TY  - JOUR
A1  - Ribitsch, D.
A1  - Heumann, S.
A1  - Karl, W.
A1  - Gerlach, J.
A1  - Leber, R.
A1  - Birner-Gruenberger, R.
A1  - Gruber, K.
A1  - Eiteljoerg, I.
A1  - Remler, P.
A1  - Siegert, Petra
A1  - Lange, J.
A1  - Maurer, Karl-Heinz
A1  - Berg, G.
A1  - Guebitz, G. M.
A1  - Schwab, H.
T1  - Extracellular serine proteases from Stenotrophomonas maltophilia: Screening, isolation and heterologous expression in E. coli
JF  - Journal of biotechnology
N2  - A large strain collection comprising antagonistic bacteria was screened for novel detergent proteases. Several strains displayed protease activity on agar plates containing skim milk but were inactive in liquid media. Encapsulation of cells in alginate beads induced protease production. Stenotrophomonas maltophilia emerged as best performer under washing conditions. For identification of wash-active proteases, four extracellular serine proteases called StmPr1, StmPr2, StmPr3 and StmPr4 were cloned. StmPr2 and StmPr4 were sufficiently overexpressed in E. coli. Expression of StmPr1 and StmPr3 resulted in unprocessed, insoluble protein. Truncation of most of the C-terminal domain which has been identified by enzyme modeling succeeded in expression of soluble, active StmPr1 but failed in case of StmPr3.

From laundry application tests StmPr2 turned out to be a highly wash-active protease at 45 °C. Specific activity of StmPr2 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 17 ± 2 U/mg. In addition we determined the kinetic parameters and cleavage preferences of protease StmPr2.
KW  - Alginate beads
KW  - Stenotrophomonas maltophilia
KW  - Detergent protease
Y1  - 2012
U6  - https://doi.org/10.1016/j.jbiotec.2011.09.025
SN  - 1873-4863 (E-Journal); 0168-1656 (Print)
VL  - 157
IS  - 1
SP  - 140
EP  - 147
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Ribitsch, D.
A1  - Heumann, S.
A1  - Trotscha, E.
A1  - Herrero Acero, E.
A1  - Greimel, K.
A1  - Leber, R.
A1  - Birger-Gruenberger, R.
A1  - Deller, S.
A1  - Eiteljoerg, I.
A1  - Remler, P.
A1  - Weber, Th.
A1  - Siegert, Petra
A1  - Maurer, Karl-Heinz
A1  - Donelli, I.
A1  - Freddi, G.
A1  - Schwab, H.
A1  - Guebitz, G. M.
T1  - Hydrolysis of polyethyleneterephthalate by p-nitrobenzylesterase from Bacillus subtilis
JF  - Biotechnology progress
Y1  - 2011
SN  - 1520-6033 (E-Journal); 8756-7938 (Print)
VL  - Vol. 27
IS  - Iss. 4
SP  - 951
EP  - 960
PB  - Wiley
CY  - Hoboken
ER  - 
TY  - BOOK
A1  - Faßbender, Heinz
A1  - Bühler, Gerhard
A1  - Markmann, G.
A1  - Wunder, M.
T1  - Protokolle zur Replikation verteilter Daten
Y1  - 2001
N1  - (FKIE-Bericht ; 27)
PB  - FGAN-FKIE
CY  - Wachtberg
ER  - 
TY  - CHAP
A1  - Heinz, G.
A1  - Oertel, Mario
A1  - Bung, Daniel Bernhard
ED  - Janssen, Robert
T1  - Investigations on a cooling water system for a gas tubine facility concerning air vortexes and sediment transport
T2  - Hydraulic structures: useful water harvesting systems or relics? : Third International Junior Researcher and Engineer Workshop on Hydraulic Structures (IJREWHS'10) : Edinburgh, Scotland, U.K., 2-3 May 2010
Y1  - 2010
SN  - 9781742720159
SP  - 109
EP  - 116
PB  - School of Civil Engineering, The University of Queensland
CY  - Brisbane
ER  - 
TY  - CHAP
A1  - Knott, Thomas C.
A1  - Sofronia, Raluca E.
A1  - Gerressen, Marcus
A1  - Law, Yuen
A1  - Davidescu, Arjana
A1  - Savii, George G.
A1  - Gatzweiler, Karl-Heinz
A1  - Staat, Manfred
A1  - Kuhlen, Torsten W.
T1  - Preliminary bone sawing model for a virtual reality-based training simulator of bilateral sagittal split osteotomy
T2  - Biomedical simulation : 6th International Symposium, ISBMS 2014, Strasbourg, France, October 16-17, 2014 : proceedings (Lecture notes in computer science : vol. 8789)
N2  - Successful bone sawing requires a high level of skill and experience, which could be gained by the use of Virtual Reality-based simulators. A key aspect of these medical simulators is realistic force feedback. The aim of this paper is to model the bone sawing process in order to develop a valid training simulator for the bilateral sagittal split osteotomy, the most often applied corrective surgery in case of a malposition of the mandible. Bone samples from a human cadaveric mandible were tested using a designed experimental system. Image processing and statistical analysis were used for the selection of four models for the bone sawing process. The results revealed a polynomial dependency between the material removal rate and the applied force. Differences between the three segments of the osteotomy line and between the cortical and cancellous bone were highlighted.
KW  - Bone sawing
KW  - virtual reality
KW  - training simulator
Y1  - 2014
SN  - 978-3-319-12057-7 (Online)
SN  - 978-3-319-12056-0 (Print)
U6  - https://doi.org/10.1007/978-3-319-12057-7_1
SP  - 1
EP  - 10
PB  - Springer
CY  - Cham
ER  -