TY  - CHAP
A1  - Savitskaya, Irina S.
A1  - Kistaubayeva, Aida S.
A1  - Akimbekov, Nuraly
A1  - Digel, Ilya
A1  - Shokatayeva, Dina
A1  - Zhubanova, Azhar Achmet
T1  - Prospective Use of Probiotics Immobilized on Sorbents with Nanostructured Surfaces
T2  - Carbon Nanomaterials in Biomedicine and the Environment
N2  - Activated carbons are known as excellent adsorbents. Their applications include the adsorptive removal of color, odor, taste, undesirable organic and inorganic pollutants from drinking and waste water; air purification in inhabited spaces; purification of many chemicals, pharmaceutical products and many others. This chapter elucidates the role of normal microflora in the maintenance of human health and presents materials on possible clinical displays of microecological infringements and ways of their correction. It presents new developments concerning new probiotics with immobilized Lactobacillus and Bacillus. The chapter considers the mechanisms of the intestine disbacteriosis correction by sorbed probiotics. It demonstrates the advantages and creation prospects of immobilized probiotics developed on the basis of carbonized rice husk. There are great prospects for the development of medical biotechnology due to use of carbon sorbents with a nanostructured surface. Microbial communities form a biocenosis of the biotope and together with the host organism create permanent or temporary ecosystems.
Y1  - 2020
SN  - 978-981-4800-27-3
U6  - http://dx.doi.org/10.1201/9780429428647-12
SP  - 229
EP  - 267
PB  - Jenny Stanford Publishing
CY  - Singapore
ER  - 
TY  - JOUR
A1  - Savitskaya, Irina
A1  - Zhantlessova, Sirina
A1  - Kistaubayeva, Aida
A1  - Ignatova, Ludmila
A1  - Shokatayeva, Dina
A1  - Sinyavsky, Yuriy
A1  - Kushugulova, Almagul
A1  - Digel, Ilya
T1  - Prebiotic cellulose–pullulan matrix as a “vehicle” for probiotic biofilm delivery to the host large intestine
JF  - Polymers
N2  - This study describes the development of a new combined polysaccharide-matrix-based technology for the immobilization of Lactobacillus rhamnosus GG (LGG) bacteria in biofilm form. The new composition allows for delivering the bacteria to the digestive tract in a manner that improves their robustness compared with planktonic cells and released biofilm cells. Granules consisting of a polysaccharide matrix with probiotic biofilms (PMPB) with high cell density (>9 log CFU/g) were obtained by immobilization in the optimized nutrient medium. Successful probiotic loading was confirmed by fluorescence microscopy and scanning electron microscopy. The developed prebiotic polysaccharide matrix significantly enhanced LGG viability under acidic (pH 2.0) and bile salt (0.3%) stress conditions. Enzymatic extract of feces, mimicking colon fluid in terms of cellulase activity, was used to evaluate the intestinal release of probiotics. PMPB granules showed the ability to gradually release a large number of viable LGG cells in the model colon fluid. In vivo, the oral administration of PMPB granules in rats resulted in the successful release of probiotics in the colon environment. The biofilm-forming incubation method of immobilization on a complex polysaccharide matrix tested in this study has shown high efficacy and promising potential for the development of innovative biotechnologies.
KW  - immobilization
KW  - prebiotic
KW  - bacterial cellulose
KW  - biofilms
KW  - Lactobacillus rhamnosus GG
Y1  - 2023
U6  - http://dx.doi.org/10.3390/polym16010030
N1  - This article belongs to the Section "Polymer Composites and Nanocomposites"
IS  - 16(1)
PB  - MDPI
CY  - Basel
ER  -