TY - CHAP A1 - Duwe, A. A1 - Tippkötter, Nils A1 - Ulber, Roland T1 - Lignocellulose-Biorefinery: Ethanol-Focused T2 - Biorefineries N2 - The development prospects of the world markets for petroleum and other liquid fuels are diverse and partly contradictory. However, comprehensive changes for the energy supply of the future are essential. Notwithstanding the fact that there are still very large deposits of energy resources from a geological point of view, the finite nature of conventional oil reserves is indisputable. To reduce our dependence on oil, the EU, the USA, and other major economic zones rely on energy diversification. For this purpose, alternative materials and technologies are being sought, and is most obvious in the transport sector. The objective is to progressively replace fossil fuels with renewable and more sustainable fuels. In this respect, biofuels have a pre-eminent position in terms of their capability of blending with fossil fuels and being usable in existing cars without substantial modification. Ethanol can be considered as the primary renewable liquid fuel. In this chapter enzymes, micro-organisms, and processes for ethanol production based on renewable resources are described. KW - Bioethanol KW - Biorefinery KW - Lignocellulose feedstook Y1 - 2018 U6 - https://doi.org/10.1007/10_2016_72 N1 - Part of the Advances in Biochemical Engineering/Biotechnology book series (ABE,volume 166) SP - 177 EP - 215 PB - Springer CY - Cham ER - TY - CHAP A1 - Wulfhorst, H. A1 - Duwe, A. A1 - Möhring, S. A1 - Jurca, O. A1 - Tippkötter, Nils T1 - Analysis of pretreated biomass by differential scanning 132 calorimetry and multivariate data analysis T2 - New frontiers of biotech-processes (Himmelfahrtstagung) : 02-04 May 2016, Rhein-Mosel-Halle, Koblenz/Germany Y1 - 2016 SP - 132 PB - DECHEMA CY - Frankfurt am Main ER - TY - PAT A1 - Stadtmüller, Ralf A1 - Tippkötter, Nils A1 - Ulber, Roland T1 - Method for production of single-stranded macronucleotides N2 - The invention relates to a method for production of single-stranded macronucleotides by amplifying and ligating an extended monomeric single-stranded target nucleic acid sequence (targetss) into a repetitive cluster of double-stranded target nucleic acid sequences (targetds), and subsequently cloning the construct into a vector (aptagene vector). The aptagene vector is transformed into host cells for replication of the aptagene and isolated in order to optain single-stranded target sequences (targetss). The invention also relates to single-stranded nucleic acids, produced by a method of the invention. Y1 - 2015 N1 - Patent auch unter EP2774996, EP2774996, US2017145460 und US9944966 veröffentlicht. ER - TY - JOUR A1 - Tippkötter, Nils A1 - Stückmann, Henning A1 - Kroll, Stephen A1 - Winkelmann, Gunda A1 - Noack, Udo A1 - Scheper, Thomas A1 - Ulber, Roland T1 - A semi-quantitative dipstick assay for microcystin JF - Analytical and Bioanalytical Chemistry N2 - An immunochromatographic lateral flow dipstick assay for the fast detection of microcystin-LR was developed. Colloid gold particles with diameters of 40 nm were used as red-colored antibody labels for the visual detection of the antigen. The new dipstick sensor is capable of detecting down to 5 µg·l−1 (ppb; total inversion of the color signal) or 1 ppb (observation of color grading) of microcystin-LR. The course of the labeling reaction was observed via spectrometric wave shifts caused by the change of particle size during the binding of antibodies. Different stabilizing reagents showed that especially bovine serum albumin (BSA) and casein increase the assays sensitivity and the conjugate stability. Performance of the dipsticks was quantified by pattern processing of capture zone CCD images. Storage stability of dipsticks and conjugate suspensions over 115 days under different conditions were monitored. The ready-to-use dipsticks were successfully tested with microcystin-LR-spiked samples of outdoor drinking- and salt water and applied to the tissue of microcystin-fed mussels. Y1 - 2009 U6 - https://doi.org/10.1007/s00216-009-2750-8 SN - 1618-2650 VL - 394 IS - 3 SP - 863 EP - 869 PB - springer CY - Berlin ER - TY - CHAP A1 - Möhring, S. A1 - Wulfhorst, H. A1 - Roth, J. A1 - Tippkötter, Nils T1 - Pretreatment strategies for lignocellulosic biomass T2 - New frontiers of biotech-processes (Himmelfahrtstagung) : 02-04 May 2016, Rhein-Mosel-Halle, Koblenz/Germany Y1 - 2016 SP - 131 PB - DECHEMA CY - Frankfurt am Main ER - TY - CHAP A1 - Roth, J. A1 - Möhring, S. A1 - Tippkötter, Nils T1 - Characterization and evaluation of lignocellulosic biomass 130 hydrolysates for ABE fermentation T2 - New frontiers of biotech-processes (Himmelfahrtstagung) : 02-04 May 2016, Rhein-Mosel-Halle, Koblenz/Germany Y1 - 2016 SP - 130 PB - DECHEMA CY - Frankfurt am Main ER - TY - JOUR A1 - Al-Kaidy, Huschyar A1 - Kuthan, Kai A1 - Hering, Thomas A1 - Tippkötter, Nils T1 - Aqueous droplets used as enzymatic microreactors and their electromagnetic actuation JF - Journal of Visualized Experiments N2 - For the successful implementation of microfluidic reaction systems, such as PCR and electrophoresis, the movement of small liquid volumes is essential. In conventional lab-on-a-chip-platforms, solvents and samples are passed through defined microfluidic channels with complex flow control installations. The droplet actuation platform presented here is a promising alternative. With it, it is possible to move a liquid drop (microreactor) on a planar surface of a reaction platform (lab-in-a-drop). The actuation of microreactors on the hydrophobic surface of the platform is based on the use of magnetic forces acting on the outer shell of the liquid drops which is made of a thin layer of superhydrophobic magnetite particles. The hydrophobic surface of the platform is needed to avoid any contact between the liquid core and the surface to allow a smooth movement of the microreactor. On the platform, one or more microreactors with volumes of 10 µL can be positioned and moved simultaneously. The platform itself consists of a 3 x 3 matrix of electrical double coils which accommodate either neodymium or iron cores. The magnetic field gradients are automatically controlled. By variation of the magnetic field gradients, the microreactors' magnetic hydrophobic shell can be manipulated automatically to move the microreactor or open the shell reversibly. Reactions of substrates and corresponding enzymes can be initiated by merging the microreactors or bringing them into contact with surface immobilized catalysts. Y1 - 2016 U6 - https://doi.org/10.3791/54643 SN - 1940-087X IS - Issue 126 ER - TY - JOUR A1 - Tippkötter, Nils A1 - Roth, Jasmine T1 - Purified Butanol from Lignocellulose – Solvent‐Impregnated Resins for an Integrated Selective Removal JF - Chemie Ingenieur Technik N2 - In traditional microbial biobutanol production, the solvent must be recovered during fermentation process for a sufficient space-time yield. Thermal separation is not feasible due to the boiling point of n-butanol. As an integrated and selective solid-liquid separation alternative, solvent impregnated resins (SIRs) were applied. Two polymeric resins were evaluated and an extractant screening was conducted. Vacuum application with vapor collection in fixed-bed column as bioreactor bypass was successfully implemented as butanol desorption step. In course of further increasing process economics, fermentation with renewable lignocellulosic substrates was conducted using Clostridium acetobutylicum. Utilization of SIR was shown to be a potential strategy for solvent removal from fermentation broth, while application of a bypass column allows for product removal and recovery at once. KW - Biofuel KW - Biorefinery KW - Butanol KW - Clostridium acetobutylicum KW - Lignocellulose Y1 - 2020 U6 - https://doi.org/10.1002/cite.202000200 SN - 1522-2640 N1 - Corresponding author: Nils Tippkötter VL - 92 IS - 11 SP - 1741 EP - 1751 PB - Wiley-VCH CY - Weinheim ER - TY - CHAP A1 - Hering, T. A1 - Ulber, Roland A1 - Tippkötter, Nils T1 - Development of a screening system for antimicrobial surfaces T2 - New frontiers of biotech-processes (Himmelfahrtstagung) : 02-04 May 2016, Rhein-Mosel-Halle, Koblenz/Germany Y1 - 2016 SP - 129 PB - DECHEMA CY - Frankfurt am Main ER - TY - CHAP A1 - Capitain, C. A1 - Hering, T. A1 - Tippkötter, Nils A1 - Ulber, Roland T1 - Enzymatic polymerization of lignin model compounds and solubilized lignin in an aqueous ethanol extract T2 - New frontiers of biotech-processes (Himmelfahrtstagung) : 02-04 May 2016, Rhein-Mosel-Halle, Koblenz/Germany Y1 - 2016 SP - 151 EP - 152 PB - DECHEMA CY - Frankfurt am Main ER - TY - JOUR A1 - Tippkötter, Nils A1 - Al-Kaidy, Huschyar A1 - Wollny, Steffen A1 - Ulber, Roland T1 - Functionalized magnetizable particles for downstream processing in single-use systems JF - Chemie Ingenieur Technik N2 - Biotechnological downstream processing is usually an elaborate procedure, requiring a multitude of unit operations to isolate the target component. Besides the disadvantageous space-time yield, the risks of cross-contaminations and product loss grow fast with the complexity of the isolation procedure. A significant reduction of unit operations can be achieved by application of magnetic particles, especially if these are functionalized with affinity ligands. As magnetic susceptible materials are highly uncommon in biotechnological processes, target binding and selective separation of such particles from fermentation or reactions broths can be done in a single step. Since the magnetizable particles can be produced from iron salts and low priced polymers, a single-use implementation of these systems is highly conceivable. In this article, the principles of magnetizable particles, their synthesis and functionalization are explained. Furthermore, applications in the area of reaction engineering, microfluidics and downstream processing are discussed focusing on established single-use technologies and development potential. Y1 - 2013 U6 - https://doi.org/10.1002/cite.201200130 VL - 85 IS - 1-2: Special Issue: Single-Use Technology SP - 76 EP - 86 PB - Wiley CY - Weinheim ER - TY - CHAP A1 - Engel, M. A1 - Thieringer, J. A1 - Tippkötter, Nils T1 - Microbial electrosynthesis for sustainable biobutanol production T2 - New frontiers of biotech-processes (Himmelfahrtstagung) : 02-04 May 2016, Rhein-Mosel-Halle, Koblenz/Germany Y1 - 2016 SP - 77 EP - 78 PB - DECHEMA CY - Frankfurt am Main ER - TY - JOUR A1 - Thiel, Alexander A1 - Tippkötter, Nils A1 - Suck, Kirstin A1 - Sohling, Ulrich A1 - Ruf, Friedrich A1 - Ulber, Roland T1 - New zeolite adsorbents for downstream processing of polyphenols from renewable resources JF - Engineering in Life Sciences N2 - Commercial materials with polyvinylpolypyrrolidone and polymeric amberlites (XAD7HP, XAD16) are commonly used for the adsorptive downstream processing of polyphenols from renewable resources. In this study, beta-zeolite-based adsorbent systems were examined, and their properties were compared to organic resins. Batch adsorption experiments were conducted with synthetic solutions of major polyphenols. Adsorption isotherms and desorption characteristics of individual adsorbent were determined based on these results. Maximum adsorption capacities were calculated using the Langmuir model. For example, the zeolites had capacities up to 203.2 mg/g for ferulic acid. To extend these results to a complex system, additional experiments were performed on rapeseed meal and wheat seed extracts as representative renewable resources. HPLC analysis showed that with 7.5% w/v, which is regarded as the optimum amount of zeolites, zeolites A and B could bind 100% of the major polyphenols as well as release polyphenols at high yields. Additionally, regeneration experiments were performed with isopropyl alcohol at 99°C to evaluate how zeolites regenerate under mild conditions. The results showed only a negligible loss of adsorption capacity and no loss of desorption capacity. In summary, it was concluded that beta-zeolites were promising adsorbents for developing new processes to isolate polyphenols from renewable resources. Y1 - 2013 U6 - https://doi.org/10.1002/elsc.201200188 VL - 13 IS - 3 SP - 239 EP - 246 PB - Wiley CY - Weinheim ER - TY - JOUR A1 - Teumer, T. A1 - Capitain, C. A1 - Ross-Jones, J. A1 - Tippkötter, Nils A1 - Rädle, M. A1 - Methner, F.-J. T1 - In-line Haze Monitoring Using a Spectrally Resolved Back Scattering Sensor JF - BrewingScience N2 - In the present work an optical sensor in combination with a spectrally resolved detection device for in-line particle-size-monitoring for quality control in beer production is presented. The principle relies on the size and wavelength dependent backscatter of growing particles in fluids. Measured interference structures of backscattered light are compared with calculated theoretical values, based on Mie-Theory, and fitted with a linear least square method to obtain particle size distributions. For this purpose, a broadband light source in combination with a process-CCD-spectrometer (charge ? coupled device spectrometer) and process adapted fiber optics are used. The goal is the development of an easy and flexible measurement device for in-line-monitoring of particle size. The presented device can be directly installed in product fill tubes or vessels, follows CIP- (cleaning in place) and removes the need of sample taking. A proof of concept and preliminary results, measuring protein precipitation, are presented. Y1 - 2018 SN - 1613-2041 VL - 71 IS - 5/6 SP - 49 EP - 55 PB - Fachverlag Hans Carl CY - Nürnberg ER - TY - JOUR A1 - Rösch, C. A1 - Kratz, F. A1 - Hering, T. A1 - Trautmann, S. A1 - Umanskaya, N. A1 - Tippkötter, Nils A1 - Müller-Renno, C.M. A1 - Ulber, Roland A1 - Hannig, M. A1 - Ziegler, C. T1 - Albumin-lysozyme interactions: cooperative adsorption on titanium and enzymatic activity JF - Colloids and Surfaces B: Biointerfaces N2 - The interplay of albumin (BSA) and lysozyme (LYZ) adsorbed simultaneously on titanium was analyzed by gel electrophoresis and BCA assay. It was found that BSA and lysozyme adsorb cooperatively. Additionally, the isoelectric point of the respective protein influences the adsorption. Also, the enzymatic activity of lysozyme and amylase (AMY) in mixtures with BSA was considered with respect to a possible influence of protein-protein interaction on enzyme activity. Indeed, an increase of lysozyme activity in the presence of BSA could be observed. In contrast, BSA does not influence the activity of amylase. Y1 - 2016 U6 - https://doi.org/10.1016/j.colsurfb.2016.09.048 VL - 149 IS - 1 SP - 115 EP - 121 PB - Elsevier CY - Amsterdam ER - TY - CHAP A1 - Wagemann, Kurt A1 - Tippkötter, Nils T1 - Biorefineries: a short introduction T2 - Biorefineries N2 - The terms bioeconomy and biorefineries are used for a variety of processes and developments. This short introduction is intended to provide a delimitation and clarification of the terminology as well as a classification of current biorefinery concepts. The basic process diagrams of the most important biorefinery types are shown. KW - Bioeconomy KW - Biorefinery definitions KW - Introduction KW - Process schemes KW - Renewable resources Y1 - 2018 SN - 978-3-319-97117-9 SN - 978-3-319-97119-3 U6 - https://doi.org/10.1007/10_2017_4 N1 - (Advances in Biochemical Engineering/Biotechnology book series ; Vol. 166) SP - 1 EP - 11 PB - Springer CY - Cham ER - TY - JOUR A1 - Wulfhorst, Helene A1 - Duwe, Anna-Maria A1 - Merseburg, Johannes A1 - Tippkötter, Nils T1 - Compositional analysis of pretreated (beech) wood using differential scanning calorimetry and multivariate data analysis JF - Tetrahedron N2 - The composition of plant biomass varies depending on the feedstock and pre-treatment conditions and influences its processing in biorefineries. In order to ensure optimal process conditions, the quantitative proportion of the main polymeric components of the pre-treated biomass has to be determined. Current standard procedures for biomass compositional analysis are complex, the measurements are afflicted with errors and therefore often not comparable. Hence, new powerful analytical methods are urgently required to characterize biomass. In this contribution, Differential Scanning Calorimetry (DSC) was applied in combination with multivariate data analysis (MVA) to detect the cellulose content of the plant biomass pretreated by Liquid Hot Water (LHW) and Organosolv processes under various conditions. Unlike conventional techniques, the developed analytic method enables the accurate quantification of monosaccharide content of the plant biomass without any previous sample preparation. It is easy to handle and avoids errors in sample preparation. Y1 - 2016 U6 - https://doi.org/10.1016/j.tet.2016.04.029 VL - 72 IS - 46 SP - 7329 EP - 7334 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Tippkötter, Nils A1 - Roikaew, W. A1 - Ulber, Roland T1 - Nitrate removal from whey concentrate with biotechnological regeneration of the waste water JF - European dairy magazine : EDM Y1 - 2008 SN - 0936-6318 IS - 1 SP - 30 EP - 32 ER - TY - CHAP A1 - Tippkötter, Nils A1 - Stückmann, H. A1 - Winkelmann, G. A1 - Noack, U. A1 - Beutel, S. A1 - Scheper, T. A1 - Ulber, Roland T1 - Optimisation of antibody-labelling of gold colloids for their application in an immunchromatographic assay for microcystin-LR T2 - European BioPerspectives : celebrating the 25th DECHEMA annual convention of biotechnologists ; 30 May - 1 June 2007, Cologne, Germany ; book of abstracts ; abstracts, poster programme Y1 - 2007 SP - 126 PB - Dechema CY - Frankfurt am Main ER - TY - JOUR A1 - Tippkötter, Nils A1 - Deterding, A. A1 - Ulber, Roland T1 - Determination of acetic acid in fermentation broth by gas-diffusion technique JF - Engineering in Life Sciences N2 - Due to the interfering effects of acetic acid in many fermentation processes, a gas-diffusion technique was developed for the online determination of acetic acid. The measurements were accomplished with a flow diffusion analysis (FDA) unit from the TRACE Analytics GmbH, Braunschweig, Germany. The diffusion analysis is based on the UV-absorbance of acetic acid at 205 nm. The measurement was achieved by the separation of an acceptor and a carrier stream (acidified fermentation broth) using a gas permeable polytetrafluoroethylene (PTFE) membrane, whereby broth constituents that would otherwise disturb the UV-measurement of acetic acid, are held back efficiently. Merely, the fermentation by-products, e.g. formic acid, is capable of diffusing through the membrane. While formic acid can disturb the measurement, carbon dioxide does not absorb at 205 nm. The method operates with time-dependent sample enrichment. During the analysis, a small volume of the acceptor stream is stopped for a defined time interval in the acceptor chamber. During this period, the gaseous acetic acid diffuses through the membrane and is enriched in the acceptor chamber. Subsequently after the enrichment, the acceptor stream flows through a UV-detector. The intensity of the signal is proportional to the acetic acid concentration. Online measurements in bioreactors via a sterile filtration probe have been accomplished. A linear calibration in the range of 0.5–5.0 g/L acetic acid with a relative standard deviation of <5 % was obtained. A sampling rate of 8 samples per hour was possible. The system was applied for the determination of acetic acid in E. coli fermentation broth. The instrument is easy to clean, very user-friendly and does not require any toxic or expensive reagents. Y1 - 2008 U6 - https://doi.org/10.1002/elsc.200820227 VL - 8 IS - 1, Special Issue: Technical Systems for the Use in Life Sciences SP - 62 EP - 67 ER -