TY - JOUR A1 - Seifarth, Volker A1 - Schehl, D. A1 - Linder, Peter A1 - Gossmann, Matthias A1 - Digel, Ilya A1 - Artmann, Gerhard A1 - Porst, Dariusz A1 - Preiß, C. A1 - Kayser, Peter A1 - Pack, O. A1 - Temiz Artmann, Aysegül T1 - Ureplace: development of a bioreactor for in vitro culturing of cell seeded tubular vessels on collagen scaffolds N2 - The demand of replacements for inoperable organs exceeds the amount of available organ transplants. Therefore, tissue engineering developed as a multidisciplinary field of research for autologous in-vitro organs. Such three dimensional tissue constructs request the application of a bioreactor. The UREPLACE bioreactor is used to grow cells on tubular collagen scaffolds OPTIMAIX Sponge 1 with a maximal length of 7 cm, in order to culture in vitro an adequate ureter replacement. With a rotating unit, (urothelial) cells can be placed homogeneously on the inner scaffold surface. Furthermore, a stimulation is combined with this bioreactor resulting in an orientation of muscle cells. These culturing methods request a precise control of several parameters and actuators. A combination of a LabBox and the suitable software LabVision is used to set and conduct parameters like rotation angles, velocities, pressures and other important cell culture values. The bioreactor was tested waterproof successfully. Furthermore, the temperature controlling was adjusted to 37 °C and the CO2 - concentration regulated to 5 %. Additionally, the pH step responses of several substances showed a perfect functioning of the designed flow chamber. All used software was tested and remained stable for several days. KW - Tissue Engineering KW - Bioreaktor KW - Organkultur KW - Harnleiter Y1 - 2011 ER - TY - JOUR A1 - Kurulgan Demirci, Eylem A1 - Demirci, T. A1 - Trzewik, Jürgen A1 - Linder, Peter A1 - Karakulah, G. A1 - Artmann, Gerhard A1 - Sakizli, M. A1 - Temiz Artmann, Aysegül T1 - Genome-Wide Gene Expression Analysis of NIH 3T3 Cell Line Under Mechanical Stimulation JF - Cellular and molecular bioengineering. 4 (2011), H. 1 Y1 - 2011 SN - 1865-5025 SP - 46 EP - 55 PB - Springer CY - Berlin ER - TY - CHAP A1 - Duong, Minh Tuan A1 - Seifarth, Volker A1 - Temiz Artmann, Aysegül A1 - Artmann, Gerhard A1 - Staat, Manfred ED - Artmann, Gerhard ED - Temiz Artmann, Aysegül ED - Zhubanova, Azhar A. ED - Digel, Ilya T1 - Growth Modelling Promoting Mechanical Stimulation of Smooth Muscle Cells of Porcine Tubular Organs in a Fibrin-PVDF Scaffold T2 - Biological, Physical and Technical Basics of Cell Engineering N2 - Reconstructive surgery and tissue replacements like ureters or bladders reconstruction have been recently studied, taking into account growth and remodelling of cells since living cells are capable of growing, adapting, remodelling or degrading and restoring in order to deform and respond to stimuli. Hence, shapes of ureters or bladders and their microstructure change during growth and these changes strongly depend on external stimuli such as training. We present the mechanical stimulation of smooth muscle cells in a tubular fibrin-PVDFA scaffold and the modelling of the growth of tissue by stimuli. To this end, mechanotransduction was performed with a kyphoplasty balloon catheter that was guided through the lumen of the tubular structure. The bursting pressure was examined to compare the stability of the incubated tissue constructs. The results showed the significant changes on tissues with training by increasing the burst pressure as a characteristic mechanical property and the smooth muscle cells were more oriented with uniformly higher density. Besides, the computational growth models also exhibited the accurate tendencies of growth of the cells under different external stimuli. Such models may lead to design standards for the better layered tissue structure in reconstructing of tubular organs characterized as composite materials such as intestines, ureters and arteries. KW - Mechanical simulation KW - Growth modelling KW - Ureter KW - Bladder KW - Reconstruction Y1 - 2018 SN - 978-981-10-7904-7 U6 - https://doi.org/10.1007/978-981-10-7904-7_9 SP - 209 EP - 232 PB - Springer CY - Singapore ER - TY - JOUR A1 - Linder, Peter A1 - Digel, Ilya A1 - Temiz Artmann, Aysegül A1 - Kayser, Peter A1 - Porst, Dariusz A1 - Artmann, Gerhard T1 - High-throughput testing of mechanical forces generated in thin cell and tissue layers JF - Tissue Engineering. 13 (2007), H. 7 Y1 - 2007 SN - 1076-3279 N1 - Meeting abstract 433 SP - 1778 EP - 1778 ER - TY - JOUR A1 - Uysal, Karya A1 - Firat, Ipek Serat A1 - Creutz, Till A1 - Aydin, Inci Cansu A1 - Artmann, Gerhard A1 - Teusch, Nicole A1 - Temiz Artmann, Aysegül T1 - A novel in vitro wound healing assay using free-standing, ultra-thin PDMS membranes JF - membranes N2 - Advances in polymer science have significantly increased polymer applications in life sciences. We report the use of free-standing, ultra-thin polydimethylsiloxane (PDMS) membranes, called CellDrum, as cell culture substrates for an in vitro wound model. Dermal fibroblast monolayers from 28- and 88-year-old donors were cultured on CellDrums. By using stainless steel balls, circular cell-free areas were created in the cell layer (wounding). Sinusoidal strain of 1 Hz, 5% strain, was applied to membranes for 30 min in 4 sessions. The gap circumference and closure rate of un-stretched samples (controls) and stretched samples were monitored over 4 days to investigate the effects of donor age and mechanical strain on wound closure. A significant decrease in gap circumference and an increase in gap closure rate were observed in trained samples from younger donors and control samples from older donors. In contrast, a significant decrease in gap closure rate and an increase in wound circumference were observed in the trained samples from older donors. Through these results, we propose the model of a cell monolayer on stretchable CellDrums as a practical tool for wound healing research. The combination of biomechanical cell loading in conjunction with analyses such as gene/protein expression seems promising beyond the scope published here. Y1 - 2022 U6 - https://doi.org/10.3390/membranes13010022 N1 - This article belongs to the Special Issue "Latest Scientific Discoveries in Polymer Membranes" VL - 2023 IS - 13(1) PB - MDPI CY - Basel ER - TY - CHAP A1 - Bayer, Robin A1 - Hescheler, Jürgen A1 - Artmann, Gerhard A1 - Temiz Artmann, Aysegül ED - Staat, Manfred ED - Erni, Daniel T1 - Treating arterial hypertension in a cell culture well T2 - 3rd YRA MedTech Symposium 2019 : May 24 / 2019 / FH AachenW N2 - Hypertension describes the pathological increase of blood pressure, which is most commonly associated with the increase of vascular wall stiffness [1]. Referring to the “Deutsche Bluthochdruck Liga” this pathology shows a growing trend in our aging society. In order to find novel pharmacological and probably personalized treatments, we want to present a functional approach to study biomechanical properties of a human aortic vascular model. In this method review we will give an overview of recent studies which were carried out with the CellDrum technology [2] and underline the added value to already existing standard procedures known from the field of physiology. Herein described CellDrum technology is a system to measure functional mechanical properties of cell monolayers and thin tissue constructs in-vitro. Additionally, the CellDrum enables to elucidate the mechanical response of cells to pharmacological drugs, toxins and vasoactive agents. Due to its highly flexible polymer support, cells can also be mechanically stimulated by steady and cyclic biaxial stretching. Y1 - 2019 SN - 978-3-940402-22-6 U6 - https://doi.org/10.17185/duepublico/48750 SP - 5 EP - 6 PB - Universität Duisburg-Essen CY - Duisburg ER - TY - JOUR A1 - Goßmann, Matthias A1 - Frotscher, Ralf A1 - Linder, Peter A1 - Bayer, Robin A1 - Epple, U. A1 - Staat, Manfred A1 - Temiz Artmann, Aysegül A1 - Artmann, Gerhard T1 - Mechano-pharmacological characterization of cardiomyocytes derived from human induced pluripotent stem cells JF - Cellular physiology and biochemistry N2 - Background/Aims: Common systems for the quantification of cellular contraction rely on animal-based models, complex experimental setups or indirect approaches. The herein presented CellDrum technology for testing mechanical tension of cellular monolayers and thin tissue constructs has the potential to scale-up mechanical testing towards medium-throughput analyses. Using hiPS-Cardiac Myocytes (hiPS-CMs) it represents a new perspective of drug testing and brings us closer to personalized drug medication. Methods: In the present study, monolayers of self-beating hiPS-CMs were grown on ultra-thin circular silicone membranes and deflect under the weight of the culture medium. Rhythmic contractions of the hiPS-CMs induced variations of the membrane deflection. The recorded contraction-relaxation-cycles were analyzed with respect to their amplitudes, durations, time integrals and frequencies. Besides unstimulated force and tensile stress, we investigated the effects of agonists and antagonists acting on Ca²⁺ channels (S-Bay K8644/verapamil) and Na⁺ channels (veratridine/lidocaine). Results: The measured data and simulations for pharmacologically unstimulated contraction resembled findings in native human heart tissue, while the pharmacological dose-response curves were highly accurate and consistent with reference data. Conclusion: We conclude that the combination of the CellDrum with hiPS-CMs offers a fast, facile and precise system for pharmacological, toxicological studies and offers new preclinical basic research potential. KW - Inotropic compounds KW - Pharmacology KW - Ion channels KW - CellDrum KW - Heart tissue culture KW - Induced pluripotent stem cells KW - Cardiac myocytes Y1 - 2016 U6 - https://doi.org/10.1159/000443124 SN - 1421-9778 (Online) SN - 1015-8987 (Print) VL - 38 IS - 3 SP - 1182 EP - 1198 PB - Karger CY - Basel ER - TY - JOUR A1 - Artmann, Gerhard A1 - Trzewik, Jürgen A1 - Ates, M. T1 - A novel method to quantify mechanical tension in cell monolayers. Trzewik, Jürgen; Ates, M., Artmann, Gerhard Michael JF - Biomedizinische Technik. 47 (2002), H. Suppl. 1. Pt. 1 Y1 - 2002 SN - 0013-5585 N1 - Druckausgabe unter 63 Z 47 vorhanden SP - 379 EP - 381 ER - TY - JOUR A1 - Maggakis-Kelemen, Christina A1 - Biselli, Manfred A1 - Artmann, Gerhard T1 - Determination of the elastic shear modulus of cultured human red blood cells JF - Biomedizinische Technik. 47 (2002), H. Suppl. 1 Pt. 1 Y1 - 2002 SN - 0013-5585 N1 - Druckausgabe unter 63 Z 471 vorhanden SP - 106 EP - 109 ER - TY - JOUR A1 - Artmann, Gerhard A1 - Zhou-Stache, J. A1 - Buettner, R. A1 - Mittermayer, C. [u.a.] T1 - Inhibition of TNF-alpha induced cell death in HUVEC and Jurkat cells by protocatechuic acid. Zhou-Stache, J.; Buettner, R.; Artmann, Gerhard Michael; Mittermayer, C.; Bosserhoff, A. K. JF - Medical and Biological Engineering and Computing. 40 (2002), H. 6 Y1 - 2002 SN - 0140-0118 SP - 698 EP - 703 ER - TY - JOUR A1 - Artmann, Gerhard A1 - Hueck, Isgard S. A1 - Rossiter, Katharine A1 - Schmid-Schönbein, Geert W. T1 - Fluid Shear Attenuates Endothelial Pseudopodia Formation into the Capillary Lumen / Hueck, Isgard S. ; Rossiter, Katharine ; Artman, Gerhard M. ; Schmid-Schönbein, Geert W. JF - Microcirculation. 15 (2008), H. 6 Y1 - 2008 SN - 1549-8719 SP - 531 EP - 542 ER - TY - BOOK A1 - Artmann, Gerhard T1 - Bioengineering in Cell and Tissue Research / Artmann, Gerhard M. ; Chien, Shu (Eds.) Y1 - 2008 SN - 978-3-540-75408-4 PB - Springer CY - Berlin ER - TY - BOOK A1 - Artmann, Gerhard T1 - Stem cell engineering : principles and applications / Gerhard M. Artmann ... eds. Y1 - 2011 SN - 978-3-642-11864-7 PB - Springer CY - Berlin [u.a.] ER - TY - JOUR A1 - Eijck, Lambert van A1 - Demmel, Franz A1 - Artmann, Gerhard A1 - Stadtler, Andreas Maximilian T1 - Macromolecular dynamics in red blood cells investigated using neutron spectroscopy JF - Journal of the Royal Society Interface Y1 - 2011 SN - 1742-5689 VL - 8 IS - 57 SP - 590 EP - 600 PB - The Royal Society CY - London ER - TY - JOUR A1 - Becker, C. A1 - Wallang, C. A1 - Artmann, Gerhard A1 - Jakse, G. T1 - Mechanotransduction-bioreactor for tissue engineering of a ureter prosthesis JF - International Journal of Artificial Organs, The Y1 - 2008 SN - 0391-3988 N1 - Abstracts from the XXXV Congress of the European Society for Artificial Organs: 'Towards Future Biomedical Technologies' - ORAL PRESENTATIONS VL - 31 IS - 7 SP - 583 EP - 583 ER - TY - JOUR A1 - Maggakis-Kelemen, C. A1 - Bork, M. A1 - Kayser, Peter A1 - Biselli, Manfred A1 - Artmann, Gerhard T1 - Biological and mechanical quality of red blood cells cultured from human umbilical cord blood stem cells JF - Medical and biological engineering and computing. 41 (2003), H. 3 Y1 - 2003 SN - 0140-0118 SP - 350 EP - 356 ER - TY - JOUR A1 - Kaul, D. K. A1 - Koshkaryev, A. A1 - Artmann, Gerhard A1 - Barshtein, G. A1 - Yedgar, S. T1 - Additive effect of red blood cell rigidity and adherence to endothelial cells in inducing vascular resistance JF - American Journal of Physiology : Heart and Circulation Physiology . 295 (2008), H. 4 Y1 - 2008 SN - 1522-1539 VL - 295 IS - 4 SP - H1788 EP - H1793 ER - TY - JOUR A1 - Stadler, Alexander Maximilian A1 - Garvey, Christopher J. A1 - Embs, Jan Peter A1 - Koza, Michael Marek A1 - Unruh, Tobias A1 - Artmann, Gerhard A1 - Zaccai, Guiseppe T1 - Picosecond dynamics in haemoglobin from different species: A quasielastic neutron scattering study JF - Biochimica et biophysica acta (BBA): General Subjects Y1 - 2014 U6 - https://doi.org/10.1016/j.bbagen.2014.06.007 SN - 1872-8006 (E-Journal); 0304-4165 (Print) VL - 1840 IS - 10 SP - 2989 EP - 2999 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Zhubanova, Azhar A. A1 - Digel, Ilya A1 - Nojima, H. A1 - Artmann, Gerhard T1 - The use of bactericidal effects of cluster ions generated by plasma in medical biotechnology N2 - The most of conventional methods of air purification use the power of a fan to draw in air and pass it through a filter. The problem of bacterial contamination of inner parts of such a type of air conditioners in some cases draws attention towards alternative air-cleaning systems. Some manufacturers offer to use the ozone's bactericidal and deodorizing effects, but the wide spreading of such systems is restricted by the fact that toxic effects of ozone in respect of human beings are well known. In 2000 Sharp Inc. introduced "Plasma Cluster Ions (PCI)" air purification technology, which uses plasma discharge to generate cluster ions (I 0-14 ). This technology has been developed for those customers that are conscious about health and hygiene. In our experiments, we focused on some principal aspects of plasma-generated ions application - time-dependency and irreversibility of bactericidal action, spatial and kinetic characteristics of emitted cluster particles, their chemical targets in the microbial cells. KW - Clusterion KW - Raumluft KW - Luftreiniger KW - Plasmacluster ion technology KW - Air purification Y1 - 2007 ER - TY - CHAP A1 - Stadler, Andreas M. A1 - Embs, Jan P. A1 - Zerlin, Kay A1 - Digel, Ilya A1 - Artmann, Gerhard A1 - Zaccai, Joe A1 - Büldt, Georg T1 - Temperature transitions of hemoglobin and cytosolic water diffusion in human red blood cells : [poster] N2 - Background Hemoglobin interactions in red blood cells Hemoglobin dynamics in human red blood cells Diffusion of H2O in red blood cells KW - Erythrozyt KW - Hämoglobin KW - cytosolic water diffusion KW - hemoglobin dynamics Y1 - 2007 ER -