TY  - CHAP
A1  - Welden, Melanie
A1  - Severins, Robin
A1  - Poghossian, Arshak
A1  - Wege, Christina
A1  - Siegert, Petra
A1  - Keusgen, Michael
A1  - Schöning, Michael Josef
T1  - Studying the immobilization of acetoin reductase with Tobacco mosaic virus particles on capacitive field-effect sensors
T2  - 2022 IEEE International Symposium on Olfaction and Electronic Nose (ISOEN)
N2  - A capacitive electrolyte-insulator-semiconductor (EISCAP) biosensor modified with Tobacco mosaic virus (TMV) particles for the detection of acetoin is presented. The enzyme acetoin reductase (AR) was immobilized on the surface of the EISCAP using TMV particles as nanoscaffolds. The study focused on the optimization of the TMV-assisted AR immobilization on the Ta 2 O 5 -gate EISCAP surface. The TMV-assisted acetoin EISCAPs were electrochemically characterized by means of leakage-current, capacitance-voltage, and constant-capacitance measurements. The TMV-modified transducer surface was studied via scanning electron microscopy.
KW  - Tobacco mosaic virus
KW  - acetoin
KW  - capacitive field-effect biosensor
KW  - enzyme immobilization
Y1  - 2022
SN  - 978-1-6654-5860-3 (Online)
SN  - 978-1-6654-5861-0 (Print)
U6  - http://dx.doi.org/10.1109/ISOEN54820.2022.9789657
N1  - IEEE International Symposium on Olfaction and Electronic Nose (ISOEN), 29 May 2022 - 01 June 2022, Aveiro, Portugal.
PB  - IEEE
ER  - 
TY  - JOUR
A1  - Jablonski, Melanie
A1  - Münstermann, Felix
A1  - Nork, Jasmina
A1  - Molinnus, Denise
A1  - Muschallik, Lukas
A1  - Bongaerts, Johannes
A1  - Wagner, Torsten
A1  - Keusgen, Michael
A1  - Siegert, Petra
A1  - Schöning, Michael Josef
T1  - Capacitive field‐effect biosensor applied for the detection of acetoin in alcoholic beverages and fermentation broths
JF  - physica status solidi (a) applications and materials science
N2  - An acetoin biosensor based on a capacitive electrolyte–insulator–semiconductor (EIS) structure modified with the enzyme acetoin reductase, also known as butane-2,3-diol dehydrogenase (Bacillus clausii DSM 8716ᵀ), is applied for acetoin detection in beer, red wine, and fermentation broth samples for the first time. The EIS sensor consists of an Al/p-Si/SiO₂/Ta₂O₅ layer structure with immobilized acetoin reductase on top of the Ta₂O₅ transducer layer by means of crosslinking via glutaraldehyde. The unmodified and enzyme-modified sensors are electrochemically characterized by means of leakage current, capacitance–voltage, and constant capacitance methods, respectively.
KW  - acetoin
KW  - acetoin reductase
KW  - alcoholic beverages
KW  - biosensors
KW  - capacitive field-effect sensors
Y1  - 2021
U6  - http://dx.doi.org/10.1002/pssa.202000765
SN  - 1862-6319
VL  - 218
IS  - 13
PB  - Wiley-VCH
CY  - Weinheim
ER  -