TY  - JOUR
A1  - Özsoylu, Dua
A1  - Aliazizi, Fereshteh
A1  - Wagner, Patrick
A1  - Schöning, Michael Josef
T1  - Template bacteria-free fabrication of surface imprinted polymer-based biosensor for E. coli detection using photolithographic mimics: Hacking bacterial adhesion
JF  - Biosensors and Bioelectronics
N2  - As one class of molecular imprinted polymers (MIPs), surface imprinted polymer (SIP)-based biosensors show great potential in direct whole-bacteria detection. Micro-contact imprinting, that involves stamping the template bacteria immobilized on a substrate into a pre-polymerized polymer matrix, is the most straightforward and prominent method to obtain SIP-based biosensors. However, the major drawbacks of the method arise from the requirement for fresh template bacteria and often non-reproducible bacteria distribution on the stamp substrate. Herein, we developed a positive master stamp containing photolithographic mimics of the template bacteria (E. coli) enabling reproducible fabrication of biomimetic SIP-based biosensors without the need for the “real” bacteria cells. By using atomic force and scanning electron microscopy imaging techniques, respectively, the E. coli-capturing ability of the SIP samples was tested, and compared with non-imprinted polymer (NIP)-based samples and control SIP samples, in which the cavity geometry does not match with E. coli cells. It was revealed that the presence of the biomimetic E. coli imprints with a specifically designed geometry increases the sensor E. coli-capturing ability by an “imprinting factor” of about 3. These findings show the importance of geometry-guided physical recognition in bacterial detection using SIP-based biosensors. In addition, this imprinting strategy was employed to interdigitated electrodes and QCM (quartz crystal microbalance) chips. E. coli detection performance of the sensors was demonstrated with electrochemical impedance spectroscopy (EIS) and QCM measurements with dissipation monitoring technique (QCM-D).
KW  - Surface imprinted polymer
KW  - E. coli detection
KW  - Photolithographic mimics
KW  - Master stamp
KW  - Quartz crystal microbalance
Y1  - 2024
U6  - https://doi.org/10.1016/j.bios.2024.116491
SN  - 1873-4235 (eISSN)
SN  - 0956-5663
N1  - Corresponding author: Michael J. Schöning
VL  - 261
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Muschallik, Lukas
A1  - Molinnus, Denise
A1  - Bongaerts, Johannes
A1  - Pohl, Martina
A1  - Wagner, Torsten
A1  - Schöning, Michael Josef
A1  - Siegert, Petra
A1  - Selmer, Thorsten
T1  - (R,R)-Butane-2,3-diol Dehydrogenase from Bacillus clausii DSM 8716T: Cloning and Expression of the bdhA-Gene, and Initial Characterization of Enzyme
JF  - Journal of Biotechnology
N2  - The gene encoding a putative (R,R)-butane-2,3-diol dehydrogenase (bdhA) from Bacillus clausii DSM 8716T was isolated, sequenced and expressed in Escherichia coli. The amino acid sequence of the encoded protein is only distantly related to previously studied enzymes (identity 33–43%) and exhibited some uncharted peculiarities. An N-terminally StrepII-tagged enzyme variant was purified and initially characterized. The isolated enzyme catalyzed the (R)-specific oxidation of (R,R)- and meso-butane-2,3-diol to (R)- and (S)-acetoin with specific activities of 12 U/mg and 23 U/mg, respectively. Likewise, racemic acetoin was reduced with a specific activity of up to 115 U/mg yielding a mixture of (R,R)- and meso-butane-2,3-diol, while the enzyme reduced butane-2,3-dione (Vmax 74 U/mg) solely to (R,R)-butane-2,3-diol via (R)-acetoin. For these reactions only activity with the co-substrates NADH/NAD+ was observed. The enzyme accepted a selection of vicinal diketones, α-hydroxy ketones and vicinal diols as alternative substrates. Although the physiological function of the enzyme in B. clausii remains elusive, the data presented herein clearly demonstrates that the encoded enzyme is a genuine (R,R)-butane-2,3-diol dehydrogenase with potential for applications in biocatalysis and sensor development.
Y1  - 2017
U6  - https://doi.org/10.1016/j.jbiotec.2017.07.020
SN  - 0168-1656
VL  - 258
SP  - 41
EP  - 50
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - CHAP
A1  - Bäcker, Matthias
A1  - Koch, C.
A1  - Geiger, F.
A1  - Eber, F.
A1  - Gliemann, H.
A1  - Poghossian, Arshak
A1  - Schöning, Michael Josef
T1  - A New Class of Biosensors Based on Tobacco Mosaic Virus and Coat Proteins as Enzyme Nanocarrier
T2  - Procedia Engineering
Y1  - 2016
U6  - https://doi.org/10.1016/j.proeng.2016.11.228
SN  - 1877-7058
N1  - Proceedings of the 30th anniversary Eurosensors Conference – Eurosensors 2016, 4-7. Sepember 2016, Budapest, Hungary
VL  - Vol. 168
SP  - 618
EP  - 621
ER  - 
TY  - CHAP
A1  - Wu, Chunsheng
A1  - Poghossian, Arshak
A1  - Werner, Frederik
A1  - Bronder, Thomas
A1  - Bäcker, Matthias
A1  - Wang, Ping
A1  - Schöning, Michael Josef
T1  - An application of a scanning light-addressable potentiometric sensor for label-free DNA detection
T2  - 11. Dresdner Sensor-Symposium : 9.-11.12.2013
Y1  - 2013
SN  - 978-3-9813484-5-3
SP  - 164
EP  - 168
ER  - 
TY  - JOUR
A1  - Bronder, Thomas
A1  - Poghossian, Arshak
A1  - Scheja, S.
A1  - Wu, Chunsheng
A1  - Keusgen, M.
A1  - Schöning, Michael Josef
T1  - Electrostatic Detection of Unlabelled Single- and Double-stranded DNA Using Capacitive Field-effect Devices Functionalized with a Positively Charged Polyelectrolyte Layer
JF  - Procedia Engineering
N2  - Capacitive field-effect electrolyte-insulator-semiconductor sensors consisting of an Al-p-Si-SiO2 structure have been used for the electrical detection of unlabelled single- and double-stranded DNA (dsDNA) molecules by their intrinsic charge. A simple functionalization protocol based on the layer-by-layer (LbL) technique was used to prepare a weak polyelectrolyte/probe-DNA bilayer, followed by the hybridization with complementary target DNA molecules. Due to the flat orientation of the LbL-adsorbed DNA molecules, a high sensor signal has been achieved. In addition, direct label-free detection of in-solution hybridized dsDNA molecules has been studied.
Y1  - 2015
U6  - https://doi.org/10.1016/j.proeng.2015.08.710
SN  - 1877-7058
N1  - Eurosensors 2015
VL  - 120
SP  - 544
EP  - 547
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Poghossian, Arshak
A1  - Ingebrandt, S.
A1  - Abouzar, Maryam H.
A1  - Schöning, Michael Josef
T1  - Label-free detection of charged macromolecules by using a field-effect-based sensor platform: Experiments and possible mechanisms of signal generation
JF  - Applied Physics A: Materials Science & Processing. 87 (2007), H. 3
Y1  - 2007
SN  - 0947-8396
N1  - Special Issue “From Surface Science to Nanoscale Devices”
SP  - 517
EP  - 524
ER  - 
TY  - JOUR
A1  - Poghossian, Arshak
A1  - Katz, Evgeny
A1  - Schöning, Michael Josef
T1  - Enzyme logic AND-Reset and OR-Reset gates based on a field-effect electronic transducer modified with multi-enzyme membrane
JF  - Chemical Communications
N2  - Capacitive field-effect sensors modified with a multi-enzyme membrane have been applied for an electronic transduction of biochemical signals processed by enzyme-based AND-Reset and OR-Reset logic gates. The local pH change at the sensor surface induced by the enzymatic reaction was used for the activation of the Reset function for the first time.
Y1  - 2015
U6  - https://doi.org/10.1039/C5CC01362C
VL  - 51
SP  - 6564
EP  - 6567
PB  - Royal Society of Chemistry (RSC)
CY  - Cambridge
ER  - 
TY  - JOUR
A1  - Abouzar, Maryam H.
A1  - Poghossian, Arshak
A1  - Razavi, A.
A1  - Williams, O. A.
A1  - Bijnens, N.
A1  - Wagner, P.
A1  - Schöning, Michael Josef
T1  - Characterisation of capacitive field-effect sensors with a nanocrystalline-diamond film as transducer material for multi-parameter sensing
JF  - Biosensors and Bioelectronics. 24 (2009), H. 5
Y1  - 2009
SN  - 0956-5663
N1  - Selected Papers from the Tenth World Congress on Biosensors Shangai, China, May 14-16, 2008 ; Zeitschrift früher u.d.T. : Biosensors
SP  - 1298
EP  - 1304
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - CHAP
A1  - Schöning, Michael Josef
A1  - Abouzar, Maryam H.
A1  - Wagner, Torsten
A1  - Näther, Niko
A1  - Rolka, David
A1  - Yoshinobu, Tatsuo
A1  - Kloock, Joachim P.
A1  - Turek, Monika
A1  - Ingebrandt, Sven
A1  - Poghossian, Arshak
T1  - A semiconductor-based field-effect platform for (bio-)chemical and physical sensors: From capacitive EIS sensors and LAPS over ISFETs to nano-scale devices
T2  - MRS Proceedings
Y1  - 2006
U6  - https://doi.org/10.1557/PROC-0952-F08-02
N1  - Vol. 952 - Symposium F - Integrated Nanosensors
SP  - 1
EP  - 9
ER  - 
TY  - JOUR
A1  - Turek, M.
A1  - Keusgen, M.
A1  - Poghossian, Arshak
A1  - Mulchandani, A.
A1  - Wang, J.
A1  - Schöning, Michael Josef
T1  - Enzyme-modified electrolyte-insulator-semiconductor sensors
JF  - Journal of Contemporary Physics. 43 (2008), H. 2
Y1  - 2008
SN  - 1934-9378
N1  - Armenian Academy of Sciences
SP  - 82
EP  - 85
ER  - 
TY  - JOUR
A1  - Poghossian, Arshak
A1  - Ingebrandt, S.
A1  - Yeung, C.-K.
A1  - Offenhäusser, A.
A1  - Schöning, Michael Josef
T1  - Microsensors based on ion-sensitive field-effect transistors for biomedical applications
JF  - Biomedizinische Technik. 49 (2004), H. 2
Y1  - 2004
SN  - 0932-4666
SP  - 1036
EP  - 1037
ER  - 
TY  - JOUR
A1  - Bäcker, Matthias
A1  - Beging, Stefan
A1  - Biselli, Manfred
A1  - Poghossian, Arshak
A1  - Wang, J.
A1  - Zang, Werner
A1  - Wagner, Patrick
A1  - Schöning, Michael Josef
T1  - Concept for a solid-state multi-parameter sensor system for cell-culture monitoring
JF  - Electrochimica Acta. 54 (2009), H. 25 Sp. Iss. SI
Y1  - 2009
SN  - 0013-4686
SP  - 6107
EP  - 6112
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Buniatyan, Vahe V.
A1  - Matirosyan, N.
A1  - Abouzar, Maryam H.
A1  - Schubert, J.
A1  - Zander, W.
A1  - Gevorgian, S.
A1  - Schöning, Michael Josef
A1  - Poghossian, Arshak
T1  - Capacitive field-effect pH sensor based on an electrolyte-ferroelectric-insulator-semiconductor structure
JF  - SENSOR 2009 : : 14th International Conference on Sensors, Technologies, Electronics and Applications; Nürnberg, Germany, 26 - 28 May 2009; proceedings; [part of] Sensor + Test Conference 2009 / AMA, Fachverband für Sensorik e.V
Y1  - 2009
SN  - 9783981099355
N1  - AMA, Fachverband für Sensorik ; Sensor International Conference ; (14 : ; 2009.05.26-28 : ; Nürnberg)
SP  - 317
EP  - 322
PB  - AMA Service
CY  - Wunstorf
ER  - 
TY  - JOUR
A1  - Poghossian, Arshak
A1  - Berndsen, Lars
A1  - Schöning, Michael Josef
T1  - Chemical sensor as physical sensor: ISFET-based flowvelocity, flow-direction and diffusion-coefficient sensor
JF  - Sensors and Actuators B. 95 (2003), H. 1-3
Y1  - 2003
SN  - 0925-4005
SP  - 384
EP  - 390
ER  - 
TY  - JOUR
A1  - Poghossian, Arshak
A1  - Wagner, Holger
A1  - Schöning, Michael Josef
T1  - Functional testing and characterisation of (bio-)chemical sensors on wafer level
JF  - Sensors and Actuators B: Chemical. 154 (2011), H. 2
Y1  - 2011
SN  - 1873-3077
N1  - EUROSENSORS XXIII
SP  - 169
EP  - 173
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Poghossian, Arshak
A1  - Berndsen, L.
A1  - Schultze, J. W.
A1  - Lüth, H.
A1  - Schöning, Michael Josef
T1  - „High order“ hybrid sensor module based on an identical transducer principle
JF  - Chemical and biological sensors and analytical methods : proceedings of the international symposium / Sensor, Physical Electrochemistry, and Organic and Biological Electrochemistry Divisions. Ed.: M. Butler
Y1  - 2001
SN  - 1-56677-351-2
N1  - International Symposium: Chemical and Biological Sensors and Analytical Methods ; (2 : 2001.)
SP  - 143
EP  - 152
PB  - Electrochemical Society
CY  - Pennington, NJ
ER  - 
TY  - JOUR
A1  - Poghossian, Arshak
A1  - Thust, M.
A1  - Schroth, P.
A1  - Steffen, A.
A1  - Lüth, H.
A1  - Schöning, Michael Josef
T1  - Penicillin detection by means of silicon-based field-effect structures
JF  - Sensors and Materials. 13 (2001), H. 4
Y1  - 2001
SN  - 0392-2510
SP  - 207
EP  - 223
ER  - 
TY  - JOUR
A1  - Bäcker, Matthias
A1  - Poghossian, Arshak
A1  - Abouzar, Maryam H.
A1  - Wenmackers, Sylvia
A1  - Janssens, Stoffel D.
A1  - Haenen, Ken
A1  - Wagner, Patrick
A1  - Schöning, Michael Josef
T1  - Capacitive field-effect (bio-)chemical sensors based on nanocrystalline diamond films
JF  - Diamond Electronics and Bioelectronics — Fundamentals to Applications III, edited by P. Bergonzo, [u.a.]
Y1  - 2010
N1  - MRS Proceedings Volume 1203 paper 1203-J17-31 ; Mater. Res. Soc. Sympos. Proc. Vol 1203 (2010) ; Materials Research Society
SP  - 1
EP  - 6
ER  - 
TY  - CHAP
A1  - Weil, M.
A1  - Poghossian, Arshak
A1  - Schöning, Michael Josef
A1  - Cherstvy, A.
T1  - Electrical monitoring of layer-by-layer adsorption of oppositely charged macromolecules by means of capacitive field-effect devices
Y1  - 2012
SN  - 978-3-9813484-2-2
U6  - https://doi.org/10.5162/IMCS2012/P2.5.2
SP  - 1575
EP  - 1578
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Näther, Niko
A1  - Auger, V.
A1  - Poghossian, Arshak
A1  - Koudelka-Hep, M.
T1  - Miniaturised flow-through cell with integrated capacitive EIS sensor fabricated at wafer level using Si and SU-8 technologies
JF  - Sensors and Actuators B. 108 (2005), H. 1-2
Y1  - 2005
SN  - 0925-4005
N1  - Proceedings of the Tenth International Meeting on Chemical Sensors — IMCS - 10 2004
SP  - 986
EP  - 992
ER  - 
TY  - JOUR
A1  - Poghossian, Arshak
A1  - Schöning, Michael Josef
T1  - Recent progress in silicon-based biologically sensitive field-effect devices
JF  - Current Opinion in Electrochemistry
N2  - Biologically sensitive field-effect devices (BioFEDs) advantageously combine the electronic field-effect functionality with the (bio)chemical receptor’s recognition ability for (bio)chemical sensing. In this review, basic and widely applied device concepts of silicon-based BioFEDs (ion-sensitive field-effect transistor, silicon nanowire transistor, electrolyte-insulator-semiconductor capacitor, light-addressable potentiometric sensor) are presented and recent progress (from 2019 to early 2021) is discussed. One of the main advantages of BioFEDs is the label-free sensing principle enabling to detect a large variety of biomolecules and bioparticles by their intrinsic charge. The review encompasses applications of BioFEDs for the label-free electrical detection of clinically relevant protein biomarkers, deoxyribonucleic acid molecules and viruses, enzyme-substrate reactions as well as recording of the cell acidification rate (as an indicator of cellular metabolism) and the extracellular potential.
Y1  - 2021
U6  - https://doi.org/10.1016/j.coelec.2021.100811
SN  - 2451-9103
IS  - Article number: 100811
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Poghossian, Arshak
A1  - Schöning, Michael Josef
A1  - Schroth, P.
A1  - Simonis, A.
A1  - Lüth, H.
T1  - An ISFET-based penicillin sensor with high sensitivity, low detection limit and long lifetime
JF  - Sensors and Actuators B. 76 (2001), H. 1-3
Y1  - 2001
SN  - 0925-4005
SP  - 519
EP  - 526
ER  - 
TY  - JOUR
A1  - Huck, Christina
A1  - Schiffels, Johannes
A1  - Herrera, Cony N.
A1  - Schelden, Maximilian
A1  - Selmer, Thorsten
A1  - Poghossian, Arshak
A1  - Baumann, Marcus
A1  - Wagner, Patrick
A1  - Schöning, Michael Josef
T1  - Metabolic responses of Escherichia coli upon glucose pulses captured by a capacitive field-effect sensor
JF  - Physica Status Solidi (A)
N2  - Living cells are complex biological systems transforming metabolites taken up from the surrounding medium. Monitoring the responses of such cells to certain substrate concentrations is a challenging task and offers possibilities to gain insight into the vitality of a community influenced by the growth environment. Cell-based sensors represent a promising platform for monitoring the metabolic activity and thus, the “welfare” of relevant organisms. In the present study, metabolic responses of the model bacterium Escherichia coli in suspension, layered onto a capacitive field-effect structure, were examined to pulses of glucose in the concentration range between 0.05 and 2 mM. It was found that acidification of the surrounding medium takes place immediately after glucose addition and follows Michaelis–Menten kinetic behavior as a function of the glucose concentration. In future, the presented setup can, therefore, be used to study substrate specificities on the enzymatic level and may as well be used to perform investigations of more complex metabolic responses. Conclusions and perspectives highlighting this system are discussed.
Y1  - 2013
U6  - https://doi.org/10.1002/pssa.201200900
SN  - 0031-8965
VL  - 210
IS  - 5
SP  - 926
EP  - 931
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - CHAP
A1  - Poghossian, Arshak
A1  - Bronder, Thomas
A1  - Scheja, S.
A1  - Wu, Chunsheng
A1  - Metzger-Boddien, C.
A1  - Keusgen, M.
A1  - Schöning, Michael Josef
T1  - Label-free Electrostatic Detection of DNA Amplification by PCR Using Capacitive Field-effect Devices
T2  - Procedia Engineering
N2  - A capacitive field-effect EIS (electrolyte-insulator-semiconductor) sensor modified with a positively charged weak polyelectrolyte of poly(allylamine hydrochloride) (PAH)/single-stranded probe DNA (ssDNA) bilayer has been used for a label-free electrostatic detection of pathogen-specific DNA amplification via polymerase chain reaction (PCR). The sensor is able to distinguish between positive and negative PCR solutions, to detect the existence of target DNA amplicons in PCR samples and thus, can be used as tool for a quick verification of DNA amplification and the successful PCR process.
Y1  - 2016
U6  - https://doi.org/10.1016/j.proeng.2016.11.512
SN  - 1877-7058
N1  - Proceedings of the 30th anniversary Eurosensors Conference – Eurosensors 2016, 4-7. Sepember 2016, Budapest, Hungary
VL  - Vol. 168
SP  - 514
EP  - 517
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Siqueira, Jose R.
A1  - Werner, Frederik
A1  - Bäcker, Matthias
A1  - Poghossian, Arshak
A1  - Zucolotto, Valtencir
A1  - Oliveira, Osvaldo N. Jr.
A1  - Schöning, Michael Josef
T1  - Layer-by-Layer Assembly of Carbon Nanotubes Incorporated in Light-Addressable Potentiometric Sensors
JF  - Journal of Physical Chemistry C. 113 (2009), H. 33
Y1  - 2009
SN  - 1932-7455
SP  - 14765
EP  - 14770
PB  - American Chemical Society
CY  - Washington, DC
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Brinkmann, D.
A1  - Rolka, David
A1  - Demuth, C.
A1  - Poghossian, Arshak
T1  - CIP (cleaning-in-place) suitable “non-glass” pH sensor based on a Ta2O5-gate EIS structure
JF  - Sensors and Actuators B: Chemical. 111-112 (2005)
Y1  - 2005
SN  - 0925-4005
N1  - Eurosensors XVIII 2004 - The 18th European Conference on Solid-State Transducers
SP  - 423
EP  - 429
ER  - 
TY  - JOUR
A1  - Poghossian, Arshak
A1  - Werner, Frederik
A1  - Buniatyan, V. V.
A1  - Wagner, Torsten
A1  - Miamoto, K.
A1  - Yoshinobu, T.
A1  - Schöning, Michael Josef
T1  - Towards addressability of light-addressable potentiometric sensors: Shunting effect of non-illuminated region and cross-talk
JF  - Sensor and Actuators B: Chemical
N2  - The LAPS (light-addressable potentiometric sensor) platform is one of the most attractive approaches for chemical and biological sensing with many applications ranging from pH and ion/analyte concentration measurements up to cell metabolism detection and chemical imaging. However, although it is generally accepted that LAPS measurements are spatially resolved, the light-addressability feature of LAPS devices has not been discussed in detail so far. In this work, an extended electrical equivalent-circuit model of the LAPS has been presented, which takes into account possible cross-talk effects due to the capacitive coupling of the non-illuminated region. A shunting effect of the non-illuminated area on the measured photocurrent and addressability of LAPS devices has been studied. It has been shown, that the measured photocurrent will be determined not only by the local interfacial potential in the illuminated region but also by possible interfacial potential changes in the non-illuminated region, yielding cross-talk effects. These findings were supported by the experimental investigations of a penicillin-sensitive multi-spot LAPS and a metal-insulator-semiconductor LAPS as model systems.
Y1  - 2017
U6  - https://doi.org/10.1016/j.snb.2017.01.047
SN  - 0925-4005
IS  - 244
SP  - 1071
EP  - 1079
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Poghossian, Arshak
T1  - Detection of charged macromolecules by means of field-effect devices (FEDs): possibilities and limitations
JF  - Electrochemical sensors, biosensors and their biomedical applications / ed. by Xueji Zhang ...
Y1  - 2008
SN  - 978-0-12-373738-0
SP  - 187
EP  - 212
PB  - Elsevier Acad. Press
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Christiaens, P.
A1  - Abouzar, Maryam H.
A1  - Poghossian, Arshak
A1  - Wagner, Torsten
A1  - Bijnens, N.
A1  - Williams, O. A.
A1  - Daenen, M.
A1  - Haenen, K.
A1  - Douthéret, O.
A1  - Haen, J. d´
A1  - Mekhalif, Z.
A1  - Schöning, Michael Josef
A1  - Wagner, P.
T1  - pH sensitivity of nanocrystalline diamond films
JF  - Physica status solidi (A). 204 (2007), H. 9
Y1  - 2007
SN  - 0031-8965
SP  - 2925
EP  - 2930
ER  - 
TY  - JOUR
A1  - Wu, Chunsheng
A1  - Poghossian, Arshak
A1  - Bronder, Thomas
A1  - Schöning, Michael Josef
T1  - Sensing of double-stranded DNA molecules by their intrinsic molecular charge using the light-addressable potentiometric sensor
JF  - Sensors and Actuators B: Chemical
N2  - A multi-spot light-addressable potentiometric sensor (LAPS), which belongs to the family of semiconductor field-effect devices, was applied for label-free detection of double-stranded deoxyribonucleic acid (dsDNA) molecules by their intrinsic molecular charge. To reduce the distance between the DNA charge and sensor surface and thus, to enhance the electrostatic coupling between the dsDNA molecules and the LAPS, the negatively charged dsDNA molecules were electrostatically adsorbed onto the gate surface of the LAPS covered with a positively charged weak polyelectrolyte layer of PAH (poly(allylamine hydrochloride)). The surface potential changes in each spot of the LAPS, induced by the layer-by-layer adsorption of a PAH/dsDNA bilayer, were recorded by means of photocurrent-voltage and constant-photocurrent measurements. In addition, the surface morphology of the gate surface before and after consecutive electrostatic adsorption of PAH and dsDNA layers was studied by atomic force microscopy measurements. Moreover, fluorescence microscopy was used to verify the successful adsorption of dsDNA molecules onto the PAH-modified LAPS surface. A high sensor signal of 25 mV was registered after adsorption of 10 nM dsDNA molecules. The lower detection limit is down to 0.1 nM dsDNA. The obtained results demonstrate that the PAH-modified LAPS device provides a convenient and rapid platform for the direct label-free electrical detection of in-solution hybridized dsDNA molecules.
KW  - Layer-by-layer adsorption
KW  - Poly(allylamine hydrochloride)
KW  - Label-free detection
KW  - DNA biosensor
KW  - LAPS
KW  - Field effect
Y1  - 2016
U6  - https://doi.org/10.1016/j.snb.2016.02.004
SN  - 0925-4005
IS  - 229
SP  - 506
EP  - 512
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Schusser, Sebastian
A1  - Bäcker, Matthias
A1  - Krischer, M.
A1  - Wenzel, L.
A1  - Leinhos, Marcel
A1  - Poghossian, Arshak
A1  - Biselli, Manfred
A1  - Wagner, P.
A1  - Schöning, Michael Josef
T1  - Enzymatically catalyzed degradation of biodegradable polymers investigated by means of a semiconductor-based field-effect sensor
JF  - Procedia Engineering
N2  - A semiconductor field-effect device has been used for an enzymatically catalyzed degradation of biopolymers for the first time. This novel technique is capable to monitor the degradation process of multiple samples in situ and in real-time. As model system, the degradation of the biopolymer poly(D, L-lactic acid) has been monitored in the degradation medium containing the enzyme lipase from Rhizomucor miehei. The obtained results demonstrate the potential of capacitive field-effect sensors for degradation studies of biodegradable polymers.
KW  - Field-effect sensor
KW  - enzymatic (bio)degradation
KW  - poly(d, l-lactic acid)
KW  - in-situ monitoring
KW  - impedance spectroscopy
Y1  - 2014
U6  - https://doi.org/10.1016/j.proeng.2014.11.689
SN  - 1877-7058
N1  - EUROSENSORS 2014 ; European Conference on Solid-State Transducers <28, 2014>
VL  - 87
SP  - 1314
EP  - 1317
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Poghossian, Arshak
A1  - Schöning, Michael Josef
T1  - Label-free sensing of biomolecules with field-effect devices for clinical applications
JF  - Electroanalysis
N2  - Among the variety of transducer concepts proposed for label-free detection of biomolecules, the semiconductor field-effect device (FED) is one of the most attractive platforms. As medical techniques continue to progress towards diagnostic and therapies based on biomarkers, the ability of FEDs for a label-free, fast and real-time detection of multiple pathogenic and physiologically relevant molecules with high specificity and sensitivity offers very promising prospects for their application in point-of-care and personalized medicine for an early diagnosis and treatment of diseases. The presented paper reviews recent advances and current trends in research and development of different FEDs for label-free, direct electrical detection of charged biomolecules by their intrinsic molecular charge. The authors are mainly focusing on the detection of the DNA hybridization event, antibody-antigen affinity reaction as well as clinically relevant biomolecules such as cardiac and cancer biomarkers.
Y1  - 2014
U6  - https://doi.org/10.1002/elan.201400073
SN  - 1521-4109 (E-Journal); 1040-0397 (Print)
VL  - 26
IS  - 6
SP  - 1197
EP  - 1213
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - CHAP
A1  - Platen, Johannes
A1  - Poghossian, Arshak
A1  - Schöning, Michael Josef
T1  - Microstructured Nanostructures – nanostructuring by means of conventional photolithography and layer-expansion technique
N2  - A new and simple method for nanostructuring using conventional photolithography and layer expansion or pattern-size reduction technique is presented, which can further be applied for the fabrication of different nanostructures and nano-devices. The method is based on the conversion of a photolithographically patterned metal layer to a metal-oxide mask with improved pattern-size resolution using thermal oxidation. With this technique, the pattern size can be scaled down to several nanometer dimensions. The proposed method is experimentally demonstrated by preparing nanostructures with different configurations and layouts, like circles, rectangles, trapezoids, “fluidic-channel”-, “cantilever”- and meander-type structures.
KW  - Biosensor
KW  - Nanostructuring
KW  - layer expansion
KW  - pattern-size reduction
KW  - self-aligned patterning
Y1  - 2006
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:hbz:a96-opus-1477
ER  - 
TY  - CHAP
A1  - Schöning, Michael Josef
A1  - Wagner, Torsten
A1  - Poghossian, Arshak
A1  - Miyamoto, K.I.
A1  - Werner, C.F.
A1  - Krause, S.
A1  - Yoshinobu, T.
T1  - Light-addressable potentiometric sensors for (bio-)chemical sensing and imaging
T2  - Encyclopedia of Interfacial Chemistry: Surface Science and Electrochemistry. Vol. 7
Y1  - 2018
SN  - 9780128097397
SP  - 295
EP  - 308
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Poghossian, Arshak
A1  - Yoshinobu, Tatsuo
A1  - Simonis, A.
A1  - Ecken, H.
A1  - Lüth, Hans
A1  - Schöning, Michael Josef
T1  - Penicillin detection by means of field-effect based sensors: EnFET, capacitive EIS sensor or LAPS?
JF  - Sensors and Actuators B. 78 (2001), H. 1-3
Y1  - 2001
SN  - 0925-4005
SP  - 237
EP  - 242
ER  - 
TY  - JOUR
A1  - Poghossian, Arshak
A1  - Schöning, Michael Josef
T1  - Chemical and biological field-effect sensors for liquids – a status report
JF  - Handbook of biosensors and biochips / ed. Robert S. Marks ... Bd. 1
Y1  - 2007
SN  - 978-0-470-01905-4
SP  - 395
EP  - 412
PB  - Wiley
CY  - Chichester
ER  - 
TY  - JOUR
A1  - Poghossian, Arshak
A1  - Weil, M.
A1  - Cherstvy, A. G.
A1  - Schöning, Michael Josef
T1  - Electrical monitoring of polyelectrolyte multilayer formation by means of capacitive field-effect devices
JF  - Analytical and bioanalytical chemistry
N2  - The semiconductor field-effect platform represents a powerful tool for detecting the adsorption and binding of charged macromolecules with direct electrical readout. In this work, a capacitive electrolyte–insulator–semiconductor (EIS) field-effect sensor consisting of an Al-p-Si-SiO2 structure has been applied for real-time in situ electrical monitoring of the layer-by-layer formation of polyelectrolyte (PE) multilayers (PEM). The PEMs were deposited directly onto the SiO2 surface without any precursor layer or drying procedures. Anionic poly(sodium 4-styrene sulfonate) and cationic weak polyelectrolyte poly(allylamine hydrochloride) have been chosen as a model system. The effect of the ionic strength of the solution, polyelectrolyte concentration, number and polarity of the PE layers on the characteristics of the PEM-modified EIS sensors have been studied by means of capacitance–voltage and constant-capacitance methods. In addition, the thickness, surface morphology, roughness and wettabilityof the PE mono- and multilayers have been characterised by ellipsometry, atomic force microscopy and water contact-angle methods, respectively. To explain potential oscillations on the gate surface and signal behaviour of the capacitive field-effect EIS sensor modified with a PEM, a simplified electrostatic model that takes into account the reduced electrostatic screening of PE charges by mobile ions within the PEM has been proposed and discussed.
Y1  - 2013
U6  - https://doi.org/10.1007/s00216-013-6951-9
SN  - 1432-1130 ; 1618-2642
VL  - 405
IS  - 20
SP  - 6425
EP  - 6436
PB  - Springer
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Poghossian, Arshak
T1  - Recent advances in biologically sensitive field-effect transistors (BioFETs)
JF  - Analyst. 127 (2002)
Y1  - 2002
SN  - 0003-2654
SP  - 1137
EP  - 1151
ER  - 
TY  - CHAP
A1  - Wu, Chunsheng
A1  - Bronder, Thomas
A1  - Poghossian, Arshak
A1  - Schöning, Michael Josef
T1  - DNA-hybridization detection using light-addressable potentiometric sensor modified with gold layer
T2  - Sensoren und Messsysteme 2014 ; Beiträge der 17. GMA/ITG-Fachtagung vom 3. bis 4. Juni 2014 in Nürnberg. (ITG-Fachbericht ; 250)
Y1  - 2014
SN  - 978-3-8007-3622-5
SP  - 1
EP  - 4
PB  - VDE-Verl.
CY  - Düsseldorf
ER  - 
TY  - CHAP
A1  - Huck, Christina
A1  - Poghossian, Arshak
A1  - Buniatyan, V.
A1  - Schöning, Michael Josef
T1  - Multi-parameter detection for supporting monitoring and control of biogas processes in agriculture
T2  - Sensoren und Messsysteme 2014 ; Beiträge der 17. GMA/ITG-Fachtagung vom 3. bis 4. Juni 2014 in Nürnberg. (ITG-Fachbericht ; 250)
Y1  - 2014
SN  - 978-3-8007-3622-5
SP  - 1
EP  - 5
PB  - VDE-Verl.
CY  - Berlin
ER  - 
TY  - CHAP
A1  - Schöning, Michael Josef
A1  - Poghossian, Arshak
A1  - Glück, Olaf
A1  - Thust, Marion
T1  - Electrochemical methods for the determination of chemical variables in aqueous media
T2  - Measurement, instrumentation, and sensors handbook / ed. by John G. Webster [u.a.] Vol. 2 : Electromagnetic, optical, radiation, chemical, and biomedical measurement
Y1  - 2014
SN  - 978-1-4398-4891-3
SP  - 55-1
EP  - 55-54
PB  - CRC Pr.
CY  - Boca Raton, Fla.
ER  - 
TY  - JOUR
A1  - Molinnus, Denise
A1  - Bäcker, Matthias
A1  - Siegert, Petra
A1  - Willenberg, H.
A1  - Poghossian, Arshak
A1  - Keusgen, M.
A1  - Schöning, Michael Josef
T1  - Detection of Adrenaline Based on Substrate Recycling Amplification
JF  - Procedia Engineering
N2  - An amperometric enzyme biosensor has been applied for the detection of adrenaline. The adrenaline biosensor has been prepared by modification of an oxygen electrode with the enzyme laccase that operates at a broad pH range between pH 3.5 to pH 8. The enzyme molecules were immobilized via cross-linking with glutaraldehyde. The sensitivity of the developed adrenaline biosensor in different pH buffer solutions has been studied.
Y1  - 2015
U6  - https://doi.org/10.1016/j.proeng.2015.08.708
SN  - 1877-7058
N1  - Eurosensors 2015
VL  - 120
SP  - 540
EP  - 543
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Abouzar, Maryam H.
A1  - Poghossian, Arshak
A1  - Cherstvy, Andrey G.
A1  - Pedraza, Angela M.
A1  - Ingebrandt, Sven
A1  - Schöning, Michael Josef
T1  - Label-free electrical detection of DNA by means of field-effect nanoplate capacitors: Experiments and modeling
JF  - Physica Status Solidi (a)
N2  - Label-free electrical detection of consecutive deoxyribonucleic acid (DNA) hybridization/denaturation by means of an array of individually addressable field-effect-based nanoplate silicon-on-insulator (SOI) capacitors modified with gold nanoparticles (Au-NP) is investigated. The proposed device detects charge changes on Au-NP/DNA hybrids induced by the hybridization or denaturation event. DNA hybridization was performed in a high ionic-strength solution to provide a high hybridization efficiency. On the other hand, to reduce the screening of the DNA charge by counter ions and to achieve a high sensitivity, the sensor signal induced by the hybridization and denaturation events was measured in a low ionic-strength solution. High sensor signals of about 120, 90, and 80 mV were registered after the DNA hybridization, denaturation, and re-hybridization events, respectively. Fluorescence microscopy has been applied as reference method to verify the DNA immobilization, hybridization, and denaturation processes. An electrostatic charge-plane model for potential changes at the gate surface of a nanoplate field-effect sensor induced by the DNA hybridization has been developed taking into account both the Debye length and the distance of the DNA charge from the gate surface.
Y1  - 2012
U6  - https://doi.org/10.1002/pssa.201100710
SN  - 1862-6319
VL  - 209
SP  - 925
EP  - 934
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Poghossian, Arshak
A1  - Krämer, Melina
A1  - Abouzar, Maryam H.
A1  - Pita, Marcos
A1  - Katz, Evgeny
A1  - Schöning, Michael Josef
T1  - Interfacing of biocomputing systems with silicon chips: Enzyme logic gates based on field-effect devices
JF  - Procedia Chemistry. 1 (2009), H. 1
Y1  - 2009
SN  - 1876-6196
N1  - Proceedings of the Eurosensors XXIII conference ; Eurosensors 23
SP  - 682
EP  - 685
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - CHAP
A1  - Schöning, Michael Josef
A1  - Poghossian, Arshak
A1  - Glück, Olaf
A1  - Thust, Marion
T1  - Electrochemical composition measurement
T2  - Measurement, instrumentation, and sensors handbook: electromagnetic, optical, radiation, chemical, and biomedical measuremen
Y1  - 2014
SN  - 978-1-4398-4891-3
SP  - 55-1
EP  - 55-54
PB  - CRC Pr.
CY  - Boca Raton, Fa.
ET  - 2nd ed.
ER  - 
TY  - JOUR
A1  - Poghossian, Arshak
A1  - Geissler, Hanno
A1  - Schöning, Michael Josef
T1  - Rapid methods and sensors for milk quality monitoring and spoilage detection
JF  - Biosensors and Bioelectronics
Y1  - 2019
U6  - https://doi.org/10.1016/j.bios.2019.04.040
SN  - 0956-5663
VL  - 140
IS  - Article 111272
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Poghossian, Arshak
A1  - Wagner, Holger
A1  - Schöning, Michael Josef
T1  - Functional testing and characterisation of (bio-)chemical sensors on wafer level
JF  - Procedia Chemistry. 1 (2009), H. 1
Y1  - 2009
SN  - 1876-6196
N1  - Proceedings of the Eurosensors XXIII conference ; Eurosensors 23
SP  - 835
EP  - 838
ER  - 
TY  - JOUR
A1  - Abouzar, Maryam H.
A1  - Poghossian, Arshak
A1  - Siqueira, José R. Jr.
A1  - Oliveira, Osvaldo N. Jr.
A1  - Moritz, Werner
A1  - Schöning, Michael Josef
T1  - Capacitive electrolyte-insulator-semiconductor structures functionalised with a polyelectrolyte/enzyme multilayer: New strategy for enhanced field-effect biosensing
JF  - Physica Status Solidi (A). 207 (2010), H. 4
Y1  - 2010
SN  - 1862-6300
N1  - Special Issue: Engineering of Functional Interfaces EnFI 2009
SP  - 884
EP  - 890
ER  - 
TY  - JOUR
A1  - Poghossian, Arshak
A1  - Ingebrandt, S.
A1  - Offenhäusser, A.
A1  - Schöning, Michael Josef
T1  - Field-effect devices for detecting cellular signals
JF  - Seminars in Cell & Developmental Biology. 20 (2009), H. 1
Y1  - 2009
SN  - 1096-3634
SP  - 41
EP  - 48
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Siqueira, José R. Jr.
A1  - Bäcker, Matthias
A1  - Poghossian, Arshak
A1  - Zucolotto, Valtencir
A1  - Oliveira, Osvaldo N. Jr.
A1  - Schöning, Michael Josef
T1  - Associating biosensing properties with the morphological structure of multilayers containing carbon nanotubes on field-effect devices
JF  - Physica status solidi (a). 207 (2010), H. 4
Y1  - 2010
SN  - 1862-6300
N1  - Special Issue: Engineering of Functional Interfaces EnFI 2009
SP  - 781
EP  - 786
ER  -