TY  - CHAP
A1  - Brandes, Sinja
A1  - Epple, U.
A1  - Gligorevic, Snjezana
A1  - Schnell, Michael
A1  - Haindl, Bernhard
A1  - Sajatovic, Miodrag
T1  - Physical layer specification of the L-band Digital Aeronautical Communications System (L-DACS1)
T2  - Integrated Communications, Navigation and Surveillance Conference : ICNS '09 : 13 - 15 May 2009, Arlington, Va.
Y1  - 2009
SN  - 978-1-4244-4733-6 ; 978-1-4244-4734-3
SP  - 1
EP  - 12
ER  - 
TY  - CHAP
A1  - Epple, U.
A1  - Brandes, Sinja
A1  - Gligorevic, Snjezana
A1  - Schnell, Michael
T1  - Receiver optimization for L-DACS1
T2  - IEEE/AIAA 28th Digital Avionics Systems Conference : 23-29 Oct. 2009 : Orlando, Fla.
Y1  - 2009
SN  - 978-1-4244-4078-8
SP  - 4B1-1
EP  - 4B1-12
ER  - 
TY  - JOUR
A1  - Goßmann, Matthias
A1  - Frotscher, Ralf
A1  - Linder, Peter
A1  - Bayer, Robin
A1  - Epple, U.
A1  - Staat, Manfred
A1  - Temiz Artmann, Aysegül
A1  - Artmann, Gerhard
T1  - Mechano-pharmacological characterization of cardiomyocytes derived from human induced pluripotent stem cells
JF  - Cellular physiology and biochemistry
N2  - Background/Aims: Common systems for the quantification of cellular contraction rely on animal-based models, complex experimental setups or indirect approaches. The herein presented CellDrum technology for testing mechanical tension of cellular monolayers and thin tissue constructs has the potential to scale-up mechanical testing towards medium-throughput analyses. Using hiPS-Cardiac Myocytes (hiPS-CMs) it represents a new perspective of drug testing and brings us closer to personalized drug medication. Methods: In the present study, monolayers of self-beating hiPS-CMs were grown on ultra-thin circular silicone membranes and deflect under the weight of the culture medium. Rhythmic contractions of the hiPS-CMs induced variations of the membrane deflection. The recorded contraction-relaxation-cycles were analyzed with respect to their amplitudes, durations, time integrals and frequencies. Besides unstimulated force and tensile stress, we investigated the effects of agonists and antagonists acting on Ca²⁺ channels (S-Bay K8644/verapamil) and Na⁺ channels (veratridine/lidocaine). Results: The measured data and simulations for pharmacologically unstimulated contraction resembled findings in native human heart tissue, while the pharmacological dose-response curves were highly accurate and consistent with reference data. Conclusion: We conclude that the combination of the CellDrum with hiPS-CMs offers a fast, facile and precise system for pharmacological, toxicological studies and offers new preclinical basic research potential.
KW  - Inotropic compounds
KW  - Pharmacology
KW  - Ion channels
KW  - CellDrum
KW  - Heart tissue culture
KW  - Induced pluripotent stem cells
KW  - Cardiac myocytes
Y1  - 2016
U6  - http://dx.doi.org/10.1159/000443124
SN  - 1421-9778 (Online)
SN  - 1015-8987 (Print)
VL  - 38
IS  - 3
SP  - 1182
EP  - 1198
PB  - Karger
CY  - Basel
ER  -