TY  - JOUR
A1  - Mikucki, Jill Ann
A1  - Schuler, C. G.
A1  - Digel, Ilya
A1  - Kowalski, Julia
A1  - Tuttle, M. J.
A1  - Chua, Michelle
A1  - Davis, R.
A1  - Purcell, Alicia
A1  - Ghosh, D.
A1  - Francke, G.
A1  - Feldmann, M.
A1  - Espe, C.
A1  - Heinen, Dirk
A1  - Dachwald, Bernd
A1  - Clemens, Joachim
A1  - Lyons, W. B.
A1  - Tulaczyk, S.
T1  - Field-Based planetary protection operations for melt probes: validation of clean access into the blood falls, antarctica, englacial ecosystem
JF  - Astrobiology
N2  - Subglacial environments on Earth offer important analogs to Ocean World targets in our solar system. These unique microbial ecosystems remain understudied due to the challenges of access through thick glacial ice (tens to hundreds of meters). Additionally, sub-ice collections must be conducted in a clean manner to ensure sample integrity for downstream microbiological and geochemical analyses. We describe the field-based cleaning of a melt probe that was used to collect brine samples from within a glacier conduit at Blood Falls, Antarctica, for geomicrobiological studies. We used a thermoelectric melting probe called the IceMole that was designed to be minimally invasive in that the logistical requirements in support of drilling operations were small and the probe could be cleaned, even in a remote field setting, so as to minimize potential contamination. In our study, the exterior bioburden on the IceMole was reduced to levels measured in most clean rooms, and below that of the ice surrounding our sampling target. Potential microbial contaminants were identified during the cleaning process; however, very few were detected in the final englacial sample collected with the IceMole and were present in extremely low abundances (∼0.063% of 16S rRNA gene amplicon sequences). This cleaning protocol can help minimize contamination when working in remote field locations, support microbiological sampling of terrestrial subglacial environments using melting probes, and help inform planetary protection challenges for Ocean World analog mission concepts.
Y1  - 2023
U6  - https://doi.org/10.1089/ast.2021.0102
SN  - 1557-8070 (online)
SN  - 153-1074 (print)
VL  - 23
IS  - 11
SP  - 1165
EP  - 1178
PB  - Liebert
CY  - New York, NY
ER  - 
TY  - JOUR
A1  - Akimbekov, Nuraly S.
A1  - Digel, Ilya
A1  - Tastambek, Kuanysh T.
A1  - Kozhahmetova, Marzhan
A1  - Sherelkhan, Dinara K.
A1  - Tauanov, Zhandos
T1  - Hydrogenotrophic methanogenesis in coal-bearing environments: Methane production, carbon sequestration, and hydrogen availability
JF  - International Journal of Hydrogen Energy
N2  - Methane is a valuable energy source helping to mitigate the growing energy demand worldwide. However, as a potent greenhouse gas, it has also gained additional attention due to its environmental impacts. The biological production of methane is performed primarily hydrogenotrophically from H2 and CO2 by methanogenic archaea. Hydrogenotrophic methanogenesis also represents a great interest with respect to carbon re-cycling and H2 storage. The most significant carbon source, extremely rich in complex organic matter for microbial degradation and biogenic methane production, is coal. Although interest in enhanced microbial coalbed methane production is continuously increasing globally, limited knowledge exists regarding the exact origins of the coalbed methane and the associated microbial communities, including hydrogenotrophic methanogens. Here, we give an overview of hydrogenotrophic methanogens in coal beds and related environments in terms of their energy production mechanisms, unique metabolic pathways, and associated ecological functions.
KW  - Coal
KW  - Methanogenesis
KW  - Methane
KW  - Hydrogenotrophic methanogens
KW  - H2
Y1  - 2024
U6  - https://doi.org/10.1016/j.ijhydene.2023.09.223
SN  - 1879-3487 (online)
SN  - 0360-3199 (print)
VL  - 52
IS  - Part D
SP  - 1264
EP  - 1277
PB  - Elsevier
CY  - New York
ER  - 
TY  - JOUR
A1  - Temiz Artmann, Aysegül
A1  - Linder, Peter
A1  - Kayser, Peter
A1  - Digel, Ilya
T1  - NMR in vitro effects on proliferation, apoptosis, and viability of human chondrocytes and osteoblasts
JF  - Methods and findings in Experimental and Clinical Pharmacology. 27 (2005), H. 6
Y1  - 2005
SN  - 0379-0355
SP  - 391
EP  - 394
ER  - 
TY  - BOOK
A1  - Digel, Ilya
T1  - Enhancement of microbial adhesion onto solid surfaces using transition metals and water-soluble polymers : academic PhD dissertation in microbiology, Division of Applied Microbiology, Department of Microbiology, Biology Faculty, Kazakh National State University, Almaty, Kazakhstan / Ilya Digel
Y1  - 1998
CY  - Almaty
ER  - 
TY  - JOUR
A1  - Bogoyavlenskiy, A. P.
A1  - Berezin, V. E.
A1  - Ogneva, A. V.
A1  - Tolmacheva, V. P.
A1  - Digel, Ilya
A1  - Khudyakova, S. S.
T1  - Immunostimulating activity of a saponin-containing extract of Saponaria officinalis
JF  - Voprosy virusologii
Y1  - 1999
SN  - 0507-4088
VL  - 44
IS  - 5
SP  - 229
EP  - 232
ER  - 
TY  - CHAP
A1  - Schneider, Oliver
A1  - Al Hakim, Taher
A1  - Kayser, Peter
A1  - Digel, Ilya
ED  - Erni, Daniel
ED  - Fischerauer, Alice
ED  - Himmel, Jörg
ED  - Seeger, Thomas
ED  - Thelen, Klaus
T1  - Development and trials of a test chamber for ultrasound-assisted sampling of living cells from solid surfaces
T2  - 2nd YRA MedTech Symposium 2017 : June 8th - 9th / 2017 / Hochschule Ruhr-West
Y1  - 2017
SN  - 978-3-9814801-9-1
U6  - https://doi.org/10.17185/duepublico/43984
N1  - A young researchers track of the 7th IEEE Workshop & SENSORICA 2017
SP  - 96
EP  - 97
PB  - Universität Duisburg-Essen
CY  - Duisburg
ER  -