TY  - GEN
A1  - Braun, Lena
A1  - Krafft, Simone
A1  - Tippkötter, Nils
T1  - Combined supercritical carbon dioxide extraction and chromatography of the algae fatty linoleic and linolenic acid
T2  - Chemie Ingenieur Technik
N2  - A method for the integrated extraction and separation of fatty acids from algae using supercritical CO2 is presented. Desmodesmus obliquus and Chlorella sorokiniana were used as algae. First, a method for chromatographic separation of fatty acids of different degrees of saturation was established and optimized. Then, an integrated method for supercritical extraction was developed for both algal species. It was also verified whether prior cell disruption was beneficial for extraction. In developing the method for chromatographic separation, statistical experimental design was used to determine the optimal parameter settings. The methanol content in the mobile phase proved to be the most important parameter for successful separation of the three unsaturated fatty acids oleic acid, linoleic acid, and linolenic acid. Supercritical extraction with dried algae showed that about four times more fatty acids can be extracted from C. sorokiniana relative to the dry mass used.
Y1  - 2022
U6  - https://doi.org/10.1002/cite.202255308
SN  - 0009-286X
SN  - 1522-2640 (eISSN)
N1  - ProcessNet and DECHEMA‐BioTechNet Jahrestagungen 2022 together with 13th ESBES Symposium 2022, 12. - 15. September 2022, Eurogress Aachen
VL  - 94
IS  - 9
SP  - 1304
PB  - Wiley-VCH
CY  - Weinheim
ER  -