TY  - JOUR
A1  - Balakrishnan, Karthikeyan
A1  - Andrei-Selmer, Luminita-Cornelia
A1  - Selmer, Thorsten
A1  - Bacher, Michael
A1  - Dodel, Richard
T1  - Comparison of Intravenous Immunoglobulins for Naturally Occurring Autoantibodies against Amyloid-β
JF  - Journal of Alzheimer's Disease
Y1  - 2010
SN  - 1387-2877
VL  - 20
IS  - 1
SP  - 135
EP  - 143
ER  - 
TY  - CHAP
A1  - Groebel, Simone
A1  - Werner, Frederik
A1  - Jörres, Niklas
A1  - Jansen, F.
A1  - Kasper, Katharina
A1  - Schiffels, Johannes
A1  - Sprenger, B.
A1  - Baumann, Marcus
A1  - Schöning, Michael Josef
A1  - Selmer, Thorsten
T1  - Entwicklung einer Sensor-Überwachung für Biogasanlagen auf Basis von Prozessdaten einer Parallelanlage
T2  - 10. Dresdner Sensor-Symposium : Dresden, 5. - 7. Dezember 2011 ; miniaturisierte analytische Verfahren, Hochtemperatur-Sensoren, Sensoren für Bioprozess- und Verfahrenstechnik, Sensoren für die Medizin, Chemische Verfahrenstechnik, Lebensmittelanalytik, innovative Sensorlösungen, Sensoren für die Wasserqualität, Selbstüberwachung / Gerald Gerlach ... (Hg.)
N2  - Beim Ausbau nachhaltiger, regenerativer Energieversorgung hat die Umwandlung von organischer Biomasse in Biogas ein großes Potential. Der zugrundeliegende, komplexe biologische Prozess wird noch immer unzureichend verstanden und bedarf systematischer Untersuchungen der Prozessparameter, um einen hohen Ertrag bei guter Gasqualität zu ermöglichen. Die Fragestellungen zur Entschlüsselung des Prozesses sind sowohl verfahrenstechnischer als auch mikrobiologischer Natur. Aus mikrobiologischer Sicht ist die Kenntnis der tatsächlich beteiligten prozesstragenden Mikroorganismen von erheblicher Bedeutung, aus verfahrenstechnischer Sicht die Kenntnis der physikalischen und chemischen Faktoren, welche die mikrobiologischen Prozesse und kontrollieren. Im Zusammenspiel aller dieser Parameter wird die Biogasbildung befördert oder behindert, bis zum Abbruch des Prozesses.
Eine mögliche Kontrollmethode ist die Messung der metabolischen Aktivität prozesstragender Organismen.
Diese soll, beruhend auf fundierten Prozessdaten, gewonnen durch eine Parallelanlage, mit einem lichtadressierbaren potentiometrischen Sensor-System (LAPS) realisiert werden. Dieser Sensor ist in der Lage, pH-Wert-änderungen zu detektieren, die durch den Stoffwechsel der auf dem Chip immobilisierten Organismen hervorgerufen werden, um eine Online-Überwachung von Biogasanlagen zu ermöglichen.
Y1  - 2011
SN  - 978-3-942710-53-4
U6  - http://dx.doi.org/10.5162/10dss2011/4.3
N1  - Dresdner Beiträge zur Sensorik 43 ;
Dresdner Sensor-Symposium ; 10 ; 2011 ; Dresden ; Forschungsgesellschaft für Messtechnik, Sensorik und Medizintechnik ; Zehntes Dresdner Sensor-Symposium ; Dresdner Sensor-Symposium ; (10 ; 2011.12.05-07 ; Dresden)
SP  - 81
EP  - 84
PB  - TUDpress
CY  - Dresden
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Jennemann, Richard
A1  - Bauer, Bernhard L.
A1  - Bertalanffy, Helmut
T1  - Novel glycoinositolphosphosphingolipids, basidiolipids, from Agaricus / Jennemann, Richard ; Bauer, Bernhard, L. ; Bertalanffy, Helmut ; Geyer, Rudolf ; Gschwind, Ruth, M. ; Selmer, Thorsten ; Wiegandt, Herbert
JF  - European Journal of Biochemistry. 259 (1999), H. 1-2
Y1  - 1999
SN  - 0014-2956
SP  - 331
EP  - 338
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Hallmann, Armin
A1  - Schmidt, Bernhard
A1  - Sumper, Manfred
T1  - The Evolutionary Conservation of a Novel Protein Modification, the Conversion of Cysteine to Serinesemialdehyde in Arylsulfatase from Volvox carteri / Selmer, Thorsten ; Hallmann, Armin ; Schmidt, Bernhard ; Sumper, Manfred ; Figura, Kurt von
JF  - European Journal of Biochemistry. 238 (1996), H. 2
Y1  - 1996
SN  - 0014-2956
SP  - 341
EP  - 345
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Scott, Richard
A1  - Näser, Ulrike
A1  - Friedrich, Peter
T1  - Stereochemistry of hydrogen removal from the 'unactivated' C-3 position of 4-hydroxybutyryl-CoA catalysed by 4-hydroxybutyryl-CoA dehydratase / Scott, R. ; Näser, U. ; Friedrich, P. ; Selmer, T. ; Buckel, W. ; Golding, BT.
JF  - Chemical Communications : ChemCom (2004)
Y1  - 2004
SN  - 1364-548X
SP  - 1210
EP  - 1211
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Thamer, Wiebke
A1  - Cirpus, Irina
A1  - Hans, Marcus
T1  - A two [4Fe-4S]-cluster-containing ferredoxin as an alternative electron donor for 2-hydroxyglutaryl-CoA dehydratase from Acidaminococcus fermentans / Thamer, Wiebke ; Cirpus, Irina ; Hans, Marcus ; Pierik, Antonio, J. ; Selmer, Thorsten ; Bill, Eckhard ;
JF  - Archives of Microbiology. 179 (2003), H. 3
Y1  - 2003
SN  - 1432-072X
SP  - 197
EP  - 204
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Netz, Daili Jacqueline Aguilar
A1  - Pohl, Regula
A1  - Beck-Sickinger, Annette G.
T1  - Biochemical characterisation and genetic analysis of aureocin A53, a new, atypical bacteriocin from Staphylococcus aureus / Netz, Daili Jacqueline Aguilar ; Pohl, Regula ; Beck-Sickinger, Annette G. ; Selmer, Thorsten ; Pierik, Antonio J. ;  Carmo de Frei
JF  - Journal of Molecular Biology. 319 (2002), H. 3
Y1  - 2002
SN  - 0022-2836
SP  - 745
EP  - 756
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Schmidt, Bernhard
A1  - Ingendoh, Arnd
A1  - Figura, Kurt von
T1  - A novel amino acid modification in sulfatases that is defective in multiple sulfatase deficiency / Schmidt, Bernhard ; Selmer, Thorsten ;  Ingendoh, Arnd ;  Figurat, Kurt von
JF  - Cell. 82 (1995), H. 2
Y1  - 1995
SN  - 0092-8674
SP  - 271
EP  - 278
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Kahnt, Jörg
A1  - Goubeaud, Marcel
A1  - Shima, Seigo
T1  - The biosynthesis of methylated amino acids in the active site region of methyl-coenzyme M reductase / Selmer, Thorsten ; Kahnt, Jörg ; Goubeaud, Marcel ; Shima, Seigo ; Grabarse, Wolfgang ; Ermler, Ulrich ; Thauer, Rudolf K.
JF  - Journal of Biological Chemistry. 275 (2000), H. 6
Y1  - 2000
SN  - 1083-351X
SP  - 3775
EP  - 3760
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Brüser, Thomas
A1  - Dahl, Christiane
T1  - ADP Sulfurylase" from Thiobacillus denitrificans Is an Adenylylsulfate:Phosphate Adenylyltransferase and Belongs to a New Family of Nucleotidyltransferases / Brüser, Thomas ; Selmer, Thorsten ; Dahl, Christiane
JF  - Journal of Biological Chemistry. 275 (2000), H. 3
Y1  - 2000
SN  - 1083-351X
SP  - 1691
EP  - 1690
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Buckel, Wolfgang
T1  - Oxygen Exchange between Acetate and the Catalytic Glutamate Residue in Glutaconate CoA-transferase from Acidaminococcus fermentans. IMPLICATIONS FOR THE MECHANISM OF CoA-ESTER HYDROLYSIS
JF  - Journal of Biological Chemistry. 274 (1999), H. 30
Y1  - 1999
SN  - 1083-351X
SP  - 20772
EP  - 20778
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Recksiek, Michael
A1  - Dierks, Thomas
A1  - Schmidt, Bernhard
T1  - Sulfatases, Trapping of the Sulfated Enzyme Intermediate by Substituting the Active Site Formylglycine / Recksiek, Michael ; Selmer, Thorsten ; Dierks, Thomas ; Schmidt, Bernhard ; Figura, Kurt von
JF  - Journal of Biological Chemistry. 273 (1998), H. 11
Y1  - 1998
SN  - 1083-351X
SP  - 6096
EP  - 6103
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Miech, Claudia
A1  - Dierks, Thomas
A1  - Figura, Kurt von
T1  - Arylsulfatase from Klebsiella pneumoniae Carries a Formylglycine Generated from a Serine / Miech, Claudia ; Dierks, Thomas ; Selmer, Thorsten ; Figura, Kurt von ; Schmidt, Bernd
JF  - Journal of Biological Chemistry. 273 (1998), H. 9
Y1  - 1998
SN  - 1083-351X
SP  - 4835
EP  - 4837
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Sommerlade, Hans-Jörg
A1  - Ingendoh, Arnd
A1  - Gieselmann, Volkmar
T1  - Glycosylation and phosphorylation of arylsulfatase A / Sommerlade, Hans-Jörg. ; Selmer, Thomas. ; Ingendoh, Arnd ; Gieselmann, Volkmar ; Figura, Kurt von ; Neifer, Klaus ; Schmidt, Bernhard
JF  - Journal of Biological Chemistry. 269 (1994), H. 33
Y1  - 1994
SN  - 1083-351X
SP  - 20977
EP  - 20981
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Jennemann, Richard
A1  - Bauer, Bernhard L.
A1  - Bertalanffy, Helmut
T1  - Basidiolipids from Agaricus are novel immune adjuvants / Jennemann, R. ; Bauer, BL. ; Bertalanffy, H. ; Selmer, T. ; Wiegandt, H.
JF  - Immunobiology. 200 (1999), H. 2
Y1  - 1999
SN  - 0171-2985
N1  - PMID: 10416134
SP  - 277
EP  - 289
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Figura, Kurt von
A1  - Schmidt, Bernhard
A1  - Dierks, T.
T1  - A novel protein modification generating an aldehyde group in sulfatases: its role in catalysis and disease / Figura, Kurt von ; Schmidt, Bernhard ; Selmer, Thorsten ; Dierks, Thomas
JF  - Bioessays. 20 (1998), H. 6
Y1  - 1998
SN  - 1521-1878
SP  - 505
EP  - 510
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Lukatela, G.
A1  - Krauss, N.
A1  - Theis, K.
T1  - Crystal structure of human arylsulfatase A: the aldehyde function and the metal ion at the active site suggest a novel mechanism for sulfate ester hydrolysis / Lukatela, G. ; Krauss, N. ; Theis, K. ; Selmer, T. ; Gieselmann, V. ; Figura, K. von ; Saenger,
JF  - Biochemistry. 37 (1998), H. 11
Y1  - 1998
SP  - 3654
EP  - 3664
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Kim, Jihoe
A1  - Darley, Daniel
A1  - Buckel, Wolfgang
T1  - Characterization of (R)-2-hydroxyisocaproate dehydrogenase and a family III coenzyme A transferase involved in reduction of L-leucine to isocaproate by Clostridium difficile / Kim, J. ; Darley, D. ; Selmer, T. ; Buckel, W.
JF  - Applied and Environmental Microbiology. 72 (2006), H. 9
Y1  - 2006
SN  - 0099-2240
N1  - PMID: 16957230
SP  - 6062
EP  - 6069
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Yu, Lihua
A1  - Blaser, Martin
A1  - Andrei, Paula I.
T1  - 4-Hydroxyphenylacetate decarboxylases: properties of a novel subclass of glycyl radical enzyme systems / Yu, L. ; Blaser, M. ; Andrei, PI. ; Pierik, AJ. Selmer, T.
JF  - Biochemistry. 31 (2006), H. 45
Y1  - 2006
N1  - PMID: 16878993
SP  - 9584
EP  - 9592
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Pierik, Antonio J.
A1  - Heider, Johann
T1  - New glycyl radical enzymes catalysing key metabolic steps in anaerobic bacteria
JF  - Biological Chemistry. 386 (2005), H. 10
Y1  - 2005
SN  - 1431-6730
SP  - 981
EP  - 988
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Achebach, Stephanie
A1  - Unden, Gottfried
T1  - Properties and significance of apoFNR as a second form of air-inactivated [4Fe-4S]·FNR of Escherichia coli / Achebach, S. ; Selmer, T. ; Unden, G.
JF  - The FEBS Journal. 272 (2005), H. 16
Y1  - 2005
SN  - 1742-464X
SP  - 4260
EP  - 4269
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Hermann, Gloria
A1  - Jessen, Holly
A1  - Gokarn, Ravi R.
T1  - Two beta-alanyl-CoA:ammonia lyases in Clostridium propionicum / Herrmann , G. ;  Selmer,  T. ; Jessen,  HJ. ; Gokarn,  RR. ; Selifonova,  O. ;  Gort , SJ. ; , Buckel, W.
JF  - The FEBS Journal. 272 (2005), H. 3
Y1  - 2005
SN  - 1742-464X
SP  - 813
EP  - 821
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Andrei, Paula I.
A1  - Pierik, Antonio J.
A1  - Zauner, Stefan
T1  - Subunit composition of the glycyl radical enzyme p-hydroxyphenylacetate decarboxylase. A small subunit, HpdC, is essential for catalytic activity / Andrei, PI. ;  Pierik,  AJ. ; Zauner , S. ; Andrei-Selmer, LC.  ; Selmer, T.
JF  - European Journal of Biochemistry. 271 (2004), H. 11
Y1  - 2004
SN  - 0014-2956
SP  - 2225
EP  - 2230
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Hetzel, Marc
A1  - Brock, Matthias
A1  - Pierik, Antonio J.
T1  - Acryloyl-CoA reductase from Clostridium propionicum. An enzyme complex of propionyl-CoA dehydrogenase and electron-transferring flavoprotein  / Hetzel, Marc ; Brock, Matthias ; Selmer, Thorsten, Pierik, Antonio J. ; Golding, Bernard T. ; Buckel, Wolfgang
JF  - European Journal of Biochemistry. 270 (2003), H. 5
Y1  - 2003
SN  - 0014-2956
SP  - 902
EP  - 910
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Willanzheimer, Angela
A1  - Hetzel, Marc
T1  - Propionate CoA-transferase from Clostridium propionicum. Cloning of the gene and identification of glutamate 324 at the active site
JF  - European Journal of Biochemistry. 269 (2002), H. 1
Y1  - 2002
SN  - 0014-2956
SP  - 372
EP  - 380
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Andrei, Paula I.
T1  - p-Hydroxyphenylacetate decarboxylase from Clostridium difficile. A novel glycyl radical enzyme catalysing the formation of p-cresol
JF  - European Journal of Biochemistry. 268 (2001), H. 5
Y1  - 2001
SN  - 0014-2956
SP  - 1363
EP  - 1372
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Darley, Dan J.
A1  - Clegg, William
A1  - Harrington, Ross W.
T1  - Stereocontrolled Synthesis of (2R,3S)-2-Methylisocitrate, a Central Intermediate in the Methylcitrate Cycle / Darley, Dan J. ; Selmer, Thorsten ; Clegg, William ; Harrington, Ross W. ; Buckel, Wolfgang ; Golding, Bernardt
JF  - Helvetica chimica acta. 86 (2003), H. 12
Y1  - 2003
SN  - 1522-2675
SP  - 3991
EP  - 3999
ER  - 
TY  - BOOK
A1  - Selmer, Thorsten
T1  - Nachweis einer neuartigen posttranslationalen Modifikation in Sulfatasen und ihr Fehlen in Enzymen aus Patienten mit multipler Sulfatase-Defizienz
Y1  - 1996
N1  - Zugl.: Göttingen, Univ., Diss., 1996
PB  - Cuvillier
CY  - Göttingen
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Pinkenburg, Olaf
T1  - Method of cloning at least one nucleic acid molecule of interest using type IIS restriction endonucleases, and corresponding cloning vectors, kits and system using type IIS restriction endonucleases / Selmer, Thorsten ; Pinkenburg, Olaf
Y1  - 2008
N1  - Patent No. WO 2008095927  	A1  	Aug 14, 2008
ER  - 
TY  - JOUR
A1  - Werner, Frederik
A1  - Groebel, Simone
A1  - Schuhmacher, K.
A1  - Spelthahn, Heiko
A1  - Wagner, Torsten
A1  - Selmer, Thorsten
A1  - Baumann, Marcus
A1  - Schöning, Michael Josef
T1  - Bestimmung der metabolischen Aktivität von Mikroorganismen während des Biogasbildungsprozesses
JF  - 9. Dresdner Sensor-Symposium : Dresden, 07.-09. Dezember 2009 / Gerlach, Gerald ; Hauptmann, Peter [Hrsg.]
Y1  - 2009
SN  - 978-3-941298-44-6
N1  - Dresdner Sensor-Symposium ; (9, 2009, Dresden)
SP  - 201
EP  - 204
PB  - TUDpress
CY  - Dresden
ER  - 
TY  - JOUR
A1  - Werner, Frederik
A1  - Krumbe, Christoph
A1  - Schumacher, Katharina
A1  - Groebel, Simone
A1  - Spelthahn, Heiko
A1  - Stellberg, Michael
A1  - Wagner, Torsten
A1  - Yoshinobu, Tatsuo
A1  - Selmer, Thorsten
A1  - Keusgen, Michael
A1  - Baumann, Marcus
A1  - Schöning, Michael Josef
T1  - Determination of the extracellular acidification of Escherichia coli by a light-addressable potentiometric sensor
JF  - Physica status solidi (a) : applications and material science. 208 (2011), H. 6
Y1  - 2011
SN  - 1862-6319
SP  - 1340
EP  - 1344
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Werner, Frederik
A1  - Groebel, Simone
A1  - Wagner, Torsten
A1  - Yoshinobu, Tatsuo
A1  - Selmer, Thorsten
A1  - Baumann, Marcus
A1  - Schöning, Michael Josef
T1  - Überwachung der metabolischen Aktivität von Mikroorganismen zur Kontrolle des biologischen Prozesses im Biogasfermenter
JF  - Biogas 2011 : Energieträger der Zukunft ; 6. Fachtagung, Fachtagung Braunschweig, 08. und 09. Juni 2011 / VDI Energie und Umwelt
Y1  - 2011
SN  - 978-3-18-092121-1
N1  - Gesellschaft Energie und Umwelt ; Fachtagung Biogas ; (6 : ; 2011.06.08-09 : ; Braunschweig) ; 	VDI-Berichte ; 2121
SP  - 285
EP  - 286
PB  - VDI-Verl.
CY  - Düsseldorf
ER  - 
TY  - JOUR
A1  - Martins, Berta M.
A1  - Blaser, Martin
A1  - Feliks, Mikolaj
A1  - Ullmann, Matthias G.
A1  - Buckel, Wolfgang
A1  - Selmer, Thorsten
T1  - Structural basis for a Kolbe-type decarboxylation catalyzed by a glycyl radical enzyme
JF  - Journal of the American Chemical Society
Y1  - 2011
N1  - Just accepted manuscript
SP  - 1
EP  - 33
PB  - ACS Publications
CY  - Washington, DC
ER  - 
TY  - JOUR
A1  - Schiffels, Johannes
A1  - Baumann, Marcus
A1  - Selmer, Thorsten
T1  - Facile analysis of short-chain fatty acids as 4-nitrophenyl esters in complex anaerobic fermentation samples by high performance liquid chromatography
JF  - Journal of Chromatography A. 1218 (2011), H. 34
Y1  - 2011
SN  - 0021-9673
SP  - 5848
EP  - 5851
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Werner, Frederik
A1  - Groebel, Simone
A1  - Krumbe, Christoph
A1  - Wagner, Torsten
A1  - Selmer, Thorsten
A1  - Yoshinobu, Tatsuo
A1  - Baumann, Marcus
A1  - Schöning, Michael Josef
T1  - Nutrient concentration-sensitive microorganism-based biosensor
JF  - Physica Status Solidi (a)
Y1  - 2012
U6  - http://dx.doi.org/10.1002/pssa.201100801
SN  - 1862-6319
VL  - 209
IS  - 5
SP  - 900
EP  - 904
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Schiffels, Johannes
A1  - Pinkenburg, Olaf
A1  - Schelden, Maximilian
A1  - Aboulnaga, El-Hussiny A. A.
A1  - Baumann, Marcus
A1  - Selmer, Thorsten
T1  - An innovative cloning platform enables large-scale production and maturation of an oxygen-tolerant [NiFe]-hydrogenase from cupriavidus necator in Escherichia coli
JF  - PLOS one. 2013
Y1  - 2013
U6  - http://dx.doi.org/10.1371/journal.pone.0068812
SN  - 1932-6203
PB  - Public Library of Science
CY  - San Francisco, California
ER  - 
TY  - JOUR
A1  - Abulnaga, El-Hussiny
A1  - Pinkenburg, Olaf
A1  - Schiffels, Johannes
A1  - E-Refai, Ahmed
A1  - Buckel, Wolfgang
A1  - Selmer, Thorsten
T1  - Effect of an Oxygen-Tolerant Bifurcating Butyryl Coenzyme A Dehydrogenase/Electron-Transferring Flavoprotein Complex from Clostridium difficile on Butyrate Production in Escherichia coli
JF  - Journal of bacteriology
Y1  - 2013
SN  - 1098-5530 [E-Journal]
SN  - 0021-9193 [Print]
VL  - 195
IS  - 16
SP  - 3704
EP  - 3713
ER  - 
TY  - JOUR
A1  - Aboulnaga, E. H.
A1  - Pinkenburg, O.
A1  - Schiffels, Johannes
A1  - El-Refai, A.
A1  - Buckel, W.
A1  - Selmer, Thorsten
T1  - Butyrate production in Escherichia coli: Exploitation of an oxygen tolerant bifurcating butyryl-CoA dehydrogenase/electron transferring flavoprotein complex from Clostridium difficile
JF  - Journal of bacteriology. June 14, 2013
Y1  - 2013
SN  - 1098-5530 (E-Journal) ; 0021-9193 (Print)
SP  - Epub ahead of print
ER  - 
TY  - JOUR
A1  - Heine, A.
A1  - Herrmann, G.
A1  - Selmer, Thorsten
A1  - Terwesten, F.
A1  - Buckel, W.
A1  - Reuter, K.
T1  - High resolution crystal structure of clostridium propionicum β-Alanyl-CoA:Ammonia Lyase, a new member of the "Hot Dog Fold" protein superfamily
JF  - Proteins
N2  - Clostridium propionicum is the only organism known to ferment β-alanine, a constituent of coenzyme A (CoA) and the phosphopantetheinyl prosthetic group of holo-acyl carrier protein. The first step in the fermentation is a CoA-transfer to β-alanine. Subsequently, the resulting β-alanyl-CoA is deaminated by the enzyme β-alanyl-CoA:ammonia lyase (Acl) to reversibly form ammonia and acrylyl-CoA. We have determined the crystal structure of Acl in its apo-form at a resolution of 0.97 Å as well as in complex with CoA at a resolution of 1.59 Å. The structures reveal that the enyzme belongs to a superfamily of proteins exhibiting a so called “hot dog fold” which is characterized by a five-stranded antiparallel β-sheet with a long α-helix packed against it. The functional unit of all “hot dog fold” proteins is a homodimer containing two equivalent substrate binding sites which are established by the dimer interface. In the case of Acl, three functional dimers combine to a homohexamer strongly resembling the homohexamer formed by YciA-like acyl-CoA thioesterases. Here, we propose an enzymatic mechanism based on the crystal structure of the Acl·CoA complex and molecular docking. Proteins 2014; 82:2041–2053. © 2014 Wiley Periodicals, Inc.
Y1  - 2014
U6  - http://dx.doi.org/10.1002/prot.24557
SN  - 1097-0134 (E-Journal); 0887-3585 (Print)
VL  - 82
IS  - 9
SP  - 2041
EP  - 2053
PB  - Wiley-Liss
CY  - New York
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Biselli, Manfred
A1  - Selmer, Thorsten
A1  - Öhlschläger, Peter
A1  - Baumann, Marcus
A1  - Förster, Arnold
A1  - Poghossian, Arshak
T1  - Forschung „zwischen“ den Disziplinen: das Institut für Nano- und Biotechnologien
JF  - Analytik news : das Online-Labormagazin für Labor und Analytik
N2  - "Biologie trifft Mikroelektronik", das Motto des Instituts für Nano- und Biotechnologien (INB) an der FH Aachen, unterstreicht die zunehmende Bedeutung interdisziplinär geprägter Forschungsaktivitäten. Der thematische Zusammenschluss grundständiger Disziplinen, wie die Physik, Elektrotechnik, Chemie, Biologie sowie die Materialwissenschaften, lässt neue Forschungsgebiete entstehen, ein herausragendes Beispiel hierfür ist die Nanotechnologie: Hier werden neue Werkstoffe und Materialien entwickelt, einzelne Nanopartikel oder Moleküle und deren Wechselwirkung untersucht oder Schichtstrukturen im Nanometerbereich aufgebaut, die neue und vorher nicht bekannte Eigenschaften hervorbringen.

Vor diesem Hintergrund bündelt das im Jahre 2006 gegründete INB die an der FH Aachen vorhandenen Kompetenzen von derzeit insgesamt sieben Laboratorien auf den Gebieten der Halbleitertechnik und Nanoelektronik, Nanostrukturen und DNA-Sensorik, der Chemo- und Biosensorik, der Enzymtechnologie, der Mikrobiologie und Pflanzenbiotechnologie, der Zellkulturtechnik, sowie der Roten Biotechnologie synergetisch. In der Nano- und Biotechnologie steckt außergewöhnliches Potenzial! Nicht zuletzt deshalb stellen sich die Forscher der Herausforderung, in diesem Bereich gemeinsam zu forschen und Schnittstellen zu nutzen, um so bei der Gestaltung neuartiger Ideen und Produkte mitzuwirken, die zukünftig unser alltägliches Leben verändern werden.

Im Folgenden werden die verschiedenen Forschungsbereiche kurz zusammenfassend vorgestellt und vorhandene Interaktionen anhand von exemplarisch ausgewählten, aktuellen Forschungsprojekten skizziert.
Y1  - 2012
VL  - Publ. online
PB  - Dr. Beyer Internet-Beratung
CY  - Ober-Ramstadt
ER  - 
TY  - RPRT
A1  - Schöning, Michael Josef
A1  - Selmer, Thorsten
A1  - Baumann, Marcus
T1  - Schlussbericht zum Projekt "Bio-LAPS" : Optimierung des Betriebs eines Biogasfermenters mit Hilfe eines Feldeffekt-Biosensors auf Basis eines lichtadressierbaren potentiometrischen Sensors (LAPS) : Laufzeit: 01.09.2008 bis 31.01.2012 : Förderkennzeichen 07NR264 bzw.22026407
Y1  - 2012
PB  - BMELV
CY  - Berlin
ER  - 
TY  - CHAP
A1  - Kaspar, K.
A1  - Groebel, Simone
A1  - Kuperjans, Isabel
A1  - Dielmann, Klaus-Peter
A1  - Selmer, Thorsten
T1  - Charakterisierung der Biozönose von Biogasfermentern in Abhängigkeit verschiedener Substrate
T2  - Biogas 2013 : 6. Innovationskongress, 23. - 24.05.2013, Osnabrück, Tagungsband
Y1  - 2013
SN  - 978-3-9813776-3-7
SP  - 69
EP  - 74
PB  - Profair Consult+Project
CY  - Hildesheim
ER  - 
TY  - JOUR
A1  - Pilas, Johanna
A1  - Iken, Heiko
A1  - Selmer, Thorsten
A1  - Keusgen, Michael
A1  - Schöning, Michael Josef
T1  - Development of a multi‐parameter sensor chip for the simultaneous detection of organic compounds in biogas processes
JF  - Physica status solidi (a)
N2  - An enzyme-based multi-parameter biosensor is developed for monitoring the concentration of formate, d-lactate, and l-lactate in biological samples. The sensor is based on the specific dehydrogenation by an oxidized β-nicotinamide adenine dinucleotide (NAD+)-dependent dehydrogenase (formate dehydrogenase, d-lactic dehydrogenase, and l-lactic dehydrogenase, respectively) in combination with a diaphorase from Clostridium kluyveri (EC 1.8.1.4). The enzymes are immobilized on a platinum working electrode by cross-linking with glutaraldehyde (GA). The principle of the determination scheme in case of l-lactate is as follows: l-lactic dehydrogenase (l-LDH) converts l-lactate into pyruvate by reaction with NAD+. In the presence of hexacyanoferrate(III), the resulting reduced β-nicotinamide adenine dinucleotide (NADH) is then regenerated enzymatically by diaphorase. The electrochemical detection is based on the current generated by oxidation of hexacyanoferrate(II) at an applied potential of +0.3 V vs. an Ag/AgCl reference electrode. The biosensor will be electrochemically characterized in terms of linear working range and sensitivity. Additionally, the successful practical application of the sensor is demonstrated in an extract from maize silage.
Y1  - 2015
U6  - http://dx.doi.org/10.1002/pssa.201431894
SN  - 1862-6319
VL  - 212
IS  - 6
SP  - 1306
EP  - 1312
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Schiffels, Johannes
A1  - Selmer, Thorsten
T1  - A flexible toolbox to study protein-assisted metalloenzyme assembly in vitro
JF  - Biotechnology and Bioengineering
Y1  - 2015
U6  - http://dx.doi.org/10.1002/bit.25658
SN  - 1097-0290
VL  - 112
IS  - 11
SP  - 2360
EP  - 2372
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Pilas, Johanna
A1  - Mariano, K.
A1  - Keusgen, M.
A1  - Selmer, Thorsten
A1  - Schöning, Michael Josef
T1  - Optimization of an Enzyme-based Multi-parameter Biosensor for Monitoring Biogas Processes
JF  - Procedia Engineering
Y1  - 2015
U6  - http://dx.doi.org/10.1016/j.proeng.2015.08.702
SN  - 1877-7058
N1  - Part of special issue "Eurosensors 2015"
VL  - 120
SP  - 532
EP  - 535
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - CHAP
A1  - Kasper, Katharina
A1  - Schiffels, Johannes
A1  - Krafft, Simone
A1  - Kuperjans, Isabel
A1  - Elbers, Gereon
A1  - Selmer, Thorsten
T1  - Biogas Production on Demand Regulated by Butyric Acid Addition
T2  - IOP Conference Series: Earth and Environmental Science. Bd. 32
Y1  - 2016
U6  - http://dx.doi.org/10.1088/1755-1315/32/1/012009
SN  - 1755-1315
N1  - ICARET 2016, International Conference on Advances in Renewable Energy and Technologies, Putrajaya, MY, Feb 23-25, 2016
VL  - 32
SP  - 012009/1
EP  - 012009/4
ER  - 
TY  - JOUR
A1  - Pinkenburg, Olaf
A1  - Schiffels, Johannes
A1  - Selmer, Thorsten
T1  - Das CoLibry-Konzept – ein Werkzeugkasten für die Synthetische Biologie: Bioproduktion
JF  - BIOspektrum
N2  - Regardless of size or destination, synthetic biology starts with com-parably small information units, which need to be combined and properly arranged in order to achieve a certain goal. This may be the de novo synthesis of individual genes from oligonucleotides, a shuffling of protein domains in order to create novel biocatalysts, the assembly of multiple enzyme encoding genes in metabolic pathway design, or strain development at the production stage. The CoLibry concept has been designed in order to close the gap between recombinant production of individual genes and genome editing.
Y1  - 2016
U6  - http://dx.doi.org/10.1007/s12268-016-0734-8
VL  - 22
IS  - 6
SP  - 593
EP  - 595
PB  - Springer
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Muschallik, Lukas
A1  - Molinnus, Denise
A1  - Bongaerts, Johannes
A1  - Pohl, Martina
A1  - Wagner, Torsten
A1  - Schöning, Michael Josef
A1  - Siegert, Petra
A1  - Selmer, Thorsten
T1  - (R,R)-Butane-2,3-diol Dehydrogenase from Bacillus clausii DSM 8716T: Cloning and Expression of the bdhA-Gene, and Initial Characterization of Enzyme
JF  - Journal of Biotechnology
N2  - The gene encoding a putative (R,R)-butane-2,3-diol dehydrogenase (bdhA) from Bacillus clausii DSM 8716T was isolated, sequenced and expressed in Escherichia coli. The amino acid sequence of the encoded protein is only distantly related to previously studied enzymes (identity 33–43%) and exhibited some uncharted peculiarities. An N-terminally StrepII-tagged enzyme variant was purified and initially characterized. The isolated enzyme catalyzed the (R)-specific oxidation of (R,R)- and meso-butane-2,3-diol to (R)- and (S)-acetoin with specific activities of 12 U/mg and 23 U/mg, respectively. Likewise, racemic acetoin was reduced with a specific activity of up to 115 U/mg yielding a mixture of (R,R)- and meso-butane-2,3-diol, while the enzyme reduced butane-2,3-dione (Vmax 74 U/mg) solely to (R,R)-butane-2,3-diol via (R)-acetoin. For these reactions only activity with the co-substrates NADH/NAD+ was observed. The enzyme accepted a selection of vicinal diketones, α-hydroxy ketones and vicinal diols as alternative substrates. Although the physiological function of the enzyme in B. clausii remains elusive, the data presented herein clearly demonstrates that the encoded enzyme is a genuine (R,R)-butane-2,3-diol dehydrogenase with potential for applications in biocatalysis and sensor development.
Y1  - 2017
U6  - http://dx.doi.org/10.1016/j.jbiotec.2017.07.020
SN  - 0168-1656
VL  - 258
SP  - 41
EP  - 50
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Röhlen, Desiree
A1  - Pilas, Johanna
A1  - Schöning, Michael Josef
A1  - Selmer, Thorsten
T1  - Development of an amperometric biosensor platform for the combined determination of l-Malic, Fumaric, and l-Aspartic acid
JF  - Applied Biochemistry and Biotechnology
N2  - Three amperometric biosensors have been developed for the detection of L-malic acid, fumaric acid, and L -aspartic acid, all based on the combination of a malate-specific dehydrogenase (MDH, EC 1.1.1.37) and diaphorase (DIA, EC 1.8.1.4). The stepwise expansion of the malate platform with the enzymes fumarate hydratase (FH, EC 4.2.1.2) and aspartate ammonia-lyase (ASPA, EC 4.3.1.1) resulted in multi-enzyme reaction cascades and, thus, augmentation of the substrate spectrum of the sensors. Electrochemical measurements were carried out in presence of the cofactor β-nicotinamide adenine dinucleotide (NAD+) and the redox mediator hexacyanoferrate (III) (HCFIII). The amperometric detection is mediated by oxidation of hexacyanoferrate (II) (HCFII) at an applied potential of + 0.3 V vs. Ag/AgCl. For each biosensor, optimum working conditions were defined by adjustment of cofactor concentrations, buffer pH, and immobilization procedure. Under these improved conditions, amperometric responses were linear up to 3.0 mM for L-malate and fumarate, respectively, with a corresponding sensitivity of 0.7 μA mM−1 (L-malate biosensor) and 0.4 μA mM−1 (fumarate biosensor). The L-aspartate detection system displayed a linear range of 1.0–10.0 mM with a sensitivity of 0.09 μA mM−1. The sensor characteristics suggest that the developed platform provides a promising method for the detection and differentiation of the three substrates.
Y1  - 2017
U6  - http://dx.doi.org/10.1007/s12010-017-2578-1
SN  - 1559-0291
VL  - 183
SP  - 566
EP  - 581
PB  - Springer
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Pilas, Johanna
A1  - Yazici, Yasemen
A1  - Selmer, Thorsten
A1  - Keusgen, Michael
A1  - Schöning, Michael Josef
T1  - Optimization of an amperometric biosensor array for simultaneous measurement of ethanol, formate, d- and l-lactate
JF  - Electrochimica Acta
N2  - The immobilization of NAD+-dependent dehydrogenases, in combination with a diaphorase, enables the facile development of multiparametric sensing devices. In this work, an amperometric biosensor array for simultaneous determination of ethanol, formate, d- and l-lactate is presented. Enzyme immobilization on platinum thin-film electrodes was realized by chemical cross-linking with glutaraldehyde. The optimization of the sensor performance was investigated with regard to enzyme loading, glutaraldehyde concentration, pH, cofactor concentration and temperature. Under optimal working conditions (potassium phosphate buffer with pH 7.5, 2.5 mmol L-1 NAD+, 2.0 mmol L-1 ferricyanide, 25 °C and 0.4% glutaraldehyde) the linear working range and sensitivity of the four sensor elements was improved. Simultaneous and cross-talk free measurements of four different metabolic parameters were performed successfully. The reliable analytical performance of the biosensor array was demonstrated by application in a clarified sample of inoculum sludge. Thereby, a promising approach for on-site monitoring of fermentation processes is provided.
KW  - Simultaneous determination
KW  - Enzymatic biosensor
KW  - Diaphorase
KW  - Dehydrogenase
Y1  - 2017
U6  - http://dx.doi.org/10.1016/j.electacta.2017.07.119
SN  - 0013-4686
VL  - 251
SP  - 256
EP  - 262
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Demmer, Julius K.
A1  - Chowdhury, Nilanjan Pal
A1  - Selmer, Thorsten
A1  - Ermler, Ulrich
A1  - Buckel, Wolfgang
T1  - The semiquinone swing in the bifurcating electron transferring flavoprotein/butyryl-CoA dehydrogenase complex from Clostridium difficile
JF  - Nature Communications
Y1  - 2017
U6  - http://dx.doi.org/10.1038/s41467-017-01746-3
SN  - 2041-1723
N1  - Article number 1577
VL  - 8
IS  - 1
SP  - 1
EP  - 10
ER  - 
TY  - JOUR
A1  - Molinnus, Denise
A1  - Muschallik, Lukas
A1  - Gonzalez, Laura Osorio
A1  - Bongaerts, Johannes
A1  - Wagner, Torsten
A1  - Selmer, Thorsten
A1  - Siegert, Petra
A1  - Keusgen, Michael
A1  - Schöning, Michael Josef
T1  - Development and characterization of a field-effect biosensor for the detection of acetoin
JF  - Biosensors and Bioelectronics
N2  - A capacitive electrolyte-insulator-semiconductor (EIS) field-effect biosensor for acetoin detection has been presented for the first time. The EIS sensor consists of a layer structure of Al/p-Si/SiO₂/Ta₂O₅/enzyme acetoin reductase. The enzyme, also referred to as butane-2,3-diol dehydrogenase from B. clausii DSM 8716T, has been recently characterized. The enzyme catalyzes the (R)-specific reduction of racemic acetoin to (R,R)- and meso-butane-2,3-diol, respectively. Two different enzyme immobilization strategies (cross-linking by using glutaraldehyde and adsorption) have been studied. Typical biosensor parameters such as optimal pH working range, sensitivity, hysteresis, linear concentration range and long-term stability have been examined by means of constant-capacitance (ConCap) mode measurements. Furthermore, preliminary experiments have been successfully carried out for the detection of acetoin in diluted white wine samples.
Y1  - 2018
U6  - http://dx.doi.org/10.1016/j.bios.2018.05.023
VL  - 115
SP  - 1
EP  - 6
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Pilas, Johanna
A1  - Yazici, Y.
A1  - Selmer, Thorsten
A1  - Keusgen, M.
A1  - Schöning, Michael Josef
T1  - Application of a portable multi-analyte biosensor for organic acid determination in silage
JF  - Sensors
N2  - Multi-analyte biosensors may offer the opportunity to perform cost-effective and rapid analysis with reduced sample volume, as compared to electrochemical biosensing of each analyte individually. This work describes the development of an enzyme-based biosensor system for multi-parametric determination of four different organic acids. The biosensor array comprises five working electrodes for simultaneous sensing of ethanol, formate, d-lactate, and l-lactate, and an integrated counter electrode. Storage stability of the biosensor was evaluated under different conditions (stored at +4 °C in buffer solution and dry at −21 °C, +4 °C, and room temperature) over a period of 140 days. After repeated and regular application, the individual sensing electrodes exhibited the best stability when stored at −21 °C. Furthermore, measurements in silage samples (maize and sugarcane silage) were conducted with the portable biosensor system. Comparison with a conventional photometric technique demonstrated successful employment for rapid monitoring of complex media.
Y1  - 2018
U6  - http://dx.doi.org/10.3390/s18051470
SN  - 1424-8220
VL  - 18
IS  - 5
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Aboulnaga, E. A.
A1  - Zou, H.
A1  - Selmer, Thorsten
A1  - Xian, M.
T1  - Development of a plasmid-based, tunable, tolC-derived expression system for application in Cupriavidus necator H16
JF  - Journal of Biotechnology
N2  - Cupriavidus necator H16 gains increasing attention in microbial research and biotechnological application due to its diverse metabolic features. Here we present a tightly controlled gene expression system for C. necator including the pBBR1-vector that contains hybrid promoters originating from C. necator native tolC-promoter in combination with a synthetic tetO-operator. The expression of the reporter gene from these plasmids relies on the addition of the exogenous inducer doxycycline (dc). The novel expression system offers a combination of advantageous features as; (i) high and dose-dependent recombinant protein production, (ii) tight control with a high dynamic range (On/Off ratio), which makes it applicable for harmful pathways or for toxic protein production, (iii) comparable cheap inducer (doxycycline, dc), (iv) effective at low inducer concentration, that makes it useful for large scale application, (v) rapid, diffusion controlled induction, and (vi) the inducer does not interfere within the cell metabolism. As applications of the expression system in C. necator H16, the growth ability on glycerol was enhanced by constitutively expressing the E. coli glpk gene-encoding for glycerol kinase. Likewise, we used the system to overcome the expression toxicity of mevalonate pathway in C. necator H16. With this system, the mevalonate-genes were successfully introduced in the host and the recombinant strains could produce about 200 mg/l mevalonate.
Y1  - 2018
U6  - http://dx.doi.org/10.1016/j.jbiotec.2018.03.007
SN  - 0168-1656
VL  - 274
SP  - 15
EP  - 27
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Röhlen, Desiree
A1  - Pilas, Johanna
A1  - Dahmen, Markus
A1  - Keusgen, Michael
A1  - Selmer, Thorsten
A1  - Schöning, Michael Josef
T1  - Toward a Hybrid Biosensor System for Analysis of Organic and Volatile Fatty Acids in Fermentation Processes
JF  - Frontiers in Chemistry
N2  - Monitoring of organic acids (OA) and volatile fatty acids (VFA) is crucial for the control of anaerobic digestion. In case of unstable process conditions, an accumulation of these intermediates occurs. In the present work, two different enzyme-based biosensor arrays are combined and presented for facile electrochemical determination of several process-relevant analytes. Each biosensor utilizes a platinum sensor chip (14 × 14 mm²) with five individual working electrodes. The OA biosensor enables simultaneous measurement of ethanol, formate, d- and l-lactate, based on a bi-enzymatic detection principle. The second VFA biosensor provides an amperometric platform for quantification of acetate and propionate, mediated by oxidation of hydrogen peroxide. The cross-sensitivity of both biosensors toward potential interferents, typically present in fermentation samples, was investigated. The potential for practical application in complex media was successfully demonstrated in spiked sludge samples collected from three different biogas plants. Thereby, the results obtained by both of the biosensors were in good agreement to the applied reference measurements by photometry and gas chromatography, respectively. The proposed hybrid biosensor system was also used for long-term monitoring of a lab-scale biogas reactor (0.01 m³) for a period of 2 months. In combination with typically monitored parameters, such as gas quality, pH and FOS/TAC (volatile organic acids/total anorganic carbonate), the amperometric measurements of OA and VFA concentration could enhance the understanding of ongoing fermentation processes.
Y1  - 2018
U6  - http://dx.doi.org/10.3389/fchem.2018.00284
IS  - 6
PB  - Frontiers
CY  - Lausanne
ER  - 
TY  - JOUR
A1  - Schiffels, Johannes
A1  - Selmer, Thorsten
T1  - Combinatorial assembly of ferredoxin‐linked modules in Escherichia coli yields a testing platform for Rnf‐complexes
JF  - Biotechnology and Bioengineering
Y1  - 2019
U6  - http://dx.doi.org/10.1002/bit.27079
IS  - accepted article
SP  - 1
EP  - 36
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Muschallik, Lukas
A1  - Kipp, Carina Ronja
A1  - Recker, Inga
A1  - Bongaerts, Johannes
A1  - Pohl, Martina
A1  - Gelissen, Melanie
A1  - Schöning, Michael Josef
A1  - Selmer, Thorsten
A1  - Siegert, Petra
T1  - Synthesis of α-hydroxy ketones and vicinal diols with the Bacillus licheniformis DSM 13T butane-2, 3-diol dehydrogenase
JF  - Journal of Biotechnology
N2  - The enantioselective synthesis of α-hydroxy ketones and vicinal diols is an intriguing field because of the broad applicability of these molecules. Although, butandiol dehydrogenases are known to play a key role in the production of 2,3-butandiol, their potential as biocatalysts is still not well studied. Here, we investigate the biocatalytic properties of the meso-butanediol dehydrogenase from Bacillus licheniformis DSM 13T (BlBDH). The encoding gene was cloned with an N-terminal StrepII-tag and recombinantly overexpressed in E. coli. BlBDH is highly active towards several non-physiological diketones and α-hydroxyketones with varying aliphatic chain lengths or even containing phenyl moieties. By adjusting the reaction parameters in biotransformations the formation of either the α-hydroxyketone intermediate or the diol can be controlled.
Y1  - 2020
SN  - 2590-1559
U6  - http://dx.doi.org/10.1016/j.jbiotec.2020.09.016
VL  - 202
IS  - Vol. 324
SP  - 61
EP  - 70
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Huck, Christina
A1  - Schiffels, Johannes
A1  - Herrera, Cony N.
A1  - Schelden, Maximilian
A1  - Selmer, Thorsten
A1  - Poghossian, Arshak
A1  - Baumann, Marcus
A1  - Wagner, Patrick
A1  - Schöning, Michael Josef
T1  - Metabolic responses of Escherichia coli upon glucose pulses captured by a capacitive field-effect sensor
JF  - Physica Status Solidi (A)
N2  - Living cells are complex biological systems transforming metabolites taken up from the surrounding medium. Monitoring the responses of such cells to certain substrate concentrations is a challenging task and offers possibilities to gain insight into the vitality of a community influenced by the growth environment. Cell-based sensors represent a promising platform for monitoring the metabolic activity and thus, the “welfare” of relevant organisms. In the present study, metabolic responses of the model bacterium Escherichia coli in suspension, layered onto a capacitive field-effect structure, were examined to pulses of glucose in the concentration range between 0.05 and 2 mM. It was found that acidification of the surrounding medium takes place immediately after glucose addition and follows Michaelis–Menten kinetic behavior as a function of the glucose concentration. In future, the presented setup can, therefore, be used to study substrate specificities on the enzymatic level and may as well be used to perform investigations of more complex metabolic responses. Conclusions and perspectives highlighting this system are discussed.
Y1  - 2013
U6  - http://dx.doi.org/10.1002/pssa.201200900
SN  - 0031-8965
VL  - 210
IS  - 5
SP  - 926
EP  - 931
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Dantism, Shahriar
A1  - Röhlen, Desiree
A1  - Selmer, Thorsten
A1  - Wagner, Torsten
A1  - Wagner, Patrick
A1  - Schöning, Michael Josef
T1  - Quantitative differential monitoring of the metabolic activity of Corynebacterium glutamicum cultures utilizing a light-addressable potentiometric sensor system
JF  - Biosensors and Bioelectronics
Y1  - 2019
U6  - http://dx.doi.org/10.1016/j.bios.2019.111332
VL  - 139
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Pilas, Johanna
A1  - Selmer, Thorsten
A1  - Keusgen, Michael
A1  - Schöning, Michael Josef
T1  - Screen-printed carbon electrodes modified with graphene oxide for the design of a reagent-free NAD+-dependent biosensor array
JF  - Analytical Chemistry
Y1  - 2019
U6  - http://dx.doi.org/10.1021/acs.analchem.9b04481
VL  - 91
IS  - 23
SP  - 15293
EP  - 15299
PB  - ACS Publications
CY  - Washington
ER  - 
TY  - JOUR
A1  - Muschallik, Lukas
A1  - Molinnus, Denise
A1  - Jablonski, Melanie
A1  - Kipp, Carina Ronja
A1  - Bongaerts, Johannes
A1  - Pohl, Martina
A1  - Wagner, Torsten
A1  - Schöning, Michael Josef
A1  - Selmer, Thorsten
A1  - Siegert, Petra
T1  - Synthesis of α-hydroxy ketones and vicinal (R, R)-diols by Bacillus clausii DSM 8716ᵀ butanediol dehydrogenase
JF  - RSC Advances
N2  - α-hydroxy ketones (HK) and 1,2-diols are important building blocks for fine chemical synthesis. Here, we describe the R-selective 2,3-butanediol dehydrogenase from B. clausii DSM 8716ᵀ (BcBDH) that belongs to the metal-dependent medium chain dehydrogenases/reductases family (MDR) and catalyzes the selective asymmetric reduction of prochiral 1,2-diketones to the corresponding HK and, in some cases, the reduction of the same to the corresponding 1,2-diols. Aliphatic diketones, like 2,3-pentanedione, 2,3-hexanedione, 5-methyl-2,3-hexanedione, 3,4-hexanedione and 2,3-heptanedione are well transformed. In addition, surprisingly alkyl phenyl dicarbonyls, like 2-hydroxy-1-phenylpropan-1-one and phenylglyoxal are accepted, whereas their derivatives with two phenyl groups are not substrates. Supplementation of Mn²⁺ (1 mM) increases BcBDH's activity in biotransformations. Furthermore, the biocatalytic reduction of 5-methyl-2,3-hexanedione to mainly 5-methyl-3-hydroxy-2-hexanone with only small amounts of 5-methyl-2-hydroxy-3-hexanone within an enzyme membrane reactor is demonstrated.
Y1  - 2020
U6  - http://dx.doi.org/10.1039/D0RA02066D
SN  - 2046-2069
VL  - 10
SP  - 12206
EP  - 12216
PB  - Royal Society of Chemistry (RSC)
CY  - Cambridge
ER  - 
TY  - RPRT
A1  - Siegert, Petra
A1  - Bongaerts, Johannes
A1  - Wagner, Torsten
A1  - Schöning, Michael Josef
A1  - Selmer, Thorsten
T1  - Abschlussbericht zum Projekt zur Überwachung biotechnologischer Prozesse mittels Diacetyl-/Acetoin-Biosensor und Evaluierung von Acetoin-Reduktasen zur Verwendung in Biotransformationen
Y1  - 2022
N1  - Laufzeit: 01.01.2016 – 31.12.2019 (verlängert bis 31.12.2020)

Förderkennzeichen: 322-8.03.04.02-FH-Struktur 2016/02

Gefördert durch: Ministerium für Innovation, Wissenschaft und Forschung des Landes Nordrhein-Westfalen
CY  - Aachen
ER  -