TY  - JOUR
A1  - Werner, Frederik
A1  - Krumbe, Christoph
A1  - Schumacher, Katharina
A1  - Groebel, Simone
A1  - Spelthahn, Heiko
A1  - Stellberg, Michael
A1  - Wagner, Torsten
A1  - Yoshinobu, Tatsuo
A1  - Selmer, Thorsten
A1  - Keusgen, Michael
A1  - Baumann, Marcus
A1  - Schöning, Michael Josef
T1  - Determination of the extracellular acidification of Escherichia coli by a light-addressable potentiometric sensor
JF  - Physica status solidi (a) : applications and material science. 208 (2011), H. 6
Y1  - 2011
SN  - 1862-6319
SP  - 1340
EP  - 1344
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Werner, Frederik
A1  - Groebel, Simone
A1  - Krumbe, Christoph
A1  - Wagner, Torsten
A1  - Selmer, Thorsten
A1  - Yoshinobu, Tatsuo
A1  - Baumann, Marcus
A1  - Schöning, Michael Josef
T1  - Nutrient concentration-sensitive microorganism-based biosensor
JF  - Physica Status Solidi (a)
Y1  - 2012
U6  - http://dx.doi.org/10.1002/pssa.201100801
SN  - 1862-6319
VL  - 209
IS  - 5
SP  - 900
EP  - 904
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Schiffels, Johannes
A1  - Pinkenburg, Olaf
A1  - Schelden, Maximilian
A1  - Aboulnaga, El-Hussiny A. A.
A1  - Baumann, Marcus
A1  - Selmer, Thorsten
T1  - An innovative cloning platform enables large-scale production and maturation of an oxygen-tolerant [NiFe]-hydrogenase from cupriavidus necator in Escherichia coli
JF  - PLOS one. 2013
Y1  - 2013
U6  - http://dx.doi.org/10.1371/journal.pone.0068812
SN  - 1932-6203
PB  - Public Library of Science
CY  - San Francisco, California
ER  - 
TY  - JOUR
A1  - Huck, Christina
A1  - Schiffels, Johannes
A1  - Herrera, Cony N.
A1  - Schelden, Maximilian
A1  - Selmer, Thorsten
A1  - Poghossian, Arshak
A1  - Baumann, Marcus
A1  - Wagner, Patrick
A1  - Schöning, Michael Josef
T1  - Metabolic responses of Escherichia coli upon glucose pulses captured by a capacitive field-effect sensor
JF  - Physica Status Solidi (A)
N2  - Living cells are complex biological systems transforming metabolites taken up from the surrounding medium. Monitoring the responses of such cells to certain substrate concentrations is a challenging task and offers possibilities to gain insight into the vitality of a community influenced by the growth environment. Cell-based sensors represent a promising platform for monitoring the metabolic activity and thus, the “welfare” of relevant organisms. In the present study, metabolic responses of the model bacterium Escherichia coli in suspension, layered onto a capacitive field-effect structure, were examined to pulses of glucose in the concentration range between 0.05 and 2 mM. It was found that acidification of the surrounding medium takes place immediately after glucose addition and follows Michaelis–Menten kinetic behavior as a function of the glucose concentration. In future, the presented setup can, therefore, be used to study substrate specificities on the enzymatic level and may as well be used to perform investigations of more complex metabolic responses. Conclusions and perspectives highlighting this system are discussed.
Y1  - 2013
U6  - http://dx.doi.org/10.1002/pssa.201200900
SN  - 0031-8965
VL  - 210
IS  - 5
SP  - 926
EP  - 931
PB  - Wiley-VCH
CY  - Weinheim
ER  -