TY - CHAP A1 - Artmann, Gerhard A1 - Stadler, Andreas M. A1 - Embs, Jan P. A1 - Zaccai, Giuseppe A1 - Büldt, Georg A1 - Digel, Ilya A1 - Temiz Artmann, Aysegül T1 - The crucial role of water in a phase transition of hemoglobin at body temperature : [abstract] N2 - The observation of a temperature transition of hemoglobin occurring at a critical temperature close to body temperature KW - Hämoglobin KW - Erythrozyt KW - Körpertemperatur Y1 - 2010 ER - TY - JOUR A1 - Akimbekov, Nuraly S. A1 - Digel, Ilya A1 - Abdieva, Gulzhamal A1 - Ualieva, Perizat A1 - Tastambek, Kuanysh T1 - Lignite biosolubilization and bioconversion by Bacillus sp.: the collation of analytical data JF - Biofuels N2 - The vast metabolic potential of microbes in brown coal (lignite) processing and utilization can greatly contribute to innovative approaches to sustainable production of high-value products from coal. In this study, the multi-faceted and complex coal biosolubilization process by Bacillus sp. RKB 7 isolate from the Kazakhstan coal-mining soil is reported, and the derived products are characterized. Lignite solubilization tests performed for surface and suspension cultures testify to the formation of numerous soluble lignite-derived substances. Almost 24% of crude lignite (5% w/v) was solubilized within 14 days under slightly alkaline conditions (pH 8.2). FTIR analysis revealed various functional groups in the obtained biosolubilization products. Analyses of the lignite-derived humic products by UV-Vis and fluorescence spectrometry as well as elemental analysis yielded compatible results indicating the emerging products had a lower molecular weight and degree of aromaticity. Furthermore, XRD and SEM analyses were used to evaluate the biosolubilization processes from mineralogical and microscopic points of view. The findings not only contribute to a deeper understanding of microbe–mineral interactions in coal environments, but also contribute to knowledge of coal biosolubilization and bioconversion with regard to sustainable production of humic substances. The detailed and comprehensive analyses demonstrate the huge biotechnological potential of Bacillus sp. for agricultural productivity and environmental health. KW - humic acid KW - Bacillus sp KW - lignite KW - Biosolubilization Y1 - 2021 SN - 1759-7277 VL - 12 IS - 3 SP - 247 EP - 258 PB - Taylor & Francis CY - London ER - TY - JOUR A1 - Digel, Ilya A1 - Akimbekov, Nuraly S. A1 - Rogachev, Evgeniy A1 - Pogorelova, Natalia T1 - Bacterial cellulose produced by Medusomyces gisevii on glucose and sucrose: biosynthesis and structural properties JF - Cellulose N2 - In this work, the effects of carbon sources and culture media on the production and structural properties of bacterial cellulose (BC) synthesized by Medusomyces gisevii have been studied. The culture medium was composed of different initial concentrations of glucose or sucrose dissolved in 0.4% extract of plain green tea. Parameters of the culture media (titratable acidity, substrate conversion degree etc.) were monitored daily for 20 days of cultivation. The BC pellicles produced on different carbon sources were characterized in terms of biomass yield, crystallinity and morphology by field emission scanning electron microscopy (FE-SEM), atomic force microscopy and X-ray diffraction. Our results showed that Medusomyces gisevii had higher BC yields in media with sugar concentrations close to 10 g L−1 after a 18–20 days incubation period. Glucose in general lead to a higher BC yield (173 g L−1) compared to sucrose (163.5 g L−1). The BC crystallinity degree and surface roughness were higher in the samples synthetized from sucrose. Obtained FE-SEM micrographs show that the BC pellicles synthesized in the sucrose media contained densely packed tangles of cellulose fibrils whereas the BC produced in the glucose media displayed rather linear geometry of the BC fibrils without noticeable aggregates. KW - Bacterial cellulose KW - Medusomyces gisevi KW - Carbon sources KW - Culture media KW - Cellulose nanostructure Y1 - 2023 U6 - https://doi.org/10.1007/s10570-023-05592-z SN - 1572-882X (Online) SN - 0969-0239 (Print) N1 - Corresponding author: Ilya Digel PB - Springer Science + Business Media CY - Dordrecht ER - TY - JOUR A1 - Turaliyeva, M. A1 - Yeshibaev, A. A1 - Digel, Ilya A1 - Elibayeva, G. A1 - Sydykova, A. A1 - Uspabayeva, A. A1 - Dosybayeva, G. A1 - Zhylysbayeva, A. A1 - Lakhanova, K. T1 - Molecular-genetic identification of emerged novel invasive pathogens of Asiatic Elm Ulmus pumila L JF - Life science journal N2 - The dwarf elm Ulmus pumila L. (Ulmaceae) is one of indigenous species of flora in Kazakhstan and forms a basis of dendroflora in virtually all settlements of the region. In the past decade, multiple outbreaks of previously unknown diseases of the small-leaved elm have been registered. In our study, by the molecular-genetic analysis it was found that the pathogens responsible for the outbreaks are microfungi belonging to the genus Fusarium – F. solani and F. oxysporum. The nucleotide sequences (ITS regions) isolated from the diseased trees showed very high similarity with the GenBank control numbers EU625403.1 and FJ478128.1 (100.0 and 99.0 % respectively). Oncoming research will focus on the search of natural microbial antagonists of the discovered phytopathogens. Y1 - 2014 U6 - https://doi.org/10.7537/marslsj1105s14.33 SN - 1097-8135 VL - 11 IS - Spec. iss. 5s SP - 171 EP - 175 PB - Marsland Press CY - New York ER - TY - JOUR A1 - Zhubanova, Azhar A. A1 - Digel, Ilya A1 - Nojima, H. A1 - Artmann, Gerhard T1 - The use of bactericidal effects of cluster ions generated by plasma in medical biotechnology N2 - The most of conventional methods of air purification use the power of a fan to draw in air and pass it through a filter. The problem of bacterial contamination of inner parts of such a type of air conditioners in some cases draws attention towards alternative air-cleaning systems. Some manufacturers offer to use the ozone's bactericidal and deodorizing effects, but the wide spreading of such systems is restricted by the fact that toxic effects of ozone in respect of human beings are well known. In 2000 Sharp Inc. introduced "Plasma Cluster Ions (PCI)" air purification technology, which uses plasma discharge to generate cluster ions (I 0-14 ). This technology has been developed for those customers that are conscious about health and hygiene. In our experiments, we focused on some principal aspects of plasma-generated ions application - time-dependency and irreversibility of bactericidal action, spatial and kinetic characteristics of emitted cluster particles, their chemical targets in the microbial cells. KW - Clusterion KW - Raumluft KW - Luftreiniger KW - Plasmacluster ion technology KW - Air purification Y1 - 2007 ER - TY - JOUR A1 - Bogoyavlenskiy, A. P. A1 - Digel, Ilya A1 - Berezin, V. E. T1 - Assessment of dot-blot ELISA sensitivity on membrane sorbent using various peroxidase substrates N2 - The sensitivity of the peroxidase reaction in dot-blot ELISA significantly depends on the substrate. The highest sensitivity is observed using benzidine and diamine- phenol combinations as the substrates due to the reaction of the coupled oxidation (NADI) KW - Enzyme-linked immunosorbent assay KW - Peroxidase KW - ELISA KW - phenols KW - naphtols KW - aromatic amines Y1 - 1997 ER - TY - JOUR A1 - Kozhalakova, A. A. A1 - Zhubanova, Azhar A. A1 - Mansurov, Z. A. A1 - Digel, Ilya A1 - Tazhibayeva, S. M. A1 - Artmann, Gerhard A1 - Temiz Artmann, Aysegül T1 - Adsorption of bacterial lipopolysaccharides on carbonized rice shell JF - Science of Central Asia (2010) Y1 - 2010 SP - 50 EP - 54 ER - TY - CHAP A1 - Stadler, Andreas M. A1 - Embs, Jan P. A1 - Zerlin, Kay A1 - Digel, Ilya A1 - Artmann, Gerhard A1 - Zaccai, Joe A1 - Büldt, Georg T1 - Temperature transitions of hemoglobin and cytosolic water diffusion in human red blood cells : [poster] N2 - Background Hemoglobin interactions in red blood cells Hemoglobin dynamics in human red blood cells Diffusion of H2O in red blood cells KW - Erythrozyt KW - Hämoglobin KW - cytosolic water diffusion KW - hemoglobin dynamics Y1 - 2007 ER - TY - CHAP A1 - Arinkin, Vladimir A1 - Digel, Ilya T1 - Water bridge phenomenon : [abstract] N2 - One of interesting but not well known water properties is related to appearance of highly ordered structures in response to strong electrical field. In 1893 Sir William Armstrong placed a cotton thread between two wine glasses filled with chemically pure water. When high DC voltage was applied between the glasses, a connection consisting of water formed, producing a "water bridge" KW - Hydrodynamik KW - Elektrodynamik KW - Wasserbrücke KW - water bridge phenomenon Y1 - 2009 ER - TY - JOUR A1 - Artmann, Gerhard A1 - Zerlin, Kay A1 - Digel, Ilya T1 - Hemoglobin Senses Body Temperature JF - Bioengineering in Cell and Tissue Research / Artmann, Gerhard M. ; Chien, Shu (Eds.) Y1 - 2008 SN - 978-3-540-75408-4 SP - 415 EP - 447 PB - Springer CY - Berlin ER - TY - JOUR A1 - Mansurov, Z. A. A1 - Jandosov, J. M. A1 - Kerimkulova, A. R. A1 - Azat, S. A1 - Zhubanova, A. A. A1 - Digel, Ilya A1 - Savistkaya, I. S. A1 - Akimbekov, Nuraly S. A1 - Kistaubaeva, A. S. T1 - Nanostructured carbon materials for biomedical use JF - Eurasian chemico-technological journal : quarterly journal of the International Higher Education Academy of Sciences N2 - One of the priority trends of carbon nanotechnology is creation of nanocomposite systems. Such carbon nanostructured composites were produced using - raw materials based on the products of agricultural waste, such as grape stones, apricot stones, rice husk. These products have a - wide spectrum of application and can be obtained in large quantities. The Institute of Combustion Problems has carried out the work on synthesis of the nanostructured carbon sorbents for multiple applications including the field of biomedicine. The article presents the data on the synthesis and physico-chemical properties of carbonaceous sorbents using physicochemical methods of investigation: separation and purification of biomolecules; isolation of phytohormone - fusicoccin; adsorbent INGO-1 in the form of an adsorption column for blood detoxification, oral (entero) sorbent - INGO-2; the study of efferent and probiotic properties and sorption activity in regard to the lipopolysaccharide (LPS), new biocomposites - based on carbonized rice husk (CRH) and cellular microorganisms; the use of CRH in wound treatment. A new material for blood detoxication (INGO-1) has been obtained. Adsorption of p-cresyl sulfate and indoxyl sulfate has shown that active carbon adsorbent can remove clinically significant level of p-cresyl sulfate and indoxyl sulfate from human plasma. Enterosorbent INGO-2 possesses high adsorption activity in relation to Gram-negative bacteria and their endotoxins. INGO-2 slows down the growth of conditionally pathogenic microorganisms, without having a negative effect on bifido and lactobacteria. The use of enterosorbent INGO-2 for sorption therapy may provide a solution to a complex problem - detoxication of the digestive tract and normalization of the intestinal micro ecology. The immobilized probiotic called "Riso-lact" was registered at the Ministry of Health of the Republic of Kazakhstan as a biologically active food additive. The developed technology is patented and provides production of the medicine in the form of freeze-dried biomass immobilized in vials. Y1 - 2014 U6 - https://doi.org/10.18321/ectj224 SN - 1562-3920 VL - 15 (2013) IS - 3 SP - 209 EP - 217 PB - Institute of Combustion Problems CY - Almaty ER - TY - JOUR A1 - Stadler, Andreas M. A1 - Digel, Ilya A1 - Embs, Jan P. A1 - Unruh, Tobias A1 - Tehei, M. A1 - Zaccai, G. A1 - Büldt, G. A1 - Artmann, Gerhard T1 - From powder to solution : Hydration dependence of human hemoglobin dynamics correlated to body temperature JF - Biophysical Journal. 96 (2009), H. 12 Y1 - 2009 SN - 0006-3495 SP - 5073 EP - 5081 PB - Cell Press CY - Cambridge, Mass. ER - TY - JOUR A1 - Akimbekov, Nuraly S. A1 - Digel, Ilya A1 - O´Heras, C. A1 - Tastambek, K.T. A1 - Savitskaya, I.S. A1 - Ualyeva, P.S. A1 - Mansurov, Z.A. A1 - Zhubanova, A.A. T1 - Adsorption of bacterial lipopolysaccharides on carbonized rice husks obtained in the batch experiments JF - Experimental Biology N2 - The scope of this study is the measurement of endotoxin adsorption rate for carbonized rice husk. It showed good adsorption properties for LPS. During the batch experiments, several techniques were used and optimized for improving the material’s adsorption behavior. Also, with the results obtained it was possible to differentiate the materials according to their adsorption capacity and kinetic characteristics. KW - surface modification KW - adsorption KW - carbonized rice husk KW - lipopolysaccharide Y1 - 2014 SN - 1563-0218 N1 - Original in russischer Sprache VL - 60 IS - 1/2 SP - 144 EP - 148 PB - Al-Farabi Kazakh National University CY - Almaty ER - TY - JOUR A1 - Digel, Ilya A1 - Trzewik, Jürgen A1 - Demirci, Taylan A1 - Temiz Artmann, Aysegül T1 - Response of fibroblasts to cyclic mechanical stress : a proteome approach / Digel, I. ; Trzewik, J. ; Demirci, T. ; Temiz Artmann, A. ; Artmann, G. M. JF - Biomedizinische Technik. 49 (2004), H. Erg.-Bd. 2 Y1 - 2004 SN - 0932-4666 SP - 1042 EP - 1043 ER - TY - JOUR A1 - Arinkin, Vladimir A1 - Digel, Ilya A1 - Porst, Dariusz A1 - Temiz Artmann, Aysegül A1 - Artmann, Gerhard T1 - Phenotyping date palm varieties via leaflet cross-sectional imaging and artificial neural network application JF - BMC bioinformatics N2 - Background True date palms (Phoenix dactylifera L.) are impressive trees and have served as an indispensable source of food for mankind in tropical and subtropical countries for centuries. The aim of this study is to differentiate date palm tree varieties by analysing leaflet cross sections with technical/optical methods and artificial neural networks (ANN). Results Fluorescence microscopy images of leaflet cross sections have been taken from a set of five date palm tree cultivars (Hewlat al Jouf, Khlas, Nabot Soltan, Shishi, Um Raheem). After features extraction from images, the obtained data have been fed in a multilayer perceptron ANN with backpropagation learning algorithm. Conclusions Overall, an accurate result in prediction and differentiation of date palm tree cultivars was achieved with average prediction in tenfold cross-validation is 89.1% and reached 100% in one of the best ANN. Y1 - 2014 U6 - https://doi.org/10.1186/1471-2105-15-55 SN - 1471-2105 VL - 15 IS - 55 SP - 1 EP - 8 ER - TY - JOUR A1 - Preiß, C. A1 - Linder, Peter A1 - Wendt, K. A1 - Krystek, M. A1 - Digel, Ilya A1 - Gossmann, Matthias A1 - Temiz Artmann, Aysegül A1 - Porst, Dariusz A1 - Kayser, Peter A1 - Bassam, Rasha A1 - Artmann, Gerhard T1 - Engineering technology for plant physiology and plant stress research N2 - Plant physiology and plant stress: Plant physiology will be much more important for human mankind because of yield and cultivation limits of crops determined by their resistance to stress. To assess and counteract various stress factors it is necessary to conduct plant research to gain information and results on plant physiology. KW - Pflanzenphysiologie KW - Pflanzenstress KW - Pflanzenscanner KW - plant stress KW - plant scanner Y1 - 2011 ER - TY - CHAP A1 - de Honde, Lukas A1 - Porst, Dariusz A1 - Digel, Ilya ED - Fischerauer, Alice T1 - A randomized, observational thermographic study of the neck region before and after a physiotherapeutic intervention T2 - 2nd YRA MedTech Symposium 2017 : June 8th - 9th / 2017 / Hochschule Ruhr-West Y1 - 2017 SN - 978-3-9814801-9-1 U6 - https://doi.org/10.17185/duepublico/43984 N1 - A young researchers track of the 7th IEEE Workshop & SENSORICA 2017 SP - 122 EP - 123 PB - Universität Duisburg-Essen CY - Duisburg ER - TY - CHAP A1 - Azar, Fouad A1 - Digel, Ilya ED - Staat, Manfred ED - Erni, Daniel T1 - Utilization of fluorescence spectroscopy and neural networks in clinical analysis T2 - 3rd YRA MedTech Symposium 2019 : May 24 / 2019 / FH Aachen N2 - Fluorescence topography of human urine in combination with learning algorithms can provide a variant pattern recognition method in analytical clinical chemistry and, eventually, diagnosis. Y1 - 2019 SN - 978-3-940402-22-6 U6 - https://doi.org/10.17185/duepublico/48750 SP - 40 EP - 41 PB - Universität Duisburg-Essen CY - Duisburg ER - TY - JOUR A1 - Artmann, Gerhard A1 - Digel, Ilya A1 - Zerlin, Kay A1 - Maggakis-Kelemen, Christina A1 - Linder, Peter A1 - Porst, Dariusz A1 - Kayser, Peter A1 - Stadler, David A1 - Dikta, Gerhard A1 - Temiz Artmann, Aysegül T1 - Hemoglobin senses body temperature JF - European Biophysics Journal Y1 - 2009 SN - 0175-7571 VL - 38 IS - 5 SP - 589 EP - 600 ER - TY - JOUR A1 - Kowalski, Julia A1 - Linder, Peter A1 - Zierke, S. A1 - Wulfen, B. van A1 - Clemens, J. A1 - Konstantinidis, K. A1 - Ameres, G. A1 - Hoffmann, R. A1 - Mikucki, J. A1 - Tulaczyk, S. A1 - Funke, O. A1 - Blandfort, D. A1 - Espe, Clemens A1 - Feldmann, Marco A1 - Francke, Gero A1 - Hiecker, S. A1 - Plescher, Engelbert A1 - Schöngarth, Sarah A1 - Dachwald, Bernd A1 - Digel, Ilya A1 - Artmann, Gerhard A1 - Eliseev, D. A1 - Heinen, D. A1 - Scholz, F. A1 - Wiebusch, C. A1 - Macht, S. A1 - Bestmann, U. A1 - Reineking, T. A1 - Zetzsche, C. A1 - Schill, K. A1 - Förstner, R. A1 - Niedermeier, H. A1 - Szumski, A. A1 - Eissfeller, B. A1 - Naumann, U. A1 - Helbing, K. T1 - Navigation technology for exploration of glacier ice with maneuverable melting probes JF - Cold Regions Science and Technology N2 - The Saturnian moon Enceladus with its extensive water bodies underneath a thick ice sheet cover is a potential candidate for extraterrestrial life. Direct exploration of such extraterrestrial aquatic ecosystems requires advanced access and sampling technologies with a high level of autonomy. A new technological approach has been developed as part of the collaborative research project Enceladus Explorer (EnEx). The concept is based upon a minimally invasive melting probe called the IceMole. The force-regulated, heater-controlled IceMole is able to travel along a curved trajectory as well as upwards. Hence, it allows maneuvers which may be necessary for obstacle avoidance or target selection. Maneuverability, however, necessitates a sophisticated on-board navigation system capable of autonomous operations. The development of such a navigational system has been the focal part of the EnEx project. The original IceMole has been further developed to include relative positioning based on in-ice attitude determination, acoustic positioning, ultrasonic obstacle and target detection integrated through a high-level sensor fusion. This paper describes the EnEx technology and discusses implications for an actual extraterrestrial mission concept. Y1 - 2016 U6 - https://doi.org/10.1016/j.coldregions.2015.11.006 SN - 0165-232X IS - 123 SP - 53 EP - 70 PB - Elsevier CY - Amsterdam ER -