - Effect of O2 plasma on properties of electrolyte-insulator-semiconductor structures
JF  - physica status solidi a : applications and materials sciences
N2  - Prior to immobilization of biomolecules or cells onto biosensor surfaces, the surface must be physically or chemically activated for further functionalization. Organosilanes are a versatile option as they facilitate the immobilization through their terminal groups and also display self-assembly. Incorporating hydroxyl groups is one of the important methods for primary immobilization. This can be done, for example, with oxygen plasma treatment. However, this treatment can affect the performance of the biosensors and this effect is not quite well understood for surface functionalization. In this work, the effect of O2 plasma treatment on EIS sensors was investigated by means of electrochemical characterizations: capacitance–voltage (C–V) and constant capacitance (ConCap) measurements. After O2 plasma treatment, the potential of the EIS sensor dramatically shifts to a more negative value. This was successfully reset by using an annealing process.
KW  - surface functionalization
KW  - O2 plasma
KW  - hydroxylation
KW  - electrolyte-insulator semiconductor sensor (EIS)
KW  - annealing
Y1  - 2017
U6  - http://dx.doi.org/10.1002/pssa.201700025
SN  - 1862-6319
VL  - 214
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Arreola, Julio
A1  - Keusgen, Michael
A1  - Schöning, Michael Josef
T1  - Toward an immobilization method for spore-based biosensors in oxidative environment
JF  - Electrochimica Acta
Y1  - 2019
U6  - http://dx.doi.org/10.1016/j.electacta.2019.01.148
VL  - 302
SP  - 394
EP  - 401
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Arreola, Julio
A1  - Keusgen, Michael
A1  - Wagner, Torsten
A1  - Schöning, Michael Josef
T1  - Combined calorimetric gas- and spore-based biosensor array for online monitoring and sterility assurance of gaseous hydrogen peroxide in aseptic filling machines
JF  - Biosensors and Bioelectronics
Y1  - 2019
U6  - http://dx.doi.org/10.1016/j.bios.2019.111628
SN  - 0956-5663
VL  - 143
IS  - 111628
PB  - Elsevier
CY  - Amsterdam
ER  -