TY - JOUR A1 - Martin, S. A. A1 - Berg, G. P. A. A1 - Hacker, U. A1 - Hardt, Arno A1 - Köhler, M. A1 - Meißburger, J. A1 - Osterfeld, F. A1 - Prasuhn, D. A1 - Riepe, G. A1 - Rogge, M. A1 - Schult, O. W. B. A1 - Speth, J. A1 - Turek, P. A1 - Gaul, G. A1 - Hagedoorn, H. A1 - Heide, J. A. van der A1 - Hinterberger, F. A1 - Huber, M. A1 - Jahn, R. A1 - Mayer-Kuckuk, T. A1 - Poth, H. A1 - Paetz gen. Schieck, H. T1 - COSY - a cooler synchrotron and storage ring JF - IEEE transactions on nuclear science. Vol. 32, iss. 5 Y1 - 1985 SN - 1558-1578 (E-Journal); 0018-9499 (Print) SP - 2694 EP - 2696 ER - TY - BOOK A1 - Berg, G. A1 - Gaul, G. A1 - Hagedoorn, H. A1 - Hardt, Arno A1 - Heide, J. A. van der A1 - Hinterberger, F. A1 - Huber, Max A1 - Jahn, R. A1 - Martin, S. A1 - Mayer-Kuckuk, T. A1 - Osterfeld, F. A1 - Paetz gen. Schieck, H. A1 - Prasuhn, D. A1 - Riepe, G. A1 - Rogge, M. A1 - Rossen, P. von A1 - Schult, O. W. B. A1 - Speth, J. A1 - Turek, P. T1 - Studie zum Bau eines kombinierten Kühler-Synchrotron-Rings an der KFA Jülich: COSY-Studie. Spezielle Berichte der Kernforschungsanlage Jülich. Nr. 0242. Jül-Spez-242 Y1 - 1984 PB - Kernforschungsanlage CY - Jülich ER - TY - JOUR A1 - Ribitsch, D. A1 - Heumann, S. A1 - Karl, W. A1 - Gerlach, J. A1 - Leber, R. A1 - Birner-Gruenberger, R. A1 - Gruber, K. A1 - Eiteljoerg, I. A1 - Remler, P. A1 - Siegert, Petra A1 - Lange, J. A1 - Maurer, Karl-Heinz A1 - Berg, G. A1 - Guebitz, G. M. A1 - Schwab, H. T1 - Extracellular serine proteases from Stenotrophomonas maltophilia: Screening, isolation and heterologous expression in E. coli JF - Journal of biotechnology N2 - A large strain collection comprising antagonistic bacteria was screened for novel detergent proteases. Several strains displayed protease activity on agar plates containing skim milk but were inactive in liquid media. Encapsulation of cells in alginate beads induced protease production. Stenotrophomonas maltophilia emerged as best performer under washing conditions. For identification of wash-active proteases, four extracellular serine proteases called StmPr1, StmPr2, StmPr3 and StmPr4 were cloned. StmPr2 and StmPr4 were sufficiently overexpressed in E. coli. Expression of StmPr1 and StmPr3 resulted in unprocessed, insoluble protein. Truncation of most of the C-terminal domain which has been identified by enzyme modeling succeeded in expression of soluble, active StmPr1 but failed in case of StmPr3. From laundry application tests StmPr2 turned out to be a highly wash-active protease at 45 °C. Specific activity of StmPr2 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 17 ± 2 U/mg. In addition we determined the kinetic parameters and cleavage preferences of protease StmPr2. KW - Alginate beads KW - Stenotrophomonas maltophilia KW - Detergent protease Y1 - 2012 U6 - http://dx.doi.org/10.1016/j.jbiotec.2011.09.025 SN - 1873-4863 (E-Journal); 0168-1656 (Print) VL - 157 IS - 1 SP - 140 EP - 147 PB - Elsevier CY - Amsterdam ER - TY - CHAP A1 - Bechstedt, U. A1 - Berg, Georg P. A. A1 - Hacker, U. A1 - Hardt, Arno A1 - Hürlimann, W. A1 - Maier, R. A1 - Martin, S. A1 - Meissburger, J. A1 - Römer, J. G. M. A1 - Possen, P. von A1 - Sagefka, T. T1 - BIG KARL and COSY T2 - The art and science of magnet design. A Festschrift in honor of Klaus Halbach. Vol. 1 Y1 - 1995 SP - 19 EP - 28 PB - Lawrence Berkeley Lab., Univ. of California CY - Berkeley, CA. ER - TY - BOOK A1 - Hardt, Arno A1 - Müller, Gerhard A1 - Berg, G. P. A1 - Kelleter, H.-J. T1 - Aufbau eines Plastikszintillationszählers für den Magnetspektrographen "BIG KARL" am Jülicher Zyklotron "JULIC". Müller, Gerhard; Berg , G. P.; Hardt, A.; Kelleter, H.-J.; Martin, S.-A.; Meissburger, J. Y1 - 1980 N1 - Spezielle Berichte der Kernforschungsanlage Jülich ; Nr. 0084 PB - Kernforschungsanlage Jülich CY - Jülich ER - TY - JOUR A1 - Martin, Siegfried A. A1 - Hardt, Arno A1 - Meissburger, Jürgen A1 - Berg, Georg P. A. A1 - Hacker, Ulrich A1 - Hürlimann, Werner A1 - Römer, Josef G. M. A1 - Sagefka, Thomas A1 - Retz, Adolf A1 - Schult, Otto W. B. T1 - The QQDDQ magnet spectrometer “BIG KARL” JF - Nuclear instruments and methods in physics research. Vol. 214, iss. 2-3 Y1 - 1983 SN - 0029-554x (E-Journal); 1872-9606 (E-Journal); 0167-5087 (Print) SP - 281 EP - 303 ER - TY - JOUR A1 - Berg, G. P. A. A1 - Demarteau, M. A1 - Hardt, Arno A1 - Hürlimann, W. A1 - Martin, S. A. A1 - Meissburger, J. A1 - Oelert, W. A1 - Seyfart, H. A1 - Styczen, B. A1 - Köhler, M. A1 - Oelrich, I. A1 - Scheerer, J. T1 - High resolution spectroscopy of 102Ru(d, p)103Ru and 104Ru(p, d)103Ru reactions JF - Nuclear Physics A. Vol. 379, iss. 1 Y1 - 1982 SN - 1873-1554 (E-Journal); 0375-9474 (Print) SP - 93 EP - 109 ER -