TY - CHAP A1 - Feuerriegel, Uwe A1 - Pook, Michael A1 - Wersch, Gregor A1 - Wittenhorst, Simon A1 - Becker, Jürgen A1 - Ecker, Markus A1 - Hoffmann, Ulrich A1 - Kunz, Ulrich T1 - Simulation von Wärmeübertragungsprozessen N2 - Mit freundlicher Genehmigung der Autoren und des Oldenbourg Industrieverlags https://www.oldenbourg-industrieverlag.de/de/9783835633223-33223 erschienen als Beitrag im Tagungsband zur AALE-Tagung 2012. 9. Fachkonferenz 4.-5. Mai 2012, Aachen, Fachhochschule. ISBN 9783835633223 S 5-1 S. 127-135 Es werden Ergebnisse unterschiedlicher Projekte aus dem Bereich der Simulation von Wärmeübertragungsprozessen mit Excel-VBA vorgestellt. - Thermische Behandlung hochviskoser Fruchtzubereitungen, verschiedene Projekte und Kooperationen mit der Zentis GmbH & Co. KG, Aachen (J. Becker, U. Feuerriegel, G. Wersch). - Untersuchung des dynamischen Verhaltens von dampfbeheizten Ethylen-Verdampfern. Projekt mit der TGE Gas Engineering GmbH, Bonn (M. Ecker, U. Feuerriegel, U. Hoffmann, S. Wittenhorst). - Dynamische Simulation des axialen Temperaturverlaufs von elektrisch beheizten Rohrreaktoren. Kooperation mit dem Institut für Chemische Verfahrenstechnik, TU Clausthal (U. Feuerriegel, U. Kunz, M. Pook, S. Wittenhorst). KW - Wärmeübertragung Y1 - 2012 ER - TY - BOOK A1 - Jeromin, Günter Erich T1 - Organische Chemie : ein praxisbezogenes Lehrbuch. - 2. Aufl. Y1 - 2006 SN - 3-8171-1732-9 PB - Deutsch CY - Frankfurt am Main ER - TY - BOOK A1 - Jeromin, Günter Erich A1 - Bertau, Martin T1 - Bioorganikum : ein Praktikum der Biotransformationen. - 1. Aufl. Y1 - 2005 SN - 3-527-31245-5 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Wagner, Torsten A1 - Beging, Stefan A1 - Rotter, L. A1 - Poghossian, Arshak A1 - Biselli, Manfred A1 - Zang, Werner A1 - Schöning, Michael Josef T1 - Online-Messsysteme für die automatisierte Charakterisierung von feldeffektbasierten Biosensoren JF - 8. Dresdner Sensor-Symposium : Sensoren für Umwelt, Klima und Sicherheit, Biosensoren und Biosysteme, Sensoren und Sensorsysteme für die Prozesstechnik, Trends in der Sensortechnik, Materialentwicklung für die Sensorik; 8. Dresdner Sensor-Symposium, 10. - 12. Dezember 2007, Dresden / Gerald Gerlach ... (Hg.) Y1 - 2007 SN - 978-3-940046-45-1 N1 - Dresdner Sensor-Symposium <8, 2007, Dresden> ; Dresdner Beiträge zur Sensorik ; 29 SP - 257 EP - 260 PB - TUDpress, Verl. der Wissenschaften CY - Dresden ER - TY - JOUR A1 - Feuerriegel, Uwe A1 - Ma, J. A1 - Hoffmann, U. T1 - Untersuchungen zur Rußfilterregeneration mit Hilfe von Zündhilfen. Ma, J.; Hoffmann, U.; Feuerriegel, U JF - Erdöl und Kohle – Erdgas, Brennstoff-Chemie. 43 (1990) Y1 - 1990 SN - 0014-0058 N1 - Druckausgabe in Jülich unter 63 ZS 034-1990 vorhanden SP - 150 EP - 157 ER - TY - JOUR A1 - Feuerriegel, Uwe A1 - Klose, W. T1 - Reaktionskinetik der katalytischen Methan-Oxidation und experimentelle Untersuchungen zur Desaktivierung eines Palladium-Trägerkatalysators durch Schwefelwasserstoff. Klose, W. ; Feuerriegel, U. JF - Chemie - Ingenieur - Technik. 65 (1993), H. 10 Y1 - 1993 SN - 0009-286X N1 - engl. Titel: Reaction Kinetics of Catalytic Methane Oxidation and Deactivation of a Palladium Catalyst by Hydrogen Sulfide During Catalytic Oxidation of Methane SP - 1242 EP - 1243 ER - TY - JOUR A1 - Feuerriegel, Uwe A1 - Stahlberg, R. T1 - Thermoselect – Energie- und Rohstoffgewinnung, Verfahrensgrundlagen zur unterbrechungslosen Verwertung von Restabfällen. Stahlberg, R.; Feuerriegel, U. JF - Thermoselect : der neue Weg, Restmüll umweltgerecht zu behandeln ; [Tagungsband zur Fachtagung Thermoselect am 19. Januar 1995 in Rastatt/Baden] / Günther Häßler. 2. durchges. und erw. Aufl. Y1 - 1995 SN - 3-9803665-4-5 N1 - Fachtagung THERMOSELECT ; (1995, Rastatt) ; Fachtagung Thermoselect ; (1995.01.19 : ; Rastatt) SP - 13 EP - 32 PB - Goerner CY - Karlsruhe ER - TY - JOUR A1 - Feuerriegel, Uwe A1 - Stahlberg, R. T1 - Untersuchungen an der Thermoselect-Anlage Fondotoce (Italien), Qualitäten der Produkte und Nachweis der Verteilung der chemischen Elemente in den Stoffströmen. Stahlberg, R.; Feuerriegel, U. JF - Thermoselect : der neue Weg, Restmüll umweltgerecht zu behandeln ; [Tagungsband zur Fachtagung Thermoselect am 19. Januar 1995 in Rastatt/Baden] / Günther Häßler. 2. durchges. und erw. Aufl. Y1 - 1995 SN - 3-9803665-4-5 N1 - Fachtagung THERMOSELECT ; (1995, Rastatt) ; Fachtagung Thermoselect ; (1995.01.19 : ; Rastatt) SP - 33 EP - 54 PB - Goerner CY - Karlsruhe ER - TY - JOUR A1 - Feuerriegel, Uwe A1 - Stahlberg, R. A1 - Weisenburger, P. A1 - Steiger, F. T1 - Massen- und Energiebilanzen unter Einbeziehung der Untersuchungen an der Thermoselect-Anlage Fondotoce (Italien). Stahlberg, R.; Feuerriegel, U.; Weisenburger, P.; Steiger, F. JF - Thermoselect : der neue Weg, Restmüll umweltgerecht zu behandeln ; [Tagungsband zur Fachtagung Thermoselect am 19. Januar 1995 in Rastatt/Baden] / Günther Häßler. 2. durchges. und erw. Aufl. Y1 - 1995 SN - 3-9803665-4-5 N1 - Fachtagung THERMOSELECT ; (1995, Rastatt) ; Fachtagung Thermoselect ; (1995.01.19 : ; Rastatt) SP - 55 EP - 84 PB - Goerner CY - Karlsruhe ER - TY - JOUR A1 - Feuerriegel, Uwe A1 - Stahlberg, R. T1 - Thermoselect – Energie- und Rohstoffgewinnung. Stahlberg, R. ; Feuerriegel, U. JF - Abfallwirtschaft Stoffkreisläufe / Terratec '95, Kongress West-Ost-Transfer Umwelt vom 1. bis 3. März 1995. Leipziger Messe. Hrsg. von Hasso Scheffler ... Y1 - 1995 SN - 3-8154-3513-7 N1 - Terratec <1995, Leipzig> ; Kongress West-Ost-Transfer Umwelt <1995, Leipzig> ; Leipziger Messe SP - 179 EP - 194 PB - Teubner CY - Stuttgart [u.a.] ER - TY - JOUR A1 - Feuerriegel, Uwe T1 - Trennen und Trocknen. FH Aachen verfügt über einen Zentrifugentrockner im Technikumsmaßstab JF - Lebensmitteltechnik. 33 (2001), H. 7-8 Y1 - 2001 SN - 0047-4290 SP - 22 EP - 23 ER - TY - BOOK A1 - Feuerriegel, Uwe T1 - Zur Desaktivierung eines Desaktivierung eines Palladium-Trägerkatalysators durch Schwefel¬wasserstoff bei der katalytischen Methanoxidation Y1 - 1993 N1 - Dissertation PB - Univ.-Gesamthochsch. CY - Kassel ER - TY - JOUR A1 - Feuerriegel, Uwe A1 - Klose, W. A1 - Sloboshanin, S. A1 - Goebel, H. [u.a.] T1 - Deactivation of a palladium-supported alumina catalyst by hydrogen sulfide during the oxidation of methane JF - Langmuir: the ACS journal of surfaces and colloids. 10 (1994), H. 10 Y1 - 1994 SN - 0743-74363 SP - 3567 EP - 3570 ER - TY - JOUR A1 - Feuerriegel, Uwe A1 - Stahlberg, R. T1 - Thermoselect – Energie- und Rohstoffgewinnung: Teil 1. Verfahrensgrundlagen zur unterbrechungslosen Verwertung von Restabfällen. Stahlberg, R. ; Feuerriegel, U. JF - Chemische Technik . 46 (1994), H. 5 Y1 - 1994 SN - 0045-6519 SP - 257 EP - 266 ER - TY - JOUR A1 - Feuerriegel, Uwe A1 - Stahlberg, R. T1 - Thermoselect – Energie- und Rohstoffgewinnung: Teil 2. Untersuchungen an der Thermoselect-Anlage Fondotoce (Italien), Qualitäten der Produkte und Nachweis der Verteilung der chemischen Elemente in den Stoffströmen. Stahlberg, R. ; Feuerriegel, U. JF - Chemische Technik . 47 (1995), H. 1 Y1 - 1995 SN - 0045-6519 SP - 1 EP - 10 ER - TY - JOUR A1 - Feuerriegel, Uwe A1 - Stahlberg, R. A1 - Riegel, J. A1 - Steiger, F. T1 - Thermoselect – Energie- und Rohstoffgewinnung: Teil 3. Massen- und Energiebilanz unter Berücksichtigung der Wirtschaftlichkeit der Synthesegasnutzung. Stahlberg, R.; Riegel, J.; Feuerriegel, U.; Steiger, F. JF - Chemische Technik . 47 (1995), H. 3 Y1 - 1995 SN - 0045-6519 SP - 113 EP - 121 ER - TY - JOUR A1 - Feuerriegel, Uwe A1 - Stahlberg, R. T1 - Das Thermoselect-Verfahren zur Energie- und Rohstoffgewinnung – Konzepte, Verfahren, Kosten JF - Thermische Abfallentsorgung – Konzepte, Kosten, Verfahren. Tagung Veitshöchheim, 27./28.06.1995, VDI-Gesellschaft Energietechnik Y1 - 1995 SN - 0083-5560 N1 - VDI-Bericht 1192 SP - 319 EP - 319 PB - VDI-Verl. CY - Düsseldorf ER - TY - JOUR A1 - Feuerriegel, Uwe A1 - Klose, W. T1 - Untersuchungen zum Mechanismus der Desaktivierung eines Palladium-Trägerkatalysators durch Vergiftung mit Schwefelwasserstoff JF - Chemie - Ingenieur - Technik. 70 (1998), H. 4 Y1 - 1998 SN - 0009-286X N1 - Artikel in Englisch unter Chemical engineering & technology. 21(1998)7. S. 562-564 SP - 449 EP - 452 ER - TY - JOUR A1 - Feuerriegel, Uwe A1 - Klose, W. T1 - Investigations into the Mechanism of the Deactivation of a Palladium-Supported Catalyst by Hydrogen Sulfide Poisoning. Klose, W. ; Feuerriegel, U. JF - Chemical engineering & technology. 21 (1998), H. 7 Y1 - 1998 SN - 0930-7516 N1 - gleicher Artikel in Deutsch In: Chem.Ing.Tech 70(1998) No S. 449-452 u.d.T.: Untersuchungen zum Mechanismus der Desaktivierung eines Palladium-Trägerkatalysators durch Vergiftung mit Schwefelwasserstoff SP - 562 EP - 564 ER - TY - JOUR A1 - Feuerriegel, Uwe T1 - Energetische Verwertung von Kunststoffabfällen JF - Chemie und Technologie makromolekularer Stoffe :, 16. Kolloquium. / Fachhochschule / Fachbereich Chemieingenieurwesen. 20. November 1998 an der FH Aachen, Fachbereich Chemieingenieurwesen Y1 - 1998 N1 - FH-Texte. 69; In Jülich unter der Signatur: 61 ZOW 405-16 SP - 169 EP - 189 PB - Fachhochschule Aachen CY - Aachen ER - TY - JOUR A1 - Feuerriegel, Uwe A1 - Künsch, M. A1 - Stahlberg, R. A1 - Steiger, F. T1 - Massen- und Energiebilanz des Thermoselect-Verfahrens JF - Thermoselect-Verfahren zur Ent- und Vergasung von Abfällen / Franz J. Schweitzer [Hrsg.] Y1 - 1994 SN - 3-924511-47-0 PB - EF-Verl. für Energie- u. Umwelttechnik CY - Berlin ER - TY - JOUR A1 - Feuerriegel, Uwe A1 - Stahlberg, R. T1 - Performance of the Thermoselect Demonstration Plant at Fondotoce, Italy: Product Quality and Distribution of Chemical Elements in the Material Flow. Stahlberg, R. ; Feuerriegel, U. JF - Solid waste management: thermal treatment & waste-to-energy technologies : proceedings of an international specialty conference sponsored by the Air & Waste Management Association, [Washington, DC, April 18 - 21, 1995] / comp. by James D. Kilgroe Y1 - 1996 N1 - International Conference on Solid Waste Management, Thermal Treatment and Waste to Energy ; <1995, Washington, DC> PB - Air & Waste Management Assoc. CY - Pittsburgh, Pa. ER - TY - JOUR A1 - Feuerriegel, Uwe A1 - Hoffmann, Ulrich A1 - Walenzik, C. A1 - Kögel, J. T1 - Für Scale-up´s und Betriebsmuster, Projekt Trocknungstechnik an der FH Aachen JF - CITplus : das Praxismagazin für Verfahrenstechnik und Apparatebau. 4 (2001) Y1 - 2001 SN - 1436-2597 SP - 24 EP - 25 ER - TY - JOUR A1 - Kotter, Michael A1 - Lovera, P. A1 - Riekert, L. T1 - Charakterisierung eines Trägerkatalysators durch effektive Diffusionskoeffizienten, Labyrinthfaktor und mittlere Porenradien JF - Berichte der Bunsen-Gesellschaft für Physikalische Chemie N2 - Der effektive Diffusionskoeffizient Deff von Gasen in einem porösen Medium hängt im Knudsen- bzw. Übergangsgebiet vom effektiven Porenradius 〈r〉 der Struktur ab. 〈r〉 kann aus Deff ermittelt werden, sofern der Labyrinthfaktor χ der Struktur aus der Kinetik des Stoffaustausches in den mit einer flüssigen Phase gefüllten Poren separat bestimmt wird. An einem Trägerkatalysator (CuO auf γ-Al₂O₃) ergab sich auf diese Weise ein für die Diffusion maßgebender effektiver Porenradius, der in den Bereich der Makroporen fällt. Ein Vergleich mit dem aus reaktionskinetischen Daten ermittelten effektiven Diffusionskoeffizienten läßt auf eine ungleichmäßige Verteilung der aktiven Komponente im Kontaktkorn schließen. Y1 - 1976 SN - 0005-9021 U6 - http://dx.doi.org/10.1002/bbpc.19760800112 VL - 80 IS - 1 SP - 61 EP - 66 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Kotter, Michael A1 - Riekert, L. T1 - The influence of impregnation, drying and activation on the activity and distribution of CuO on α-Alumina JF - Preparation of Catalysts II : scientific bases for the preparation of heterogeneous catalysts ; Proceedings of the Second International Symposium, Louvain-la-Neuve, September 4-7, 1978 / Eds.: B. Delmon ... - (Studies in surface science and catalysis ; 3) Y1 - 1979 SN - 0-444-41733-8 N1 - International Symposium on the Scientific Bases for the Preparation of Heterogeneous Catalysts <2, 1978, Louvain-la-Neuve> SP - 51 EP - 63 PB - Elsevier CY - Amsterdam [u.a.] ER - TY - JOUR A1 - Kotter, Michael A1 - Heering, J. A1 - Riekert, L. T1 - Diffusion and catalytic reaction in zeolite ZSM-5 / J. Heering ; M. Kotter ; L. Riekert JF - Chemical engineering science . 37 (1982), H. 4 Y1 - 1982 SN - 0009-2509 SP - 581 EP - 584 ER - TY - JOUR A1 - Kotter, Michael A1 - Häfele, E. A1 - Prinz, D. A1 - Riekert, L. T1 - Versuche zur Konvertierung von Wassergas an Zeolith-Katalysatoren / E. Häfele ; M. Kotter ; D. Prinz ; L. Riekert JF - Chemie - Ingenieur - Technik. 55 (1983), H. 2 Y1 - 1983 SN - 0009-286X N1 - ISSN der E-Ausg.: 1522-2640 SP - 148 EP - 149 ER - TY - JOUR A1 - Kotter, Michael T1 - Herstellung von Tränkkatalysatoren als verfahrenstechnische Aufgabe JF - Chemie - Ingenieur - Technik. 55 (1983), H. 3 Y1 - 1983 SN - 0009-286X N1 - ISSN der E-Ausg.: 1522-2640 SP - 179 EP - 185 ER - TY - JOUR A1 - Kotter, Michael A1 - Riekert, L. A1 - Weyland, F. T1 - Deposition of ternary oxides as active components by impregnation of porous carriers JF - Preparation of Catalysts III : scientific bases for the preparation of heterogeneous catalysts ; proceedings of the Third International Symposium, Louvain-la-Neuve, September 6-9, 1982 / ed.: G. Poncelet ... - (Studies in surface science and catalysis ; 16) Y1 - 1983 SN - 0-444-42184-X N1 - International Symposium on the Scientific Bases for the Preparation of Heterogeneous Catalysts <3, 1982, Louvain-la-Neuve> SP - 521 EP - 530 PB - Elsevier CY - Amsterdam [u.a.] ER - TY - JOUR A1 - Kotter, Michael A1 - Riekert, L. T1 - Impregnation-type catalysts with nonuniform distribution of the active component JF - Chemical engineering fundamentals. 2 (1983) Y1 - 1983 SN - 0723-0966 SP - 19 EP - 38 ER - TY - JOUR A1 - Kotter, Michael A1 - Riekert, L. A1 - Weyland, F. T1 - Katalytische Abgasreinigung an Nichtedelmetall-Trägerkontakten JF - Verfahrenstechnik : VT ; Informationen für Planung, Bau und Betrieb von Apparaten und Anlagen. 17 (1983) Y1 - 1983 SN - 0049-5948 N1 - 2. ISSN: 0175-5315 , 3. ISSN: 0341-2644 SP - 607 EP - 615 ER - TY - JOUR A1 - Kotter, Michael A1 - Riekert, L. T1 - Beeinflussung der Aktivität und Selektivität von Tränkkatalysatoren durch die topographische Verteilung der Aktivkomponente JF - Verfahrenstechnik : VT ; Informationen für Planung, Bau und Betrieb von Apparaten und Anlagen. 17 (1983) Y1 - 1983 SN - 0049-5948 N1 - 2. ISSN: 0175-5315 , 3. ISSN: 0341-2644 SP - 639 EP - 658 ER - TY - JOUR A1 - Kotter, Michael A1 - Kirch, M. A1 - Riekert, L. T1 - Herstellung von Tränkkatalysatoren mit Nickel als Aktivkomponente auf porösen Trägern / R. Kirch ; M. Kotter ; L. Riekert JF - Chemiker-Zeitung. 108 (1984), H. 4 Y1 - 1984 SN - 0009-2894 N1 - In der Bibliothek vorhanden: 63 ZS 024-1984 (Bereichsbibliothek Jülich) SP - 121 EP - 129 ER - TY - JOUR A1 - Kotter, Michael A1 - Hammon, Ulrich T1 - Herstellung von Formkörpern mit definierter Porenstruktur / Ulrich Hammon ; Michael Kotter JF - Chemie - Ingenieur - Technik. 56 (1984), H. 6 Y1 - 1984 SN - 0009-286X N1 - ISSN der E-Ausg.: 1522-2640 SP - 455 EP - 463 ER - TY - JOUR A1 - Kotter, Michael A1 - Lintz, Hans-Günther A1 - Weyland, Friedrich T1 - Selektive Reduktion von Stickoxiden in Rauchgasen - Stand der Technik und neue Wege JF - Chemie - Ingenieur - Technik. 58 (1986), H. 8 Y1 - 1986 SN - 0009-286X N1 - ISSN der E-Ausg.: 1522-2640 SP - 617 EP - 623 ER - TY - JOUR A1 - Kotter, Michael A1 - Riekert, L. T1 - Tränkkontakte zur partiellen Oxidation von Buten zu Maleinsäureanhydrid JF - Chemie - Ingenieur - Technik. 59 (1987), H. 9 Y1 - 1987 SN - 0009-286X N1 - ISSN der E-Ausg.: 1522-2640 SP - 733 EP - 734 ER - TY - JOUR A1 - Kotter, Michael A1 - Riekert, Lothar A1 - Weyland, Friedrich T1 - The performance of base metal oxides on ceramic carriers as catalysts for air pollution control JF - International chemical engineering. 25 (1985), H. 3 Y1 - 1985 SN - 0020-6318 SP - 418 EP - 427 ER - TY - JOUR A1 - Kotter, Michael A1 - Hammon, U. T1 - Preparation of pellets with well-defined pore structure / U. Hammon ; A. Kotter JF - International chemical engineering. 26 (1986), H. 4 Y1 - 1986 SN - 0020-6318 SP - 563 EP - 573 ER - TY - JOUR A1 - Mang, Thomas A1 - Kolz, J. A1 - Martins, J. A1 - Kremer, K. T1 - Investigation of Elastomer Foams by Single-Sided NMR / Kolz, J. ; Martins, J. ; Kremer, K. ; Blümich, B. ; Mang, Thomas JF - Kurzfassungen : 3. bis 6. Juli 2006, Nürnberg = Summaries / Deutsche Kautschuk Tagung, 3.7.-6.7.2006 Y1 - 2006 N1 - Deutsche Kautschuk-Tagung ; <2006, Nürnberg > SP - 33 PB - DKG, Dt. Kautschuk-Ges. CY - Frankfurt, Main ER - TY - JOUR A1 - Mang, Thomas A1 - Roosen, C. A1 - Ansorge, M. A1 - Leitner, W. T1 - Gaining pH-control in water/carbon dioxide biphasic systems / Abstract No. 1038 / Roosen, Ch. ; Ansorge, M. ; Mang, Thomas ; Leitner, W. ; Greiner, L. JF - Green solvents for processes : Lake Constance, Friedrichshafen, Germany, 8 - 11 October 2006 ; book of abstracts / DECHEMA e.V. Y1 - 2006 N1 - DECHEMA, Gesellschaft für Chemische Technik und Biotechnologie ; [Conference] ; ((Friedrichshafen) : 2006.10.08-11) PB - DECHEMA CY - Frankfurt am Main ER - TY - JOUR A1 - Mang, Thomas A1 - Roosen, Christoph A1 - Ansorge-Schumacher, Marion A1 - Leitner, Walter T1 - Gaining pH-control in water/carbon dioxide biphasic systems / Roosen, Christoph ; Ansorge-Schumacher, Marion ; Mang, Thomas ; Leitner, Walter ; Greiner, Lasse JF - Green Chemistry. 9 (2007) Y1 - 2007 SN - 1463-9262 SP - 455 EP - 458 ER - TY - JOUR A1 - Mang, Thomas A1 - Kolz, J. A1 - Martins, J. A1 - Kremer, K. T1 - Investigation of the Elastomer-Foam Production with Single-Sided NMR / Kolz, J. ; Martins, J. ; Kremer, K. ; Mang, Thomas ; Blümich, B. JF - Kautschuk Gummi Kunststoffe. 60 (2007), H. 4 Y1 - 2007 SN - 0948-3276 SP - 179 EP - 183 ER - TY - JOUR A1 - Kotter, Michael A1 - Lintz, Hans-Günther T1 - Réduction catalytique séléktive des oxydes d'azote : une concéption originale JF - Entropie. 23 (1987), H. 137/138 Y1 - 1987 SN - 0013-9084 SP - 109 EP - 116 ER - TY - JOUR A1 - Kotter, Michael A1 - Li, D. X. A1 - Riekert, Lothar T1 - Partial oxidation of o-xylene to phthalic anhydride in a structured fixed bed containing a sequence of catalysts JF - New developments in selective oxidation : proceedings of an international symposium ; Rimini, Italy, Sept. 18 - 22, 1989 / eds.: G. Centi ... - (Studies in surface science and catalysis ; 55) Y1 - 1990 SN - 0-444-88694-X SP - 267 EP - 274 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Kotter, Michael A1 - Li, D. X. A1 - Riekert, Lothar T1 - Partielle Oxidation von o-Xylol zu Phthalsäureanhydrid in einem strukturierten Festbettreaktor JF - Katalyse : katalytische Abgasreinigung, Zeolithe, homogene Katalyse, selektive Reaktionen, Herstellung und Charakterisierung, Ionenleiter, Elektrokatalyse, SLPC-Katalysatoren, Suspensionsphase ; Vorträge von der DECHEMA-Jahrestagung 1990, 31. Mai und 1. Juni 1990 / hrsg. von H. Kral. - (Deutsche Gesellschaft für Chemisches Apparatewesen, Chemische Technik und Biotechnologie: DECHEMA-Monographien ; 122) Y1 - 1991 SN - 3-527-10216-7 SP - 93 EP - 123 PB - VCH CY - Weinheim ER - TY - JOUR A1 - Mang, Thomas A1 - Haulena, Friedhelm T1 - Ökologisch verträgliche Dichtstoffe gegen drückendes Wasser JF - Bauchemie von der Forschung bis zur Praxis : (Beiträge der 1. Tagung Bauchemie in München) ; 21./22. November 1997 in München / GDCh-Fachgruppe Bauchemie ; [GDCh, Gesellschaft Deutscher Chemiker] Y1 - 1998 SN - 3-924763-69-0 N1 - GDCh-Monographien 11, Tagung Bauchemie <1, 1997, München> SP - 44 PB - GDCh CY - Frankfurt am Main ER - TY - JOUR A1 - Mang, Thomas T1 - Wiederverwertung von Kunststoff-Verbundmaterial JF - Chemie und Technologie makromolekularer Stoffe. 16. Kolloquium: 20. November 1998 an der FH Aachen, Fachbereich Chemieingenieurwesen Y1 - 1998 N1 - FH-Texte 69. Fachhochschule SP - 191 EP - 213 PB - Fachhochschule Aachen CY - Aachen ER - TY - JOUR A1 - Mang, Thomas A1 - Haulena, Friedhelm T1 - Abdichtmaterialien gegen drückendes Wasser und Diesel mit verbesserter Umweltverträglichkeit JF - Internationale Zeitschrift für Bauinstandsetzen und Baudenkmalpflege = International journal for restoration of buildings and monuments. 5 (1999), H. 1 Y1 - 1999 SN - 0947-4498 SP - 57 ER - TY - JOUR A1 - Mang, Thomas T1 - Abdichtmaterialien gegen drückendes Wasser JF - Bauchemie von der Forschung bis zur Praxis : 3. Tagung Bauchemie in Würzburg ; 27./28. September 2001 in Würzburg / GDCh-Fachgruppe Bauchemie ; [Red.: W. Hiller ...] Y1 - 2002 SN - 3-936028-04-4 N1 - Tagung Bauchemie <3, 2001, Würzburg> GdCh-Monographie 24 ; Monographie / GDCh, Gesellschaft Deutscher Chemiker ; 24 SP - 65 PB - GDCh CY - Frankfurt am Main ER - TY - JOUR A1 - Kotter, Michael A1 - Kirch, M. A1 - Riekert, Lothar T1 - Einfluß von Dispersion und Vorgeschichte dispersen Nickels auf dessen katalytische Eigenschaften bei der Hydrierung von Benzol, CO2 und CO / R. Kirch ; M. Kotter ; L. Riekert JF - Berichte der Bunsen-Gesellschaft für Physikalische Chemie. 88 (1984) Y1 - 1984 SN - 0005-9021 SP - 1054 EP - 1057 ER - TY - JOUR A1 - Kotter, Michael A1 - Greger, M. A1 - Ihme, B. T1 - Nonequilibrium phase transitions with hysteresis in solid catalysts : the system copper-oxygen-propene / M. Greger ; B. Ihme ; M. Kotter ... JF - Berichte der Bunsen-Gesellschaft für Physikalische Chemie. 88 (1984) Y1 - 1984 SN - 0005-9021 SP - 427 EP - 433 ER - TY - JOUR A1 - Kotter, Michael A1 - Hoffmann, U. T1 - Simulation eines geregelten Rührkessels mit LABVIEW / U. Hoffmann ; M. Kotter JF - Virtuelle Instrumente in der Praxis : Messtechnik, Automatisierung ; Begleitband zum Kongress VIP 2002 / Rahman Jamal ... (Hrsg.). - (Praxiswissen Elektronik-Industrie : Messtechnik - Automatisierung) Y1 - 2002 SN - 3-7785-2873-4 N1 - VIP <2002, München> SP - 65 PB - Hüthig CY - Heidelberg [u.a.] ER - TY - JOUR A1 - Kotter, Michael A1 - Riekert, Lothar A1 - Simoncic, V. T1 - Pristup sintezi industrijskih katalizatora JF - Kemija u industriji : casopis kemicara i tehnologa Jugoslavije. 35 (1986) Y1 - 1986 SN - 0022-9830 N1 - Hrsg.: Croatian Society of Chemical Engineers SP - 79 EP - 86 ER - TY - BOOK A1 - Kotter, Michael A1 - Lintz, Hans-Günther T1 - Optimierung der Katalysatorzusammensetzung im Fall der selektiven Reduktion von Stickoxiden durch Ammoniak bei instationärer Reaktionsführung in einem weiten Temperaturbereich. - (Forschungsbericht KfK-PEF ; 33) Y1 - 1987 N1 - ISSN der Serie: 0931-2749 PB - Kernforschungszentrum CY - Karlsruhe ER - TY - BOOK A1 - Kotter, Michael A1 - Lintz, Hans-Günther A1 - Turek, Thomas T1 - Entwicklung eines SCR-Katalysators für instationäre Reaktionsführung. - (Forschungsbericht KfK-PEF ; 79) Y1 - 1991 N1 - ISSN der Serie: 0931-2749 ; Zsfassungen in dt. und engl. Sprache PB - Kernforschungszentrum CY - Karlsruhe ER - TY - JOUR A1 - Mang, Thomas A1 - Kricheldorf, Hans R. T1 - 13-C-NMR sequence analysis. 21. Stereoselectivity of oligopeptide syntheses / Kricheldorf, Hans R. ; Mang, Thomas JF - Die makromolekulare Chemie. 183 (1982), H. 9 Y1 - 1982 SN - 1022-1352 SP - 2093 EP - 2111 ER - TY - JOUR A1 - Mang, Thomas A1 - Kricheldorf, Hans R. T1 - 13-C-NMR sequence analysis. 22. Stereoselectivity of the polymerization of D,L-leucine and D,L-valine N-thiocarboxy anhydrides / Kricheldorf, Hans R. ; Mang, Thomas JF - Die makromolekulare Chemie. 183 (1982), H. 9 Y1 - 1982 SN - 1022-1352 SP - 2113 EP - 2129 ER - TY - JOUR A1 - Mang, Thomas A1 - Kricheldorf, Hans R. T1 - Stereospecificity of peptide synthesis by means of phosphorus derivatives: a model of peptide synthesis in molecular evolution / Kricheldorf, Hans R. ; Mang, Thomas JF - International Journal of Biological Macromolecules. 5 (1983), H. 5 Y1 - 1983 SN - 0141-8130 SP - 258 EP - 266 ER - TY - JOUR A1 - Mang, Thomas A1 - Kricheldorf, Hans R. A1 - Jonté, J. Michael T1 - Polylactones. 1. Copolymerizations of glycolide and &beta-caprolactone / Kricheldorf, Hans R. ; Mang, Thomas ; Jonté, J. Michael JF - Macromolecules. 17 (1984), H. 10 Y1 - 1984 SN - 0024-9297 SP - 2173 EP - 2181 ER - TY - JOUR A1 - Mang, Thomas A1 - Kricheldorf, Hans R. A1 - Jonté, J. Michael T1 - Polylactones, 2. Copolymerization of glycolide with β-propiolactone, χ-butyrolactone or δ-valerolactone / Kricheldorf, Hans R. ; Mang, Thomas ; Jonté, J. Michael JF - Die makromolekulare Chemie. 186 (1985), H. 5 Y1 - 1985 SN - 1022-1352 SP - 955 EP - 976 ER - TY - JOUR A1 - Mang, Thomas A1 - Kricheldorf, Hans R. A1 - Au, M. T1 - Models of molecular evolution. 2. Stereospecificity of dipeptide syntheses by means of cyanamides and carbodiimides / Kricheldorf, Hans R. ; Au, M. ; Mang, Thomas JF - International journal of peptide and protein research. 26 (1985), H. 2 Y1 - 1985 SN - 0300-9769 SP - 149 EP - 157 ER - TY - JOUR A1 - Mang, Thomas T1 - Recycling von Getränkekartons aus Verbundmaterial JF - Kunststoffrecycling in Sachsen ; 1. ; Dresden, 26. - 27. Februar 1992 Y1 - 1992 SP - 34 EP - 43 PB - Techn. Univ. CY - Dresden ER - TY - JOUR A1 - Kotter, Michael A1 - Hammon, Ulrich A1 - Riekert, Lothar T1 - Gewinnung niedriger Olefine aus Methanol bei partiellem Umsatz mit Rückführung / U. Hammon ; M. Kotter ; L. Riekert JF - Chemie - Ingenieur - Technik. 61 (1989), H. 2 Y1 - 1989 SN - 0009-286X N1 - ISSN der E-Ausg.: 1522-2640 SP - 151 EP - 152 ER - TY - JOUR A1 - Kotter, Michael A1 - Riekert, Lothar A1 - Turek, Thomas T1 - Entfernung von Ammoniak aus Abwässern durch Strippung und Sorption an Zeolithen : [Synopse 1724] JF - Chemie - Ingenieur - Technik. 61 (1989), H. 1 Y1 - 1989 SN - 0009-286X N1 - ISSN der E-Ausg.: 1522-2640; Das vollständige Manuskript dieser Arbeit umfasst 45 Seiten. Es ist [lt. Verl.] als Fotokopie oder Mikrofiche MS 1724/88 erhältlich. SP - 74 EP - 75 ER - TY - JOUR A1 - Kotter, Michael A1 - Becker, Dieter A1 - Riekert, Lothar T1 - Zur Selektivität der partiellen Oxidation von Propen an Schalenkatalysatoren / Dieter Becker ; Michael Kotter ; Lothar Riekert JF - Chemie - Ingenieur - Technik. 61 (1989), H. 9 Y1 - 1989 SN - 0009-286X N1 - ISSN der E-Ausg.: 1522-2640 SP - 748 EP - 749 ER - TY - JOUR A1 - Kotter, Michael A1 - Xing Li, Dong T1 - Partielle Oxidation von o-Xylol zu Phthalsäreanhydrid an V/Ti-Schalenkatalysatoren : [Synopsis 1898] JF - Chemie - Ingenieur - Technik. 62 (1990), H. 11 Y1 - 1990 SN - 0009-286X N1 - ISSN der E-Ausg.: 1522-2640 ; Das vollständige Manuskript dieser Arbeit umfasst 33 Seiten. Es ist [lt. Verl.] als Fotokopie oder Mikrofiche MS 1898/90 erhältlich. SP - 950 EP - 951 ER - TY - JOUR A1 - Kotter, Michael T1 - Katalytische Abluftreinigung mit integriertem regenerativem Wärmetausch JF - Chemie - Ingenieur - Technik. 64 (1992), H. 9 Y1 - 1992 SN - 0009-286X N1 - ISSN der E-Ausg.: 1522-2640 ; GVC-Jahrentreffer ; Beitrag 116 SP - 848 ER - TY - JOUR A1 - Kotter, Michael A1 - Lintz, Hans-Günther A1 - Turek, Thomas T1 - Katalytische Stickoxid-Reduktion in einem rotierenden Wärmeübertrager JF - Chemie - Ingenieur - Technik. 64 (1992), H. 5 Y1 - 1992 SN - 0009-286X N1 - ISSN der E-Ausg.: 1522-2640 SP - 446 EP - 448 ER - TY - JOUR A1 - Kotter, Michael A1 - Lintz, Hans-Günther A1 - Wittstock, Klaus T1 - Untersuchung der Zwischenproduktbildung bei der katalytischen Nachverbrennung Iösemittelbeladener Abluft an Nichtedelmetall-Katalysatoren JF - Chemie - Ingenieur - Technik. 66 (1994), H. 10 Y1 - 1994 SN - 0009-286X N1 - ISSN der E-Ausg.: 1522-2640 SP - 1369 EP - 1372 ER - TY - JOUR A1 - Mang, Thomas A1 - Kricheldorf, Hans R. T1 - 13-C-NMR Sequence Analysis 20. Stereospecificity of the polymerization of D,L-Leu-NCA and D,L-Val-NCA / Kricheldorf, Hans R. ; Mang, Thomas JF - Die makromolekulare Chemie. 182 (1981), H. 11 Y1 - 1981 SN - 1022-1352 SP - 3077 EP - 3098 ER - TY - BOOK A1 - Kotter, Michael A1 - Lintz, Hans-Günther A1 - Turek, Thomas T1 - Auslegung eines periodisch alternierend betriebenen SCR-Reaktors Y1 - 1993 N1 - (Forschungsbericht KfK-PEF ; 99) ; ISSN der Serie: 0931-2749 ; Förderkennzeichen: 90/001/3 ; Zsfassungen in dt. und engl. Sprache PB - Kernforschungszentrum CY - Karlsruhe ER - TY - JOUR A1 - Kotter, Michael A1 - Lintz, Hans-Günther A1 - Turek, Thomas T1 - Selective catalytic reduction of nitrogen oxide by use of the Ljungstroem air heater as reactor : a case study JF - Chemical engineering science . 47 (1992), H. 9-11 Y1 - 1992 SN - 0009-2509 SP - 2763 EP - 2768 ER - TY - JOUR A1 - Kotter, Michael A1 - Hammon, Ulrich A1 - Riekert, Lothar T1 - Formation of ethene and propene from methanol on zeolite ZSM-5 II. Preparation of finished catalysts and operation of a fixed-bed pilot plant / U. Hammon ; M. Kotter ; L. Riekert JF - Applied catalysis. 37 (1988) Y1 - 1988 SN - 0166-9834 N1 - ISSN der E-Ausg.: 0166-9834 SP - 155 EP - 174 ER - TY - JOUR A1 - Kotter, Michael A1 - Lintz, Hans-Günther A1 - Riekert, Lothar A1 - Trimm, D. L. T1 - Structure and selectivity changes in vanadia-titania catalysts used to promote the reduction of nitric oxide with ammonia JF - Applied catalysis. 52 (1989), H. 1 Y1 - 1989 SN - 0166-9834 N1 - ISSN der E-Ausg.: 0166-9834 SP - 225 EP - 235 ER - TY - JOUR A1 - Kotter, Michael A1 - Grazyk, J. A1 - Geißle, W. A1 - Roth, A. T1 - Fließeigenschaften von Keramiken für die Herstellung von Katalysatorträgern / J. Grazyk ; W. Gleißle ; A. Roth ; M. Kotter JF - Erdöl & Kohle, Erdgas, Petrochemie : EKEP. 43 (1990), H. 1 Y1 - 1990 SN - 0014-0058 N1 - 2. ISSN: 0367-0716 ; In der Bibliothek (Jülich) vorhanden unter 63 ZS 034 SP - 27 EP - 30 ER - TY - JOUR A1 - Mang, Thomas T1 - Elastomere zum Abdichten gegen drückendes Wasser JF - Summaries : June 30 to July 3, 2003, Nürnberg = Kurzfassungen Y1 - 2003 N1 - Internationale Kautschuk-Tagung ; <2003 : ; 2003.06.30-07.03 : ; Nürnberg> ; International Rubber Conference<2003, Nürnberg> SP - 121 EP - 123 PB - DKG, Dt. Kautschuk-Ges. CY - Frankfurt, Main ER - TY - JOUR A1 - Mang, Thomas T1 - Elastomere zum Abdichten gegen drückendes Wasser JF - Kautschuk Gummi Kunststoffe. 57 (2004), H. 4 Y1 - 2004 SN - 0022-9520 SP - 172 EP - 178 ER - TY - JOUR A1 - Mang, Thomas T1 - Thermosensitive Magnetic Nanoparticles for Bioanalytical and Therapeutical Applications JF - Biomedizinische Technik = Biomedical Engineering. 49 (2004), H. 2 Y1 - 2004 SN - 0013-5585 SP - 1006 EP - 1007 ER - TY - JOUR A1 - Mang, Thomas T1 - Polymer Magnetic Particles for Isolating Biomolecules, Especially Nucleic Acids JF - Biomedizinische Technik = Biomedical Engineering. 49 (2004), H. 2 Y1 - 2004 SN - 0013-5585 SP - 1008 EP - 1009 ER - TY - JOUR A1 - Mang, Thomas T1 - Magnetische Mikro- und Nanopartikel für Medizin und Biotechnik JF - Wässrige Kunststoffdispersionen und ihre Anwendung : Fachbereich Angewandte Naturwissenschaften und Technik / Fachhochschule Aachen. [Schriftleitung: Thomas Mang ...] Y1 - 2005 N1 - FH-Texte ; 71 ; Makromolekulares Kolloquium FH Aachen SP - 137 EP - 150 PB - Fachhochschule Aachen CY - Aachen ER - TY - JOUR A1 - Mang, Thomas A1 - Haulena, Friedhelm T1 - Monodisperse latex particles with functional groups JF - Modification of polymers : 4th Dresden Polymer Discussion ; September 22 to 25, 1992 at Gaussig / Dresden University of Technology, Department of Macromolecular Chemistry and Textile Chemistry; Institute of Polymer Technology Dresden. [Organizers: Claudia Kummerlöwe ...] Y1 - 1992 N1 - Dresdner Polymer Discussion <4, 1992, Gaußig> SP - 68 EP - 88 PB - Univ. CY - Dresden ER - TY - JOUR A1 - Mang, Thomas T1 - Aluminium-Kunststoff-Verbundmaterial trennen und verwerten JF - Umwelt. 24 (1994), H. 4 Y1 - 1994 SN - 0041-6355 SP - 152 EP - 153 ER - TY - JOUR A1 - Mang, Thomas A1 - Haulena, Friedhelm A1 - Rose, Gerd T1 - Trennung und werkstoffliche Verwertung von Aluminium-Kunststoff-Verbundmaterial JF - Aluminium. 70 (1994), H. 5/6 Y1 - 1994 SN - 0002-6689 SP - 333 EP - 339 ER - TY - JOUR A1 - Mang, Thomas T1 - Wässrige Kunststoffdispersionen für Medizin und Biotechnologie JF - Chemie und Technologie makromolekularer Stoffe. 15. Kolloquium: 25. November 1994 an der FH Aachen, Fachbereich Chemieingenieurwesen Y1 - 1994 N1 - FH-Texte 66. Fachhochschule SP - 155 EP - 183 ER - TY - JOUR A1 - Mang, Thomas A1 - Haulena, Friedhelm T1 - Veranstaltung des 15. Kolloquiums Chemie und Technologie makromolekularer Stoffe: wässrige Kunststoffdispersionen, FH Aachen (1994) JF - Farbe und Lack. 101 (1995), H. 1 Y1 - 1995 SN - 0014-7699 SP - 72 EP - 72 ER - TY - JOUR A1 - Mang, Thomas A1 - Haulena, Friedhelm A1 - Rose, Gerd T1 - Werkstoffliche Wiederverwertung von Arzneimittelblistern JF - Kunststoffrecycling in Sachsen. 4 Y1 - 1995 SP - 119 EP - 137 ER - TY - JOUR A1 - Mang, Thomas A1 - Haulena, Friedhelm A1 - Stuckenbruck, U. T1 - Ökologisch verträgliche Dichtstoffe JF - Umwelt. 25 (1995), H. 10 Y1 - 1995 SN - 0041-6355 SP - 430 EP - 441 ER - TY - JOUR A1 - Mang, Thomas A1 - Haulena, Friedhelm A1 - Mathews, W. A1 - Neuroth, N. T1 - Verwertung gebrauchter Teppichböden / Haulena, Friedhelm ; Mang, Thomas ; Mathews, W. ; Neuroth, N. JF - Umwelt. 26 (1996), H. 7/8 Y1 - 1996 SN - 0041-6355 SP - 50 EP - 53 ER - TY - JOUR A1 - Mang, Thomas A1 - Haulena, Friedhelm A1 - Brill, B. T1 - Entsorgung beflockter Verbundwerkstoffe JF - Flock 97 : 14. Internationales Flockseminar Seeheim bei Darmstadt 16. - 17. Januar 1997. Y1 - 1997 N1 - International Flock Symposium <14, 1997, Seeheim> SP - 10-1 EP - 10-18 PB - Flock-Verl. CY - Büdingen ER - TY - JOUR A1 - Mang, Thomas A1 - Haulena, Friedhelm T1 - Recycling of Polymeric Composites JF - Invited and contributing lectures presented at the 38th Microsymposium on Recycling of Polymers : held in Prague, Czech Republic, July 14 - 17, 1997 / International Union of Pure and Applied Chemistry, Macromolecular Division. Symposium ed. J. Kahovec. Y1 - 1998 N1 - Macromolecular symposia ; 135 ; Microsymposium on Recycling of Polymers <38, 1997, Praha> SP - 147 EP - 156 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Elbers, Gereon A1 - Muratyan, Simone T1 - Problematik verkehrsbezogener Außenluftmessungen von Partikeln (Dieselruß) JF - + Krebserzeugende Stoffe in der Umwelt : Herkunft, Messung, Risiko, Minimierung ; Kolloquium Mannheim, 23. bis 25. April 1991 / Kommission Reinhaltung der Luft im VDI und DIN Y1 - 1991 SN - 3-18-090888-2 N1 - VDI-Berichte ; 888 SP - 143 PB - VDI-Verl. CY - Düsseldorf ER - TY - JOUR A1 - Elbers, Gereon A1 - Buck, Manfred T1 - Soot Concentration in Ambient Air / M. Buck, G. Elbers JF - Erdöl & Kohle, Erdgas, Petrochemie : EKEP. 45 (1992) Y1 - 1992 SN - 0014-0058 SP - 219 ER - TY - JOUR A1 - Elbers, Gereon T1 - Messung von Ruß in der Außenluft - Praktische Erfahrungen und Meßergebnisse aus NRW JF - Aktuelle Aufgaben der Messtechnik in der Luftreinhaltung : Kolloquium Heidelberg, 2. bis 4. Juni 1993 / Kommission Reinhaltung der Luft im VDI und DIN Y1 - 1993 SN - 3-18-091059-3 N1 - VDI-Berichte ; 1059 SP - 337 PB - VDI-Verl. CY - Düsseldorf ER - TY - JOUR A1 - Elbers, Gereon A1 - Pfeffer, H.-U. A1 - Friesel, J. A1 - Beier, R. T1 - Pilotuntersuchungen gemäß dem Verordnungsentwurf nach § 40, Abs. 2 des Bundesimmissionsschutzgesetzes / H.-U. Pfeffer, J. Friesel, G. Elbers, R. Beier, K. Ellermann JF - Drei Jahrzehnte Luftqualitätsüberwachung : Vorträge und Berichte zum Kolloquium "Drei Jahrzehnte Luftqualitätsüberwachung - Bilanz und Perspektiven" am 11. November 1993 in Essen / [Hrsg.: Landesanstalt für Immissionsschutz Nordrhein-Westfalen. Red. Bearb.: Robert Niesenhaus] Y1 - 1993 N1 - LIS-Berichte ; 110 ; SP - 117 CY - Essen ER - TY - JOUR A1 - Elbers, Gereon A1 - Richter, J. T1 - Messung kraftfahrzeugbedingter Rußimmissionen JF - Staub, Reinhaltung der Luft : air quality control. 54 (1994) Y1 - 1994 SN - 0039-0771 SP - 19 ER - TY - JOUR A1 - Elbers, Gereon A1 - Bauer, H. D. A1 - Buck, Manfred A1 - Dahmann, D. T1 - Rußmessungen am Arbeitsplatz und in der Außenluft - Vergleich zweier Meßmethoden / H.D. Bauer, M. Buck, D. Dahmann, G. Elbers, H.-H. Fricke JF - Staub, Reinhaltung der Luft : air quality control. 54 (1994) Y1 - 1994 SN - 0039-0771 SP - 55 ER - TY - JOUR A1 - Elbers, Gereon A1 - Pfeffer, Hans-Ulrich A1 - Friesel, Jürgen A1 - Beier, Reinhold T1 - Air Pollution Monitoring in Street Canyons / Hans-Ulrich Pfeffer, Jürgen Friesel, Gereon Elbers, Reinhold Beier, JF - Transport and air pollution : proceedings ; 3rd international symposium, Palais des Papes, Avignon, France, 6 - 10 June 1994 = Transports et pollution de l'air / [Third International Symposium on Transport and Air Pollution]. Ed. by Robert Joumard. Organizer: French National Institute for Transport and Safety Research Y1 - 1995 N1 - International Symposium on Transport and Air Pollution <3, 1994, Avignon> PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Elbers, Gereon A1 - Gäb, Martina A1 - Hoffmann, Katharina A1 - Lobe, Melanie T1 - NIR-spectroscopic investigation of foliage of ozone-stressed Fagus sylvatica trees / Gäb, Martina ; Hoffmann, Katharina ; Lobe, Melanie ; Metzger, Rut ; Ooyen, Sven van ; Elbers, Gereon ; Köllner, Barbara JF - Journal of Forest Research. 11 (2006), H. 2 Y1 - 2006 SN - 1341-6979 SP - 69 EP - 75 ER - TY - BOOK A1 - Elbers, Gereon T1 - Entwicklung eines Schnellmessverfahrens zur Charakterisierung der Wirkung von Umweltschadstoffen auf Pflanzen mit der Nahinfrarot-Spektroskopie : Schlussbericht zum Forschungsvorhaben ; Vorhabensbezeichnung: Schnellmessverfahren zur Charakterisierung der Wirkung von Umweltschadstoffen ; Laufzeit des Vorhabens: 01.09.2002 bis 29.02.2004 ; Verlängerung der Laufzeit: 01.03.2004 bis 30.04.04 ; Berichtszeitraum: 01.09.2002 bis 30.04.2004 / Zuwendungsempfänger: FH-Aachen, Abt. Jülich, FB Chemie und Biotechnik. Gereon Elbers Y1 - 2004 N1 - Förderkennzeichen BMBF 1704502 ; weitere elektr. Ausgabe der TIB Hannover : http://edok01.tib.uni-hannover.de/edoks/e01fb05/485188317.pdf PB - Fachhochschule Aachen CY - Jülich ER - TY - JOUR A1 - Lassonczyk, Beate A1 - Grupe, M. A1 - Wiechmann, H. T1 - Schwierigkeiten bei der Ausgliederung von Bodengesellschaften in typischen Landschaften des Münsterlandes / Grupe, M., B. Lassonczyk und H. Wiechmann JF - Mitteilungen der Deutschen Bodenkundlichen Gesellschaft. 38 (1983) Y1 - 1983 SN - 0343-107x SP - 501 EP - 506 ER - TY - JOUR A1 - Prielmeier, Franz A1 - Lang, E. W. A1 - Radkowitsch, H. A1 - Lüdemann, H.-D. T1 - High Pressure NMR Study of the Molecular Dynamics of Liquid methyl fluoride and deutero-methyl fluoride / Lang, E. W. ; Prielmeier, F. X. ; Radkowitsch, H. ; Lüdemann, H. D. JF - Berichte der Bunsen-Gesellschaft für Physikalische Chemie. 91 (1987), H. 10 Y1 - 1987 SN - 0005-9021 SP - 1017 EP - 1025 ER - TY - JOUR A1 - Prielmeier, Franz A1 - Lang, E. W. A1 - Radkowitsch, H. A1 - Lüdemann, H. D. T1 - High Pressure NMR Study of the Molecular Dynamics of Liquid fluoroform and deutero-fluoroform / Lang, E. W. ; Prielmeier, F. X. ; Radkowitsch, H. ; Lüdemann, H. D. JF - Berichte der Bunsen-Gesellschaft für Physikalische Chemie. 91 (1987), H. 10 Y1 - 1987 SN - 0005-9021 SP - 1025 EP - 1033 ER - TY - JOUR A1 - Prielmeier, Franz A1 - Lang, E. W. A1 - Speedy, R. J. A1 - Lüdemann, H.-D. T1 - Diffusion in supercooled water to 300 MPa JF - Physical Review Letters. 59 (1987), H. 10 Y1 - 1987 SN - 0031-9007 SP - 1128 EP - 1131 ER - TY - JOUR A1 - Prielmeier, Franz A1 - Radkowitsch, H. A1 - Lang, E. W. A1 - Lüdemann, H.-D. T1 - Density dependence of the molecular dynamics of fluid CH3F and CF3H studied by NMR / H. Radkowitsch, F. X. Prielmeier, E. W. Lang and H.-D. Lüdemann JF - Physica B+C. 139-140 (1986) Y1 - 1986 SN - 0921-4526 SP - 96 EP - 99 ER - TY - JOUR A1 - Prielmeier, Franz A1 - Woznyj, M. A1 - Lüdemann, H.-D. T1 - Pressure Dependence of the Melting and Self Diffusion in 2,2-Dimethylpropane, 2,2-Dimethylpropionitrile, and 2-Methylpropanol-2 / M. Woznyj, F. X. Prielmeier, H.-D. Lüdemann JF - Zeitschrift für Naturforschung A, Journal of Physical Sciences. 39 (1984) Y1 - 1984 SN - 0932-0784 SP - 800 ER - TY - JOUR A1 - Prielmeier, Franz A1 - Speedy, R. J. A1 - Lüdemann, H.-D. T1 - High Pressure NMR Self-Diffusion Studies on Supercooled Water JF - High Pressure Science and Technology Proceeding XI AIRAPT, Kiew. 1 Y1 - 1987 N1 - AIRAPT Conference / International Association for the Advancement of High Pressure Science and Technology SP - 75 ER - TY - BOOK A1 - Biselli, Manfred T1 - Enzymkatalysierte Racematspaltung von Aminosäuren mit integrierter Rückführung : dargestellt am Beispiel der Umsetzung von D,L-Alanin zu L-Alanin / Manfred Fridolin Biselli Y1 - 1992 N1 - Berichte des Forschungszentrums Jülich ; 2581 PB - Forschungszentrum Jülich, Zentralbibliothek CY - Jülich ER - TY - BOOK A1 - Biselli, Manfred T1 - Enzymkatalysierte Racematspaltung von Aminosäuren mit integrierter Rückführung : dargestellt am Beispiel der Umsetzung von D,L-Alanin zu L-Alanin / vorgelegt von Manfred Fridolin Biselli Y1 - 1992 N1 - Bonn, Univ., Diss., 1991 CY - Bonn ER - TY - JOUR A1 - Biselli, Manfred A1 - Van der Pol, Jens J. A1 - Machnik, Marc A1 - Portela-Klein, Theresa T1 - On-line immunoanalysis of monoclonal antibodies during a continuous culture of hybridoma cells / Jens J. van der Pol, Marc Machnik, Manfred Biselli, Theresa Portela-Klein, Cornelis D. de Gooijer, Johannes Tramper und Christian Wandrey JF - Cytotechnology. 24 (1997), H. 1 Y1 - 1997 SN - 0920-9069 SP - 19 EP - 30 ER - TY - JOUR A1 - Biselli, Manfred A1 - Meissner, Petra A1 - Schröder, Bernd A1 - Herfurth, Cornelia T1 - Development of a fixed bed bioreactor for the expansion of human hematopoietic progenitor cells / Meissner, Petra ; Schröder, Bernd ; Herfurth, Cornelia ; Biselli, Manfred JF - Cytotechnology. 30 (1999), H. 1 Y1 - 1999 SN - 0920-9069 SP - 227 EP - 234 ER - TY - PAT A1 - Stellberg, Michael A1 - Jeromin, Günter Erich T1 - Rührvorrichtung für Magnetrührsystem : Patentschrift DE19807518C2 ; Veröffentlichungstag der Patenterteilung: 31.05.2000 Y1 - 2000 N1 - Volltext über Datenbank DEPATISnet PB - Deutsches Patent- und Markenamt CY - München ER - TY - PAT A1 - Jeromin, Günter Erich T1 - Kohlensäurediester, deren Herstellung und Verwendung als Riechstoffe und Aromastoffe : Offenlegungsschrift DE19802198A1 ; Offenlegungstag: 29.07.1999 Y1 - 1999 N1 - Volltext über Datenbank: http://publikationen.dpma.de/ PB - Deutsches Patent- und Markenamt CY - München ER - TY - PAT A1 - Jeromin, Günter Erich T1 - Verfahren zur Sanierung von belasteten Böden : Patentschrift DE4113440C2 ; Veröffentlichungstag der Patenterteilung: 02.07.1998 Y1 - 1998 N1 - Volltext über Datenbank: http://publikationen.dpma.de/ PB - Deutsches Patent- und Markenamt CY - München ER - TY - PAT A1 - Jeromin, Günter Erich A1 - Albertz, Michael T1 - Verfahren zur Herstellung von optisch aktiven α-Acetoxycarbonsäuren und optisch aktiven α-Hydroxycarbonsäuren mit Hilfe von Lipasen : Offenlegungsschrift DE4037251A1 ; Offenlegungstag 27.05.1992 Y1 - 1992 N1 - Volltext über Datenbank: http://publikationen.dpma.de/ PB - Deutsches Patent- und Markenamt CY - München ER - TY - PAT A1 - Jeromin, Günter Erich A1 - Scheidt, Annette T1 - Verfahren zur Herstellung von optisch aktiven Alkoholen und optisch aktiven Fettsäureestern mit Hilfe von Lipasen und Vinylestern höherer Fettsäuren : Offenlegungsschrift DE4100394A1 ; Offenlegungstag: 16.07.1992 Y1 - 1992 N1 - Volltext über Datenbank: http://publikationen.dpma.de/ PB - Deutsches Patent- und Markenamt CY - München ER - TY - PAT A1 - Jeromin, Günter Erich T1 - Verfahren zur enzymkatalysierten irreversiblen Veresterung von Carbonsäuren in organischem Lösungsmittel : Offenlegungsschrift DE10210854A1 ; Offenlegungstag: 25.09.2003 Y1 - 2003 N1 - Volltext über Datenbank: http://publikationen.dpma.de/ PB - Deutsches Patent- und Markenamt CY - München ER - TY - BOOK A1 - Gilch, Heinz Gerhard A1 - Rath, Walter T1 - Moisture-curing, polyurethane hot-melt compositions = Feuchtigkeitshärtende Polyurethan-Schmelzklebstoff-Zusammensetzungen Y1 - 1995 N1 - EP 0369607 30.03.1995 Veröffentlichungstag im Patentblatt ; Volltext recherchierbar über: PB - Europäisches Patentamt CY - München ER - TY - BOOK A1 - Rath, Walter T1 - Umsetzung [alpha,beta]-ungesaettigter Ketone und ihrer Vorprodukte mit Urotropin Y1 - 1984 N1 - Stuttgart, Univ., Diss., 1984 ER - TY - BOOK A1 - Müller, Bodo A1 - Rath, Walter T1 - Formulierung von Kleb- und Dichtstoffen : das kompetente Lehrbuch für Studium und Praxis Y1 - 2004 SN - 3-87870-791-6 PB - Vincentz Network CY - Hannover ER - TY - JOUR A1 - Prielmeier, Franz A1 - Nagatomo, Yasushi A1 - Wick, Markus A1 - Frahm, Jens T1 - Dynamic monitoring of cerebral metabolites during and after transient global ischemia in rats by quantitative proton NMR spectroscopy in vivo / Yasushi Nagatomo, Markus Wick, Franz Prielmeier, Jens Frahm JF - NMR in Biomedicine. 8 (1995), H. 6 Y1 - 1995 SN - 1099-1492 SP - 265 EP - 270 ER - TY - JOUR A1 - Elbers, Gereon A1 - Lehmann, G. T1 - Electron paramagnetic resonance of Vanadyl ion impurities in crystalline solids: A comment JF - Journal of Physics and Chemistry of Solids. 46 (1985), H. 6 Y1 - 1985 SN - 0022-3697 SP - 761 ER - TY - JOUR A1 - Elbers, Gereon A1 - Lehmann, G. T1 - Electron Paramagnetic Resonance and Optical Absorption Spectra of VO2+ in CsCl Single Crystals JF - Zeitschrift für Naturforschung / Section A, a journal of physical sciences. 40 (1985) Y1 - 1985 SN - 0932-0784 SP - 511 ER - TY - JOUR A1 - Elbers, Gereon A1 - Behner, T. A1 - Remme, S. A1 - Prissok, F. T1 - Interstitial Metal Impurities as a Possible Cause of Enhanced Reactivities / T. Behner, G. Elbers, S. Remme, F. Prissok, P. Stegger, and G. Lehmann JF - Berichte der Bunsen-Gesellschaft : physical chemistry, chemical physics. 90 (1986) Y1 - 1986 SN - 0005-9021 SP - 698 ER - TY - JOUR A1 - Elbers, Gereon A1 - Remme, S. A1 - Lehmann, G. T1 - EPR of Cr3+ in Tris(acetylacetonato)gallium(III) Single Crystals JF - Inorganic Chemistry. 25 (1986) Y1 - 1986 SN - 0020-1669 SP - 896 EP - 897 ER - TY - JOUR A1 - Elbers, Gereon A1 - Remme, S. A1 - Lehmann, G. T1 - EPR and Optical Absorption of Cr3+ in CsCl and CsBr JF - Physica Status Solidi (B). 142 (1987), H. 2 Y1 - 1987 SN - 0031-8957 SP - 367 EP - 377 ER - TY - JOUR A1 - Elbers, Gereon A1 - Zang, Thorsten A1 - Buck, Manfred T1 - Ruß-Immissionsmessungen im Einflußbereich des Kraftfahrzeugverkehrs JF - Staub, Reinhaltung der Luft : air quality control. 50 (1990) Y1 - 1990 SN - 0039-0771 SP - 93 ER - TY - JOUR A1 - Elbers, Gereon A1 - Thomzik, Manfred T1 - Das Entstehen geruchsintensiver schwefelhaltiger organischer Verbindungen in Lackierereien und Möglichkeiten zu deren Vermeidung JF - Aus der Tätigkeit der LIS / Landesanstalt für Immissionsschutz des Landes Nordrhein-Westfalen. 1989 (1990) Y1 - 1990 SN - 0931-5497 SP - 35 ER - TY - JOUR A1 - Prielmeier, Franz A1 - Wick, Markus A1 - Nagatomo, Yasushi A1 - Frahm, Jens T1 - Alteration of Intracellular Metabolite Diffusion in Rat Brain In Vivo During Ischemia and Reperfusion / Markus Wick, Yasushi Nagatomo, Franz Prielmeier, Jens Frahm JF - Stroke. 26 (1995), H. 10 Y1 - 1995 SN - 0039-2499 SP - 1930 EP - 1934 ER - TY - JOUR A1 - Prielmeier, Franz A1 - Nagatomo, Yasushi A1 - Frahm, Jens T1 - Cerebral blood oxygenation in rat brain during hypoxic hypoxia. Quantitative MRI of effective transverse relaxation rates JF - Magnetic Resonance in Medicine. 31 (1994), H. 6 Y1 - 1994 SN - 0740-3194 SP - 678 EP - 681 ER - TY - BOOK A1 - Merschenz-Quack, Angelika T1 - Untersuchungen zum Stoffwechsel menschlicher Thrombozyten in der Urämie / Angelika Merschenz ; Darge, Walter [Betreuer] Y1 - 1976 N1 - Aachen, Fachhochschule, Diplomarbeit, 1976 ER - TY - JOUR A1 - Prielmeier, Franz A1 - Merboldt, K. D. A1 - Hanicke, W. A1 - Frahm, J. T1 - Dynamic high-resolution MR imaging of brain deoxygenation during transient anoxia in the anesthetized rat JF - Journal of cerebral blood flow and metabolism. 13 (1993), H. 5 Y1 - 1993 SN - 0271-678X SP - 889 EP - 894 ER - TY - JOUR A1 - Prielmeier, Franz A1 - Hörstermann, D. A1 - Gyngell, M. L. A1 - Merboldt, K.-D. T1 - Localized Proton MRS of Acute and Chronic Gyperglycemia in Rat Brain in vivo / D. Hörstermann, F. Prielmeier , M. L. Gyngell, K.-D. Merboldt, W. Hänicke, J. Frahm JF - Book of Abstracts, SMRM, 11th Annual Meeting Berlin Y1 - 1992 N1 - Society of Magnetic Resonance in Medicine SP - 2740 ER - TY - JOUR A1 - Prielmeier, Franz A1 - Speedy, R. J. A1 - Vardag, T. A1 - Lang, E. W. T1 - Diffusion in simple fluids / R. J. Speedy; F. X. Prielmeier; T. Vardag; E. W. Lang; H.-D. Lüdemann JF - Molecular Physics. 66 (1989), H. 3 Y1 - 1989 SN - 0026-8976 SP - 577 EP - 590 ER - TY - JOUR A1 - Prielmeier, Franz A1 - Lüdemann, H.-D. T1 - Self diffusion in compressed liquid chloromethane, dichloromethane and trichloromethane / F. X. Prielmeier; H.-D. Lüdemann JF - Molecular Physics. 58 (1986), H. 3 Y1 - 1986 SN - 0026-8976 SP - 593 EP - 604 ER - TY - JOUR A1 - Prielmeier, Franz A1 - Lang, E. W. A1 - Lüdemann, H.-D. T1 - Pressure dependence of the self-diffusion in liquid trifluoromethane / F. X. Prielmeier; E. W. Lang; H.-D. Lüdemann JF - Molecular Physics. 52 (1984), H. 5 Y1 - 1984 SN - 0026-8976 SP - 1105 EP - 1113 ER - TY - JOUR A1 - Prielmeier, Franz A1 - Lang, E. W. A1 - Speedy, R. J. A1 - Lüdemann, H.-D. T1 - The pressure Dependence of Self Diffusion in Supercooled Light and Heavy Water / F.X. Prielmeier, E .W. Lang, R. J. Speedy, H.-D. Lüdemann JF - Berichte der Bunsen-Gesellschaft für Physikalische Chemie. 92 (1988) Y1 - 1988 SN - 0005-9021 SP - 1111 ER - TY - JOUR A1 - Prielmeier, Franz A1 - Lang, E. W. T1 - Multinuclear Spin-Lattice Relaxation Time Studies of Supercooled Aqueous LiCl-Solutions / E .W. Lang, F. X. Prielmeier JF - Berichte der Bunsen-Gesellschaft für Physikalische Chemie. 92 (1988) Y1 - 1988 SN - 0005-9021 SP - 717 ER - TY - JOUR A1 - Jeromin, Günter Erich A1 - Weise, Sven T1 - Enzymatische Acetylierung von (±)-1-Phthalimido-2-propanol JF - Liebigs Annalen der Chemie (1990) Y1 - 1990 SN - 0947-3440 N1 - ISSN der E-Ausg.: 1099-0690 SP - 1045 EP - 1046 ER - TY - PAT A1 - Jeromin, Günter Erich T1 - Enzymkatalysierte irreversible Veresterung von Carbonsäuren in organischem Lösungsmittel : Offenlegungsschrift DE102005061737A1 ; Offenlegungstag: 05.07.2007 Y1 - 2007 N1 - Volltext über Datenbank: http://publikationen.dpma.de/ PB - Deutsches Patent- und Markenamt CY - München ER - TY - PAT A1 - Jeromin, Günter Erich A1 - Daußmann, Thomas A1 - Hippchen, Hendrik A1 - Riß, Patrick T1 - Verfahren zur enzymatischen Herstellung von optisch aktiven trans-1,2-Cyclopropandicarbonsäurediestern und optisch aktiven trans-1,2-Cyclopropandicarbonsäuremonoestern : Offenlegungsschrift DE102004059469A1 ; Offenlegungsdatum: 14.06.2006 / Erfinder: Jeromin, Günter E. ; Daußmann, Thomas ; Hippchen, Hendrik ; Riß, Patrick Y1 - 2006 N1 - Volltext über Datenbank: http://publikationen.dpma.de/ PB - Deutsches Patent- und Markenamt CY - München ER - TY - BOOK A1 - Schmich, Peter T1 - Identifizierung und Bestimmung von freien Radikalen bei der Kohlenwasserstoff-Pyrolyse : Experimente und Computersimulationen / [vorgelegt von Peter Schmich] Y1 - 1988 N1 - Heidelberg, Univ., Diss., 1988. CY - Heidelberg ER - TY - BOOK A1 - Jeromin, Günter Erich A1 - Nau, Gundula T1 - Sanierung kontaminierter Böden mittels NT-Plasma Y1 - 2000 N1 - Nebent.: Sanierung von schadstoffbelasteten Böden mit Hilfe von Niedertemperaturplasma PB - Fachhochschule Aachen, Abt. Jülich, FB Chemie und Biotechnik CY - Jülich ER - TY - JOUR A1 - Schmich, Peter A1 - Ederer, Hanns J. A1 - Ebert, Klaus H. T1 - Detection and identification of free radicals in hydrocarbon pyrolysis by an iodine trapping method JF - Industrial & Engineering Chemistry Research. 31 (1992), H. 1 Y1 - 1992 SN - 1520-5045 SP - 29 EP - 37 ER - TY - BOOK A1 - Jeromin, Günter Erich A1 - Mang, Thomas T1 - Immobilisierte Enzyme auf magnetisierbaren Trägern / Offenlegungsschrift: DE102004005198A1 ; Offenlegungstag: 25.08.2005 Y1 - 2005 N1 - Volltext über Datenbank DEPATISnet PB - Deutsches Marken- und Patentamt CY - München ER - TY - PAT A1 - Jeromin, Günter Erich A1 - Mang, Thomas T1 - Neue Technik mit magnetisierbaren Materialien : Offenlegungsschrift DE102004005201A1 ; Offenlegungstag: 11.08.2005 Y1 - 2005 N1 - Volltext über Datenbank DEPATISnet PB - Deutsches Patent- und Markenamt CY - München ER - TY - PAT A1 - Jeromin, Günter Erich A1 - Mang, Thomas T1 - Funktionalisierte magnetisierbare Partikel : Offenlegungsschrift DE102004005199A1 ; Offenlegungstag: 18.08.2005 Y1 - 2005 N1 - Volltext über Datenbank DEPATISnet PB - Deutsches Patent- und Markenamt CY - München ER - TY - PAT A1 - Jeromin, Günter Erich T1 - Organische Radikale, deren Herstellung und Verwendung : Offenlegungsschrift DE10056740A1 ; Offenlegungstag: 23.05.2002 Y1 - 2002 N1 - Volltext über Datenbank: http://publikationen.dpma.de/ PB - Deutsches Patent- und Markenamt CY - München ER - TY - JOUR A1 - Merschenz-Quack, Angelika A1 - Hemmerling, Hans-Jörg A1 - Wunderlich, Hartmut T1 - 1,2-Deoxygenation of vic-Dihydroxyindenoimidazoles: Optimization of a Novel Deoxygenation Reagent. I. / Hemmerling, Hans-Jörg ; Merschenz-Quack, Angelika ; Wunderlich, Hartmut JF - Zeitschrift für Naturforschung B. 59 (2004), H. 10 Y1 - 2004 SN - 0932-0776 SP - 1143 EP - 1152 ER - TY - JOUR A1 - Merschenz-Quack, Angelika A1 - Mootz, D. T1 - Structures of thionyl halides: SOCl2 and SOBr2 / Mootz, D. ; Merschenz-Quack, A. JF - Acta crystallographica / Section C, Crystal structure communications. C 44 (1988), H. 5 Y1 - 1988 SN - 0108-2701 SP - 926 EP - 927 ER - TY - JOUR A1 - Merschenz-Quack, Angelika A1 - Mootz, D. T1 - Structures of sulfuryl halides: SO2F2, SO2CIF and SO2Cl2 / Mootz, D. ; Merschenz-Quack, A. JF - Acta crystallographica / Section C, Crystal structure communications. C 44 (1988), H. 5 Y1 - 1988 SN - 0108-2701 SP - 924 EP - 925 ER - TY - JOUR A1 - Merschenz-Quack, Angelika A1 - Mootz, D. T1 - Structure of 5-sulfosalicylic acid pentahydrate JF - Acta crystallographica / Section C, Crystal structure communications. C 46 (1990), H. 8 Y1 - 1990 SN - 0108-2701 SP - 1478 EP - 1480 ER - TY - JOUR A1 - Jeromin, Günter Erich T1 - Tetrabenzo[a,c,h,j]phenoxazine-18-yl a new stable neutral radical JF - Tetrahedron Letters . 42 (2001), H. 10 Y1 - 2001 SN - 0040-4039 N1 - ISSN der E-Ausg.: 1359-8562 SP - 1863 EP - 1865 ER - TY - BOOK A1 - Jeromin, Günter Erich T1 - Synthesen und chemische Eigenschaften einiger 1-Oxo-1H-pyrrolizine [Oxo-H-pyrrolizine] sowie des 1-Oxo-1H-pyrrolizins [Oxo-H-pyrrolizins] Y1 - 1981 N1 - Heidelberg, Univ., Diss., 1981 ER - TY - JOUR A1 - Jeromin, Günter Erich A1 - Welsch, Volker T1 - Diketene a New Esterification Reagent in the Enzyme-Aided Synthesis of Chiral Alcohols and Chiral Acetoacetic Acid Esters JF - Tetrahedron Letters . 36 (1995), H. 37 Y1 - 1995 SN - 0040-4039 N1 - ISSN der E-Ausg.: 1359-8562 SP - 6663 EP - 6664 ER - TY - JOUR A1 - Jeromin, Günter Erich A1 - Scheidt, Annette T1 - Aliphatic optically active alcohols by enzyme-aided synthesis JF - Tetrahedron Letters . 32 (1991), H. 48 Y1 - 1991 SN - 0040-4039 N1 - ISSN der E-Ausg.: 1359-8562 SP - 7021 EP - 7024 ER - TY - JOUR A1 - Jeromin, Günter Erich A1 - Albertz, Michael T1 - Optically active α-acetoxycarboxylic acids and α-hydroxycarboxylic acids by enzyme-aided syntheses JF - Journal für Praktische Chemie / Chemiker-Zeitung. 334 (1992), H. 6 Y1 - 1992 SN - 1436-9966 N1 - ISSN der E-Ausg.: 1521-3897 ; In der Bibliothek (Jülich) vorhanden unter 63 ZS 024-1992 SP - 526 EP - 528 ER - TY - JOUR A1 - Lassonczyk, Beate T1 - Untersuchung einer Chronosequenz in Nord-Somalia zur Kennzeichnung der Landschaftsgeschichte JF - 14th International Congress of Soil Science : transactions. vol. 5. Kyoto, Japan, August 12 - 18, 1990 = 14ème Congrès International de la Science du Sol = 14. Internationaler Bodenkundlicher Kongreß / International Society of Soil Science Y1 - 1990 N1 - International Congress of Soil Science ; <14 : ; 1990.08.12-18 : ; Kyoto> SP - 370 EP - 371 ER - TY - JOUR A1 - Lassonczyk, Beate A1 - Grupe, M. T1 - Umweltaudit aus der Sicht des Bodenschutzes JF - Umweltschutz-Management und Öko-Auditing / Manfred Sietz ... (Hrsg.) Y1 - 1993 SN - 3-540-56911-1 SP - 133 EP - 141 PB - Springer CY - Berlin ER - TY - JOUR A1 - Lassonczyk, Beate A1 - Grupe, M. T1 - Ökoaudit - ein neues Arbeitsfeld, auch für die Bodenkunde / Grupe, M. ; Lassonczyk, B. JF - Festschrift für Horst Wiechmann / Pfeiffer, Eva-Maria [Hrsg.] Y1 - 1995 N1 - Hamburger bodenkundliche Arbeiten ; 30 SP - 63 EP - 71 PB - Verein zur Förderung der Bodenkunde CY - Hamburg ER - TY - JOUR A1 - Lauth, Jakob A1 - Hoelderich, W. A1 - Wagenblast, G. T1 - Molecular sieves containing azo dyes JF - Zeolites. 15 (1995), H. 2 Y1 - 1995 SN - 0144-2449 SP - 184 ER - TY - JOUR A1 - Lauth, Jakob A1 - Hoelderich, W. A1 - Wagenblast, G. T1 - Crystalline zeolites containing indigo dyes JF - Zeolites. 15 (1995), H. 1 Y1 - 1995 SN - 0144-2449 SP - 86 ER - TY - JOUR A1 - Lauth, Jakob A1 - Christmann, K. A1 - Schwarz, E. T1 - Adsorption geometry of H on RU(1010) image as determined by LEED and HREELS / Christmann, K. ; Lauth, G. ; Schwarz, E. JF - Vacuum. 41 (1990), H. 1-3 Y1 - 1990 SN - 0042-207X SP - 293 EP - 296 ER - TY - JOUR A1 - Lauth, Jakob A1 - Solomun, T. A1 - Hirschwald, W. A1 - Christmann, K. T1 - The interaction of carbon monoxide with a ruthenium(1010) surface JF - Surface Science. 210 (1989), H. 1-2 Y1 - 1989 SN - 0039-6028 SP - 201 EP - 224 ER - TY - JOUR A1 - Lauth, Jakob A1 - Solomun, T. A1 - Hirschwald, W. A1 - Christmann, K. T1 - The interaction of carbon monoxide with a ruthenium(1010) surface JF - Surface Science Letters. 210 (1989), H. 1-2 Y1 - 1989 SN - 0167-2584 SP - A74 EP - A75 ER - TY - BOOK A1 - Merschenz-Quack, Angelika T1 - Untersuchungen an Systemen mit Wasserstoffbrücken / Angelika Merschenz Y1 - 1983 N1 - Düsseldorf, Univ., Dipl.-Arb., 1983 CY - Düsseldorf ER - TY - BOOK A1 - Merschenz-Quack, Angelika T1 - Halogenide und Hydrate von Oxosäuren des Schwefels : Beitr. zur Kristall- u. Strukturchemie Y1 - 1987 N1 - Düsseldorf, Univ., Diss., 1987 CY - Düsseldorf ER - TY - JOUR A1 - Lassonczyk, Beate A1 - Wiechmann, H. T1 - Untersuchungen zur Verlagerung und Immobilisierung löslicher organischer Stoffe in Sandböden JF - Mitteilungen der Deutschen Bodenkundlichen Gesellschaft. 43 (1985) Y1 - 1985 SN - 0343-107x SP - 771 EP - 776 ER - TY - JOUR A1 - Lassonczyk, Beate A1 - Wiechmann, H. T1 - Untersuchungen zum Akkumulationsverhalten löslicher organischer Substanzen in Podsolen JF - Mitteilungen der Deutschen Bodenkundlichen Gesellschaft. 55 (1987) Y1 - 1987 SN - 0343-107x SP - 791 EP - 796 ER - TY - JOUR A1 - Lassonczyk, Beate T1 - Fossile Bodenbildung in Nord-Somalia JF - Mitteilungen der Deutschen Bodenkundlichen Gesellschaft. 59 (1989), H. 2 Y1 - 1989 SN - 0343-107x SP - 927 EP - 930 ER - TY - JOUR A1 - Lassonczyk, Beate T1 - Bodenentwicklung auf Terrassenschottern im ariden Bereich von NO-Somalia JF - Mitteilungen der Deutschen Bodenkundlichen Gesellschaft. 66 (1991), H. 2 Y1 - 1991 SN - 0343-107x SP - 1153 EP - 1156 ER - TY - JOUR A1 - Lassonczyk, Beate A1 - Herms, U. A1 - Filipinski, M. T1 - Arbeitsgruppe "Böden in Schleswig-Holstein" - Ziele, Aufgaben und Aktivitäten JF - Mitteilungen der Deutschen Bodenkundlichen Gesellschaft. 76 (1993), H. 2 Y1 - 1993 SN - 0343-107x SP - 1129 EP - 1131 ER - TY - BOOK A1 - Lassonczyk, Beate T1 - Immobilisierung löslicher organischer Stoffe aus Auflagehorizonten in Sandböden Y1 - 1987 N1 - Bonn, Univ., Diss., 1987 CY - Bonn ER - TY - BOOK A1 - Lassonczyk, Beate T1 - Verbreitung, Genese und Standorteigenschaften von Böden im Algarve / Jahn, Reinhold , K. Stahr und B. Lassonczyk (Hrsg.) Y1 - 1988 SN - 3-7983-1237-0 N1 - Landschaftsentwicklung und Umweltforschung ; 55 PB - Techn. Univ., Univ.-Bibliothek, Abt. Publ. CY - Berlin ER - TY - JOUR A1 - Lassonczyk, Beate A1 - Alaily, F. A1 - Huth, A. A1 - Gensior, A. T1 - Genesis of soils in the arid part of northeast Somalia / F. Alaily, B. Lassonczyk, A.Huth and A. Gensior JF - Berliner geowissenschaftliche Abhandlungen / Reihe A, Geologie und Paläontologie / hrsg. von d. Geowissenschaftlichen Instituten der Freien u. d. Technischen Universität Berlin. 120 A (1990) Y1 - 1990 SN - 0172-8784 SP - 695 EP - 711 ER - TY - JOUR A1 - Lassonczyk, Beate T1 - Characteristics of a fossil soil developed on the basement complex in the northeast Somalia JF - Berliner geowissenschaftliche Abhandlungen / Reihe A, Geologie und Paläontologie / hrsg. von d. Geowissenschaftlichen Instituten der Freien u. d. Technischen Universität Berlin. 120 A (1990) Y1 - 1990 SN - 0172-8784 SP - 713 EP - 718 ER - TY - JOUR A1 - Lassonczyk, Beate A1 - Voigt, B. A1 - Gabriel, B. A1 - Ghod, Mumin M. T1 - Quaternary events at the Horn of Africa / Voigt, B., B. Gabriel, B. Lassonczyk and Mumin M. Ghod JF - Berliner geowissenschaftliche Abhandlungen / Reihe A, Geologie und Paläontologie / hrsg. von d. Geowissenschaftlichen Instituten der Freien u. d. Technischen Universität Berlin. 120 A (1990) Y1 - 1990 SN - 0172-8784 SP - 679 EP - 694 ER - TY - PAT A1 - Lauth, Jakob A1 - Müller, Ulrich A1 - Hölderich, Wolfgang T1 - Verfahren zur Herstellung von Alumo- und Silicoalumophosphaten mit AEL-Struktur unter Verwendung von 1,2-Bis(4-pyridyl)ethan : Offenlegungsschrift DE4131268A1 ; Veröffentlichungstag im Patentblatt: 25.03.1993 / Anmelder: BASF AG, 67063 Ludwigshafen. Erfinder: Ulrich Müller ; Wolfgang Hölderich ; Günter Lauth Y1 - 1993 N1 - Volltext über Datenbank: http://publikationen.dpma.de/ PB - Deutsches Patent- und Markenamt CY - München ER - TY - BOOK A1 - Lauth, Jakob A1 - Hagemeyer, Alfred A1 - Lautensack, Thomas A1 - Deimling, Axel T1 - Katalysator und Verfahren für die katalytische oxidative Dehydrierung von Alkylaromaten = Catalyst and process for the catalytically oxidative dyhydrogenation of alkyl aromatics : Europäische Patentschrift EP0768996B1 ; EP-Patenterteilungsdatum: 12.05.1999 / Anmelder: BASF AG, 67063 Ludwigshafen. Erfinder: Alfred Hagemeyer ; Günter Lauth ; Thomas Lautensack ; Axel Deimling Y1 - 1999 N1 - Volltext über Datenbank: http://publikationen.dpma.de/ ; Internationale Veröffentlichungsnummer: WO 96/01796 PB - Europäisches Patentamt CY - [München u.a.] ER - TY - BOOK A1 - Lauth, Jakob A1 - Trübenbach, Peter A1 - Hagemeyer, Alfred T1 - Monomodale und polymodale Katalysatorträger und Katalysatoren mit engen Porengrössenverteilungen und deren Herstellverfahren : Europäische Patentschrift EP0764467B1 ; EP-Patenterteilungsdatum: 05.02.2003 / Anmelder: BASF AG, 67063 Ludwigshafen. Erfinder: Peter Trübenbach ; Alfred Hagemeyer ; Günter Lauth ... Y1 - 2003 N1 - Volltext über Datenbank: http://publikationen.dpma.de/ PB - Europäisches Patentamt CY - [München u.a.] ER - TY - PAT A1 - Lauth, Jakob A1 - Dingerdissen, Uwe A1 - Trübenbach, Peter A1 - Steuerle, Ulrich T1 - Verfahren zur Herstellung von Aziridinen : Europäische Offenlegungsschrift EP0763528A2 ; Veröffentlichungstag im Patentblatt : 24.04.1997 / Anmelder: BASF AG, 67063 Ludwigshafen. Erfinder: Uwe Dingerdissen ; Peter Trübenbach ; Günter Lauth ; Ulrich Steuerle ... Y1 - 1997 N1 - Volltext über Datenbank: http://publikationen.dpma.de/ PB - Deutsches Patent- und Markenamt CY - München ER - TY - BOOK A1 - Scherer, Ulrich W. T1 - Untersuchungen zur Synthese und chemischen Charakterisierung sehr schwerer Elemente / Ulrich W. Scherer Y1 - 1989 N1 - zugl. Mainz, Univ., Diss. 1989. GSI-Bericht ; 89-18 PB - Gesellschaft für Schwerionenforschung mbH CY - Darmstadt ER - TY - BOOK A1 - Scherer, Ulrich W. T1 - Ultraschnelle Analytik radioaktiver Wirkstoffe : Abschlußbericht zu Nr. 3.2 und Anlage 2 BNBest-BMBF 98 ; Laufzeit des Vorhabens: 1.9.1999 -28.2.2001 / Zuwendungsempfänger: Ulrich W. Scherer Y1 - 2001 N1 - auch als gedruckte Ausgabe veröffentlicht PB - Universitätsbibliothek u. Technische Informationsbibliothek CY - Hannover ER - TY - JOUR A1 - Scherer, Ulrich W. A1 - Kratz, J. V. A1 - Schädel, M. A1 - Brüchle, W. T1 - Lawrencium Chemistry: No Evidence for Oxidation States Lower than 3+ in Aqueous Solution / U.W. Scherer, J.V. Kratz, M. Schädel, W. Brüchle, K.E. Gregorich, R.A. Henderson, D. Lee, M. Nurmia, D.C. Hoffman JF - Inorganica Chimica Acta. 146 (1988) Y1 - 1988 SN - 0020-1693 SP - 249 EP - 254 ER - TY - JOUR A1 - Scherer, Ulrich W. A1 - Brüchle, W. A1 - Schädel, M. A1 - Kratz, J. V. T1 - The Hydration Enthalpies of Md3+ and Lr3+ / W. Brüchle, M. Schädel, U.W. Scherer, J.V. Kratz, K.E. Gregorich, D. Lee, M. Nurmia, R.M. Chasteler, H.L. Hall, R.A. Henderson, D.C. Hoffman JF - Inorganica Chimica Acta. 146 (1988), H. 2 Y1 - 1988 SN - 0020-1693 SP - 267 EP - 276 ER - TY - JOUR A1 - Scherer, Ulrich W. A1 - Türler, A. A1 - Gäggeler, H. W. A1 - Jost, D. T. T1 - Determination of the Partial Electron-Capture- and Spontaneous-Fission Half-Lives of 254No / A. Türler, H.W. Gäggeler, D.T. Jost, P. Armbruster, W. Brüchle, H. Folger, F.P. Heßberger, S. Hofmann, JF - Zeitschrift für Physik A Hadrons and Nuclei. 331 (1988), H. 3 Y1 - 1988 SN - 0939-7922 SP - 363 EP - 364 ER - TY - JOUR A1 - Scherer, Ulrich W. A1 - Heßberger, F. P. A1 - Gäggeler, H. W. A1 - Armbruster, P. T1 - The New Nuclide 225U / F.P. Heßberger, H. Gäggeler, P. Armbruster, W. Brüchle, H. Folger, S. Hofmann, D. Jost, J.V. Kratz, M.E. Leino, G. Münzenberg, V. Ninov, M. Schädel, U.W. Scherer, K. Sümmerer, A. Türler, D. Ackerman JF - Zeitschrift für Physik A Hadrons and Nuclei. 333 (1989), H. 1 Y1 - 1989 SN - 0939-7922 SP - 111 EP - 112 ER - TY - JOUR A1 - Scherer, Ulrich W. A1 - Gäggeler, H. W. A1 - Jost, D. T. A1 - Türler, A. T1 - Cold Fusion Reactions with 48Ca / H.W. Gäggeler, D.T. Jost, A. Türler, P. Armbruster, W. Brüchle, H. Folger, F.P. Heßberger, S. Hofmann, G. Münzenberg, V. Ninov, W. Reisdorf, M. Schädel, K. Sümmerer, J.V. Kratz, U. Scherer, M.E. Leino JF - Nuclear Physics A . 502 (1989), H. 1 Y1 - 1989 SN - 0375-9474 SP - 561 EP - 570 ER - TY - JOUR A1 - Scherer, Ulrich W. A1 - Brüchle, W. A1 - Brügger, M. A1 - Frink, C. T1 - Reactions of 40Ar with 233U,,235U, and 238U at the Barrier / U.W. Scherer, W. Brüchle, M. Brügger, C. Frink, H. Gäggeler, G. Herrmann, J.V. Kratz, K.J. Moody, M. Schädel, K. Sümmerer, N. Trautmann, G. Wirth JF - Zeitschrift für Physik A Hadrons and Nuclei. 335 (1990), H. 4 Y1 - 1990 SN - 0939-7922 SP - 421 EP - 430 ER - TY - JOUR A1 - Scherer, Ulrich W. A1 - Kratz, J. V. A1 - Zimmermann, H. P. A1 - Schädel, M. T1 - Chemical Properties of Element 105 in Aqueous Solutions: Halide Complex Formation and Anion Exchange into Triisooctylamine / J.V. Kratz, H.P. Zimmermann, U.W. Scherer, M. Schädel, W. Brüchle, K.E. Gregorich, C.M. Gannett, H.L. Hall, R.A. Henderson, D.M. L JF - Radiochimica Acta. 48 (1989) Y1 - 1989 SN - 0033-8230 SP - 121 ER - TY - JOUR A1 - Scherer, Ulrich W. A1 - Schädel, M. A1 - Brüchle, W. A1 - Jäger, E. T1 - ARCA II - A New Apparatus for Fast Repetitive HPLC-Separations / M. Schädel, W. Brüchle, E. Jäger, E. Schimpf, J.V. Kratz, U.W. Scherer, H.P. Zimmermann JF - Radiochimica Acta. 48 (1989) Y1 - 1989 SN - 0033-8230 SP - 171 ER - TY - JOUR A1 - Scherer, Ulrich W. A1 - Gober, M. K. A1 - Kratz, J. V. A1 - Zimmermann, H. P. T1 - Chemical Properties of Element 105 in Aqueous Solution: Extractions into Diisobutylcarbinol / M.K. Gober, J.V. Kratz, H.P. Zimmermann, M. Schädel, W. Brüchle, E. Schimpf, K.E. Gregorich, A. Türler, N.J. Hannink, K.R. Czerwinski, B. Kadkhodayan, D.M. Lee, JF - Radiochimica Acta. 57 (1992) Y1 - 1992 SN - 0033-8230 SP - 77 EP - 84 ER - TY - PAT A1 - Jeromin, Günter Erich A1 - Seubert, Bernhard A1 - Beilharz, Helmut A1 - Fickert, Werner [u.a.] T1 - Niedermolekulare Alkalihuminate : Verfahren zur ihrer Herstellung und Verwendung ; Europäische Patentschrift EP0281679B1 ; Veröffentlichungstag im Patentblatt: 01.08.1991 / Anmelder: Rütgerswerke AG, 6000 Frankfurt. Erfinder: Bernhard Seubert ; Helmut Beilharz ; Günter Jeromin ; Werner Fickert ... Y1 - 1991 N1 - Volltext über Datenbank: http://publikationen.dpma.de/ PB - Deutsches Patent- und Markenamt CY - München ER - TY - PAT A1 - Jeromin, Günter Erich A1 - Seubert, Bernhard A1 - Beilharz, Helmut A1 - Fickert, Werner [u.a.] T1 - Verfahren zur Herstellung von niedermolekularen Alkalihuminaten : Europäische Patentschrift EP0281678B1 ; Veröffentlichungstag im Patentblatt: 13.06.1991 / Anmelder: Rütgerswerke AG, 6000 Frankfurt. Erfinder: Bernhard Seubert ; Helmut Beilharz ; Günter Jeromin ; Werner Fickert ... Y1 - 1991 N1 - Volltext über Datenbank: http://publikationen.dpma.de/ PB - Deutsches Patent- und Markenamt CY - München ER - TY - PAT A1 - Jeromin, Günter Erich A1 - Pastorek, Emmerich A1 - Orth, Winfried A1 - Fickert, Werner T1 - Verfahren zur Herstellung von 5-Alkylchinolinsäuren und Mittel zur Durchführung des Verfahrens : Europäische Patentschrift EP0247277B1 ; Veröffentlichungstag im Patentblatt: 17.10.1991 / Anmelder: Rütgerswerke AG, 6000 Frankfurt. Erfinder: Emmerich Pastorek ; Winfried Orth ; Günter Jeromin ; Werner Fickert ... Y1 - 1991 N1 - Volltext über Datenbank: http://publikationen.dpma.de/ PB - Deutsches Patent- und Markenamt CY - München ER - TY - PAT A1 - Jeromin, Günter Erich A1 - Ort, Winfried A1 - Fickert, Werner T1 - Verfahren zur Chlorierung von Alkylseitenketten stickstoffhaltiger Heterocyclen : Europäische Patentschrift EP0204352B1 ; Veröffentlichungstag im Patentblatt: 23.05.1990 / Anmelder: Rütgerswerke AG, 6000 Frankfurt Y1 - 1990 N1 - Volltext über Datenbank: http://publikationen.dpma.de/ PB - Deutsches Patent- und Markenamt CY - München ER - TY - BOOK A1 - Lauth, Jakob A1 - Harreus, Albrecht A1 - Götz, Norbert A1 - Rang, Harald [u.a.] T1 - Preparation O-(2-Hydroxyalkyl)Oximes = of Verfahren zur Herstellung von O-(2-Hydroxy)Oximen : Europäische Patentschrift EP0775107B1 ; Veröffentlichungstag im Patentblatt: 18.11.1999 / Anmelder: BASF AG, 67063 Ludwigshafen. Erfinder: Albrecht Harreus ; Norbert Götz ; Harald Rang ... Günter Lauth Y1 - 1999 N1 - Volltext über Datenbank: http://publikationen.dpma.de/ ; Text in engl. ; Deutsche Übersetzung unter DE69512786T2 ; Internationale Veröffentlichungsnummer: WO 96/04238 PB - Europäisches Patentamt CY - [München u.a.] ER - TY - PAT A1 - Lauth, Jakob A1 - Hölzle, Markus A1 - Hagemeyer, Alfred T1 - Vanadiumhaltiges Katalysatormaterial mit Hydrotalcitstruktur, herstellbar aus Mischhydroxid-Verbindungen oder Mischoxid-Verbindungen von Vanadium mit zweiwertigen Metallen, und Verfahren zur selektiven Oxidation von Kohlenwasserstoffen : Offenlegungsschrift DE19705328A1 ; Veröffentlichungstag im Patentblatt: 13.08.1998 / Anmelder: BASF AG, 67063 Ludwigshafen. Erfinder: Markus Hölzle ; Alfred Hagemeyer ; Günter Lauth Y1 - 1998 PB - Deutsches Marken- und Patentamt CY - München ER - TY - PAT A1 - Lauth, Jakob A1 - Dingerdissen, Uwe A1 - Trübenbach, Peter A1 - Steuerle, Ulrich T1 - Verfahren zur Herstellung von Aziridinen : Offenlegungsschrift DE19533662A1 ; Veröffentlichungstag im Patentblatt: 13.03.1997 / Anmelder: BASF AG, 67063 Ludwigshafen. Erfinder: Uwe Dingerdissen ; Günter Lauth ; Peter Trübenbach ; Günther Steuerle Y1 - 1997 PB - Deutsches Marken- und Patentamt CY - München ER - TY - PAT A1 - Lauth, Jakob A1 - Trübenbach, Peter A1 - Hagemeyer, Alfred A1 - Dingerdissen, Uwe [u.a.] T1 - Monomodale und polymodale Katalysatorträger und Katalysatoren mit engen Porengrößenverteilungen und deren Herstellverfahren : Offenlegungschrift DE19533486A1 ; Veröffentlichungstag im Patentblatt: 13.03.1997 / Anmelder: BASF AG, 67063 Ludwigshafen. Erfinder: Peter Trübenbach ; Alfred Hagemeyer ; Günter Lauth ... Y1 - 1997 N1 - Volltext über Datenbank: http://publikationen.dpma.de/ PB - Deutsches Patent- und Markenamt CY - München ER - TY - PAT A1 - Lauth, Jakob T1 - Titan-Verbindungen mit Hydrotalcit-Struktur : Offenlegungsschrift DE19523053A1 ; Veröffentlichungstag im Patentblatt : 02.01.1997 / Anmelder: BASF AG, 67063 Ludwigshafen Y1 - 1997 N1 - Volltext über Datenbank: http://publikationen.dpma.de/ PB - Deutsches Patent- und Markenamt CY - München ER - TY - PAT A1 - Lauth, Jakob T1 - Titan-Verbindungen mit Hydrotalcit-Struktur : Europäische Offenlegungsschrift EP0749941A1; Veröffentlichungstag im Patentblatt : 06.02.1997 / Anmelder: BASF AG, 67063 Ludwigshafen Y1 - 1997 N1 - Volltext über Datenbank: http://publikationen.dpma.de/ PB - Deutsches Patent- und Markenamt CY - München ER - TY - JOUR A1 - Biselli, Manfred A1 - Schmidt, S. A1 - Büchs, J. A1 - Born, C. T1 - A new correlation for the wall-to-fluid mass transfer in liquid-sol fluidized beds / Schmidt, S. ; Büchs, J. ; Born, C. ; Biselli, M. JF - Chemical Engineering Science. 54 (1999), H. 6 Y1 - 1999 SN - 0009-2509 SP - 829 EP - 839 ER - TY - JOUR A1 - Biselli, Manfred A1 - Hilbert, Ulrike A1 - Jelinek, Nanni A1 - Schmidt, Sebastian T1 - Biotechnologische Aspekte der Kultivierung humaner T-Lymphozyten / Hilbert, Ulrike ; Jelinek, Nanni ; Schmidt, Sebastian ; Biselli, Manfred JF - Chemie Ingenieur Technik. 72 (2000), H. 7 Y1 - 2000 SN - 1522-2640 SP - 746 EP - 750 ER - TY - JOUR A1 - Biselli, Manfred A1 - Noll, Thomas A1 - Mühlsiepen, Heinz A1 - Engels, Ralf T1 - A cell-culture reactor for the on-line evaluation of radiopharmaceuticals : evaluation of the lumped constant of FDG in human glioma cells / Noll, Thomas ; Mühlensiepen, Heinz ; Engels, Ralf ; Hamacher, Kurt ; Papaspyrou, Manfred ; Langen, Karl-Josef ; Bi JF - Journal of Nuclear Medicine. 41 (2000), H. 3 Y1 - 2000 SN - 0022-3123 SP - 556 EP - 564 ER - TY - JOUR A1 - Biselli, Manfred A1 - Jelinek, N. A1 - Schmidt, S. A1 - Hilbert, U. T1 - Neue Bioreaktoren zur Kultivierung hämatopoetischer Zellen / Jelinek, N. ; Schmidt, S. ; Hilbert, U. ; Thoma, S. ; Biselli, M. ; Wandrey, C. JF - Chemie Ingenieur Technik. 73 (2001), H. 7 Y1 - 2001 SN - 0009-286X SP - 894 EP - 898 ER - TY - JOUR A1 - Biselli, Manfred A1 - Hilbert, U. A1 - Jelinek, N. A1 - Schmidt, S. T1 - Bioprocess development for the cultivation of human T-lymphocytes / Hilbert, U. ; Jelinek, N. Schmidt, S. ; Biselli, M. JF - Engineering in Life Sciences. 1 (2001) Y1 - 2001 SN - 1618-0240 SP - 20 EP - 23 ER - TY - JOUR A1 - Biselli, Manfred A1 - Noll, Thomas A1 - Eisenkrätzer, Detlef A1 - Kiesewetter, André T1 - Improved product formation in high density Chinese hamster ovary cell cultures transfected at confluency / Thomas Noll, Detlef Eisenkrätzer, André Kiesewetter, André Dinter, Steffen Zeng, Christian Wandrey, Manfred Biselli und Eric G. Berger JF - Biotechnology Letters. 24 (2002), H. 11 Y1 - 2002 SN - 0141-5492 SP - 861 EP - 866 ER - TY - JOUR A1 - Biselli, Manfred A1 - Jelinek, N. A1 - Schmidt, S. A1 - Hilbert, U. T1 - Novel bioreactors for the ex vivo cultivation of hematopoietic cells / Jelinek, N. ; Schmidt, S. ; Hilbert, U. ; Thoma, S. ; Biselli, M. ; Wandrey, C. JF - Engineering in Life Sciences. 2 (2002), H. 1 Y1 - 2002 SN - 1618-0240 SP - 15 EP - 18 ER - TY - JOUR A1 - Biselli, Manfred A1 - Noll, Thomas A1 - Jelinek, Nanni A1 - Schmidt, Sebastian T1 - Cultivation of Hematopoietic Stem and Progenitor Cells: biochemical Engineering Aspects / Thomas Noll, Nanni Jelinek, Sebastian Schmidt, Manfred Biselli und Christian Wandrey JF - Tools and Applications of Biochemical Engineering Science Y1 - 2002 SN - 3-540-42250-1 N1 - Advances in Biochemical Engineering/Biotechnology. 74 SP - 111 EP - 128 PB - Springer CY - Berlin ER - TY - JOUR A1 - Maggakis-Kelemen, C. A1 - Bork, M. A1 - Kayser, Peter A1 - Biselli, Manfred A1 - Artmann, Gerhard T1 - Biological and mechanical quality of red blood cells cultured from human umbilical cord blood stem cells JF - Medical and biological engineering and computing. 41 (2003), H. 3 Y1 - 2003 SN - 0140-0118 SP - 350 EP - 356 ER - TY - JOUR A1 - Biselli, Manfred A1 - Rechmann, Hanrik A1 - Friedrich, Andrea A1 - Forouzan, Dara T1 - Characterization of photosynthetically active duckweed ( Wolffia australiana ) in vitro culture by Respiration Activity Monitoring System (RAMOS) / Henrik Rechmann, Andrea Friedrich, Dara Forouzan, Stefan Barth, Heide Schnabl, Manfred Biselli und Robert B JF - Biotechnology Letters. 29 (2007), H. 6 Y1 - 2007 SN - 0141-5492 SP - 971 EP - 977 ER - TY - JOUR A1 - Scherer, Ulrich W. A1 - Schädel, M. A1 - Brüchle, W. A1 - Schimpf, E. T1 - Chemical Properties of Element 105 in Aqueous Solution: Cation Exchange Separations with α-Hydroxyisobutyric Acid / M. Schädel, W. Brüchle, E. Schimpf, H.P. Zimmermann, M.K. Gober, J.V. Kratz, N. Trautmann, H. Gäggeler, D. Jost, J. Kovacs, U.W. Sche JF - Radiochimica Acta. 57 (1992) Y1 - 1992 SN - 0033-8230 SP - 85 EP - 92 ER - TY - JOUR A1 - Scherer, Ulrich W. A1 - Gäggeler, H. W. A1 - Jost, D. T. A1 - Kovacs, J. T1 - Gas Phase Chromatography Experiments with Bromides of Tantalum and Element 105 / H.W. Gäggeler, D.T. Jost, J. Kovacs, U.W. Scherer, A. Weber, D. Vermeulen, A. Türler, K.E. Gregorich, R.A. Henderson, K.R. Czerwinski, B. Kadkhodayan, D.M. Lee, M. Nurmia, D. JF - Radiochimica Acta. 57 (1992) Y1 - 1992 SN - 0033-8230 SP - 93 EP - 100 ER - TY - JOUR A1 - Scherer, Ulrich W. A1 - Zimmermann, H. P. A1 - Gober, M. K. A1 - Kratz, J. V. T1 - Chemical Properties of Element 105 in Aqueous Solution: Back Extraction from Triisooctyl Amine into 0.5M HCl / H.P. Zimmermann, M.K. Gober, J.V. Kratz, M. Schädel, E. Schimpf, K.E. Gregorich, A. Türler, K.R. Czerwinski, N.J. Hannink, B. Kadkhodayan, D.M. JF - Radiochimica Acta. 60 (1993) Y1 - 1993 SN - 0033-8230 SP - 11 ER - TY - JOUR A1 - Scherer, Ulrich W. A1 - Tomasberger, T. A1 - Veltkamp, T. C. A1 - Booij, A. S. T1 - Radiocesium Removal from High Level Liquid Waste and Immobilisation in Sodium SilicoTitanate for Geological Disposal / T. Tomasberger, T.C. Veltkamp, A.S. Booij, U.W. Scherer JF - Radiochimica Acta. 89 (2001), H. 3 Y1 - 2001 SN - 0033-8230 SP - 145 EP - 150 ER - TY - JOUR A1 - Scherer, Ulrich W. A1 - Kratz, J. V. A1 - Gober, M. K. A1 - Zimmermann, H. P. T1 - New nuclide 263 105 / J.V. Kratz, M.K. Gober, H.P. Zimmermann, M. Schädel, W. Brüchle, E. Schimpf, K.E. Gregorich, A. Türler, N.J. Hannink, K.R. Czerwinski, B. Kadkhodayan, D.M. Lee, M.J. Nurmia, D.C. Hoffman, H. Gäggeler, D. Jost, U.W. Scherer, A. Weber JF - Physical Review C . 45 (1992) Y1 - 1992 SP - 1064 EP - 1069 ER - TY - JOUR A1 - Scherer, Ulrich W. A1 - Baltensperger, Urs A1 - Ammann, Markus A1 - Bochert, Ulrich K. T1 - Use of 13N for Studies of the Selective Reduction of NO by NH3 over Vanadia/Titania Catalyst at Very Low Reactant Concentrations / Urs Baltensperger, Markus Ammann, Ulrich K. Bochert, Bernd Eichler, Heinz W. Gäggeler, Dieter T. Jost, Joseph A. Kovacs, An JF - Journal of Physical Chemistry. 97 (1993) Y1 - 1993 SN - 0022-3654 SP - 12325 EP - 12330 ER - TY - JOUR A1 - Scherer, Ulrich W. T1 - Zur Radiopharmazie kardialer und kardiovaskulärer Radiopharmazeutika JF - Kardiologische Nuklearmedizin / G. Hör [Hrsg.] Y1 - 1997 SN - 3-609-62970-3 SP - 27 EP - 42 PB - ecomed CY - Landsberg ER - TY - JOUR A1 - Scherer, Ulrich W. A1 - Hör, G. T1 - Kompartimentmodelle zur Quantifizierung des myokardialen Stoffwechsels mit PET / U. W. Scherer, G. Hör JF - Kardiologische Nuklearmedizin / G. Hör [Hrsg.] Y1 - 1997 SN - 3-609-62970-3 SP - 179 EP - 189 PB - ecomed CY - Landsberg ER - TY - JOUR A1 - Scherer, Ulrich W. A1 - Hör, G. T1 - Artifacts and Pitfalls in FDG-PET Whole-Body Scans / U.W. Scherer, G. Hör JF - Radionuclides for Mammary Gland - Current Status and Future Aspects / G. S. Limouris [Hrsg.] Y1 - 1997 SN - 960-85227-6-5 SP - 37 EP - 42 PB - Mediterra Publishers CY - Athen ER - TY - JOUR A1 - Scherer, Ulrich W. A1 - Hör, G. A1 - Hertel, A. T1 - Positronen-Emissions-Tomographie des Herzens / G. Hör, A. Hertel, U. W. Scherer JF - Herzerkrankungen und Interventionsmöglichkeiten / F. Unger [Hrsg.] Y1 - 1998 SN - 3-540-62916-5 SP - 236 EP - 263 PB - Springer CY - Berlin ER - TY - JOUR A1 - Biselli, Manfred A1 - Schmid, Georg A1 - Wandrey, Christian T1 - Preparation of cellodextrins and isolation of oligomeric side components and their characterization / Schmid, Georg ; Biselli, Manfred ; Wandrey, Christian JF - Analytical Biochemistry. 175 (1988) Y1 - 1988 SN - 0003-2697 SP - 573 EP - 583 ER - TY - JOUR A1 - Biselli, Manfred A1 - Thömmes, J. A1 - Garske, U. A1 - Wandrey, Christian T1 - Integrated detoxification: Reduction of ammonium concentration by dialysis with cation exchange membranes / Thömmes, J. ; Garske, U. ; Biselli, M. ; Wandrey, C. JF - Animal cell technology : developments, processes, and products ; ESACT, European Society for Animal Cell Technology, the 11th meeting / Ed. R. E. Spier Y1 - 1992 SN - 0750604212 SP - 171 EP - 175 PB - Butterworth-Heinemann CY - Oxford ER - TY - JOUR A1 - Biselli, Manfred A1 - Hambach, B. A1 - Runstadler, P. W. A1 - Wandrey, Christian T1 - Development of a reactor-integrated aeration system for cultivation of animal cells in fluidized beds / Hambach, B. ; Biselli, M. ; Runstadler, P.W. ; Wandrey, C. JF - Animal cell technology : developments, processes, and products ; ESACT, European Society for Animal Cell Technology, the 11th meeting / Ed. R. E. Spier Y1 - 1992 SN - 0750604212 SP - 381 EP - 385 PB - Butterworth-Heinemann CY - Oxford ER - TY - JOUR A1 - Biselli, Manfred A1 - Lüllau, E. A1 - Dreisbach, C. A1 - Grogg, A. T1 - Immobilization of animal cells on chemically modified siran carrier / Lüllau, E. ; Dreisbach, C. ; Grogg, A. ; Biselli, M. ; Wandrey, C. JF - Animal cell technology : developments, processes, and products ; ESACT, European Society for Animal Cell Technology, the 11th meeting / Ed. R. E. Spier Y1 - 1992 SN - 0750604212 SP - 469 EP - 475 PB - Butterworth-Heinemann CY - Oxford ER - TY - JOUR A1 - Biselli, Manfred A1 - Van der Pol, Jens J. A1 - Joksch, B. A1 - Eberhardt, R. T1 - Development of an enzymatic multichannel flow injection analysis system for monitoring mammalian cell fermentations / Van der Pol, J.J.; Joksch, B.; Eberhardt, R.; Biselli, M.; Wandrey, C., Tramper, J. JF - Biosensors : fundamentals, technologies and applications ; contributions to the BMFT status seminar with international participation May, 12 to 14, 1991, Internationales Bildungs-Centrum Bogensee/Brandenburg, Germany / org. by ZIM-Zentralinstitut für Molekularbiologie, Berlin-Buch and GBF, Braunschweig. Ed. by F. Scheller Y1 - 1992 SN - 3527284370 N1 - GBF Monographs ; 17 SP - 349 EP - 352 PB - VCH CY - Weinheim ER - TY - JOUR A1 - Biselli, Manfred A1 - Thömmes, J. A1 - Gätgens, J. A1 - Runstadler, P. W. T1 - The influence of dissolved oxygen tension on the metabolic activity of an immobilized hybridoma population / Thömmes, J. ; Gätgens, J. ; Biselli, M. ; Runstadler, P.W. ; Wandrey, C. JF - Cytotechnology . 13 (1993), H. 1 Y1 - 1993 SN - 0920-9069 SP - 29 EP - 39 ER - TY - JOUR A1 - Biselli, Manfred A1 - Lüllau, E. A1 - Wandrey, Christian T1 - Growth and metabolism of CHO-cells in porous glass carriers / Lüllau, E. ; Biselli, M. ; Wandrey, C. JF - Animal cell technology : products of today, prospects for tomorrow ; ESACT, European Society for Animal Cell Technology, the 12th meeting / Ed. R. E. Spier Y1 - 1994 SN - 0750618450 N1 - Meeting / European Society for Animal Cell Technology ; 12 SP - 252 EP - 255 PB - Butterworth-Heinemann CY - Oxford ER - TY - JOUR A1 - Biselli, Manfred A1 - Rolef, G. A1 - Dunker, R. A1 - Wandrey, Christian T1 - Optimization of antibody production in a fluidized bed bioreactor / Rolef, G. ; Biselli, M. ; Dunker, R. ; Wandrey, C. JF - Animal cell technology : products of today, prospects for tomorrow ; ESACT, European Society for Animal Cell Technology, the 12th meeting / Ed. R. E. Spier Y1 - 1994 SN - 0750618450 N1 - Meeting / European Society for Animal Cell Technology ; 12 SP - 481 EP - 484 PB - Butterworth-Heinemann CY - Oxford ER - TY - JOUR A1 - Biselli, Manfred A1 - Thömmes, J. A1 - Kula, M.-R. A1 - Wandrey, Christian T1 - Batch kinetic data of hybridoma growth and productivity as a basis for the simulation of antibody production in various culture systems / Thömmes, J. ; Biselli, M. ; Kula, M.-R. ; Wandrey, C. JF - Animal cell technology : products of today, prospects for tomorrow ; ESACT, European Society for Animal Cell Technology, the 12th meeting / Ed. R. E. Spier Y1 - 1994 SN - 0750618450 N1 - Meeting / European Society for Animal Cell Technology ; 12 SP - 513 EP - 517 PB - Butterworth-Heinemann CY - Oxford ER - TY - PAT A1 - Jeromin, Günter Erich A1 - Mang, Thomas T1 - Rührreaktionsgefäß und dessen Verwendung : Offenlegungsschrift DE102004026448A1 ; Offenlegungstag: 15.12.2005 Y1 - 2005 N1 - Volltext über Datenbank DEPATISnet PB - Deutsches Patent- und Markenamt CY - München ER - TY - BOOK A1 - Lauth, Jakob A1 - Müller, Ulrich A1 - Hölderich, Wolfgang T1 - Verfahren zur Herstellung von Alumo- und Silicoalumophosphaten mit AEL-Struktur unter Verwendung von 1,2-Bis(4-pyridyl)ethan : Europäische Patentschrift EP0532993B1 ; Veröffentlichungstag im Patentblatt: 29.04.1993 / Anmelder: BASF AG, 6700 Ludwigshafen. Erfinder: Ulrich Müller ; Wolfgang Hölderich ; Günter Lauth Y1 - 1993 N1 - Volltext über Datenbank: http://publikationen.dpma.de/ PB - Europäisches Patentamt CY - [München u.a.] ER - TY - PAT A1 - Jeromin, Günter Erich A1 - Kruse, Bettina A1 - Breuer, Herbert T1 - Stärkeleim mit vermindertem Wassergehalt : Offenlegungsschrift DE4401529A1 ; Offenlegungstag: 27.01.1995 Y1 - 1995 N1 - Volltext über Datenbank: http://publikationen.dpma.de/ PB - Deutsches Patent- und Markenamt CY - München ER - TY - PAT A1 - Jeromin, Günter Erich T1 - Verfahren zur Herstellung von optisch aktiven Alkoholen und optisch aktiven Kohlensäurediestern mit Hilfe von Lipasen : Offenlegungsschrift DE4009891A1 ; Offenlegungstag: 02.10.1991 Y1 - 1991 N1 - Volltext über Datenbank: http://publikationen.dpma.de/ PB - Deutsches Patent- und Markenamt CY - München ER - TY - PAT A1 - Jeromin, Günter Erich A1 - Seubert, Bernhard A1 - Beilharz, Helmut A1 - Fickert, Werner T1 - Wasserfreie Applikationsform niedermolekularer Alkalihuminate : Offenlegungsschrift DE3709353A1 ; Offenlegungstag: 29.09.1988 / Anmelder: Rütgerswerke AG, 6000 Frankfurt. Erfinder: Bernhard Seubert ; Helmut Beilharz ; Werner Fickert ; Günter Jeromin Y1 - 1988 N1 - Volltext über Datenbank: http://publikationen.dpma.de/ PB - Deutsches Patent- und Markenamt CY - München ER - TY - PAT A1 - Jeromin, Günter Erich A1 - Seubert, Bernhard A1 - Beilharz, Helmut A1 - Fickert, Werner [u.a.] T1 - Verfahren zur Herstellung von niedermolekularen Alkalihuminaten : Offenlegungsschrift DE3707910A1 ; Offenlegungstag: 22.09.1988 / Anmelder: Rütgerswerke AG, 6000 Frankfurt. Erfinder: Bernhard Seubert ; Helmut Beilharz ; Werner Fickert ; Günter Jeromin ... Y1 - 1988 N1 - Volltext über Datenbank: http://publikationen.dpma.de/ PB - Deutsches Patent- und Markenamt CY - München ER - TY - PAT A1 - Jeromin, Günter Erich A1 - Seubert, Bernhard A1 - Beilharz, Helmut A1 - Fickert, Werner [u.a.] T1 - Niedermolekulare Alkalihuminate : Verfahren zu ihrer Herstellung und Verwendung ; Offenlegungsschrift DE3707909A1 ; Offenlegungstag: 22.09.1988 / Anmelder: Rütgerswerke AG, 6000 Frankfurt. Erfinder: Bernhard Seubert ; Helmut Beilharz ; Werner Fickert ; Günter Jeromin ... Y1 - 1988 N1 - Volltext über Datenbank: http://publikationen.dpma.de/ PB - Deutsches Patent- und Markenamt CY - München ER - TY - PAT A1 - Jeromin, Günter Erich A1 - Pastorek, Emmerich A1 - Orth, Winfried A1 - Fickert, Werner T1 - Verfahren zur Herstellung von 5-Alkylchinolinsäuren und Mittel zur Durchführung des Verfahrens : Offenlegungsschrift DE3614756A1 ; Offenlegungstag: 05.11.1987 / Anmelder: Rütgerswerke AG, 6000 Frankfurt. Erfinder: Emmerich Pastorek ; Winfried Orth ; Günter Jeromin ; Werner Fickert Y1 - 1987 N1 - Volltext über Datenbank: http://publikationen.dpma.de/ PB - Deutsches Patent- und Markenamt CY - München ER - TY - PAT A1 - Jeromin, Günter Erich A1 - Ort, Winfried A1 - Fickert, Werner T1 - Verfahren zur Chlorierung von Alkylseitenketten stickstoffhaltiger Heterocyclen : Offenlegungsschrift DE3519364A1 ; Offenlegungstag: 04.12.1986 / Anmelder: Rütgerswerke AG, 6000 Frankfurt Y1 - 1986 N1 - Volltext über Datenbank: http://publikationen.dpma.de/ PB - Deutsches Patent- und Markenamt CY - München ER - TY - PAT A1 - Jeromin, Günter Erich A1 - Dietrich, Wolf A1 - Giebeler, Eberhard A1 - Weiss, Wolfgang T1 - Holzschutzmittel : Europäische Patentschrift EP0293556B1 ; Veröffentlichungstag im Patentblatt: 23.01.1992 / Anmelder: Rütgerswerke AG, 6000 Frankfurt. Erfinder: Wolf Dietrich ; Eberhard Giebeler ; Günter Jeromin ; Wolfgang Weiss Y1 - 1992 N1 - Volltext über Datenbank: http://publikationen.dpma.de/ PB - Deutsches Patent- und Markenamt CY - München ER - TY - PAT A1 - Jeromin, Günter Erich A1 - Seubert, Bernhard A1 - Beilharz, Helmut A1 - Fickert, Werner [u.a.] T1 - Wasserfreie Applikationsform niedermolekularer Alkalihuminate : Europäische Patentschrift EP0283573B1 ; Veröffentlichungstag im Patentblatt: 09.04.1992 / Anmelder: Rütgerswerke AG, 6000 Frankfurt. Erfinder: Bernhard Seubert ; Helmut Beilharz ; Günter Jeromin ; Werner Fickert ... Y1 - 1992 N1 - Volltext über Datenbank: http://publikationen.dpma.de/ PB - Deutsches Patent- und Markenamt CY - München ER - TY - PAT A1 - Lauth, Jakob A1 - Dingerdissen, Uwe A1 - Steuerle, Ulrich T1 - Schalenkatalysator und Verfahren zur Herstellung von Aziridinen : Offenlegungsschrift DE19514146A1 ; Veröffentlichungstag im Patentblatt : 17.10.1996 / Anmelder: BASF AG, 67063 Ludwigshafen. Erfinder: Uwe Dingerdissen ; Günter Lauth ; Ulrich Steuerle Y1 - 1996 N1 - Volltext über Datenbank: http://publikationen.dpma.de/ PB - Deutsches Patent- und Markenamt CY - München ER - TY - PAT A1 - Lauth, Jakob A1 - Burkhart, Bernd A1 - Oftring, Alfred T1 - Verfahren zur Herstellung von Alkoxylierungsprodukten mit verbesserter Filtrierbarkeit : Offenlegungsschrift DE19505037A1 ; Veröffentlichungstag im Patentblatt : 22.08.1996 / Anmelder: BASF AG, 67063 Ludwigshafen. Erfinder: Bernd Burghart ; Günter Lauth ; Alfred Oftring Y1 - 1996 N1 - Volltext über Datenbank: http://publikationen.dpma.de/ PB - Deutsches Patent- und Markenamt CY - München ER - TY - PAT A1 - Lauth, Jakob A1 - Dingerdissen, Uwe A1 - Henne, Andreas T1 - Verfahren zur Herstellung von Aminen : Offenlegungsschrift DE4431093A1 ; Veröffentlichungstag im Patentblatt : 07.03.1996 / Anmelder: BASF AG, 67063 Ludwigshafen. Erfinder: Uwe Dingerdissen ; Günter Lauth ; Andreas Henne ... Y1 - 1996 N1 - Volltext über Datenbank: http://publikationen.dpma.de/ PB - Deutsches Patent- und Markenamt CY - München ER - TY - PAT A1 - Lauth, Jakob A1 - Hagemeyer, Alfred A1 - Lautensack, Thomas A1 - Deimling, Axel T1 - Katalysator und Verfahren für die katalytische oxidative Dehydrierung von Alkylaromaten und Paraffinen : Offenlegungsschrift DE4423975A1 ; Veröffentlichungstag im Patentblatt : 11.01.1996 / Anmelder: BASF AG, 67063 Ludwigshafen. Erfinder: Alfred Hagemeyer ; Günter Lauth ; Thomas Lautensack ; Axel Deimling Y1 - 1996 N1 - Volltext über Datenbank: http://publikationen.dpma.de/ PB - Deutsches Patent- und Markenamt CY - München ER - TY - PAT A1 - Lauth, Jakob A1 - Dingerdissen, Uwe A1 - Harth, Klaus A1 - Steuerle, Ulrich T1 - Schalenkatalysator, seine Verwendung bei der Herstellung von Aziridinen und Verfahren zur Herstellung von Aziridinen : Offenlegungsschrift DE4409310A1 ; Veröffentlichungstag im Patentblatt : 21.09.1995 / Anmelder: BASF AG, 67063 Ludwigshafen. Erfinder: Uwe Dingerdissen ; Günter Lauth ; Klaus Harth ; Ulrich Steuerle Y1 - 1995 N1 - Volltext über Datenbank: http://publikationen.dpma.de/ PB - Deutsches Patent- und Markenamt CY - München ER - TY - PAT A1 - Lauth, Jakob A1 - Wolf, Gerhard A1 - Burkhart, Bernd T1 - Verfahren zur Herstellung von Alkoxylierungsprodukten in Gegenwart von mit Additiven modifizierten Mischhydroxiden ; Offenlegungsschrift DE4325237A1 : Tag der Veröffentlichung im Patentblatt 02.02.1995 : BASF AG, 67063 Ludwigshafen. Erfinder: Gerhard Wolf ; Bernd Burghart ; Günter Lauth ... Y1 - 1995 N1 - Volltext über Datenbank: http://publikationen.dpma.de/ PB - Deutsches Patent- und Markenamt CY - München ER - TY - PAT A1 - Lauth, Jakob A1 - Hölderich, Wolfgang A1 - Harth, Klaus A1 - Hibst, Hartmut T1 - Schalenkatalysatoren : Offenlegungsschrift: DE4221011A1 ; Veröffentlichungstag im Patentblatt: 05.01.1994 / Anmelder: BASF AG, 67063 Ludwigshafen Y1 - 1994 N1 - Volltext über Datenbank: http://publikationen.dpma.de/ PB - Deutsches Patent- und Markenamt CY - München ER - TY - PAT A1 - Lauth, Jakob A1 - Hölderich, Wolfgang A1 - Wagenblast, Gerhard A1 - Albert, Bernhard [u.a.] T1 - Azofarbstoffe enthaltende Molekularsiebe : Offenlegungsschrift DE4207745A1 ; Veröffentlichungstag im Patentblatt: 16.09.1993 / Anmelder: BASF AG, 67063 Ludwigshafen Y1 - 1993 N1 - Volltext über Datenbank: http://publikationen.dpma.de/ PB - Deutsches Patent- und Markenamt CY - München ER - TY - PAT A1 - Lauth, Jakob A1 - Hölderich, Wolfgang A1 - Wagenblast, Gerhard A1 - Schefczik, Ernst T1 - Molekularsiebe, enthaltend Farbstoffe auf Basis von Indigo : Offenlegungsschrift DE4207339A1 ; Veröffentlichungstag im Patentblatt: 16.09.1993 / Anmelder: BASF AG, 67063 Ludwigshafen Y1 - 1993 N1 - Volltext über Datenbank: http://publikationen.dpma.de/ PB - Deutsches Patent- und Markenamt CY - München ER - TY - PAT A1 - Lauth, Jakob A1 - Müller, Ulrich A1 - Hölderich, Wolfgang A1 - Brode, Stefan [u.a.] T1 - Farbige, kristalline Alumophosphate und/oder Silicoalumophosphate vom AEL-Typ : Offenlegungsschrift DE4131447A1 ; Veröffentlichungstag im Patentblatt: 25.03.1993 / Anmelder: BASF AG, 67063 Ludwigshafen Y1 - 1993 N1 - Volltext über Datenbank: http://publikationen.dpma.de/ PB - Deutsches Patent- und Markenamt CY - München ER - TY - BOOK A1 - Rath, Walter A1 - Dzierzynski, Elmar T1 - Solvent-free contact adhesive = Lösungsmittelfreier Kontaktklebstoff / Rath, Walter ; Dzierzynski, Elmar [Erfinder] Y1 - 1995 N1 - EP0436347 06.04.1995 Veröffentlichungstag im Patentblatt ; Volltext recherchierbar über: PB - Europäisches Patentamt CY - München ER - TY - PAT A1 - Rath, Walter A1 - Seitz, Karsten T1 - Frühregenfeste Dichtmasse auf Basis wässriger Acrylatdispersionen und Verfahren zu ihrer Herstellung Y1 - 1993 N1 - DE000004205358A1 Veröffentlichungstag im Patentblatt 26.08.1993 ; Volltext recherchierbar über PB - Deutsches Patent- und Markenamt CY - München ER - TY - PAT A1 - Rath, Walter A1 - Seitz, Karsten T1 - Frühregenfeste Dichtmasse auf Basis wässriger Acrylatdispersionen und Verfahren zu ihrer Herstellung Y1 - 1993 N1 - DE000004224044A1 Veröffentlichungstag im Patentblatt 27.01.1994 ; Volltext recherchierbar über PB - Deutsches Patent- und Markenamt CY - München ER - TY - BOOK A1 - Rath, Walter A1 - Koch, Markus A1 - Reichardt, Rüdiger T1 - Composition de polyurethanne et joint d’etancheite pour vitrage = Polyurethanzusammensetzung und Verglasungsdichtungs-Profil / Rath, Walter, Koch, Markus ; Reichardt, Rüdiger [Erfinder] Y1 - 2002 N1 - EP0789719 24.01.2002 Veröffentlichungstag im Patentblatt ; Volltext recherchierbar über PB - Europäisches Patentamt CY - München ER - TY - BOOK A1 - Baüer, Herbert A1 - Gilch, Heinz Gerhard A1 - Rath, Walter A1 - Schümann, Uwe T1 - Moisture-curing polyurethane-based hot-melt compositions = Feuchthärtbare Heisschmelz-Zusammensetzungen auf Polyurethanbasis Y1 - 1990 N1 - EP0386879 18.10.1990 Veröffentlichungstag im Patentblatt ; Volltext recherchierbar über PB - Europäisches Patentamt CY - München ER - TY - PAT A1 - Mang, Thomas A1 - Maier, Josef T1 - Verfahren zur Herstellung einer haptenisierten Polysaccharidmatrix Y1 - 1989 N1 - DE3744068A1 06.07.1989 Veröffentlichungstag im Patentblatt ; Volltext recherchierbar über PB - Deutsches Patent- und Markenamt CY - München ER - TY - BOOK A1 - Mang, Thomas T1 - Verfahren zur Herstellung einer haptenisierten Polysaccharidmatrix Y1 - 1989 N1 - EP0322742 10.08.1989 Veröffentlichungstag im Patentblatt ; Volltext recherchierbar über PB - Europäisches Patentamt CY - München ER - TY - PAT A1 - Mang, Thomas A1 - Maier, Josef T1 - Verfahren zur Herstellung einer Festphasenmatrix, an die Haptene kovalent gebunden sind Y1 - 1990 N1 - DE 38 41 566 13.06.1990 Veröffentlichungstag im Patentblatt ; Volltext recherchierbar über PB - Deutsches Patent- und Markenamt CY - München ER - TY - BOOK A1 - Mang, Thomas A1 - Maier, Josef T1 - Verfahren zur Herstellung einer Festphasenmatrix, an die Haptene kovalent gebunden sind Y1 - 1990 N1 - EP0372581 19.07.1990 Veröffentlichungstag im Patentblatt ; Volltext recherchierbar über PB - Europäisches Patentamt CY - München ER - TY - JOUR A1 - Biselli, Manfred A1 - Wandrey, Christian T1 - Reaction Mechanism of an Endoglucanase of Trichoderma Reesei QM 9414 using Cellodextrins as Substrate JF - Technology of biological processes, safety in biotechnology, applied genetic engineering : lectures held at the 5th DECHEMA Annual Meeting of Biotechnologists, May 12 - 13, 1987, [Frankfurt, Main] / [hrsg. von Dieter Behrens ...] Y1 - 1988 N1 - Jahrestagung der Biotechnologen ; <5, 1987, Frankfurt, Main> ; DECHEMA Biotechnology Conferences series ; 1 SP - 425 EP - 429 PB - VCH CY - Weinheim ER - TY - BOOK A1 - Mang, Thomas T1 - Verfahren zum Trennen eines Kunststoffes von einem Trägermaterial / Offenlegungsschrift DE 4313007 A1 ; Offenlegungstag 27.10.1994/ Anmelder und Erfinder : Mang, Thomas Y1 - 1994 N1 - DE 4313007 A1 ; Veröffentlichungstag im Patentblatt 27.10.1994 ; Volltext recherchierbar über PB - Deutsches Patentamt CY - München ER - TY - BOOK A1 - Mang, Thomas A1 - Haulena, Friedhelm T1 - Vorrichtung zur Aufhebung der Verbindung verschiedener Komponenten eines Verbundwerkstoffes / Patentschrift DE 4320000 C1 Veröffentlichungstag der Patenterteilung 11.05.1994 / Erfinder : Rose, Gerd ; Haulena, Friedhelm ; Mang, Thomas M. ; Patentinhaber: Schering AG Y1 - 1994 N1 - DE 4320000 C1 ; Volltext recherchierbar über PB - Deutsches Patentamt CY - München ER - TY - PAT A1 - Lauth, Jakob A1 - Hagemeyer, Alfred A1 - Lautensack, Thomas A1 - Deimling, Axel T1 - Catalyst and catalytic oxidative dehydrogenation of alkylaromatics and paraffins : United States Patent ; patent number 5,902,918 ; date of patent: May 11, 1999 / Assignee: BASF Aktiengesellschaft. Inventors: Alfred Hagemeyer ; Gunter Lauth ; Thomas Lautensack ; Axel Deimling Y1 - 1999 N1 - Barcode: US005902918A ; Volltext über Datenbank: http://www.google.com/patents ; Falsche Namensansetzung: Gunter ... PB - United States Trademark and Patent Office CY - [Alexandria, VA] ER - TY - BOOK A1 - Lauth, Jakob A1 - Müller, Ulrich A1 - Hoelderich, Wolfgang T1 - Farbige, kristalline Alumophosphate und/oder Silicoalumophosphate vom AEL-Typ oder VFI-Typ ; Europäische Patentanmeldung EP0534289A1 ; Veröffentlichungstag im Patentblatt: 06.05.1993 / BASF AG, 6700 Ludwigshafen. Erfinder: Günther Lauth; Ulrich Müller ; Wolfgang Hoelderich ... Y1 - 1993 N1 - Volltext über Datenbank http://publikationen.dpma.de/ ; Falsche Namenansersetzung in Datenbank: Günther PB - Europäisches Patentamt CY - [München u.a.] ER - TY - BOOK A1 - Lauth, Jakob A1 - Hölderich, Wolfgang A1 - Wagenblast, Gerhard T1 - Azofarbstoffe enthaltende Molekularsiebe = Molecular sieves containing azo dyes ; Europäische Patentschrift EP0630354B1 ; Veröffentlichungsdatum: 13.11.1996 / Anmelder: BASF AG Y1 - 1996 N1 - Volltext über Datenbank http://depatisnet.dpma.de/ ; Internationale Veröffentlichungsnummer: WO 93/17965 PB - Europäisches Patentamt CY - [München u.a.] ER - TY - BOOK A1 - Lauth, Jakob A1 - Hoelderich, Wolfgang A1 - Wagenblast, Gerhard T1 - Molecular sieves containing azo dyes ; Internationale [Pantent]anmeldung WO93/17965 ; Veröffentlichungsdatum: 16.09.1993 / Anmelder: BASF AG Y1 - 1993 N1 - Volltext über Datenbank http://depatisnet.dpma.de/ PB - Weltorganisation für geistiges Eigentum, Internat. Büro CY - [Genf] ER - TY - BOOK A1 - Lauth, Jakob A1 - Hoelderich, Wolfgang A1 - Wagenblast, Gerhard T1 - Molekularsiebe, enthaltend Farbstoffe auf Basis von Indigo = Molecular sieves containing indigo-based dyes ; Europäische Patenanmeldung EP0630353A1 ; Veröffentlichungsdatum 28.12.1994 / Anmelder: BASF AG Y1 - 1994 N1 - Internationale Anmeldung veröffentlicht durch die Weltorganisation für geistiges Eigentum unter der Nummer WO 93/17966 PB - Europäisches Patentamt CY - [München u.a.] ER - TY - BOOK A1 - Lauth, Jakob A1 - Hoelderich, Wolfgang A1 - Wagenblast, Gerhard T1 - Molekularsiebe, enthaltend Farbstoffe auf Basis von Indigo = Molecular sieves containing indigo-based dyes ; Internationale [Pantent]anmeldung WO 93/17966 ; Veröffentlichungsdatum 16.09.1993 / Anmelder: BASF AG Y1 - 1993 N1 - Volltext über Datenbank http://depatisnet.dpma.de/ PB - Weltorganisation für geistiges Eigentum, Internat. Büro CY - [Genf] ER - TY - BOOK A1 - Lauth, Jakob A1 - Hoelderich, Wolfgang A1 - Harth, Klaus A1 - Hirbst, Hartmut T1 - Schalenkatalysatoren = Shell coated catalysts ; Europäische Patentschrift EP0576944B1 ; Veröffentlichungsdatum 26.02.1997 / Anmelder: BASF Aktiengesellschaft Y1 - 1997 N1 - Volltext über Datenbank: http://depatisnet.dpma.de/ PB - Europäisches Patentamt CY - [München u.a.] ER - TY - BOOK A1 - Lauth, Jakob A1 - Gerhard, Wolf A1 - Burghart, Bernd T1 - Verfahrung zur Herstellung von Alkoxylierungsprodukten in Gegenwart von mit additiven modifizierten Mischhydroxiden = Method for preparing alkoxylation products in the presence of additive-modified mixed hydoxides ; Europäische Patentschrift EP0711268B1 ; Veröffentlichungsdatum 28.10.1998 / Anmelder: BASF AG. Erfinder: Wolf Gerhard ; Bernd Burghart ; Günter Lauth ... Y1 - 1998 N1 - Volltext über Datenbank: http://depatisnet.dpma.de/ ; Internationale Veröffentlichungsnummer: WO 95/04024 PB - Europäisches Patentamt CY - [München u.a.] ER - TY - PAT A1 - Lauth, Jakob A1 - Gerhard, Wolf A1 - Burghart, Bernd T1 - Verfahrung zur Herstellung von Alkoxylierungsprodukten in Gegenwart von mit Additiven modifizierten Mischhydroxiden = Method for preparing alkoxylation products in the presence of additive-modified mixed hydoxides ; Internationale [Patent]anmeldung WO 95/04024 ; Veröffentlichungsdatum 09.02.1995 / Anmelder: BASF AG ... Erfinder: Wolf Gerhard ; Bernd Burghart ; Günter Lauth ... N2 - Herstellung von Alkoxylierungsprodukten durch Umsetzung von Verbindungen mit aktiven Wasserstoffatomen mit C2- bis C4-Alkylenoxiden in Gegenwart eines mit Additiven modifizierten, aus Polykationen aufgebauten Mischhydroxids der allgemeinen Formel (I): [M(II)1-xM(III)x(OH)2]Ax/n . m L oder (II): [LiA12(OH)6]A1/n . m L in denen M(II) mindestens ein zweiwertiges Metallion, M(III) mindestens ein dreiwertiges Metallion, A mindestens ein anorganisches Anion und L ein organisches Lösungsmittel oder Wasser bedeutet, n die Wertigkeit des anorganischen Anions A oder bei mehreren Anionen A deren mittlere Wertigkeit bezeichnet und x einen Wert von 0,1 bis 0,5 und m einen Wert von 0 bis 10 annehmen kann, als Alkoxylierungskatalysator, wobei das Mischhydroxid als Additive (a) aromatische oder heteroaromatische Mono- oder Polycarbonsäuren oder deren Salze, (b) aliphatische Mono- oder Polycarbonsäuren oder deren Salze mit einem isocyclischen oder heterocyclischen Ring in der Seitenkette, (c) Halbester von Dicarbonsäuren oder deren Salze, (d) Carbonsäureanhydride, (e) aliphatische oder aromatische Sulfonsäuren oder deren Salze, (f) C8- bis C18-Alkylsulfate, (g) langkettige Paraffine, (h) Polyetherole oder Polyetherpolyole oder (j) Alkohole oder Phenole, welche nicht zwischen den Schichten des Mischhydroxids (I) oder (II) eingebaut sind, enthält. Y1 - 1995 PB - Weltorganisation für geistiges Eigentum, Internat. Büro CY - [Genf] ER - TY - PAT A1 - Lauth, Jakob A1 - Hagemeyer, Alfred A1 - Lautensack, Thomas A1 - Deimling, Axel T1 - Katalysator und Verfahren für die katalytische oxidative Dehydrierung von Alkylaromaten und Paraffinen = Catalyst and process for the catalytically oxidative dyhydrogenation of alkyl aromatics and paraffins : Internationale [Patent]anmeldung WO 96/001796 ; Veröffentlichungsdatum: 25.01.1996 / Anmelder: BASF AG, 67063 Ludwigshafen. Erfinder: Alfred Hagemeyer ; Günter Lauth ; Thomas Lautensack ; Axel Deimling Y1 - 1996 N1 - Volltext über Datenbank http://depatisnet.dpma.de/ PB - Weltorganisation für geistiges Eigentum, Internat. Büro CY - [Genf] ER - TY - JOUR A1 - Scherer, Ulrich W. A1 - Hör, G. A1 - Kranert, W. T. A1 - Maul, F. D. T1 - Gated Metabolic Positron Emission Tomography (GAPET) of Myocardium: 18F-FDG/PET to optimize Recognition of Myocardial Hibernation / G. Hör, W.T. Kranert, F.D. Maul, O. Schröder, A. Karimian-Tatriz, O. Geb, R.P. Baum, U.W. Scherer JF - Nuclear Medicine Communications. 19 (1998) Y1 - 1998 SN - 0143-3636 SP - 535 EP - 545 ER - TY - JOUR A1 - Scherer, Ulrich W. A1 - Srivastava, Alok A1 - Singh, Vivendra A1 - Chandra, Amita T1 - Electrical conductivity studies of swift heavy ion modified PVC and PVC-PANI composite / Alok Srivastava ,Virendra Singh, Amita Chandra, K.Witte, U.W.Scherer and T.V.Singh JF - Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms. 245 (2006), H. 1 Y1 - 2006 SN - 0168-583X SP - 277 EP - 280 ER - TY - JOUR A1 - Turek, Monika A1 - Ketterer, Lothar A1 - Claßen, Melanie A1 - Berndt, Heinz A1 - Elbers, Gereon A1 - Krüger, Peter A1 - Keusgen, Michael A1 - Schöning, Michael Josef T1 - Development and Electrochemical Investigations of an EIS-(Electrolyte-Insulator-Semiconductor) based Biosensor for Cyanide Detection JF - Sensors Y1 - 2007 SN - 1424-8220 VL - 7 IS - 8 SP - 1415 EP - 1426 ER - TY - JOUR A1 - Scherer, Ulrich W. A1 - Türler, A. A1 - Gäggeler, H. W. A1 - Gregorich, K. E. T1 - Gas phase chromatography of halides of elements 104 and 105 / A. Türler, H. W. Gäggeler, K. E. Gregorich, H. Barth, W. Brüchle, K. R. Czerwinski, M. K. Gober, N. J. Hannink, R. A. Henderson, D. C. Hoffman, D. T. Jost, C. D. Kacher, B. Kadkhodayan, J. Kova JF - Journal of Radioanalytical and Nuclear Chemistry. 160 (1992), H. 2 Y1 - 1992 SN - 0236-5731 SP - 327 EP - 339 ER - TY - JOUR A1 - Scherer, Ulrich W. T1 - Controlled ion track etching / J. George; M. Irkens ; S. Neumann ; U. W. Scherer ; A. Srivastava ; D. Sinha ; D. Fink JF - Radiation Effects and Defects in Solids. 161 (2006), H. 3 Y1 - 2006 SP - 161 EP - 175 ER - TY - JOUR A1 - Wagner, Torsten A1 - Molina, Roberto A1 - Yoshinobu, Tatsuo A1 - Kloock, Joachim P. A1 - Biselli, Manfred A1 - Canzoneri, Michele A1 - Schnitzler, Thomas A1 - Schöning, Michael Josef T1 - Handheld multi-channel LAPS device as a transducer platform for possible biological and chemical multi-sensor applications JF - Electrochimica Acta. 53 (2007), H. 2 Y1 - 2007 SN - 0013-4686 SP - 305 EP - 311 ER - TY - JOUR A1 - Canzoneri, M. A1 - Krüger, R. A1 - Zang, Werner A1 - Biselli, Manfred T1 - Atmungsaktivität von Säugerzellen: Kontinuierliche Onlineermittlung im Schüttelkolben JF - BIOforum. 3 (2006) Y1 - 2006 SN - 0940-0079 SP - 45 EP - 47 ER - TY - JOUR A1 - Biselli, Manfred A1 - Van der Pol, Jens J. A1 - Spohn, Uwe A1 - Eberhardt, Rolf T1 - On-line monitoring of an animal cell culture with multi-channel flow injection analysis / van der Pol, Jens J.; Spohn, Uwe ; Eberhardt, Rolf ; Gätgens, Jochen ; Biselli, Manfred ; Wandrey, Christian ; Tramper, Johannes JF - Journal of Biotechnology. 37 (1994), H. 3 Y1 - 1994 SN - 0168-1656 SP - 253 EP - 264 ER - TY - JOUR A1 - Biselli, Manfred A1 - Van der Pol, Jens J. A1 - Joksch, B. A1 - Spohn, U. T1 - On-line monitoring and control of glucose, glutamine, lactate and ammonium during a high-cell-density cultivation of hybridoma cells / van der Pol, Jens J. ; Joksch, B. ; Spohn, U. ; Biselli, M. ; Wandrey, C. JF - Proceedings of the sixth international meeting of the Japanese Association for Animal Cell Technology [JAACT 93]: Nagoya, Japan, November 9 - 12, 1993 / ed. by T. Kobayashi Y1 - 1994 SN - 0-7923-3156-7 N1 - International meeting of the Japanese Association for Animal Cell Technology, JAACT 93 ; (6 : ; 1993.11.09-12 : ; Nagoya, Japan) ; Animal cell technology, basic and applied aspects ; 6 SP - 167 EP - 170 PB - Springer Netherland CY - Berlin ER - TY - JOUR A1 - Biselli, Manfred A1 - Kragl, U. A1 - Wandrey, C. T1 - Reaction Engineering for Enzyme-Catalyzed Biotransformations JF - Enzyme catalysis in organic synthesis : a comprehensive handbook. Bd. 1. - 2., completely rev. and enl. ed. / ed. by Karlheinz Drauz. Y1 - 2002 SN - 3-527-29949-1 N1 - erschienen: Vol. 1-3 SP - 185 EP - 257 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Biselli, Manfred A1 - Zeng, Steffen A1 - Dinter, Andre A1 - Eisenkrätzer, Detlef T1 - Pilot Scale Expression and Purification of Soluble Protein A Tagged β1,6N-Acetylglucosaminyltransferase in CHO Cells / Zeng, Steffen ; Dinter, Andre ; Eisenkrätzer, Detlef ; Biselli, Manfred ; Wandrey, Christian ; Berger, Eric G. JF - Biochemical and Biophysical Research Communications. 237 (1997), H. 3 Y1 - 1997 SN - 0006-291X SP - 653 EP - 658 ER - TY - JOUR A1 - Biselli, Manfred A1 - Born, C. A1 - Wandrey, C. T1 - Oxygen transfer from the gasphase to the immobilized cells in membrane aerated fluidized beds / Born, C. ; Biselli, M. ; Wandrey, C. JF - Animal cell technology : basic & applied aspects : proceedings of the Eighth Annual Meeting of the Japanese Association for Animal Cell Technology, Iizuka, Fukuoka, Japan, November 6-10, 1995 / edited by K. Funatsu, Y. Shirai, and T. Matsushita Y1 - 1995 SN - 0-7923-4486-3 N1 - Japanese Association for Animal Cell Technology. ; Meeting ; <8 ; 1995 : ; Iizuka-shi, Japan> SP - 83 EP - 87 PB - Kluwer Acad. Press CY - Boston ER - TY - JOUR A1 - Biselli, Manfred A1 - Schröder, B. A1 - Herfurth, C. A1 - Schmoll, H.-J. T1 - Cocultivation of hematopoietic cells in a fluidized bed reactor / Schröder, B. ; Herfurth, C. ; Biselli, M. ; Schmoll, H.-J. ; Link, H. ; Ebell, W. ; Wandrey, C. JF - Animal cell technology : basic & applied aspects : proceedings of the Eighth Annual Meeting of the Japanese Association for Animal Cell Technology, Iizuka, Fukuoka, Japan, November 6-10, 1995 / edited by K. Funatsu, Y. Shirai, and T. Matsushita Y1 - 1997 SN - 0-7923-4486-3 N1 - Japanese Association for Animal Cell Technology. ; Meeting ; <8 ; 1995 : ; Iizuka-shi, Japan> SP - 137 EP - 141 PB - Kluwer Acad. Press CY - Boston ER - TY - JOUR A1 - Biselli, Manfred A1 - Klyushnichenko, Vadim A1 - Rodenbrock, Anja A1 - Thömmes, Jörg T1 - Analysis of Hybridoma Cell Culture Processes by SDS-Gel Capillary Electrophoresis and matrix-assisted laser desorption ionization–time-of-flight MS / Vadim Klyushnichenko, Anja Rodenbrock, Jörg Thömmes, Maria-Regina Kula, Holger Heine, Manfred Biselli JF - Biotechnology and Applied Biochemistry. 27 (1998), H. 3 Y1 - 1998 SN - 0885-4513 SP - 181 EP - 188 ER - TY - JOUR A1 - Biselli, Manfred A1 - Noll, T. T1 - Dielectric spectroscopy in the cultivation of suspended and immobilized hybridoma cells / Noll, T.; Biselli, M. JF - Journal of Biotechnology. 63 (1998), H. 3 Y1 - 1998 SN - 0168-1656 SP - 187 EP - 198 ER - TY - JOUR A1 - Biselli, Manfred A1 - Jelinek, N. A1 - Schmidt, S. A1 - Noll, T. T1 - Bioreaktoren zur Kultivierung blutbildender Zellen / Jelinek, N. ; Schmidt, S. ; Noll, T. ; Biselli, M. JF - Bioforum. 24 (2001), H. 6 Y1 - 2001 SN - 0940-0079 SP - 418 EP - 419 ER - TY - JOUR A1 - Biselli, Manfred A1 - Hilbert, U. A1 - Noll, T. T1 - Cultivation of Human HCMV Specific Lymphocytes – An Example for Adoptive Immunotherapy / Hilbert, U. ; Biselli, M. ; Noll, T. JF - Animal cell technology : from target to market ; Tylösand, Sweden, June 10 - 14, 2001 / ed. by E. Lindner-Olsson ... Y1 - 2001 SN - 1-4020-0264-5 N1 - Proceedings of the ... ESACT meeting ; 17 SP - 558 EP - 561 PB - Kluwer CY - Dordrecht ER - TY - PAT A1 - Lauth, Jakob A1 - Dingerdissen, Uwe A1 - Harth, Klaus A1 - Steuerle, Ulrich T1 - Schalenkatalysator, seine Verwendung bei der Herstellung von Aziridinen und Verfahren zur Herstellung von Aziridinen : Europäische Offenlegungsschrift EP0750525 ; Veröffentlichungstag im Patentblatt : 13.02.1997 / Anmelder: BASF AG, 67063 Ludwigshafen. Erfinder: Uwe Dingerdissen ; Günter Lauth ; Klaus Harth ; Ulrich Steuerle Y1 - 1997 N1 - Internationale Anmeldung veröffentlicht durch die Weltorganisation für geistiges Eigentum unter der Nummer WO 97/25590 PB - Deutsches Patent- und Markenamt CY - München ER - TY - BOOK A1 - Lauth, Jakob A1 - Dingerdissen, Uwe A1 - Henne, Andreas T1 - Verfahren zur Herstellung von Aminen : Europäische Patentschrift EP0699653B1 ; EP-Patenterteilungsdatum: 07.01.1998 / Anmelder: BASF AG, 67063 Ludwigshafen. Erfinder: Uwe Dingerdissen ; Günter Lauth ; Andreas Henne ... Y1 - 1998 N1 - Volltext über Datenbank: http://publikationen.dpma.de/ PB - Europäisches Patentamt CY - [München u.a.] ER - TY - BOOK A1 - Lauth, Jakob A1 - Trübenbach, Peter A1 - Hagemeyer, Alfred T1 - Monomodale und polymodale Katalysatorträger und Katalysatoren mit engen Porengrößenverteilungen und deren Herstellverfahren = Monomodal and polymodal catalyst supports and catalysts with a narrow pore size distribution and method for producing the same ; Europäische Patentschrift EP0761307B1 ; Veröffentlichungsdatum 05.02.2003 / Anmelder: BASF Aktiengesellschaft. Erfinder: Peter Trübenbach ; Alfred Hagemeyer ; Günther Lauth ... Y1 - 2003 N1 - Volltext über Datenbank http://publikationen.dpma.de/ ; Falsche Namenansersetzung in Datenbank: Günther PB - Europäisches Patentamt CY - [München u.a.] ER - TY - JOUR A1 - Biselli, Manfred A1 - Kragl, U. A1 - Wandrey, C. T1 - Reaction Engineering for Enzyme-Catalyzed Biotransformations JF - Enzyme catalysis in organic synthesis : a comprehensive handbook. Bd. 1. / ed. by Karlheinz Drauz. Y1 - 1995 SN - 3-527-28479-6 N1 - erschienen: Vol. 1-2 SP - 89 EP - 155 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Biselli, Manfred A1 - Van der Pol, Jens J. A1 - Joksch, Burkhard A1 - Galgens, Jochem T1 - On-line control of an immobilized hybridoma culture with multi-channel flow injektion analysis / Jens J. van der Pol, Burkhard Joksch, Jochem Galgens, Manfred Biselli, Cornelis D. de Gooijer, Johannes Tramper and Christian Wandrey JF - Journal of Biotechnology. 43 (1995), H. 3 Y1 - 1995 SN - 0168-1656 N1 - 3. Autor vermutlich: Jochen Gätgens SP - 229 EP - 242 ER - TY - JOUR A1 - Biselli, Manfred A1 - Thömmes, J. A1 - Born, C. A1 - Wandrey, C. T1 - Purification of monoclonal antibodies by fluidized bed adsorption / Thömmes, J. ; Born, C. Biselli, M. ; Wandrey, C. ; Kula, M.-R. JF - Animal cell technology : developments towards the 21st century / edited by E. C. Beuvery Y1 - 1995 SN - 0-7923-3736-0 N1 - Meeting "Animal Cell Technology, Developments towards the 21st Century" ; <1994.09.12-16 : ; Veldhoven> SP - 515 EP - 519 PB - Kluwer Acad. Press CY - Dordrecht ER - TY - JOUR A1 - Biselli, Manfred A1 - Born, C. A1 - Wandrey, C. T1 - Production of monoclonal antibodies in a pilot scale fluidized bed bioreactor / Born, C.; Biselli, M.; Wandrey, C. JF - Animal cell technology : developments towards the 21st century / edited by E. C. Beuvery Y1 - 1995 SN - 0-7923-3736-0 N1 - Meeting "Animal Cell Technology, Developments towards the 21st Century" ; <1994.09.12-16 : ; Veldhoven> SP - 515 EP - 519 PB - Kluwer Acad. Press CY - Dordrecht ER - TY - JOUR A1 - Biselli, Manfred A1 - Noll, T. A1 - Wandrey, C. T1 - Wirbelschichtreaktor und Biomasse-Monitor : ein leistungsfähiges Fermenationssystem für Säugerzellen / Noll, T. ; Biselli, M. ; Wandrey, C. JF - Biospektrum. 2 (1996), H. 1 Y1 - 1996 SN - 0947-0867 SP - 65 EP - 67 ER - TY - JOUR A1 - Biselli, Manfred A1 - Machnik, M. A1 - Wandrey, C. T1 - Online-Immunoanalytik bei der kontinuierlichen Fermentation von Hybridomazellen / Machnik, M. ; Biselli, M. ; Wandrey, C. JF - BIO WORLD. 1 (1996), H. 3 Y1 - 1996 SN - 1424-8514 SP - 12 EP - 15 ER - TY - JOUR A1 - Biselli, Manfred A1 - Born, C. A1 - Thömmes, J. A1 - Wandrey, C. T1 - An approach to integrated antibody production: Coupling of fluidized bed cultivation and fluidized bed adsorption / Born, C. ; Thömmes, J. ; Biselli, M. ; Wandrey, C. ; Kula, M.-R. JF - Bioprocess Engineering. 15 (1996), H. 1 Y1 - 1996 SN - 1615-7591 SP - 21 EP - 29 ER - TY - JOUR A1 - Biselli, Manfred A1 - Noll, T. A1 - Wandrey, C. T1 - On-line biomass monitoring of immobilized hybridoma cells by dielectrical measurements / Noll, T. ; Biselli, M. ; Wandrey, C. JF - Animal cell technology : from vaccines to genetic medicine ; [Vilamoura, Portugal, May 1996] / ed. by Manuel J. T. Carrondo Y1 - 1997 SN - 0-7923-4321-2 SP - 289 EP - 294 PB - Kluwer CY - Dordrecht ER - TY - JOUR A1 - Biselli, Manfred A1 - Schröder, B. A1 - Herfurth, C. A1 - Meißner, P. T1 - Differentiation of bone marrow cells immobilized on microcarriers in a fluidized bed reactor / Schröder, B. ; Herfurth, C. ; Meißner, P. ; Biselli, M. ; Link, H. ; Wandrey, C. JF - Animal cell technology : from vaccines to genetic medicine ; [Vilamoura, Portugal, May 1996] / ed. by Manuel J. T. Carrondo Y1 - 1997 SN - 0-7923-4321-2 SP - 589 EP - 593 PB - Kluwer CY - Dordrecht ER - TY - JOUR A1 - Biselli, Manfred A1 - Van der Pol, Jens J. A1 - De Gooijer, Cornelis D. A1 - Wandrey, Christian T1 - Automation of selective assays for on-line bioprocess monitoring by flow-injection analysis / van der Pol, Jens J. ; de Gooijer, Cornelis D. ; Biselli, Manfred ; Wandrey, Christian ; Tramper, Johannes JF - Trends in Biotechnology. 14 (1996), H. 12 Y1 - 1996 SN - 0167-7799 SP - 471 EP - 477 ER - TY - BOOK A1 - Mang, Thomas A1 - Heil, Günter A1 - Gartzen, Johannes T1 - Verfahren zur Behandlung von Fotopapier / Patentschrift DE 4105368 C1 Y1 - 1992 N1 - DE 4105368 C1 ; Veröffentlichungstag der Patenterteilung 07.05.1992 ; Volltext recherchierbar über PB - Deutsches Patentamt CY - München ER - TY - JOUR A1 - Baumann, Marcus A1 - Goeyens, L. A1 - Semeneh, M. A1 - Elskens, M. T1 - Phytoplankton nutrient utilisation and nutrient signature in the Southern Ocean / Goeyens, L. ; Semeneh, M. ; Baumann, M.E.M. ; Elskens, M. ; Shopova, D. ; Dehairs, F. JF - Journal of Marine Systems. 17 (1998), H. 1-4 Y1 - 1998 SN - 0924-7963 SP - 143 EP - 157 ER - TY - JOUR A1 - Baumann, Marcus A1 - Semeneh, M. A1 - Dehairs, F. A1 - Elskens, M. T1 - Nitrogen uptake regime and phytoplankton community structure in the Southern Ocean / Semeneh, M. ; Dehairs, F. ; Elskens, M. ; Baumann, M.E.M. ; Kopczynska, E.E. ; Lancelot, C. ; Goeyens, L. JF - Journal of Marine Systems. 17 (1998), H. 1-4 Y1 - 1998 SN - 0924-7963 SP - 159 EP - 177 ER - TY - BOOK A1 - Lauth, Jakob A1 - Dingerdissen, Uwe A1 - Steuerle, Ulrich T1 - Cup catalyst and process for producing aziridines : [Internationale Pantentanmeldung WO9633018] ; Veröffentlichungsdatum: 1996-10-24 / Anmelder: BASF AG ; Dingerdissen, Uwe ; Lauth, Guenther ; Steuerle, Ulrich. Erfinder: Dingerdissen, Uwe ; Lauth, Guenther ; Steuerle, Ulrich Y1 - 1996 PB - [Weltorganisation für geistiges Eigentum] CY - [Genf] ER - TY - BOOK A1 - Lauth, Jakob A1 - Goetz, Norbert A1 - Harreus, Albrecht T1 - Eljárás O-(2-hidroxi-alkil)-oximok elõállítására : [Ungarische Patentanmeldung ; Veröffentlichungsnummer HU216742 ; Veröffentlichungsdatum: 1999-08-30] / Anmelder: BASF AG. Erfinder: Gehrer Eugen ; Goetz Norbert ; Harreus Albrecht ... Guenther Lauth ... Y1 - 1999 N1 - Aktenzeichen HU19970000313 ; Nachweis unter http://hu.espacenet.com/ PB - Gehrer, Eugen CY - [Budapest] ER - TY - PAT A1 - Lauth, Jakob A1 - Gehrer, Eugen A1 - Goetz, Norbert A1 - Harreus, Albrecht T1 - Eljárás O-(2-hidroxi-alkil)-oximok elõállítására : [Ungarische Patentanmeldung ; Veröffentlichungsnummer HU77753 ; Veröffentlichungsdatum: 1998-07-28] / Anmelder: BASF AG. Erfinder: Gehrer Eugen ; Goetz Norbert ; Harreus Albrecht ... Guenther Lauth ... Y1 - 1998 N1 - Aktenzeichen HU9700313A 19950728 ; Nachweis unter http://hu.espacenet.com/ PB - [Hungarian Patent Office] CY - [Budapest] ER - TY - BOOK A1 - Lauth, Jakob A1 - Rang, Harald A1 - Hartmann, Horst A1 - Götz, Norbert T1 - PROCESSO PARA A PREPARAÇÃO DE OXIMAS DE O- (2-HIDROXIALQUILA) : Nº do Pedido: PI9508498-3 ; Data do Depósito: 28/07/1995 / Nome do Titular: Basf Aktiengesellschaft. Nome do Inventor: HARALD RANG ; HORST HARTMANN ; NORBERT GOTZ ... GUNTER LAUTH ... Y1 - 1995 N1 - PCT Número: EP9503001 ; BR000009508498A PB - Instituto Nacional da Propriedade Industrial CY - Rio de Janeiro/RJ ER - TY - PAT A1 - Lauth, Jakob T1 - Preparation of O-(2-hydroxyalkyl) oximes : patent application CA 2196452 ; filing date: 1995-07-28 ; national entry: 1997-01-30 / applicants: BASF Aktiengesellschaft. Inventors: Harreus, Albrecht ; Gotz, Norbert ; Rang, Harald ... Lauth, Guenther ... Y1 - 1995 N1 - International publication number: WO1996/004238 PB - Canadian International Property Office CY - [Gatineau, Quebec] ER - TY - PAT A1 - Lauth, Jakob A1 - Harreus, Albrecht A1 - Goetz, Norbert A1 - Rang, Harald T1 - Preparation of O-(2-hydroxyalkyl) oximes : [South African Patent ] publication number: ZA9506402 ; publication date: 1997-02-03 / applicant: BASF AG. Inventor: Harreus, Albrecht ; Goetz, Norbert ; Rang, Harald .... Lauth, Guenter ... Y1 - 1997 N1 - Also published as WO9604238 PB - [South African Companies and Intellectual Property Registration Office] CY - [Pretoria] ER - TY - BOOK A1 - Lauth, Jakob A1 - Wolf, Gerhard A1 - Burkhart, Bernd T1 - Verfahren zur Herstellung von Alkoxylierungsprodukten in Gegenwart von mit Additiven modifizierten Mischhydroxiden : [Österreichische Patentschrift] AT172707T ; Veröffentlichungsdatum: 1998-11-15 / Anmelder: BASF AG. Erfinder: Gerhard Wolf ; Bernd Burghart ; Günter Lauth ... Y1 - 1998 N1 - U.a. auch veröffentlicht als WO9504024. PB - [Österreichisches Patentamt] CY - [Wien] ER - TY - PAT A1 - Lauth, Jakob A1 - Müller, Ulrich A1 - Hoelderich, Wolfgang T1 - Colored crystalline aluminophosphates and/or silicoaluminophosphates of the AEL or VFI type : United States Patent ; patent number 5,360,474 ; date of patent: Nov. 1, 1994 / assignee: BASF Aktiengesellschaft Y1 - 1994 N1 - Barcode: US005360474A ; Volltext über Datenbank: http://www.google.com/patents PB - [United States Trademark and Patent Office] CY - [Alexandria, VA] ER - TY - PAT A1 - Lauth, Jakob A1 - Wolf, Gerhard A1 - Burkhart, Bernd T1 - Preparation of alkoxylation products in the presence of mixed hydroxides modified with additives : United States Patent ; patent number 5,741,947 ; date of patent: Apr. 21, 1998 / assignee: BASF Aktiengesellschaft. Inventors: Gerhard Wolf ; Bernd Burkhart ; Guenter Lauth ... Y1 - 1998 N1 - Barcode: US005741947A ; Volltext über Datenbank: http://www.google.com/patents PB - [United States Trademark and Patent Office] CY - [Alexandria, VA] ER - TY - PAT A1 - Lauth, Jakob A1 - Hoelderich, Wolfgang A1 - Wagenblast, Gerhard T1 - Molecular sieves containing azo dyes : United States Patent ; patent number 5,573,585 ; date of patent: Nov. 12, 1996 / assignee: Wolfgang Hoelderich. Inventors: Guenter Lauth ; Wolfgang Hoelderich ; Gerhard Wagenblast ... Y1 - 1996 N1 - Barcode: US005573585A ; Volltext über Datenbank: http://www.google.com/patents PB - [United States Trademark and Patent Office] CY - [Alexandria, VA] ER - TY - PAT A1 - Lauth, Jakob A1 - Dingerdissen, Uwe A1 - Henne, Andreas T1 - Preparation of amines : United States Patent ; patent number 5,817,871 ; date of patent: Oct. 6, 1998 / Assignee: BASF Aktiengesellschaft. Inventors: Uwe Dingerdissen ; Gunter Lauth ; Andreas Henne .... Y1 - 1998 N1 - Barcode: US005817871A ; Volltext über Datenbank: http://www.google.com/patents ; Falsche Namensansetzung: Gunter ... PB - [United States Trademark and Patent Office] CY - [Alexandria, VA] ER - TY - PAT A1 - Lauth, Jakob A1 - Dingerdissen, Uwe A1 - Trübenbach, Peter A1 - Steuerle, Ulrich T1 - Preparation of aziridines : United States Patent ; patent number 5,693,825 ; date of patent: Dec. 2, 1997 / assignee: BASF Aktiengesellschaft. Inventors: Uwe Dingerdissen ; Gunter Lauth ; Peter Trubenbach ; Ulrich Steuerle Y1 - 1997 N1 - Barcode: US005324493A ; Volltext über Datenbank: http://www.google.com/patents ; Falsche Namensansetzung in Datenbank: Gunter ... PB - United States Patent and Trademark Office CY - [Alexandria, VA] ER - TY - PAT A1 - Lauth, Jakob A1 - Trübenbach, Peter A1 - Hagemeyer, Alfred T1 - Monomodal and polymodal catalyst supports and catalysts having narrow pore size distributions and their production : United States Patent ; patent number 5,935,898 ; date of patent: Aug. 10, 1999 / assignee: BASF Aktiengesellschaft. Inventors: Peter Trubenbach ; Alfred Hagemeyer ; Gunter Lauth ... Y1 - 1999 N1 - Barcode: US005935898A ; Volltext über Datenbank: http://www.google.com/patents ; Falsche Namensansetzung in Datenbank: Gunter ... PB - [United States Patent and Trademark Office] CY - [Alexandria, VA] ER - TY - JOUR A1 - Baumann, Marcus A1 - Jahnke, J. T1 - Die marine Planktonalge Phaeocystis globosa / Jahnke, J. ; Baumann, M. JF - Mikrokosmos. 75 (1986), H. 12 Y1 - 1986 SN - 0026-3680 SP - 357 EP - 359 ER - TY - JOUR A1 - Baumann, Marcus A1 - Tillmann, U. A1 - Aletsee, L. T1 - Distribution of Carbon Among Photosynthetic End Products in the Bloom-Forming Arctic Diatom Thalassiosira antarctica COMBER / Tillmann, U. ; Baumann, M.E.M. ; Aletsee, L. JF - Polar Biology. 10 (1989), H. 3 Y1 - 1989 SN - 0722-4060 SP - 231 EP - 238 ER - TY - JOUR A1 - Baumann, Marcus A1 - Gradinger, R. T1 - Distribution of Phytoplankton Communities in Relation to the Large Scale Hydrographical Regime in the Fram Strait 1984 / Gradinger, R. ; Baumann, M.E.M. JF - Marine Biology. 111 (1991), H. 2 Y1 - 1991 SN - 0025-3162 SP - 311 EP - 321 ER - TY - JOUR A1 - Baumann, Marcus A1 - Hirche, H.-J. A1 - Kattner, G. A1 - Gradinger, R. T1 - Plankton distribution and the impact of copepod grazing on primary production in Fram Strait, Greenland Sea / Hirche, H.-J. ; Baumann, M.E.M. ; Kattner, G.; Gradinger, R. JF - Journal of Marine Systems. 2 (1991), H. 3-4 Y1 - 1991 SN - 0924-7963 SP - 477 EP - 494 ER - TY - JOUR A1 - Baumann, Marcus A1 - Thomas, D. A1 - Gleitz, M. T1 - Efficiency of carbon assimilation and photoacclimation in a small unicellular Chaetoceros species from the Weddel Sea (Antarctica): Influence of temperature and irridiance / Thomas, D. ; Baumann, M.E.M. ; Gleitz, M. JF - Journal of Experimental Marine Biology and Ecology. 157 (1992), H. 2 Y1 - 1992 SN - 0022-0981 SP - 195 EP - 209 ER - TY - JOUR A1 - Baumann, Marcus A1 - Brandini, Frederico A1 - Staubes, Regina T1 - The influence of light and temperature on carbonspecific DMS release by cultures of Phaeocystis antarctica and three antarctic diatoms / Baumann, Marcus E.M. ; Brandini, Frederico ; Staubes, Regina JF - Marine Chemistry. 45 (1994), H. 1-2 Y1 - 1994 SN - 0304-4203 SP - 129 EP - 136 ER - TY - JOUR A1 - Baumann, Marcus A1 - Lancelot, C. A1 - Brandini, F. A1 - Sakshaug, E. T1 - The taxonomic identity of the cosmopolitan prymnesiophyte Phaeocystis, a morphological and ecophysiological approach / Baumann, M.E.M. ; Lancelot, C. ; Brandini, F. ; Sakshaug, E. ; John M. JF - Journal of Marine Systems. 5 (1994), H. 1 Y1 - 1994 SN - 0924-7963 SP - 5 EP - 22 ER - TY - JOUR A1 - Baumann, Marcus A1 - Rousseau, V. A1 - Vaulot, D. A1 - Casotti, R. T1 - The life cycle of Phaeocystis (Prymnesiophycae): evidence and hypotheses / Rousseau, V. ; Vaulot, D. ; Casotti, R. ; Cariou, V. ; Lenz, J. ; Gunkel, J. ; Baumann, M. JF - Journal of Marine Systems. 5 (1994), H. 1 Y1 - 1994 SN - 0924-7963 SP - 23 EP - 39 ER - TY - JOUR A1 - Baumann, Marcus A1 - Goeyens, L. A1 - Tréguer, P. A1 - Baeyens, W. T1 - The leading role of ammonium in the nitrogen uptake regime of Southern Ocean marginal ice zones / Goeyens, L. ; Tréguer, P. ; Baumann, M.E.M. ; Baeyens, W. ; Dehairs, F. JF - Journal of Marine Systems. 6 (1995), H. 4 Y1 - 1995 SN - 0924-7963 SP - 345 EP - 361 ER - TY - JOUR A1 - Baumann, Marcus A1 - Lara, R.J. A1 - Hubberten, D.N. A1 - Thomas, D.N. T1 - Dissolved organic matter studies in enclosed systems: Application of hydophobic fractionation for the assessment of organic nitrogen dynamics / Lara, R.J. ; Hubberten, U. ; Thomas, D.N. ; Baumann, M.E.M. ; Kattner, G. JF - Journal of Marine Systems. 13 (1997), H. 1-4 Y1 - 1997 SN - 0924-7963 SP - 155 EP - 161 ER - TY - JOUR A1 - Baumann, Marcus A1 - Jahnke, J. T1 - Chemical and Physical Effects on the Shape and Growth of the Diatom Biddulphia sinensis GREVILLE in Batch Cultures: A Contribution to Bioindication in Plankton Ecology / Jahnke, J. ; Baumann, M. JF - Hydrobiological Bulletin . 17 (1983), H. 1 Y1 - 1983 SN - 0165-1404 SP - 5 EP - 20 ER - TY - JOUR A1 - Baumann, Marcus A1 - Jahnke, J. T1 - Marine Planktonalgen der Arktis I. Die Haptophycee Phaeocystis pouchetii / Baumann, M. ; Jahnke, J. JF - Mikrokosmos. 75 (1986), H. 9 Y1 - 1986 SN - 0026-3680 SP - 262 EP - 265 ER - TY - JOUR A1 - Baumann, Marcus A1 - Krämer, M. T1 - Marine Planktonalgen der Arktis II. Diatomeen / Baumann, M. ; Krämer, M. JF - Mikrokosmos. 75 (1986), H. 10 Y1 - 1986 SN - 0026-3680 SP - 306 EP - 310 ER - TY - PAT A1 - Lauth, Jakob A1 - Hoelderich, Wolfgang A1 - Harth, Klaus A1 - Hibst, Hartmuth T1 - Coated catalysts : United States Patent ; patent number 5,559,065 ; date of patent: Sep. 24, 1996 / assignee: BASF Aktiengesellschaft Y1 - 1996 N1 - Barcode: US005559065A ; Volltext über Datenbank: http://www.google.com/patents PB - [United States Trademark and Patent Office] CY - [Alexandria, VA] ER - TY - PAT A1 - Lauth, Jakob A1 - Müller, Ulrich A1 - Hoelderich, Wolfgang T1 - Preparation of aluminophosphates and silicoaluminophosphates having the AEL structure using 1,2-bis-( 4-pyridyl)-ethane : United States Patent ; patent number 5,324,493 ; date of patent: Jun. 28, 1994 / assignee: BASF Aktiengesellschaft. Inventors: Ulrich Mueller ; Wolfgang Hoelderich ; Guenter Lauth Y1 - 1994 N1 - Barcode: US005324493A ; Volltext über Datenbank: http://www.google.com/patents PB - [United States Trademark and Patent Office] CY - [Alexandria, VA] ER - TY - JOUR A1 - Wagner, Torsten A1 - Molina, R. A1 - Biselli, Manfred A1 - Canzoneri, Michele A1 - Schnitzler, Thomas A1 - Yoshinobu, Tatsuo A1 - Schöning, Michael Josef T1 - A light-addressable potentiometric sensor system for fast, simultaneous and spatial detection of the metabolic activity of biological cells JF - Transducers '07 Eurosensors XXI : digest of technical papers ; the14th International Conference on Solid-State Sensors, Actuators and Microsystems, June 10-14, 2007, Lyon, France / Gilles Delapierre (Ed.) Y1 - 2007 SN - 1-4244-0841-5 N1 - Eurosensors 21, 2007, Lyon ; International Conference on Solid-State Sensors, Actuators and Microsystems 14, 2007, Lyon SP - 1107 EP - 1110 PB - IEEE CY - Piscataway ER - TY - JOUR A1 - Selmer, Thorsten A1 - Jennemann, Richard A1 - Bauer, Bernhard L. A1 - Bertalanffy, Helmut T1 - Novel glycoinositolphosphosphingolipids, basidiolipids, from Agaricus / Jennemann, Richard ; Bauer, Bernhard, L. ; Bertalanffy, Helmut ; Geyer, Rudolf ; Gschwind, Ruth, M. ; Selmer, Thorsten ; Wiegandt, Herbert JF - European Journal of Biochemistry. 259 (1999), H. 1-2 Y1 - 1999 SN - 0014-2956 SP - 331 EP - 338 ER - TY - JOUR A1 - Selmer, Thorsten A1 - Hallmann, Armin A1 - Schmidt, Bernhard A1 - Sumper, Manfred T1 - The Evolutionary Conservation of a Novel Protein Modification, the Conversion of Cysteine to Serinesemialdehyde in Arylsulfatase from Volvox carteri / Selmer, Thorsten ; Hallmann, Armin ; Schmidt, Bernhard ; Sumper, Manfred ; Figura, Kurt von JF - European Journal of Biochemistry. 238 (1996), H. 2 Y1 - 1996 SN - 0014-2956 SP - 341 EP - 345 ER - TY - JOUR A1 - Selmer, Thorsten A1 - Scott, Richard A1 - Näser, Ulrike A1 - Friedrich, Peter T1 - Stereochemistry of hydrogen removal from the 'unactivated' C-3 position of 4-hydroxybutyryl-CoA catalysed by 4-hydroxybutyryl-CoA dehydratase / Scott, R. ; Näser, U. ; Friedrich, P. ; Selmer, T. ; Buckel, W. ; Golding, BT. JF - Chemical Communications : ChemCom (2004) Y1 - 2004 SN - 1364-548X SP - 1210 EP - 1211 ER - TY - JOUR A1 - Selmer, Thorsten A1 - Thamer, Wiebke A1 - Cirpus, Irina A1 - Hans, Marcus T1 - A two [4Fe-4S]-cluster-containing ferredoxin as an alternative electron donor for 2-hydroxyglutaryl-CoA dehydratase from Acidaminococcus fermentans / Thamer, Wiebke ; Cirpus, Irina ; Hans, Marcus ; Pierik, Antonio, J. ; Selmer, Thorsten ; Bill, Eckhard ; JF - Archives of Microbiology. 179 (2003), H. 3 Y1 - 2003 SN - 1432-072X SP - 197 EP - 204 ER - TY - JOUR A1 - Selmer, Thorsten A1 - Netz, Daili Jacqueline Aguilar A1 - Pohl, Regula A1 - Beck-Sickinger, Annette G. T1 - Biochemical characterisation and genetic analysis of aureocin A53, a new, atypical bacteriocin from Staphylococcus aureus / Netz, Daili Jacqueline Aguilar ; Pohl, Regula ; Beck-Sickinger, Annette G. ; Selmer, Thorsten ; Pierik, Antonio J. ; Carmo de Frei JF - Journal of Molecular Biology. 319 (2002), H. 3 Y1 - 2002 SN - 0022-2836 SP - 745 EP - 756 ER - TY - JOUR A1 - Selmer, Thorsten A1 - Schmidt, Bernhard A1 - Ingendoh, Arnd A1 - Figura, Kurt von T1 - A novel amino acid modification in sulfatases that is defective in multiple sulfatase deficiency / Schmidt, Bernhard ; Selmer, Thorsten ; Ingendoh, Arnd ; Figurat, Kurt von JF - Cell. 82 (1995), H. 2 Y1 - 1995 SN - 0092-8674 SP - 271 EP - 278 ER - TY - JOUR A1 - Selmer, Thorsten A1 - Kahnt, Jörg A1 - Goubeaud, Marcel A1 - Shima, Seigo T1 - The biosynthesis of methylated amino acids in the active site region of methyl-coenzyme M reductase / Selmer, Thorsten ; Kahnt, Jörg ; Goubeaud, Marcel ; Shima, Seigo ; Grabarse, Wolfgang ; Ermler, Ulrich ; Thauer, Rudolf K. JF - Journal of Biological Chemistry. 275 (2000), H. 6 Y1 - 2000 SN - 1083-351X SP - 3775 EP - 3760 ER - TY - JOUR A1 - Selmer, Thorsten A1 - Brüser, Thomas A1 - Dahl, Christiane T1 - ADP Sulfurylase" from Thiobacillus denitrificans Is an Adenylylsulfate:Phosphate Adenylyltransferase and Belongs to a New Family of Nucleotidyltransferases / Brüser, Thomas ; Selmer, Thorsten ; Dahl, Christiane JF - Journal of Biological Chemistry. 275 (2000), H. 3 Y1 - 2000 SN - 1083-351X SP - 1691 EP - 1690 ER - TY - JOUR A1 - Selmer, Thorsten A1 - Buckel, Wolfgang T1 - Oxygen Exchange between Acetate and the Catalytic Glutamate Residue in Glutaconate CoA-transferase from Acidaminococcus fermentans. IMPLICATIONS FOR THE MECHANISM OF CoA-ESTER HYDROLYSIS JF - Journal of Biological Chemistry. 274 (1999), H. 30 Y1 - 1999 SN - 1083-351X SP - 20772 EP - 20778 ER - TY - JOUR A1 - Selmer, Thorsten A1 - Recksiek, Michael A1 - Dierks, Thomas A1 - Schmidt, Bernhard T1 - Sulfatases, Trapping of the Sulfated Enzyme Intermediate by Substituting the Active Site Formylglycine / Recksiek, Michael ; Selmer, Thorsten ; Dierks, Thomas ; Schmidt, Bernhard ; Figura, Kurt von JF - Journal of Biological Chemistry. 273 (1998), H. 11 Y1 - 1998 SN - 1083-351X SP - 6096 EP - 6103 ER - TY - JOUR A1 - Selmer, Thorsten A1 - Miech, Claudia A1 - Dierks, Thomas A1 - Figura, Kurt von T1 - Arylsulfatase from Klebsiella pneumoniae Carries a Formylglycine Generated from a Serine / Miech, Claudia ; Dierks, Thomas ; Selmer, Thorsten ; Figura, Kurt von ; Schmidt, Bernd JF - Journal of Biological Chemistry. 273 (1998), H. 9 Y1 - 1998 SN - 1083-351X SP - 4835 EP - 4837 ER - TY - JOUR A1 - Selmer, Thorsten A1 - Sommerlade, Hans-Jörg A1 - Ingendoh, Arnd A1 - Gieselmann, Volkmar T1 - Glycosylation and phosphorylation of arylsulfatase A / Sommerlade, Hans-Jörg. ; Selmer, Thomas. ; Ingendoh, Arnd ; Gieselmann, Volkmar ; Figura, Kurt von ; Neifer, Klaus ; Schmidt, Bernhard JF - Journal of Biological Chemistry. 269 (1994), H. 33 Y1 - 1994 SN - 1083-351X SP - 20977 EP - 20981 ER - TY - JOUR A1 - Selmer, Thorsten A1 - Jennemann, Richard A1 - Bauer, Bernhard L. A1 - Bertalanffy, Helmut T1 - Basidiolipids from Agaricus are novel immune adjuvants / Jennemann, R. ; Bauer, BL. ; Bertalanffy, H. ; Selmer, T. ; Wiegandt, H. JF - Immunobiology. 200 (1999), H. 2 Y1 - 1999 SN - 0171-2985 N1 - PMID: 10416134 SP - 277 EP - 289 ER - TY - JOUR A1 - Selmer, Thorsten A1 - Figura, Kurt von A1 - Schmidt, Bernhard A1 - Dierks, T. T1 - A novel protein modification generating an aldehyde group in sulfatases: its role in catalysis and disease / Figura, Kurt von ; Schmidt, Bernhard ; Selmer, Thorsten ; Dierks, Thomas JF - Bioessays. 20 (1998), H. 6 Y1 - 1998 SN - 1521-1878 SP - 505 EP - 510 ER - TY - JOUR A1 - Selmer, Thorsten A1 - Lukatela, G. A1 - Krauss, N. A1 - Theis, K. T1 - Crystal structure of human arylsulfatase A: the aldehyde function and the metal ion at the active site suggest a novel mechanism for sulfate ester hydrolysis / Lukatela, G. ; Krauss, N. ; Theis, K. ; Selmer, T. ; Gieselmann, V. ; Figura, K. von ; Saenger, JF - Biochemistry. 37 (1998), H. 11 Y1 - 1998 SP - 3654 EP - 3664 ER - TY - JOUR A1 - Selmer, Thorsten A1 - Kim, Jihoe A1 - Darley, Daniel A1 - Buckel, Wolfgang T1 - Characterization of (R)-2-hydroxyisocaproate dehydrogenase and a family III coenzyme A transferase involved in reduction of L-leucine to isocaproate by Clostridium difficile / Kim, J. ; Darley, D. ; Selmer, T. ; Buckel, W. JF - Applied and Environmental Microbiology. 72 (2006), H. 9 Y1 - 2006 SN - 0099-2240 N1 - PMID: 16957230 SP - 6062 EP - 6069 ER - TY - JOUR A1 - Selmer, Thorsten A1 - Yu, Lihua A1 - Blaser, Martin A1 - Andrei, Paula I. T1 - 4-Hydroxyphenylacetate decarboxylases: properties of a novel subclass of glycyl radical enzyme systems / Yu, L. ; Blaser, M. ; Andrei, PI. ; Pierik, AJ. Selmer, T. JF - Biochemistry. 31 (2006), H. 45 Y1 - 2006 N1 - PMID: 16878993 SP - 9584 EP - 9592 ER - TY - JOUR A1 - Selmer, Thorsten A1 - Pierik, Antonio J. A1 - Heider, Johann T1 - New glycyl radical enzymes catalysing key metabolic steps in anaerobic bacteria JF - Biological Chemistry. 386 (2005), H. 10 Y1 - 2005 SN - 1431-6730 SP - 981 EP - 988 ER - TY - JOUR A1 - Selmer, Thorsten A1 - Achebach, Stephanie A1 - Unden, Gottfried T1 - Properties and significance of apoFNR as a second form of air-inactivated [4Fe-4S]·FNR of Escherichia coli / Achebach, S. ; Selmer, T. ; Unden, G. JF - The FEBS Journal. 272 (2005), H. 16 Y1 - 2005 SN - 1742-464X SP - 4260 EP - 4269 ER - TY - JOUR A1 - Selmer, Thorsten A1 - Hermann, Gloria A1 - Jessen, Holly A1 - Gokarn, Ravi R. T1 - Two beta-alanyl-CoA:ammonia lyases in Clostridium propionicum / Herrmann , G. ; Selmer, T. ; Jessen, HJ. ; Gokarn, RR. ; Selifonova, O. ; Gort , SJ. ; , Buckel, W. JF - The FEBS Journal. 272 (2005), H. 3 Y1 - 2005 SN - 1742-464X SP - 813 EP - 821 ER - TY - JOUR A1 - Selmer, Thorsten A1 - Andrei, Paula I. A1 - Pierik, Antonio J. A1 - Zauner, Stefan T1 - Subunit composition of the glycyl radical enzyme p-hydroxyphenylacetate decarboxylase. A small subunit, HpdC, is essential for catalytic activity / Andrei, PI. ; Pierik, AJ. ; Zauner , S. ; Andrei-Selmer, LC. ; Selmer, T. JF - European Journal of Biochemistry. 271 (2004), H. 11 Y1 - 2004 SN - 0014-2956 SP - 2225 EP - 2230 ER - TY - JOUR A1 - Selmer, Thorsten A1 - Hetzel, Marc A1 - Brock, Matthias A1 - Pierik, Antonio J. T1 - Acryloyl-CoA reductase from Clostridium propionicum. An enzyme complex of propionyl-CoA dehydrogenase and electron-transferring flavoprotein / Hetzel, Marc ; Brock, Matthias ; Selmer, Thorsten, Pierik, Antonio J. ; Golding, Bernard T. ; Buckel, Wolfgang JF - European Journal of Biochemistry. 270 (2003), H. 5 Y1 - 2003 SN - 0014-2956 SP - 902 EP - 910 ER - TY - JOUR A1 - Selmer, Thorsten A1 - Willanzheimer, Angela A1 - Hetzel, Marc T1 - Propionate CoA-transferase from Clostridium propionicum. Cloning of the gene and identification of glutamate 324 at the active site JF - European Journal of Biochemistry. 269 (2002), H. 1 Y1 - 2002 SN - 0014-2956 SP - 372 EP - 380 ER - TY - JOUR A1 - Selmer, Thorsten A1 - Andrei, Paula I. T1 - p-Hydroxyphenylacetate decarboxylase from Clostridium difficile. A novel glycyl radical enzyme catalysing the formation of p-cresol JF - European Journal of Biochemistry. 268 (2001), H. 5 Y1 - 2001 SN - 0014-2956 SP - 1363 EP - 1372 ER - TY - JOUR A1 - Selmer, Thorsten A1 - Darley, Dan J. A1 - Clegg, William A1 - Harrington, Ross W. T1 - Stereocontrolled Synthesis of (2R,3S)-2-Methylisocitrate, a Central Intermediate in the Methylcitrate Cycle / Darley, Dan J. ; Selmer, Thorsten ; Clegg, William ; Harrington, Ross W. ; Buckel, Wolfgang ; Golding, Bernardt JF - Helvetica chimica acta. 86 (2003), H. 12 Y1 - 2003 SN - 1522-2675 SP - 3991 EP - 3999 ER - TY - BOOK A1 - Selmer, Thorsten T1 - Nachweis einer neuartigen posttranslationalen Modifikation in Sulfatasen und ihr Fehlen in Enzymen aus Patienten mit multipler Sulfatase-Defizienz Y1 - 1996 N1 - Zugl.: Göttingen, Univ., Diss., 1996 PB - Cuvillier CY - Göttingen ER - TY - JOUR A1 - Jeromin, Günter Erich A1 - Zoor, Annegreth T1 - A new irreversible enzyme-aided esterification method in organic solvents JF - Biotechnology letters. 30 (2008), H. 5 Y1 - 2008 SN - 1573-6776 SP - 925 EP - 928 ER - TY - JOUR A1 - Elbers, Gereon A1 - Nieren, Monika A1 - Hoffmann, Katharina A1 - Gäb, Martina T1 - Schnellerfassung der Wirkung von Luftschadstoffen auf Pflanzen mit der Nahinfrarotspektroskopie (NIRS). Untersuchung Ammoniak exponierter Gräser und Kräuter / Nieren, Monika ; Hoffmann, Katharina ; Gäb, Martina ; Ooyen, Sven van ; Köllner, Barbara ; Elbe JF - Immissionsschutz. 13 (2008), H. 2 Y1 - 2008 SN - 1430-9262 SP - 82 EP - 90 ER - TY - JOUR A1 - Mang, Thomas A1 - Hodenius, M. A. J. A1 - Schmitz-Rode, T. A1 - Baumann, M. T1 - Absorption of 10-hydroxycamptothecin into the coat of magnetoliposomes / Hodenius, M. A. J. ; Schmitz-Rode, T. ; Baumann, M. ; Ivoanova, G. ; Wong, J. E. ; Mang, T. ; Haulena, F. ; Soenen, S. J. H. ; De Cuyper, M. JF - Colloids and Surfaces A: Physicochemical and Engineering Aspects. 343 (2009), H. 1-3 Y1 - 2009 SN - 0927-7757 N1 - Colloid and Surface Science for Nanotechnology SP - 20 EP - 23 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Mang, Thomas A1 - Kolz, J. A1 - Goga, N. A1 - Casanova, F. T1 - Spatial localization with single-sided NMR sensors / Kolz, J. ; Goga, N. ; Casanova, F. ; Mang, T. ; Blümich, B. JF - Applied Magnetic Resonance. 32 (2007), H. 1-2 Y1 - 2007 SN - 0937-9347 SP - 171 EP - 184 ER - TY - JOUR A1 - Scherer, Ulrich W. A1 - Jacobi, M. A1 - Castillo, J. A1 - Foerstel, D. H. T1 - Ultra-low-level measurements of 3H and 14C in wines and champagne / Scherer, U. W. ; Jacobi, M. ; Castillo, J. ; Foerstel, D. H. JF - Radiation effects and defects in solids. 164 (2009), H. 5-6 Y1 - 2009 SN - 1042-0150 SP - 382 EP - 385 ER - TY - JOUR A1 - Scherer, Ulrich W. A1 - Santana, H. H. S. A1 - Maier, G. A1 - Rodenas, J. T1 - Analysis of mechanical strength in ceramic pellets of nuclear fuel / Santana, H. H. S. ; Maier, G. ; Scherer, U. W. ; Rodenas, J. JF - Radiation effects and defects in solids. 164 (2009), H. 5-6 Y1 - 2009 SN - 1042-0150 SP - 313 EP - 318 PB - Taylor & Francis CY - London ER - TY - JOUR A1 - Balakrishnan, Karthikeyan A1 - Andrei-Selmer, Luminita-Cornelia A1 - Selmer, Thorsten A1 - Bacher, Michael A1 - Dodel, Richard T1 - Comparison of Intravenous Immunoglobulins for Naturally Occurring Autoantibodies against Amyloid-β JF - Journal of Alzheimer's Disease Y1 - 2010 SN - 1387-2877 VL - 20 IS - 1 SP - 135 EP - 143 ER - TY - JOUR A1 - Selmer, Thorsten A1 - Pinkenburg, Olaf T1 - Method of cloning at least one nucleic acid molecule of interest using type IIS restriction endonucleases, and corresponding cloning vectors, kits and system using type IIS restriction endonucleases / Selmer, Thorsten ; Pinkenburg, Olaf Y1 - 2008 N1 - Patent No. WO 2008095927 A1 Aug 14, 2008 ER - TY - JOUR A1 - Elbers, Gereon T1 - Identification of marker peptides in hair for discrimination with regard to species and breed / Koerner, Andrea ; Ring, Maike ; Fabry, Marlies ; Elbers, Gereon ; Moeller, Martin JF - Proceedings of the Aachen-Dresden International Textile Conference, 3rd, Aachen, Germany, Nov. 26-27, 2009 Y1 - 2009 N1 - Aachen-Dresden International Textile Conference ; (3 : ; 2009.11.26-27 : ; Aachen) PB - DWI CY - Aachen ER - TY - JOUR A1 - Werner, Frederik A1 - Groebel, Simone A1 - Schuhmacher, K. A1 - Spelthahn, Heiko A1 - Wagner, Torsten A1 - Selmer, Thorsten A1 - Baumann, Marcus A1 - Schöning, Michael Josef T1 - Bestimmung der metabolischen Aktivität von Mikroorganismen während des Biogasbildungsprozesses JF - 9. Dresdner Sensor-Symposium : Dresden, 07.-09. Dezember 2009 / Gerlach, Gerald ; Hauptmann, Peter [Hrsg.] Y1 - 2009 SN - 978-3-941298-44-6 N1 - Dresdner Sensor-Symposium ; (9, 2009, Dresden) SP - 201 EP - 204 PB - TUDpress CY - Dresden ER - TY - JOUR A1 - Mang, Thomas A1 - Kolz, J. A1 - Hunger, K. A1 - Rath, S. T1 - Investigation of self-diffusion processes in ionic hydrogels with a single-sided sensor / Kolz, J. ; Hunger, K. ; Rath, S. ; Mang, T. ; Blümich, B. JF - Magnetic Resonance Imaging. 25 (2007), H. 4 Y1 - 2007 SN - 0730-725X N1 - Proceedings of the Eighth International Bologna Conference on Magnetic Resonance in Porous Media SP - 566 EP - 567 ER - TY - JOUR A1 - Bäcker, Matthias A1 - Beging, Stefan A1 - Biselli, Manfred A1 - Poghossian, Arshak A1 - Wang, J. A1 - Zang, Werner A1 - Wagner, Patrick A1 - Schöning, Michael Josef T1 - Concept for a solid-state multi-parameter sensor system for cell-culture monitoring JF - Electrochimica Acta. 54 (2009), H. 25 Sp. Iss. SI Y1 - 2009 SN - 0013-4686 SP - 6107 EP - 6112 PB - Elsevier CY - Amsterdam ER - TY - BOOK A1 - Jeromin, Günter Erich T1 - Organische Chemie : Ein praxisbezogenes Lehrbuch. Korr. Nachdr. der 2. Aufl. Y1 - 2008 SN - 3-8171-1732-9 PB - Deutsch CY - Frankfurt am Main ER - TY - PAT A1 - Jeromin, Günter Erich T1 - Immobilisierung von Alkoholdehydrogenasen und deren Coenzyme sowie Verwendung des Immobilisats : Offenlegungsschrift : DE 102008038326 A1 Offenlegungstag: 25.02.2010 Y1 - 2010 PB - Deutsches Patent- und Markenamt CY - München ER - TY - PAT A1 - Jeromin, Günter Erich A1 - Schwarzer, Klemens T1 - Neuer Wärmeträger für Solaranlagen : Offenlegungsschrift : DE 102007016738 A1 ; Offenlegungstag: 09.10.2008 Y1 - 2008 N1 - Volltext über Datenbank DEPATISnet: http://depatisnet.dpma.de/DepatisNet/depatisnet?action=pdf&docid=DE102007016738A1 PB - Deutsches Patent- und Markenamt CY - München ER - TY - PAT A1 - Jeromin, Günter Erich T1 - Denk- und Lernspielzeug : Offenlegungsschrift : DE 102006003433 A1 ; Offenlegungstag: 26.07.2007 Y1 - 2007 N1 - Volltext über Datenbank: http://publikationen.dpma.de/ PB - Deutsches Patent- und Markenamt CY - München ER - TY - JOUR A1 - Jeromin, Günter Erich T1 - Superabsorbed alcohol dehydrogenase—a new catalyst for asymmetric reductions JF - Biotechnology Letters. 31 (2009), H. 11 Y1 - 2009 SN - 0141-5492 SP - 1717 EP - 1721 ER - TY - PAT A1 - Mang, Thomas A1 - Krombholz, Markus A1 - Hoffmüller, Winfried A1 - Burchard, Theodor T1 - Verfahren zur Herstellung von Sicherheitsfolien : Offenlegungschrift DE 102007055112 A1 ; Offenlegungstag 10.07.2008 / Krombholz, Markus ; Mang, Thomas ; Hoffmüller, Winfried ; Burchard, Theodor ; Heim, Manfred Y1 - 2008 N1 - Volltext über Datenbank: http://publikationen.dpma.de/ PB - Deutsches Patent- und Markenamt CY - München ER - TY - JOUR A1 - Kowollik, S. A1 - Schnitzler, Thomas A1 - Biselli, Manfred A1 - Krueger, R. A1 - Zang, Werner A1 - Peuscher, A. A1 - Schillberg, S. A1 - Fischer, R. T1 - Die Rolle des Respirationsquotienten in der Zellkulturfermentation JF - Chemie Ingenieur Technik Y1 - 2010 SN - Chemie Ingenieur Tec N1 - Special Issue: ProcessNet-Jahrestagung 2010 und 28. Jahrestagung der Biotechnologen VL - 82 IS - 9 SP - 1505 EP - 1506 ER - TY - JOUR A1 - Biselli, Manfred A1 - Bäcker, Matthias A1 - Poghossian, Arshak A1 - Schöning, Michael Josef A1 - Schnitzler, Thomas A1 - Zang, Werner A1 - Wagner, P. T1 - Entwicklung eines modularen festkörperbasierten Sensorsystems für die Überwachung von Zellkulturfermenationen JF - Sensoren und Messsysteme 2010 [Elektronische Ressource] : Vorträge der 15. ITG/GMA-Fachtagung vom 18. bis 19. Mai 2010 in Nürnberg / Informationstechnische Gesellschaft im VDE (ITG); VDI/VDE-Gesellschaft Mess- und Automatisierungstechnik (GMA) Y1 - 2010 SN - 978-3-8007-3260-9 N1 - Fachtagung Sensoren und Messsysteme 15, 2010, Nürnberg ; Gesellschaft Mess- und Automatisierungstechnik SP - 688 EP - 691 PB - VDE Verlag CY - Berlin ER - TY - JOUR A1 - Werner, Frederik A1 - Krumbe, Christoph A1 - Schumacher, Katharina A1 - Groebel, Simone A1 - Spelthahn, Heiko A1 - Stellberg, Michael A1 - Wagner, Torsten A1 - Yoshinobu, Tatsuo A1 - Selmer, Thorsten A1 - Keusgen, Michael A1 - Baumann, Marcus A1 - Schöning, Michael Josef T1 - Determination of the extracellular acidification of Escherichia coli by a light-addressable potentiometric sensor JF - Physica status solidi (a) : applications and material science. 208 (2011), H. 6 Y1 - 2011 SN - 1862-6319 SP - 1340 EP - 1344 PB - Wiley CY - Weinheim ER - TY - JOUR A1 - Werner, Frederik A1 - Groebel, Simone A1 - Wagner, Torsten A1 - Yoshinobu, Tatsuo A1 - Selmer, Thorsten A1 - Baumann, Marcus A1 - Schöning, Michael Josef T1 - Überwachung der metabolischen Aktivität von Mikroorganismen zur Kontrolle des biologischen Prozesses im Biogasfermenter JF - Biogas 2011 : Energieträger der Zukunft ; 6. Fachtagung, Fachtagung Braunschweig, 08. und 09. Juni 2011 / VDI Energie und Umwelt Y1 - 2011 SN - 978-3-18-092121-1 N1 - Gesellschaft Energie und Umwelt ; Fachtagung Biogas ; (6 : ; 2011.06.08-09 : ; Braunschweig) ; VDI-Berichte ; 2121 SP - 285 EP - 286 PB - VDI-Verl. CY - Düsseldorf ER - TY - JOUR A1 - Martins, Berta M. A1 - Blaser, Martin A1 - Feliks, Mikolaj A1 - Ullmann, Matthias G. A1 - Buckel, Wolfgang A1 - Selmer, Thorsten T1 - Structural basis for a Kolbe-type decarboxylation catalyzed by a glycyl radical enzyme JF - Journal of the American Chemical Society Y1 - 2011 N1 - Just accepted manuscript SP - 1 EP - 33 PB - ACS Publications CY - Washington, DC ER - TY - JOUR A1 - Schiffels, Johannes A1 - Baumann, Marcus A1 - Selmer, Thorsten T1 - Facile analysis of short-chain fatty acids as 4-nitrophenyl esters in complex anaerobic fermentation samples by high performance liquid chromatography JF - Journal of Chromatography A. 1218 (2011), H. 34 Y1 - 2011 SN - 0021-9673 SP - 5848 EP - 5851 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Claessen, O. A1 - Grefen, Dana A1 - Mang, Thomas A1 - Dikland, H. G. A1 - Dikland, H. G. A1 - Duin, M. van T1 - Helle Fensterprofilmaterialien : Alterungsverhalten auf Basis von peroxidisch vernetztem EPDM JF - Kautschuk, Gummi, Kunststoffe : KGK Y1 - 2010 SN - 0948-3276 VL - 63 IS - 9 SP - 350 EP - 360 ER - TY - JOUR A1 - Srivastava, A. A1 - Singh, V. A1 - Aggarwal, P. A1 - Schneeweiss, F. A1 - Scherer, Ulrich W. A1 - Friedrich, W. T1 - Optical studies of insulating polymers for radiation dose monitoring JF - Indian Journal of Pure & Applied Physics Y1 - 2010 SN - 0019-5596 N1 - Special Issue: SI VL - 48 IS - 11 SP - 782 EP - 786 ER - TY - JOUR A1 - Bäcker, Matthias A1 - Delle, L. A1 - Poghossian, Arshak A1 - Biselli, Manfred A1 - Zang, Werner A1 - Wagner, P. A1 - Schöning, Michael Josef T1 - Electrochemical sensor array for bioprocess monitoring JF - Electrochimica Acta (2011) Y1 - 2011 VL - 56 IS - 26 SP - 9673 EP - 9678 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Aggarwal, Prerna A1 - Singh, Virendra A1 - Singh, Arjun A1 - Scherer, Ulrich W. A1 - Singh, Tejvir A1 - Singla, Madan L. A1 - Srivastava, Alok T1 - Physico-chemical transformations in swift heavy ion modified poly(ethyleneterephthalate) JF - Radiation Physics and Chemistry Y1 - 2011 SN - 0969-806X N1 - In Press, Accepted Manuscript SP - 1 EP - 28 PB - Pergamon Press CY - Oxford ER - TY - JOUR A1 - Öhlschläger, Peter A1 - Michel, Nico A1 - Osen, Wolfram A1 - Freyschmidt, Eva-Jasmin T1 - T cell response to human papillomavirus 16 E7 in mice: comparison of Cr release assay, intracellular IFN-gamma production, ELISPOT and tetramer staining / Michel, Nico ; Öhlschläger, Peter ; Osen, Wolfram ; Freyschmidt, Eva-Jasmin ; Gutöhrlein, Heidrun ; JF - Intervirology. 45 (2002) Y1 - 2002 SN - 1423-0100 SP - 290 EP - 299 ER - TY - JOUR A1 - Öhlschläger, Peter A1 - Osen, Wolfram A1 - Dell, Kerstin A1 - Faath, Stefan T1 - Human papillomavirus type 16 L1 capsomeres induce L1-specific cytotoxic T lymphocytes and tumor regression in C57BL/6 mice / Öhlschläger, Peter ; Osen, Wolfram ; Dell, Kerstin ; Faath, Stefan ; Garcea Robert L: ; Jochmus, Ingrid ; Müller, Martin, Pawlita, JF - Journal of Virology. 77 (2003), H. 8 Y1 - 2003 SN - 1098-5514 SP - 4635 EP - 4645 ER - TY - JOUR A1 - Öhlschläger, Peter A1 - Corvinus, Florian M. A1 - Orth, Carina A1 - Moriggl, Richard T1 - Persistent STAT3 activation in colon cancer is associated with enhanced cell proliferation and tumor growth / Corvinus, Florian, Moriggl, Richard ; Tsareva, Svetlana A. ; Wagner, Stefan ; Pfitzner, Edith B. ; Baus, Daniela ; Kaufmann, Roland : Huber, Luka JF - Neoplasia. 7 (2005), H. 6 Y1 - 2005 SN - 1476-5586 SP - 545 EP - 555 ER - TY - JOUR A1 - Öhlschläger, Peter A1 - Quetting, Michael A1 - Alvarez, Gerardo A1 - Dürst, Matthias A1 - Gissmann, Lutz A1 - Kaufmann, Andreas M. T1 - Enhancement of immunogenicity of a therapeutic cervical cancer DNA-based vaccine by co-application of sequence-optimized genetic adjuvants JF - International Journal of Cancer Y1 - 2009 SN - 1097-0215 VL - 125 IS - 1 SP - 189 EP - 198 PB - Wiley CY - Weinheim ER - TY - JOUR A1 - Oosterhuis, Koen A1 - Öhlschläger, Peter A1 - Berg, Joost H. van den A1 - Toebes, Mireille A1 - Gomez, Raquel A1 - Schumacher, Ton N. A1 - Haanen, John B. T1 - Preclinical development of highly effective and safe DNA vaccines directed against HPV 16 E6 and E7 JF - International Journal of Cancer Y1 - 2011 SN - 1097-0215 VL - 129 IS - 2 SP - 397 EP - 406 PB - Wiley CY - Weinheim ER - TY - JOUR A1 - Öhlschläger, Peter A1 - Spies, Elmar A1 - Alvarez, Gerardo A1 - Quetting, Michael A1 - Groettrup, Marcus T1 - The combination of TLR-9 adjuvantation and electroporation-mediated delivery enhances in vivo antitumor responses after vaccination with HPV-16 E7 encoding DNA JF - International Journal of Cancer. 128 (2011), H. 2 Y1 - 2011 SN - 1097-0215 SP - 473 EP - 481 PB - Wiley CY - Weinheim ER - TY - JOUR A1 - Leurs, Ulrike A1 - Mezo, Gabor A1 - Öhlschläger, Peter A1 - Orban, Erika A1 - Marquard, Andrea A1 - Manea, Marilena T1 - Design, synthesis, in vitro stability and cytostatic effect of multifunctional anticancer drug-bioconjugates containing GnRH-III as a targeting moiety JF - Peptide Science N2 - Bioconjugates containing the GnRH-III hormone decapeptide as a targeting moiety are able to deliver chemotherapeutic agents specifically to cancer cells expressing GnRH receptors, thereby increasing their local efficacy while limiting the peripheral toxicity. However, the number of GnRH receptors on cancer cells is limited and they desensitize under continuous hormone treatment. A possible approach to increase the receptor mediated tumor targeting and consequently the cytostatic effect of the bioconjugates would be the attachment of more than one chemotherapeutic agent to one GnRH-III molecule. Here we report on the design, synthesis and biochemical characterization of multifunctional bioconjugates containing GnRH-III as a targeting moiety and daunorubicin as a chemotherapeutic agent. Two different drug design approaches were pursued. The first one was based on the bifunctional [4Lys]-GnRH-III (Glp-His-Trp-Lys-His-Asp-Trp-Lys-Pro-Gly-NH2) containing two lysine residues in positions 4 and 8, whose ϵ-amino groups were used for the coupling of daunorubicin. In the second drug design, the native GnRH-III (Glp-His-Trp-Ser-His-Asp-Trp-Lys-Pro-Gly-NH2) was used as a scaffold; an additional lysine residue was coupled to the ϵ-amino group of 8Lys in order to generate two free amino groups available for conjugation of daunorubicin. The in vitro stability/degradation of all synthesized compounds was investigated in human serum, as well as in the presence of rat liver lysosomal homogenate. Their cellular uptake was determined on human breast cancer cells and the cytostatic effect was evaluated on human breast, colon and prostate cancer cell lines. Compared with a monofunctional compound, both drug design approaches resulted in multifunctional bioconjugates with increased cytostatic effect. Y1 - 2012 U6 - http://dx.doi.org/10.1002/bip.21640 SN - 1097-0282 VL - 98 IS - 1 SP - 1 EP - 10 PB - Wiley CY - New York, NY ER - TY - JOUR A1 - Manea, Marilena A1 - Leurs, Ulrike A1 - Orban, Erika A1 - Baranyai, Zsuzsa A1 - Öhlschläger, Peter A1 - Marquardt, Andreas A1 - Schulcz, Akos A1 - Tejeda, Miguel T1 - Enhanced Enzymatic Stability and Antitumor Activity of Daunorubicin-GnRH-III Bioconjugates Modified in Position 4 JF - Bioconjugate Chemistry Y1 - 2011 SN - 1520-4812 VL - 22 IS - 7 SP - 1320 EP - 1329 PB - ACS CY - Washington, DC ER - TY - JOUR A1 - Spiess, Elmar A1 - Wilfried, Reichardt A1 - Alvarez, Gerardo A1 - Gottrup, Marcus A1 - Öhlschläger, Peter T1 - An Artificial PAP Gene Breaks Self-tolerance and Promotes Tumor Regression in the TRAMP Model for Prostate Carcinoma JF - Molecular Therapy Y1 - 2011 SN - 1525-0016 VL - 20 IS - 3 SP - 555 EP - 564 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Öhlschläger, Peter A1 - Osen, Wolfram A1 - Peiler, Tanja A1 - Caldeira, Sandra T1 - A DNA vaccine based on a shuffled E7 oncogene of the human papillomavirus type 16 (HPV 16) induces E7-specific cytotoxic T cells but lacks transforming activity / Osen, Wolfram ; Peiler, Tanja ; Öhlschläger, Peter ; Caldeira, Sandra ; Faath, Stefan ; Mich JF - Vaccine. 19 (2001), H. 20 Y1 - 2001 SN - 0264-410X SP - 4276 EP - 4286 ER - TY - JOUR A1 - Öhlschläger, Peter A1 - Steinberg, Thorsten A1 - Sehr, Peter A1 - Osen, Wolfram T1 - Modification of HPV 16 E7 genes: correlation between the level of protein expression and CTL response after immunization of C57BL/6 mice / Steinberg, Thorsten ; Öhlschläger, Peter ; Sehr, Peter ; Osen, Wolfram ; Gissmann, Lutz JF - Vaccine. 23 (2005), H. 9 Y1 - 2005 SN - 0264-410X SP - 1149 EP - 1157 ER - TY - JOUR A1 - Öhlschläger, Peter A1 - Pes, Michaela A1 - Osen, Wolfram A1 - Dürst, Matthias T1 - An improved rearranged Human Papillomavirus Type 16 E7 DNA vaccine candidate (HPV-16 E7SH) induces an E7 wildtype-specific T cell response / Öhlschläger, Peter ; Pes, Michaela ; Osen, Wolfram ; Dürst, Matthias ; Schneider, Achim ; Gissmann, Lutz ; Kaufman JF - Vaccine. 24 (2006), H. 15 Y1 - 2006 SN - 0264-410X SP - 2880 EP - 2893 ER - TY - JOUR A1 - Bäcker, Matthias A1 - Raue, Markus A1 - Schusser, Sebastian A1 - Jeitner, C. A1 - Breuer, L. A1 - Wagner, P. A1 - Poghossian, Arshak A1 - Förster, Arnold A1 - Mang, Thomas A1 - Schöning, Michael Josef T1 - Microfluidic chip with integrated microvalves based on temperature- and pH-responsive hydrogel thin films JF - Physica Status Solidi (a) N2 - Two types of microvalves based on temperature-responsive poly(N-isopropylacrylamide) (PNIPAAm) and pH-responsive poly(sodium acrylate) (PSA) hydrogel films have been developed and tested. The PNIPAAm and PSA hydrogel films were prepared by means of in situ photopolymerization directly inside the fluidic channel of a microfluidic chip fabricated by combining Si and SU-8 technologies. The swelling/shrinking properties and height changes of the PNIPAAm and PSA films inside the fluidic channel were studied at temperatures of deionized water from 14 to 36 °C and different pH values (pH 3–12) of Titrisol buffer, respectively. Additionally, in separate experiments, the lower critical solution temperature (LCST) of the PNIPAAm hydrogel was investigated by means of a differential scanning calorimetry (DSC) and a surface plasmon resonance (SPR) method. Mass-flow measurements have shown the feasibility of the prepared hydrogel films to work as an on-chip integrated temperature- or pH-responsive microvalve capable to switch the flow channel on/off. Y1 - 2012 U6 - http://dx.doi.org/10.1002/pssa.201100763 SN - 1862-6319 VL - 209 IS - 5 SP - 839 EP - 845 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Aggarwal, P. A1 - Dhiman, S. A1 - Kumar, G. A1 - Scherer, Ulrich W. A1 - Singla, M. L. A1 - Srivastava, A. T1 - Optical study of poly(ethyleneterephthalate) modified by different ionizing radiation dose JF - Indian Journal of Pure and Applied Physics N2 - Thin films of poly(ethyleneterephthalate) [PET]were exposed to radiation dose ranging from 10 to 30 kGy by using gamma rays in the range 12.8-177.8 MGy using swift light ions of hydrogen. There was no effect of the radiation dose on the optical behaviour of PET as a result of exposure to radiation dose up to 30 kGy brought about by gamma rays but a significant decrease in the optical band gap values was observed when PET was exposed to swift light ions of hydrogen. The data obtained are discussed in terms of optical studies carried out on PET using swift heavy ions. Y1 - 2012 SN - 0019-5596 VL - 50 IS - 2 SP - 129 EP - 132 ER - TY - JOUR A1 - Werner, Frederik A1 - Groebel, Simone A1 - Krumbe, Christoph A1 - Wagner, Torsten A1 - Selmer, Thorsten A1 - Yoshinobu, Tatsuo A1 - Baumann, Marcus A1 - Schöning, Michael Josef T1 - Nutrient concentration-sensitive microorganism-based biosensor JF - Physica Status Solidi (a) Y1 - 2012 U6 - http://dx.doi.org/10.1002/pssa.201100801 SN - 1862-6319 VL - 209 IS - 5 SP - 900 EP - 904 PB - Wiley-VCH CY - Weinheim ER - TY - CHAP A1 - Feuerriegel, Uwe A1 - Wittenhorst, Simon A1 - Hoffmann, Ulrich A1 - Pook, Michael T1 - Simulation von Wärmeübertragungsprozessen T2 - Tagungsband zur AALE-Tagung 2012 : 9. Fachkonferenz Y1 - 2012 SN - 978-3-8356-3305-6 N1 - AALE 2012 SP - 127 EP - 136 PB - Oldenbourg Industrieverlag CY - München ER - TY - JOUR A1 - Schiffels, Johannes A1 - Pinkenburg, Olaf A1 - Schelden, Maximilian A1 - Aboulnaga, El-Hussiny A. A. A1 - Baumann, Marcus A1 - Selmer, Thorsten T1 - An innovative cloning platform enables large-scale production and maturation of an oxygen-tolerant [NiFe]-hydrogenase from cupriavidus necator in Escherichia coli JF - PLOS one. 2013 Y1 - 2013 U6 - http://dx.doi.org/10.1371/journal.pone.0068812 SN - 1932-6203 PB - Public Library of Science CY - San Francisco, California ER - TY - JOUR A1 - Abulnaga, El-Hussiny A1 - Pinkenburg, Olaf A1 - Schiffels, Johannes A1 - E-Refai, Ahmed A1 - Buckel, Wolfgang A1 - Selmer, Thorsten T1 - Effect of an Oxygen-Tolerant Bifurcating Butyryl Coenzyme A Dehydrogenase/Electron-Transferring Flavoprotein Complex from Clostridium difficile on Butyrate Production in Escherichia coli JF - Journal of bacteriology Y1 - 2013 SN - 1098-5530 [E-Journal] SN - 0021-9193 [Print] VL - 195 IS - 16 SP - 3704 EP - 3713 ER - TY - JOUR A1 - Aboulnaga, E. H. A1 - Pinkenburg, O. A1 - Schiffels, Johannes A1 - El-Refai, A. A1 - Buckel, W. A1 - Selmer, Thorsten T1 - Butyrate production in Escherichia coli: Exploitation of an oxygen tolerant bifurcating butyryl-CoA dehydrogenase/electron transferring flavoprotein complex from Clostridium difficile JF - Journal of bacteriology. June 14, 2013 Y1 - 2013 SN - 1098-5530 (E-Journal) ; 0021-9193 (Print) SP - Epub ahead of print ER - TY - JOUR A1 - Winckler, Silvia A1 - Krueger, Rolf A1 - Schnitzler, Thomas A1 - Zang, Werner A1 - Fischer, Rainer A1 - Biselli, Manfred T1 - A sensitive monitoring system for mammalian cell cultivation processes: a PAT approach JF - Bioprocess and biosystems engineering N2 - Biopharmaceuticals such as antibodies are produced in cultivated mammalian cells, which must be monitored to comply with good manufacturing practice. We, therefore, developed a fully automated system comprising a specific exhaust gas analyzer, inline analytics and a corresponding algorithm to precisely determine the oxygen uptake rate, carbon dioxide evolution rate, carbon dioxide transfer rate, transfer quotient and respiratory quotient without interrupting the ongoing cultivation, in order to assess its reproducibility. The system was verified using chemical simulation experiments and was able to measure the respiratory activity of hybridoma cells and DG44 cells (derived from Chinese hamster ovary cells) with satisfactory results at a minimum viable cell density of ~2.0 × 10⁵ cells ml⁻¹. The system was suitable for both batch and fed-batch cultivations in bubble-aerated and membrane-aerated reactors, with and without the control of pH and dissolved oxygen. Y1 - 2014 U6 - http://dx.doi.org/10.1007/s00449-013-1062-8 SN - 1615-7591 (Print) 1615-7605 (Online) VL - 37 IS - 5 SP - 901 EP - 912 PB - Springer CY - Berlin, Heidelberg ER - TY - JOUR A1 - Meyer-Stork, L. Sebastian A1 - Höcker, Hartwig A1 - Berndt, Heinz T1 - Syntheses and reactions of urethanes of cellobiose and cellulose-containing uretdione groups JF - Journal of applied polymer science Y1 - 1992 SN - 1097-4628 VL - 44 IS - 6 SP - 1043 EP - 1049 ER - TY - RPRT A1 - Vaeßen, Christiane A1 - Ohme, H. A1 - Manderscheid, D. T1 - Endbericht Projekt Immotherm : Vorhabensbezeichnung: KMU-innovativ-Verbundvorhaben "Einsatz immobilisierter Mikroorganismen zur Entölung und Entsalzung von Kondensatwasser bei hohen Prozesstemperaturen" : Laufzeit des Vorhabens: 01.03.2009-31.08.2011 : Förderkennzeichen: 01LY0816A, 01LY0816B, 01LY0816C Y1 - 2012 ER - TY - JOUR A1 - Schnitzler, Thomas T1 - Cultivation of hybridoma cell line CF-10H5 (DSMZ ACC477) JF - Application notes / Sartorius stedim biotech Y1 - 2009 SP - 1 EP - 4 ER - TY - JOUR A1 - Schnitzler, Thomas T1 - Kultivierung der Hybridomazelllinie CF-10H5 (DSMZ ACC477) JF - Application notes / Sartorius stedim biotech Y1 - 2009 PB - Sartorius Stedim Biotec CY - Aubagne ER - TY - JOUR A1 - Schnitzler, Thomas A1 - Biselli, Manfred T1 - Cultivo de lineas celulares de hibridoma por CF-10H5 (DSMZ ACC477) en el BIOSTAT B plus JF - Noticias tecnicas del laboratorio Y1 - 2005 VL - 13 IS - 3 SP - 7 EP - 8 ER - TY - JOUR A1 - Hemmerling, H.-J. A1 - Merschenz-Quack, Angelika A1 - Wunderlich, H. T1 - Crystal structures of indeno[1,2-d]imidazoles. XIth European Crystallographic Meeting, Vienna 1988 JF - Zeitschrift für Kristallographie - Crystalline Materials Y1 - 1988 SN - 2196-7105 (E-Books); 2194-4946 (Print) VL - 185 IS - H. 1-4 SP - 256 ER - TY - JOUR A1 - Mootz, D. A1 - Merschenz-Quack, Angelika T1 - Zur Kenntnis der höchsten Hydrate der Schwefelsäure: Bildung und Struktur von H2SO4·6,5 H2O und H2SO4·8H2O JF - Zeitschrift für Naturforschung B: Journal of chemical sciences Y1 - 1987 SN - 1865-7117 (E-Journal); 0932-0776 (Print) VL - 42 IS - H. 10 SP - 1231 EP - 1236 ER - TY - JOUR A1 - Merschenz-Quack, Angelika T1 - Betriebsanweisungen beim Umgang mit Gefahrstoffen JF - Elektro-Praktiker : Fachzeitschrift für Handwerk und Industrie Y1 - 1991 SN - 0013-5569 VL - Bd. 45 IS - H. 10 SP - 644 EP - 649 ER - TY - JOUR A1 - Gerigk, M. A1 - Maaß, D. A1 - Takors, Ralf A1 - Kreutzer, A. A1 - Wandrey, Christian A1 - Bongaerts, Johannes A1 - Wubbolts, Marcel T1 - Fermentative Herstellung von L-Phenylalanin im Fed-Batch Verfahren mit E. coli unter Einbindung eines integrierten Aufarbeitungsverfahrens JF - Chemie-Ingenieur-Technik (CIT) Y1 - 2000 SN - 1522-2640 (E-Journal); 0009-286X (Print) N1 - Printausg. in der Bibliothek vorhanden: 63 ZS 022 VL - Vol. 72 IS - Iss. 9 SP - 926 EP - 927 ER - TY - JOUR A1 - Müller, Ulrike A1 - Bongaerts, Johannes A1 - Bovenberg, Roel A1 - Jossek, Ralf A1 - Krämer, Marco A1 - Linnemann, J. A1 - Müschen, S. A1 - Ritterbecks, S. A1 - Sprenger, G. A1 - Wubbolts, Marcel T1 - Metabolic engineering to produce fine chemicals in Escherichia coli JF - Mededelingen van de Faculteit Landbouwwetenschappen, Rijksuniversiteit Gent Y1 - 2001 SN - 0035-533x VL - 66 (3a) SP - 215 EP - 217 ER - TY - JOUR A1 - Jossek, Ralf A1 - Bongaerts, Johannes A1 - Sprenger, Georg A. T1 - Characterization of a new feedback-resistant 3-deoxy-D-arabinoheptulosonate 7-phosphate synthase AroF of Escherichia coli JF - FEMS microbiology letters Y1 - 2001 SN - 1574-6968 VL - Vol. 202 IS - Iss. 1 SP - 145 EP - 148 ER - TY - JOUR A1 - Bongaerts, Johannes A1 - Krämer, Marco A1 - Müller, Ulrike A1 - Raeven, Leon A1 - Wubbolts, Marcel T1 - Metabolic engineering for microbial production of aromatic amino acids and derived compounds JF - Metabolic engineering Y1 - 2001 SN - 1096-7184 (E-Journal); 1096-7176 (Print) VL - Vol. 3 IS - Iss. 4 SP - 289 EP - 300 ER - TY - JOUR A1 - Schmitz, M. A1 - Hirsch, E. A1 - Bongaerts, Johannes A1 - Takors, Ralf T1 - Pulse experiments as a prerequisite for the quantification of in vivo enzyme kinetics in aromatic amino acid pathway of Eschericia coli JF - Biotechnology progress Y1 - 2002 SN - 1520-6033 (E-Journal); 8756-7938 (Print) VL - Vol. 18 IS - Iss. 5 SP - 935 EP - 941 ER - TY - JOUR A1 - Bongaerts, Johannes A1 - Bovenberg, Roel A1 - Krämer, Marco A1 - Müller, Ulrike A1 - Raeven, Leon A1 - Wubbolts, Marcel T1 - Metabolic engineering to produce fine chemicals in Escherichia coli JF - Chemie - Ingenieur - Technik (CIT) Y1 - 2002 SN - 1522-2640 (E-Journal); 0009-286X (Print) N1 - Printausg. in der Bibliothek vorhanden: 63 ZS 022 VL - Vol. 74 IS - Iss. 5 SP - 694 ER - TY - JOUR A1 - Gerigk, M. A1 - Bujnicki, R. A1 - Ganpo-Nkwenkwa, E. A1 - Bongaerts, Johannes A1 - Sprenger, G. A1 - Takors, Ralf T1 - Process control for enhanced L-phenylalanine production using different recombinant Escherichia coli strains JF - Biotechnology and bioengineering Y1 - 2002 SN - 1097-0290 (E-Journal); 0006-3592 (Print) VL - Vol. 80 IS - Iss. 7 SP - 746 EP - 754 ER - TY - JOUR A1 - Gerigk, M. A1 - Maaß, D. A1 - Kreutzer, A. A1 - Sprenger, G. A1 - Bongaerts, Johannes A1 - Wubbolts, Marcel A1 - Takors, Ralf T1 - Enhanced pilot-scale fed-batch L-phenylalanine production with recombinant Escherichia coli by fully integrated reactive extraction JF - Bioprocess and biosystems engineering Y1 - 2002 SN - 1432-0797 (E-Journal); 1615-7605 (E-Journal); 0178-515X (Print); 1615-7591 (Print) VL - Vol. 25 IS - Iss. 1 SP - 43 EP - 52 ER - TY - JOUR A1 - Krämer, Marco A1 - Bongaerts, Johannes A1 - Bovenberg, Roel A1 - Kremer, Susanne A1 - Müller, Ulrike A1 - Orf, Sonja A1 - Wubbolts, Marcel A1 - Raeven, Leon T1 - Metabolic engineering for microbial production of shikimic acid JF - Metabolic engineering Y1 - 2003 SN - 1096-7184 (E-Journal); 1096-7176 (Print) VL - Vol. 5 IS - Iss. 4 SP - 277 EP - 283 ER - TY - JOUR A1 - Polen, T. A1 - Krämer, Marco A1 - Bongaerts, Johannes A1 - Wubbolts, Marcel A1 - Wendisch, V. F. T1 - The global gene expression response of Escherichia coli to L-phenylalanine JF - Journal of biotechnology Y1 - 2005 SN - 1873-4863 (E-Journal); 0168-1656 (Print) VL - Vol. 115 IS - Iss. 3 SP - 221 EP - 237 ER - TY - CHAP A1 - Özdil, S. A1 - Berndt, Heinz A1 - Höcker, Hartwig T1 - Systematische Erfassung der Beständigkeit von Ryton-, P84- und Dralon ATF 1063-Fasern unter Einwirkung verschiedener NOx-Konzentrationen in einem definierten Temperaturintervall T2 - Schriftenreihe des Deutschen Wollforschungsinstitutes an der Technischen Hochschule Aachen e.V. Y1 - 1991 SN - 0930-3723 IS - 107 SP - 89 EP - 129 PB - Dt. Wollforschungsinstitut CY - Aachen ER - TY - JOUR A1 - Unden, G. A1 - Becker, S. A1 - Bongaerts, Johannes A1 - Schirawski, J. A1 - Six, S. T1 - Oxygen regulated gene expression in facultatively anaerobic bacteria JF - Antonie van Leeuwenhoek Y1 - 1994 SN - 0003-6072 (Print) ; 1572-9699 (online) VL - Vol. 66 IS - Iss. 1-3 SP - 3 EP - 22 ER - TY - JOUR A1 - Bongaerts, Johannes A1 - Zoschke, Sascha A1 - Weidner, Uwe A1 - Linden, Gottfried T1 - Transcriptional regulation of the proton translocating NADH JF - Molecular microbiology Y1 - 1995 SN - 1365-2958 (E-Journal); 0950-382x (Print) VL - Vol. 16 IS - Iss. 3 SP - 521 EP - 534 ER - TY - JOUR A1 - Unden, G. A1 - Becker, S. A1 - Bongaerts, Johannes A1 - Holighaus, G. A1 - Schirawski, J. A1 - Six, S. T1 - O2-sensing and O2-dependent gene regulation in facultatively anaerobic bacteria JF - Archives of microbiology Y1 - 1995 SN - 1432-072X (E-Journal); 0003-9276 (Print); 0302-8933 (Print) VL - Vol. 164 IS - Iss. 2 SP - 81 EP - 90 ER - TY - JOUR A1 - Wissenbach, U. A1 - Six, S. A1 - Bongaerts, Johannes A1 - Ternes, D. A1 - Steinwachs, S. A1 - Unden, G. T1 - A third periplasmic transport system for l-arginine in Escherichia coli: molecular characterization of the artPIQMJ genes, arginine binding and transport JF - Molecular microbiology Y1 - 1995 SN - 1365-2958 (E-Journal); 0950-382x (Print) VL - Vol. 17 IS - Iss. 4 SP - 675 EP - 686 ER - TY - JOUR A1 - Tran, Quang Hon A1 - Bongaerts, Johannes A1 - Vlad, Dorina A1 - Unden, Gottfried T1 - Requirement for the proton-pumping NADH dehydrogenase I of Escherichia coli in respiration of NADH to fumarate and its bioenergetic implications JF - European journal of biochemistry Y1 - 1997 SN - 0014-2956 VL - Vol. 244 IS - Iss. 1 SP - 155 EP - 160 ER - TY - JOUR A1 - Unden, Gottfried A1 - Bongaerts, Johannes T1 - Alternative respiratory pathways of Escherichia coli: energetics and transcriptional regulation in response to electron acceptors JF - Biochimica et biophysica acta (BBA) - Bioenergetics Y1 - 1997 SN - 1879-2650 (E-Journal); 0005-2728 (Print) VL - Vol. 1320 IS - Iss. 3 SP - 217 EP - 234 ER - TY - JOUR A1 - Zientz, Evelyn A1 - Bongaerts, Johannes A1 - Unden, Gottfried T1 - Fumarate regulation of gene expression in Escherichia coli by the DcuSR (dcuSR genes) two-component regulatory system JF - Journal of bacteriology Y1 - 1998 SN - 1098-5530 (E-Journal); 0021-9193 (Print) VL - Vol. 180 IS - No. 20 SP - 5421 EP - 5425 ER - TY - JOUR A1 - Wackwitz, B. A1 - Bongaerts, Johannes A1 - Goodman, S. D. A1 - Unden, Gottfried T1 - Growth phase-dependent regulation of nuoA-N expression in Escherichia coli K-12 by the Fis protein: upstream binding sites and bioenergetic significance JF - Molecular and general genetics : MGG Y1 - 1999 SN - 1617-4623 (E-Journal); 1617-4615 (Print) VL - Vol. 262 IS - Iss. 4 - 5 SP - 876 EP - 883 ER - TY - JOUR A1 - Scheele, S. A1 - Bongaerts, Johannes A1 - Maurer, K.-H. A1 - Freudl, R. T1 - Sekretion einer Kofaktor-haltigen Oxidase durch Corynebacterium glutamicum JF - Chemie - Ingenieur - Technik (CIT) Y1 - 2009 SN - 1522-2640 (E-Journal); 0009-286X (Print) VL - Vol. 81 IS - Iss. 8 SP - 1309 ER - TY - JOUR A1 - Deppe, Veronika Maria A1 - Bongaerts, Johannes A1 - O'Connell, Timothy A1 - Maurer, Karl-Heinz A1 - Meinhardt, Friedhelm T1 - Enzymatic deglycation of Amadori products in bacteria JF - Applied microbiology and biotechnology Y1 - 2011 SN - 1432-0614 (E-Journal); 0171-1741 (Print); 0175-7598 (Print); 0340-2118 (Print) VL - Vol. 90 IS - Iss. 2 SP - 399 EP - 406 PB - Springer CY - Berlin ER - TY - JOUR A1 - Deppe, Veronika Maria A1 - Klatte, Stephanie A1 - Bongaerts, Johannes A1 - Maurer, Karl-Heinz A1 - O'Connell, Timothy A1 - Meinhardt, Friedhelm T1 - Genetic control of Amadori product degradation in Bacillus subtilis via regulation of frlBONMD expression by FrlR JF - Applied and environmental microbiology Y1 - 2011 SN - 1098-5336 (E-Journal); 0003-6919 (Print); 0099-2240 (Print) VL - Vol. 77 IS - No. 9 SP - 2839 EP - 2846 PB - American Society of Mechanical Engineers (ASME) CY - New York ER - TY - JOUR A1 - Bongaerts, Johannes A1 - Esser, Simon A1 - Lorbach, Volker A1 - Al-Momani, Lóay A1 - Müller, Michael A. A1 - Franke, Dirk A1 - Grondal, Christoph A1 - Kurutsch, Anja A1 - Bujnicki, Robert A1 - Takors, Ralf A1 - Raeven, Leon A1 - Wubbolts, Marcel A1 - Bovenberg, Roel A1 - Nieger, Martin A1 - Schürmann, Melanie A1 - Trachtmann, Natalie A1 - Kozak, Stefan A1 - Sprenger, Georg A. A1 - Müller, Michael T1 - Diversity-oriented production of metabolites derived from chorismate and their use in organic synthesis JF - Angewandte Chemie International Edition Y1 - 2011 SN - 1521-3773 (E-Journal); 0570-0833 (Print); 1433-7851 (Print) VL - Vol. 50 IS - Iss. 34 SP - 7781 EP - 7786 PB - Wiley CY - Weinheim ER - TY - JOUR A1 - Borgmeier, Claudia A1 - Bongaerts, Johannes A1 - Meinhardt, Friedhelm T1 - Genetic analysis of the Bacillus licheniformis degSU operon and the impact of regulatory mutations on protease production JF - Journal of biotechnology N2 - Disruption experiments targeted at the Bacillus licheniformis degSU operon and GFP-reporter analysis provided evidence for promoter activity immediately upstream of degU. pMutin mediated concomitant introduction of the degU32 allele – known to cause hypersecretion in Bacillus subtilis – resulted in a marked increase in protease activity. Application of 5-fluorouracil based counterselection through establishment of a phosphoribosyltransferase deficient Δupp strain eventually facilitated the marker-free introduction of degU32 leading to further protease enhancement achieving levels as for hypersecreting wild strains in which degU was overexpressed. Surprisingly, deletion of rapG – known to interfere with DegU DNA-binding in B. subtilis – did not enhance protease production neither in the wild type nor in the degU32 strain. The combination of degU32 and Δupp counterselection in the type strain is not only equally effective as in hypersecreting wild strains with respect to protease production but furthermore facilitates genetic strain improvement aiming at biological containment and effectiveness of biotechnological processes. KW - Marker-free mutagenesis KW - Extracellular enzymes KW - Uracil-phosphoribosyltransferase KW - Hypersecretion Y1 - 2012 U6 - http://dx.doi.org/10.1016/j.jbiotec.2012.02.011 SN - 1873-4863 (E-Journal); 0168-1656 (Print) VL - 159 IS - 1-2 SP - 12 EP - 20 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Scheele, Sandra A1 - Oertel, Dan A1 - Bongaerts, Johannes A1 - Evers, Stefan A1 - Hellmuth, Hendrik A1 - Maurer, Karl-Heinz A1 - Bott, Michael A1 - Freudl, Roland T1 - Secretory production of an FAD cofactor-containing cytosolic enzyme (sorbitol–xylitol oxidase from Streptomyces coelicolor) using the twin-arginine translocation (Tat) pathway of Corynebacterium glutamicum JF - Microbial biotechnology Y1 - 2013 SN - 1751-7915 SP - 202 EP - 206 PB - Wiley-Blackwell CY - Oxford ER - TY - JOUR A1 - Wilming, Anja A1 - Begemann, Jens A1 - Kuhne, Stefan A1 - Regestein, Lars A1 - Bongaerts, Johannes A1 - Evers, Stefan A1 - Maurer, Karl-Heinz A1 - Büchs, Jochen T1 - Metabolic studies of γ-polyglutamic acid production in Bacillus licheniformis by small-scale continuous cultivations JF - Biochemical engineering journal Y1 - 2013 SN - 1873-295X (E-Journal); 1369-703X (Print) VL - Vol. 73 SP - 29 EP - 37 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Voigt, Birgit A1 - Schroeter, Rebecca A1 - Jürgen, Britta A1 - Albrecht, Dirk A1 - Evers, Stefan A1 - Bongaerts, Johannes A1 - Maurer, Karl-Heinz A1 - Schweder, Thomas A1 - Hecker, Michael T1 - The response of Bacillus licheniformis to heat and ethanol stress and the role of the SigB regulon JF - Proteomics Y1 - 2013 SN - 1615-9861 (E-Journal); 1615-9853 (Print) VL - Vol. 13 IS - Iss. 14 SP - 2140 EP - 2146 PB - Wiley CY - Weinheim ER - TY - JOUR A1 - Rachinger, Michael A1 - Bauch, Melanie A1 - Strittmatter, Axel A1 - Bongaerts, Johannes A1 - Evers, Stefan A1 - Maurer, Karl-Heinz A1 - Daniel, Rolf A1 - Liebl, Wolfgang A1 - Liesegang, Heiko A1 - Ehrenreich, Armin T1 - Size unlimited markerless deletions by a transconjugative plasmid-system in Bacillus licheniformis JF - Journal of biotechnology Y1 - 2013 SN - 1873-4863 (E-Journal); 0168-1656 (Print) VL - Vol. 164 IS - Iss. 4 SP - 365 EP - 369 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Wiegand, Sandra A1 - Dietrich, Sascha A1 - Hertel, Robert A1 - Bongaerts, Johannes A1 - Evers, Stefan A1 - Volland, Sonja A1 - Daniel, Rolf A1 - Liesegang, Heiko T1 - RNA-Seq of Bacillus licheniformis: active regulatory RNA features expressed within a productive fermentation JF - BMC genomics Y1 - 2013 SN - 1471-2164 VL - Vol. 14 SP - 667 PB - BioMed Central CY - London ER - TY - CHAP A1 - Berndt, Heinz A1 - Kalbe, Jochen A1 - Kuropka, Rolf A1 - Meyer-Stork, L. Sebastian A1 - Höcker, Hartwig ED - Körner, Andrea T1 - Progress and limitations of the DNA analysis in fine animal fiber identification T2 - Proceedings of the 2nd International Symposium on Specialty Animal Fibers : Aachen, October 19 - 20, 1989. - (Schriftenreihe des Deutschen Wollforschungsinstituts an der Technischen Hochschule Aachen e. V. ; 106) Y1 - 1990 SP - 259 EP - 265 PB - Dt. Wollforschungsinst. CY - Aachen ER - TY - JOUR A1 - Kalbe, Jochen A1 - Höcker, Hartwig A1 - Berndt, Heinz T1 - Design of enzyme reactors as chromatographic columns for racemic resolution of amino acid esters JF - Chromatographia Y1 - 1989 SN - 0009-5893 U6 - http://dx.doi.org/10.1007/BF02319646 VL - 28 IS - 3-4 SP - 193 EP - 196 ER - TY - JOUR A1 - Kuropka, Rolf A1 - Müller, Bettina A1 - Höcker, Hartwig A1 - Berndt, Heinz T1 - Chiral stationary phases via hydrosilylation reaction of N-acryloylamino acids : I. Stationary phase with one chiral centre for high-performance liquid chromatography and development of a new derivatization pattern for amino acid enantiomers JF - Journal of chromatography A Y1 - 1989 SN - 0021-9673 IS - 481 SP - 380 EP - 386 ER - TY - JOUR A1 - Kalbe, Jochen A1 - Kuropka, Rolf A1 - Meyer-Stork, L. Sebastian A1 - Berndt, Heinz A1 - [u.a.], T1 - Isolation and characterization of high-molecular mass DNA from hair shafts JF - Biological chemistry Y1 - 1988 SN - 0177-3593 U6 - http://dx.doi.org/10.1515/bchm3.1988.369.1.413 VL - 369 IS - 1 SP - 413 EP - 416 ER - TY - JOUR A1 - Heinze, Daniel A1 - Mang, Thomas A1 - Peter, Karin A1 - Möller, Martin A1 - Weichold, Oliver T1 - Synthesis of low molecular weight poly(vinyl acetate) and its application as plasticizer JF - Journal of applied polymer science N2 - Poly(vinyl acetate), PVAc, with a degree of polymerization Xn = 10 was prepared by chain-transfer radical polymerization using carbon tetrachloride and used as oligomeric plasticizer for commercial PVAc. However, the chlorinated chain ends cause a low thermal stability requiring mild Cl/H substitution. The product exhibits high thermal stability and excellent melt-compounding properties. Blends of oligomeric and commercial PVAc show single glass transition temperatures which decrease with higher oligomer content and exhibit small negative deviations from Fox' linear additivity rule. This indicates plasticization and miscibility being mainly due to entropic effects. Injection-moulded thick specimens show ductile behaviour at oligomer contents >10 wt %, while sheets with a thickness of 0.2–0.5 mm appear flexible already at 7.5 wt %. The oxygen permeability coefficients are an order of magnitude lower than those of low-density polyethylene. Due to the sum of their properties, the plasticized sheets present a promising alternative in the preparation of barrier materials. Y1 - 2014 U6 - http://dx.doi.org/10.1002/app.40226 SN - 1097-4628 (E-Journal); 0021-8995 (Print) VL - 131 IS - 9 SP - Article No. 40226 PB - Wiley CY - New York ER - TY - JOUR A1 - Dutta, Suryendu A1 - Hartkopf-Fröder, Christoph A1 - Witte, Karin A1 - Brocke, Rainer A1 - Mann, Ulrich T1 - Molecular characterization of fossil palynomorphs by transmission micro-FTIR spectroscopy: implications for hydrocarbon source evaluation JF - International journal of coal geology Y1 - 2013 SN - 1872-7840 (E-Journal); 0166-5162 (Print) VL - Vol. 115 SP - 13 EP - 23 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Meyer-Stork, L. Sebastian A1 - Kalbe, Jochen A1 - Kuropka, Rolf A1 - Sauter, S. L. A1 - Höcker, Hartwig A1 - Berndt, Heinz T1 - Eindeutige Provenienzanalyse von Wolle und feinen Tierhaaren mit Hilfe der Erbsubstanz (DNS) JF - Textilveredlung : schweizerische Zeitschrift für Textilchemie, Textilveredlung und deren Randgebiete Y1 - 1988 SN - 0040-5310 VL - 23 IS - 9 SP - 304 EP - 307 ER - TY - CHAP A1 - Takenaga, Shoko A1 - Herrera, Cony F. A1 - Werner, Frederik A1 - Biselli, Manfred A1 - Schnitzler, Thomas A1 - Schöning, Michael Josef A1 - Öhlschläger, Peter A1 - Wagner, Torsten T1 - Detection of the metabolic activity of cells by differential measurements based on a single light-addressable potentiometric sensor chip T2 - 11. Dresdner Sensor-Symposium : 9.-11.12.2013 Y1 - 2013 SN - 978-3-9813484-5-3 SP - 63 EP - 67 ER - TY - JOUR A1 - Krüger, Götz A1 - Grötzinger, Joachim A1 - Berndt, Heinz T1 - Enantiomeric resolution of amino acid derivatives on chiral stationary phases by high-performance liquid chromatography JF - Journal of Chromatography A Y1 - 1987 U6 - http://dx.doi.org/10.1016/S0021-9673(01)85005-6 SN - 0021-9673 VL - 1987 IS - 397 SP - 223 EP - 232 ER - TY - GEN A1 - Krämer, David T1 - Development of a gene filter array protocol for studies of gene regulation by AMP activated protein kinase (AMPK) Y1 - 2002 ER - TY - JOUR A1 - Berndt, Heinz A1 - Klostermeyer, Henning A1 - Zahn, Helmut T1 - Zur Synthese monomerer cyclischer Cystinpeptidderivate, I : Synthese der Sequenz A 6–9 des Schafinsulins als Cyclocystinylderivat JF - Justus Liebigs Annalen der Chemie N2 - Es wird die Synthese der Sequenz A 6–9 des Schafinsulins in der geschützten Form Boc-Cys-Cys-Ala-Gly-OBuᵗ (5) sowie das Verhalten dieses monomeren cyclischen Cystinpeptidderivates gegenüber den in der Peptidchemie gebräuchlichen Reagenzien Bortrifluorid/Eisessig, Triäthylamin und Hydrazinhydrat beschrieben. Y1 - 1972 U6 - http://dx.doi.org/10.1002/jlac.19727590109 SN - 1099-0690 VL - 759 IS - 1 SP - 114 EP - 120 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Schnabel, Eugen A1 - Schnabel, Henning A1 - Berndt, Heinz T1 - Zur selektiven acidolytischen Abspaltbarkeit der tert.-Butyloxycarbonyl-Gruppe JF - Justus Liebigs Annalen der Chemie N2 - Die tert.-Butyloxycarbonyl-Gruppe (Boc) läßt sich mittels reiner Trifluoressigsäure nicht selektiv neben dem Benzyloxycarbonyl-Rest (Z) abspalten. Das gelingt auch nicht mit Lösungen von Trifluoressigsäure bzw. Chlorwasserstoff in organischen Lösungsmitteln. Kern-substituierte Z-Gruppen wie Z(pCl), Z(mCl) oder Z(pNO₂) sind zwar stabiler, werden aber von den obengenannten Reagenzien ebenfalls angegriffen bzw. sind nicht mehr acidolytisch abspaltbar. – Mit 70proz. wäßriger Trifluoressigsäure gelingt die Abspaltung von Boc neben Z dagegen fast selektiv; dabei werden aber Benzylester, besonders Glutaminsäure-γ-benzylester, teilweise hydrolysiert, während Methyl- sowie Äthylester nahezu beständig sind. Die Brauchbarkeit des Abspaltungsverfahrens wird anhand der schrittweise durchgeführten Synthese zweier Heptapeptid-Derivate gezeigt. – Ähnlich spezifisch gelingt die Abspaltung von Boc mit Bortrifluorid-ätherat in Eisessig; Benzylester sind gegenüber diesem Reagenz stabiler als gegen wäßrige Trifluoressigsäure. Das Bortrifluorid-Verfahren eignet sich besonders für die Abspaltung von Boc-Gruppen neben säurelabilen Thiol-Schutzgruppen (Tetrahydropyranyl- bzw. Trityl-Rest) sowie neben dem Cyclocystinyl-Rest. Die Leistungsfähigkeit der Methode wird durch die Synthese zweier Peptid-Derivate mit S-Trityl-Schutzgruppen belegt. Als Nebenreaktion ist die Acetylierung von aliphatischen Hydroxylgruppen möglich. Sie läßt sich vermeiden, wenn man die Spaltung in anderen Lösungsmitteln durchführt. Die als Modellverbindungen für Stabilitätsuntersuchungen verwendeten Nε-acylierten Lysin-Derivate werden mit dem Aminosäureanalysator quantitativ neben Lysin bestimmt. Y1 - 1971 U6 - http://dx.doi.org/10.1002/jlac.19717490111 SN - 1099-0690 VL - 749 IS - 1 SP - 90 EP - 108 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Nokihara, Kiyoshi A1 - Berndt, Heinz T1 - Synthesis of hapten–polypeptide conjugates as antigen models for the N-terminal region of the α-2-chain of rabbit skin collagen JF - Journal of the Royal Society of Chemistry: Perkin Transactions 1 N2 - Synthesis of derivatives of the peptide sequence L-pyroglutamyl-L-phenylalanyl-L-aspartyl-glycyl-L-lysyl-glycyl-glycyl-glycine as the antigenic determinant representing the N-terminal non-helical region of the α-2-chain of rabbit skin collagen, and conjugation to two different polypeptide carriers, are described. Y1 - 1978 U6 - http://dx.doi.org/10.1039/P19780000260 SN - 1364-5463 SN - 0300-922X SN - 1470-4358 VL - 1978 IS - 3 SP - 260 EP - 263 PB - Royal Society of Chemistry CY - Cambridge ER - TY - CHAP A1 - Berndt, Heinz A1 - Turck, Christoph W. ED - Voelter, Wolfgang T1 - Syntheses of defined peptide derivatives by aminolysis of 3-[Nα-benzyloxycarbonyl peptidyloxy] -2-hydroxy-N-methyl-benzamides at elevated temperatures II. Synthesis of the peptide derivatives Z-Ala-X-Gly-N(Et)2, X=Phe, Leu, Val, Ser (But), Glu (OBut) T2 - Chemistry of peptides and proteins : proceedings of the Fourth USSR-FRG Symposium, Tübingen, Federal Republic of Germany, June 8 - 12, 1982 / ed. Wolfgang Voelter ... - Vol. 2 Y1 - 1984 SN - 3-11009-580-7 SP - 97 EP - 103 PB - de Gruyter CY - Berlin [u.a.] ER - TY - CHAP A1 - Zahn, Helmut A1 - Berndt, Heinz A1 - Fehrenbach, P. ED - Hanson, Horst T1 - Synthese cyclischer Cystinpeptide mit Insulin A-und B-Kettensequenzen T2 - Peptides 1972 : proceedings of the Twelfth European Peptide Symposium, Reinhardsbrunn Castle, German Democratic Republic, September 1972 Y1 - 1973 SN - 0-7204-4132-3 SN - 0-444-10500-X SP - 101 EP - 102 PB - North-Holland Publ. [u.a.], CY - Amsterdam [u.a.] ER - TY - CHAP A1 - Schnabel, Eberhard A1 - Berndt, Heinz ED - Nesvadba, H. T1 - Zur selektive Abspaltbarkeit der t-Butyloxycarbonylgruppe T2 - Peptides 1971 : proceedings of the Eleventh European Peptide Symposium, Vienna, Austria, April 1971 Y1 - 1973 SN - 0-7204-4120-X SP - 69 EP - 70 PB - North-Holland Publ. [u.a.] CY - Amsterdam [u.a.] ER - TY - CHAP A1 - Kroggel, Matthias A1 - Berndt, Heinz ED - Ragnarsson, Ulf T1 - The 0-hydroxiphenyloxicarbonyl-group a new base labile amine protecting group T2 - Peptides 1984 : Proceedings of the 18th European Peptide Symposium Djurönäset, Sweden, June 10 - 15, 1984 Y1 - 1984 SN - 91-22-00715-6 SP - 81 EP - 83 PB - Almquist & Wiksell CY - Stockholm ER - TY - THES A1 - Berndt, Heinz T1 - Zur Synthese der Sequenz 60-84[Ala71, Gly72, Val73] des Schweineproinsulins als monomeres cyclisches Bis-Cystinpeptidderivat Y1 - 1972 N1 - Aachen, Techn. Hochsch., Diss. 1972 CY - Aachen ER - TY - BOOK A1 - Berndt, Heinz T1 - Neue Kondensations-Methoden zur Synthese definierter Peptid-Derivate Y1 - 2014 N1 - Aachen, Techn. Hochsch., Habil.-Schr., 1982 CY - Aachen ER - TY - JOUR A1 - Nokihara, Kiyoshi A1 - Berndt, Heinz T1 - Darstellung von Bis(S-methoxycarbonylthio)-B-Kette des Rinderinsulins JF - Hoppe-Seyler's Zeitschrift für physiologische Chemie Y1 - 1979 U6 - http://dx.doi.org/10.1515/bchm2.1979.360.1.773 SN - 1437-4315 SN - 0018-4888 VL - 360 IS - 1 SP - 773 EP - 776 ER - TY - JOUR A1 - Berndt, Heinz T1 - Synthese der Sequenz 71—86 des Humanproinsulins, III : Synthese über die Fragmente 71—78 und 79—86 JF - Hoppe-Seyler's Zeitschrift für physiologische Chemie Y1 - 1979 U6 - http://dx.doi.org/10.1515/bchm2.1979.360.1.765 SN - 1437-4315 SN - 0018-4888 VL - 360 IS - 1 SP - 765 EP - 772 ER - TY - JOUR A1 - Berndt, Heinz T1 - Synthese der Sequenz 71—86 des Humanproinsulins, I : Synthese der Sequenz 71—86 als monomeres cyclisches Biscystinpeptidderivat und als Tetra-S-tritylderivat JF - Hoppe-Seyler's Zeitschrift für physiologische Chemie Y1 - 1979 U6 - http://dx.doi.org/10.1515/bchm2.1979.360.1.747 SN - 1437-4315 SN - 0018-4888 VL - 360 IS - 1 SP - 747 EP - 760 ER - TY - JOUR A1 - Naithani, V. K A1 - Klostermeyer, Henning A1 - Lange, H. R. A1 - [u.a.], A1 - Berndt, Heinz A1 - [u.a.], T1 - Preparation of peptide derivatives for porcine proinsulin-synthesis JF - Biological Chemistry Y1 - 1971 U6 - http://dx.doi.org/10.1515/bchm2.1971.352.1.1 SN - 1437-4315 VL - 352 IS - 1 SP - 2 EP - 3 PB - De Gruyter ER - TY - JOUR A1 - Matoni, Georg A1 - Berndt, Heinz T1 - Thermal synthesis of the optical pure pentapeptide derivative Z-(L)-Ala-(L)-Phe-Gly-(L)-Phe-Gly-OMe JF - Tetrahedron letters Y1 - 1980 U6 - http://dx.doi.org/10.1016/S0040-4039(00)93618-9 SN - 0040-4039 VL - 21 IS - 1 SP - 37 EP - 40 ER - TY - JOUR A1 - Nokihara, Kiyoshi A1 - Berndt, Heinz T1 - Studies on sulfur-containing peptides : tert-butyloxycarbonylsulfenyl and benzyloxycarbonylsulfenyl derivatives as protecting groups for cysteine JF - The journal of organic chemistry Y1 - 1978 U6 - http://dx.doi.org/10.1021/jo00419a046 SN - 0022-3263 VL - 43 IS - 25 SP - 4893 EP - 4895 PB - American Chemical Society CY - Washington ER - TY - JOUR A1 - Berndt, Heinz A1 - Gattner, Hans-Gregor A1 - Zahn, Helmut T1 - Semisynthetisches Des-A1-glycin-Schafinsulin JF - Biological Chemistry Y1 - 1975 U6 - http://dx.doi.org/10.1515/bchm2.1975.356.2.1455 SN - 1437-4315 VL - 356 IS - 2 SP - 1469 EP - 1472 PB - De Gruyter CY - Berlin ER - TY - JOUR A1 - Berndt, Heinz A1 - Zahn, Helmut T1 - Peptide, 99 : Monomere cyclische Cystinpeptidderivate, III ; Synthese der Schafinsulin-A-Kettensequenzen A2–21 und A1–21 als monomere cyclische Dicystinpeptidderivate JF - Justus Liebigs Annalen der Chemie N2 - Die Synthese der Sequenzen A2—21 (13) und A1—21 (15) der Schafinsulin-A-Kette als monomere cyclische Dicystinpeptidderivate wird beschrieben. Die intrachenaren Cystinbrücken A6—7 und A 11 —20 vermitteln die Löslichkeit dieser Derivate in Dimethylformamid und ermöglichen erstmalig die Reindarstellung vollgeschützter Insulin-A-Kettenderivate. Die während der Synthese eingesetzten Schutzgruppen lassen sich mittels Trifluoressigsäure und 2-Mercaptoäthanol quantitativ entfernen. Y1 - 1975 U6 - http://dx.doi.org/10.1002/jlac.197519750908 SN - 1099-0690 VL - 1975 IS - 9 SP - 1601 EP - 1612 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Schwertner, Eberhard A1 - Berndt, Heinz A1 - Gielen, Hans-Günter A1 - Zahn, Helmut T1 - Peptide 96 : Synthese einiger [2-(p-Biphenylyl)isopropyloxycarbonyl]-Aminosäurederivate JF - Justus Liebigs Annalen der Chemie N2 - Die Darstellung der N-[2-(p-Biphenylyl)isopropyloxycarbonyl]-Derivate (Bpoc-Derivate) des Cysteins unter Verwendung der Thiolschutzgruppen Tetrahydropyranyl (Thp) für 1, Diphenylmethyl (Dpm) für 2, Trityl (Trt) für 3 und S-tert.-Butyl (SBut) für 4 sowie die Synthese von aktivierten Estern der Bpoc-Derivate des Glycins (5), Isoleucins (6) und Prolins (7) werden beschrieben. An einem Beispiel wird die Möglichkeit aufgezeigt, die Bpoc-Gruppe über das Bpoc-Azid nachträglich in den Peptidverband einzuführen. Y1 - 1975 U6 - http://dx.doi.org/10.1002/jlac.197519750318 SN - 1099-0690 VL - 75 IS - 3 SP - 581 EP - 585 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Turck, Christoph W. A1 - Berndt, Heinz T1 - Synthese definierter Peptid-Derivate durch Aminolyse von 3-(Nα-Acyl-peptidyloxy)-2-hydroxy-N-alkylbenzamiden bei erhöhten Temperaturen, I : Synthese des Modellpeptid-Derivates Z-Ala-Phe-Gly-N(Et)2 JF - Hoppe-Seyler´s Zeitschrift für physiologische Chemie Y1 - 1981 U6 - http://dx.doi.org/10.1515/bchm2.1981.362.1.821 SN - 1437-4315 SN - 0018-4888 VL - 362 IS - 1 SP - 821 EP - 828 ER - TY - JOUR A1 - Danho, Waleed A1 - Naithani, Vinod K. A1 - Sasaki, André N. A1 - Föhles, Joseph A1 - Berndt, Heinz A1 - [u.a.], T1 - Human proinsulin, VII : synthesis of two protected peptides corresponding to the sequences 1—45 and 46—86 of the prohormone JF - Hoppe-Seyler's Zeitschrift für physiologische Chemie Y1 - 1980 U6 - http://dx.doi.org/10.1515/bchm2.1980.361.1.857 SN - 1437-4315 SN - 0018-4888 VL - 361 IS - 1 SP - 857 EP - 863 ER - TY - JOUR A1 - Wolf, Günter A1 - Berndt, Heinz A1 - Brandenburg, Dietrich T1 - Synthese der [LysA13] Rinderinsulin-A-Kette in der Form [Lys(Tfa)A13]A(SO3H)4 und NαA1-Msc-[LysA13]A(SO3H)4 unter Verwendung des S-tert-Butylmercapto-Restes als Thiolschutzgruppe JF - Hoppe-Seyler's Zeitschrift für physiologische Chemie Y1 - 1979 U6 - http://dx.doi.org/10.1515/bchm2.1979.360.2.1569 SN - 1437-4315 SN - 0018-4888 VL - 360 IS - 2 SP - 1569 EP - 1578 ER - TY - JOUR A1 - Wilhelm, Wolff A1 - Berndt, Heinz A1 - Brandenburg, Dietrich T1 - Zur Synthese der Hühnerinsulin-A-Kette, I : Darstellung der Fragmente A1–8, A9–15, A1–7 und A8–15 JF - Hoppe-Seyler's Zeitschrift für physiologische Chemie Y1 - 1979 U6 - http://dx.doi.org/10.1515/bchm2.1979.360.2.1559 SN - 1437-4315 SN - 0018-4888 VL - 360 IS - 2 SP - 1559 EP - 1568 ER - TY - JOUR A1 - Wolf, Wilhelm A1 - Berndt, Heinz A1 - Brandenburg, Dietrich T1 - Synthese von Fragmenten einer [LysA13] Rinder-Insulin-A-Kette unter Verwendung des S-tert-Butylmercaptorestes als Thiolschutz JF - Hoppe-Seyler's Zeitschrift für physiologische Chemie Y1 - 1979 U6 - http://dx.doi.org/10.1515/bchm2.1979.360.2.1549 SN - 1437-4315 SN - 0018-4888 VL - 360 IS - 2 SP - 1549 EP - 1558 ER - TY - JOUR A1 - Gielen, Hans-Günther A1 - Wolf, Günter A1 - Berndt, Heinz A1 - Zahn, Helmut T1 - Synthese der Fragmente A1–8, A9–15 und A16–21 der Schafinsulin-A-Kette unter Verwendung des S-tert-Butylmercaptorestes als Thiolschutzgruppe JF - Hoppe-Seyler's Zeitschrift für physiologische Chemie Y1 - 1979 U6 - http://dx.doi.org/10.1515/bchm2.1979.360.2.1535 SN - 1437-4315 SN - 0018-4888 VL - 360 IS - 2 SP - 1535 EP - 1548 ER - TY - CHAP A1 - Berndt, Heinz A1 - Kalbe, Jochen A1 - Kuropka, Rolf A1 - Meyer-Stork, L. Sebastian A1 - Sauter, S. L. A1 - Höcker, Hartwig T1 - Eindeutige Charakterisierung von Tierhaaren und Tierhaar-Mischungen durch DNA-Analytik T2 - Beiträge zur 15. Gemeinsamen Tagung der Aachener Textilforschungsinstitute und des "Wollfadens", zugleich 32. Arbeitstagung des Deutschen Wollforschungsinstitutes an der Technischen Hochschule Aachen : am 06. und 07. Oktober 1988. - (Schriftenreihe des Deutschen Wollforschungsinstituts an der Technischen Hochschule Aachen e. V. ; 104) Y1 - 1989 SN - 0930-3723 SP - 29 EP - 32 PB - Dt. Wollforschungsinst. CY - Aachen ER - TY - CHAP A1 - Kalbe, Jochen A1 - Kuropka, Rolf A1 - Meyer-Stork, L. Sebastian A1 - Lauter, S. L. A1 - Höcker, Hartwig A1 - Berndt, Heinz ED - Körner, Andrea T1 - Identification of fine animal hair via DNA analysis T2 - Proceedings of the 1st International Symposium on Specialty Animal Fibers : Aachen, October 26 - 27, 1987 ; [scientific, technological and economical aspects] . - (Schriftenreihe des Deutschen Wollforschungsinstitutes an der Technischen Hochschule Aachen e.V. ; 103) Y1 - 1988 SN - 0930-3723 SP - 221 EP - 227 PB - Dt. Wollforschungsinst. CY - Aachen ER - TY - JOUR A1 - Berndt, Heinz A1 - Krüger, Götz T1 - Resolution of enantiomeric amino acid derivatives by high-performance liquid chromatography on chiral stationary phases JF - Journal of chromatography A Y1 - 1985 U6 - http://dx.doi.org/10.1016/S0021-9673(01)92461-6 SN - 0021-9673 VL - 1985 IS - 348 SP - 275 EP - 279 ER - TY - PAT A1 - Berndt, Heinz A1 - Höcker, Hartwig A1 - Kuropka, Rolf A1 - Kinkel, Joachim T1 - Silanderivate : Europäische Patenschrift / Offenlegungsschrift T1 - Silane derivatives : Europäische Patenschrift / Offenlegungsschrift Y1 - 1991 PB - Europäisches Patentamt / Deutsches Patent- und Markenamt CY - Den Hague / München ER - TY - PAT A1 - Berndt, Heinz A1 - Höcker, Hartwig A1 - Kuropka, Rolf A1 - Kinkel, Joachim T1 - Silane coated inorganic materials for chromatography : United States Patent Y1 - 1991 PB - [The United States Patent and Trademark Office] CY - [Alexandria, VA u.a.] ER - TY - JOUR A1 - Wang, Ren-Qi A1 - Druckenmüller, Katharina A1 - Elbers, Gereon A1 - Guenther, Klaus A1 - Croué, Jean-Philippe T1 - Analysis of aquatic-phase natural organic matter by optimized LDI-MS method JF - Journal of mass spectrometry N2 - The composition and physiochemical properties of aquatic-phase natural organic matter (NOM) are most important problems for both environmental studies and water industry. Laser desorption/ionization (LDI) mass spectrometry facilitated successful examinations of NOM, as humic and fulvic acids in NOM are readily ionized by the nitrogen laser. In this study, hydrophobic NOMs (HPO NOMs) from river, reservoir and waste water were characterized by this technique. The effect of analytical variables like concentration, solvent composition and laser energy was investigated. The exact masses of small molecular NOM moieties in the range of 200–1200 m/z were determined in reflectron mode. In addition, spectra of post-source-decay experiments in this range showed that some compounds from different natural NOMs had the same fragmental ions. In the large mass range of 1200–15 000 Da, macromolecules and their aggregates were found in HPO NOMs from natural waters. Highly humic HPO exhibited mass peaks larger than 8000 Da. On the other hand, the waste water and reservoir water mainly had relatively smaller molecules of about 2000 Da. The LDI-MS measurements indicated that highly humic river waters were able to form large aggregates and membrane foulants, while the HPO NOMs from waste water and reservoir water were unlikely to form large aggregates. Copyright © 2014 John Wiley & Sons, Ltd. Y1 - 2014 U6 - http://dx.doi.org/10.1002/jms.3321 SN - 1096-9888 VL - 49 IS - 2 SP - 154 EP - 160 PB - Wiley CY - Bognor Regis ER - TY - JOUR A1 - Heine, A. A1 - Herrmann, G. A1 - Selmer, Thorsten A1 - Terwesten, F. A1 - Buckel, W. A1 - Reuter, K. T1 - High resolution crystal structure of clostridium propionicum β-Alanyl-CoA:Ammonia Lyase, a new member of the "Hot Dog Fold" protein superfamily JF - Proteins N2 - Clostridium propionicum is the only organism known to ferment β-alanine, a constituent of coenzyme A (CoA) and the phosphopantetheinyl prosthetic group of holo-acyl carrier protein. The first step in the fermentation is a CoA-transfer to β-alanine. Subsequently, the resulting β-alanyl-CoA is deaminated by the enzyme β-alanyl-CoA:ammonia lyase (Acl) to reversibly form ammonia and acrylyl-CoA. We have determined the crystal structure of Acl in its apo-form at a resolution of 0.97 Å as well as in complex with CoA at a resolution of 1.59 Å. The structures reveal that the enyzme belongs to a superfamily of proteins exhibiting a so called “hot dog fold” which is characterized by a five-stranded antiparallel β-sheet with a long α-helix packed against it. The functional unit of all “hot dog fold” proteins is a homodimer containing two equivalent substrate binding sites which are established by the dimer interface. In the case of Acl, three functional dimers combine to a homohexamer strongly resembling the homohexamer formed by YciA-like acyl-CoA thioesterases. Here, we propose an enzymatic mechanism based on the crystal structure of the Acl·CoA complex and molecular docking. Proteins 2014; 82:2041–2053. © 2014 Wiley Periodicals, Inc. Y1 - 2014 U6 - http://dx.doi.org/10.1002/prot.24557 SN - 1097-0134 (E-Journal); 0887-3585 (Print) VL - 82 IS - 9 SP - 2041 EP - 2053 PB - Wiley-Liss CY - New York ER - TY - JOUR A1 - Baumann, Marcus A1 - Gronsfeld, A. A1 - Schütz-Lembach, G. T1 - Nachdenklichkeit über Nachhaltigkeit : wie uns ein neues Wort alte Probleme neu entdecken läßt JF - Wechselwirkung : Wissenschaft & vernetztes Denken Y1 - 1997 SN - 0172-1623 VL - 19 IS - 87 SP - 6 EP - 11 ER - TY - JOUR A1 - Baumann, Marcus A1 - Peschel, G. T1 - Aktuelle Entwicklungen zur Luftreinhaltung in der Stadt Aachen JF - Wechselwirkung : Wissenschaft & vernetztes Denken Y1 - 1998 SN - 0172-1623 VL - 20 IS - 91 SP - 22 EP - 29 ER - TY - JOUR A1 - Wiezorek, E. A1 - Baumann, Marcus T1 - Stadtökologie in der Stadtplanung: der "Stadtökologische Beitrag" Aachen JF - Wechselwirkung : Wissenschaft & vernetztes Denken Y1 - 1999 SN - 0172-1623 VL - 21 IS - 97 SP - 28 EP - 37 ER - TY - JOUR A1 - Schöning, Michael Josef A1 - Biselli, Manfred A1 - Selmer, Thorsten A1 - Öhlschläger, Peter A1 - Baumann, Marcus A1 - Förster, Arnold A1 - Poghossian, Arshak T1 - Forschung „zwischen“ den Disziplinen: das Institut für Nano- und Biotechnologien JF - Analytik news : das Online-Labormagazin für Labor und Analytik N2 - "Biologie trifft Mikroelektronik", das Motto des Instituts für Nano- und Biotechnologien (INB) an der FH Aachen, unterstreicht die zunehmende Bedeutung interdisziplinär geprägter Forschungsaktivitäten. Der thematische Zusammenschluss grundständiger Disziplinen, wie die Physik, Elektrotechnik, Chemie, Biologie sowie die Materialwissenschaften, lässt neue Forschungsgebiete entstehen, ein herausragendes Beispiel hierfür ist die Nanotechnologie: Hier werden neue Werkstoffe und Materialien entwickelt, einzelne Nanopartikel oder Moleküle und deren Wechselwirkung untersucht oder Schichtstrukturen im Nanometerbereich aufgebaut, die neue und vorher nicht bekannte Eigenschaften hervorbringen. Vor diesem Hintergrund bündelt das im Jahre 2006 gegründete INB die an der FH Aachen vorhandenen Kompetenzen von derzeit insgesamt sieben Laboratorien auf den Gebieten der Halbleitertechnik und Nanoelektronik, Nanostrukturen und DNA-Sensorik, der Chemo- und Biosensorik, der Enzymtechnologie, der Mikrobiologie und Pflanzenbiotechnologie, der Zellkulturtechnik, sowie der Roten Biotechnologie synergetisch. In der Nano- und Biotechnologie steckt außergewöhnliches Potenzial! Nicht zuletzt deshalb stellen sich die Forscher der Herausforderung, in diesem Bereich gemeinsam zu forschen und Schnittstellen zu nutzen, um so bei der Gestaltung neuartiger Ideen und Produkte mitzuwirken, die zukünftig unser alltägliches Leben verändern werden. Im Folgenden werden die verschiedenen Forschungsbereiche kurz zusammenfassend vorgestellt und vorhandene Interaktionen anhand von exemplarisch ausgewählten, aktuellen Forschungsprojekten skizziert. Y1 - 2012 VL - Publ. online PB - Dr. Beyer Internet-Beratung CY - Ober-Ramstadt ER - TY - JOUR A1 - Takenaga, Shoko A1 - Biselli, Manfred A1 - Schnitzler, Thomas A1 - Öhlschläger, Peter A1 - Wagner, Torsten A1 - Schöning, Michael Josef T1 - Toward multi-analyte bioarray sensors: LAPS-based on-chip determination of a Michaelis–Menten-like kinetics for cell culturing JF - Physica status solidi A : Applications and materials science N2 - The metabolic activity of Chinese hamster ovary (CHO) cells was observed using a light-addressable potentiometric sensor (LAPS). The dependency toward different glucose concentrations (17–200 mM) follows a Michaelis–Menten kinetics trajectory with Kₘ = 32.8 mM, and the obtained Kₘ value in this experiment was compared with that found in literature. In addition, the pH shift induced by glucose metabolism of tumor cells transfected with the HPV-16 genome (C3 cells) was successfully observed. These results indicate the possibility to determine the tumor cells metabolism with a LAPS-based measurement device. Y1 - 2014 U6 - http://dx.doi.org/10.1002/pssa.201330464 SN - 1521-396X (E); 1862-6319 (E-Journal); 0031-8965 (Print); 1862-6300 (Print) VL - 211 IS - 6 SP - 1410 EP - 1415 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Whitehead, Mark A1 - Öhlschläger, Peter A1 - Almajhdi, Fahad N. A1 - Alloza, Leonor A1 - Marzábal, Pablo A1 - Meyers, Ann E. A1 - Hitzeroth, Inga I. A1 - Rybicki, Edward P. T1 - Human papillomavirus (HPV) type 16 E7 protein bodies cause tumour regression in mice JF - BMC cancer Y1 - 2014 U6 - http://dx.doi.org/10.1186/1471-2407-14-367 SN - 1471-2407 IS - 14:367 SP - 1 EP - 15 PB - BioMed Central CY - London ER - TY - JOUR A1 - Küppers, Tobias A1 - Steffen, Victoria A1 - Hellmuth, Hendrik A1 - O'Connell, Timothy A1 - Bongaerts, Johannes A1 - Maurer, Karl-Heinz A1 - Wiechert, Wolfgang T1 - Developing a new production host from a blueprint: Bacillus pumilus as an industrial enzyme producer JF - Microbial cell factories Y1 - 2014 U6 - http://dx.doi.org/10.1186/1475-2859-13-46 SN - 1475-2859 (E-Journal) VL - 13 SP - Article No. 46 PB - BioMed Central CY - London ER - TY - JOUR A1 - Guo, Yuanyuan A1 - Miyamoto, Ko-ichiro A1 - Wagner, Torsten A1 - Schöning, Michael Josef A1 - Yoshinobu, Tatsuo T1 - Theoretical study and simulation of light-addressable potentiometric sensors JF - Physica status solidi (A) : applications and materials N2 - The light-addressable potentiometric sensor (LAPS) is a semiconductor-based potentiometric sensor using a light probe with an ability of detecting the concentration of biochemical species in a spatially resolved manner. As an important biomedical sensor, research has been conducted to improve its performance, for instance, to realize high-speed measurement. In this work, the idea of facilitating the device-level simulation, instead of using an equivalent-circuit model, is presented for detailed analysis and optimization of the performance of the LAPS. Both carrier distribution and photocurrent response have been simulated to provide new insight into both amplitude-mode and phase-mode operations of the LAPS. Various device parameters can be examined to effectively design and optimize the LAPS structures and setups for enhanced performance. Y1 - 2014 U6 - http://dx.doi.org/10.1002/pssa.201330354 SN - 0031-8965 VL - 211 IS - 6 SP - 1467 EP - 1472 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Smith, Walker O. A1 - Baumann, Marcus A1 - Wilson, David L. A1 - Aletsee, Ludwig T1 - Phytoplankton biomass and productivity in the marginal ice zone of the Fram Strait during summer 1984 JF - Journal of geophysical research Y1 - 1987 SN - 2156-2202 (E-Journal); 2169-9291 (E-Journal); 0148-0227 (Print); 2169-9275 (Print) VL - Vol. 92 IS - Iss. C7 SP - 6777 EP - 6786 ER - TY - JOUR A1 - Jahnke, J. A1 - Baumann, Marcus T1 - Differentiation between Phaeocystis pouchetii (Har.) Lagerheim and Phaeocystis globosa Scherffel JF - Hydrobiological bulletin Y1 - 1987 SN - 0165-1404 (Print); 1573-5125 (E-Journal) VL - Vol. 21 IS - Iss. 2 SP - 141 EP - 147 ER - TY - CHAP A1 - Baumann, Marcus ED - Hempel, G. T1 - Primary production and phytoplankton growth in the marginal ice zone of the Fram Strait and the interpretation of the field data with autecological experiments (Abstract) T2 - Oceanography and biology of Arctic seas : selected papers from the ICES Symposium on Marine Sciences of the Arctic and Subarctic Regions, held in Santander, 28 - 30 September 1987. (Rapports et procès-verbaux des réunions / Conseil International pour l'Exploration de la Mer ; 188) Y1 - 1989 SP - 128 PB - International Council for the Exploration of the Sea CY - Copenhagen ER - TY - JOUR A1 - Aletsee, Ludwig A1 - Baumann, Marcus T1 - A laboratory incubator equipped with facilities to automatically simulate natural irradiance JF - Boletim do Instituto Oceanográfico / Universidade de São Paulo Y1 - 1991 SN - 1982-436X; 0080-6331 VL - Vol. 39 IS - No. 2 SP - 155 EP - 159 ER - TY - JOUR A1 - Tüg, Helmut A1 - Baumann, Marcus T1 - Problems of UV-B radiation measurements in biological research : critical remarks on current techniques and suggestions for improvements JF - Geophysical research letters Y1 - 1994 SN - 1944-8007 (E-Journal); 0094-8276 (Print) VL - Vol. 21 IS - Iss. 8 SP - 689 EP - 692 ER - TY - JOUR A1 - Medlin, L. K. A1 - Lange, M. A1 - Baumann, Marcus T1 - Genetic differentiation among three colony-forming species of Phaeocystis : further evidence for the phylogeny of the Prymnesiophyta JF - Phycologia Y1 - 1994 SN - 0031-8884 VL - Vol. 33 IS - Iss. 3 SP - 199 EP - 212 ER - TY - CHAP A1 - Medlin, L. K. A1 - Barker, G. L. A. A1 - Baumann, Marcus A1 - Hayes, P. K. T1 - Molecular biology and systematics T2 - The Haptophyte Algae (Special volume / Systematics Association : 51) Y1 - 1994 SN - 0-19-857772-9 SP - 393 EP - 411 PB - Clarendon Press CY - Oxford ER - TY - JOUR A1 - Tüg, Helmut A1 - Baumann, Marcus T1 - Reply to the comments by R.L. McKenzie and P.V. Johnston on our paper “Problems of UV-B radiation measurements in biological research: Critical Remarks on current techniques and suggestions for improvements” JF - Geophysical research letters Y1 - 1995 SN - 1944-8007 (E-Journal); 0094-8276 (Print) VL - Vol. 22 IS - Iss. 9 SP - 1159 EP - 1160 ER - TY - CHAP A1 - Brandini, Frederico P. A1 - Baumann, Marcus T1 - The potential role of melted 'brown ice' as sources of chelators and ammonia to the surface waters of the Weddell Sea, Antarctica T2 - Proceedings of the NIPR Symposium on Polar Biology : 10 Y1 - 1997 SN - 0914-563X SP - 1 EP - 13 CY - Tokyo ER - TY - JOUR A1 - Baumann, Marcus T1 - Algenblüten & Treibhauseffekt : Wechselwirkungen mit anthropogenen Aktivitäten JF - Wechselwirkung : Wissenschaft & vernetztes Denken Y1 - 1996 SN - 0172-1623 VL - 18 IS - 81 SP - 6 EP - 11 ER - TY - JOUR A1 - Meiners, Klaus A1 - Baumann, Marcus T1 - Lokale Beiträge zum globalen Klimaschutz JF - Wechselwirkung : Wissenschaft & vernetztes Denken Y1 - 1997 SN - 0172-1623 VL - 19 IS - 84 SP - 6 EP - 12 ER - TY - THES A1 - Kobus, Timm T1 - Untersuchung des unterschiedlichen Reduktionsverhaltens von unterschiedlich para-substituierten Acetophenonen mit Chiralidon R & S T1 - Reductions of some para-substituted acetophenones with chiralidon-RS, correlation with the Hammett sigma Y1 - 2014 ER - TY - THES A1 - Maintz, Stephan T1 - Enantioselektive Reduktion prochiraler Carbonylverbindungen mit Chiralidon R & S in einem kontinuierlich betriebenen Festbettreaktor Y1 - 2014 ER - TY - PAT A1 - Bessler, Cornelius A1 - Evers, Stefan A1 - Maurer, Karl-Heinz A1 - Merkel, Marion A1 - Siegert, Petra A1 - Weber, Angrit A1 - Wieland, Susanne T1 - Leistungsverbesserte Proteasen und Wasch- und Reinigungsmittel enthaltend diese Proteasen [Offenlegungsschrift] T1 - Improved-performance proteases and detergents and cleaning agents comprising said proteases [Internationale Patentanmeldung] Y1 - 2009 SP - 1 EP - 41 PB - Deutsches Patent- und Markenamt / WIPO CY - München / Genf ER - TY - PAT A1 - Bessler, Cornelius A1 - Maurer, Karl-Heinz A1 - Merkel, Marion A1 - Siegert, Petra A1 - Wieland, Susanne T1 - Subtilisin from Bacillus Pumilus and detergent and cleaning agents containing said novel subtilisin [US Patentanmeldung / Internationale Patentanmeldung] Y1 - 2009 SP - 1 EP - 39 PB - USPTO; WIPO CY - Washington; Genf ER - TY - PAT A1 - Bessler, Cornelius A1 - Maurer, Karl-Heinz A1 - Merkel, Marion A1 - Siegert, Petra A1 - Wieland, Susanne T1 - Subtilisin aus Bacillus pumilus und Wasch- und Reinigungsmittel enthaltend dieses neue Subtilisin [Offenlegungsschrift] T1 - Subtilisin from Bacillus pumilus and washing and cleaning agents containing said novel subtilisin [Europäische Patentanmeldung / Internationale Patentanmeldung / Kanadische Patentanmeldung] Y1 - 2007 SP - 1 EP - 47 PB - Deutsches Patent- und Markenamt / Europäisches Patentamt / WIPO / CIPO CY - München / Den Hague / Genf ER - TY - JOUR A1 - Martinez, Ronny A1 - Jakob, Felix A1 - Tu, Ran A1 - Siegert, Petra A1 - Maurer, Karl-Heinz A1 - Schwaneberg, Ulrich T1 - Increasing activity and thermal resistance of Bacillus gibsonii alkaline protease (BgAP) by directed evolution JF - Biotechnology and bioengineering Y1 - 2013 SN - 1097-0290 (E-Journal); 0006-3592 (Print); 0368-1467 (Print) VL - Vol. 110 IS - Iss. 3 SP - 711 EP - 720 PB - Wiley CY - Weinheim ER - TY - JOUR A1 - Jakob, Felix A1 - Martinez, Ronny A1 - Mandawe, John A1 - Hellmuth, Hendrik A1 - Siegert, Petra A1 - Maurer, Karl-Heinz A1 - Schwaneberg, Ulrich T1 - Surface charge engineering of a Bacillus gibsonii subtilisin protease JF - Applied microbiology and biotechnology Y1 - 2013 SN - 1432-0614 (E-Journal); 0171-1741 (Print); 0175-7598 (Print); 0340-2118 (Print) VL - Vol. 97 IS - Iss. 15 SP - 6793 EP - 6802 PB - Springer CY - Berlin ER - TY - JOUR A1 - Niehaus, F. A1 - Gabor, E. A1 - Wieland, S. A1 - Siegert, Petra A1 - Maurer, Karl-Heinz A1 - Eck, J. T1 - Enzymes for the laundry industries: tapping the vast metagenomic pool of alkaline proteases JF - Microbial biotechnology Y1 - 2011 SN - 1432-0614 (E-Journal); 0171-1741 (Print); 0175-7598 (Print); 0340-2118 (Print) VL - Vol. 4 IS - Iss. 6 SP - 767 EP - 776 PB - Springer CY - Berlin ER - TY - JOUR A1 - Ribitsch, D. A1 - Heumann, S. A1 - Karl, W. A1 - Gerlach, J. A1 - Leber, R. A1 - Birner-Gruenberger, R. A1 - Gruber, K. A1 - Eiteljoerg, I. A1 - Remler, P. A1 - Siegert, Petra A1 - Lange, J. A1 - Maurer, Karl-Heinz A1 - Berg, G. A1 - Guebitz, G. M. A1 - Schwab, H. T1 - Extracellular serine proteases from Stenotrophomonas maltophilia: Screening, isolation and heterologous expression in E. coli JF - Journal of biotechnology N2 - A large strain collection comprising antagonistic bacteria was screened for novel detergent proteases. Several strains displayed protease activity on agar plates containing skim milk but were inactive in liquid media. Encapsulation of cells in alginate beads induced protease production. Stenotrophomonas maltophilia emerged as best performer under washing conditions. For identification of wash-active proteases, four extracellular serine proteases called StmPr1, StmPr2, StmPr3 and StmPr4 were cloned. StmPr2 and StmPr4 were sufficiently overexpressed in E. coli. Expression of StmPr1 and StmPr3 resulted in unprocessed, insoluble protein. Truncation of most of the C-terminal domain which has been identified by enzyme modeling succeeded in expression of soluble, active StmPr1 but failed in case of StmPr3. From laundry application tests StmPr2 turned out to be a highly wash-active protease at 45 °C. Specific activity of StmPr2 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 17 ± 2 U/mg. In addition we determined the kinetic parameters and cleavage preferences of protease StmPr2. KW - Alginate beads KW - Stenotrophomonas maltophilia KW - Detergent protease Y1 - 2012 U6 - http://dx.doi.org/10.1016/j.jbiotec.2011.09.025 SN - 1873-4863 (E-Journal); 0168-1656 (Print) VL - 157 IS - 1 SP - 140 EP - 147 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Ribitsch, D. A1 - Karl, W. A1 - Birner-Gruenberger, R. A1 - Gruber, K. A1 - Eiteljoerg, I. A1 - Remler, P. A1 - Wieland, S. A1 - Siegert, Petra A1 - Maurer, Karl-Heinz A1 - Schwab, H. T1 - C-terminal truncation of a metagenome-derived detergent protease for effective expression in E. coli JF - Journal of biotechnology N2 - Recently, a new alkaline protease named HP70 showing highest homology to extracellular serine proteases of Stenotrophomonas maltophilia and Xanthomonas campestris was found in the course of a metagenome screening for detergent proteases (Niehaus et al., submitted for publication). Attempts to efficiently express the enzyme in common expression hosts had failed. This study reports on the realization of overexpression in Escherichia coli after structural modification of HP70. Modelling of HP70 resulted in a two-domain structure, comprising the catalytic domain and a C-terminal domain which includes about 100 amino acids. On the basis of the modelled structure the enzyme was truncated by deletion of most of the C-terminal domain yielding HP70-C477. This structural modification allowed effective expression of active enzyme using E. coli BL21-Gold as the host. Specific activity of HP70-C477 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 30 ± 5 U/mg compared to 8 ± 1 U/mg of the native enzyme. HP70-C477 was most active at 40 °C and pH 7–11; these conditions are prerequisite for a potential application as detergent enzyme. Determination of kinetic parameters at 40 °C and pH = 9.5 resulted in KM = 0.23 ± 0.01 mM and kcat = 167.5 ± 3.6 s⁻¹. MS-analysis of peptide fragments obtained from incubation of HP70 and HP70-C477 with insulin B indicated that the C-terminal domain influences the cleavage preferences of the enzyme. Washing experiments confirmed the high potential of HP70-C477 as detergent protease. Y1 - 2010 U6 - http://dx.doi.org/10.1016/j.jbiotec.2010.09.947 SN - 1873-4863 (E-Journal); 0168-1656 (Print) VL - 150 IS - 3 SP - 408 EP - 416 PB - Elsevier CY - Amsterdam ER - TY - CHAP A1 - Wendorff, Marion A1 - Eggert, Thorsten A1 - Pohl, Martina A1 - Dresen, Carola A1 - Müller, Michael A1 - Jaeger, Karl-Erich A1 - Sprenger, Georg A. A1 - Schürmann, Melanie A1 - Schürmann, Martin A1 - Johnen, Sandra A1 - Sprenger, Gerda A1 - Sahm, Hermann A1 - Inoue, Tomoyuki A1 - Schörken, Ulrich A1 - Breittaupt, Holger A1 - Frölich, Bettina A1 - Heim, Petra A1 - Iding, Hans A1 - Juchem, Bettina A1 - Siegert, Petra A1 - Kula, Maria-Regina A1 - Weckbecker, Andrea A1 - Hummel, Werner A1 - Fessner, Wolf-Dieter A1 - Elling, Lothar A1 - Wolberg, Michael A1 - Bode, Silke A1 - Feldmann, Ralf A1 - Geilenkirchen, Petra A1 - Schubert, Thomas A1 - Walter, Lydia A1 - Dünnwald, Thomas A1 - Demir, Ayhan S. A1 - Kolter-Jung, Doris A1 - Nitsche, Adam A1 - Dünkelmann, Pascal A1 - Cosp, Annabel A1 - Lingen, Bettina T1 - Catalytic asymmetric synthesis : section 2.2 T2 - Asymmetric synthesis with chemical and biological methods / ed. by Dieter Enders ... Y1 - 2007 SN - 978-3-527-31473-7 SP - 298 EP - 413 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Siegert, Petra A1 - McLeish, Michael J. A1 - Baumann, Martin A1 - Iding, Hans A1 - Kneen, Malea M. A1 - Kenyon, George L. A1 - Pohl, Martina T1 - Exchanging the substrate specificities of pyruvate decarboxylase from Zymomonas mobilis and benzoylformate decarboxylase from Pseudomonas putida JF - Protein engineering, design, and selection : peds Y1 - 2005 SN - 1460-213X (E-Journal); 1741-0134 (E-Journal); 0269-2139 (Print); 1741-0126 (Print) VL - Vol. 18 IS - Iss. 7 SP - 345 EP - 357 ER - TY - PAT A1 - Siegert, Petra A1 - Merkel, Marion A1 - Kluin, Cornelia A1 - O'Connell, Timothy A1 - Maurer, Karl-Heinz T1 - Wasch- und Reinigungsmittel enthaltend Proteasen aus Xanthomonas [Offenlegungsschrift] T1 - Detergents and cleaners comprising proteases from xanthomonas [Internationale Patentanmeldung] Y1 - 2008 SP - 1 EP - 15 PB - Deutsches Patent- und Markenamt / WIPO CY - München / Genf ER - TY - THES A1 - Huber, Eugen T1 - Selektive Reduktion von bifunktionellen aromatischen Carbonylverbindungen Y1 - 2014 PB - FH Aachen CY - Aachen ER - TY - CHAP A1 - Siegert, Petra A1 - Pohl, Martina A1 - Kneen, Malea M. A1 - Pogozheva, Irina D. A1 - Kenyon, George L. A1 - McLeish, Michael J. T1 - Exploring the substrate specificity of benzoylformate decarboxylase, pyruvate decarboxylase, and benzaldehyde lyase T2 - Thiamine : catalytic mechanisms in normal and disease states / ed. by Frank Jordan ... Y1 - 2004 SN - 0-8247-4062-9 SP - 275 EP - 290 PB - Dekker CY - New York, NY ER - TY - JOUR A1 - Dünkelmann, Pascal A1 - Kolter-Jung, Doris A1 - Nitsche, Adam A1 - Demir, Ayhan S. A1 - Siegert, Petra A1 - Lingen, Bettina A1 - Baumann, Martin A1 - Pohl, Martina A1 - Müller, Michael T1 - Development of a donor-acceptor concept for enzymatic cross-coupling reactions of adehydes : the first asymmetric cross-benzoin condensation JF - Journal of the American Chemical Society Y1 - 2002 SN - 1520-5126 (E-Journal); 0002-7863 (Print) VL - Vol. 124 SP - 12084 EP - 12085 ER - TY - JOUR A1 - Dünnwald, Thomas A1 - Demir, Ayhan S. A1 - Siegert, Petra A1 - Pohl, Martina A1 - Müller, Michael T1 - ChemInform Abstract: Enantioselective synthesis of (S)-2-Hydroxypropanone derivatives by Benzoylformate Decarboxylase Catalyzed C—C Bond Formation JF - Cheminform Y1 - 2001 SN - 1522-2667 (E-Journal); 0931-7597 (Print) VL - Vol. 32 IS - Iss. 4 SP - Publ. online ER - TY - JOUR A1 - Dünnwald, Thomas A1 - Demir, Ayhan S. A1 - Siegert, Petra A1 - Pohl, Martina A1 - Müller, Michael T1 - Enantioselective Synthesis of (S)-2-Hydroxypropanone Derivatives by Benzoylformate Decarboxylase Catalyzed C−C Bond Formation JF - European journal of organic chemistry Y1 - 2000 SN - 0365-5490 (E-Journal); 1099-0690 (E-Journal); 0075-4617 (Print); 0170-2041 (Print); 0947-3440 (Print); 1434-193X (Print); 1434-243X (Print) VL - Vol. 2000 IS - Iss. 11 SP - 2161 EP - 2170 ER - TY - CHAP A1 - Siegert, Petra A1 - Iding, Hans A1 - Baumann, Martin A1 - McLeish, Michael J. A1 - Kenyon, George L. A1 - Pohl, Martina T1 - Broadening of the substrate spectra of two ThDP-dependent decarboxylases using site-directed-mutagenesis T2 - Proceedings of the 4th International Congress on Biochemical Engineering : 17 and 18 February 2000, Stuttgart Y1 - 2000 SN - 3-8167-5570-4 SP - 38 EP - 42 ER - TY - JOUR A1 - Iding, Hans A1 - Dünnwald, Thomas A1 - Greiner, Lasse A1 - Liese, Andreas A1 - Müller, Michael A1 - Siegert, Petra A1 - Grötzinger, Joachim A1 - Demir, Ayhan S. A1 - Pohl, Martina T1 - Benzoylformate Decarboxylase from Pseudomonas putida as Stable Catalyst for the Synthesis of Chiral 2-Hydroxy Ketones JF - Chemistry - a European journal Y1 - 2000 SN - 1521-3765 (E-Journal); 0947-6539 (Print) VL - Vol. 6 IS - Iss. 8 SP - 1483 EP - 1495 ER - TY - JOUR A1 - Iding, Hans A1 - Siegert, Petra A1 - Mesch, K. A1 - Pohl, Martina T1 - Application of α-keto acid decarboxylases in biotransformations JF - Biochimica et biophysica acta (BBA) - Protein structure and molecular enzymology Y1 - 1998 SN - 1879-2588 (E-Journal); 0167-4838 (Print) VL - Vol. 1385 IS - Iss. 2 SP - 307 EP - 322 ER - TY - JOUR A1 - Pohl, Martina A1 - Siegert, Petra A1 - Mesch, K. A1 - Bruhn, H. A1 - Grötzinger, Joachim T1 - Active site mutants of pyruvate decarboxylase from Zymomonas mobilis : a site-directed mutagenesis study of L112, I472, I476, E473 and N482 JF - European journal of biochemistry Y1 - 1998 SN - 1432-1033 (E-Journal); 1742-4658 (E-Journal); 0014-2956 (Print); 1742-464X (Print) VL - Vol. 257 IS - Iss. 3 SP - 538 EP - 546 ER - TY - PAT A1 - Siegert, Petra A1 - Evers, Stefan A1 - Marion, Merkel A1 - Mussmann, Nina A1 - Hellmuth, Hendrik A1 - O'Connell, Timothy A1 - Maurer, Karl-Heinz A1 - Schwaneberg, Ulrich A1 - Martinez, Ronny A1 - Jakob, Felix T1 - Verfahren zur Anpassung eines hydrolytischen Enzyms an eine das hydrolytische Enzym stabilisierende Komponente [Offenlegungsschrift] T1 - Method for adapting a hydrolytic enzyme to a component that stabilizes the hydrolytic enzyme [Europäische Patentanmeldung / Internationale Patentanmeldung] Y1 - 2013 SP - 1 EP - 27 PB - Deutsches Patent- und Markenamt / Europäisches Patentamt / WIPO CY - München / Den Hague / Genf ER - TY - PAT A1 - Maurer, Karl-Heinz A1 - O'Connell, Timothy A1 - Siegert, Petra A1 - Weber, Thomas A1 - Tondera, Susanne A1 - Hellmuth, Hendrik T1 - Flüssige Tensidzubereitung enthaltend Lipase und Phosphonat [Offenlegungsschrift] T1 - Liquid surfactant preparation containing lipase and phosphonate [Europäische Patentanmeldung / Internationale Patentanmeldung] Y1 - 2012 SP - 1 EP - 22 PB - Deutsches Patent- und Markenamt / Europäisches Patentamt / WIPO CY - München / Den Hague / Genf ER - TY - PAT A1 - Siegert, Petra A1 - Merkel, Marion A1 - Hellmuth, Hendrik A1 - O'Connell, Timothy A1 - Maurer, Karl-Heinz T1 - Stabilisierte flüssige enzymhaltige Tensidzubereitung (Einsatz einer das hydrolytische Enzym stabilisierenden Komponente, die eine Phenylalkyldicarbonsäure umfasst) [Offenlegungsschrift] T1 - Stabilized liquid tenside preparation comprising enzymes (using a component that stabilizes the hydrolytic enzyme and includes a phenylalkyldicarboxylic acid) [Europäische Patentanmeldung / Internationale Patentanmeldung] Y1 - 2012 SP - 1 EP - 15 PB - Deutsches Patent- und Markenamt / Europäisches Patentamt / WIPO CY - München / Den Hague / Genf ER - TY - PAT A1 - Siegert, Petra A1 - Merkel, Marion A1 - Hellmuth, Hendrik A1 - O'Connell, Timothy A1 - Maurer, Karl-Heinz T1 - Stabilisierte flüssige enzymhaltige Tensidzubereitung (durch den Einsatz einer das hydrolytische Enzym stabilisierende Komponente, die eine mehrfach substituierte Benzolcarbonsäure umfasst, die an mindestens zwei Kohlenstoffatomen des Benzolrestes eine Carboxylgruppe aufweist) [Offenlegungsschrift] T1 - Stabilized liquid tenside preparation comprising enzymes (a component comprising a multiply substituted benzyl carboxylic acid comprising a carboxyl group on at least two carbon atoms of the benzyl radical is used for stabilizing the hydrolytic enzyme) [Europäische Patentanmeldung / Internationale Patentanmeldung] Y1 - 2012 SP - 1 EP - 16 PB - Deutsches Patent- und Markenamt / Europäisches Patentamt / WIPO CY - München / Den Hague / Genf ER - TY - PAT A1 - Siegert, Petra A1 - Merkel, Marion A1 - Hellmuth, Hendrik A1 - O'Connell, Timothy A1 - Maurer, Karl-Heinz T1 - Stabilisierte flüssige enzymhaltige Tensidzubereitung (Einsatz einer das hydrolytische Enzym stabilisierende Komponente, die eine Phthaloylglutaminsäure und/oder eine Phthaloylasparaginsäure umfasst) [Offenlegungsschrift] T1 - Stabilized liquid tenside preparation comprising enzymes (a component comprising a phthaloyl glutamine acid and/or a phthaloyl asparagine acid is used for stablizing the hydrolytic enzyme) [Europäische Patentanmeldung / Internationale Patentanmeldung] Y1 - 2012 SP - 1 EP - 16 PB - Deutsches Patent- und Markenamt / Europäisches Patentamt / WIPO CY - München / Den Hague / Genf ER - TY - PAT A1 - Siegert, Petra A1 - Merkel, Marion A1 - Hellmuth, Hendrik A1 - O'Connell, Timothy A1 - Maurer, Karl-Heinz T1 - Stabilisierte flüssige enzymhaltige Tensidzubereitung (Einsatz einer das hydrolytische Enzym stabilisierende Komponente, die eine Aminophthalsäure umfasst) [Offenlegungsschrift] T1 - Stabilized liquid tenside preparation comprising enzymes (a component comprising an aminophthalic acid is used for stabilizing the hydrolytic enzyme) [Europäische Patentanmeldung / Internationale Patentanmeldung] Y1 - 2012 SP - 1 EP - 16 PB - Deutsches Patent- und Markenamt / Europäisches Patentamt / WIPO CY - München / Den Hague / Genf ER - TY - PAT A1 - Siegert, Petra A1 - Merkel, Marion A1 - Hellmuth, Hendrik A1 - O'Connell, Timothy A1 - Maurer, Karl-Heinz T1 - Stabilisierte flüssige enzymhaltige Tensidzubereitung (Einsatz einer das hydrolytische Enzym stabilisierende Komponente, die eine Oligoamino-biphenyl-oligocarbonsäure umfasst) [Offenlegungsschrift] T1 - Stabilized liquid tenside preparation comprising enzymes (a component comprising an oligoamino-biphenyl-oligocarboxylic acid is used for stabilizing the hydrolytic enzyme) [Europäische Patentanmeldung / Internationale Patentanmeldung] Y1 - 2012 SP - 1 EP - 16 PB - Deutsches Patent- und Markenamt / Europäisches Patentamt / WIPO CY - München / Den Hague / Genf ER - TY - PAT A1 - Siegert, Petra A1 - Merkel, Marion A1 - Hellmuth, Hendrik A1 - O'Connell, Timothy A1 - Maurer, Karl-Heinz T1 - Stabilisierte flüssige enzymhaltige Tensidzubereitung (Einsatz einer das hydrolytische Enzym stabilisierende Komponente, die ein Monosaccharidglycerat umfasst) [Offenlegungsschrift] T1 - Stabilized liquid enzyme-containing surfactant preparation (using a component which comprises a monosaccharide glycerate and which stabilizes the hydrolytic enzyme) [Europäische Patentanmeldung / Internationale Patentanmeldung] Y1 - 2012 SP - 1 EP - 17 PB - Deutsches Patent- und Markenamt / Europäisches Patentamt / WIPO CY - München / Den Hague / Genf ER - TY - PAT A1 - Siegert, Petra A1 - Merkel, Marion A1 - Kluin, Cornelia A1 - Maurer, Karl-Heinz A1 - O'Connell, Timothy A1 - Wieland, Susanne A1 - Hellmuth, Hendrik T1 - Neue Proteasen und diese enthaltende Mittel [Offenlegungsschrift] T1 - Novel proteases and compositions comprising them [Europäische Patentanmeldung / Internationale Patentanmeldung] Y1 - 2011 SP - 1 EP - 21 PB - Deutsches Patent- und Markenamt / Europäisches Patentamt / WIPO CY - München / Den Hague / Genf ER - TY - PAT A1 - Siegert, Petra A1 - Schwaneberg, Ulrich A1 - Martinez Moya, Ronny A1 - Merkel, Marion A1 - Spitz, Astrid A1 - Wieland, Susanne A1 - Hellmuth, Hendrik A1 - Maurer, Karl-Heinz T1 - Leistungsverbesserte Proteasevariante [Offenlegungsschrift] T1 - Performance-enhanced protease variant [Europäische Patentanmeldung / Internationale Patentanmeldung] Y1 - 2012 SP - 1 EP - 29 PB - Deutsches Patent- und Markenamt / Europäisches Patentamt / WIPO CY - München / Den Hague / Genf ER - TY - PAT A1 - Wieland, Susanne A1 - Siegert, Petra A1 - Spitz, Astrid A1 - Maurer, Karl-Heinz A1 - O'Connell, Timothy A1 - Prüser, Inken A1 - Schiedel, Marc-Steffen A1 - Eiting, Thomas A1 - Sendor-Müller, Dorota A1 - Bastigkeit, Thorsten A1 - Benda, Konstantin A1 - Müller, Sven T1 - Lagerstabiles flüssiges Wasch- oder Reinigungsmittel enthaltend Proteasen [Offenlegungsschrift] T1 - Storage-stable liquid washing or cleaning agent containing proteases [Europäische Patentanmeldung / Internationale Patentanmeldung] Y1 - 2011 SP - 1 EP - 25 PB - Deutsches Patent- und Markenamt / Europäisches Patentamt / WIPO CY - München / Den Hague / Genf ER - TY - PAT A1 - Siegert, Petra A1 - Mussmann, Nina A1 - O'Connell, Timothy A1 - Maurer, Karl-Heinz T1 - Neue Proteasen und Mittel enthaltend diese Proteasen [Offenlegungsschrift] T1 - Novel proteases and means containing said proteases [Internationale Patentanmeldung] Y1 - 2010 SP - 1 EP - 30 PB - Deutsches Patent- und Markenamt / WIPO CY - München / Genf ER - TY - PAT A1 - Siegert, Petra A1 - Baumstark, Rebecca A1 - Kluin, Cornelia A1 - O'Connell, Timothy A1 - Maurer, Karl-Heinz A1 - Hellmuth, Hendrik T1 - Neue Proteasen und Mittel enthaltend diese Proteasen [Offenlegungsschrift] Y1 - 2010 SP - 1 EP - 30 PB - Deutsches Patent- und Markenamt CY - München ER - TY - PAT A1 - Siegert, Petra A1 - Spitz, Astrid A1 - Maurer, Karl-Heinz T1 - Neue Proteasen und Mittel enthaltend diese Proteasen [Offenlegungsschrift] T1 - Novel proteases and compositions comprising these proteases [Internationale Patentanmeldung] Y1 - 2010 SP - 1 EP - 31 PB - Deutsches Patentamt / WIPO CY - München / Genf ER - TY - PAT A1 - Siegert, Petra A1 - Spitz, Astrid A1 - Maurer, Karl-Heinz T1 - Wasch- und Reinigungsmittel enthaltend Proteasen aus Bacillus pumilus [Offenlegungsschrift] T1 - Detergents and cleaning agents containing proteases from bacillus pumilus [Europäische Patentschrift / Internationale Patentanmeldung] Y1 - 2010 SP - 1 EP - 20 PB - Deutsches Patentamt / Europäisches Patentamt / WIPO CY - München / Den Hague / Genf ER - TY - PAT A1 - O'Connell, Timothy A1 - Siegert, Petra A1 - Evers, Stefan A1 - Bongaerts, Johannes A1 - Weber, Thomas A1 - Maurer, Karl-Heinz A1 - Bessler, Cornelius T1 - Wasch- oder Reinigungsmittel mit gesteigerter Waschkraft [Offenlegungsschrift] T1 - Method from improving the cleaning action of a detergent of cleaning agent [US Patentanmeldung] Y1 - 2010 SP - 1 EP - 34 PB - Deutsches Patentamt CY - München ER - TY - PAT A1 - Berg, Gabriele A1 - Bessler, Cornelius A1 - Gerlach, Jochen A1 - Gübitz, Georg A1 - Heumann, Sonja A1 - Karl, Wolfgang A1 - Maurer, Karl-Heinz A1 - Remler, Peter A1 - Ribitsch, Doris A1 - Schwab, Helmut A1 - Siegert, Petra A1 - Wieland, Susanne T1 - Mittel enthaltend Proteasen aus Stenotrophomonas maltophilia [Offenlegungsschrift] T1 - Agents containing proteases from Stenotrophomonas maltophilia [Europäische Patentanmeldung / US Patentanmeldung / Internationale Patentanmeldung] Y1 - 2009 SP - 1 EP - 47 PB - Deutsches Patent- und Markenamt / Europäisches Patentamt / USPTO / WIPO CY - München / Den Hague / Washington / Genf ER - TY - PAT A1 - Siegert, Petra A1 - Wieland, Susanne A1 - Engelskirchen, Julia A1 - Merkel, Marion A1 - Maurer, Karl-Heinz A1 - Bessler, Cornelius T1 - Neue Alkalische Protease aus Bacillus gibsonii und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease [Offenlegungsschrift] T1 - Novel alkaline protease from Bacillus Gibsonii and washing and cleaning agents containing said novel alkaline protease [Europäische Patentanmeldung / US Patentanmeldung / Internationale Patentanmeldung] Y1 - 2008 SP - 1 EP - 51 PB - Deutsches Patentamt / Europäisches Patentamt / WIPO CY - München / Den Hague / Genf ER - TY - PAT A1 - Merkel, Marion A1 - Weber, Angrit A1 - Siegert, Petra A1 - Wieland, Susanne A1 - Maurer, Karl-Heinz A1 - Bessler, Cornelius T1 - Neue Alkalische Protease aus Bacillus gibsonii und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease [Offenlegungsschrift] T1 - Novel alkaline protease from Bacillus Gibsonii and washing and cleaning agents containing said novel alkaline protease [Internationale Patentanmeldung] Y1 - 2007 SP - 1 EP - 46 PB - Deutsches Patent- und Markenamt / WIPO CY - München / Genf ER - TY - PAT A1 - O'Connell, Timothy A1 - Hoven, Nina A1 - Siegert, Petra A1 - Maurer, Karl-Heinz T1 - Amadoriasen in Wasch- und Reinigungsmitteln [Offenlegungsschrift] T1 - Amadoriases in washing and cleaning products [Europäische Patentanmeldung / Internationale Patentanmeldung] Y1 - 2007 SP - 1 EP - 45 PB - Deutsches Patent- und Markenamt / Europäisches Patentamt / WIPO CY - München / Den Hague / Genf ER - TY - PAT A1 - Schulze zur Wiesche, Erik A1 - Otto, Ralf A1 - Kleen, Astrid A1 - Hollenberg, Detlef A1 - Sättler, Andrea A1 - Siegert, Petra A1 - Boßmann, Britta T1 - Verfahren zur dauerhaften Ausrüstung keratinischer Fasern mit Pflegewirkstoffen durch Carboxylesterhydrolasen [Offenlegungsschrift] T1 - Method for the permanent deposition of conditioning agents on keratinic fibres using carboxylester hydrolases [Europäische Patentanmeldung] Y1 - 2005 SP - 1 EP - 13 PB - Deutsches Patent- und Markenamt / Europäisches Patentamt CY - München / Den Hague ER - TY - PAT A1 - Kluin, Cornelia A1 - Maurer, Karl-Heinz A1 - Banowski, Bernhard A1 - Bessler, Cornelius A1 - Siegert, Petra T1 - Verwendung von Siderophoren gegen Geruchskeime [Offenlegungsschrift] T1 - Use of siderophores for control of odor-producing bacteria, particularly for treating or preventing body odor, act by depriving bacteria of iron Y1 - 2005 SP - 1 EP - 32 PB - Deutsches Patent- und Markenamt CY - München ER - TY - PAT A1 - Bankowski, Bernhard A1 - Hoffmann, Daniele A1 - Wadle, Armin A1 - Siegert, Petra A1 - Sättler, Andrea A1 - Gerke, Thomas T1 - Beta-Glucuronidase-Inhibitoren in Deodorantien und Antitranspirantien [Offenlegungsschrift] T1 - Beta-Glucuronidase inhibitors for use in deodorants and antiperspirants [Europäische Patentanmeldung / Internationale Patentanmeldung] Y1 - 2003 SP - 1 EP - 24 PB - Deutsches Patent- und Markenamt / Europäisches Patentamt / WIPO CY - München / Den Hague / Genf ER - TY - PAT A1 - Siegert, Petra A1 - Bankowski, Bernhard T1 - Verwendung von Steroidsulfatase-Inhibitoren zur Verminderung von Haarausfall ; Kosmetische und pharmazeutische Zusammensetzungen enthaltend Steroidsulfatase-Inhibitoren und deren Verwendung zur Verminderung von Haarausfall [Offenlegungsschrift] T1 - Cosmetic and pharmaceutical compositions comprising steroid sulfatase inhibitors and their use for the reduction of hair loss [Europäische Patentanmeldung] Y1 - 2003 SP - 1 EP - 38 PB - Deutsches Patent- und Markenamt / Europäisches Patentamt CY - München / Den Hague ER - TY - PAT A1 - Banowski, Bernhard A1 - Wadle, Armin A1 - Siegert, Petra T1 - Arylsulfatase-Inhibitoren in Deodorantien und Antitranspirantien [Offenlegungsschrift] T1 - Arylsulfatase inhibitors in deodorants and antiperspirants [Europäische Patentanmeldung] Y1 - 2004 SP - 1 EP - 15 PB - Deutsches Patent- und Markenamt / Europäisches Patentamt CY - München / Den Hague ER - TY - PAT A1 - Banowski, Bernhard A1 - Wadle, Armin A1 - Siegert, Petra T1 - Arylsulfatase-Inhibitoren in Deodorantien und Antitranspirantien [Offenlegungsschrift] T1 - Reducing body odor, e.g. in men, comprises applying deodorant or antiperspirant composition containing arylsulfatase-inhibiting phenoxyphenol derivative to skin Y1 - 2003 SP - 1 EP - 20 PB - Deutsches Patent- und Markenamt CY - München ER - TY - PAT A1 - Banowski, Bernhard A1 - Hoffmann, Daniele A1 - Wadle, Armin A1 - Siegert, Petra A1 - Sättler, Andrea A1 - Gerke, Thomas T1 - Arylsulfatase-Inhibitoren in Deodorantien und Antitranspirantien [Offenlegungsschrift] T1 - Arylsufatas-inhibitors in deodorants and antiperspirants[Europäische Patentschrift / Österreichische Patentanmeldung / Polnische Patentanmeldung / Internationale Patentanmeldung] Y1 - 2002 SP - 1 EP - 22 PB - Deutsches Patent- und Markenamt / Polish Patent Office / WIPO CY - München / Warsaw / Genf ER - TY - PAT A1 - Banowski, Bernhard A1 - Wadle, Armin A1 - Siegert, Petra T1 - Use of hydroxydiphenyl ether derivatives as arylsulfatase-inhibitors in deodorants and antiperspirants [Europäische Patentanmeldung / US Patentanmeldung / Australische Patentanmeldung / Internationale Patentanmeldung] T1 - Verwendung von Hydroxydiphenylether-Derivaten als Arylsulfatase-Hemmer in Deodorants und Antitranspirantien Y1 - 2005 SP - 1 EP - 40 PB - Europäisches Patentamt / USPTO / IP Australia / WIPO CY - Den Hague / Washington / Canberra/ Genf ER - TY - PAT A1 - Schmitz, Susanne A1 - Spitz, Astrid A1 - Merkel, Marion A1 - Siegert, Petra T1 - Verfahren zur Bestimmung des enzymatischen Status humaner Haut in vitro [Offenlegungsschrift] T1 - Method for determining the enzymatic status of human skin in vitro [Europäische Patentanmeldung / Australische Patentanmeldung / Internationale Patentanmeldung] Y1 - 2004 SP - 1 EP - 7 PB - Deutsches Patent- und Markenamt / Europäisches Patentamt / IP Australia / WIPO CY - München / Den Hague / Canberra / Genf ER - TY - PAT A1 - Banowski, Bernhard A1 - Wadle, Armin A1 - Siegert, Petra A1 - Sättler, Andrea T1 - Lipase-Inhibitoren in Deodorantien und Antitranspirantien [Offenlegungsschrift] T1 - Lipase inhibitors in deodorants and antiperspirants [Europäische Patentanmeldung] Y1 - 2004 SP - 1 EP - 22 PB - Deutsches Patent- und Markenamt / Europäisches Patentamt CY - München / Den Hague ER - TY - PAT A1 - Banowski, Bernhard A1 - Waldmann-Laue, Marianne A1 - Wadle, Armin A1 - Siegert, Petra A1 - Sättler, Andreas T1 - 5-Lipoxigenase-Inhibitoren in Deodorantien und Antitranspirantien [Offenlegungsschrift] T1 - 5-Lipooxygenase inhibitors in deodorants and antiperspirants [Europäische Patentanmeldung] Y1 - 2004 SP - 1 EP - 12 PB - Deutsches Patent- und Markenamt / Europäisches Patentamt CY - München / Den Hague ER - TY - JOUR A1 - Ratke, Lorenz A1 - Milow, Barbara A1 - Lisinski, Susanne A1 - Hoepfner, Sandra T1 - On an effect of fine ceramic particles on the structure of aerogels JF - Microgravity science and technology Y1 - 2014 U6 - http://dx.doi.org/10.1007/s12217-014-9380-2 SN - 0938-0108 ; 1875-0494 VL - 26 SP - 103 EP - 110 PB - Springer Nature CY - Heidelberg ER - TY - JOUR A1 - Handtke, Stefan A1 - Volland, Sonja A1 - Methling, Karen A1 - Albrecht, Dirk A1 - Becher, Dörte A1 - Nehls, Jenny A1 - Bongaerts, Johannes A1 - Maurer, Karl-Heinz A1 - Lalk, Michael A1 - Liesegang, Heiko A1 - Voigt, Birgit A1 - Daniel, Rolf A1 - Hecker, Michael T1 - Cell physiology of the biotechnological relevant bacterium Bacillus pumilus - An omics-based approach JF - Journal of Biotechnology N2 - Members of the species Bacillus pumilus get more and more in focus of the biotechnological industry as potential new production strains. Based on exoproteome analysis, B. pumilus strain Jo2, possessing a high secretion capability, was chosen for an omics-based investigation. The proteome and metabolome of B. pumilus cells growing either in minimal or complex medium was analyzed. In total, 1542 proteins were identified in growing B. pumilus cells, among them 1182 cytosolic proteins, 297 membrane and lipoproteins and 63 secreted proteins. This accounts for about 43% of the 3616 proteins encoded in the B. pumilus Jo2 genome sequence. By using GC–MS, IP-LC/MS and H NMR methods numerous metabolites were analyzed and assigned to reconstructed metabolic pathways. In the genome sequence a functional secretion system including the components of the Sec- and Tat-secretion machinery was found. Analysis of the exoproteome revealed secretion of about 70 proteins with predicted secretion signals. In addition, selected production-relevant genome features such as restriction modification systems and NRPS clusters of B. pumilus Jo2 are discussed. Y1 - 2014 U6 - http://dx.doi.org/10.1016/j.jbiotec.2014.08.028 SN - 1873-4863 (E-Journal); 0168-1656 (Print) IS - 192(A) SP - 204 EP - 214 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Nachtrodt, Frederik A1 - Tietsch, Wolfgang A1 - Mostacci, Domiziano A1 - Scherer, Ulrich W. T1 - Set-up and first operation of a plasma oven for treatment of low level radioactive wastes JF - Nuclear technology and radiation protection Y1 - 2014 U6 - http://dx.doi.org/10.2298/NTRP140SS47N SN - 1451-3994 VL - 29 IS - Suppl. SP - 47 EP - 51 PB - VINČA Institute of Nuclear Sciences CY - Belgrad ER - TY - CHAP A1 - Frotscher, Ralf A1 - Goßmann, Matthias A1 - Raatschen, Hans-Jürgen A1 - Temiz Artmann, Aysegül A1 - Staat, Manfred T1 - Simulation of cardiac cell-seeded membranes using the edge-based smoothed FEM T2 - Shell and membrane theories in mechanics and biology. (Advanced structured materials ; 45) N2 - We present an electromechanically coupled Finite Element model for cardiac tissue. It bases on the mechanical model for cardiac tissue of Hunter et al. that we couple to the McAllister-Noble-Tsien electrophysiological model of purkinje fibre cells. The corresponding system of ordinary differential equations is implemented on the level of the constitutive equations in a geometrically and physically nonlinear version of the so-called edge-based smoothed FEM for plates. Mechanical material parameters are determined from our own pressure-deflection experimental setup. The main purpose of the model is to further examine the experimental results not only on mechanical but also on electrophysiological level down to ion channel gates. Moreover, we present first drug treatment simulations and validate the model with respect to the experiments. Y1 - 2015 SN - 978-3-319-02534-6 ; 978-3-319-02535-3 SP - 187 EP - 212 PB - Springer CY - Heidelberg ER - TY - JOUR A1 - Raue, Markus A1 - Wambach, M. A1 - Glöggler, S. A1 - Grefen, Dana A1 - Kaufmann, R. A1 - Abetz, C. A1 - Georgopanos, P. A1 - Handge, U. A. A1 - Mang, Thomas A1 - Blümich, B. A1 - Abetz, V. T1 - Investigation of historical hard rubber ornaments of Charles Goodyear JF - Macromolecular chemistry and physics Y1 - 2014 SN - 1022-1352 VL - Vol. 215 IS - No. 3 SP - 245 EP - 254 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Seifarth, Volker A1 - Goßmann, Matthias A1 - Grosse, J. O. A1 - Becker, C. A1 - Heschel, I. A1 - Artmann, Gerhard A1 - Temiz Artmann, Aysegül T1 - Development of a Bioreactor to Culture Tissue Engineered Ureters Based on the Application of Tubular OPTIMAIX 3D Scaffolds JF - Urologia Internationalis Y1 - 2015 U6 - http://dx.doi.org/10.1159/000368419 SN - 0042-1138 VL - 2015 IS - 95 SP - 106 EP - 113 PB - Karger CY - Basel ER - TY - JOUR A1 - Kusch, Peter A1 - Rieser, Claudia A1 - Knupp, Gerd A1 - Mang, Thomas T1 - Characterization of copolymers of methacrylic acid with poly(ethylene glycol) methyl ether methacrylate macromonomers by analytical pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS) JF - Journal of Analytical and Applied Pyrolysis Y1 - 2015 U6 - http://dx.doi.org/10.1016/j.jaap.2015.03.003 SN - 0165-2370 VL - Vol. 113 SP - 412 EP - 418 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Pilas, Johanna A1 - Iken, Heiko A1 - Selmer, Thorsten A1 - Keusgen, Michael A1 - Schöning, Michael Josef T1 - Development of a multi‐parameter sensor chip for the simultaneous detection of organic compounds in biogas processes JF - Physica status solidi (a) N2 - An enzyme-based multi-parameter biosensor is developed for monitoring the concentration of formate, d-lactate, and l-lactate in biological samples. The sensor is based on the specific dehydrogenation by an oxidized β-nicotinamide adenine dinucleotide (NAD+)-dependent dehydrogenase (formate dehydrogenase, d-lactic dehydrogenase, and l-lactic dehydrogenase, respectively) in combination with a diaphorase from Clostridium kluyveri (EC 1.8.1.4). The enzymes are immobilized on a platinum working electrode by cross-linking with glutaraldehyde (GA). The principle of the determination scheme in case of l-lactate is as follows: l-lactic dehydrogenase (l-LDH) converts l-lactate into pyruvate by reaction with NAD+. In the presence of hexacyanoferrate(III), the resulting reduced β-nicotinamide adenine dinucleotide (NADH) is then regenerated enzymatically by diaphorase. The electrochemical detection is based on the current generated by oxidation of hexacyanoferrate(II) at an applied potential of +0.3 V vs. an Ag/AgCl reference electrode. The biosensor will be electrochemically characterized in terms of linear working range and sensitivity. Additionally, the successful practical application of the sensor is demonstrated in an extract from maize silage. Y1 - 2015 U6 - http://dx.doi.org/10.1002/pssa.201431894 SN - 1862-6319 VL - 212 IS - 6 SP - 1306 EP - 1312 PB - Wiley CY - Weinheim ER - TY - JOUR A1 - Breuer, Lars A1 - Raue, Markus A1 - Kirschbaum, M. A1 - Mang, Thomas A1 - Schöning, Michael Josef A1 - Thoelen, R. A1 - Wagner, Torsten T1 - Light-controllable polymeric material based on temperature-sensitive hydrogels with incorporated graphene oxide JF - Physica status solidi (a) N2 - Poly(N-isopropylacrylamide) (PNIPAAm) hydrogel films with incorporated graphene oxide (GO) were developed and tested as light-stimulated actuators. GO dispersions were synthesized via Hummers method and characterized toward their optical properties and photothermal energy conversion. The hydrogels were prepared by means of photopolymerization. In addition, the influence of GO within the hydrogel network on the lower critical solution temperature (LCST) was investigated by differential scanning calorimetry (DSC). The optical absorbance and the response to illumination were determined as a function of GO concentration for thin hydrogel films. A proof of principle for the stimulation with light was performed. Y1 - 2015 U6 - http://dx.doi.org/10.1002/pssa.201431944 SN - 1862-6319 VL - 212 IS - 6 SP - 1368 EP - 1374 PB - Wiley CY - Weinheim ER - TY - JOUR A1 - Raab, Monika A1 - Kappel, Sven A1 - Krämer, Andrea A1 - Sanhaji, Mourad A1 - Matthess, Yves A1 - Kurunci-Csacsko, Elisabeth A1 - Calzada-Wack, Julia A1 - Rathkolb, Birgit A1 - Rosman, Jan A1 - Adler, Thure A1 - Busch, Dirk H. A1 - Esposito, Irene A1 - Fuchs, Helmut A1 - Gailus-Durner, Valérie A1 - Klingenspor, Martin A1 - Wolf, Eckhard A1 - Sänger, Nicole A1 - Prinz, Florian A1 - Hrabe de Angelis, Martin A1 - Seibler, Jost A1 - Yuan, Juping A1 - Bergmann, Martin A1 - Knecht, Rainald A1 - Kreft, Bertolt A1 - Strebhardt, Klaus T1 - Toxicity modelling of Plk1-targeted therapies in genetically engineered mice and cultured primary mammalian cells JF - Nature Communications Y1 - 2011 U6 - http://dx.doi.org/10.1038/ncomms1395 SN - 2041-1723 VL - 2 IS - 395 SP - 1 EP - 11 PB - Nature CY - London ER - TY - JOUR A1 - Hasegawa, Maki A1 - Kapelyukh, Yury A1 - Tahara, Harunobu A1 - Seibler, Jost A1 - Rode, Anja A1 - Krueger, Sylvia A1 - Lee, Dongtao N. A1 - Wolf, C. Roland A1 - Scheer, Nico T1 - Quantitative prediction of human pregnane X receptor and cytochrome P450 3A4 mediated drug-drug interaction in a novel multiple humanized mouse line JF - Molecular Pharmacology Y1 - 2011 U6 - http://dx.doi.org/10.1124/mol.111.071845 SN - 1521-0111 VL - 80 IS - 33 SP - 518 EP - 528 PB - ASPET CY - Bethesda, Md. ER - TY - JOUR A1 - Jordan, Sabine D. A1 - Krüger, Markus A1 - Willmes, Diana M. A1 - Redemann, Nora A1 - Wunderlich, F. Thomas A1 - Brönneke, Hella S. A1 - Merkwirth, Carsten A1 - Kashkar, Hamid A1 - Olkkonen, Vesa M. A1 - Böttger, Thomas A1 - Braun, Thomas A1 - Seibler, Jost A1 - Brüning, Jens C. T1 - Obesity-induced overexpression of miRNA-143 inhibits insulin-stimulated AKT activation and impairs glucose metabolism JF - Nature Cell Biology N2 - The contribution of altered post-transcriptional gene silencing to the development of insulin resistance and type 2 diabetes mellitus so far remains elusive. Here, we demonstrate that expression of microRNA (miR)-143 and 145 is upregulated in the liver of genetic and dietary mouse models of obesity. Induced transgenic overexpression of miR-143, but not miR-145, impairs insulin-stimulated AKT activation and glucose homeostasis. Conversely, mice deficient for the miR-143–145 cluster are protected from the development of obesity-associated insulin resistance. Quantitative-mass-spectrometry-based analysis of hepatic protein expression in miR-143-overexpressing mice revealed miR-143-dependent downregulation of oxysterol-binding-protein-related protein (ORP) 8. Reduced ORP8 expression in cultured liver cells impairs the ability of insulin to induce AKT activation, revealing an ORP8-dependent mechanism of AKT regulation. Our experiments provide direct evidence that dysregulated post-transcriptional gene silencing contributes to the development of obesity-induced insulin resistance, and characterize the miR-143–ORP8 pathway as a potential target for the treatment of obesity-associated diabetes. Y1 - 2011 U6 - http://dx.doi.org/10.1038/ncb2211 SN - 1465-7392 VL - 13 IS - 4 SP - 434 EP - 446 PB - Nature CY - New York ER - TY - CHAP A1 - Seibler, Jost A1 - Schwenk, Frieder T1 - Transgenic RNAi Applications in the Mouse T2 - Methods in Enzymology : Guide to Techniques in Mouse Development, Part B: Mouse Molecular Genetics. 2nd Edition Y1 - 2010 SN - 978-0-12-384880-2 N1 - Methods in Enzymology : Vol. 477 SP - 367 EP - 386 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Glaser, Stefan A1 - Lubitz, Sandra A1 - Loveland, Kate L. A1 - Ohbo, Kazu A1 - Robb, Lorraine A1 - Schwenk, Frieder A1 - Seibler, Jost A1 - Roellig, Daniela A1 - Kranz, Andrea A1 - Anastassiadis, Konstantinos A1 - Stewart, A. Francis T1 - The histone 3 lysine 4 methyltransferase, Mll2, is only required briefly in development and spermatogenesis JF - Epigenetics & Chromatin Y1 - 2009 U6 - http://dx.doi.org/10.1186/1756-8935-2-5 SN - 1756-8935 VL - 2 IS - 5 SP - 1 EP - 16 ER - TY - JOUR A1 - Kotnik, Katarina A1 - Popova, Elena A1 - Todiras, Mihail A1 - Mori, Marcelo A. A1 - Alenina, Natalia A1 - Seibler, Jost A1 - Bader, Michael T1 - Inducible transgenic rat model for diabetes mellitus based on shRNA-mediated gene knockdown JF - Plos One Y1 - 2009 U6 - http://dx.doi.org/10.1371/journal.pone.0005124 VL - 4 IS - 4 SP - e5124 PB - Plos CY - San Francisco, California, US ER - TY - JOUR A1 - Herold, Marco J. A1 - Brandt, Jens van den A1 - Seibler, Jost A1 - Reichardt, Holger M. T1 - Inducible and reversible gene silencing by stable integration of an shRNA-encoding lentivirus in transgenic rats JF - Proceedings of the National Academy of Sciences of the United States of America Y1 - 2008 U6 - http://dx.doi.org/10.1073/pnas.0806213105 SN - 1091-6490 VL - 105 IS - 47 SP - 18507 EP - 18512 ER - TY - JOUR A1 - Koch, Linda A1 - Wunderlich, F. Thomas A1 - Seibler, Jost A1 - Könner, A. Christine A1 - Hampel, Brigitte A1 - Irlenbusch, Sigrid A1 - Brabant, Georg A1 - Kahn, C. Ronald A1 - Schwenk, Frieder A1 - Brüning, Jens C. T1 - Central insulin action regulates peripheral glucose and fat metabolism in mice JF - The Journal of Clinical Investigation (JCI) Y1 - 2008 U6 - http://dx.doi.org/10.1172/JCI31073 SN - 1558-8238 VL - 118 IS - 6 SP - 2132 EP - 2147 ER - TY - JOUR A1 - Christoph, Thomas A1 - Bahrenberg, Gregor A1 - Vry, Jean de A1 - Englberger, Werner A1 - Erdmann, Volker A. A1 - Frech, Moritz A1 - Kögel, Babette A1 - Röhl, Thomas A1 - Schiene, Klaus A1 - Schröder, Wolfgang A1 - Seibler, Jost A1 - Kurreck, Jens T1 - Investigation of TRPV1 loss-of-function phenotypes in transgenic shRNA expressing and knockout mice JF - Molecular and Cellular Neuroscience Y1 - 2008 U6 - http://dx.doi.org/10.1016/j.mcn.2007.12.006 SN - 1044-7431 VL - 37 IS - 3 SP - 579 EP - 589 ER - TY - JOUR A1 - Seibler, Jost A1 - Kleinridders, Andre A1 - Küter-Luks, Birgit A1 - Niehaves, Sandra A1 - Brüning, Jens C. A1 - Schwenk, Frieder T1 - Reversible gene knockdown in mice using a tight, inducible shRNA expression system JF - Nucleic Acids Research Y1 - 2007 U6 - http://dx.doi.org/10.1093/nar/gkm122 SN - 1362-4962 VL - 35 IS - 7 SP - e54 ER - TY - JOUR A1 - Scheer, Nico A1 - Snaith, Mike A1 - Wolf, C. Roland A1 - Seibler, Jost T1 - Generation and utility of genetically humanized mouse models JF - Drug Discovery Today Y1 - 2013 U6 - http://dx.doi.org/10.1016/j.drudis.2013.07.007 SN - 1359-6446 VL - Vol 18 IS - 23-24 SP - 1200 EP - 1211 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Bouwman, Peter A1 - Gulden, Hanneke van der A1 - Heijden, Ingrid van der A1 - Drost, Rinske A1 - Klijn, Christiaan N. A1 - Prasetyanti, Pramudita A1 - Pieterse, Mark A1 - Wientjens, Ellen A1 - Seibler, Jost A1 - Hogervorst, Frank B. L. A1 - Jonkers, Jos T1 - A High-Throughput Functional Complementation Assay for Classification of BRCA1 Missense Variants JF - Cancer Discovery Y1 - 2013 U6 - http://dx.doi.org/10.1158/2159-8290.CD-13-0094 SN - 2159-8290 IS - 3 SP - 1142 EP - 1152 ER - TY - JOUR A1 - Michalak, Ewa Malgorzata A1 - Nacerddine, Karim A1 - Pietersen, Alexandra A1 - Beuger, Vincent A1 - Pawlitzky, Inka A1 - Cornelissen-Steijger, Paulien A1 - Wientjens, Ellen A1 - Tanger, Ellen A1 - Seibler, Jost A1 - Lohuizen, Maarten van A1 - Jonkers, Jos T1 - Polycomb group gene Ezh2 regulates mammary gland morphogenesis and maintains the luminal progenitor pool JF - Stem Cells Y1 - 2013 U6 - http://dx.doi.org/10.1002/stem.1437 SN - 1549-4918 VL - Vol 31 IS - 9 SP - 1910 EP - 1920 PB - Oxford University Press CY - Oxford ER - TY - JOUR A1 - Gebeshuber, Christoph A. A1 - Kornauth, Christoph A1 - Dong, Lihua A1 - Sierig, Ralph A1 - Seibler, Jost A1 - Reiss, Martina A1 - Tauber, Stefanie A1 - Bilban, Martin A1 - Wang, Shijun A1 - Kain, Renate A1 - Böhmig, Georg A. A1 - Moeller, Marcus J. A1 - Gröne, Hermann-Josef A1 - Englert, Christoph A1 - Martinez, Javier A1 - Kerjaschki, Dontscho T1 - Focal segmental glomerulosclerosis is induced by microRNA-193a and its downregulation of WT1 JF - Nature Medicine Y1 - 2013 U6 - http://dx.doi.org/10.1038/nm.3142 SN - 1078-8956 VL - 19 IS - 4 SP - 481 EP - 487 ER - TY - JOUR A1 - Kornfeld, Jan-Wilhelm A1 - Baitzel, Catherina A1 - Könner, A. Christine A1 - Nicholls, Hayley T. A1 - Vogt, Merly C. A1 - Herrmanns, Karolin A1 - Scheja, Ludger A1 - Haumaitre, Cécile A1 - Wolf, Anna M. A1 - Knippschild, Uwe A1 - Seibler, Jost A1 - Cereghini, Silvia A1 - Heeren, Joerg A1 - Stoffel, Markus A1 - Brüning, Jens C. T1 - Obesity-induced overexpression of miR-802 impairs glucose metabolism through silencing of Hnf1b JF - Nature Y1 - 2013 SN - 0028-0836 U6 - http://dx.doi.org/10.1038/nature11793 VL - 494 IS - 7435 SP - 111 EP - 115 PB - Springer Nature CY - Cham ER - TY - JOUR A1 - Inagaki, Akiko A1 - Sleddens-Linkels, Esther A1 - Wassenaar, Evelyne A1 - Ooms, Marja A1 - Cappellen, Wiggert A. van A1 - Hoeijmakers, Jan H. J. A1 - Seibler, Jost A1 - Vogt, Thomas F. A1 - Shin, Myung K. A1 - Grootegoed, J. Anton A1 - Baarends, Willy M. T1 - Meiotic functions of RAD18 JF - Journal of Cell Science Y1 - 2011 U6 - http://dx.doi.org/10.1242/jcs.081968 SN - 1477-9137 VL - 124 IS - 16 SP - 2837 EP - 2850 PB - Company of Biologists Limited CY - Cambridge ER - TY - JOUR A1 - Schiffels, Johannes A1 - Selmer, Thorsten T1 - A flexible toolbox to study protein-assisted metalloenzyme assembly in vitro JF - Biotechnology and Bioengineering Y1 - 2015 U6 - http://dx.doi.org/10.1002/bit.25658 SN - 1097-0290 VL - 112 IS - 11 SP - 2360 EP - 2372 PB - Wiley CY - Weinheim ER - TY - JOUR A1 - Voigt, Birgit A1 - Albrecht, Dirk A1 - Sievers, Susanne A1 - Becher, Dörte A1 - Bongaerts, Johannes A1 - Evers, Stefan A1 - Schweder, Thomas A1 - Maurer, Karl-Heinz A1 - Hecker, Michael T1 - High-resolution proteome maps of Bacillus licheniformis cells growing in minimal medium JF - Proteomics Y1 - 2015 U6 - http://dx.doi.org/10.1002/pmic.201400504 SN - 1615-9861 VL - 15 IS - 15 SP - 2629 EP - 2633 PB - Wiley CY - Weinheim ER - TY - JOUR A1 - Molinnus, Denise A1 - Bäcker, Matthias A1 - Siegert, Petra A1 - Willenberg, H. A1 - Poghossian, Arshak A1 - Keusgen, M. A1 - Schöning, Michael Josef T1 - Detection of Adrenaline Based on Substrate Recycling Amplification JF - Procedia Engineering N2 - An amperometric enzyme biosensor has been applied for the detection of adrenaline. The adrenaline biosensor has been prepared by modification of an oxygen electrode with the enzyme laccase that operates at a broad pH range between pH 3.5 to pH 8. The enzyme molecules were immobilized via cross-linking with glutaraldehyde. The sensitivity of the developed adrenaline biosensor in different pH buffer solutions has been studied. Y1 - 2015 U6 - http://dx.doi.org/10.1016/j.proeng.2015.08.708 SN - 1877-7058 N1 - Eurosensors 2015 VL - 120 SP - 540 EP - 543 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Pilas, Johanna A1 - Mariano, K. A1 - Keusgen, M. A1 - Selmer, Thorsten A1 - Schöning, Michael Josef T1 - Optimization of an Enzyme-based Multi-parameter Biosensor for Monitoring Biogas Processes JF - Procedia Engineering Y1 - 2015 U6 - http://dx.doi.org/10.1016/j.proeng.2015.08.702 SN - 1877-7058 N1 - Part of special issue "Eurosensors 2015" VL - 120 SP - 532 EP - 535 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Plum, Leona A1 - Ma, Xiaosong A1 - Hampel, Brigitte A1 - Balthasar, Nina A1 - Coppari, Roberto A1 - Münzberg, Heike A1 - Shanabrough, Marya A1 - Burdakov, Denis A1 - Rother, Eva A1 - Janoschek, Ruth A1 - Alber, Jens A1 - Belgardt, Bengt F. A1 - Koch, Linda A1 - Seibler, Jost A1 - Schenk, Frieder A1 - Fekete, Csaba A1 - Suzuki, Akira A1 - Mak, Tak W. A1 - Krone, Wilhelm A1 - Horvath, Tamas L. A1 - Ashcroft, Frances M. A1 - Brüning, Jens C. T1 - Enhanced PIP3 signaling in POMC neurons causes KATP channel activation and leads to diet-sensitive obesity JF - The Journal of Clinical Investigation (JCI) Y1 - 2006 U6 - http://dx.doi.org/10.1172/JCI27123 SN - 1558-8238 VL - 116 IS - 7 SP - 1886 EP - 1901 ER - TY - JOUR A1 - Seibler, Jost A1 - Küter-Luks, Birgit A1 - Kern, Heidrun A1 - Streu, Sandra A1 - Plum, Leona A1 - Maurer, Jan A1 - Kühn, Ralf A1 - Brüning, Jens C. A1 - Schwenk, Frieder T1 - Single copy shRNA configuration for ubiquitous gene knockdown in mice JF - Nucleic Acids Research Y1 - 2005 U6 - http://dx.doi.org/10.1093/nar/gni065 SN - 1362-4962 VL - 33 IS - 7 SP - e67 ER - TY - JOUR A1 - Seibler, Jost A1 - Zevnik, Branko A1 - Küter-Luks, Birgit A1 - Andreas, Susanne A1 - Kern, Heidrun A1 - Hennek, Thomas A1 - Rode, Anja A1 - Heimann, Cornelia A1 - Faust, Nicole A1 - Kauselmann, Gunther A1 - Schoor, Michael A1 - Jaenisch, Rudolf A1 - Rajewsky, Klaus A1 - Kühn, Ralf A1 - Schwenk, Frieder T1 - Rapid generation of inducible mouse mutants JF - Nucleic Acids Research Y1 - 2003 U6 - http://dx.doi.org/10.1093/nar/gng012 SN - 1362-4962 VL - 33 IS - 4 SP - e12 ER - TY - JOUR A1 - Goetze, Sandra A1 - Baer, Alexandra A1 - Winkelmann, Silke A1 - Nehlsen, Kristina A1 - Seibler, Jost A1 - Maass, Karin A1 - Bode, Jürgen T1 - Performance of genomic bordering elements at predefined genomic loci JF - Molecular and Cellular Biology Y1 - 2005 U6 - http://dx.doi.org/10.1128/MCB.25.6.2260-2272.2005 SN - 1098-5549 VL - 25 IS - 6 SP - 2260 EP - 2272 ER - TY - JOUR A1 - Cesari, Francesca A1 - Rennekampff, Verena A1 - Vintersten, Kristina A1 - Vuong, Lam Giang A1 - Seibler, Jost A1 - Bode, Jürgen A1 - Wiebel, Franziska F. A1 - Nordheim, Alfred T1 - Elk-1 knock-out mice engineered by Flp recombinase-mediated cassette exchange JF - Genesis : The Journal of Genetics and Development Y1 - 2004 U6 - http://dx.doi.org/10.1002/gene.20003 SN - 1526-968X VL - 38 IS - 2 SP - 87 EP - 92 ER - TY - JOUR A1 - Takenaga, Shoko A1 - Schneider, Benno A1 - Erbay, E. A1 - Biselli, Manfred A1 - Schnitzler, Thomas A1 - Schöning, Michael Josef A1 - Wagner, Torsten T1 - Fabrication of biocompatible lab-on-chip devices for biomedical applications by means of a 3D-printing process JF - Physica status solidi (a) N2 - A new microfluidic assembly method for semiconductor-based biosensors using 3D-printing technologies was proposed for a rapid and cost-efficient design of new sensor systems. The microfluidic unit is designed and printed by a 3D-printer in just a few hours and assembled on a light-addressable potentiometric sensor (LAPS) chip using a photo resin. The cell growth curves obtained from culturing cells within microfluidics-based LAPS systems were compared with cell growth curves in cell culture flasks to examine biocompatibility of the 3D-printed chips. Furthermore, an optimal cell culturing within microfluidics-based LAPS chips was achieved by adjusting the fetal calf serum concentrations of the cell culture medium, an important factor for the cell proliferation. Y1 - 2015 U6 - http://dx.doi.org/10.1002/pssa.201532053 SN - 1862-6319 VL - 212 IS - 6 SP - 1347 EP - 1352 PB - Wiley CY - Weinheim ER - TY - JOUR A1 - Bode, Jürgen A1 - Schlake, Thomas A1 - Iber, Michaela A1 - Schübeler, Dirk A1 - Seibler, Jost A1 - Snezhkov, Evgeney A1 - Nikolaev, Lev T1 - The transgeneticist's toolbox: novel methods for the targeted modification of eukaryotic genomes JF - Biological Chemistry Y1 - 2000 U6 - http://dx.doi.org/10.1515/BC.2000.103 SN - 1431-6730 VL - 381 IS - 9-10 SP - 801 EP - 813 ER - TY - JOUR A1 - Feng, Yong-Qing A1 - Seibler, Jost A1 - Alami, Raouf A1 - Eisen, Andrew A1 - Westerman, Karen A. A1 - Leboulch, Philippe A1 - Fiering, Steven A1 - Bouhassira, Eric E. T1 - Site-specific chromosomal integration in mammalian cells: highly efficient CRE recombinase-mediated cassette exchange JF - Journal of Molecular Biology Y1 - 1999 U6 - http://dx.doi.org/10.1006/jmbi.1999.3113 SN - 0022-2836 VL - 292 IS - 4 SP - 779 EP - 785 ER - TY - JOUR A1 - Seibler, Jost A1 - Schübeler, Dirk A1 - Fiering, Steven A1 - Groudine, Mark A1 - Bode, Jürgen T1 - DNA cassette exchange in ES cells mediated by Flp recombinase: an efficient strategy for repeated modification of tagged loci by marker-free constructs JF - Biochemistry Y1 - 1998 U6 - http://dx.doi.org/10.1021/bi980288t SN - 1520-4995 VL - 37 IS - 18 SP - 6229 EP - 6234 ER - TY - JOUR A1 - Seibler, Jost A1 - Bode, Jürgen T1 - Double-reciprocal crossover mediated by FLP-recombinase: a concept and an assay JF - Biochemistry Y1 - 1997 SN - 1520-4995 VL - 36 IS - 7 SP - 1740 EP - 1747 ER - TY - JOUR A1 - Bode, J. A1 - Bartsch, J. A1 - Boulikas, T. A1 - Iber, M. A1 - Mielke, C. A1 - Schübeler, D. A1 - Seibler, Jost A1 - Benham, C. T1 - Transcription-promoting genomic sites in mammalia: their elucidation and architectural principles JF - Gene therapy & molecular biology Y1 - 1998 SN - 1529-9120 VL - 1 IS - 1 SP - 1 EP - 29 ER - TY - JOUR A1 - Iber, Michaela A1 - Schübeler, Dirk A1 - Seibler, Jost A1 - Höxter, Maria A1 - Bode, Jürgen T1 - Efficient FACS selection procedure for cells undergoing Flp-mediated site-specific conversions JF - Technical Tips Online Y1 - 1998 U6 - http://dx.doi.org/10.1016/S1366-2120(08)70132-6 VL - 4 IS - 1 SP - 25 EP - 29 ER - TY - JOUR A1 - Wiegand, Sandra A1 - Voigt, Birgit A1 - Albrecht, Dirk A1 - Bongaerts, Johannes A1 - Evers, Stefan A1 - Hecker, Michael A1 - Daniel, Rolf A1 - Liesegang, Heiko T1 - Fermentation stage-dependent adaptations of Bacillus licheniformis during enzyme production JF - Microbial Cell Factories Y1 - 2013 U6 - http://dx.doi.org/10.1186/1475-2859-12-120 SN - 1475-2859 VL - 12 SP - 120 PB - Biomed Central CY - London ER - TY - JOUR A1 - Henken, F. E. A1 - Oosterhuis, K. A1 - Öhlschläger, Peter A1 - Bosch, L. A1 - Hooijberg, E. A1 - Haanen, J. B. A. G. A1 - Steenbergen, R. D. M. T1 - Preclinical safety evaluation of DNA vaccines encoding modified HPV16 E6 and E7 JF - Vaccine N2 - Persistent infection with high-risk human papillomaviruses (hrHPV) can result in the formation of anogenital cancers. As hrHPV proteins E6 and E7 are required for cancer initiation and maintenance, they are ideal targets for immunotherapeutic interventions. Previously, we have described the development of DNA vaccines for the induction of HPV16 E6 and E7 specific T cell immunity. These vaccines consist of ‘gene-shuffled’ (SH) versions of HPV16 E6 and E7 that were fused to Tetanus Toxin Fragment C domain 1 (TTFC) and were named TTFC-E6SH and TTFC-E7SH. Gene-shuffling was performed to avoid the risk of inducing malignant transformation at the vaccination site. Here, we describe the preclinical safety evaluation of these candidate vaccines by analysis of their transforming capacity in vitro using established murine fibroblasts (NIH 3T3 cells) and primary human foreskin keratinocytes (HFKs). We demonstrate that neither ectopic expression of TTFC-E6SH and TTFC-E7SH alone or in combination enabled NIH 3T3 cells to form colonies in soft agar. In contrast, expression of HPV16 E6WT and E7WT alone or in combination resulted in effective transformation. Similarly, retroviral transduction of HFKs from three independent donors with both TTFC-E6SH and TTFC-E7SH alone or in combination did not show any signs of immortalization. In contrast, the combined expression of E6WT and E7WT induced immortalization in HFKs from all donors. Based on these results we consider it justified to proceed to clinical evaluation of DNA vaccines encoding TTFC-E6SH and TTFC-E7SH in patients with HPV16 associated (pre)malignancies. Y1 - 2012 U6 - http://dx.doi.org/10.1016/j.vaccine.2012.04.013 SN - 0264-410X VL - 30 IS - 28 SP - 4259 EP - 4266 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Immel, Timo A1 - Grützke, Martin A1 - Späte, Anne-Katrin A1 - Groth, Ulrich A1 - Öhlschläger, Peter A1 - Huhn, Thomas T1 - Synthesis and X-ray structure analysis of a heptacoordinate titanium(IV)-bis-chelate with enhanced in vivo antitumor efficacy JF - Chemical Communications N2 - Chelate stabilization of a titanium(IV)–salan alkoxide by ligand exchange with 2,6-pyridinedicarboxylic acid (dipic) resulted in heptacoordinate complex 3 which is not redox-active, stable on silica gel and has increased aqueous stability. 3 is highly toxic in HeLa S3 and Hep G2 and has enhanced antitumor efficacy in a mouse cervical-cancer model. Y1 - 2012 U6 - http://dx.doi.org/10.1039/C2CC31624B SN - 1364-548X VL - 48 IS - 46 SP - 5790 EP - 5792 PB - Royal Society of Chemistry CY - Cambridge ER - TY - CHAP A1 - Srivastava, Alok A1 - Knolle, F. A1 - Schnug, E. A1 - Scherer, Ulrich W. T1 - Study of Trace Elements in Water Bodies of the Harz Mts. Region, Germany using Total Reflection X Ray Fluorescence (TXRF) T2 - DAE-BRNS 12th National Symposium on Nuclear and Radiochemistry NUCAR 2015, Feb. 9-13, Mumbai, India, Mumbai, India; 02/2015 Y1 - 2015 SP - 355 EP - 356 ER - TY - CHAP A1 - Srivastava, Alok A1 - Knolle, Friedhart A1 - Hoyler, Friedrich A1 - Scherer, Ulrich W. A1 - Schnug, Ewald T1 - Uranium Toxicity in the State of Punjab in North-Western India T2 - Management of Natural Resources in a Changing Environment N2 - Lately there has been an increasing concern about uranium toxicity in some districts of Punjab State located in the North Western part of India after the publication of a report (Blaurock-Busch et al. 2010) which showed that the concentration of uranium in hair and urine of children suffering from physical deformities, neurological and mental disorder from Malwa region (Fig. 1) of Punjab State was manifold higher than the reference ranges. A train which connects the affected region with the nearby city of Bikaner which has a Cancer Hospital has been nicknamed as Cancer Express due to the frenzy generated on account of uranium related toxicity. Y1 - 2015 SN - 978-3-319-12559-6 U6 - http://dx.doi.org/10.1007/978-3-319-12559-6_21 SP - 271 EP - 275 PB - Springer CY - Cham ER - TY - JOUR A1 - Cehreli, Ruksan A1 - Akpinar, Hale A1 - Temiz Artmann, Aysegül A1 - Sagol, Ozgul T1 - Effects of Glutamine and Omega-3 Fatty Acids on Erythrocyte Deformability and Oxidative Damage in Rat Model of Enterocolitis JF - Gastroenterology Research Y1 - 2015 U6 - http://dx.doi.org/10.14740/gr683w SN - 1918-2813 VL - 8 IS - 5 SP - 265 EP - 273 ER - TY - PAT A1 - O'Connell, Timothy A1 - Siegert, Petra A1 - Maurer, Karl-Heinz A1 - Schiedel, Marc-Steffen A1 - Vockenroth, Inga Kerstin T1 - Method for improving the cleaning action of a detergent or cleaning agent [Internationale Patentanmeldung] T1 - Verfahren zur Verbesserung der Reinigungsleistung eines Wasch- oder Reinigungsmittels Y1 - 2010 SP - 1 EP - 15 PB - WIPO CY - Genf ER - TY - CHAP A1 - Breuer, Lars A1 - Raue, Markus A1 - Mang, Thomas A1 - Schöning, Michael Josef A1 - Thoelen, Ronald A1 - Wagner, Torsten T1 - Light-stimulated hydrogel actuators with incorporated graphene oxide for microfluidic applications T2 - 12. Dresdner Sensor-Symposium 2015 Y1 - 2015 U6 - http://dx.doi.org/10.5162/12dss2015/P5.8 SP - 206 EP - 209 ER - TY - JOUR A1 - Breuer, Lars A1 - Raue, Markus A1 - Strobel, M. A1 - Mang, Thomas A1 - Schöning, Michael Josef A1 - Thoelen, R. A1 - Wagner, Torsten T1 - Hydrogels with incorporated graphene oxide as light-addressable actuator materials for cell culture environments in lab-on-chip systems JF - Physica status solidi (a) N2 - Abstractauthoren Graphene oxide (GO) nanoparticles were incorporated in temperature-sensitive Poly(N-isopropylacrylamide) (PNIPAAm) hydrogels. The nanoparticles increase the light absorption and convert light energy into heat efficiently. Thus, the hydrogels with GO can be stimulated spatially resolved by illumination as it was demonstrated by IR thermography. The temporal progression of the temperature maximum was detected for different concentrations of GO within the polymer network. Furthermore, the compatibility of PNIPAAm hydrogels with GO and cell cultures was investigated. For this purpose, culture medium was incubated with hydrogels containing GO and the viability and morphology of chinese hamster ovary (CHO) cells was examined after several days of culturing in presence of this medium. Y1 - 2016 U6 - http://dx.doi.org/10.1002/pssa.201533056 SN - 1862-6300 VL - 213 IS - 6 SP - 1520 EP - 1525 PB - Wiley-VCH CY - Weinheim ER - TY - CHAP A1 - Kasper, Katharina A1 - Schiffels, Johannes A1 - Krafft, Simone A1 - Kuperjans, Isabel A1 - Elbers, Gereon A1 - Selmer, Thorsten T1 - Biogas Production on Demand Regulated by Butyric Acid Addition T2 - IOP Conference Series: Earth and Environmental Science. Bd. 32 Y1 - 2016 U6 - http://dx.doi.org/10.1088/1755-1315/32/1/012009 SN - 1755-1315 N1 - ICARET 2016, International Conference on Advances in Renewable Energy and Technologies, Putrajaya, MY, Feb 23-25, 2016 VL - 32 SP - 012009/1 EP - 012009/4 ER - TY - BOOK A1 - Müller, Bodo A1 - Rath, Walter T1 - Formulierung von Kleb- und Dichtstoffen: das kompetente Lehrbuch für Studium und Praxis Y1 - 2015 SN - 978-3-86630-605-9 PB - Vincentz Network CY - Hannover ET - 3., vollst. überarb. Aufl. ER - TY - JOUR A1 - Niedermeyer, Angela A1 - Zhou, Bei A1 - Dursun, Gözde A1 - Temiz Artmann, Aysegül A1 - Markert, Bernd T1 - An examination of tissue engineered scaffolds in a bioreactor JF - Proceedings in Applied Mathematics and Mechanics PAMM N2 - Replacement tissues, designed to fill in articular cartilage defects, should exhibit the same properties as the native material. The aim of this study is to foster the understanding of, firstly, the mechanical behavior of the material itself and, secondly, the influence of cultivation parameters on cell seeded implants as well as on cell migration into acellular implants. In this study, acellular cartilage replacement material is theoretically, numerically and experimentally investigated regarding its viscoelastic properties, where a phenomenological model for practical applications is developed. Furthermore, remodeling and cell migration are investigated. Y1 - 2016 U6 - http://dx.doi.org/10.1002/pamm.201610038 SN - 1617-7061 N1 - Joint Annual Meeting of DMV and GAMM 2016, 87th Annual Meeting of the International Association of Applied Mathematics and Mechanics (GAMM) and Deutsche Mathematiker-Vereinigung (DMV), Braunschweig, DE, Mar 7-11, 2016 VL - 16 IS - 1 SP - 99 EP - 100 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Pinkenburg, Olaf A1 - Schiffels, Johannes A1 - Selmer, Thorsten T1 - Das CoLibry-Konzept – ein Werkzeugkasten für die Synthetische Biologie: Bioproduktion JF - BIOspektrum N2 - Regardless of size or destination, synthetic biology starts with com-parably small information units, which need to be combined and properly arranged in order to achieve a certain goal. This may be the de novo synthesis of individual genes from oligonucleotides, a shuffling of protein domains in order to create novel biocatalysts, the assembly of multiple enzyme encoding genes in metabolic pathway design, or strain development at the production stage. The CoLibry concept has been designed in order to close the gap between recombinant production of individual genes and genome editing. Y1 - 2016 U6 - http://dx.doi.org/10.1007/s12268-016-0734-8 VL - 22 IS - 6 SP - 593 EP - 595 PB - Springer CY - Berlin ER - TY - JOUR A1 - Heinze, D. A1 - Mang, Thomas A1 - Popescu, C. A1 - Weichold, O. T1 - Effect of side chain length and degree of polymerization on the decomposition and crystallization behaviour of chlorinated poly(vinyl ester) oligomers JF - Thermochimica Acta N2 - Four members of a homologous series of chlorinated poly(vinyl ester) oligomers CCl₃–(CH₂CH (OCO(CH₂)ₘCH₃))ₙ–Cl with degrees of polymerization of 10 and 20 were prepared by telomerisation using carbon tetrachloride. The number of side chain carbon atoms ranges from 2 (poly(vinyl acetate) to 18 (poly(vinyl stearate)). The effect of the n-alkyl side chain length and of the degree of polymerization on the thermal stability and crystallization behaviour of the synthesized compounds was investigated. All oligomers degrade in two major steps by first losing HCl and side chains with subsequent breakdown of the backbone. The members with short side chains, up to poly(vinyl octanoate), are amorphous and show internal plasticization, whereas those with high number of side chain carbon atoms are semi-crystalline due to side-chain crystallization. A better packing for poly(vinyl stearate) is also noticeable. The glass transition and melting temperatures as well as the onset temperature of decomposition are influenced to a larger extent by the side chain length than by the degree of polymerization. Thermal stability is improved if both the size and number of side chains increase, but only a long side chain causes a significant increase of the resistance to degradation. This results in a stabilization of PVAc so that oligomers from poly(vinyl octanoate) on are stable under atmospheric conditions. Thus, the way to design stable, chlorinated PVEs oligomers is to use a long n-alkyl side chain. Y1 - 2016 U6 - http://dx.doi.org/10.1016/j.tca.2016.05.015 SN - 0040-6031 (electronic) VL - 637 SP - 143 EP - 153 PB - Elsevier CY - Amsterdam ER - TY - BOOK A1 - Feuerriegel, Uwe T1 - Verfahrenstechnik mit EXCEL: Verfahrenstechnische Berechnungen effektiv durchführen und professionell dokumentieren Y1 - 2016 SN - 978-3-658-02902-9 U6 - http://dx.doi.org/10.1007/978-3-658-02903-6 N1 - 152 Abbildungen, 94 Abbildungen in Farbe Gedruckt in der Bibliothek unter der Signatur: 21 ZNK 11 PB - Springer Fachmedien CY - Wiesbaden ER - TY - BOOK A1 - Lauth, Jakob T1 - Physikalische Chemie, 5: Elektrochemie Y1 - 2016 SN - 978-3-662-47559-1 PB - Springer CY - Berlin ER - TY - BOOK A1 - Lauth, Jakob T1 - Physikalische Chemie, 4: Reaktionskinetik Y1 - 2016 SN - 978-3-662-47674-1 PB - Springer CY - Berlin ER - TY - BOOK A1 - Lauth, Jakob T1 - Physikalische Chemie, 3: Phasengleichgewichte Y1 - 2016 SN - 978-3-662-47571-3 PB - Springer CY - Berlin ER - TY - BOOK A1 - Lauth, Jakob T1 - Physikalische Chemie, 2: Chemische Thermodynamik Y1 - 2016 SN - 978-3-662-47621-5 PB - Springer CY - Berlin ER - TY - BOOK A1 - Lauth, Jakob T1 - Physikalische Chemie, 1: Grundlagen der Thermodynamik und Verhalten der Gase Y1 - 2016 SN - 978-3-662-47676-5 PB - Springer CY - Berlin ER - TY - BOOK A1 - Lauth, Jakob A1 - Kowalczyk, Jürgen T1 - Einführung in die Physik und Chemie der Grenzflächen und Kolloide Y1 - 2016 SN - 978-3-662-47018-3 U6 - http://dx.doi.org/10.1007/978-3-662-47018-3 PB - Springer CY - Berlin ER - TY - JOUR A1 - Molinnus, Denise A1 - Sorich, Maren A1 - Bartz, Alexander A1 - Siegert, Petra A1 - Willenberg, Holger S. A1 - Lisdat, Fred A1 - Poghossian, Arshak A1 - Keusgen, Michael A1 - Schöning, Michael Josef T1 - Towards an adrenaline biosensor based on substrate recycling amplification in combination with an enzyme logic gate JF - Sensors and Actuators B: Chemical N2 - An amperometric biosensor using a substrate recycling principle was realized for the detection of low adrenaline concentrations (1 nM) by measurements in phosphate buffer and Ringer’s solution at pH 6.5 and pH 7.4, respectively. In proof-of-concept experiments, a Boolean logic-gate principle has been applied to develop a digital adrenaline biosensor based on an enzyme AND logic gate. The obtained results demonstrate that the developed digital biosensor is capable for a rapid qualitative determination of the presence/absence of adrenaline in a YES/NO statement. Such digital biosensor could be used in clinical diagnostics for the control of a correct insertion of a catheter in the adrenal veins during adrenal venous-sampling procedure. Y1 - 2016 U6 - http://dx.doi.org/10.1016/j.snb.2016.06.064 SN - 0925-4005 VL - 237 SP - 190 EP - 195 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Ghosch, S. A1 - Baier, M. A1 - Schütz, J. A1 - Schneider, Felix A1 - Scherer, Ulrich W. T1 - Analysis of electronic autoradiographs by mathematical post-processing JF - Radiation Effects and Defects in Solids: Incorporating plasma science and plasma technology N2 - Autoradiography is a well-established method of nuclear imaging. When different radionuclides are present simultaneously, additional processing is needed to distinguish distributions of radionuclides. In this work, a method is presented where aluminium absorbers of different thickness are used to produce images with different cut-off energies. By subtracting images pixel-by-pixel one can generate images representing certain ranges of β-particle energies. The method is applied to the measurement of irradiated reactor graphite samples containing several radionuclides to determine the spatial distribution of these radionuclides within pre-defined energy windows. The process was repeated under fixed parameters after thermal treatment of the samples. The greyscale images of the distribution after treatment were subtracted from the corresponding pre-treatment images. Significant changes in the intensity and distribution of radionuclides could be observed in some samples. Due to the thermal treatment parameters the most significant differences were observed in the ³H and ¹⁴C inventory and distribution. Y1 - 2016 U6 - http://dx.doi.org/10.1080/10420150.2016.1155587 SN - 1029-4953 VL - 171 IS - 1-2 SP - 161 EP - 172 PB - Taylor & Francis CY - London ER - TY - BOOK A1 - Lauth, Jakob A1 - Kowalczyk, Jürgen T1 - Thermodynamik : eine Einführung Y1 - 2015 SN - 978-3-662-46228-7 U6 - http://dx.doi.org/10.1007/978-3-662-46229-4 PB - Springer Spektrum CY - Berlin ER - TY - JOUR A1 - Pasteur, Aline A1 - Tippkötter, Nils A1 - Kampeis, Percy A1 - Ulber, Roland T1 - Optimization of high gradient magnetic separation filter units for the purification of fermentation products JF - IEEE TRANSACTIONS ON MAGNETICS N2 - High gradient magnetic separation (HGMS) has been established since the early 1970s. A more recent application of these systems is the use in bioprocesses. To integrate the HGMS in a fermentation process, it is necessary to optimize the separation matrix with regard to the magnetic separation characteristics and permeability of the non-magnetizable components of the fermentation broth. As part of the work presented here, a combined fluidic and magnetic force finite element model simulation was created using the software COMSOL Multiphysics and compared with separation experiments. Finally, as optimal lattice orientation of the separation matrix, a transversal rhombohedral arrangement was defined. The high suitability of the new filter matrix has been verified by separation experiments. Y1 - 2014 U6 - http://dx.doi.org/10.1109/TMAG.2014.2325535 SN - 0018-9464 N1 - Article Sequence Number: 5000607 INSPEC Accession Number: 14663042 VL - 50 IS - 10 SP - Artikel 5000607 PB - IEEE CY - New York, NY ER - TY - JOUR A1 - Tippkötter, Nils A1 - Wollny, Steffen A1 - Suck, Kirstin A1 - Sohling, Ulrich A1 - Ruf, Friedrich A1 - Ulber, Roland T1 - Recycling of spent oil bleaching earth as source of glycerol for the anaerobic production of acetone, butanol, and ethanol with Clostridium diolis and lipolytic Clostridium lundense JF - Engineering in Life Sciences N2 - A major part of edible oil is subjected to bleaching procedures, primarily with minerals applied as adsorbers. Their recycling is currently done either by regaining the oil via organic solvent extraction or by using the spent bleaching earth (SBE) as additive for animal feed, etc. As a new method, the reutilization of the by-product SBE for the microbiologic formation of acetone, butanol, and ethanol (ABE) is presented as proof-of-concept. The SBE was taken from a palm oil cleaning process. The recycling concept is based on the application of lipolytic clostridia strains. Due to considerably long fermentation times, co-fermentation with Candida rugosa and enzymatic hydrolyses of the bound oil with a subsequent clostridia fermentation are shown as alternative routes. Anaerobic fermentations under comparison of different clostridia strains were performed with glycerol media, enzymatically hydrolyzed palm oil and SBE. Solutes, side product compositions and productivities were quantified via HPLC. A successful production of ABE solutes from SBE has been done with a yield of 0.15 g butanol per gram of bound glycerol. Thus, the biotechnological recycling of the waste stream is possible in principle. Inhibition of the substrate suspension has been observed. A chromatographic ion-exchange of substrates increased the biomass concentration. Y1 - 2014 U6 - http://dx.doi.org/10.1002/elsc.201300113 SN - 1618-2863 VL - 14 IS - 4 SP - 425 EP - 432 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Al-Kaidy, Huschyar A1 - Duwe, Anna A1 - Huster, Manuel A1 - Muffler, Kai A1 - Schlegel, Christin A1 - Sieker, Tim A1 - Stadtmüller, Ralf A1 - Tippkötter, Nils A1 - Ulber, Roland T1 - Biotechnologie und Bioverfahrenstechnik – Vom ersten Ullmanns Artikel bis hin zu aktuellen Forschungsthemen JF - Chemie Ingenieur Technik N2 - Biotechnologie und die mit ihr verbundenen technischen Prozesse prägen seit Jahrtausenden die Entwicklung der Menschheit. Ausgehend von empirischen Verfahren, insbesondere zur Herstellung von Lebensmitteln und täglichen Gebrauchsgütern, haben sich diese Disziplinen zu einem der innovativsten Zukunftsfelder entwickelt. Durch das immer detailliertere Verständnis zellulärer Vorgänge können mittlerweile Produktionsstämme gezielt optimiert werden. Im Zusammenspiel mit moderner Prozesstechnik können so eine Vielzahl von Bulk- und Feinchemikalien sowie Pharmazeutika effizient hergestellt werden. In diesem Artikel werden exemplarisch einige der aktuellen Trends vorgestellt. Y1 - 2014 U6 - http://dx.doi.org/10.1002/cite.201400083 SN - 0009-286X VL - 86 IS - 12 SP - 2215 EP - 2225 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Wiesen, Sebastian A1 - Tippkötter, Nils A1 - Muffler, Kai A1 - Suck, Kirstin A1 - Sohling, Ulrich A1 - Ruf, Nils A1 - Ulber, Roland T1 - Adsorptive Vorbehandlung von Rohglycerin für die 1,3-Propandiol Fermentation mit Clostridium diolis JF - Chemie Ingenieur Technik N2 - Bei der Gewinnung von Fettsäuren aus Pflanzenölen, z. B. zur Herstellung von Biopolymeren, oder bei der Biodiesel- und Seifenproduktion, fällt Glycerin als Nebenprodukt an. Bei der Biokonversion dieses Rohstoffes zu 1,3-Propandiol wird der Produktionsorganismus Clostridium diolis durch Verunreinigungen im Rohglycerin gehemmt. Als inhibierende Substanzen konnten freie Fettsäuren identifiziert werden. Mithilfe eines adsorptiven Aufarbeitungsverfahrens ist es gelungen, die Fettsäuren zu entfernen und die Konversionseffizienz zu 1,3-Propandiol zu erhöhen. Y1 - 2014 U6 - http://dx.doi.org/10.1002/cite.201300080 N1 - Englischer Titel: Adsorptive Pretreatment of Crude Glycerol Prior to Fermentation to 1,3-Propanediole by Clostridium Diolis VL - 86 IS - 1-2 SP - 129 EP - 135 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Thiel, Alexander A1 - Tippkötter, Nils A1 - Suck, Kirstin A1 - Sohling, Ulrich A1 - Ruf, Friedrich A1 - Ulber, Roland T1 - New zeolite adsorbents for downstream processing of polyphenols from renewable resources JF - Engineering in Life Sciences N2 - Commercial materials with polyvinylpolypyrrolidone and polymeric amberlites (XAD7HP, XAD16) are commonly used for the adsorptive downstream processing of polyphenols from renewable resources. In this study, beta-zeolite-based adsorbent systems were examined, and their properties were compared to organic resins. Batch adsorption experiments were conducted with synthetic solutions of major polyphenols. Adsorption isotherms and desorption characteristics of individual adsorbent were determined based on these results. Maximum adsorption capacities were calculated using the Langmuir model. For example, the zeolites had capacities up to 203.2 mg/g for ferulic acid. To extend these results to a complex system, additional experiments were performed on rapeseed meal and wheat seed extracts as representative renewable resources. HPLC analysis showed that with 7.5% w/v, which is regarded as the optimum amount of zeolites, zeolites A and B could bind 100% of the major polyphenols as well as release polyphenols at high yields. Additionally, regeneration experiments were performed with isopropyl alcohol at 99°C to evaluate how zeolites regenerate under mild conditions. The results showed only a negligible loss of adsorption capacity and no loss of desorption capacity. In summary, it was concluded that beta-zeolites were promising adsorbents for developing new processes to isolate polyphenols from renewable resources. Y1 - 2013 U6 - http://dx.doi.org/10.1002/elsc.201200188 VL - 13 IS - 3 SP - 239 EP - 246 PB - Wiley CY - Weinheim ER - TY - JOUR A1 - Tippkötter, Nils A1 - Al-Kaidy, Huschyar A1 - Wollny, Steffen A1 - Ulber, Roland T1 - Functionalized magnetizable particles for downstream processing in single-use systems JF - Chemie Ingenieur Technik N2 - Biotechnological downstream processing is usually an elaborate procedure, requiring a multitude of unit operations to isolate the target component. Besides the disadvantageous space-time yield, the risks of cross-contaminations and product loss grow fast with the complexity of the isolation procedure. A significant reduction of unit operations can be achieved by application of magnetic particles, especially if these are functionalized with affinity ligands. As magnetic susceptible materials are highly uncommon in biotechnological processes, target binding and selective separation of such particles from fermentation or reactions broths can be done in a single step. Since the magnetizable particles can be produced from iron salts and low priced polymers, a single-use implementation of these systems is highly conceivable. In this article, the principles of magnetizable particles, their synthesis and functionalization are explained. Furthermore, applications in the area of reaction engineering, microfluidics and downstream processing are discussed focusing on established single-use technologies and development potential. Y1 - 2013 U6 - http://dx.doi.org/10.1002/cite.201200130 VL - 85 IS - 1-2: Special Issue: Single-Use Technology SP - 76 EP - 86 PB - Wiley CY - Weinheim ER - TY - JOUR A1 - Sieker, Tim A1 - Neuner, Andreas A1 - Dimitrova, Darina A1 - Tippkötter, Nils A1 - Muffler, Kai A1 - Bart, Hans-Jörg A1 - Heinzle, Elmar A1 - Ulber, Roland T1 - Ethanol production from grass silage by simultaneous pretreatment, saccharification and fermentation: First steps in the process development JF - Engineering in Life Sciences N2 - Grass silage provides a great potential as renewable feedstock. Two fractions of the grass silage, a press juice and the fiber fraction, were evaluated for their possible use for bioethanol production. Direct production of ethanol from press juice is not possible due to high concentrations of organic acids. For the fiber fraction, alkaline peroxide or enzymatic pretreatment was used, which removes the phenolic acids in the cell wall. In this study, we demonstrate the possibility to integrate the enzymatic pretreatment with a simultaneous saccharification and fermentation to achieve ethanol production from grass silage in a one-process step. Achieved yields were about 53 g ethanol per kg silage with the alkaline peroxide pretreatment and 91 g/kg with the enzymatic pretreatment at concentrations of 8.5 and 14.6 g/L, respectively. Furthermore, it was shown that additional supplementation of the fermentation medium with vitamins, trace elements and nutrient salts is not necessary when the press juice is directly used in the fermentation step. Y1 - 2011 U6 - http://dx.doi.org/10.1002/elsc.201000160 N1 - Special Issue "Bioprocess‐oriented plant design" VL - 11 IS - 4 SP - 436 EP - 442 PB - Wiley CY - Weinheim ER - TY - JOUR A1 - Poth, Sebastian A1 - Monzon, Magaly A1 - Tippkötter, Nils A1 - Ulber, Roland T1 - Lignocellulosic biorefinery: Process integration of hydrolysis and fermentation (SSF process) JF - Holzforschung N2 - The aim of the present work is the process integration and the optimization of the enzymatic hydrolysis of wood and the following fermentation of the products to ethanol. The substrate is a fiber fraction obtained by organosolv pre-treatment of beech wood. For the ethanol production, a co-fermentation by two different yeasts (Saccharomyces cerevisiae and Pachysolen tannophilus) was carried out to convert glucose as well as xylose. Two approaches has been followed: 1. A two step process, in which the hydrolysis of the fiber fraction and the fermentation to product are separated from each other. 2. A process, in which the hydrolysis and the fermentation are carried out in one single process step as simultaneous saccharification and fermentation (SSF). Following the first approach, a yield of about 0.15 g ethanol per gram substrate can be reached. Based on the SSF, one process step can be saved, and additionally, the gained yield can be raised up to 0.3 g ethanol per gram substrate. Y1 - 2011 N1 - 11th EWLP, Hamburg, Germany, August 16–19, 2010 VL - 65 IS - 5 SP - 633 EP - 637 PB - De Gruyter CY - Berlin ER - TY - JOUR A1 - Rösch, C. A1 - Kratz, F. A1 - Hering, T. A1 - Trautmann, S. A1 - Umanskaya, N. A1 - Tippkötter, Nils A1 - Müller-Renno, C.M. A1 - Ulber, R. A1 - Hannig, M. A1 - Ziegler, C. T1 - Albumin-lysozyme interactions: cooperative adsorption on titanium and enzymatic activity JF - Colloids and Surfaces B: Biointerfaces N2 - The interplay of albumin (BSA) and lysozyme (LYZ) adsorbed simultaneously on titanium was analyzed by gel electrophoresis and BCA assay. It was found that BSA and lysozyme adsorb cooperatively. Additionally, the isoelectric point of the respective protein influences the adsorption. Also, the enzymatic activity of lysozyme and amylase (AMY) in mixtures with BSA was considered with respect to a possible influence of protein-protein interaction on enzyme activity. Indeed, an increase of lysozyme activity in the presence of BSA could be observed. In contrast, BSA does not influence the activity of amylase. Y1 - 2016 U6 - http://dx.doi.org/10.1016/j.colsurfb.2016.09.048 VL - 149 IS - 1 SP - 115 EP - 121 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Roth, Jasmine A1 - Tippkötter, Nils T1 - Evaluation of lignocellulosic material for butanol production using enzymatic hydrolysate medium JF - Cellulose Chemistry and Technology N2 - Butanol is a promising gasoline additive and platform chemical that can be readily produced via acetone-butanolethanol (ABE) fermentation from pretreated lignocellulosic materials. This article examines lignocellulosic material from beech wood for ABE fermentation, using Clostridium acetobutylicum. First, the utilization of both C₅₋ (xylose) and C₆₋ (glucose) sugars as sole carbon source was investigated in static cultivation, using serum bottles and synthetic medium. The utilization of pentose sugar resulted in a solvent yield of 0.231 g·g_sugar⁻¹, compared to 0.262 g·g_sugar⁻¹ using hexose. Then, the Organosolv pretreated crude cellulose fibers (CF) were enzymatically decomposed, and the resulting hydrolysate medium was analyzed for inhibiting compounds (furans, organic acids, phenolics) and treated with ionexchangers for detoxification. Batch fermentation in a bioreactor using CF hydrolysate medium resulted in a total solvent yield of 0.20 gABE·g_sugar⁻¹. Y1 - 2016 VL - 50 IS - 3-4 SP - 405 EP - 410 PB - Editura Academiei Romane CY - Bukarest ER - TY - JOUR A1 - Al-Kaidy, Huschyar A1 - Tippkötter, Nils T1 - Superparamagnetic hydrophobic particles as shell material for digital microfluidic droplets and proof-of-principle reaction assessments with immobilized laccase JF - Engineering in Life Sciences N2 - In the field of biotechnology and molecular biology, the use of small liquid volumes has significant advantages. In particular, screening and optimization runs with acceptable amounts of expensive and hardly available catalysts, reagents, or biomolecules are feasible with microfluidic technologies. The presented new microfluidic system is based on the inclusion of small liquid volumes by a protective shell of magnetizable microparticles. Hereby, discrete aqueous microreactor drops with volumes of 1–30 μL can be formed on a simple planar surface. A digital movement and manipulation of the microreactor is performed by overlapping magnetic forces. The magnetic forces are generated by an electrical coil matrix positioned below a glass plate. With the new platform technology, several discrete reaction compartments can be moved simultaneously on one surface. Due to the magnetic fields, the reactors can even be merged to initiate reactions by mixing or positioned above surface-immobilized catalysts and then opened by magnetic force. Comparative synthesis routes of the magnetizable shell particles and superhydrophobic glass slides including their performance and stability with the reaction platform are described. The influence of diffusive mass transport during the catalyzed reaction is discussed by evaluation finite element model of the microreactor. Furthermore, a first model dye reaction of the enzyme laccase has been established. Y1 - 2016 U6 - http://dx.doi.org/10.1002/elsc.201400124 VL - 16 IS - 3 SP - 222 EP - 230 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Al-Kaidy, Huschyar A1 - Duwe, Anna A1 - Huster, Manuel A1 - Muffler, Kai A1 - Schlegel, Christin A1 - Tim, Sieker A1 - Stadtmüller, Ralf A1 - Tippkötter, Nils A1 - Ulber, Roland T1 - Biotechnology and bioprocess engineering – from the first ullmann's article to recent trends JF - ChemBioEng Reviews N2 - For several thousand years, biotechnology and its associated technical processes have had a great impact on the development of mankind. Based on empirical methods, in particular for the production of foodstuffs and daily commodities, these disciplines have become one of the most innovative future issues. Due to the increasing detailed understanding of cellular processes, production strains can now be optimized. In combination with modern bioprocesses, a variety of bulk and fine chemicals as well as pharmaceuticals can be produced efficiently. In this article, some of the current trends in biotechnology are discussed. Y1 - 2015 U6 - http://dx.doi.org/10.1002/cben.201500008 VL - 2 IS - 3 SP - 175 EP - 184 PB - Wiley CY - Weinheim ER - TY - JOUR A1 - Thiel, Alexander A1 - Muffler, Kai A1 - Tippkötter, Nils A1 - Suck, Kirstin A1 - Sohling, Ulrich A1 - Hruschka, Steffen M. A1 - Ulber, Roland T1 - A novel integrated downstream processing approach to recover sinapic acid, phytic acid and proteins from rapeseed meal JF - Journal of Chemical Technology and Biotechnology N2 - BACKGROUND Currently, several techniques exist for the downstream processing of protein, phytic acid and sinapic acid from rapeseed and rapeseed meal, but no technique has been developed to separate all of the components in one process. In this work, two new downstream processing strategies focusing on recovering sinapic acid, phytic acid and protein from rapeseed meal were established. RESULTS The sinapic acid content was enhanced by a factor of 4.5 with one method and 5.1 with the other. The isolation of sinapic acid was accomplished using a zeolite-based adsorbent with high adsorptive and optimal desorption characteristics. Phytic acid was isolated using the anion-exchange resin Purolite A200®. In addition, the processes resulted in two separated protein fractions. The ratios of globulin and albumin ratio to the total protein were 59.2% and 40.1%, respectively. The steps were then combined in two different ways: (a) a ‘sequential process’ using the zeolite and A200 in batch processes; and (b) a ‘parallel process’ using only A200 in a chromatographic system to separate all of the compounds. CONCLUSIONS It can be concluded that isolation of all three components was possible in both processes. These could enhance the added value of current processes using rapeseed meal as a protein source. © 2015 Society of Chemical Industry Y1 - 2015 U6 - http://dx.doi.org/10.1002/jctb.4664 VL - 90 IS - 11 SP - 1999 EP - 2006 PB - Wiley CY - Weinheim ER - TY - JOUR A1 - Schumann, Christiane A1 - Rogin, Sabine A1 - Schneider, Horst A1 - Tippkötter, Nils A1 - Oster, Jürgen A1 - Kampeis, Percy T1 - Simultane Atline-Quantifizierung von Magnetpartikeln und Mikroorganismen bei einer HGMS-Filtration JF - Chemie Ingenieur Technik N2 - Es wird eine neue Atline-Messmethode vorgestellt, mit der während einer Hochgradienten-Magnetseparation (HGMS)-Filtration eine simultane Quantifizierung von Magnetpartikeln und Mikroorganismen im Filtrat vorgenommen werden kann. Dabei gelingt die Quantifizierung signifikant besser als mit bisher verwendeten Messmethoden. Mit dieser Methode ist es möglich, die Trennleistung einer HGMS-Filtration zu bestimmen und einen Filterdurchbruch durch Konzentrationsanstiege im Bereich einiger µg L−1 von Magnetpartikeln im Filtrat frühzeitig zu detektieren, ohne dass nennenswerte Partikelmengen verloren gehen. Y1 - 2015 U6 - http://dx.doi.org/10.1002/cite.201300158 N1 - Englischer Titel: Simultaneous Atline Quantification of Magnetic Particles and Microorganisms in the HGMS Filtration VL - 87 IS - 1-2 SP - 137 EP - 149 ER - TY - JOUR A1 - Thiel, Alexander A1 - Muffler, Kai A1 - Tippkötter, Nils A1 - Suck, Kirstin A1 - Sohling, Ulrich A1 - Ruf, Friedrich A1 - Ulber, Roland T1 - Aufarbeitung von Polyphenolen aus Weizen mittels Zeolithen am Beispiel der Ferulasäure JF - Chemie IngenieurTechnik N2 - Aufarbeitung von Polyphenolen aus Weizenmittels Zeolithen am Beispiel der Ferulasa¨ ureAlexander Thiel1, Kai Muffler1, Nils Tippko¨ tter1, Kirstin Suck2, Ulrich Sohling2, Friedrich Ruf3und Roland Ulber1,*DOI: 10.1002/cite.201400031Bei der Ferulasa¨ure handelt es sich um einen Wertstoff, der aus Weizen gewonnen und in der Lebensmittel- und Pharma-industrie eingesetzt werden kann. Der Einsatz von Weizen als nachwachsende Rohstoffquelle ist allerdings nur dann wirt-schaftlich durchfu¨hrbar, wenn eine Prozessintegration in die bestehenden industriellen Verfahren gewa¨hrleistet oder einedirekte Konkurrenz zur Mehl- und Sta¨rkeindustrie vermieden werden kann. In diesem Artikel wird ein Verfahren aufge-zeigt, welches hohe Ausbeuten ermo¨glicht und eine Konkurrenz zu bestehenden Verwertungspfaden vermeidet. Y1 - 2015 U6 - http://dx.doi.org/10.1002/cite.201400031 N1 - Englischer Titel: Downstream Processing of Polyphenols from Wheat by Zeolites using the Example of Ferulic Acid VL - 87 IS - 1-2 SP - 128 EP - 136 PB - Wiley CY - Weinheim ER - TY - JOUR A1 - Wiesen, Sebastian A1 - Tippkötter, Nils A1 - Muffler, Kai A1 - Suck, Kirstin A1 - Sohling, Ulrich A1 - Ruf, Friedrich A1 - Ulber, Roland T1 - Adsorption of fatty acids to layered double hydroxides in aqueous systems JF - Adsorption N2 - Due to their anion exchange characteristics, layered double hydroxides (LDHs) are suitable for the detoxification of aqueous, fatty acid containing fermentation substrates. The aim of this study is to examine the adsorption mechanism, using crude glycerol from plant oil esterification as a model system. Changes in the intercalation structure in relation to the amount of fatty acids adsorbed are monitored by X-ray diffraction and infra-red spectroscopy. Additionally, calcination of LDH is investigated in order to increase the binding capacity for fatty acids. Our data propose that, at ambient temperature, fatty acids can be bound to the hydrotalcite by adsorption or in addition by intercalation, depending on fatty acid concentration. The adsorption of fatty acids from crude glycerol shows a BET-like behavior. Above a fatty acid concentration of 3.5 g L−1, intercalation of fatty acids can be shown by the appearance of an increased interlayer spacing. This observation suggests a two phase adsorption process. Calcination of LDHs allows increasing the binding capacity for fatty acids by more than six times, mainly by reduction of structural CO32−. Y1 - 2015 VL - 21 IS - 6-7 SP - 459 EP - 466 PB - Springer CY - Berlin ER - TY - JOUR A1 - Tippkötter, Nils A1 - Duwe, Anna-Maria A1 - Wiesen, Sebastian A1 - Sieker, Tim A1 - Ulber, Roland T1 - Enzymatic hydrolysis of beech wood lignocellulose at high solid contents and its utilization as substrate for the production of biobutanol and dicarboxylic acids JF - Bioresource Technology N2 - The development of a cost-effective hydrolysis for crude cellulose is an essential part of biorefinery developments. To establish such high solid hydrolysis, a new solid state reactor with static mixing is used. However, concentrations >10% (w/w) cause a rate and yield reduction of enzymatic hydrolysis. By optimizing the synergetic activity of cellulolytic enzymes at solid concentrations of 9%, 17% and 23% (w/w) of crude Organosolv cellulose, glucose concentrations of 57, 113 and 152 g L⁻¹ are reached. However, the glucose yield decreases from 0.81 to 0.72gg⁻¹ at 17% (w/w). Optimal conditions for hydrolysis scale-up under minimal enzyme addition are identified. As result, at 23% (w/w) crude cellulose the glucose yield increases from 0.29 to 0.49gg⁻¹. As proof of its applicability, biobutanol, succinic and itaconic acid are produced with the crude hydrolysate. The potential of the substrate is proven e.g. by a high butanol yield of 0.33gg⁻¹. Y1 - 2014 U6 - http://dx.doi.org/10.1016/j.biortech.2014.06.052 VL - 167 SP - 447 EP - 455 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Muschallik, Lukas A1 - Molinnus, Denise A1 - Bongaerts, Johannes A1 - Pohl, Martina A1 - Wagner, Torsten A1 - Schöning, Michael Josef A1 - Siegert, Petra A1 - Selmer, Thorsten T1 - (R,R)-Butane-2,3-diol Dehydrogenase from Bacillus clausii DSM 8716T: Cloning and Expression of the bdhA-Gene, and Initial Characterization of Enzyme JF - Journal of Biotechnology N2 - The gene encoding a putative (R,R)-butane-2,3-diol dehydrogenase (bdhA) from Bacillus clausii DSM 8716T was isolated, sequenced and expressed in Escherichia coli. The amino acid sequence of the encoded protein is only distantly related to previously studied enzymes (identity 33–43%) and exhibited some uncharted peculiarities. An N-terminally StrepII-tagged enzyme variant was purified and initially characterized. The isolated enzyme catalyzed the (R)-specific oxidation of (R,R)- and meso-butane-2,3-diol to (R)- and (S)-acetoin with specific activities of 12 U/mg and 23 U/mg, respectively. Likewise, racemic acetoin was reduced with a specific activity of up to 115 U/mg yielding a mixture of (R,R)- and meso-butane-2,3-diol, while the enzyme reduced butane-2,3-dione (Vmax 74 U/mg) solely to (R,R)-butane-2,3-diol via (R)-acetoin. For these reactions only activity with the co-substrates NADH/NAD+ was observed. The enzyme accepted a selection of vicinal diketones, α-hydroxy ketones and vicinal diols as alternative substrates. Although the physiological function of the enzyme in B. clausii remains elusive, the data presented herein clearly demonstrates that the encoded enzyme is a genuine (R,R)-butane-2,3-diol dehydrogenase with potential for applications in biocatalysis and sensor development. Y1 - 2017 U6 - http://dx.doi.org/10.1016/j.jbiotec.2017.07.020 SN - 0168-1656 VL - 258 SP - 41 EP - 50 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Graf, Alain-Michel A1 - Steinhof, Rafael A1 - Lotz, Martin A1 - Tippkötter, Nils A1 - Kasper, Cornelia A1 - Beutel, Sascha A1 - Ulber, Roland T1 - Downstream-Processing mit Membranadsorbern zur Isolierung nativer Proteinfraktionen aus Kartoffelfruchtwasser JF - Chemie Ingenieur Technik N2 - Bei der Stärkeproduktion entstehendes Kartoffelfruchtwasser besitzt mit 2 – 3 % einen hohen Anteil an ernährungsphysiologisch interessanten Proteinen. Die industrielle Gewinnung dieser Proteinfracht liefert jedoch lediglich ein minderwertiges, denaturiertes Produkt. Mit Hilfe der Membranadsorber-Technologie lassen sich aus Kartoffelfruchtwasser unter milden Reaktionsbedingungen native bioaktive Proteinfraktionen gewinnen. Geeignete Trennbedingungen wurden im Labormaßstab entwickelt und in den Technikumsmaßstab übertragen. An Anionenaustauscher-Membranadsorbern mit einer Membranfläche von 10 000 cm2 wurde eine Patatinhaltige Fraktion (44 kDa) mit Bindungskapazitäten von 0,37 mg/cm2 isoliert. Eine niedermolekulare Proteinfraktion mit Protease-Inhibitoren konnte durch Kationenaustauscher-Membranadsorber mit Bindungskapazitäten von 1,00 mg/cm2 gewonnen werden. Sie ist für verschiedenste Applikationen in der pharmazeutischen, kosmetischen und der Nahrungsmittelindustrie interessant z. B. für Appetitzügler oder muskelaufbauende Proteinpräparate. Der Aufreinigung der nativen Proteinfraktionen durch Ultra-/Diafiltration schließt sich die Konfektionierung durch Sprühtrocknung an. Die bioanalytische Charakterisierung der Produkte belegt die Reinheit und die enzymatische Aktivität sowie die Abreicherung von Störkomponenten wie Glykoalkaloide und Polyphenoloxidasen. Y1 - 2009 U6 - http://dx.doi.org/10.1002/cite.200800139 VL - 81 IS - 3 SP - 267 EP - 274 PB - Wiley CY - Weinheim ER - TY - JOUR A1 - Tippkötter, Nils A1 - Deterding, A. A1 - Ulber, Roland T1 - Determination of acetic acid in fermentation broth by gas-diffusion technique JF - Engineering in Life Sciences N2 - Due to the interfering effects of acetic acid in many fermentation processes, a gas-diffusion technique was developed for the online determination of acetic acid. The measurements were accomplished with a flow diffusion analysis (FDA) unit from the TRACE Analytics GmbH, Braunschweig, Germany. The diffusion analysis is based on the UV-absorbance of acetic acid at 205 nm. The measurement was achieved by the separation of an acceptor and a carrier stream (acidified fermentation broth) using a gas permeable polytetrafluoroethylene (PTFE) membrane, whereby broth constituents that would otherwise disturb the UV-measurement of acetic acid, are held back efficiently. Merely, the fermentation by-products, e.g. formic acid, is capable of diffusing through the membrane. While formic acid can disturb the measurement, carbon dioxide does not absorb at 205 nm. The method operates with time-dependent sample enrichment. During the analysis, a small volume of the acceptor stream is stopped for a defined time interval in the acceptor chamber. During this period, the gaseous acetic acid diffuses through the membrane and is enriched in the acceptor chamber. Subsequently after the enrichment, the acceptor stream flows through a UV-detector. The intensity of the signal is proportional to the acetic acid concentration. Online measurements in bioreactors via a sterile filtration probe have been accomplished. A linear calibration in the range of 0.5–5.0 g/L acetic acid with a relative standard deviation of <5 % was obtained. A sampling rate of 8 samples per hour was possible. The system was applied for the determination of acetic acid in E. coli fermentation broth. The instrument is easy to clean, very user-friendly and does not require any toxic or expensive reagents. Y1 - 2008 U6 - http://dx.doi.org/10.1002/elsc.200820227 VL - 8 IS - 1, Special Issue: Technical Systems for the Use in Life Sciences SP - 62 EP - 67 ER - TY - JOUR A1 - Kappler-Tanudyaya, Nathalie A1 - Schmitt, Heike A1 - Tippkötter, Nils A1 - Meyer, Lina A1 - Lenzen, Sigurd A1 - Ulber, Roland T1 - Combination of biotransformation and chromatography for the isolation and purification of mannoheptulose JF - Biotechnology Journal N2 - Mannoheptulose is a seven-carbon sugar. It is an inhibitor of glucose-induced insulin secretion due to its ability to selectively inhibit the enzyme glucokinase. An improved procedure for mannoheptulose isolation from avocados is described in this study (based upon the original method by La Forge). The study focuses on the combination of biotransformation and downstream processing (preparative chromatography) as an efficient method to produce a pure extract of mannoheptulose. The experiments were divided into two major phases. In the first phase, several methods and parameters were compared to optimize the mannoheptulose extraction with respect to efficiency and purity. In the second phase, a mass balance of mannoheptulose over the whole extraction process was undertaken to estimate the yield and efficiency of the total extraction process. The combination of biotransformation and preparative chromatography allowed the production of a pure mannoheptulose extract. In a biological test, the sugar inhibited the glucokinase enzyme activity efficiently. Y1 - 2007 U6 - http://dx.doi.org/10.1002/biot.200700004 SN - 1860-7314 VL - 2 IS - 6 SP - 692 EP - 699 ER - TY - CHAP A1 - Tippkötter, Nils ED - Kaltschmidt, Martin T1 - Grundlagen der bio-chemischen Umwandlung T2 - Energie aus Biomasse : Grundlagen, Techniken und Verfahren Y1 - 2016 SN - 978-3-662-47437-2 (Print) SN - 978-3-662-47438-9 (Online) U6 - http://dx.doi.org/10.1007/978-3-662-47438-9 SP - 1447 EP - 1500 PB - Springer Vieweg CY - Berlin ; Heidelberg ET - 3., aktualisierte, erweiterte Auflage ER - TY - CHAP A1 - Hahn, Thomas A1 - Kelly, Svenja A1 - Muffler, Kai A1 - Tippkötter, Nils A1 - Ulber, Roland ED - Hans-Jörg, Bart ED - Pilz, Stephan T1 - Extraction of lignocellulose and algae for the production of bulk and fine chemicals T2 - Industrial scale natural products extraction Y1 - 2011 SN - 978-3-527-32504-7 (Print) SN - 978-3-527-63512-2 (Online) U6 - http://dx.doi.org/10.1002/9783527635122 SP - 221 EP - 245 PB - Wiley-VCH CY - Weinheim ER - TY - CHAP A1 - Muffler, Kai A1 - Poth, Sabastian A1 - Sieker, Tim A1 - Tippkötter, Nils A1 - Ulber, Roland A1 - Sell, Dieter ED - Moo-Young, Murray T1 - Bio-feedstocks T2 - Comprehensive biotechnology : principles and practices in industry, agcriculture, medicine and the environment. Volume 2: Engineering fundamentals of biotechnology Y1 - 2011 SN - 978-0-444-53352-4 U6 - http://dx.doi.org/10.1016/B978-0-08-088504-9.00088-X SP - 93 EP - 101 PB - Elsevier CY - Amsterdam ET - 2. edition ER - TY - JOUR A1 - Röhlen, Desiree A1 - Pilas, Johanna A1 - Schöning, Michael Josef A1 - Selmer, Thorsten T1 - Development of an amperometric biosensor platform for the combined determination of l-Malic, Fumaric, and l-Aspartic acid JF - Applied Biochemistry and Biotechnology N2 - Three amperometric biosensors have been developed for the detection of L-malic acid, fumaric acid, and L -aspartic acid, all based on the combination of a malate-specific dehydrogenase (MDH, EC 1.1.1.37) and diaphorase (DIA, EC 1.8.1.4). The stepwise expansion of the malate platform with the enzymes fumarate hydratase (FH, EC 4.2.1.2) and aspartate ammonia-lyase (ASPA, EC 4.3.1.1) resulted in multi-enzyme reaction cascades and, thus, augmentation of the substrate spectrum of the sensors. Electrochemical measurements were carried out in presence of the cofactor β-nicotinamide adenine dinucleotide (NAD+) and the redox mediator hexacyanoferrate (III) (HCFIII). The amperometric detection is mediated by oxidation of hexacyanoferrate (II) (HCFII) at an applied potential of + 0.3 V vs. Ag/AgCl. For each biosensor, optimum working conditions were defined by adjustment of cofactor concentrations, buffer pH, and immobilization procedure. Under these improved conditions, amperometric responses were linear up to 3.0 mM for L-malate and fumarate, respectively, with a corresponding sensitivity of 0.7 μA mM−1 (L-malate biosensor) and 0.4 μA mM−1 (fumarate biosensor). The L-aspartate detection system displayed a linear range of 1.0–10.0 mM with a sensitivity of 0.09 μA mM−1. The sensor characteristics suggest that the developed platform provides a promising method for the detection and differentiation of the three substrates. Y1 - 2017 U6 - http://dx.doi.org/10.1007/s12010-017-2578-1 SN - 1559-0291 VL - 183 SP - 566 EP - 581 PB - Springer CY - Berlin ER - TY - JOUR A1 - Pilas, Johanna A1 - Yazici, Yasemen A1 - Selmer, Thorsten A1 - Keusgen, Michael A1 - Schöning, Michael Josef T1 - Optimization of an amperometric biosensor array for simultaneous measurement of ethanol, formate, d- and l-lactate JF - Electrochimica Acta N2 - The immobilization of NAD+-dependent dehydrogenases, in combination with a diaphorase, enables the facile development of multiparametric sensing devices. In this work, an amperometric biosensor array for simultaneous determination of ethanol, formate, d- and l-lactate is presented. Enzyme immobilization on platinum thin-film electrodes was realized by chemical cross-linking with glutaraldehyde. The optimization of the sensor performance was investigated with regard to enzyme loading, glutaraldehyde concentration, pH, cofactor concentration and temperature. Under optimal working conditions (potassium phosphate buffer with pH 7.5, 2.5 mmol L-1 NAD+, 2.0 mmol L-1 ferricyanide, 25 °C and 0.4% glutaraldehyde) the linear working range and sensitivity of the four sensor elements was improved. Simultaneous and cross-talk free measurements of four different metabolic parameters were performed successfully. The reliable analytical performance of the biosensor array was demonstrated by application in a clarified sample of inoculum sludge. Thereby, a promising approach for on-site monitoring of fermentation processes is provided. KW - Simultaneous determination KW - Enzymatic biosensor KW - Diaphorase KW - Dehydrogenase Y1 - 2017 U6 - http://dx.doi.org/10.1016/j.electacta.2017.07.119 SN - 0013-4686 VL - 251 SP - 256 EP - 262 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Seifarth, Volker A1 - Grosse, Joachim O. A1 - Grossmann, Matthias A1 - Janke, Heinz Peter A1 - Arndt, Patrick A1 - Koch, Sabine A1 - Epple, Matthias A1 - Artmann, Gerhard A1 - Temiz Artmann, Aysegül T1 - Mechanical induction of bi-directional orientation of primary porcine bladder smooth muscle cells in tubular fibrin-poly(vinylidene fluoride) scaffolds for ureteral and urethral repair using cyclic and focal balloon catheter stimulation JF - Journal of Biomaterials Applications Y1 - 2017 U6 - http://dx.doi.org/10.1177/0885328217723178 SN - 1530-8022 VL - 32 IS - 3 SP - 321 EP - 330 PB - Sage CY - London ER - TY - CHAP A1 - Muffler, Kai A1 - Tippkötter, Nils A1 - Ulber, Roland ED - Timmis, Kenneth N. T1 - Chemical feedstocks and fine chemicals from other substrates T2 - Handbook of hydrocarbon and lipid microbiology. Volume 4: Consequences of microbial interactions with hydrocarbons, oils and lipids. - (Springer reference) Y1 - 2010 SN - 978-3-540-77588-1 U6 - http://dx.doi.org/10.1007%2F978-3-540-77587-4_214 SP - 2891 EP - 2902 PB - Springer CY - Berlin [u.a.] ER - TY - BOOK A1 - Tippkötter, Nils T1 - Reaktionssysteme zur Aufarbeitung und Umsetzung nachwachsender Rohstoffe : Einsatz chromatographischer Verfahren sowie Membran- und Festbettreaktoren zur Verarbeitung von Molke, Stärke und Cellulose Y1 - 2010 SN - 978-3-8325-2717-4 N1 - Kaiserslautern, Technische Universität, Dissertation, 2010 PB - Logos-Verlag CY - Berlin ER - TY - CHAP A1 - Poth, Sebastian A1 - Monzon, Magaly A1 - Tippkötter, Nils A1 - Ulber, Roland T1 - Lignocellulosic biorefinery : process integration of hydrolysis and fermentation T2 - Proceedings / 11th European Workshop on Lignocellulosics and Pulp : August 16 - 19, 2010, Hamburg, Germany Y1 - 2010 SP - 65 EP - 68 PB - vTi CY - Hamburg ER - TY - JOUR A1 - Tippkötter, Nils A1 - Wollny, S. A1 - Kampeis, P. A1 - Oster, J. A1 - Schneider, H. A1 - Ulber, R. T1 - Magnetseparation von Proteinen : Separation von Zielmolekülen durch hochselektive Aptamere JF - GIT Labor-Fachzeitschrift N2 - Durch die Kombination von Oligonukleotid-Liganden (Aptameren) hoher Bindungsaffinitäten mit hochselektiv abtrennbaren magnetisierbaren Mikropartikeln wird eine einstufige Separation von Zielmolekülen aus mikrobiologischen Produktionsansätzen möglich. Die Aptamere werden hierfür reversibel auf den Partikeloberflächen gebunden und für die spezifische Isolierung von Bioprodukten eingesetzt. Die Abtrennung der beladenen Partikel erfolgt durch einen neuen Rotor-Stator-Separator mit Hochgradient-Magnetfeld. Y1 - 2011 VL - 55 IS - 10 SP - 666 PB - Wiley CY - Weinheim ER - TY - JOUR A1 - Sieker, Tim A1 - Ulber, Roland A1 - Dimitrova, Darina A1 - Bart, Hans-Jörg A1 - Neuner, Andreas A1 - Heinzle, Elmar A1 - Tippkötter, Nils T1 - Silage : Fermentationsrohstoff für die chemische Industrie? JF - labor&more N2 - In Anbetracht des zu erwartenden Rückgangs der Verfügbarkeit fossiler Rohstoffe müssen nicht nur für den Energiesektor, sondern auch für die Herstellung industrieller Produkte alternative Rohstoffe gefunden werden. Ein Beispiel für einen nicht in Nahrungsmittelkonkurrenz stehenden nachwachsenden Rohstoff ist grüne Biomasse wie Gras und Klee. Diese lassen sich in Deutschland auf großen Flächen anbauen und enthalten eine Vielzahl potenzieller Substrate für Fermentationen. Y1 - 2009 IS - 2 SP - 44 EP - 45 ER - TY - JOUR A1 - Al-Kaidy, Huschyar A1 - Kuthan, Kai A1 - Hering, Thomas A1 - Tippkötter, Nils T1 - Aqueous droplets used as enzymatic microreactors and their electromagnetic actuation JF - Journal of Visualized Experiments N2 - For the successful implementation of microfluidic reaction systems, such as PCR and electrophoresis, the movement of small liquid volumes is essential. In conventional lab-on-a-chip-platforms, solvents and samples are passed through defined microfluidic channels with complex flow control installations. The droplet actuation platform presented here is a promising alternative. With it, it is possible to move a liquid drop (microreactor) on a planar surface of a reaction platform (lab-in-a-drop). The actuation of microreactors on the hydrophobic surface of the platform is based on the use of magnetic forces acting on the outer shell of the liquid drops which is made of a thin layer of superhydrophobic magnetite particles. The hydrophobic surface of the platform is needed to avoid any contact between the liquid core and the surface to allow a smooth movement of the microreactor. On the platform, one or more microreactors with volumes of 10 µL can be positioned and moved simultaneously. The platform itself consists of a 3 x 3 matrix of electrical double coils which accommodate either neodymium or iron cores. The magnetic field gradients are automatically controlled. By variation of the magnetic field gradients, the microreactors' magnetic hydrophobic shell can be manipulated automatically to move the microreactor or open the shell reversibly. Reactions of substrates and corresponding enzymes can be initiated by merging the microreactors or bringing them into contact with surface immobilized catalysts. Y1 - 2016 U6 - http://dx.doi.org/10.3791/54643 SN - 1940-087X IS - Issue 126 ER - TY - JOUR A1 - Wulfhorst, Helene A1 - Duwe, Anna-Maria A1 - Merseburg, Johannes A1 - Tippkötter, Nils T1 - Compositional analysis of pretreated (beech) wood using differential scanning calorimetry and multivariate data analysis JF - Tetrahedron N2 - The composition of plant biomass varies depending on the feedstock and pre-treatment conditions and influences its processing in biorefineries. In order to ensure optimal process conditions, the quantitative proportion of the main polymeric components of the pre-treated biomass has to be determined. Current standard procedures for biomass compositional analysis are complex, the measurements are afflicted with errors and therefore often not comparable. Hence, new powerful analytical methods are urgently required to characterize biomass. In this contribution, Differential Scanning Calorimetry (DSC) was applied in combination with multivariate data analysis (MVA) to detect the cellulose content of the plant biomass pretreated by Liquid Hot Water (LHW) and Organosolv processes under various conditions. Unlike conventional techniques, the developed analytic method enables the accurate quantification of monosaccharide content of the plant biomass without any previous sample preparation. It is easy to handle and avoids errors in sample preparation. Y1 - 2016 U6 - http://dx.doi.org/10.1016/j.tet.2016.04.029 VL - 72 IS - 46 SP - 7329 EP - 7334 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Tippkötter, Nils A1 - Stückmann, Henning A1 - Kroll, Stephen A1 - Winkelmann, Gunda A1 - Noack, Udo A1 - Scheper, Thomas A1 - Ulber, Roland T1 - A semi-quantitative dipstick assay for microcystin JF - Analytical and Bioanalytical Chemistry N2 - An immunochromatographic lateral flow dipstick assay for the fast detection of microcystin-LR was developed. Colloid gold particles with diameters of 40 nm were used as red-colored antibody labels for the visual detection of the antigen. The new dipstick sensor is capable of detecting down to 5 µg·l−1 (ppb; total inversion of the color signal) or 1 ppb (observation of color grading) of microcystin-LR. The course of the labeling reaction was observed via spectrometric wave shifts caused by the change of particle size during the binding of antibodies. Different stabilizing reagents showed that especially bovine serum albumin (BSA) and casein increase the assays sensitivity and the conjugate stability. Performance of the dipsticks was quantified by pattern processing of capture zone CCD images. Storage stability of dipsticks and conjugate suspensions over 115 days under different conditions were monitored. The ready-to-use dipsticks were successfully tested with microcystin-LR-spiked samples of outdoor drinking- and salt water and applied to the tissue of microcystin-fed mussels. Y1 - 2009 U6 - http://dx.doi.org/10.1007/s00216-009-2750-8 SN - 1618-2650 VL - 394 IS - 3 SP - 863 EP - 869 PB - springer CY - Berlin ER - TY - JOUR A1 - Ulber, Roland A1 - Tippkötter, Nils T1 - Nitratfreie Molke JF - Rundschau für Fleischhygiene und Lebensmittelüberwachung Y1 - 2009 IS - 4 SP - 150 EP - 152 ER - TY - JOUR A1 - Breuer, Lars A1 - Mang, Thomas A1 - Schöning, Michael Josef A1 - Thoelen, Ronald A1 - Wagner, Torsten T1 - Investigation of the spatial resolution of a laser-based stimulation process for light-addressable hydrogels with incorporated graphene oxide by means of IR thermography JF - Sensors and Actuators A: Physical Y1 - 2017 U6 - http://dx.doi.org/10.1016/j.sna.2017.11.031 SN - 0924-4247 VL - 268 SP - 126 EP - 132 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Demmer, Julius K. A1 - Chowdhury, Nilanjan Pal A1 - Selmer, Thorsten A1 - Ermler, Ulrich A1 - Buckel, Wolfgang T1 - The semiquinone swing in the bifurcating electron transferring flavoprotein/butyryl-CoA dehydrogenase complex from Clostridium difficile JF - Nature Communications Y1 - 2017 U6 - http://dx.doi.org/10.1038/s41467-017-01746-3 SN - 2041-1723 N1 - Article number 1577 VL - 8 IS - 1 SP - 1 EP - 10 ER - TY - JOUR A1 - Werkhausen, Amelie A1 - Albracht, Kirsten A1 - Cronin, Neil J. A1 - Meier, Rahel A1 - Mojsen-Moeller, Jens A1 - Seynnes, Olivier R. T1 - Modulation of muscle-tendon interaction in the human triceps surae during an energy dissipation task JF - Journal of Experimental Biology Y1 - 2017 U6 - http://dx.doi.org/10.1242/jeb.164111 SN - 0022-0949 VL - 220 IS - 22 SP - 4141 EP - 4149 ER - TY - CHAP A1 - Engel, M. A1 - Thieringer, J. A1 - Tippkötter, Nils T1 - Microbial electrosynthesis for sustainable biobutanol production T2 - New frontiers of biotech-processes (Himmelfahrtstagung) : 02-04 May 2016, Rhein-Mosel-Halle, Koblenz/Germany Y1 - 2016 SP - 77 EP - 78 PB - DECHEMA CY - Frankfurt am Main ER - TY - CHAP A1 - Hering, T. A1 - Ulber, Roland A1 - Tippkötter, Nils T1 - Development of a screening system for antimicrobial surfaces T2 - New frontiers of biotech-processes (Himmelfahrtstagung) : 02-04 May 2016, Rhein-Mosel-Halle, Koblenz/Germany Y1 - 2016 SP - 129 PB - DECHEMA CY - Frankfurt am Main ER - TY - CHAP A1 - Roth, J. A1 - Möhring, S. A1 - Tippkötter, Nils T1 - Characterization and evaluation of lignocellulosic biomass 130 hydrolysates for ABE fermentation T2 - New frontiers of biotech-processes (Himmelfahrtstagung) : 02-04 May 2016, Rhein-Mosel-Halle, Koblenz/Germany Y1 - 2016 SP - 130 PB - DECHEMA CY - Frankfurt am Main ER - TY - CHAP A1 - Möhring, S. A1 - Wulfhorst, H. A1 - Roth, J. A1 - Tippkötter, Nils T1 - Pretreatment strategies for lignocellulosic biomass T2 - New frontiers of biotech-processes (Himmelfahrtstagung) : 02-04 May 2016, Rhein-Mosel-Halle, Koblenz/Germany Y1 - 2016 SP - 131 PB - DECHEMA CY - Frankfurt am Main ER - TY - CHAP A1 - Capitain, C. A1 - Hering, T. A1 - Tippkötter, Nils A1 - Ulber, R. T1 - Enzymatic polymerization of lignin model compounds and solubilized lignin in an aqueous ethanol extract T2 - New frontiers of biotech-processes (Himmelfahrtstagung) : 02-04 May 2016, Rhein-Mosel-Halle, Koblenz/Germany Y1 - 2016 SP - 151 EP - 152 PB - DECHEMA CY - Frankfurt am Main ER - TY - CHAP A1 - Wulfhorst, H. A1 - Duwe, A. A1 - Möhring, S. A1 - Jurca, O. A1 - Tippkötter, Nils T1 - Analysis of pretreated biomass by differential scanning 132 calorimetry and multivariate data analysis T2 - New frontiers of biotech-processes (Himmelfahrtstagung) : 02-04 May 2016, Rhein-Mosel-Halle, Koblenz/Germany Y1 - 2016 SP - 132 PB - DECHEMA CY - Frankfurt am Main ER - TY - CHAP A1 - Deterding, A. A1 - Tippkötter, Nils A1 - Ulber, R. ED - Beckmann, Dieter T1 - Online-Essigsäureanalytik in Fermentationsbrühen mittels Fließdiffusionstechnik (FDT) T2 - Technische Systeme für Biotechnologie und Umwelt : 13. Heiligenstädter Kolloquium, Heilbad Heiligenstadt, 25.09. - 27.09.2006 Y1 - 2006 SN - 978-3-00-018621-9 SP - 273 EP - 280 CY - Heiligenstadt ER - TY - CHAP A1 - Tippkötter, Nils A1 - Schünemann, V. A1 - Christmann, R. A1 - Pasteur, A. A1 - Schweizer, J. A1 - Ulber, R. ED - Beckmann, Dieter T1 - Bioaffinity Layering magnetisierbarer Mikro- und Nanopartikel T2 - Technische Systeme für die Lebenswissenschaften : 14. Heiligenstädter Kolloquium, Heilbad Heiligenstadt, 22.09. - 24.09.2008 Y1 - 2008 SN - 978-3-00-025695-0 SP - 97 EP - 98 CY - Heiligenstadt ER - TY - CHAP A1 - Sieker, T. A1 - Duwe, A. A1 - Poth, S. A1 - Tippkötter, Nils A1 - Ulber, R. T1 - Herstellung von Itaconsäure aus Buchenholzhydrolysaten T2 - Kurzfassungsband / GVC-DECHEMA Vortrags- und Diskussionstagung Biopharmazeutische Produktion : 14. - 16. Mai 2012. Konzerthaus Freibung Y1 - 2012 SP - 57 PB - DECHEMA CY - Frankfurt, M. ER - TY - PAT A1 - Al-Kaidy, Huschyar A1 - Tippkötter, Nils A1 - Ulber, Roland T1 - Vorrichtung und Verfahren zur Bestimmung des Kontaktwinkels eines flüssigen oder mit Flüssigkeit gefüllten Körpers [Offenlegungsschrift] N2 - Die vorliegende Erfindung betrifft eine Vorrichtung und ein Verfahren zur Bestimmung des Kontaktwinkels eines flüssigen oder mit Flüssigkeit gefüllten Körpers. Dieser besteht aus einem Träger (1) und einer damit verbundenen, in einem Winkelbereich von mehr als 0° bis maximal 90° neigbaren Ebene (8) mit einer darin ausgebildeten Abrollbahn (9) für den flüssigen oder mit Flüssigkeit gefüllten Körper. An der Ebene (8) sind mehrere Sensoren (11, 12) zur Erfassung der Rolldauer des Körpers entlang der Rollstrecke angeordnet. Erfindungsgemäß ist vorgesehen, dass die Einstellung des Neigungswinkels der Ebene (8) über ein Winkelmessgerät (10) erfolgt, wodurch ein Abrollwinkel erfassbar ist, bei dem der Körper in Bewegung gerät. Aus der Rolldauer, der Rollstrecke und dem Abrollwinkel wird der Kontaktwinkel des Körpers ermittelt. Y1 - 2014 PB - Deutsches Patent- und Markenamt CY - München ER - TY - PAT A1 - Al-Kaidy, Huschyar A1 - Tippkötter, Nils A1 - Ulber, Roland T1 - A system and a method for the implementation of chemical, biological or physical reactions [Europäische Patentanmeldung] N2 - The invention relates to a system for the implementation of chemical, biological or physical reactions, consisting of - one or more magnetic micro-reactors, each comprising a shell made of hydrophobic magnetic nanoparticles encapsulating an aqueous core, - a plane platform comprising a surface to receive the micro-reactors, - a source that generates a magnetic field above or underneath the platform for manipulating the one or more hydrophobic magnetic micro-reactors, or for moving them along the surface of the platform from one position to another position, characterized in that the aqueous core of the one or more magnetic micro-reactors contains a reaction solution or buffer, and wherein the magnetic field generated by the source correlates to a defined position on the surface of the platform. Y1 - 2013 PB - Europäisches Patentamt CY - Den Hague ER - TY - PAT A1 - Stadtmüller, Ralf A1 - Tippkötter, Nils A1 - Ulber, Roland T1 - A method for production of single-stranded nucleic acids [Europäische Patentanmeldung] Y1 - 2013 PB - Europäisches Patentamt CY - Den Hague ER - TY - JOUR A1 - Tippkötter, Nils A1 - Roikaew, N. A1 - Ulber, R. T1 - Nitratentfernung aus Molkekonzentrat mit biotechnologischer Regeneration der Abwässer JF - Deutsche Milchwirtschaft Y1 - 2007 SN - 0012-0480 VL - 58 IS - 15 SP - 540 EP - 542 ER - TY - JOUR A1 - Tippkötter, Nils A1 - Roikaew, W. A1 - Ulber, R. T1 - Nitrate removal from whey concentrate with biotechnological regeneration of the waste water JF - European dairy magazine : EDM Y1 - 2008 SN - 0936-6318 IS - 1 SP - 30 EP - 32 ER - TY - JOUR A1 - Ulber, R. A1 - Tippkötter, Nils A1 - Buchholz, H. A1 - Demmer, W. A1 - Scheper, T. T1 - Innovative Verfahren in der Molkeaufarbeitung zur Gewinnung neuer Produkte JF - Deutsche Milchwirtschaft Y1 - 2008 SN - 0012-0480 VL - 59 IS - 19 SP - 704 EP - 706 ER - TY - JOUR A1 - Braband, Henrik A1 - Paulßen, Elisabeth A1 - Abram, Ulrich T1 - Nitridorhenium(V) Complexes with 1,3-Dialkyl-4,5-dimethylimidazole-2-ylidenes JF - Zeitschrift für anorganische und allgemeine Chemie : ZAAC = Journal of inorganic and general chemistry Y1 - 2006 U6 - http://dx.doi.org/10.1002/zaac.200600002 SN - 1521-3749 VL - 632 IS - 6 SP - 1051 EP - 1056 ER - TY - JOUR A1 - Paulßen, Elisabeth A1 - Kückmann, Theresa A1 - Abram, Ulrich T1 - Silver(I) Complexes of 1,3-Dialkyl-4,5-dimethylimidazol-2-ylidenes and their Use as Precursors for the Synthesis of Rhenium(V) NHC Complexes JF - Zeitschrift für anorganische und allgemeine Chemie : ZAAC = Journal of inorganic and general chemistry Y1 - 2007 U6 - http://dx.doi.org/10.1002/zaac.200700021 SN - 1521-3749 VL - 633 IS - 5-6 SP - 830 EP - 834 ER - TY - JOUR A1 - Braband, Henrik A1 - Yegen, Eda A1 - Paulßen, Elisabeth A1 - Abram, Ulrich T1 - [{ReN(PMe2Ph)3}{ReO3N}]2 – Structural Evidence for the Nitridotrioxorhenate(VII) Anion, [ReO3N]2− JF - Zeitschrift für anorganische und allgemeine Chemie : ZAAC = Journal of inorganic and general chemistry Y1 - 2005 U6 - http://dx.doi.org/10.1002/zaac.200500240 SN - 1521-3749 VL - 631 IS - 12 SP - 2408 EP - 2410 ER - TY - JOUR A1 - Paulßen, Elisabeth A1 - Kong, Shushu A1 - Arciszewski, Pawel A1 - Wielbalck, Swantje A1 - Abram, Ulrich T1 - Aryl and NHC Compounds of Technetium and Rhenium JF - Journal of the American Chemical Society N2 - Air- and water-stable phenyl complexes with nitridotechnetium(V) cores can be prepared by straightforward procedures. [TcNPh2(PPh3)2] is formed by the reaction of [TcNCl2(PPh3)2] with PhLi. The analogous N-heterocyclic carbene (NHC) compound [TcNPh2(HLPh)2], where HLPh is 1,3,4-triphenyl-1,2,4-triazol-5-ylidene, is available from (NBu4)[TcNCl4] and HLPh or its methoxo-protected form. The latter compound allows the comparison of different Tc–C bonds within one compound. Surprisingly, the Tc chemistry with such NHCs does not resemble that of corresponding Re complexes, where CH activation and orthometalation dominate. Y1 - 2012 U6 - http://dx.doi.org/10.1021/ja3033718 SN - 1520-5126 VL - 134 IS - 22 SP - 9118 EP - 9121 PB - ACS Publications CY - Washington, DC ER - TY - JOUR A1 - Paulßen, Elisabeth A1 - Ngyugen, Hung Huy A1 - Kahlcke, Nils A1 - Deflon, Victor M. A1 - Abram, Ulrich T1 - Tricarbonyltechnetium(I) and -rhenium(I) complexes with N′-thiocarbamoylpicolylbenzamidines JF - Polyhedron N2 - N,N-Dialkylamino(thiocarbonyl)-N′-picolylbenzamidines react with (NEt4)2[M(CO)3X3] (M = Re, X = Br; M = Tc, X = Cl) under formation of neutral [M(CO)3L] complexes in high yields. The monoanionic NNS ligands bind in a facial coordination mode and can readily be modified at the (CS)NR1R2 moiety. The complexes [99Tc(CO)3(LPyMor)] and [Re(CO)3(L)] (L = LPyMor, LPyEt) were characterized by X-ray diffraction. Reactions of [99mTc(CO)3(H2O)3]+ with the N′-thiocarbamoylpicolylbenzamidines give the corresponding 99mTc complexes. The ester group in HLPyCOOEt allows linkage between biomolecules and the metal core. Y1 - 2012 U6 - http://dx.doi.org/10.1016/j.poly.2012.04.008 SN - 0277-5387 VL - 40 IS - 1 SP - 153 EP - 158 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Pellegrini, Paul A. A1 - Howell, Nicholas R. A1 - Shepherd, Rachael K. A1 - Lengkeek, Nigel A. A1 - Paulßen, Elisabeth A1 - Katsifis, Andrew G. A1 - Greguric, Ivan T1 - Synthesis and Radiolabelling of DOTA-Linked Glutamine Analogues with 67,68Ga as Markers for Increased Glutamine Metabolism in Tumour Cells JF - Molecules Y1 - 2013 U6 - http://dx.doi.org/10.3390/molecules18067160 SN - 1420-3049 VL - 18 IS - 6 SP - 7160 EP - 7178 PB - MDPI CY - Basel ER - TY - JOUR A1 - Paulßen, Elisabeth A1 - Le, Van So A1 - Lengkeek, Nigel A1 - Pellegrini, Paul A1 - Jackson, Tim A1 - Greguric, Ivan A1 - Weiner, Ron T1 - Influence of Metal Ions on the 68Ga-labeling of DOTATATE JF - Applied Radiation and Isotopes Y1 - 2013 U6 - http://dx.doi.org/10.1016/j.apradiso.2013.08.010 SN - 1872-9800 VL - 82 SP - 232 EP - 238 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Paulßen, Elisabeth A1 - Lengkeek, Nigel A. A1 - Le, Van So A1 - Pellegrini, Paul A. A1 - Greguric, Ivan A1 - Weiner, Ron T1 - The role of additives in moderating the influence of Fe(III) and Cu(II) on the radiochemical yield of [⁶⁸Ga(DOTATATE)] JF - Applied Radiation and Isotopes N2 - [⁶⁸Ga(DOTATATE)] has demonstrated its clinical usefulness. Both Fe³⁺ and Cu²⁺, potential contaminants in Gallium-68 generator eluent, substantially reduce the radiochemical (RC) yield of [⁶⁸Ga(DOTATATE)] if the metal/ligand ratio of 1:1 is exceeded. A variety of compounds were examined for their potential ability to reduce this effect. Most had no effect on RC yield. However, addition of phosphate diminished the influence of Fe³⁺ by likely forming an insoluble iron salt. Addition of ascorbic acid reduced Cu²⁺ and Fe³⁺ to Cu⁺ and Fe²⁺ respectively, both of which have limited impact on RC yields. At low ligand amounts (5 nmol DOTATATE), the addition of 30 nmol phosphate (0.19 mM) increased the tolerance of Fe3⁺ from 4 nmol to 10 nmol (0.06 mM), while the addition of ascorbic acid allowed high RC yields (>95%) in the presence of 40 nmol Fe³⁺ (0.25 mM) and 100 nmol Cu²⁺ (0.63 mM). The effect of ascorbic acid was highly pH-dependant, and gave optimal results at pH 3. Y1 - 2016 U6 - http://dx.doi.org/10.1016/j.apradiso.2015.09.008 SN - 1872-9800 VL - 107 SP - 13 EP - 16 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Paulßen, Elisabeth A1 - Hoehr, Cornelia A1 - Hou, Xinchi A1 - Hanemaayer, Victoire A1 - Zeisler, Stefan A1 - Adam, Michael J. A1 - Ruth, Thomas J. A1 - Celler, Anna A1 - Buckley, Ken A1 - Benard, Francois A1 - Schaffer, Paul T1 - Production of Y-86 and other radiometals for research purposes using a solution target system JF - Nuclear medicine and biology Y1 - 2015 U6 - http://dx.doi.org/10.1016/j.nucmedbio.2015.06.005 SN - 1872-9614 VL - 42 IS - 11 SP - 842 EP - 849 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Hoehr, Cornelia A1 - Paulßen, Elisabeth A1 - Benard, Francois A1 - Lee, Chris Jaeil A1 - Hou, Xinchi A1 - Badesso, Brian A1 - Ferguson, Simon A1 - Miao, Qing A1 - Yang, Hua A1 - Buckley, Ken A1 - Hanemaayer, Victoire A1 - Zeisler, Stefan A1 - Ruth, Thomas A1 - Celler, Anna A1 - Schaffer, Paul T1 - ⁴⁴ᶢSc production using a water target on a 13 MeV cyclotron JF - Nuclear medicine and biology N2 - Access to promising radiometals as isotopes for novel molecular imaging agents requires that they are routinely available and inexpensive to obtain. Proximity to a cyclotron center outfitted with solid target hardware, or to an isotope generator for the metal of interest is necessary, both of which can introduce significant hurdles in development of less common isotopes. Herein, we describe the production of ⁴⁴Sc (t₁⸝₂ = 3.97 h, Eavg,β⁺ = 1.47 MeV, branching ratio = 94.27%) in a solution target and an automated loading system which allows a quick turn-around between different radiometallic isotopes and therefore greatly improves their availability for tracer development. Experimental yields are compared to theoretical calculations. Y1 - 2014 U6 - http://dx.doi.org/10.1016/j.nucmedbio.2013.12.016 SN - 1872-9614 VL - 41 IS - 5 SP - 401 EP - 406 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Infantino, Angelo A1 - Paulßen, Elisabeth A1 - Mostacci, Domiziano A1 - Schaffer, Paul A1 - Trinczek, Michael A1 - Hoehr, Cornelia T1 - Assessment of the production of medical isotopes using the Monte Carlo code FLUKA: Simulations against experimental measurements JF - Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms N2 - The Monte Carlo code FLUKA is used to simulate the production of a number of positron emitting radionuclides, ¹⁸F, ¹³N, ⁹⁴Tc, ⁴⁴Sc, ⁶⁸Ga, ⁸⁶Y, ⁸⁹Zr, ⁵²Mn, ⁶¹Cu and ⁵⁵Co, on a small medical cyclotron with a proton beam energy of 13 MeV. Experimental data collected at the TR13 cyclotron at TRIUMF agree within a factor of 0.6 ± 0.4 with the directly simulated data, except for the production of ⁵⁵Co, where the simulation underestimates the experiment by a factor of 3.4 ± 0.4. The experimental data also agree within a factor of 0.8 ± 0.6 with the convolution of simulated proton fluence and cross sections from literature. Overall, this confirms the applicability of FLUKA to simulate radionuclide production at 13 MeV proton beam energy. Y1 - 2016 U6 - http://dx.doi.org/10.1016/j.nimb.2015.10.067 SN - 1872-9584 VL - 366 SP - 117 EP - 123 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Liu, Z. A1 - Schaap, K. S. A1 - Ballemans, L. A1 - de Blois, E. A1 - Rohde, M. A1 - Paulßen, Elisabeth T1 - Measurement of reaction kinetics of [177Lu]Lu-DOTA-TATE using a microfluidic system JF - Dalton Transactions Y1 - 2017 U6 - http://dx.doi.org/10.1039/C7DT01830D SN - 1477-9234 VL - 46 IS - 42 SP - 14669 EP - 14676 ER - TY - CHAP A1 - Al-Kaidy, H. A1 - Ulber, R. A1 - Tippkötter, Nils T1 - A platform technology for the automated reaction control in magnetizable micro-fluidic droplets T2 - Biomaterials - made in bioreactors : book of abstracts, May 26 - 28, 2014, Radisson Blu Park Hotel and Conference Dentre, Radebeul, Germany Y1 - 2014 SP - 21 EP - 22 PB - DECHEMA CY - Frankfurt am Main ER - TY - CHAP A1 - Tippkötter, Nils A1 - Roikaew, W. A1 - Ulber, R. T1 - An automated pilot plant for the bioengineering processing of concentrated whey T2 - European BioPerspectives : in cooperation with BIOTECHNICA 2008 : 7 - 9 October 2008 Hannover, Germany ; book of abstracts ; abstracts, poster programme Y1 - 2008 SP - 98 PB - Dechema CY - Frankfurt am Main ER - TY - CHAP A1 - Tippkötter, Nils A1 - Möhring, S. A1 - Maurer, S. A1 - Roth, J. T1 - Dezentrale Vorbehandlung und Verarbeitung pflanzlicher Reststoffe für Bioraffinerien T2 - Kurzfassungen der Vorträge nach Sessions : Frühjahrstagung der Biotechnologen 2013, 4. - 5. März 2013, Dechema-Haus, Frankfurt am Main Y1 - 2013 SP - 5 CY - Frankfurt am Main ER - TY - CHAP A1 - Tippkötter, Nils T1 - Biotechnologische Gewinnung von Wertstoffen aus Molke : BiobasedWorld - Innovation in food T2 - Biotechnica 2013 : European biotechnology science & industry news Y1 - 2013 VL - 12 IS - 9, special SP - 33 EP - 50 ER - TY - CHAP A1 - Tippkötter, Nils A1 - Stückmann, H. A1 - Winkelmann, G. A1 - Noack, U. A1 - Beutel, S. A1 - Scheper, T. A1 - Ulber, R. T1 - Optimisation of antibody-labelling of gold colloids for their application in an immunchromatographic assay for microcystin-LR T2 - European BioPerspectives : celebrating the 25th DECHEMA annual convention of biotechnologists ; 30 May - 1 June 2007, Cologne, Germany ; book of abstracts ; abstracts, poster programme Y1 - 2007 SP - 126 PB - Dechema CY - Frankfurt am Main ER - TY - JOUR A1 - Druckenmüller, Katharina A1 - Günther, Klaus A1 - Elbers, Gereon T1 - Near-infrared spectroscopy (NIRS) as a tool to monitor exhaust air from poultry operations JF - Science of the Total Environment N2 - Intensive poultry operation systems emit a considerable volume of inorganic and organic matter in the surrounding environment. Monitoring cleaning properties of exhaust air cleaning systems and to detect small but significant changes in emission characteristics during a fattening cycle is important for both emission and fattening process control. In the present study, we evaluated the potential of near-infrared spectroscopy (NIRS) combined with chemometric techniques as a monitoring tool of exhaust air from poultry operation systems. To generate a high-quality data set for evaluation, the exhaust air of two poultry houses was sampled by applying state-of-the-art filter sampling protocols. The two stables were identical except for one crucial difference, the presence or absence of an exhaust air cleaning system. In total, twenty-one exhaust air samples were collected at the two sites to monitor spectral differences caused by the cleaning device, and to follow changes in exhaust air characteristics during a fattening period. The total dust load was analyzed by gravimetric determination and included as a response variable in multivariate data analysis. The filter samples were directly measured with NIR spectroscopy. Principal component analysis (PCA), linear discriminant analysis (LDA), and factor analysis (FA) were effective in classifying the NIR exhaust air spectra according to fattening day and origin. The results indicate that the dust load and the composition of exhaust air (inorganic or organic matter) substantially influence the NIR spectral patterns. In conclusion, NIR spectroscopy as a tool is a promising and very rapid way to detect differences between exhaust air samples based on still not clearly defined circumstances triggered during a fattening period and the availability of an exhaust air cleaning system. Y1 - 2018 U6 - http://dx.doi.org/10.1016/j.scitotenv.2018.02.072 SN - 0048-9697 VL - 630 SP - 536 EP - 543 PB - Elsevier CY - Amsterdam ER - TY - THES A1 - Temiz Artmann, Aysegül T1 - Sicanlarda egzersiz sonrasi oksidatif hasar ve eritrosit membran degisikliklerinin hemoreolojik etkileri T1 - The effect of oxidative stress and erythrocyte membrane changes on hemorheological factors following exercise in rats Y1 - 2001 N1 - Dokuz Eylül Üniversitesi, Izmir, Diss., 2001. Veröffentlicht unter Temiz, Aysegül ER - TY - BOOK A1 - Artmann, Gerhard A1 - Temiz Artmann, Aysegül A1 - Zhubanova, Azhar A. A1 - Digel, Ilya ED - Artmann, Gerhard ED - Temiz Artmann, Aysegül ED - Zhubanova, Azhar A. ED - Digel, Ilya T1 - Biological, physical and technical basics of cell engineering Y1 - 2018 SN - 978-981-10-7903-0 PB - Springer CY - Singapore ER - TY - JOUR A1 - Molinnus, Denise A1 - Muschallik, Lukas A1 - Gonzalez, Laura Osorio A1 - Bongaerts, Johannes A1 - Wagner, Torsten A1 - Selmer, Thorsten A1 - Siegert, Petra A1 - Keusgen, Michael A1 - Schöning, Michael Josef T1 - Development and characterization of a field-effect biosensor for the detection of acetoin JF - Biosensors and Bioelectronics N2 - A capacitive electrolyte-insulator-semiconductor (EIS) field-effect biosensor for acetoin detection has been presented for the first time. The EIS sensor consists of a layer structure of Al/p-Si/SiO₂/Ta₂O₅/enzyme acetoin reductase. The enzyme, also referred to as butane-2,3-diol dehydrogenase from B. clausii DSM 8716T, has been recently characterized. The enzyme catalyzes the (R)-specific reduction of racemic acetoin to (R,R)- and meso-butane-2,3-diol, respectively. Two different enzyme immobilization strategies (cross-linking by using glutaraldehyde and adsorption) have been studied. Typical biosensor parameters such as optimal pH working range, sensitivity, hysteresis, linear concentration range and long-term stability have been examined by means of constant-capacitance (ConCap) mode measurements. Furthermore, preliminary experiments have been successfully carried out for the detection of acetoin in diluted white wine samples. Y1 - 2018 U6 - http://dx.doi.org/10.1016/j.bios.2018.05.023 VL - 115 SP - 1 EP - 6 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Pilas, Johanna A1 - Yazici, Y. A1 - Selmer, Thorsten A1 - Keusgen, M. A1 - Schöning, Michael Josef T1 - Application of a portable multi-analyte biosensor for organic acid determination in silage JF - Sensors N2 - Multi-analyte biosensors may offer the opportunity to perform cost-effective and rapid analysis with reduced sample volume, as compared to electrochemical biosensing of each analyte individually. This work describes the development of an enzyme-based biosensor system for multi-parametric determination of four different organic acids. The biosensor array comprises five working electrodes for simultaneous sensing of ethanol, formate, d-lactate, and l-lactate, and an integrated counter electrode. Storage stability of the biosensor was evaluated under different conditions (stored at +4 °C in buffer solution and dry at −21 °C, +4 °C, and room temperature) over a period of 140 days. After repeated and regular application, the individual sensing electrodes exhibited the best stability when stored at −21 °C. Furthermore, measurements in silage samples (maize and sugarcane silage) were conducted with the portable biosensor system. Comparison with a conventional photometric technique demonstrated successful employment for rapid monitoring of complex media. Y1 - 2018 U6 - http://dx.doi.org/10.3390/s18051470 SN - 1424-8220 VL - 18 IS - 5 PB - MDPI CY - Basel ER - TY - CHAP A1 - Artmann, Gerhard A1 - Meruvu, Haritha A1 - Kizildag, Sefa A1 - Temiz Artmann, Aysegül ED - Artmann, Gerhard ED - Temiz Artmann, Aysegül ED - Zhubanova, Azhar A. ED - Digel, Ilya T1 - Functional Toxicology and Pharmacology Test of Cell Induced Mechanical Tensile Stress in 2D and 3D Tissue Cultures T2 - Biological, Physical and Technical Basics of Cell Engineering N2 - Mechanical forces/tensile stresses are critical determinants of cellular growth, differentiation and migration patterns in health and disease. The innovative “CellDrum technology” was designed for measuring mechanical tensile stress of cultured cell monolayers/thin tissue constructs routinely. These are cultivated on very thin silicone membranes in the so-called CellDrum. The cell layers adhere firmly to the membrane and thus transmit the cell forces generated. A CellDrum consists of a cylinder which is sealed from below with a 4 μm thick, biocompatible, functionalized silicone membrane. The weight of cell culture medium bulbs the membrane out downwards. Membrane indentation is measured. When cells contract due to drug action, membrane, cells and medium are lifted upwards. The induced indentation changes allow for lateral drug induced mechanical tension quantification of the micro-tissues. With hiPS-induced (human) Cardiomyocytes (CM) the CellDrum opens new perspectives of individualized cardiac drug testing. Here, monolayers of self-beating hiPS-CMs were grown in CellDrums. Rhythmic contractions of the hiPS-cells induce membrane up-and-down deflections. The recorded cycles allow for single beat amplitude, single beat duration, integration of the single beat amplitude over the beat time and frequency analysis. Dose effects of agonists and antagonists acting on Ca2+ channels were sensitively and highly reproducibly observed. Data were consistent with published reference data as far as they were available. The combination of the CellDrum technology with hiPS-Cardiomyocytes offers a fast, facile and precise system for pharmacological and toxicological studies. It allows new preclinical basic as well as applied research in pharmacolgy and toxicology. Y1 - 2018 SN - 978-981-10-7904-7 U6 - http://dx.doi.org/10.1007/978-981-10-7904-7_7 SP - 157 EP - 192 PB - Springer CY - Singapore ER - TY - CHAP A1 - Duong, Minh Tuan A1 - Seifarth, Volker A1 - Temiz Artmann, Aysegül A1 - Artmann, Gerhard A1 - Staat, Manfred ED - Artmann, Gerhard ED - Temiz Artmann, Aysegül ED - Zhubanova, Azhar A. ED - Digel, Ilya T1 - Growth Modelling Promoting Mechanical Stimulation of Smooth Muscle Cells of Porcine Tubular Organs in a Fibrin-PVDF Scaffold T2 - Biological, Physical and Technical Basics of Cell Engineering N2 - Reconstructive surgery and tissue replacements like ureters or bladders reconstruction have been recently studied, taking into account growth and remodelling of cells since living cells are capable of growing, adapting, remodelling or degrading and restoring in order to deform and respond to stimuli. Hence, shapes of ureters or bladders and their microstructure change during growth and these changes strongly depend on external stimuli such as training. We present the mechanical stimulation of smooth muscle cells in a tubular fibrin-PVDFA scaffold and the modelling of the growth of tissue by stimuli. To this end, mechanotransduction was performed with a kyphoplasty balloon catheter that was guided through the lumen of the tubular structure. The bursting pressure was examined to compare the stability of the incubated tissue constructs. The results showed the significant changes on tissues with training by increasing the burst pressure as a characteristic mechanical property and the smooth muscle cells were more oriented with uniformly higher density. Besides, the computational growth models also exhibited the accurate tendencies of growth of the cells under different external stimuli. Such models may lead to design standards for the better layered tissue structure in reconstructing of tubular organs characterized as composite materials such as intestines, ureters and arteries. KW - Mechanical simulation KW - Growth modelling KW - Ureter KW - Bladder KW - Reconstruction Y1 - 2018 SN - 978-981-10-7904-7 U6 - http://dx.doi.org/10.1007/978-981-10-7904-7_9 SP - 209 EP - 232 PB - Springer CY - Singapore ER - TY - CHAP A1 - Wagemann, Kurt A1 - Tippkötter, Nils T1 - Biorefineries: a short introduction T2 - Biorefineries N2 - The terms bioeconomy and biorefineries are used for a variety of processes and developments. This short introduction is intended to provide a delimitation and clarification of the terminology as well as a classification of current biorefinery concepts. The basic process diagrams of the most important biorefinery types are shown. KW - Bioeconomy KW - Biorefinery definitions KW - Introduction KW - Process schemes KW - Renewable resources Y1 - 2019 SN - 978-3-319-97117-9 SN - 978-3-319-97119-3 U6 - http://dx.doi.org/10.1007/10_2017_4 N1 - (Advances in Biochemical Engineering/Biotechnology book series ; Vol. 166) SP - 1 EP - 11 PB - Springer CY - Cham ER - TY - JOUR A1 - Aboulnaga, E. A. A1 - Zou, H. A1 - Selmer, Thorsten A1 - Xian, M. T1 - Development of a plasmid-based, tunable, tolC-derived expression system for application in Cupriavidus necator H16 JF - Journal of Biotechnology N2 - Cupriavidus necator H16 gains increasing attention in microbial research and biotechnological application due to its diverse metabolic features. Here we present a tightly controlled gene expression system for C. necator including the pBBR1-vector that contains hybrid promoters originating from C. necator native tolC-promoter in combination with a synthetic tetO-operator. The expression of the reporter gene from these plasmids relies on the addition of the exogenous inducer doxycycline (dc). The novel expression system offers a combination of advantageous features as; (i) high and dose-dependent recombinant protein production, (ii) tight control with a high dynamic range (On/Off ratio), which makes it applicable for harmful pathways or for toxic protein production, (iii) comparable cheap inducer (doxycycline, dc), (iv) effective at low inducer concentration, that makes it useful for large scale application, (v) rapid, diffusion controlled induction, and (vi) the inducer does not interfere within the cell metabolism. As applications of the expression system in C. necator H16, the growth ability on glycerol was enhanced by constitutively expressing the E. coli glpk gene-encoding for glycerol kinase. Likewise, we used the system to overcome the expression toxicity of mevalonate pathway in C. necator H16. With this system, the mevalonate-genes were successfully introduced in the host and the recombinant strains could produce about 200 mg/l mevalonate. Y1 - 2018 U6 - http://dx.doi.org/10.1016/j.jbiotec.2018.03.007 SN - 0168-1656 VL - 274 SP - 15 EP - 27 PB - Elsevier CY - Amsterdam ER - TY - THES A1 - Temiz Artmann, Aysegül T1 - Sicanlarda, akut egzersiz sonucu gelisen oksidan stresin lökosit aktivasyon degisiklikleri ile ile olan iliskisi ve eritrosit deformabilitesine etkisi T1 - The oxidative stress effect on leukocyte activation and erythrocyte deformability after acute exercise in rats Y1 - 1996 N1 - Dokuz Eylül Üniversitesi, Izmir, Diss., 1996. Veröffentlicht unter Temiz, Ayşegül ER - TY - CHAP A1 - Ulber, Roland A1 - Muffler, Kai A1 - Tippkötter, Nils A1 - Hirth, Thomas A1 - Sell, Dieter ED - Ulber, Roland ED - Sell, Dieter ED - Hirth, Thomas T1 - Introduction to Renewable Resources in the Chemical Industry T2 - Renewable raw materials : new feedstocks for the chemical industry Y1 - 2011 SN - 978-3-527-32548-1 SP - 1 EP - 6 PB - Wiley-VCH-Verlag CY - Weinheim ET - 1. Auflage ER - TY - JOUR A1 - Teumer, T. A1 - Capitain, C. A1 - Ross-Jones, J. A1 - Tippkötter, Nils A1 - Rädle, M. A1 - Methner, F.-J. T1 - In-line Haze Monitoring Using a Spectrally Resolved Back Scattering Sensor JF - BrewingScience N2 - In the present work an optical sensor in combination with a spectrally resolved detection device for in-line particle-size-monitoring for quality control in beer production is presented. The principle relies on the size and wavelength dependent backscatter of growing particles in fluids. Measured interference structures of backscattered light are compared with calculated theoretical values, based on Mie-Theory, and fitted with a linear least square method to obtain particle size distributions. For this purpose, a broadband light source in combination with a process-CCD-spectrometer (charge ? coupled device spectrometer) and process adapted fiber optics are used. The goal is the development of an easy and flexible measurement device for in-line-monitoring of particle size. The presented device can be directly installed in product fill tubes or vessels, follows CIP- (cleaning in place) and removes the need of sample taking. A proof of concept and preliminary results, measuring protein precipitation, are presented. Y1 - 2018 SN - 1613-2041 VL - 71 IS - 5/6 SP - 49 EP - 55 PB - Fachverlag Hans Carl CY - Nürnberg ER - TY - CHAP A1 - Tippkötter, Nils A1 - Möhring, Sophie A1 - Roth, Jasmine A1 - Wulfhorst, Helene T1 - Logistics of lignocellulosic feedstocks: preprocessing as a preferable option T2 - Biorefineries N2 - In comparison to crude oil, biorefinery raw materials are challenging in concerns of transport and storage. The plant raw materials are more voluminous, so that shredding and compacting usually are necessary before transport. These mechanical processes can have a negative influence on the subsequent biotechnological processing and shelf life of the raw materials. Various approaches and their effects on renewable raw materials are shown. In addition, aspects of decentralized pretreatment steps are discussed. Another important aspect of pretreatment is the varying composition of the raw materials depending on the growth conditions. This problem can be solved with advanced on-site spectrometric analysis of the material. KW - Analytics KW - Decentral KW - Mechanical KW - On-site KW - Pre-treatment Y1 - 2019 SN - 978-3-319-97117-9 SN - 978-3-319-97119-3 U6 - http://dx.doi.org/10.1007/10_2017_58 N1 - Advances in biochemical engineering/biotechnology ; Vol. 166 SP - 43 EP - 68 PB - Springer CY - Cham ER - TY - CHAP A1 - Duwe, A. A1 - Tippkötter, Nils A1 - Ulber, R. T1 - Lignocellulose-Biorefinery: Ethanol-Focused T2 - Biorefineries N2 - The development prospects of the world markets for petroleum and other liquid fuels are diverse and partly contradictory. However, comprehensive changes for the energy supply of the future are essential. Notwithstanding the fact that there are still very large deposits of energy resources from a geological point of view, the finite nature of conventional oil reserves is indisputable. To reduce our dependence on oil, the EU, the USA, and other major economic zones rely on energy diversification. For this purpose, alternative materials and technologies are being sought, and is most obvious in the transport sector. The objective is to progressively replace fossil fuels with renewable and more sustainable fuels. In this respect, biofuels have a pre-eminent position in terms of their capability of blending with fossil fuels and being usable in existing cars without substantial modification. Ethanol can be considered as the primary renewable liquid fuel. In this chapter enzymes, micro-organisms, and processes for ethanol production based on renewable resources are described. KW - Bioethanol KW - Biorefinery KW - Lignocellulose feedstook Y1 - 2018 U6 - http://dx.doi.org/10.1007/10_2016_72 N1 - Part of the Advances in Biochemical Engineering/Biotechnology book series (ABE,volume 166) SP - 177 EP - 215 PB - Springer CY - Cham ER - TY - RPRT A1 - Tippkötter, Nils T1 - Lokale Vorbehandlung nachwachsender Rohstoffe für Bioraffinerien (BioSats) : Schlussbericht zum Vorhaben : Laufzeit: 01.03.2012 bis 30.04.2017 Y1 - 2018 U6 - http://dx.doi.org/10.2314/GBV:1024204243 ER - TY - JOUR A1 - Eckert, Alexander A1 - Rudolph, Tobias A1 - Guo, Jiaqi A1 - Mang, Thomas A1 - Walther, Andreas T1 - Exceptionally Ductile and Tough Biomimetic Artificial Nacre with Gas Barrier Function JF - Advanced Materials N2 - Synthetic mimics of natural high-performance structural materials have shown great and partly unforeseen opportunities for the design of multifunctional materials. For nacre-mimetic nanocomposites, it has remained extraordinarily challenging to make ductile materials with high stretchability at high fractions of reinforcements, which is however of crucial importance for flexible barrier materials. Here, highly ductile and tough nacre-mimetic nanocomposites are presented, by implementing weak, but many hydrogen bonds in a ternary nacre-mimetic system consisting of two polymers (poly(vinyl amine) and poly(vinyl alcohol)) and natural nanoclay (montmorillonite) to provide efficient energy dissipation and slippage at high nanoclay content (50 wt%). Tailored interactions enable exceptional combinations of ductility (close to 50% strain) and toughness (up to 27.5 MJ m⁻³). Extensive stress whitening, a clear sign of high internal dynamics at high internal cohesion, can be observed during mechanical deformation, and the materials can be folded like paper into origami planes without fracture. Overall, the new levels of ductility and toughness are unprecedented in highly reinforced bioinspired nanocomposites and are of critical importance to future applications, e.g., as barrier materials needed for encapsulation and as a printing substrate for flexible organic electronics. Y1 - 2018 U6 - http://dx.doi.org/10.1002/adma.201802477 VL - 30 IS - 32 SP - Article number 1802477 PB - Wiley-VCH ER - TY - JOUR A1 - Müller, Janina A1 - Beckers, Mario A1 - Mußmann, Nina A1 - Bongaerts, Johannes A1 - Büchs, Jochen T1 - Elucidation of auxotrophic deficiencies of Bacillus pumilus DSM 18097 to develop a defined minimal medium JF - Microbial Cell Factories N2 - Background Culture media containing complex compounds like yeast extract or peptone show numerous disadvantages. The chemical composition of the complex compounds is prone to significant variations from batch to batch and quality control is difficult. Therefore, the use of chemically defined media receives more and more attention in commercial fermentations. This concept results in better reproducibility, it simplifies downstream processing of secreted products and enable rapid scale-up. Culturing bacteria with unknown auxotrophies in chemically defined media is challenging and often not possible without an extensive trial-and-error approach. In this study, a respiration activity monitoring system for shake flasks and its recent version for microtiter plates were used to clarify unknown auxotrophic deficiencies in the model organism Bacillus pumilus DSM 18097. Results Bacillus pumilus DSM 18097 was unable to grow in a mineral medium without the addition of complex compounds. Therefore, a rich chemically defined minimal medium was tested containing basically all vitamins, amino acids and nucleobases, which are essential ingredients of complex components. The strain was successfully cultivated in this medium. By monitoring of the respiration activity, nutrients were supplemented to and omitted from the rich chemically defined medium in a rational way, thus enabling a systematic and fast determination of the auxotrophic deficiencies. Experiments have shown that the investigated strain requires amino acids, especially cysteine or histidine and the vitamin biotin for growth. Conclusions The introduced method allows an efficient and rapid identification of unknown auxotrophic deficiencies and can be used to develop a simple chemically defined tailor-made medium. B. pumilus DSM 18097 was chosen as a model organism to demonstrate the method. However, the method is generally suitable for a wide range of microorganisms. By combining a systematic combinatorial approach based on monitoring the respiration activity with cultivation in microtiter plates, high throughput experiments with high information content can be conducted. This approach facilitates media development, strain characterization and cultivation of fastidious microorganisms in chemically defined minimal media while simultaneously reducing the experimental effort. Y1 - 2018 U6 - http://dx.doi.org/10.1186/s12934-018-0956-1 SN - 1475-2859 VL - 17 IS - 1 SP - Article No. 106 PB - BioMed Central ER - TY - JOUR A1 - Röhlen, Desiree A1 - Pilas, Johanna A1 - Dahmen, Markus A1 - Keusgen, Michael A1 - Selmer, Thorsten A1 - Schöning, Michael Josef T1 - Toward a Hybrid Biosensor System for Analysis of Organic and Volatile Fatty Acids in Fermentation Processes JF - Frontiers in Chemistry N2 - Monitoring of organic acids (OA) and volatile fatty acids (VFA) is crucial for the control of anaerobic digestion. In case of unstable process conditions, an accumulation of these intermediates occurs. In the present work, two different enzyme-based biosensor arrays are combined and presented for facile electrochemical determination of several process-relevant analytes. Each biosensor utilizes a platinum sensor chip (14 × 14 mm²) with five individual working electrodes. The OA biosensor enables simultaneous measurement of ethanol, formate, d- and l-lactate, based on a bi-enzymatic detection principle. The second VFA biosensor provides an amperometric platform for quantification of acetate and propionate, mediated by oxidation of hydrogen peroxide. The cross-sensitivity of both biosensors toward potential interferents, typically present in fermentation samples, was investigated. The potential for practical application in complex media was successfully demonstrated in spiked sludge samples collected from three different biogas plants. Thereby, the results obtained by both of the biosensors were in good agreement to the applied reference measurements by photometry and gas chromatography, respectively. The proposed hybrid biosensor system was also used for long-term monitoring of a lab-scale biogas reactor (0.01 m³) for a period of 2 months. In combination with typically monitored parameters, such as gas quality, pH and FOS/TAC (volatile organic acids/total anorganic carbonate), the amperometric measurements of OA and VFA concentration could enhance the understanding of ongoing fermentation processes. Y1 - 2018 U6 - http://dx.doi.org/10.3389/fchem.2018.00284 IS - 6 PB - Frontiers CY - Lausanne ER - TY - JOUR A1 - Engel, Mareike A1 - Holtmann, Dirk A1 - Ulber, Roland A1 - Tippkötter, Nils T1 - Increased Biobutanol Production by Mediator‐Less Electro‐Fermentation JF - Biotechnology Journal N2 - A future bio-economy should not only be based on renewable raw materials but also in the raise of carbon yields of existing production routes. Microbial electrochemical technologies are gaining increased attention for this purpose. In this study, the electro-fermentative production of biobutanol with C. acetobutylicum without the use of exogenous mediators is investigated regarding the medium composition and the reactor design. It is shown that the use of an optimized synthetic culture medium allows higher product concentrations, increased biofilm formation, and higher conductivities compared to a synthetic medium supplemented with yeast extract. Moreover, the optimization of the reactor system results in a doubling of the maximum product concentrations for fermentation products. When a working electrode is polarized at −600 mV vs. Ag/AgCl, a shift from butyrate to acetone and butanol production is induced. This leads to an increased final solvent yield of Yᴀᴃᴇ = 0.202 gg⁻¹ (control 0.103 gg⁻¹), which is also reflected in a higher carbon efficiency of 37.6% compared to 23.3% (control) as well as a fourfold decrease in simplified E-factor to 0.43. The results are promising for further development of biobutanol production in bioelectrochemical systems in order to fulfil the principles of Green Chemistry. Y1 - 2018 U6 - http://dx.doi.org/10.1002/biot.201800514 SN - 1860-7314 IS - Volume 14, Issue 4 SP - Artikel 1800514 PB - Wiley-VCH ER - TY - CHAP A1 - Kazuki, Yasuhiro A1 - Kobayashi, Kaoru A1 - Hirabayashi, Masumi A1 - Abe, Satoshi A1 - Kajitani, Naoyo A1 - Kazuki, Kanoko A1 - Takehara, Shoko A1 - Takiguchi, Masato A1 - Satoh, Daisuke A1 - Kuze, Jiro A1 - Sakuma, Tetsushi A1 - Kaneko, Takehito A1 - Mashimo, Tomoji A1 - Osamura, Minori A1 - Hashimoto, Mari A1 - Wakatsuki, Riko A1 - Hirashima, Rika A1 - Fujiwara, Ryoichi A1 - Deguchi, Tsuneo A1 - Kurihara, Atsushi A1 - Tsukazaki, Yasuko A1 - Senda, Naoto A1 - Yamamoto, Takashi A1 - Scheer, Nico A1 - Oshimura, Mitsuo T1 - Humanized UGT2 and CYP3A transchromosomic rats for improved prediction of human drug metabolism T2 - PNAS Proceedings of the National Academy of Sciences of the United States of America Y1 - 2019 U6 - http://dx.doi.org/10.1073/pnas.1808255116 SN - 1091-6490 VL - 116 IS - 8 SP - 3072 EP - 3081 ER - TY - JOUR A1 - Wilson, C. E. A1 - Dickie, A. P. A1 - Schreiter, K. A1 - Wehr, R. A1 - Wilson, E. M. A1 - Bial, J. A1 - Scheer, Nico A1 - Wilson, I. D. A1 - Riley, R. J. T1 - The pharmacokinetics and metabolism of diclofenac in chimeric humanized and murinized FRG mice JF - Archives of Toxicology N2 - The pharmacokinetics of diclofenac were investigated following single oral doses of 10 mg/kg to chimeric liver humanized and murinized FRG and C57BL/6 mice. In addition, the metabolism and excretion were investigated in chimeric liver humanized and murinized FRG mice. Diclofenac reached maximum blood concentrations of 2.43 ± 0.9 µg/mL (n = 3) at 0.25 h post-dose with an AUCinf of 3.67 µg h/mL and an effective half-life of 0.86 h (n = 2). In the murinized animals, maximum blood concentrations were determined as 3.86 ± 2.31 µg/mL at 0.25 h post-dose with an AUCinf of 4.94 ± 2.93 µg h/mL and a half-life of 0.52 ± 0.03 h (n = 3). In C57BL/6J mice, mean peak blood concentrations of 2.31 ± 0.53 µg/mL were seen 0.25 h post-dose with a mean AUCinf of 2.10 ± 0.49 µg h/mL and a half-life of 0.51 ± 0.49 h (n = 3). Analysis of blood indicated only trace quantities of drug-related material in chimeric humanized and murinized FRG mice. Metabolic profiling of urine, bile and faecal extracts revealed a complex pattern of metabolites for both humanized and murinized animals with, in addition to unchanged parent drug, a variety of hydroxylated and conjugated metabolites detected. The profiles in humanized mice were different to those of both murinized and wild-type animals, e.g., a higher proportion of the dose was detected in the form of acyl glucuronide metabolites and much reduced amounts as taurine conjugates. Comparison of the metabolic profiles obtained from the present study with previously published data from C57BL/6J mice and humans revealed a greater, though not complete, match between chimeric humanized mice and humans, such that the liver humanized FRG model may represent a model for assessing the biotransformation of such compounds in humans. Y1 - 2018 U6 - http://dx.doi.org/10.1007/s00204-018-2212-1 SN - 1432-0738 VL - 92 IS - 6 SP - 1953 EP - 1967 PB - Springer ER - TY - JOUR A1 - Wilson, Ian D. A1 - Wilson, Claire E. A1 - Scheer, Nico A1 - Dickie, A.P. A1 - Schreiter, K. A1 - Wilson, E. M. A1 - Riley, R. J. A1 - Wehr, R. A1 - Bial, J. T1 - The Pharmacokinetics and Metabolism of Lumiracoxib in Chimeric Humanized and Murinized FRG Mice JF - Biochemical pharmacology Y1 - 2017 U6 - http://dx.doi.org/10.1016/j.bcp.2017.03.015 SN - 1873-2968 VL - Volume 135 SP - 139 EP - 150 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Zhang, Jin A1 - Heimbach, Tycho A1 - Scheer, Nico A1 - Barve, Avantika A1 - Li, Wenkui A1 - Lin, Wen A1 - He, Handan T1 - Clinical Exposure Boost Predictions by Integrating Cytochrome P450 3A4–Humanized Mouse Studies With PBPK Modeling JF - Journal of Pharmaceutical Sciences N2 - NVS123 is a poorly water-soluble protease 56 inhibitor in clinical development. Data from in vitro hepatocyte studies suggested that NVS123 is mainly metabolized by CYP3A4. As a consequence of limited solubility, NVS123 therapeutic plasma exposures could not be achieved even with high doses and optimized formulations. One approach to overcome NVS123 developability issues was to increase plasma exposure by coadministrating it with an inhibitor of CYP3A4 such as ritonavir. A clinical boost effect was predicted by using physiologically based pharmacokinetic (PBPK) modeling. However, initial boost predictions lacked sufficient confidence because a key parameter, fraction of drug metabolized by CYP3A4 (ƒₘCYP3A4), could not be estimated with accuracy on account of disconnects between in vitro and in vivo preclinical data. To accurately estimate ƒₘCYP3A4 in human, an in vivo boost effect study was conducted using CYP3A4-humanized mouse model which showed a 33- to 56-fold exposure boost effect. Using a top-down approach, human ƒₘCYP3A4 for NVS123 was estimated to be very high and included in the human PBPK modeling to support subsequent clinical study design. The combined use of the in vivo boost study in CYP3A4-humanized mouse model mice along with PBPK modeling accurately predicted the clinical outcome and identified a significant NVS123 exposure boost (∼42-fold increase) with ritonavir. Y1 - 2016 U6 - http://dx.doi.org/doi.org/10.1016/j.xphs.2016.01.021 SN - 0022-3549 VL - Volume 105 IS - Issue 4 SP - 1398 EP - 1404 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Dallas, Shannon A1 - Salphati, Laurent A1 - Gomez-Zepeda, David A1 - Wanek, Thomas A1 - Chen, Liangfu A1 - Chu, Xiaoyan A1 - Kunta, Jeevan A1 - Mezler, Mario A1 - Menet, Marie-Claude A1 - Chasseigneaux, Stephanie A1 - Declèves, Xavier A1 - Langer, Oliver A1 - Pierre, Esaie A1 - DiLoreto, Karen A1 - Hoft, Carolin A1 - Laplanche, Loic A1 - Pang, Jodie A1 - Pereira, Tony A1 - Andonian, Clara A1 - Simic, Damir A1 - Rode, Anja A1 - Yabut, Jocelyn A1 - Zhang, Xiaolin A1 - Scheer, Nico T1 - Generation and Characterization of a Breast Cancer Resistance Protein Humanized Mouse Model JF - Molecular Pharmacology N2 - Breast cancer resistance protein (BCRP) is expressed in various tissues, such as the gut, liver, kidney and blood brain barrier (BBB), where it mediates the unidirectional transport of substrates to the apical/luminal side of polarized cells. Thereby BCRP acts as an efflux pump, mediating the elimination or restricting the entry of endogenous compounds or xenobiotics into tissues and it plays important roles in drug disposition, efficacy and safety. Bcrp knockout mice (Bcrp−/−) have been used widely to study the role of this transporter in limiting intestinal absorption and brain penetration of substrate compounds. Here we describe the first generation and characterization of a mouse line humanized for BCRP (hBCRP), in which the mouse coding sequence from the start to stop codon was replaced with the corresponding human genomic region, such that the human transporter is expressed under control of the murine Bcrp promoter. We demonstrate robust human and loss of mouse BCRP/Bcrp mRNA and protein expression in the hBCRP mice and the absence of major compensatory changes in the expression of other genes involved in drug metabolism and disposition. Pharmacokinetic and brain distribution studies with several BCRP probe substrates confirmed the functional activity of the human transporter in these mice. Furthermore, we provide practical examples for the use of hBCRP mice to study drug-drug interactions (DDIs). The hBCRP mouse is a promising model to study the in vivo role of human BCRP in limiting absorption and BBB penetration of substrate compounds and to investigate clinically relevant DDIs involving BCRP. Y1 - 2016 U6 - http://dx.doi.org/10.1124/mol.115.102079 SN - 1521-0111 VL - 89 IS - 5 SP - 492 EP - 504 PB - ASPET CY - Bethesda, Md. ER - TY - JOUR A1 - Scheer, Nico A1 - Kapelyukh, Yury A1 - Rode, Anja A1 - Oswald, Stefan A1 - Busch, Diana A1 - Mclaughlin, Lesley A. A1 - Lin, De A1 - Henderson, Colin J. A1 - Wolf, C. Roland T1 - Defining Human Pathways of Drug Metabolism In Vivo through the Development of a Multiple Humanized Mouse Model JF - Drug Metabolism and Disposition Y1 - 2015 U6 - http://dx.doi.org/10.1124/dmd.115.065656 SN - 1521-009x VL - 43 IS - 11 SP - 1679 EP - 1690 PB - ASPET CY - Bethesda ER - TY - JOUR A1 - Hough, Lindsay B. A1 - Nalwalk, Julia W. A1 - Ding, Xinxin A1 - Scheer, Nico T1 - Opioid Analgesia in P450 Gene Cluster Knockout Mice: A Search for Analgesia-Relevant Isoforms JF - Drug Metabolism and Disposition Y1 - 2015 U6 - http://dx.doi.org/10.1124/dmd.115.065490 SN - 1521-009x VL - 43 IS - 9 SP - 1326 EP - 1330 ER - TY - JOUR A1 - Henderson, Colin J. A1 - Mclaughlin, Lesley A. A1 - Scheer, Nico A1 - Stanley, Lesley A. A1 - Wolf, C. Roland T1 - Cytochrome b5 Is a Major Determinant of Human Cytochrome P450 CYP2D6 and CYP3A4 Activity In Vivo s JF - Molecular Pharmacology Y1 - 2015 U6 - http://dx.doi.org/10.1124/mol.114.097394 SN - 1521-0111 VL - 87 IS - 4 SP - 733 EP - 739 PB - ASPET CY - Bethesda ER - TY - JOUR A1 - Luisier, Raphaëlle A1 - Lempiäinen, Harri A1 - Scherbichler, Nina A1 - Braeuning, Albert A1 - Geissler, Miriam A1 - Dubost, Valerie A1 - Müller, Arne A1 - Scheer, Nico A1 - Chibout, Salah-Dine A1 - Hara, Hisanori A1 - Picard, Frank A1 - Theil, Diethilde A1 - Couttet, Philippe A1 - Vitobello, Antonio A1 - Grenet, Olivier A1 - Grasl-Kraupp, Bettina A1 - Ellinger-Ziegelbauer, Heidrung A1 - Thomson, John P. A1 - Meehan, Richard R. A1 - Elcombe, Clifford R. A1 - Henderson, Colin J. A1 - Wolf, C. Roland A1 - Schwarz, Michael A1 - Moulin, Pierre A1 - Terranova, Remi A1 - Moggs, Jonathan G. T1 - Phenobarbital Induces Cell Cycle Transcriptional Responses in Mouse Liver Humanized for Constitutive Androstane and Pregnane X Receptors JF - Toxicological Sciences N2 - The constitutive androstane receptor (CAR) and the pregnane X receptor (PXR) are closely related nuclear receptors involved in drug metabolism and play important roles in the mechanism of phenobarbital (PB)-induced rodent nongenotoxic hepatocarcinogenesis. Here, we have used a humanized CAR/PXR mouse model to examine potential species differences in receptor-dependent mechanisms underlying liver tissue molecular responses to PB. Early and late transcriptomic responses to sustained PB exposure were investigated in liver tissue from double knock-out CAR and PXR (CARᴷᴼ-PXRᴷᴼ), double humanized CAR and PXR (CARʰ-PXRʰ), and wild-type C57BL/6 mice. Wild-type and CARʰ-PXRʰ mouse livers exhibited temporally and quantitatively similar transcriptional responses during 91 days of PB exposure including the sustained induction of the xenobiotic response gene Cyp2b10, the Wnt signaling inhibitor Wisp1, and noncoding RNA biomarkers from the Dlk1-Dio3 locus. Transient induction of DNA replication (Hells, Mcm6, and Esco2) and mitotic genes (Ccnb2, Cdc20, and Cdk1) and the proliferation-related nuclear antigen Mki67 were observed with peak expression occurring between 1 and 7 days PB exposure. All these transcriptional responses were absent in CARᴷᴼ-PXRᴷᴼ mouse livers and largely reversible in wild-type and CARʰ-PXRʰ mouse livers following 91 days of PB exposure and a subsequent 4-week recovery period. Furthermore, PB-mediated upregulation of the noncoding RNA Meg3, which has recently been associated with cellular pluripotency, exhibited a similar dose response and perivenous hepatocyte-specific localization in both wild-type and CARʰ-PXRʰ mice. Thus, mouse livers coexpressing human CAR and PXR support both the xenobiotic metabolizing and the proliferative transcriptional responses following exposure to PB. Y1 - 2014 U6 - http://dx.doi.org/https://doi.org/10.1093/toxsci/kfu038 SN - 1094-2025 VL - 139 IS - 2 SP - 501 EP - 511 PB - Oxford University Press CY - Oxford ER - TY - JOUR A1 - Salpati, Laurent A1 - Chu, Xiaoyan A1 - Chen, Liangfu A1 - Prasad, Bhagwat A1 - Dallas, Shannon A1 - Evers, Raymond A1 - Mamaril-Fishman, Donna A1 - Geier, Ethan G. A1 - Kehler, Jonathan A1 - Kunta, Jeevan A1 - Mezler, Mario A1 - Laplanche, Loic A1 - Pang, Jodie A1 - Soars, Matthew G. A1 - Unadkat, Jashvant D. A1 - van Waterschoot, Robert A.B. A1 - Yabut, Jocelyn A1 - Schinkel, Alfred H. A1 - Scheer, Nico A1 - Rode, Anja T1 - Evaluation of organic anion transporting polypeptide 1B1 and 1B3 humanized mice as a translational model to study the pharmacokinetics of statins JF - Drug Metabolism and Disposition N2 - Organic anion transporting polypeptide (Oatp) 1a/1b knockout and OATP1B1 and -1B3 humanized mouse models are promising tools for studying the roles of these transporters in drug disposition. Detailed characterization of these models will help to better understand their utility for predicting clinical outcomes. To advance this approach, we carried out a comprehensive analysis of these mouse lines by evaluating the compensatory changes in mRNA expression, quantifying the amounts of OATP1B1 and -1B3 protein by liquid chromatography–tandem mass spectrometry, and studying the active uptake in isolated hepatocytes and the pharmacokinetics of some prototypical substrates including statins. Major outcomes from these studies were 1) mostly moderate compensatory changes in only a few genes involved in drug metabolism and disposition, 2) a robust hepatic expression of OATP1B1 and -1B3 proteins in the respective humanized mouse models, and 3) functional activities of the human transporters in hepatocytes isolated from the humanized models with several substrates tested in vitro and with pravastatin in vivo. However, the expression of OATP1B1 and -1B3 in the humanized models did not significantly alter liver or plasma concentrations of rosuvastatin and pitavastatin compared with Oatp1a/1b knockout controls under the conditions used in our studies. Hence, although the humanized OATP1B1 and -1B3 mice showed in vitro and/or in vivo functional activity with some statins, further characterization of these models is required to define their potential use and limitations in the prediction of drug disposition and drug-drug interactions in humans. Y1 - 2014 U6 - http://dx.doi.org/10.1124/dmd.114.057976 SN - 1521-009X VL - 42 IS - 8 SP - 1301 EP - 1313 PB - ASPET CY - Bethesda, Md. ER - TY - BOOK A1 - Wagemann, Kurt A1 - Tippkötter, Nils T1 - Biorefineries / Kurt Wagemann, Nils Tippkötter (editors) T3 - Advances in biochemical engineering/biotechnology book series (ABE) Y1 - 2019 SN - 978-3-319-97117-9 SN - 978-3-319-97119-3 U6 - http://dx.doi.org/10.1007/978-3-319-97119-3 PB - Springer CY - Cham (Switzerland) ER - TY - JOUR A1 - Raupp, Sebastian M. A1 - Schmitt, Marcel A1 - Walz, Anna-Lena A1 - Diehm, Ralf A1 - Hummel, Helga A1 - Scharfer, Philip A1 - Schabel, Wilhelm T1 - Slot die stripe coating of low viscous fluids JF - Journal of Coatings Technology and Research N2 - Slot die coating is applied to deposit thin and homogenous films in roll-to-roll and sheet-to-sheet applications. The critical step in operation is to choose suitable process parameters within the process window. In this work, we investigate an upper limit for stripe coatings. This maximum film thickness is characterized by stripe merging which needs to be avoided in a stable process. It is shown that the upper limit reduces the process window for stripe coatings to a major extent. As a result, stripe coatings at large coating gaps and low viscosities are only possible for relatively thick films. Explaining the upper limit, a theory of balancing the side pressure in the gap region in the cross-web direction has been developed. Y1 - 2018 U6 - http://dx.doi.org/10.1007/s11998-017-0039-y SN - 1935-3804 VL - 15 IS - 5 SP - 899 EP - 911 PB - Springer ER - TY - JOUR A1 - Scheer, Nico A1 - Mclaughlin, Lesley A. A1 - Rode, Anja A1 - MacLeod, Alastair Kenneth A1 - Henderson, Colin J. A1 - Wolf, Roland C. T1 - Deletion of thirty murine cytochrome P450 genes results in viable mice with compromised drug metabolism JF - Drug Metabolism and Disposition N2 - In humans, 75% of all drugs are metabolized by the cytochrome P450-dependent monooxygenase system. Enzymes encoded by the CYP2C, CYP2D, and CYP3A gene clusters account for ∼80% of this activity. There are profound species differences in the multiplicity of cytochrome P450 enzymes, and the use of mouse models to predict pathways of drug metabolism is further complicated by overlapping substrate specificity between enzymes from different gene families. To establish the role of the hepatic and extrahepatic P450 system in drug and foreign chemical disposition, drug efficacy, and toxicity, we created a unique mouse model in which 30 cytochrome P450 genes from the Cyp2c, Cyp2d, and Cyp3a gene clusters have been deleted. Remarkably, despite a wide range of putative important endogenous functions, Cyp2c/2d/3a KO mice were viable and fertile, demonstrating that these genes have evolved primarily as detoxification enzymes. Although there was no overt phenotype, detailed examination showed Cyp2c/2d/3a KO mice had a smaller body size (15%) and larger livers (20%). Changes in hepatic morphology and a decreased blood glucose (30%) were also noted. A five-drug cocktail of cytochrome P450 isozyme probe substrates were used to evaluate changes in drug pharmacokinetics; marked changes were observed in either the pharmacokinetics or metabolites formed from Cyp2c, Cyp2d, and Cyp3a substrates, whereas the metabolism of the Cyp1a substrate caffeine was unchanged. Thus, Cyp2c/2d/3a KO mice provide a powerful model to study the in vivo role of the P450 system in drug metabolism and efficacy, as well as in chemical toxicity. Y1 - 2014 U6 - http://dx.doi.org/10.1124/dmd.114.057885 SN - 1521-009X VL - 42 IS - 6 SP - 1022 EP - 1030 PB - ASPET CY - Bethesda, Md. ER - TY - JOUR A1 - Scheer, Nico A1 - Balimane, Praveen A1 - Hayward, Michael D. A1 - Buechel, Sandra A1 - Kauselmann, Gunther A1 - Wolf, C. Roland T1 - Generation and Characterization of a Novel Multidrug Resistance Protein 2 Humanized Mouse Line JF - Drug Metabolism and Disposition N2 - The multidrug resistance protein (MRP) 2 is predominantly expressed in liver, intestine, and kidney, where it plays an important role in the excretion of a range of drugs and their metabolites or endogenous compounds into bile, feces, and urine. Mrp knockout [Mrp2(−/−)] mice have been used recently to study the role of MRP2 in drug disposition. Here, we describe the first generation and initial characterization of a mouse line humanized for MRP2 (huMRP2), which is nulled for the mouse Mrp2 gene and expresses the human transporter in the organs and cell types where MRP2 is normally expressed. Analysis of the mRNA expression for selected cytochrome P450 and transporter genes revealed no major changes in huMRP2 mice compared with wild-type controls. We show that human MRP2 is able to compensate functionally for the loss of the mouse transporter as demonstrated by comparable bilirubin levels in the humanized mice and wild-type controls, in contrast to the hyperbilirubinemia phenotype that is observed in MRP2(−/−) mice. The huMRP2 mouse provides a model to study the role of the human transporter in drug disposition and in assessing the in vivo consequences of inhibiting this transporter by compounds interacting with human MRP2. Y1 - 2012 U6 - http://dx.doi.org/10.1124/dmd.112.047605 SN - 1521-0111 VL - 40 IS - 11 SP - 2212 EP - 2218 PB - ASPET CY - Bethesda, Md. ER - TY - JOUR A1 - Scheer, Nico A1 - Kapelyukh, Yury A1 - Rode, Anja A1 - Buechel, Sandra A1 - Wolf, C. Roland T1 - Generation and characterization of novel cytochrome P450 Cyp2c gene cluster knockout and CYP2C9 humanized mouse lines JF - Molecular Pharmacology N2 - Compared with rodents and many other animal species, the human cytochrome P450 (P450) Cyp2c gene cluster varies significantly in the multiplicity of functional genes and in the substrate specificity of its enzymes. As a consequence, the use of wild-type animal models to predict the role of human CYP2C enzymes in drug metabolism and drug-drug interactions is limited. Within the human CYP2C cluster CYP2C9 is of particular importance, because it is one of the most abundant P450 enzymes in human liver, and it is involved in the metabolism of a wide variety of important drugs and environmental chemicals. To investigate the in vivo functions of cytochrome P450 Cyp2c genes and to establish a model for studying the functions of CYP2C9 in vivo, we have generated a mouse model with a deletion of the murine Cyp2c gene cluster and a corresponding humanized model expressing CYP2C9 specifically in the liver. Despite the high number of functional genes in the mouse Cyp2c cluster and the reported roles of some of these proteins in different biological processes, mice deleted for Cyp2c genes were viable and fertile but showed certain phenotypic alterations in the liver. The expression of CYP2C9 in the liver also resulted in viable animals active in the metabolism and disposition of a number of CYP2C9 substrates. These mouse lines provide a powerful tool for studying the role of Cyp2c genes and of CYP2C9 in particular in drug disposition and as a factor in drug-drug interaction. Y1 - 2012 U6 - http://dx.doi.org/10.1124/mol.112.080036 SN - 1521-0111 VL - 82 IS - 6 SP - 1022 EP - 1029 PB - ASPET CY - Bethesda, Md. ER - TY - JOUR A1 - Lempiäinen, Harri A1 - Couttet, Philippe A1 - Bolognani, Federico A1 - Müller, Arne A1 - Dubost, Valérie A1 - Luisier, Raphaëlle A1 - Rio-Espinola, Alberto del A1 - Vitry, Veronique A1 - Unterberger, Elif B. A1 - Thomson, John P. A1 - Treindl, Fridolin A1 - Metzger, Ute A1 - Wrzodek, Clemens A1 - Hahne, Florian A1 - Zollinger, Tulipan A1 - Brasa, Sarah A1 - Kalteis, Magdalena A1 - Marcellin, Magali A1 - Giudicelli, Fanny A1 - Braeuning, Albert A1 - Morawiec, Laurent A1 - Zamurovic, Natasa A1 - Längle, Ulrich A1 - Scheer, Nico A1 - Schübeler, Dirk A1 - Goodman, Jay A1 - Chibout, Salah-Dine A1 - Marlowe, Jennifer A1 - Theil, Dietlinde A1 - Heard, David J. A1 - Grenet, Olivier A1 - Zell, Andreas A1 - Templin, Markus F. A1 - Meehan, Richard R. A1 - Wolf, Roland C. A1 - Elcombe, Clifford R. A1 - Schwarz, Michael A1 - Moulin, Pierre A1 - Terranova, Rémi A1 - Moggs, Jonathan G. T1 - Identification of Dlk1-Dio3 imprinted gene cluster non-coding RNAs as novel candidate biomarkers for liver tumor promotion JF - Toxicological Sciences N2 - The molecular events during nongenotoxic carcinogenesis and their temporal order are poorly understood but thought to include long-lasting perturbations of gene expression. Here, we have investigated the temporal sequence of molecular and pathological perturbations at early stages of phenobarbital (PB) mediated liver tumor promotion in vivo. Molecular profiling (mRNA, microRNA [miRNA], DNA methylation, and proteins) of mouse liver during 13 weeks of PB treatment revealed progressive increases in hepatic expression of long noncoding RNAs and miRNAs originating from the Dlk1-Dio3 imprinted gene cluster, a locus that has recently been associated with stem cell pluripotency in mice and various neoplasms in humans. PB induction of the Dlk1-Dio3 cluster noncoding RNA (ncRNA) Meg3 was localized to glutamine synthetase-positive hypertrophic perivenous hepatocytes, sug- gesting a role for β-catenin signaling in the dysregulation of Dlk1-Dio3 ncRNAs. The carcinogenic relevance of Dlk1-Dio3 locus ncRNA induction was further supported by in vivo genetic dependence on constitutive androstane receptor and β-catenin pathways. Our data identify Dlk1-Dio3 ncRNAs as novel candidate early biomarkers for mouse liver tumor promotion and provide new opportunities for assessing the carcinogenic potential of novel compounds. Y1 - 2012 U6 - http://dx.doi.org/10.1093/toxsci/kfs303 SN - 1094-2025 VL - 131 IS - 2 SP - 375 EP - 386 PB - Oxford University Press CY - Oxford ER - TY - JOUR A1 - Kapelyukh, Yury A1 - Henderson, Colin James A1 - Scheer, Nico A1 - Rode, Anja A1 - Wolf, Charles Roland T1 - Defining the contribution of CYP1A1 and CYP1A2 to drug metabolism using humanized CYP1A1/1A2 and Cyp1a1/Cyp1a2 KO mice JF - Drug Metabolism and Disposition Y1 - 2019 U6 - http://dx.doi.org/10.1124/dmd.119.087718 IS - Early view ER - TY - CHAP A1 - Hoffmann, Katharina A1 - Nieren, Monika A1 - Gäb, Martina A1 - Kasper, Anna A1 - Elbers, Gereon T1 - The potential of near infrared spectroscopy (NIRS) for the environmental biomonitoring of plants T2 - International conference on Life Sciences and Technology N2 - In the current environmental condition, the increase in pollution of the air, water, and soil indirectly will induce plants stress and decrease vegetation growth rate. These issues pay more attention to be solved by scientists worldwide. The higher level of chemical pollutants also induced the gradual changes in plants metabolism and decreased enzymatic activity. Importantly, environmental biomonitoring may play a pivotal contribution to prevent biodiversity degradation and plants stress due to pollutant exposure. Several previous studies have been done to monitor the effect of environmental changes on plants growth. Among that, Near Infrared spectroscopy (NIRS) offers an alternative way to observe the significant alteration of plant physiology caused by environmental damage related to pollution. Impairment of photosynthesis, nutrient and oxidative imbalances, and mutagenesis. Y1 - 2019 U6 - http://dx.doi.org/10.1088/1755-1315/276/1/012009 SN - 1755-1315 N1 - IOP Conference Series: Earth and Environmental Science : 276 VL - 276 IS - 012009 SP - 1 EP - 3 ER - TY - JOUR A1 - Schiffels, Johannes A1 - Selmer, Thorsten T1 - Combinatorial assembly of ferredoxin‐linked modules in Escherichia coli yields a testing platform for Rnf‐complexes JF - Biotechnology and Bioengineering Y1 - 2019 U6 - http://dx.doi.org/10.1002/bit.27079 IS - accepted article SP - 1 EP - 36 PB - Wiley CY - Weinheim ER - TY - JOUR A1 - Scheer, Nico A1 - Henderson, Colin James A1 - Kapelyukh, Yury A1 - Rode, Anja A1 - Mclaren, Aileen W. A1 - MacLeod, Alastair Kenneth A1 - Lin, De A1 - Wright, Jayne A1 - Stanley, Lesley A1 - Wolf, C. Roland T1 - An extensively humanised mouse model to predict pathways of drug disposition, drug/drug interactions, and to facilitate the design of clinical trials JF - Drug Metabolism and Disposition Y1 - 2019 U6 - http://dx.doi.org/10.1124/dmd.119.086397 IS - Early view ER - TY - JOUR A1 - Delaittre, Guillaume T1 - Telechelic Poly(2-Oxazoline)s JF - European Polymer Journal Y1 - 2019 U6 - http://dx.doi.org/10.1016/j.eurpolymj.2019.109281 SN - 0014-3057 IS - In Press, Journal Pre-proof, 109281 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Engel, Mareike A1 - Gemünde, Andre A1 - Holtmann, Dirk A1 - Müller-Renno, Christine A1 - Ziegler, Christiane A1 - Tippkötter, Nils A1 - Ulber, Roland T1 - Clostridium acetobutylicum’s connecting world: cell appendage formation in bioelectrochemical systems JF - ChemElectroChem Y1 - 2019 U6 - http://dx.doi.org/10.1002/celc.201901656 SN - 2196-0216 IS - Accepted Article PB - Wiley CY - Weinheim ER - TY - JOUR A1 - Tippkötter, Nils A1 - Roth, Jasmine T1 - Purified Butanol from Lignocellulose – Solvent‐Impregnated Resins for an Integrated Selective Removal JF - Chemie Ingenieur Technik N2 - In traditional microbial biobutanol production, the solvent must be recovered during fermentation process for a sufficient space-time yield. Thermal separation is not feasible due to the boiling point of n-butanol. As an integrated and selective solid-liquid separation alternative, solvent impregnated resins (SIRs) were applied. Two polymeric resins were evaluated and an extractant screening was conducted. Vacuum application with vapor collection in fixed-bed column as bioreactor bypass was successfully implemented as butanol desorption step. In course of further increasing process economics, fermentation with renewable lignocellulosic substrates was conducted using Clostridium acetobutylicum. Utilization of SIR was shown to be a potential strategy for solvent removal from fermentation broth, while application of a bypass column allows for product removal and recovery at once. KW - Biofuel KW - Biorefinery KW - Butanol KW - Clostridium acetobutylicum KW - Lignocellulose Y1 - 2020 U6 - http://dx.doi.org/10.1002/cite.202000200 SN - 1522-2640 VL - 92 IS - 11 SP - 1741 EP - 1751 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Scheer, Nico A1 - Wilson, Ian D. T1 - A comparison between genetically humanized and chimeric liver humanized mouse models for studies in drug metabolism and toxicity JF - Drug Discovery Today N2 - Mice that have been genetically humanized for proteins involved in drug metabolism and toxicity and mice engrafted with human hepatocytes are emerging and promising in vivo models for an improved prediction of the pharmacokinetic, drug–drug interaction and safety characteristics of compounds in humans. The specific advantages and disadvantages of these models should be carefully considered when using them for studies in drug discovery and development. Here, an overview on the corresponding genetically humanized and chimeric liver humanized mouse models described to date is provided and illustrated with examples of their utility in drug metabolism and toxicity studies. We compare the strength and weaknesses of the two different approaches, give guidance for the selection of the appropriate model for various applications and discuss future trends and perspectives. Y1 - 2016 U6 - http://dx.doi.org/10.1016/j.drudis.2015.09.002 SN - 1359-6446 VL - 21 IS - 2 SP - 250 EP - 263 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Scheer, Nico A1 - Wolf, C. Roland T1 - Genetically humanized mouse models of drug metabolizing enzymes and transporters and their applications JF - Xenobiotica N2 - 1. Drug metabolizing enzymes and transporters play important roles in the absorption, metabolism, tissue distribution and excretion of various compounds and their metabolites and thus can significantly affect their efficacy and safety. Furthermore, they can be involved in drug–drug interactions which can result in adverse responses, life-threatening toxicity or impaired efficacy. Significant species differences in the interaction of compounds with drug metabolizing enzymes and transporters have been described. 2. In order to overcome the limitation of animal models in accurately predicting human responses, a large variety of mouse models humanized for drug metabolizing enzymes and to a lesser extent drug transporters have been created. 3. This review summarizes the literature describing these mouse models and their key applications in studying the role of drug metabolizing enzymes and transporters in drug bioavailability, tissue distribution, clearance and drug–drug interactions as well as in human metabolite testing and risk assessment. 4. Though such humanized mouse models have certain limitations, there is great potential for their use in basic research and for testing and development of new medicines. These limitations and future potentials will be discussed. KW - transporters KW - human metabolites KW - drug metabolising enzymes KW - drug–drug interactions KW - bioavailability Y1 - 2014 U6 - http://dx.doi.org/10.3109/00498254.2013.815831 SN - 1366-5928 VL - 44 IS - 2 SP - 96 EP - 108 PB - Taylor & Francis CY - Abingdon ER - TY - JOUR A1 - Scheer, Nico A1 - Wolf, C. Roland T1 - Xenobiotic receptor humanized mice and their utility JF - Drug Metabolism Reviews Y1 - 2013 U6 - http://dx.doi.org/10.3109/03602532.2012.738687 SN - 1097-9883 IS - 1 SP - 110 EP - 121 PB - Taylor & Francis CY - London ER - TY - JOUR A1 - Henderson, Colin J. A1 - Scheer, Nico A1 - Wolf, C. Roland T1 - Advances in the generation of mouse models to elucidate the pathways of drug metabolism in rodents and man JF - Expert Review of Clinical Pharmacology Y1 - 2009 U6 - http://dx.doi.org/10.1586/17512433.2.2.105 SN - 1751-2441 VL - 2 IS - 2 SP - 105 EP - 109 PB - Taylor & Francis CY - London ER - TY - JOUR A1 - Stanley, Lesley A. A1 - Horsburgh, Brian C. A1 - Ross, Jillian A1 - Scheer, Nico A1 - Wolf, C. Roland T1 - Drug transporters: Gatekeepers controlling access of xenobiotics to the cellular interior JF - Drug Metabolism Reviews Y1 - 2009 U6 - http://dx.doi.org/10.1080/03602530802605040 SN - 1097-9883 VL - 41 IS - 1 SP - 27 EP - 65 PB - Taylor & Francis CY - London ER - TY - JOUR A1 - Stanley, Lesley A. A1 - Horsburgh, Brian C. A1 - Ross, Jillian A1 - Scheer, Nico A1 - Wolf, C. Roland T1 - Nuclear Receptors which play a pivotal role in drug disposition and chemical toxicity JF - Drug Metabolism Reviews Y1 - 2006 U6 - http://dx.doi.org/10.1080/03602530600786232 SN - 1097-9883 VL - 38 IS - 3 SP - 515 EP - 597 ER - TY - CHAP A1 - Samuelsson, K. A1 - Scheer, Nico A1 - Wilson, I. A1 - Wolf, C.R. A1 - Henderson, C.J. ED - Chackalamannil, Samuel T1 - Genetically Humanized Animal Models T2 - Comprehensive Medicinal Chemistry III. 3rd Edition N2 - Genetically humanized mice for proteins involved in drug metabolism and toxicity and mice engrafted with human hepatocytes are emerging as promising in vivo models for improved prediction of the pharmacokinetic, drug–drug interaction, and safety characteristics of compounds in humans. This is an overview on the genetically humanized and chimeric liver-humanized mouse models, which are illustrated with examples of their utility in drug metabolism and toxicity studies. The models are compared to give guidance for selection of the most appropriate model by highlighting advantages and disadvantages to be carefully considered when used for studies in drug discovery and development. KW - Chimeric liver-humanized mice KW - Drug distribution KW - Drug metabolism KW - Toxicology KW - Knockout mice Y1 - 2017 SN - 978-0-12-803201-5 U6 - http://dx.doi.org/10.1016/B978-0-12-409547-2.12376-5 SP - 130 EP - 149 PB - Elsevier CY - Saint Louis ER - TY - CHAP A1 - Scheer, Nico A1 - Chu, Xiaoyan A1 - Salphati, Laurent A1 - Zamek-Gliszczynski, Maciej J. ED - Nicholls, Glynis T1 - Knockout and humanized animal models to study membrane transporters in drug development T2 - Drug Transporters: Volume 1: Role and Importance in ADME and Drug Development Y1 - 2016 SN - 978-1-78262-379-3 U6 - http://dx.doi.org/10.1039/9781782623793-00298 SP - 298 EP - 332 PB - Royal Society of Chemistry CY - Cambridge ER - TY - CHAP A1 - Wolf, C. Roland A1 - Kapelyukh, Yury A1 - Scheer, Nico A1 - Henderson, Colin J. ED - Wilson, Alan G. E. T1 - Application of Humanised and Other Transgenic Models to Predict Human Responses to Drugs N2 - The use of transgenic animal models has transformed our knowledge of complex biochemical pathways in vivo. It has allowed disease processes to be modelled and used in the development of new disease prevention and treatment strategies. They can also be used to define cell- and tissue-specific pathways of gene regulation. A further major application is in the area of preclinical development where such models can be used to define pathways of chemical toxicity, and the pathways that regulate drug disposition. One major application of this approach is the humanisation of mice for the proteins that control drug metabolism and disposition. Such models can have numerous applications in the development of drugs and in their more sophisticated use in the clinic. Y1 - 2015 SN - 978-1-78262-778-4 U6 - http://dx.doi.org/10.1039/9781782622376-00152 SP - 152 EP - 176 PB - RSC Publ. CY - Cambridge ER - TY - CHAP A1 - Henderson, Colin J. A1 - Wolf, C. Roland A1 - Scheer, Nico ED - Woolf, Thomas F. T1 - The use of transgenic animals to study drug metabolism T2 - Handbook of Drug Metabolism. 2nd Edition Y1 - 2009 SN - 978-1-4200-7647-9 SP - 637 EP - 658 PB - Informa Healthcare CY - New York ER - TY - JOUR A1 - Engel, Mareike A1 - Bayer, Hendrik A1 - Holtmann, Dirk A1 - Tippkötter, Nils A1 - Ulber, Roland T1 - Flavin secretion of Clostridium acetobutylicum in a bioelectrochemical system - Is an iron limitation involved? JF - Bioelectrochemistry Y1 - 2019 U6 - http://dx.doi.org/10.1016/j.bioelechem.2019.05.014 SN - 1567-5394 IS - In Press, Accepted Manuscript PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Scheer, Nico A1 - Ross, Jillian A1 - Rode, Anja A1 - Zevnik, Branko A1 - Niehaves, Sandra A1 - Faust, Nicole A1 - Wolf, C. Roland T1 - A novel panel of mouse models to evaluate the role of human pregnane X receptor and constitutive androstane receptor in drug response JF - Journal of Clinical Investigation Y1 - 2008 U6 - http://dx.doi.org/https://doi.org/10.1172/JCI35483 SN - 1558-8238 VL - 118 IS - 9 SP - 3228 EP - 3239 ER - TY - JOUR A1 - Scheer, Nico A1 - Kapelyukh, Yury A1 - McEwan, Jillian A1 - Beuger, Vincent A1 - Stanley, Lesley A. A1 - Rode, Anja A1 - Wolf, C. Roland T1 - Modeling Human Cytochrome P450 2D6 Metabolism and Drug-drug Interaction by a Novel Panel of Knockout and Humanized Mouse Lines JF - Molecular Pharmacology N2 - The highly polymorphic human cytochrome P450 2D6 enzyme is involved in the metabolism of up to 25% of all marketed drugs and accounts for significant individual differences in response to CYP2D6 substrates. Because of the differences in the multiplicity and substrate specificity of CYP2D family members among species, it is difficult to predict pathways of human CYP2D6-dependent drug metabolism on the basis of animal studies. To create animal models that reflect the human situation more closely and that allow an in vivo assessment of the consequences of differential CYP2D6 drug metabolism, we have developed a novel straightforward approach to delete the entire murine Cyp2d gene cluster and replace it with allelic variants of human CYP2D6. By using this approach, we have generated mouse lines expressing the two frequent human protein isoforms CYP2D6.1 and CYP2D6.2 and an as yet undescribed variant of this enzyme, as well as a Cyp2d cluster knockout mouse. We demonstrate that the various transgenic mouse lines cover a wide spectrum of different human CYP2D6 metabolizer phenotypes. The novel humanization strategy described here provides a robust approach for the expression of different CYP2D6 allelic variants in transgenic mice and thus can help to evaluate potential CYP2D6-dependent interindividual differences in drug response in the context of personalized medicine. Y1 - 2012 U6 - http://dx.doi.org/10.1124/mol.111.075192 SN - 1521-0111 VL - 81 IS - 1 SP - 63 EP - 72 PB - ASPET CY - Bethesda, Md. ER - TY - JOUR A1 - Reugels, Alexander M. A1 - Boggetti, Barbara A1 - Scheer, Nico A1 - Campos-Ortega, José A. T1 - Asymmetric localization of Numb:EGFP in dividing neuroepithelial cells during neurulation in Danio rerio JF - Developmental Dynamics Y1 - 2006 U6 - http://dx.doi.org/10.1002/dvdy.20699 SN - 1097-0177 VL - 235 IS - 4 SP - 934 EP - 948 ER - TY - JOUR A1 - Hans, Stefan A1 - Scheer, Nico A1 - Riedl, Iris A1 - Weizäcker, Elisabeth von A1 - Blader, Patrick A1 - Campos-Ortega, José A. T1 - her3, a zebrafish member of the hairy-E(spl) family, is repressed by Notch signalling JF - Development Y1 - 2004 U6 - http://dx.doi.org/10.1242/dev.01167 SN - 1477-9129 VL - 131 IS - 12 SP - 2957 EP - 2969 ER - TY - JOUR A1 - Scheer, Nico A1 - Riedl, Iris A1 - Warren, J.T. A1 - Kuwada, John Y. A1 - Campos-Ortega, José A. T1 - A quantitative analysis of the kinetics of Gal4 activator and effector gene expression in the zebrafish JF - Mechanism of Development Y1 - 2002 U6 - http://dx.doi.org/10.1016/S0925-4773(01)00621-9 SN - 0925-4773 VL - 112 IS - 1-2 SP - 9 EP - 14 ER - TY - JOUR A1 - Lawson, Nathan D. A1 - Scheer, Nico A1 - Pham, Van N. A1 - Kim, Ceol-Hee A1 - Chitnis, Ajay B. A1 - Campos-Ortega, José A. A1 - Weinstein, Brant M. T1 - Notch signaling is required for arterial-venous differentiation during embryonic vascular development JF - Development Y1 - 2001 SN - 1477-9129 VL - 128 IS - 19 SP - 3675 EP - 3683 ER - TY - JOUR A1 - Scheer, Nico A1 - Groth, Anne A1 - Hans, Stefan A1 - Campos-Ortega, José A. T1 - An instructive function for Notch in promoting gliogenesis in the zebrafish retina JF - Development Y1 - 2001 SN - 0950-1991 VL - 128 IS - 7 SP - 1099 EP - 1107 ER - TY - JOUR A1 - Halbach, Thorsten A1 - Scheer, Nico T1 - Transcriptional activation by the PHD finger is inhibited through an adjacent leucine zipper that binds 14-3-3 proteins JF - Nucleic Acids Research Y1 - 2000 U6 - http://dx.doi.org/10.1093/nar/28.18.3542 SN - 1362-4962 VL - 28 IS - 18 SP - 3542 EP - 3550 ER - TY - JOUR A1 - Scheer, Nico A1 - Campos-Ortega, José A. T1 - Use of the Gal4-UAS technique for targeted gene expression in the zebrafish JF - Mechanism of Development Y1 - 1999 U6 - http://dx.doi.org/10.1016/S0925-4773(98)00209-3 SN - 0925-4773 VL - 80 IS - 2 SP - 153 EP - 158 ER - TY - PAT A1 - Friederici, Nadja-Annette A1 - Hojdis, Nils T1 - Fahrzeugreifen N2 - Die Erfindung betrifft einen Fahrzeugreifen mit zumindest einem radial außen befindlichen Gummilaufstreifen, Wulstbereichen für den Anschluss an eine Felge und mit auf einer Gummimischung basierenden Seitenwandbereichen zwischen Gummilaufstreifen und den Wulstbereichen. Ferner betrifft die Erfindung Verfahren zur Herstellung solcher Fahrzeugreifen. Um den Anteil der umweltschädlichen Substanzen, insbesondere im Innenstadtbereich, zu reduzieren weisen die Seitenwandbereiche auf der äußeren Oberfläche eine für den oxidativen Abbau von Molekülen photokatalytisch aktive Substanz auf. Y1 - 2018 N1 - Patent DE102016219338A1 04.12.2018 ER - TY - PAT A1 - Hojdis, Nils A1 - Multhaupt, Hendrik A1 - Recker, Carla A1 - Wark, Michael T1 - Schwefelvernetzbare Kautschukmischung, Vulkanisat der Kautschukmischung und Fahrzeugreifen N2 - Die Erfindung betrifft eine schwefelvernetzbare Kautschukmischung, deren Vulkanisat und einen Fahrzeugreifen. Die erfindungsgemäße Kautschukmischung enthält wenigstens folgende Bestandteile: - Wenigstens einen Dienkautschuk; - wenigstens eine Kohle (HTC-Kohle), die mittels hydrothermaler Karbonisierung von wenigstens einer Ausgangssubstanz hergestellt ist. Der erfindungsgemäße Fahrzeugreifen weist in wenigstens einem Bauteil wenigstens ein erfindungsgemäßes Vulkanisat der Kautschukmischung auf. Y1 - 2019 N1 - Patent EP3470457A1 17.04.2019 auch veröffentlicht unter WO2019072431A1 ER - TY - PAT A1 - Dauer, David-Raphael A1 - Hojdis, Nils A1 - Müller, Norbert A1 - Recker, Carla A1 - Schax, Fabian A1 - Schöffel, Julia A1 - Tarantola, Gesa A1 - Weber, Christine T1 - Schwefelvernetzbare Kautschukmischung, Vulkanisat der Kautschukmischung und Fahrzeugreifen N2 - Die Erfindung betrifft eine schwefelvernetzbare Kautschukmischung, deren Vulkanisat und einen Fahrzeugreifen. Die schwefelvernetzbare Kautschukmischung enthält wenigstens die folgenden Bestandteile: - wenigstens einen Dienkautschuk; und - 10 bis 300 phr wenigstens einer Kieselsäure; und - 1 bis 30 phf wenigstens eines Silans A mit der allgemeinen Summenformel A-I) A-I)(R1)oSi-R2-(S-R3)q-S-X; und - 0,5 bis 30 phf wenigstens eines Silans B mit der allgemeinen Summenformel B-I) B-I) (R1)oSi-R2-(S-R3)u-S-R2-Si(R1)o wobei q =1, 2 oder 3 ist; und u = 1, 2 oder 3 ist; und X ein Wasserstoffatom oder eine –C(=O)-R8 Gruppe ist wobei R8 ausgewählt ist aus Wasserstoff, C1-C20 Alkylgruppen, vorzugsweise C1-C17, C6-C20- Arylgruppen, vorzugsweise Phenyl, C2-C20-Alkenylgruppen und C7-C20-Aralkylgruppen. Y1 - 2019 N1 - Patent WO2019105614A1 06.06.2019 auch veröffentlicht unter CN111386199A; DE102017221232A1; TW201925306A ER - TY - JOUR A1 - Moret, J.L.T.M. A1 - Alkemade, J. A1 - Upcraft, T.M. A1 - Paulßen, Elisabeth A1 - Wolterbeek, H.T. A1 - Ommen, J.R. van A1 - Denkova, A.G. T1 - The application of atomic layer deposition in the production of sorbents for ⁹⁹Mo/⁹⁹ᵐTc generator JF - Applied Radiation and Isotopes N2 - New production routes for ⁹⁹Mo are steadily gaining importance. However, the obtained specific activity is much lower than currently produced by the fission of U-235. To be able to supply hospitals with ⁹⁹Mo/⁹⁹ᵐTc generators with the desired activity, the adsorption capacity of the column material should be increased. In this paper we have investigated whether the gas phase coating technique Atomic Layer Deposition (ALD), which can deposit ultra-thin layers on high surface area materials, can be used to attain materials with high adsorption capacity for ⁹⁹Mo. For this purpose, ALD was applied on a silica-core sorbent material to coat it with a thin layer of alumina. This sorbent material shows to have a maximum adsorption capacity of 120 mg/g and has a ⁹⁹ᵐTc elution efficiency of 55 ± 2% based on 3 executive elutions. Y1 - 2020 U6 - http://dx.doi.org/10.1016/j.apradiso.2020.109266 SN - 0969-8043 VL - 164 IS - 109266 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Schiedermeier, Maximilian A1 - Rettner, Cornelius A1 - Heilmann, Marcel A1 - Schneider, Felix A1 - Marz, Martin T1 - Interference of automotive HV-DC-systems by traction voltage-source-inverters (VSI) JF - 2019 IEEE Transportation Electrification Conference (ITEC-India) Y1 - 2019 U6 - http://dx.doi.org/10.1109/ITEC-India48457.2019.ITECINDIA2019-37 SP - 1 EP - 6 PB - IEEE CY - New York ER - TY - JOUR A1 - El Moussaoui, Noureddine A1 - Talbi, Sofian A1 - Atmane, Ilyas A1 - Kassmi, Khalil A1 - Schwarzer, Klemens A1 - Chayeb, Hamid A1 - Bachiri, Najib T1 - Feasibility of a new design of a Parabolic Trough Solar Thermal Cooker (PSTC) JF - Solar Energy N2 - In this article, we describe the structure, the functioning, and the tests of parabolic trough solar thermal cooker (PSTC). This oven is designed to meet the needs of rural residents, including Urban, which requires stable cooking temperatures above 200 °C. The cooking by this cooker is based on the concentration of the sun's rays on a glass vacuum tube and heating of the oil circulate in a big tube, located inside the glass tube. Through two small tubes, associated with large tube, the heated oil, rise and heats the pot of cooking pot containing the food to be cooked (capacity of 5 kg). This cooker is designed in Germany and extensively tested in Morocco for use by the inhabitants who use wood from forests. During a sunny day, having a maximum solar radiation around 720 W/m2 and temperature ambient around 26 °C, maximum temperatures recorded of the small tube, the large tube and the center of the pot are respectively: 370 °C, 270 °C and 260 °C. The cooking process with food at high (fries, ..), we show that the cooking oil temperature rises to 200 °C, after 1 h of heating, the cooking is done at a temperature of 120 °C for 20 min. These temperatures are practically stable following variations and decreases in the intensity of irradiance during the day. The comparison of these results with those of the literature shows an improvement of 30–50 % on the maximum value of the temperature with a heat storage that could reach 60 min of autonomy. All the results obtained show the good functioning of the PSTC and the feasibility of cooking food at high temperature (>200 °C). Y1 - 2020 U6 - http://dx.doi.org/10.1016/j.solener.2020.03.079 SN - 0038-092X VL - 201 IS - Vol. 201 (May 2020) SP - 866 EP - 871 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Muschallik, Lukas A1 - Kipp, Carina Ronja A1 - Recker, Inga A1 - Bongaerts, Johannes A1 - Pohl, Martina A1 - Gelissen, Melanie A1 - Schöning, Michael Josef A1 - Selmer, Thorsten A1 - Siegert, Petra T1 - Synthesis of α-hydroxy ketones and vicinal diols with the Bacillus licheniformis DSM 13T butane-2, 3-diol dehydrogenase JF - Journal of Biotechnology N2 - The enantioselective synthesis of α-hydroxy ketones and vicinal diols is an intriguing field because of the broad applicability of these molecules. Although, butandiol dehydrogenases are known to play a key role in the production of 2,3-butandiol, their potential as biocatalysts is still not well studied. Here, we investigate the biocatalytic properties of the meso-butanediol dehydrogenase from Bacillus licheniformis DSM 13T (BlBDH). The encoding gene was cloned with an N-terminal StrepII-tag and recombinantly overexpressed in E. coli. BlBDH is highly active towards several non-physiological diketones and α-hydroxyketones with varying aliphatic chain lengths or even containing phenyl moieties. By adjusting the reaction parameters in biotransformations the formation of either the α-hydroxyketone intermediate or the diol can be controlled. Y1 - 2020 SN - 2590-1559 U6 - http://dx.doi.org/10.1016/j.jbiotec.2020.09.016 VL - 202 IS - Vol. 324 SP - 61 EP - 70 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Mayer, Jan A1 - Hentschke, Reinhard A1 - Hager, Jonathan A1 - Hojdis, Nils A1 - Karimi-Varnaneh, Hossein Ali T1 - A Nano-Mechanical Instability as Primary Contribution to Rolling Resistance JF - Scientific Reports Y1 - 2017 SN - 2045-2322 VL - 7 IS - Article number 11275 PB - Springer CY - Berlin ER - TY - JOUR A1 - Svaneborg, Carsten A1 - Karimi-Varzaneh, Hossein Ali A1 - Hojdis, Nils A1 - Fleck, Franz A1 - Everaers, Ralf T1 - Multiscale approach to equilibrating model polymer melts JF - Physical Review E N2 - We present an effective and simple multiscale method for equilibrating Kremer Grest model polymer melts of varying stiffness. In our approach, we progressively equilibrate the melt structure above the tube scale, inside the tube and finally at the monomeric scale. We make use of models designed to be computationally effective at each scale. Density fluctuations in the melt structure above the tube scale are minimized through a Monte Carlo simulated annealing of a lattice polymer model. Subsequently the melt structure below the tube scale is equilibrated via the Rouse dynamics of a force-capped Kremer-Grest model that allows chains to partially interpenetrate. Finally the Kremer-Grest force field is introduced to freeze the topological state and enforce correct monomer packing. We generate 15 melts of 500 chains of 10.000 beads for varying chain stiffness as well as a number of melts with 1.000 chains of 15.000 monomers. To validate the equilibration process we study the time evolution of bulk, collective, and single-chain observables at the monomeric, mesoscopic, and macroscopic length scales. Extension of the present method to longer, branched, or polydisperse chains, and/or larger system sizes is straightforward. Y1 - 2016 U6 - http://dx.doi.org/10.1103/PhysRevE.94.032502 SN - 2470-0053 VL - 94 IS - 032502 PB - AIP Publishing CY - Melville, NY ER - TY - JOUR A1 - Schwab, Lukas A1 - Hojdis, Nils A1 - Lacayo, Jorge A1 - Wilhelm, Manfred T1 - Fourier-Transform Rheology of Unvulcanized, Carbon Black Filled Styrene Butadiene Rubber JF - Macromolecular Materials and Engineering N2 - Rubber materials filled with reinforcing fillers display nonlinear rheological behavior at small strain amplitudes below γ0 < 0.1. Nevertheless, rheological data are analyzed mostly in terms of linear parameters, such as shear moduli (G′, G″), which loose their physical meaning in the nonlinear regime. In this work styrene butadiene rubber filled with carbon black (CB) under large amplitude oscillatory shear (LAOS) is analyzed in terms of the nonlinear parameter I3/1. Three different CB grades are used and the filler load is varied between 0 and 70 phr. It is found that I3/1(φ) is most sensitive to changes of the total accessible filler surface area at low strain amplitudes (γ0 = 0.32). The addition of up to 70 phr CB leads to an increase of I3/1(φ) by a factor of more than ten. The influence of the measurement temperature on I3/1 is pronounced for CB levels above the percolation threshold. Y1 - 2016 U6 - http://dx.doi.org/10.1002/mame.201500356 SN - 1439-2054 VL - 301 IS - 4 SP - 457 EP - 468 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Harish, Ajay B. A1 - Wriggers, Peter A1 - Jungk, Juliane A1 - Hojdis, Nils A1 - Recker, Carla T1 - Mesoscale Constitutive Modeling of Non-Crystallizing Filled Elastomers JF - Computational Mechanics N2 - Elastomers are exceptional materials owing to their ability to undergo large deformations before failure. However, due to their very low stiffness, they are not always suitable for industrial applications. Addition of filler particles provides reinforcing effects and thus enhances the material properties that render them more versatile for applications like tyres etc. However, deformation behavior of filled polymers is accompanied by several nonlinear effects like Mullins and Payne effect. To this day, the physical and chemical changes resulting in such nonlinear effect remain an active area of research. In this work, we develop a heterogeneous (or multiphase) constitutive model at the mesoscale explicitly considering filler particle aggregates, elastomeric matrix and their mechanical interaction through an approximate interface layer. The developed constitutive model is used to demonstrate cluster breakage, also, as one of the possible sources for Mullins effect observed in non-crystallizing filled elastomers. Y1 - 2016 U6 - http://dx.doi.org/10.1007/s00466-015-1251-1 SN - 1432-0924 VL - 57 SP - 653 EP - 677 PB - Springer CY - Berlin ER - TY - JOUR A1 - Hentschke, Reinhard A1 - Hager, Jonathan A1 - Hojdis, Nils T1 - Molecular Modeling Approach to the Prediction of Mechanical Properties of Silica-Reinforced Rubbers JF - Journal of Applied Polymer Science N2 - Recently, we have suggested a nanomechanical model for dissipative loss in filled elastomer networks in the context of the Payne effect. The mechanism is based on a total interfiller particle force exhibiting an intermittent loop, due to the combination of short-range repulsion and dispersion forces with a long-range elastic attraction. The sum of these forces leads, under external strain, to a spontaneous instability of “bonds” between the aggregates in a filler network and attendant energy dissipation. Here, we use molecular dynamics simulations to obtain chemically realistic forces between surface modified silica particles. The latter are combined with the above model to estimate the loss modulus and the low strain storage modulus in elastomers containing the aforementioned filler-compatibilizer systems. The model is compared to experimental dynamic moduli of silica filled rubbers. We find good agreement between the model predictions and the experiments as function of the compatibilizer's molecular structure and its bulk concentration. KW - theory and modeling KW - supramolecular structures KW - rubber KW - mechanical properties KW - elastomers Y1 - 2014 U6 - http://dx.doi.org/10.1002/app.40806 SN - 1097-4628 VL - 131 IS - 18 SP - 1 EP - 9 PB - Wiley CY - New York, NY ER - TY - PAT A1 - Finger, Sebastian A1 - Hojdis, Nils A1 - Menglong, Huang T1 - Sekundäre galvanische Zelle N2 - Die vorliegende Erfindung betrifft eine sekundäre galvanische Zelle, umfassend eine Kathode, eine Anode und einen Separator, der zwischen der Kathode und der Anode angeordnet ist, wobei die Kathode ein erstes elastomeres Polymer umfasst, welches mit einem ersten Füllstoff als Kathodenmaterial gefüllt ist, wobei die Anode ein zweites elastomeres Polymer umfasst, welches mit einem zweiten Füllstoff als Anodenmaterial gefüllt ist, wobei der Separator ein drittes elastomeres Polymer umfasst, wobei das erste elastomere Polymer, das zweite elastomere Polymer und das dritte elastomere Polymer unabhängig voneinander aus vernetzungsfähigen Dienkautschuken ausgewählt sind, und wobei zumindest eines von dem ersten elastomeren Polymer, dem zweiten elastomeren Polymer und dem dritten elastomeren Polymer eine ionische Flüssigkeit und/oder ein elektrisch leitfähiges Polymer enthält. Die vorliegende Erfindung betrifft ferner eine wiederaufladbare Batterie, umfassend die erfindungsgemäße sekundäre galvanische Zelle, ein Verfahren zur Herstellung einer sekundären galvanischen Zelle sowie die Verwendung der erfindungsgemäßen sekundären galvanischen Zelle sowie der erfindungsgemäßen wiederaufladbaren Batterie. Y1 - 2020 N1 - Patent DE102018222385A1 25.06.2020 auch veröffentlicht unter EP3671932A1 ER - TY - PAT A1 - Hojdis, Nils A1 - Recker, Carla T1 - Schwefelvernetzte Kautschukmischung für Fahrzeugreifen enthaltend Carbon Nanotubes (cnt), Fahrzeugreifen, Der Die schwefelvernetzte Kautschukmischung aufweist, sowie Verfahren zur Herstellung der schwefelvernetzten Kautschukmischung enthaltend Cnt Y1 - 2020 N1 - Patent EP000003625288A1 25.03.2020 auch veröffentlicht unter CN110678508A; DE102017208137A1; WO2018210471A1 ER - TY - PAT A1 - Ehmke, Tobias A1 - Finger, Sebastian A1 - Hojdis, Nils A1 - Kurz, Martin A1 - Nawrocka-Herczynska, Monika T1 - Funkeinheit und Vorrichtung mit einer Funkeinheit N2 - Dargestellt und beansprucht ist eine Funkeinheit mit einem Funkbauteil, einer elektrisch leitfähigen Antenne und einem Kunststoffverbindungsmittel. Das Funkbauteil weist einen Antennenanschluss auf. Das Kunststoffverbindungsmittel weist ein erstes Elastomermaterial mit elektrisch leitfähigem Zusatzmaterial auf, so dass das Kunststoffverbindungsmittel elektrisch leitfähig ist. Das Kunststoffverbindungsmittel bildet eine mechanische und elektrisch leitfähige Verbindung zwischen dem Antennenanschluss und der Antenne. Weiterhin wird eine Vorrichtung mit einem ein Matrixmaterial mit oder aus einem zweiten Elastomermaterial und einer Funkeinheit beschrieben und beansprucht, wobei die Funkeinheit vollständig in das Matrixmaterial eingebettet ist. Die Vorrichtung kann insbesondere ein Fahrzeugreifen sein. Y1 - 2020 N1 - Patent DE102018222380A1 25.06.2020 ER - TY - PAT A1 - Hojdis, Nils T1 - Verfahren zum Bestimmen einer Sitzeinstellung eines Sitzes eines Kraftfahrzeugs, Computerprogrammprodukt, Servereinrichtung, Kommunikationsendgerät und Kraftfahrzeug N2 - Die Erfindung betrifft ein Verfahren zum Bestimmen einer Sitzeinstellung (23) eines Sitzes (3) eines Kraftfahrzeugs (1), bei welchem folgende Schritte durchgeführt werden:- Bereitstellen einer Körperabmessung (12) eines Nutzers (6) des Kraftfahrzeugs (1);- Bereitstellen einer Sitzeigenschaft (13) des Sitzes (3) des Kraftfahrzeugs (1);- Bereitstellen eines mit zumindest einer Referenzsitzeigenschaft (20), zumindest einer Referenzkörperabmessung (21) und zumindest einer Referenzsitzeinstellung (22) trainiertes künstliches neuronales Netz (14); und- Bestimmen der Sitzeinstellung (23) durch Eingabe der Körperabmessung (12) und der Sitzeigenschaft (13) in das künstliche neuronale Netz (14). Y1 - 2019 N1 - Patent DE102018211831A1 05.12.2019 ER - TY - PAT A1 - Dauer, David-Raphael A1 - Hojdis, Nils A1 - Müller, Norbert A1 - Recker, Carla A1 - Schax, Fabian A1 - Schöffel, Julia A1 - Tarantola, Gesa A1 - Weber, Christine T1 - Schwefelvernetzbare Kautschukmischung, Vulkanisat der Kautschukmischung und Fahrzeugreifen N2 - Die Erfindung betrifft eine schwefelvernetzbare Kautschukmischung, deren Vulkanisat und einen Fahrzeugreifen.Die schwefelvernetzbare Kautschukmischung enthält wenigstens die folgenden Bestandteile:- wenigstens einen Dienkautschuk; und- 10 bis 300 phr wenigstens einer Kieselsäure ; und- 1 bis 30 phf wenigstens eines Silans A mit der allgemeinen Summenformel A-I)und- 0,5 bis 30 phf wenigstens eines Silans B mit der allgemeinen Summenformel B-I)wobei u gleich 0, 1, 2 oder 3 und v gleich 0 oder 1 ist. Y1 - 2019 N1 - Patent DE102017221236A1 29.05.2019 auch veröffentlicht unter CN111405987A; TW201925305A; WO2019105615A1 ER - TY - JOUR A1 - Everaers, Ralf A1 - Karimi-Varzaneh, Hossein Ali A1 - Fleck, Franz A1 - Hojdis, Nils A1 - Svaneborg, Carsten T1 - Kremer–Grest Models for Commodity Polymer Melts: Linking Theory, Experiment, and Simulation at the Kuhn Scale JF - Macromolecules N2 - The Kremer–Grest (KG) polymer model is a standard model for studying generic polymer properties in molecular dynamics simulations. It owes its popularity to its simplicity and computational efficiency, rather than its ability to represent specific polymers species and conditions. Here we show that by tuning the chain stiffness it is possible to adapt the KG model to model melts of real polymers. In particular, we provide mapping relations from KG to SI units for a wide range of commodity polymers. The connection between the experimental and the KG melts is made at the Kuhn scale, i.e., at the crossover from the chemistry-specific small scale to the universal large scale behavior. We expect Kuhn scale-mapped KG models to faithfully represent universal properties dominated by the large scale conformational statistics and dynamics of flexible polymers. In particular, we observe very good agreement between entanglement moduli of our KG models and the experimental moduli of the target polymers. Y1 - 2020 U6 - http://dx.doi.org/10.1021/acs.macromol.9b02428 SN - 1520-5835 VL - 53 IS - 6 SP - 1901 EP - 1916 PB - ACS Publications CY - Washington, DC ER - TY - JOUR A1 - Meyer, Jan A1 - Hentschke, Reinhard A1 - Hager, Jonathan A1 - Hojdis, Nils A1 - Karimi-Varzaneh, Hossein Ali T1 - Molecular Simulation of Viscous Dissipation due to Cyclic Deformation of a Silica–Silica Contact in Filled Rubber JF - Macromolecules Y1 - 2017 U6 - http://dx.doi.org/10.1021/acs.macromol.7b00947 SN - 1520-5835 VL - 50 IS - 17 SP - 6679 EP - 6689 ER - TY - JOUR A1 - Hager, Jonathan A1 - Hentschke, Reinhard A1 - Hojdis, Nils A1 - Karimi-Varzaneh, Hossein Ali T1 - Computer Simulation of Particle–Particle Interaction in a Model Polymer Nanocomposite JF - Macromolecules Y1 - 2015 U6 - http://dx.doi.org/10.1021/acs.macromol.5b01864 SN - 1520-5835 VL - 48 IS - 24 SP - 9039 EP - 9049 ER - TY - JOUR A1 - Waller, Mark P. A1 - Braun, Heiko A1 - Hojdis, Nils A1 - Bühl, Michael T1 - Geometries of Second-Row Transition-Metal Complexes from Density-Functional Theory JF - Journal of Chemical Theory and Computation Y1 - 2007 U6 - http://dx.doi.org/10.1021/ct700178y SN - 1549-9626 VL - 3 IS - 6 SP - 2234 EP - 2242 ER - TY - JOUR A1 - Svaneborg, Carsten A1 - Karimi-Varzaneh, Hossein Ali A1 - Hojdis, Nils A1 - Fleck, Franz A1 - Everaers, Ralf T1 - Kremer-Grest Models for Universal Properties of Specific Common Polymer Species JF - Soft Condensed Matter N2 - The Kremer-Grest (KG) bead-spring model is a near standard in Molecular Dynamic simulations of generic polymer properties. It owes its popularity to its computational efficiency, rather than its ability to represent specific polymer species and conditions. Here we investigate how to adapt the model to match the universal properties of a wide range of chemical polymers species. For this purpose we vary a single parameter originally introduced by Faller and Müller-Plathe, the chain stiffness. Examples include polystyrene, polyethylene, polypropylene, cis-polyisoprene, polydimethylsiloxane, polyethyleneoxide and styrene-butadiene rubber. We do this by matching the number of Kuhn segments per chain and the number of Kuhn segments per cubic Kuhn volume for the polymer species and for the Kremer-Grest model. We also derive mapping relations for converting KG model units back to physical units, in particular we obtain the entanglement time for the KG model as function of stiffness allowing for a time mapping. To test these relations, we generate large equilibrated well entangled polymer melts, and measure the entanglement moduli using a static primitive-path analysis of the entangled melt structure as well as by simulations of step-strain deformation of the model melts. The obtained moduli for our model polymer melts are in good agreement with the experimentally expected moduli. Y1 - 2018 IS - 1606.05008 ER - TY - BOOK A1 - Lauth, Jakob T1 - Physikalische Chemie kompakt KW - Physikalische Chemie Y1 - 2022 SN - 978-3-662-64587-1 U6 - http://dx.doi.org/https://doi.org/10.1007/978-3-662-64588-8 PB - Springer Spektrum CY - Berlin ER - TY - JOUR A1 - Cheenakula, Dheeraja A1 - Hoffstadt, Kevin A1 - Krafft, Simone A1 - Reinecke, Diana A1 - Klose, Holger A1 - Kuperjans, Isabel A1 - Grömping, Markus T1 - Anaerobic digestion of algal–bacterial biomass of an Algal Turf Scrubber system JF - Biomass Conversion and Biorefinery N2 - This study investigated the anaerobic digestion of an algal–bacterial biofilm grown in artificial wastewater in an Algal Turf Scrubber (ATS). The ATS system was located in a greenhouse (50°54′19ʺN, 6°24′55ʺE, Germany) and was exposed to seasonal conditions during the experiment period. The methane (CH4) potential of untreated algal–bacterial biofilm (UAB) and thermally pretreated biofilm (PAB) using different microbial inocula was determined by anaerobic batch fermentation. Methane productivity of UAB differed significantly between microbial inocula of digested wastepaper, a mixture of manure and maize silage, anaerobic sewage sludge, and percolated green waste. UAB using sewage sludge as inoculum showed the highest methane productivity. The share of methane in biogas was dependent on inoculum. Using PAB, a strong positive impact on methane productivity was identified for the digested wastepaper (116.4%) and a mixture of manure and maize silage (107.4%) inocula. By contrast, the methane yield was significantly reduced for the digested anaerobic sewage sludge (50.6%) and percolated green waste (43.5%) inocula. To further evaluate the potential of algal–bacterial biofilm for biogas production in wastewater treatment and biogas plants in a circular bioeconomy, scale-up calculations were conducted. It was found that a 0.116 km2 ATS would be required in an average municipal wastewater treatment plant which can be viewed as problematic in terms of space consumption. However, a substantial amount of energy surplus (4.7–12.5 MWh a−1) can be gained through the addition of algal–bacterial biomass to the anaerobic digester of a municipal wastewater treatment plant. Wastewater treatment and subsequent energy production through algae show dominancy over conventional technologies. KW - Biogas KW - Methane KW - Algal Turf Scrubber KW - Algal–bacterial bioflm KW - Circular bioeconomy Y1 - 2022 U6 - http://dx.doi.org/10.1007/s13399-022-03236-z SN - 2190-6823 N1 - Corresponding author: Dheeraja Cheenakula VL - 13 SP - 15 Seiten PB - Springer CY - Berlin ER - TY - JOUR A1 - Monakhova, Yulia A1 - Diehl, Bernd W.K. T1 - Nuclear magnetic resonance spectroscopy as an elegant tool for a complete quality control of crude heparin material JF - Journal of Pharmaceutical and Biomedical Analysis N2 - Nuclear magnetic resonance (NMR) spectrometric methods for the quantitative analysis of pure heparin in crude heparin is proposed. For quantification, a two-step routine was developed using a USP heparin reference sample for calibration and benzoic acid as an internal standard. The method was successfully validated for its accuracy, reproducibility, and precision. The methodology was used to analyze 20 authentic porcine heparinoid samples having heparin content between 4.25 w/w % and 64.4 w/w %. The characterization of crude heparin products was further extended to a simultaneous analysis of these common ions: sodium, calcium, acetate and chloride. A significant, linear dependence was found between anticoagulant activity and assayed heparin content for thirteen heparinoids samples, for which reference data were available. A Diffused-ordered NMR experiment (DOSY) can be used for qualitative analysis of specific glycosaminoglycans (GAGs) in heparinoid matrices and, potentially, for quantitative prediction of molecular weight of GAGs. NMR spectrometry therefore represents a unique analytical method suitable for the simultaneous quantitative control of organic and inorganic composition of crude heparin samples (especially heparin content) as well as an estimation of other physical and quality parameters (molecular weight, animal origin and activity). KW - NMR spectroscopy KW - Heparin KW - Crude heparin KW - USP KW - Ions Y1 - 2022 U6 - http://dx.doi.org/10.1016/j.jpba.2022.114915 SN - 0731-7085 VL - 219 IS - Article number: 114915 PB - Elsevier CY - New York, NY ER - TY - JOUR A1 - Falkenberg, Fabian A1 - Rahba, Jade A1 - Fischer, David A1 - Bott, Michael A1 - Bongaerts, Johannes A1 - Siegert, Petra T1 - Biochemical characterization of a novel oxidatively stable, halotolerant, and high-alkaline subtilisin from Alkalihalobacillus okhensis Kh10-101T JF - FEBS Open Bio N2 - Halophilic and halotolerant microorganisms represent a promising source of salt-tolerant enzymes suitable for various biotechnological applications where high salt concentrations would otherwise limit enzymatic activity. Considering the current growing enzyme market and the need for more efficient and new biocatalysts, the present study aimed at the characterization of a high-alkaline subtilisin from Alkalihalobacillus okhensis Kh10-101T. The protease gene was cloned and expressed in Bacillus subtilis DB104. The recombinant protease SPAO with 269 amino acids belongs to the subfamily of high-alkaline subtilisins. The biochemical characteristics of purified SPAO were analyzed in comparison with subtilisin Carlsberg, Savinase, and BPN'. SPAO, a monomer with a molecular mass of 27.1 kDa, was active over a wide range of pH 6.0–12.0 and temperature 20–80 °C, optimally at pH 9.0–9.5 and 55 °C. The protease is highly oxidatively stable to hydrogen peroxide and retained 58% of residual activity when incubated at 10 °C with 5% (v/v) H2O2 for 1 h while stimulated at 1% (v/v) H2O2. Furthermore, SPAO was very stable and active at NaCl concentrations up to 5.0 m. This study demonstrates the potential of SPAO for biotechnological applications in the future. KW - Alkalihalobacillus okhensis KW - detergent protease KW - halotolerant protease KW - high-alkaline subtilisin KW - oxidative stable protease Y1 - 2022 U6 - http://dx.doi.org/10.1002/2211-5463.13457 SN - 2211-5463 N1 - Corresponding author: Petra Siegert VL - 12 IS - 10 SP - 1729 EP - 1746 PB - Wiley CY - Hoboken, NJ ER - TY - JOUR A1 - Falkenberg, Fabian A1 - Bott, Michael A1 - Bongaerts, Johannes A1 - Siegert, Petra T1 - Phylogenetic survey of the subtilase family and a data-mining-based search for new subtilisins from Bacillaceae JF - Frontiers in Microbiology N2 - The subtilase family (S8), a member of the clan SB of serine proteases are ubiquitous in all kingdoms of life and fulfil different physiological functions. Subtilases are divided in several groups and especially subtilisins are of interest as they are used in various industrial sectors. Therefore, we searched for new subtilisin sequences of the family Bacillaceae using a data mining approach. The obtained 1,400 sequences were phylogenetically classified in the context of the subtilase family. This required an updated comprehensive overview of the different groups within this family. To fill this gap, we conducted a phylogenetic survey of the S8 family with characterised holotypes derived from the MEROPS database. The analysis revealed the presence of eight previously uncharacterised groups and 13 subgroups within the S8 family. The sequences that emerged from the data mining with the set filter parameters were mainly assigned to the subtilisin subgroups of true subtilisins, high-alkaline subtilisins, and phylogenetically intermediate subtilisins and represent an excellent source for new subtilisin candidates. Y1 - 2022 U6 - http://dx.doi.org/10.3389/fmicb.2022.1017978 SN - 1664-302X VL - 2022 IS - 13 PB - Frontiers CY - Lausanne ER - TY - JOUR A1 - Haeger, Gerrit A1 - Bongaerts, Johannes A1 - Siegert, Petra T1 - A convenient ninhydrin assay in 96-well format for amino acid-releasing enzymes using an air-stable reagent JF - Analytical Biochemistry N2 - An improved and convenient ninhydrin assay for aminoacylase activity measurements was developed using the commercial EZ Nin™ reagent. Alternative reagents from literature were also evaluated and compared. The addition of DMSO to the reagent enhanced the solubility of Ruhemann's purple (RP). Furthermore, we found that the use of a basic, aqueous buffer enhances stability of RP. An acidic protocol for the quantification of lysine was developed by addition of glacial acetic acid. The assay allows for parallel processing in a 96-well format with measurements microtiter plates. Y1 - 2022 U6 - http://dx.doi.org/10.1016/j.ab.2022.114819 SN - 1096-0309 IS - 624 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Welden, Melanie A1 - Severins, Robin A1 - Poghossian, Arshak A1 - Wege, Christina A1 - Bongaerts, Johannes A1 - Siegert, Petra A1 - Keusgen, Michael A1 - Schöning, Michael Josef T1 - Detection of acetoin and diacetyl by a tobacco mosaic virus-assisted field-effect biosensor JF - Chemosensors N2 - Acetoin and diacetyl have a major impact on the flavor of alcoholic beverages such as wine or beer. Therefore, their measurement is important during the fermentation process. Until now, gas chromatographic techniques have typically been applied; however, these require expensive laboratory equipment and trained staff, and do not allow for online monitoring. In this work, a capacitive electrolyte–insulator–semiconductor sensor modified with tobacco mosaic virus (TMV) particles as enzyme nanocarriers for the detection of acetoin and diacetyl is presented. The enzyme acetoin reductase from Alkalihalobacillus clausii DSM 8716ᵀ is immobilized via biotin–streptavidin affinity, binding to the surface of the TMV particles. The TMV-assisted biosensor is electrochemically characterized by means of leakage–current, capacitance–voltage, and constant capacitance measurements. In this paper, the novel biosensor is studied regarding its sensitivity and long-term stability in buffer solution. Moreover, the TMV-assisted capacitive field-effect sensor is applied for the detection of diacetyl for the first time. The measurement of acetoin and diacetyl with the same sensor setup is demonstrated. Finally, the successive detection of acetoin and diacetyl in buffer and in diluted beer is studied by tuning the sensitivity of the biosensor using the pH value of the measurement solution. Y1 - 2022 U6 - http://dx.doi.org/10.3390/chemosensors10060218 SN - 2227-9040 N1 - This article belongs to the Special Issue "Nanostructured Devices for Biochemical Sensing" VL - 10 IS - 6 PB - MDPI CY - Basel ER - TY - JOUR A1 - Ojovan, Michael I. A1 - Steinmetz, Hans-Jürgen T1 - Approaches to Disposal of Nuclear Waste JF - Energies N2 - We present a concise mini overview on the approaches to the disposal of nuclear waste currently used or deployed. The disposal of nuclear waste is the end point of nuclear waste management (NWM) activities and is the emplacement of waste in an appropriate facility without the intention to retrieve it. The IAEA has developed an internationally accepted classification scheme based on the end points of NWM, which is used as guidance. Retention times needed for safe isolation of waste radionuclides are estimated based on the radiotoxicity of nuclear waste. Disposal facilities usually rely on a multi-barrier defence system to isolate the waste from the biosphere, which comprises the natural geological barrier and the engineered barrier system. Disposal facilities could be of a trench type, vaults, tunnels, shafts, boreholes, or mined repositories. A graded approach relates the depth of the disposal facilities’ location with the level of hazard. Disposal practices demonstrate the reliability of nuclear waste disposal with minimal expected impacts on the environment and humans. KW - borehole disposal KW - geological disposal KW - disposal facility KW - retention time KW - nuclear waste Y1 - 2022 U6 - http://dx.doi.org/10.3390/en15207804 SN - 1996-1073 N1 - This article belongs to the Special Issue "Treatment of Radioactive Waste and Sustainability Energy" VL - 15 IS - 20 PB - MDPI CY - Basel ER - TY - JOUR A1 - Zhantlessova, Sirina A1 - Savitskaya, Irina A1 - Kistaubayeva, Aida A1 - Ignatova, Ludmila A1 - Talipova, Aizhan A1 - Pogrebnjak, Alexander A1 - Digel, Ilya T1 - Advanced “Green” prebiotic composite of bacterial cellulose/pullulan based on synthetic biology-powered microbial coculture strategy JF - Polymers N2 - Bacterial cellulose (BC) is a biopolymer produced by different microorganisms, but in biotechnological practice, Komagataeibacter xylinus is used. The micro- and nanofibrillar structure of BC, which forms many different-sized pores, creates prerequisites for the introduction of other polymers into it, including those synthesized by other microorganisms. The study aims to develop a cocultivation system of BC and prebiotic producers to obtain BC-based composite material with prebiotic activity. In this study, pullulan (PUL) was found to stimulate the growth of the probiotic strain Lactobacillus rhamnosus GG better than the other microbial polysaccharides gellan and xanthan. BC/PUL biocomposite with prebiotic properties was obtained by cocultivation of Komagataeibacter xylinus and Aureobasidium pullulans, BC and PUL producers respectively, on molasses medium. The inclusion of PUL in BC is proved gravimetrically by scanning electron microscopy and by Fourier transformed infrared spectroscopy. Cocultivation demonstrated a composite effect on the aggregation and binding of BC fibers, which led to a significant improvement in mechanical properties. The developed approach for “grafting” of prebiotic activity on BC allows preparation of environmentally friendly composites of better quality. KW - coculture KW - pullulan KW - exopolysaccharides KW - prebiotic KW - bacterial cellulose Y1 - 2022 U6 - http://dx.doi.org/10.3390/polym14153224 SN - 2073-4360 N1 - This article belongs to the Special Issue "Cellulose Based Composites" VL - 14 IS - 15 PB - MDPI CY - Basel ER - TY - JOUR A1 - Welden, Melanie A1 - Poghossian, Arshak A1 - Vahidpour, Farnoosh A1 - Wendlandt, Tim A1 - Keusgen, Michael A1 - Wege, Christina A1 - Schöning, Michael Josef T1 - Towards multi-analyte detection with field-effect capacitors modified with tobacco mosaic virus bioparticles as enzyme nanocarriers JF - Biosensors N2 - Utilizing an appropriate enzyme immobilization strategy is crucial for designing enzyme-based biosensors. Plant virus-like particles represent ideal nanoscaffolds for an extremely dense and precise immobilization of enzymes, due to their regular shape, high surface-to-volume ratio and high density of surface binding sites. In the present work, tobacco mosaic virus (TMV) particles were applied for the co-immobilization of penicillinase and urease onto the gate surface of a field-effect electrolyte-insulator-semiconductor capacitor (EISCAP) with a p-Si-SiO₂-Ta₂O₅ layer structure for the sequential detection of penicillin and urea. The TMV-assisted bi-enzyme EISCAP biosensor exhibited a high urea and penicillin sensitivity of 54 and 85 mV/dec, respectively, in the concentration range of 0.1–3 mM. For comparison, the characteristics of single-enzyme EISCAP biosensors modified with TMV particles immobilized with either penicillinase or urease were also investigated. The surface morphology of the TMV-modified Ta₂O₅-gate was analyzed by scanning electron microscopy. Additionally, the bi-enzyme EISCAP was applied to mimic an XOR (Exclusive OR) enzyme logic gate. KW - urease KW - enzyme-logic gate KW - bi-enzyme biosensor KW - capacitive field-effect sensor KW - tobacco mosaic virus (TMV) KW - penicillinase Y1 - 2022 U6 - http://dx.doi.org/10.3390/bios12010043 SN - 2079-6374 N1 - This article belongs to the Special Issue "Biosensors: 10th Anniversary Feature Papers" VL - 12 IS - 1 PB - MDPI CY - Basel ER - TY - JOUR A1 - Welden, Rene A1 - Jablonski, Melanie A1 - Wege, Christina A1 - Keusgen, Michael A1 - Wagner, Patrick Hermann A1 - Wagner, Torsten A1 - Schöning, Michael Josef T1 - Light-Addressable Actuator-Sensor Platform for Monitoring and Manipulation of pH Gradients in Microfluidics: A Case Study with the Enzyme Penicillinase JF - Biosensors N2 - The feasibility of light-addressed detection and manipulation of pH gradients inside an electrochemical microfluidic cell was studied. Local pH changes, induced by a light-addressable electrode (LAE), were detected using a light-addressable potentiometric sensor (LAPS) with different measurement modes representing an actuator-sensor system. Biosensor functionality was examined depending on locally induced pH gradients with the help of the model enzyme penicillinase, which had been immobilized in the microfluidic channel. The surface morphology of the LAE and enzyme-functionalized LAPS was studied by scanning electron microscopy. Furthermore, the penicillin sensitivity of the LAPS inside the microfluidic channel was determined with regard to the analyte’s pH influence on the enzymatic reaction rate. In a final experiment, the LAE-controlled pH inhibition of the enzyme activity was monitored by the LAPS. KW - microfluidics KW - enzyme kinetics KW - actuator-sensor system KW - light-addressable electrode KW - light-addressable potentiometric sensor Y1 - 2021 U6 - http://dx.doi.org/10.3390/bios11060171 SN - 2079-6374 N1 - This article belongs to the Special Issue "Selected Papers from the 1st International Electronic Conference on Biosensors (IECB 2020)" VL - 11 IS - 6 PB - MDPI CY - Basel ER - TY - JOUR A1 - Hafidi, Youssef A1 - El Hatka, Hicham A1 - Schmitz, Dominik A1 - Krauss, Manuel A1 - Pettrak, Jürgen A1 - Biel, Markus A1 - Ittobane, Najim T1 - Sustainable soil additives for water and micronutrient supply: swelling and chelating properties of polyaspartic acid hydrogels utilizing newly developed crosslinkers JF - Gels N2 - Drought and water shortage are serious problems in many arid and semi-arid regions. This problem is getting worse and even continues in temperate climatic regions due to climate change. To address this problem, the use of biodegradable hydrogels is increasingly important for the application as water-retaining additives in soil. Furthermore, efficient (micro-)nutrient supply can be provided by the use of tailored hydrogels. Biodegradable polyaspartic acid (PASP) hydrogels with different available (1,6-hexamethylene diamine (HMD) and L-lysine (LYS)) and newly developed crosslinkers based on diesters of glycine (GLY) and (di-)ethylene glycol (DEG and EG, respectively) were synthesized and characterized using Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM) and regarding their swelling properties (kinetic, absorbency under load (AUL)) as well as biodegradability of PASP hydrogel. Copper (II) and zinc (II), respectively, were loaded as micronutrients in two different approaches: in situ with crosslinking and subsequent loading of prepared hydrogels. The results showed successful syntheses of di-glycine-ester-based crosslinkers. Hydrogels with good water-absorbing properties were formed. Moreover, the developed crosslinking agents in combination with the specific reaction conditions resulted in higher water absorbency with increased crosslinker content used in synthesis (10% vs. 20%). The prepared hydrogels are candidates for water-storing soil additives due to the biodegradability of PASP, which is shown in an exemple. The incorporation of Cu(II) and Zn(II) ions can provide these micronutrients for plant growth. KW - micronutrients KW - swelling properties KW - biodegradable polymers KW - hydrogels KW - superabsorbent polymers KW - glycine KW - polyaspartic acid Y1 - 2024 U6 - http://dx.doi.org/10.3390/gels10030170 SN - 2310-2861 VL - 10 IS - 3 SP - Artikel 170 PB - MDPI CY - Basel ER - TY - RPRT A1 - Tippkötter, Nils A1 - Wagner, Sebastian T1 - Biomimetischer Klebstoff aus ligninhaltigen Pflanzenresten (Teilvorhaben 1 und 2) : Schlussbericht zum Vorhaben : Laufzeit: 01.01.2016 bis 31.03.2019 Y1 - 2019 U6 - http://dx.doi.org/10.2314/KXP:169732777X N1 - Förderkennzeichen BMEL 22030514 - 22004615 PB - FH Aachen CY - Jülich ER - TY - JOUR A1 - Capitain, Charlotte A1 - Wagner, Sebastian A1 - Hummel, Joana A1 - Tippkötter, Nils T1 - Investigation of C–N Formation Between Catechols and Chitosan for the Formation of a Strong, Novel Adhesive Mimicking Mussel Adhesion JF - Waste and Biomass Valorization Y1 - 2021 U6 - http://dx.doi.org/10.1007/s12649-020-01110-5 SN - 1877-265X N1 - Corresponding author: Nils Tippkötter VL - 12 SP - 1761 EP - 1779 PB - Springer Nature CY - Cham ER - TY - CHAP A1 - Wulfhorst, Helene A1 - Merseburg, Johannes A1 - Tippkötter, Nils T1 - Analyse von Lignocellulose mittels dynamischer Differenzkalorimetrie und Infrarot – Spektrometrie T2 - 12. Dresdner Sensor-Symposium 2015 2015-12-07 - 2015-12-09 Y1 - 2015 SN - 978-3-9813484-9-1 U6 - http://dx.doi.org/10.5162/12dss2015/P6.2 SP - 210 EP - 215 ER - TY - PAT A1 - Stadtmüller, Ralf A1 - Tippkötter, Nils A1 - Ulber, Roland T1 - Method for production of single-stranded macronucleotides N2 - The invention relates to a method for production of single-stranded macronucleotides by amplifying and ligating an extended monomeric single-stranded target nucleic acid sequence (targetss) into a repetitive cluster of double-stranded target nucleic acid sequences (targetds), and subsequently cloning the construct into a vector (aptagene vector). The aptagene vector is transformed into host cells for replication of the aptagene and isolated in order to optain single-stranded target sequences (targetss). The invention also relates to single-stranded nucleic acids, produced by a method of the invention. Y1 - 2015 N1 - Patent auch unter EP2774996, EP2774996, US2017145460 und US9944966 veröffentlicht. ER - TY - PAT A1 - Huschyar, Al-Kaidy A1 - Tippkötter, Nils A1 - Ulber, Roland T1 - System und Verfahren zur Durchführung von chemischen, biologischen oder physikalischen Reaktionen Y1 - 2015 N1 - Patent EP2821134A1 ER - TY - PAT A1 - Al-Kaidy, Huschyar A1 - Tippkötter, Nils A1 - Ulber, Roland T1 - Vorrichtung und Verfahren zur Bestimmung des Kontaktwinkels eines Flüssigkörpers mit einer Festkörperoberfläche N2 - Die vorliegende Erfindung betrifft eine Vorrichtung und ein Verfahren zur Bestimmung des Kontaktwinkels eines flüssigen oder mit Flüssigkeit gefüllten Körpers. Dieser besteht aus einem Träger (1) und einer damit verbundenen, in einem Winkelbereich von mehr als 0 ° bis maximal 90 ° neigbaren Ebene (8) mit einer darin ausgebildeten Abrollbahn (9) für den flüssigen oder mit Flüssigkeit gefüllten Körper. An der Ebene (8) sind mehrere Sensoren (11,12) zur Erfassung der Rolldauer des Körpers entlang der Rollstrecke angeordnet. Erfindungsgemäß ist vorgesehen, dass die Einstellung des Neigungswinkels der Ebene (8) über ein Winkelmessgerät (10) erfolgt, wodurch ein Abrollwinkel erfassbar ist, bei dem der Körper in Bewegung gerät. Aus der Rolldauer, der Rollstrecke und dem Abrollwinkel wird der Kontaktwinkel des Körpers ermittelt. Y1 - 2016 N1 - Patent auch unter DE102014000419 und WO2015107159 veröffentlicht. ER - TY - JOUR A1 - Monakhova, Yulia A1 - Diehl, Bernd W.K. T1 - Novel approach of qNMR workflow by standardization using 2H integral: Application to any intrinsic calibration standard JF - Talanta N2 - Quantitative nuclear magnetic resonance (qNMR) is routinely performed by the internal or external standardization. The manuscript describes a simple alternative to these common workflows by using NMR signal of another active nuclei of calibration compound. For example, for any arbitrary compound quantification by NMR can be based on the use of an indirect concentration referencing that relies on a solvent having both 1H and 2H signals. To perform high-quality quantification, the deuteration level of the utilized deuterated solvent has to be estimated. In this contribution the new method was applied to the determination of deuteration levels in different deuterated solvents (MeOD, ACN, CDCl3, acetone, benzene, DMSO-d6). Isopropanol-d6, which contains a defined number of deuterons and protons, was used for standardization. Validation characteristics (precision, accuracy, robustness) were calculated and the results showed that the method can be used in routine practice. Uncertainty budget was also evaluated. In general, this novel approach, using standardization by 2H integral, benefits from reduced sample preparation steps and uncertainties, and can be applied in different application areas (purity determination, forensics, pharmaceutical analysis, etc.). KW - qNMR KW - Deuterium NMR KW - Deuterated solvents KW - Standardization Y1 - 2021 SN - 0039-9140 U6 - http://dx.doi.org/10.1016/j.talanta.2020.121504 VL - 222 IS - Article number: 121504 PB - Elsevier ER - TY - JOUR A1 - Burmistrova, Natalia A. A1 - Soboleva, Polina M. A1 - Monakhova, Yulia T1 - Is infrared spectroscopy combined with multivariate analysis a promising tool for heparin authentication? JF - Journal of Pharmaceutical and Biomedical Analysis N2 - The investigation of the possibility to determine various characteristics of powder heparin (n = 115) was carried out with infrared spectroscopy. The evaluation of heparin samples included several parameters such as purity grade, distributing company, animal source as well as heparin species (i.e. Na-heparin, Ca-heparin, and heparinoids). Multivariate analysis using principal component analysis (PCA), soft independent modelling of class analogy (SIMCA), and partial least squares – discriminant analysis (PLS-DA) were applied for the modelling of spectral data. Different pre-processing methods were applied to IR spectral data; multiplicative scatter correction (MSC) was chosen as the most relevant. Obtained results were confirmed by nuclear magnetic resonance (NMR) spectroscopy. Good predictive ability of this approach demonstrates the potential of IR spectroscopy and chemometrics for screening of heparin quality. This approach, however, is designed as a screening tool and is not considered as a replacement for either of the methods required by USP and FDA. KW - IR spectroscopy KW - Heparin KW - Authenticity KW - Principal component analysis KW - Soft independent modeling of class analogy Y1 - 2021 SN - 0731-7085 U6 - http://dx.doi.org/10.1016/j.jpba.2020.113811 VL - 194 IS - Article number: 113811 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Monakhova, Yulia A1 - Diehl, Bernd W. K. T1 - Simplification of NMR Workflows by Standardization Using 2H Integral of Deuterated Solvent as Applied to Aloe vera Preparations JF - Applied Magnetic Resonance N2 - In this study, a recently proposed NMR standardization approach by 2H integral of deuterated solvent for quantitative multicomponent analysis of complex mixtures is presented. As a proof of principle, the existing NMR routine for the analysis of Aloe vera products was modified. Instead of using absolute integrals of targeted compounds and internal standard (nicotinamide) from 1H-NMR spectra, quantification was performed based on the ratio of a particular 1H-NMR compound integral and 2H-NMR signal of deuterated solvent D2O. Validation characteristics (linearity, repeatability, accuracy) were evaluated and the results showed that the method has the same precision as internal standardization in case of multicomponent screening. Moreover, a dehydration process by freeze drying is not necessary for the new routine. Now, our NMR profiling of A. vera products needs only limited sample preparation and data processing. The new standardization methodology provides an appealing alternative for multicomponent NMR screening. In general, this novel approach, using standardization by 2H integral, benefits from reduced sample preparation steps and uncertainties, and is recommended in different application areas (purity determination, forensics, pharmaceutical analysis, etc.). Y1 - 2021 U6 - http://dx.doi.org/10.1007/s00723-021-01393-4 SN - 1613-7507 VL - 52 IS - 11 SP - 1591 EP - 1600 PB - Springer CY - Cham ER - TY - JOUR A1 - Burger, René A1 - Rumpf, Jessica A1 - Do, Xuan Tung A1 - Monakhova, Yulia A1 - Diehl, Bernd W. K. A1 - Rehahn, Matthias A1 - Schulze, Margit T1 - Is NMR combined with multivariate regression applicable for the molecular weight determination of randomly cross-linked polymers such as lignin? JF - ACS Omega N2 - The molecular weight properties of lignins are one of the key elements that need to be analyzed for a successful industrial application of these promising biopolymers. In this study, the use of 1H NMR as well as diffusion-ordered spectroscopy (DOSY NMR), combined with multivariate regression methods, was investigated for the determination of the molecular weight (Mw and Mn) and the polydispersity of organosolv lignins (n = 53, Miscanthus x giganteus, Paulownia tomentosa, and Silphium perfoliatum). The suitability of the models was demonstrated by cross validation (CV) as well as by an independent validation set of samples from different biomass origins (beech wood and wheat straw). CV errors of ca. 7–9 and 14–16% were achieved for all parameters with the models from the 1H NMR spectra and the DOSY NMR data, respectively. The prediction errors for the validation samples were in a similar range for the partial least squares model from the 1H NMR data and for a multiple linear regression using the DOSY NMR data. The results indicate the usefulness of NMR measurements combined with multivariate regression methods as a potential alternative to more time-consuming methods such as gel permeation chromatography. Y1 - 2021 U6 - http://dx.doi.org/10.1021/acsomega.1c03574 SN - 2470-1343 VL - 6 IS - 44 SP - 29516 EP - 29524 PB - ACS Publications CY - Washington, DC ER - TY - JOUR A1 - Lindner, Simon A1 - Burger, René A1 - Rutledge, Douglas N. A1 - Do, Xuan Tung A1 - Rumpf, Jessica A1 - Diehl, Bernd W. K. A1 - Schulze, Margit A1 - Monakhova, Yulia T1 - Is the calibration transfer of multivariate calibration models between high- and low-field NMR instruments possible? A case study of lignin molecular weight JF - Analytical chemistry N2 - Although several successful applications of benchtop nuclear magnetic resonance (NMR) spectroscopy in quantitative mixture analysis exist, the possibility of calibration transfer remains mostly unexplored, especially between high- and low-field NMR. This study investigates for the first time the calibration transfer of partial least squares regressions [weight average molecular weight (Mw) of lignin] between high-field (600 MHz) NMR and benchtop NMR devices (43 and 60 MHz). For the transfer, piecewise direct standardization, calibration transfer based on canonical correlation analysis, and transfer via the extreme learning machine auto-encoder method are employed. Despite the immense resolution difference between high-field and low-field NMR instruments, the results demonstrate that the calibration transfer from high- to low-field is feasible in the case of a physical property, namely, the molecular weight, achieving validation errors close to the original calibration (down to only 1.2 times higher root mean square errors). These results introduce new perspectives for applications of benchtop NMR, in which existing calibrations from expensive high-field instruments can be transferred to cheaper benchtop instruments to economize. Y1 - 2022 SN - 1520-6882 U6 - http://dx.doi.org/10.1021/acs.analchem.1c05125 VL - 94 IS - 9 SP - 3997 EP - 4004 PB - ACS Publications CY - Washington, DC ER - TY - JOUR A1 - Monakhova, Yulia A1 - Diehl, Bernd W.K. T1 - Multinuclear NMR screening of pharmaceuticals using standardization by 2H integral of a deuterated solvent JF - Journal of Pharmaceutical and Biomedical Analysis N2 - NMR standardization approach that uses the 2H integral of deuterated solvent for quantitative multinuclear analysis of pharmaceuticals is described. As a proof of principle, the existing NMR procedure for the analysis of heparin products according to US Pharmacopeia monograph is extended to the determination of Na+ and Cl- content in this matrix. Quantification is performed based on the ratio of a 23Na (35Cl) NMR integral and 2H NMR signal of deuterated solvent, D2O, acquired using the specific spectrometer hardware. As an alternative, the possibility of 133Cs standardization using the addition of Cs2CO3 stock solution is shown. Validation characteristics (linearity, repeatability, sensitivity) are evaluated. A holistic NMR profiling of heparin products can now also be used for the quantitative determination of inorganic compounds in a single analytical run using a single sample. In general, the new standardization methodology provides an appealing alternative for the NMR screening of inorganic and organic components in pharmaceutical products. KW - NMR spectroscopy KW - Inorganic ions KW - Heparin KW - Standardization Y1 - 2022 SN - 0731-7085 U6 - http://dx.doi.org/10.1016/j.jpba.2021.114530 VL - 209 IS - Article number: 114530 PB - Elsevier ER - TY - JOUR A1 - Burger, René A1 - Lindner, Simon A1 - Rumpf, Jessica A1 - Do, Xuan Tung A1 - Diehl, Bernd W.K. A1 - Rehahn, Matthias A1 - Monakhova, Yulia A1 - Schulze, Margit T1 - Benchtop versus high field NMR: Comparable performance found for the molecular weight determination of lignin JF - Journal of Pharmaceutical and Biomedical Analysis N2 - Lignin is a promising renewable biopolymer being investigated worldwide as an environmentally benign substitute of fossil-based aromatic compounds, e.g. for the use as an excipient with antioxidant and antimicrobial properties in drug delivery or even as active compound. For its successful implementation into process streams, a quick, easy, and reliable method is needed for its molecular weight determination. Here we present a method using 1H spectra of benchtop as well as conventional NMR systems in combination with multivariate data analysis, to determine lignin’s molecular weight (Mw and Mn) and polydispersity index (PDI). A set of 36 organosolv lignin samples (from Miscanthus x giganteus, Paulownia tomentosa and Silphium perfoliatum) was used for the calibration and cross validation, and 17 samples were used as external validation set. Validation errors between 5.6% and 12.9% were achieved for all parameters on all NMR devices (43, 60, 500 and 600 MHz). Surprisingly, no significant difference in the performance of the benchtop and high-field devices was found. This facilitates the application of this method for determining lignin’s molecular weight in an industrial environment because of the low maintenance expenditure, small footprint, ruggedness, and low cost of permanent magnet benchtop NMR systems. KW - NMR KW - PLS-regression KW - Molecular weight determination KW - Chemometrics KW - Biomass Y1 - 2022 SN - 0731-7085 U6 - http://dx.doi.org/10.1016/j.jpba.2022.114649 VL - 212 IS - Article number: 114649 PB - Elsevier CY - New York, NY ER - TY - JOUR A1 - Monakhova, Yulia A1 - Soboleva, Polina M. A1 - Fedotova, Elena S. A1 - Musina, Kristina T. A1 - Burmistrova, Natalia A. T1 - Quantum chemical calculations of IR spectra of heparin disaccharide subunits JF - Computational and Theoretical Chemistry N2 - Heparin is a natural polysaccharide, which plays essential role in many biological processes. Alterations in building blocks can modify biological roles of commercial heparin products, due to significant changes in the conformation of the polymer chain. The variability structure of heparin leads to difficulty in quality control using different analytical methods, including infrared (IR) spectroscopy. In this paper molecular modelling of heparin disaccharide subunits was performed using quantum chemistry. The structural and spectral parameters of these disaccharides have been calculated using RHF/6-311G. In addition, over-sulphated chondroitin sulphate disaccharide was studied as one of the most widespread contaminants of heparin. Calculated IR spectra were analyzed with respect to specific structure parameters. IR spectroscopic fingerprint was found to be sensitive to substitution pattern of disaccharide subunits. Vibrational assignments of calculated spectra were correlated with experimental IR spectral bands of native heparin. Chemometrics was used to perform multivariate analysis of simulated spectral data. KW - IR spectroscopy KW - Chemometrics KW - Quantum chemistry KW - Molecular modelling KW - Quality control Y1 - 2022 SN - 2210-271X U6 - http://dx.doi.org/10.1016/j.comptc.2022.113891 VL - 1217 IS - Article number: 113891 PB - Elsevier CY - New York, NY ER - TY - JOUR A1 - Huck, Christina A1 - Schiffels, Johannes A1 - Herrera, Cony N. A1 - Schelden, Maximilian A1 - Selmer, Thorsten A1 - Poghossian, Arshak A1 - Baumann, Marcus A1 - Wagner, Patrick A1 - Schöning, Michael Josef T1 - Metabolic responses of Escherichia coli upon glucose pulses captured by a capacitive field-effect sensor JF - Physica Status Solidi (A) N2 - Living cells are complex biological systems transforming metabolites taken up from the surrounding medium. Monitoring the responses of such cells to certain substrate concentrations is a challenging task and offers possibilities to gain insight into the vitality of a community influenced by the growth environment. Cell-based sensors represent a promising platform for monitoring the metabolic activity and thus, the “welfare” of relevant organisms. In the present study, metabolic responses of the model bacterium Escherichia coli in suspension, layered onto a capacitive field-effect structure, were examined to pulses of glucose in the concentration range between 0.05 and 2 mM. It was found that acidification of the surrounding medium takes place immediately after glucose addition and follows Michaelis–Menten kinetic behavior as a function of the glucose concentration. In future, the presented setup can, therefore, be used to study substrate specificities on the enzymatic level and may as well be used to perform investigations of more complex metabolic responses. Conclusions and perspectives highlighting this system are discussed. Y1 - 2013 U6 - http://dx.doi.org/10.1002/pssa.201200900 SN - 0031-8965 VL - 210 IS - 5 SP - 926 EP - 931 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Bäcker, Matthias A1 - Rakowski, D. A1 - Poghossian, Arshak A1 - Biselli, Manfred A1 - Wagner, Patrick A1 - Schöning, Michael Josef T1 - Chip-based amperometric enzyme sensor system for monitoring of bioprocesses by flow-injection analysis JF - Journal of Biotechnology N2 - A microfluidic chip integrating amperometric enzyme sensors for the detection of glucose, glutamate and glutamine in cell-culture fermentation processes has been developed. The enzymes glucose oxidase, glutamate oxidase and glutaminase were immobilized by means of cross-linking with glutaraldehyde on platinum thin-film electrodes integrated within a microfluidic channel. The biosensor chip was coupled to a flow-injection analysis system for electrochemical characterization of the sensors. The sensors have been characterized in terms of sensitivity, linear working range and detection limit. The sensitivity evaluated from the respective peak areas was 1.47, 3.68 and 0.28 μAs/mM for the glucose, glutamate and glutamine sensor, respectively. The calibration curves were linear up to a concentration of 20 mM glucose and glutamine and up to 10 mM for glutamate. The lower detection limit amounted to be 0.05 mM for the glucose and glutamate sensor, respectively, and 0.1 mM for the glutamine sensor. Experiments in cell-culture medium have demonstrated a good correlation between the glutamate, glutamine and glucose concentrations measured with the chip-based biosensors in a differential-mode and the commercially available instrumentation. The obtained results demonstrate the feasibility of the realized microfluidic biosensor chip for monitoring of bioprocesses. Y1 - 2013 U6 - http://dx.doi.org/10.1016/j.jbiotec.2012.03.014 SN - 0168-1656 VL - 163 IS - 4 SP - 371 EP - 376 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Ross, Jillian A1 - Plummer, Simon M. A1 - Rode, Anja A1 - Scheer, Nico A1 - Bower, Conrad C. A1 - Vogel, Ortwin A1 - Henderson, Colin J. A1 - Wolf, C. Roland A1 - Elcombe, Clifford R. T1 - Human constitutive androstane receptor (CAR) and pregnane X receptor (PXR) support the hypertrophic but not the hyperplastic response to the murine nongenotoxic hepatocarcinogens phenobarbital and chlordane in vivo JF - Toxicological Sciences N2 - Mouse nongenotoxic hepatocarcinogens phenobarbital (PB) and chlordane induce hepatomegaly characterized by hypertrophy and hyperplasia. Increased cell proliferation is implicated in the mechanism of tumor induction. The relevance of these tumors to human health is unclear. The xenoreceptors, constitutive androstane receptors (CARs), and pregnane X receptor (PXR) play key roles in these processes. Novel “humanized” and knockout models for both receptors were developed to investigate potential species differences in hepatomegaly. The effects of PB (80 mg/kg/4 days) and chlordane (10 mg/kg/4 days) were investigated in double humanized PXR and CAR (huPXR/huCAR), double knockout PXR and CAR (PXRKO/CARKO), and wild-type (WT) C57BL/6J mice. In WT mice, both compounds caused increased liver weight, hepatocellular hypertrophy, and cell proliferation. Both compounds caused alterations to a number of cell cycle genes consistent with induction of cell proliferation in WT mice. However, these gene expression changes did not occur in PXRKO/CARKO or huPXR/huCAR mice. Liver hypertrophy without hyperplasia was demonstrated in the huPXR/huCAR animals in response to both compounds. Induction of the CAR and PXR target genes, Cyp2b10 and Cyp3a11, was observed in both WT and huPXR/huCAR mouse lines following treatment with PB or chlordane. In the PXRKO/CARKO mice, neither liver growth nor induction of Cyp2b10 and Cyp3a11 was seen following PB or chlordane treatment, indicating that these effects are CAR/PXR dependent. These data suggest that the human receptors are able to support the chemically induced hypertrophic responses but not the hyperplastic (cell proliferation) responses. At this time, we cannot be certain that hCAR and hPXR when expressed in the mouse can function exactly as the genes do when they are expressed in human cells. However, all parameters investigated to date suggest that much of their functionality is maintained. Y1 - 2010 U6 - http://dx.doi.org/10.1093/toxsci/kfq118 SN - 1096-0929 VL - 116 IS - 2 SP - 452 EP - 466 PB - Oxford University Press CY - Oxford ER - TY - JOUR A1 - Scheer, Nico A1 - Ross, Jillian A1 - Kapelyukh, Yury A1 - Rode, Anja A1 - Wolf, C. Roland T1 - In vivo responses of the human and murine pregnane X receptor to dexamethasone in mice JF - Drug Metabolism and Disposition N2 - Dexamethasone (DEX) is a potent and widely used anti-inflammatory and immunosuppressant glucocorticoid. It can bind and activate the pregnane X receptor (PXR), which plays a critical role as xenobiotic sensor in mammals to induce the expression of many enzymes, including cytochromes P450 in the CYP3A family. This induction results in its own metabolism. We have used a series of transgenic mouse lines, including a novel, improved humanized PXR line, to compare the induction profile of PXR-regulated drug-metabolizing enzymes after DEX administration, as well as looking at hepatic responses to rifampicin (RIF). The new humanized PXR model has uncovered further intriguing differences between the human and mouse receptors in that RIF only induced Cyp2b10 in the new humanized model. DEX was found to be a much more potent inducer of Cyp3a proteins in wild-type mice than in mice humanized for PXR. To assess whether PXR is involved in the detoxification of DEX in the liver, we analyzed the consequences of high doses of the glucocorticoid on hepatotoxicity on different PXR genetic backgrounds. We also studied these effects in an additional mouse model in which functional mouse Cyp3a genes have been deleted. These strains exhibited different sensitivities to DEX, indicating a protective role of the PXR and CYP3A proteins against the hepatotoxicity of this compound. Y1 - 2010 U6 - http://dx.doi.org/10.1124/dmd.109.031872 SN - 1521-009X VL - 38 IS - 7 SP - 1046 EP - 1053 PB - ASPET CY - Bethesda ER - TY - JOUR A1 - Schmidt, Aaron C. A1 - Turgut, Hatice A1 - Le, Dao A1 - Beloqui, Ana A1 - Delaittre, Guillaume T1 - Making the best of it: nitroxide-mediated polymerization of methacrylates via the copolymerization approach with functional styrenics JF - Polymer Chemistry N2 - The SG1-mediated solution polymerization of methyl methacrylate (MMA) and oligo(ethylene glycol) methacrylate (OEGMA, Mₙ = 300 g mol⁻¹) in the presence of a small amount of functional/reactive styrenic comonomer is investigated. Moieties such as pentafluorophenyl ester, triphenylphosphine, azide, pentafluorophenyl, halide, and pyridine are considered. A comonomer fraction as low as 5 mol% typically results in a controlled/living behavior, at least up to 50% conversion. Chain extensions with styrene for both systems were successfully performed. Variation of physical properties such as refractive index (for MMA) and phase transition temperature (for OEGMA) were evaluated by comparing to 100% pure homopolymers. The introduction of an activated ester styrene derivative in the polymerization of OEGMA allows for the synthesis of reactive and hydrophilic polymer brushes with defined thickness. Finally, using the example of pentafluorostyrene as controlling comonomer, it is demonstrated that functional PMMA-b-PS are able to maintain a phase separation ability, as evidenced by the formation of nanostructured thin films. Y1 - 2020 U6 - http://dx.doi.org/10.1039/C9PY01458F VL - 11 IS - 2 SP - 593 EP - 604 PB - Royal Society of Chemistry (RSC) CY - Cambridge ER - TY - JOUR A1 - Capitain, Charlotte A1 - Ross-Jones, Jesse A1 - Möhring, Sophie A1 - Tippkötter, Nils T1 - Differential scanning calorimetry for quantification of polymer biodegradability in compost JF - International Biodeterioration & Biodegradation N2 - The objective of this study is the establishment of a differential scanning calorimetry (DSC) based method for online analysis of the biodegradation of polymers in complex environments. Structural changes during biodegradation, such as an increase in brittleness or crystallinity, can be detected by carefully observing characteristic changes in DSC profiles. Until now, DSC profiles have not been used to draw quantitative conclusions about biodegradation. A new method is presented for quantifying the biodegradation using DSC data, whereby the results were validated using two reference methods. The proposed method is applied to evaluate the biodegradation of three polymeric biomaterials: polyhydroxybutyrate (PHB), cellulose acetate (CA) and Organosolv lignin. The method is suitable for the precise quantification of the biodegradability of PHB. For CA and lignin, conclusions regarding their biodegradation can be drawn with lower resolutions. The proposed method is also able to quantify the biodegradation of blends or composite materials, which differentiates it from commonly used degradation detection methods. Y1 - 2020 U6 - http://dx.doi.org/10.1016/j.ibiod.2020.104914 SN - 0964-8305 VL - 149 SP - In Press, Article number 104914 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Muschallik, Lukas A1 - Molinnus, Denise A1 - Jablonski, Melanie A1 - Kipp, Carina Ronja A1 - Bongaerts, Johannes A1 - Pohl, Martina A1 - Wagner, Torsten A1 - Schöning, Michael Josef A1 - Selmer, Thorsten A1 - Siegert, Petra T1 - Synthesis of α-hydroxy ketones and vicinal (R, R)-diols by Bacillus clausii DSM 8716ᵀ butanediol dehydrogenase JF - RSC Advances N2 - α-hydroxy ketones (HK) and 1,2-diols are important building blocks for fine chemical synthesis. Here, we describe the R-selective 2,3-butanediol dehydrogenase from B. clausii DSM 8716ᵀ (BcBDH) that belongs to the metal-dependent medium chain dehydrogenases/reductases family (MDR) and catalyzes the selective asymmetric reduction of prochiral 1,2-diketones to the corresponding HK and, in some cases, the reduction of the same to the corresponding 1,2-diols. Aliphatic diketones, like 2,3-pentanedione, 2,3-hexanedione, 5-methyl-2,3-hexanedione, 3,4-hexanedione and 2,3-heptanedione are well transformed. In addition, surprisingly alkyl phenyl dicarbonyls, like 2-hydroxy-1-phenylpropan-1-one and phenylglyoxal are accepted, whereas their derivatives with two phenyl groups are not substrates. Supplementation of Mn²⁺ (1 mM) increases BcBDH's activity in biotransformations. Furthermore, the biocatalytic reduction of 5-methyl-2,3-hexanedione to mainly 5-methyl-3-hydroxy-2-hexanone with only small amounts of 5-methyl-2-hydroxy-3-hexanone within an enzyme membrane reactor is demonstrated. Y1 - 2020 U6 - http://dx.doi.org/10.1039/D0RA02066D SN - 2046-2069 VL - 10 SP - 12206 EP - 12216 PB - Royal Society of Chemistry (RSC) CY - Cambridge ER - TY - JOUR A1 - Wardoyo, Arinto Y.P. A1 - Noor, Johan A.E. A1 - Elbers, Gereon A1 - Schmitz, Sandra A1 - Flaig, Sascha T. A1 - Budianto, Arif T1 - Characterizing volcanic ash elements from the 2015 eruptions of bromo and raung volcanoes, Indonesia JF - Polish Journal of Environmental Studies N2 - The volcanic eruptions of Mt. Bromo and Mt. Raung in East Java, Indonesia, in 2015 perturbed volcanic materials and affected surface-layer air quality at surrounding locations. During the episodes, the volcanic ash from the eruptions influenced visibility, traffic accidents, flight schedules, and human health. In this research, the volcanic ash particles were collected and characterized by relying on the detail of physical observation. We performed an assessment of the volcanic ash elements to characterize the volcanic ash using two different methods which are aqua regia extracts followed by MP-AES and XRF laboratory test of bulk samples. The analysis results showed that the volcanic ash was mixed of many materials, such as Al, Si, P, K, Ca, Ti, V, Cr, Mn, Fe, Ni, and others. Fe, Si, Ca, and Al were found as the major elements, while the others were the trace elements Ba, Cr, Cu, Mn, P, Mn, Ni, Zn, Sb, Sr, and V with the minor concentrations. XRF analyses showed that Fe dominated the elements of the volcanic ash. The XRF analysis showed that Fe was at 35.40% in Bromo and 43.00% in Raung of the detected elements in bulk material. The results of aqua regia extracts analyzed by MP-AES were 1.80% and 1.70% of Fe element for Bromo and Raung volcanoes, respectively. Y1 - 2020 U6 - http://dx.doi.org/10.15244/pjoes/99101 SN - 2083-5906 VL - 29 IS - 2 SP - 1899 EP - 1907 PB - HARD CY - Olsztyn ER - TY - JOUR A1 - Lowis, Carsten A1 - Ferguson, Simon A1 - Paulßen, Elisabeth A1 - Hoehr, Cornelia T1 - Improved Sc-44 production in a siphon-style liquid target on a medical cyclotron JF - Applied Radiation and Isotopes Y1 - 2021 U6 - http://dx.doi.org/10.1016/j.apradiso.2021.109675 SN - 0969-8043 VL - 172 IS - Art. 109675 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Bergs, Michel A1 - Monakhova, Yulia A1 - Diehl, Bernd W. A1 - Konow, Christopher A1 - Völkering, Georg A1 - Pude, Ralf A1 - Schulze, Margit T1 - Lignins isolated via catalyst-free organosolv pulping from Miscanthus x giganteus, M. sinensis, M. robustus and M. nagara: a comparative study JF - Molecules N2 - As a low-input crop, Miscanthus offers numerous advantages that, in addition to agricultural applications, permits its exploitation for energy, fuel, and material production. Depending on the Miscanthus genotype, season, and harvest time as well as plant component (leaf versus stem), correlations between structure and properties of the corresponding isolated lignins differ. Here, a comparative study is presented between lignins isolated from M. x giganteus, M. sinensis, M. robustus and M. nagara using a catalyst-free organosolv pulping process. The lignins from different plant constituents are also compared regarding their similarities and differences regarding monolignol ratio and important linkages. Results showed that the plant genotype has the weakest influence on monolignol content and interunit linkages. In contrast, structural differences are more significant among lignins of different harvest time and/or season. Analyses were performed using fast and simple methods such as nuclear magnetic resonance (NMR) spectroscopy. Data was assigned to four different linkages (A: β-O-4 linkage, B: phenylcoumaran, C: resinol, D: β-unsaturated ester). In conclusion, A content is particularly high in leaf-derived lignins at just under 70% and significantly lower in stem and mixture lignins at around 60% and almost 65%. The second most common linkage pattern is D in all isolated lignins, the proportion of which is also strongly dependent on the crop portion. Both stem and mixture lignins, have a relatively high share of approximately 20% or more (maximum is M. sinensis Sin2 with over 30%). In the leaf-derived lignins, the proportions are significantly lower on average. Stem samples should be chosen if the highest possible lignin content is desired, specifically from the M. x giganteus genotype, which revealed lignin contents up to 27%. Due to the better frost resistance and higher stem stability, M. nagara offers some advantages compared to M. x giganteus. Miscanthus crops are shown to be very attractive lignocellulose feedstock (LCF) for second generation biorefineries and lignin generation in Europe. Y1 - 2021 U6 - http://dx.doi.org/10.3390/molecules26040842 SN - 1420-3049 N1 - Special Issue Lignin – A Natural Resource with Huge Potential https://www.mdpi.com/journal/molecules/special_issues/lignin_natural VL - 26 IS - 4 PB - MDPI CY - Basel ER - TY - JOUR A1 - Eckert, Alexander A1 - Abbasi, Mozhdeh A1 - Mang, Thomas A1 - Saalwächter, Kay A1 - Walther, Andreas T1 - Structure, Mechanical Properties, and Dynamics of Polyethylenoxide/Nanoclay Nacre-Mimetic Nanocomposites JF - Macromolecules N2 - Nacre-mimetic nanocomposites based on high fractions of synthetic high-aspect-ratio nanoclays in combination with polymers are continuously pushing boundaries for advanced material properties, such as high barrier against oxygen, extraordinary mechanical behavior, fire shielding, and glass-like transparency. Additionally, they provide interesting model systems to study polymers under nanoconfinement due to the well-defined layered nanocomposite arrangement. Although the general behavior in terms of forming such layered nanocomposite materials using evaporative self-assembly and controlling the nanoclay gallery spacing by the nanoclay/polymer ratio is understood, some combinations of polymer matrices and nanoclay reinforcement do not comply with the established models. Here, we demonstrate a thorough characterization and analysis of such an unusual polymer/nanoclay pair that falls outside of the general behavior. Poly(ethylene oxide) (PEO) and sodium fluorohectorite form nacre-mimetic, lamellar nanocomposites that are completely transparent and show high mechanical stiffness and high gas barrier, but there is only limited expansion of the nanoclay gallery spacing when adding increasing amounts of polymer. This behavior is maintained for molecular weights of PEO varied over four orders of magnitude and can be traced back to depletion forces. By careful investigation via X-ray diffraction and proton low-resolution solid-state NMR, we are able to quantify the amount of mobile and immobilized polymer species in between the nanoclay galleries and around proposed tactoid stacks embedded in a PEO matrix. We further elucidate the unusual confined polymer dynamics, indicating a relevant role of specific surface interactions. Y1 - 2020 U6 - http://dx.doi.org/10.1021/acs.macromol.9b01931 SN - 1520-5835 VL - 53 IS - 5 SP - 1716 EP - 1725 PB - ACS Publications CY - Washington, DC ER - TY - JOUR A1 - Monakhova, Yulia A1 - Diehl, Bernd W. K. T1 - A step towards optimization of the qNMR workflow: proficiency testing exercise at an GxP-accredited laboratory JF - Applied Magnetic Resonance N2 - Quantitative nuclear magnetic resonance (qNMR) is considered as a powerful tool for multicomponent mixture analysis as well as for the purity determination of single compounds. Special attention is currently paid to the training of operators and study directors involved in qNMR testing. To assure that only qualified personnel are used for sample preparation at our GxP-accredited laboratory, weighing test was proposed. Sixteen participants performed six-fold weighing of the binary mixture of dibutylated hydroxytoluene (BHT) and 1,2,4,5-tetrachloro-3-nitrobenzene (TCNB). To evaluate the quality of data analysis, all spectra were evaluated manually by a qNMR expert and using in-house developed automated routine. The results revealed that mean values are comparable and both evaluation approaches are free of systematic error. However, automated evaluation resulted in an approximately 20% increase in precision. The same findings were revealed for qNMR analysis of 32 compounds used in pharmaceutical industry. Weighing test by six-fold determination in binary mixtures and automated qNMR methodology can be recommended as efficient tools for evaluating staff proficiency. The automated qNMR method significantly increases throughput and precision of qNMR for routine measurements and extends application scope of qNMR. Y1 - 2021 U6 - http://dx.doi.org/10.1007/s00723-021-01324-3 SN - 1613-7507 N1 - Corresponding author: Yulia Monakhova VL - 52 SP - 581 EP - 593 PB - Springer Nature CY - Wien ER - TY - JOUR A1 - Becht, Alexander A1 - Schollmayer, Curd A1 - Monakhova, Yulia A1 - Holzgrabe, Ulrike T1 - Tracing the origin of paracetamol tablets by near-infrared, mid-infrared, and nuclear magnetic resonance spectroscopy using principal component analysis and linear discriminant analysis JF - Analytical and Bioanalytical Chemistry N2 - Most drugs are no longer produced in their own countries by the pharmaceutical companies, but by contract manufacturers or at manufacturing sites in countries that can produce more cheaply. This not only makes it difficult to trace them back but also leaves room for criminal organizations to fake them unnoticed. For these reasons, it is becoming increasingly difficult to determine the exact origin of drugs. The goal of this work was to investigate how exactly this is possible by using different spectroscopic methods like nuclear magnetic resonance and near- and mid-infrared spectroscopy in combination with multivariate data analysis. As an example, 56 out of 64 different paracetamol preparations, collected from 19 countries around the world, were chosen to investigate whether it is possible to determine the pharmaceutical company, manufacturing site, or country of origin. By means of suitable pre-processing of the spectra and the different information contained in each method, principal component analysis was able to evaluate manufacturing relationships between individual companies and to differentiate between production sites or formulations. Linear discriminant analysis showed different results depending on the spectral method and purpose. For all spectroscopic methods, it was found that the classification of the preparations to their manufacturer achieves better results than the classification to their pharmaceutical company. The best results were obtained with nuclear magnetic resonance and near-infrared data, with 94.6%/99.6% and 98.7/100% of the spectra of the preparations correctly assigned to their pharmaceutical company or manufacturer. KW - IR KW - Manufacturer KW - Linear discriminant analysis KW - Principal component analysis Y1 - 2021 U6 - http://dx.doi.org/10.1007/s00216-021-03249-z SN - 1618-2650 VL - 413 SP - 3107 EP - 3118 PB - Springer Nature ER - TY - JOUR A1 - Jablonski, Melanie A1 - Münstermann, Felix A1 - Nork, Jasmina A1 - Molinnus, Denise A1 - Muschallik, Lukas A1 - Bongaerts, Johannes A1 - Wagner, Torsten A1 - Keusgen, Michael A1 - Siegert, Petra A1 - Schöning, Michael Josef T1 - Capacitive field‐effect biosensor applied for the detection of acetoin in alcoholic beverages and fermentation broths JF - physica status solidi (a) applications and materials science N2 - An acetoin biosensor based on a capacitive electrolyte–insulator–semiconductor (EIS) structure modified with the enzyme acetoin reductase, also known as butane-2,3-diol dehydrogenase (Bacillus clausii DSM 8716ᵀ), is applied for acetoin detection in beer, red wine, and fermentation broth samples for the first time. The EIS sensor consists of an Al/p-Si/SiO₂/Ta₂O₅ layer structure with immobilized acetoin reductase on top of the Ta₂O₅ transducer layer by means of crosslinking via glutaraldehyde. The unmodified and enzyme-modified sensors are electrochemically characterized by means of leakage current, capacitance–voltage, and constant capacitance methods, respectively. KW - acetoin KW - acetoin reductase KW - alcoholic beverages KW - biosensors KW - capacitive field-effect sensors Y1 - 2021 U6 - http://dx.doi.org/10.1002/pssa.202000765 SN - 1862-6319 VL - 218 IS - 13 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Niedermeier, Jana A1 - Penner, Crystal A1 - Usherovich, Samuel A1 - Bélanger-Champagne, Camille A1 - Paulßen, Elisabeth A1 - Cornelia, Hoehr T1 - Optical Fibers as Dosimeter Detectors for Mixed Proton/Neutron Fields - A Biological Dosimeter JF - electronics N2 - In recent years, proton therapy has gained importance as a cancer treatment modality due to its conformality with the tumor and the sparing of healthy tissue. However, in the interaction of the protons with the beam line elements and patient tissues, potentially harmful secondary neutrons are always generated. To ensure that this neutron dose is as low as possible, treatment plans could be created to also account for and minimize the neutron dose. To monitor such a treatment plan, a compact, easy to use, and inexpensive dosimeter must be developed that not only measures the physical dose, but which can also distinguish between proton and neutron contributions. To that end, plastic optical fibers with scintillation materials (Gd₂O₂S:Tb, Gd₂O₂S:Eu, and YVO₄:Eu) were irradiated with protons and neutrons. It was confirmed that sensors with different scintillation materials have different sensitivities to protons and neutrons. A combination of these three scintillators can be used to build a detector array to create a biological dosimeter. KW - biological dosimeter KW - Bragg peak KW - relative dosimetry KW - optical fibers KW - proton therapy KW - protons KW - neutrons Y1 - 2023 U6 - http://dx.doi.org/10.3390/electronics12020324 SN - 2079-9292 N1 - This article belongs to the Special Issue "Applications of Optical Fiber Sensors" VL - 12 IS - 2 PB - MDPI CY - Basel ER - TY - JOUR A1 - Penner, Crystal A1 - Usherovich, Samuel A1 - Niedermeier, Jana A1 - Bélanger-Champagne, Camille A1 - Trinczek, Michael A1 - Paulßen, Elisabeth A1 - Hoehr, Cornelia T1 - Organic Scintillator-Fibre Sensors for Proton Therapy Dosimetry: SCSF-3HF and EJ-260 JF - electronics N2 - In proton therapy, the dose from secondary neutrons to the patient can contribute to side effects and the creation of secondary cancer. A simple and fast detection system to distinguish between dose from protons and neutrons both in pretreatment verification as well as potentially in vivo monitoring is needed to minimize dose from secondary neutrons. Two 3 mm long, 1 mm diameter organic scintillators were tested for candidacy to be used in a proton–neutron discrimination detector. The SCSF-3HF (1500) scintillating fibre (Kuraray Co. Chiyoda-ku, Tokyo, Japan) and EJ-260 plastic scintillator (Eljen Technology, Sweetwater, TX, USA) were irradiated at the TRIUMF Neutron Facility and the Proton Therapy Research Centre. In the proton beam, we compared the raw Bragg peak and spread-out Bragg peak response to the industry standard Markus chamber detector. Both scintillator sensors exhibited quenching at high LET in the Bragg peak, presenting a peak-to-entrance ratio of 2.59 for the EJ-260 and 2.63 for the SCSF-3HF fibre, compared to 3.70 for the Markus chamber. The SCSF-3HF sensor demonstrated 1.3 times the sensitivity to protons and 3 times the sensitivity to neutrons as compared to the EJ-260 sensor. Combined with our equations relating neutron and proton contributions to dose during proton irradiations, and the application of Birks’ quenching correction, these fibres provide valid candidates for inexpensive and replicable proton-neutron discrimination detectors Y1 - 2022 U6 - http://dx.doi.org/10.3390/electronics12010011 SN - 2079-9292 N1 - This article belongs to the Special Issue "Applications of Optical Fiber Sensors" VL - 12 IS - 1 PB - MDPI CY - Basel ER - TY - JOUR A1 - Haeger, Gerrit A1 - Grankin, Alina A1 - Wagner, Michaela T1 - Construction of an Aspergillus oryzae triple amylase deletion mutant as a chassis to evaluate industrially relevant amylases using multiplex CRISPR/Cas9 editing technology JF - Applied Research N2 - Aspergillus oryzae is an industrially relevant organism for the secretory production of heterologous enzymes, especially amylases. The activities of potential heterologous amylases, however, cannot be quantified directly from the supernatant due to the high background activity of native α-amylase. This activity is caused by the gene products of amyA, amyB, and amyC. In this study, an in vitro CRISPR/Cas9 system was established in A. oryzae to delete these genes simultaneously. First, pyrG of A. oryzae NSAR1 was mutated by exploiting NHEJ to generate a counter-selection marker. Next, all amylase genes were deleted simultaneously by co-transforming a repair template carrying pyrG of Aspergillus nidulans and flanking sequences of amylase gene loci. The rate of obtained triple knock-outs was 47%. We showed that triple knockouts do not retain any amylase activity in the supernatant. The established in vitro CRISPR/Cas9 system was used to achieve sequence-specific knock-in of target genes. The system was intended to incorporate a single copy of the gene of interest into the desired host for the development of screening methods. Therefore, an integration cassette for the heterologous Fpi amylase was designed to specifically target the amyB locus. The site-specific integration rate of the plasmid was 78%, with exceptional additional integrations. Integration frequency was assessed via qPCR and directly correlated with heterologous amylase activity. Hence, we could compare the efficiency between two different signal peptides. In summary, we present a strategy to exploit CRISPR/Cas9 for gene mutation, multiplex knock-out, and the targeted knock-in of an expression cassette in A. oryzae. Our system provides straightforward strain engineering and paves the way for development of fungal screening systems. KW - aspergillus KW - CRISPR/Cas9 KW - filamentous fungi KW - genome engineering Y1 - 2023 U6 - http://dx.doi.org/10.1002/appl.202200106 SN - 2702-4288 IS - Early View SP - 1 EP - 15 PB - Wiley-VCH ER - TY - JOUR A1 - Hoffstadt, Kevin A1 - Nikolausz, Marcell A1 - Krafft, Simone A1 - Bonatelli, Maria A1 - Kumar, Vivekanantha A1 - Harms, Hauke A1 - Kuperjans, Isabel T1 - Optimization of the ex situ biomethanation of hydrogen and carbon dioxide in a novel meandering plug flow reactor: start-up phase and flexible operation JF - Bioengineering KW - methanation KW - plug flow reactor KW - bubble column KW - biomethane KW - P2G Y1 - 2024 U6 - http://dx.doi.org/10.3390/bioengineering11020165 SN - 2306-5354 VL - 11 IS - 2 PB - MDPI CY - Basel ER - TY - JOUR A1 - Block, Franziska A1 - May, Alexander A1 - Wetzel, Katharina A1 - Adels, Klaudia A1 - Elbers, Gereon A1 - Schulze, Margit A1 - Monakhova, Yulia T1 - What is the best spectroscopic method for simultaneous analysis of organic acids and (poly)saccharides in biological matrices: Example of Aloe vera extracts? JF - Talanta Open N2 - Several species of (poly)saccharides and organic acids can be found often simultaneously in various biological matrices, e.g., fruits, plant materials, and biological fluids. The analysis of such matrices sometimes represents a challenging task. Using Aloe vera (A. vera) plant materials as an example, the performance of several spectro-scopic methods (80 MHz benchtop NMR, NIR, ATR-FTIR and UV–vis) for the simultaneous analysis of quality parameters of this plant material was compared. The determined parameters include (poly)saccharides such as aloverose, fructose and glucose as well as organic acids (malic, lactic, citric, isocitric, acetic, fumaric, benzoic and sorbic acids). 500 MHz NMR and high-performance liquid chromatography (HPLC) were used as the reference methods. UV–vis data can be used only for identification of added preservatives (benzoic and sorbic acids) and drying agent (maltodextrin) and semiquantitative analysis of malic acid. NIR and MIR spectroscopies combined with multivariate regression can deliver more informative overview of A. vera extracts being able to additionally quantify glucose, aloverose, citric, isocitric, malic, lactic acids and fructose. Low-field NMR measurements can be used for the quantification of aloverose, glucose, malic, lactic, acetic, and benzoic acids. The benchtop NMR method was successfully validated in terms of robustness, stability, precision, reproducibility and limit of detection (LOD) and quantification (LOQ), respectively. All spectroscopic techniques are useful for the screening of (poly)saccharides and organic acids in plant extracts and should be applied according to its availability as well as information and confidence required for the specific analytical goal. Benchtop NMR spectroscopy seems to be the most feasible solution for quality control of A. vera products. KW - Spectroscopy KW - (Poly)saccharides KW - Organic acids KW - Aloe vera KW - qNMR Y1 - 2023 U6 - http://dx.doi.org/10.1016/j.talo.2023.100220 SN - 2666-8319 VL - 7 IS - Art. No. 100220 SP - 1 EP - 9 PB - Elsevier CY - Amsterdam ER - TY - RPRT A1 - Haeger, Gerrit A1 - Bongaerts, Johannes A1 - Siegert, Petra T1 - Abschlussbericht Teil II: Eingehende Darstellung Neue biobasierte Lipopeptide aus nachhaltiger Produktion (LipoPep) Y1 - 2023 N1 - Förderkennzeichen: 13FH256PA6 Titel: FHprofUnt 2016: Neue biobasierte Lipopeptide aus nachhaltiger Produktion Laufzeit: 01.02.2019 – 31.10.2022 ER - TY - RPRT A1 - Siegert, Petra A1 - Bongaerts, Johannes A1 - Wagner, Torsten A1 - Schöning, Michael Josef A1 - Selmer, Thorsten T1 - Abschlussbericht zum Projekt zur Überwachung biotechnologischer Prozesse mittels Diacetyl-/Acetoin-Biosensor und Evaluierung von Acetoin-Reduktasen zur Verwendung in Biotransformationen Y1 - 2022 N1 - Laufzeit: 01.01.2016 – 31.12.2019 (verlängert bis 31.12.2020) Förderkennzeichen: 322-8.03.04.02-FH-Struktur 2016/02 Gefördert durch: Ministerium für Innovation, Wissenschaft und Forschung des Landes Nordrhein-Westfalen CY - Aachen ER - TY - JOUR A1 - Trapp, Svenja A1 - Lammers, Tom A1 - Engudar, Gokce A1 - Hoehr, Cornelia A1 - Denkova, Antonia G. A1 - Paulßen, Elisabeth A1 - de Kruijff, Robin M. T1 - Membrane-based microfluidic solvent extraction of Ga-68 from aqueous Zn solutions: towards an automated cyclotron production loop JF - EJNMMI Radiopharmacy and Chemistry KW - Microfluidic solvent extraction KW - Ga-68 KW - Cyclotron production KW - Medical radionuclide production KW - Metal contaminants Y1 - 2023 U6 - http://dx.doi.org/10.1186/s41181-023-00195-2 SN - 2365-421X VL - 2023 IS - 8, Article number: 9 SP - 1 EP - 14 PB - Springer Nature ER - TY - JOUR A1 - Falkenberg, Fabian A1 - Voß, Leonie A1 - Bott, Michael A1 - Bongaerts, Johannes A1 - Siegert, Petra T1 - New robust subtilisins from halotolerant and halophilic Bacillaceae JF - Applied Microbiology and Biotechnology N2 - The aim of the present study was the characterisation of three true subtilisins and one phylogenetically intermediate subtilisin from halotolerant and halophilic microorganisms. Considering the currently growing enzyme market for efficient and novel biocatalysts, data mining is a promising source for novel, as yet uncharacterised enzymes, especially from halophilic or halotolerant Bacillaceae, which offer great potential to meet industrial needs. Both halophilic bacteria Pontibacillus marinus DSM 16465ᵀ and Alkalibacillus haloalkaliphilus DSM 5271ᵀ and both halotolerant bacteria Metabacillus indicus DSM 16189 and Litchfieldia alkalitelluris DSM 16976ᵀ served as a source for the four new subtilisins SPPM, SPAH, SPMI and SPLA. The protease genes were cloned and expressed in Bacillus subtilis DB104. Purification to apparent homogeneity was achieved by ethanol precipitation, desalting and ion-exchange chromatography. Enzyme activity could be observed between pH 5.0–12.0 with an optimum for SPPM, SPMI and SPLA around pH 9.0 and for SPAH at pH 10.0. The optimal temperature for SPMI and SPLA was 70 °C and for SPPM and SPAH 55 °C and 50 °C, respectively. All proteases showed high stability towards 5% (w/v) SDS and were active even at NaCl concentrations of 5 M. The four proteases demonstrate potential for future biotechnological applications. KW - Biotechnological application KW - Bacillaceae KW - Subtilisin KW - Subtilases KW - Halotolerant protease Y1 - 2023 U6 - http://dx.doi.org/10.1007/s00253-023-12553-w SN - 1432-0614 N1 - Corresponding author: Petra Siegert VL - 107 SP - 3939 EP - 3954 PB - Springer Nature CY - Berlin ER - TY - INPR A1 - Greiner, Lasse A1 - Jeromin, Günter Erich A1 - Sithole, Patience A1 - Petersen, Soenke T1 - Preprint: Studies on the enzymatic reduction of levulinic acid using Chiralidon-R and Chiralidon-S T2 - ChemRxiv N2 - The enzymatic reduction of levulinic acid by the chiral catalysts Chiralidon-R and Chiralidon-S which are commercially available superabsorbed alcohol dehydrogenases is described. The Chiralidon®-R/S reduces the levulinic acid to the (R,S)-4-hydroxy valeric acid and the (R)- or (S)- gamma-valerolactone. KW - Levulinic acid KW - Chiralidon-R KW - Chiralidon-S KW - 4-hydroxy valeric acid KW - (R)- or (S)- gamma-valerolactone Y1 - 2023 U6 - http://dx.doi.org/10.26434/chemrxiv-2023-jlvcv ER - TY - JOUR A1 - Adels, Klaudia A1 - Elbers, Gereon A1 - Diehl, Bernd A1 - Monakhova, Yulia T1 - Multicomponent analysis of dietary supplements containing glucosamine and chondroitin: comparative low- and high-field NMR spectroscopic study JF - Analytical Sciences N2 - With the prevalence of glucosamine- and chondroitin-containing dietary supplements for people with osteoarthritis in the marketplace, it is important to have an accurate and reproducible analytical method for the quantitation of these compounds in finished products. NMR spectroscopic method based both on low- (80 MHz) and high- (500–600 MHz) field NMR instrumentation was established, compared and validated for the determination of chondroitin sulfate and glucosamine in dietary supplements. The proposed method was applied for analysis of 20 different dietary supplements. In the majority of cases, quantification results obtained on the low-field NMR spectrometer are similar to those obtained with high-field 500–600 MHz NMR devices. Validation results in terms of accuracy, precision, reproducibility, limit of detection and recovery demonstrated that the developed method is fit for purpose for the marketed products. The NMR method was extended to the analysis of methylsulfonylmethane, adulterant maltodextrin, acetate and inorganic ions. Low-field NMR can be a quicker and cheaper alternative to more expensive high-field NMR measurements for quality control of the investigated dietary supplements. High-field NMR instrumentation can be more favorable for samples with complex composition due to better resolution, simultaneously giving the possibility of analysis of inorganic species such as potassium and chloride. KW - Glucosamine KW - Chondroitin sulfate KW - Polysaccharides KW - Dietary supplements KW - High-field NMR Y1 - 2023 U6 - http://dx.doi.org/10.1007/s44211-023-00433-2 SN - 1348-2246 (Online) SN - 0910-6340 (Print) N1 - Corresponding author: Yulia Monakhova VL - 2023 PB - Springer Verlag CY - Cham ER - TY - JOUR A1 - Falkenberg, Fabian A1 - Kohn, Sophie A1 - Bott, Michael A1 - Bongaerts, Johannes A1 - Siegert, Petra T1 - Biochemical characterisation of a novel broad pH spectrum subtilisin from Fictibacillus arsenicus DSM 15822ᵀ JF - FEBS Open Bio N2 - Subtilisins from microbial sources, especially from the Bacillaceae family, are of particular interest for biotechnological applications and serve the currently growing enzyme market as efficient and novel biocatalysts. Biotechnological applications include use in detergents, cosmetics, leather processing, wastewater treatment and pharmaceuticals. To identify a possible candidate for the enzyme market, here we cloned the gene of the subtilisin SPFA from Fictibacillus arsenicus DSM 15822ᵀ (obtained through a data mining-based search) and expressed it in Bacillus subtilis DB104. After production and purification, the protease showed a molecular mass of 27.57 kDa and a pI of 5.8. SPFA displayed hydrolytic activity at a temperature optimum of 80 °C and a very broad pH optimum between 8.5 and 11.5, with high activity up to pH 12.5. SPFA displayed no NaCl dependence but a high NaCl tolerance, with decreasing activity up to concentrations of 5 m NaCl. The stability enhanced with increasing NaCl concentration. Based on its substrate preference for 10 synthetic peptide 4-nitroanilide substrates with three or four amino acids and its phylogenetic classification, SPFA can be assigned to the subgroup of true subtilisins. Moreover, SPFA exhibited high tolerance to 5% (w/v) SDS and 5% H₂O₂ (v/v). The biochemical properties of SPFA, especially its tolerance of remarkably high pH, SDS and H₂O₂, suggest it has potential for biotechnological applications. KW - Bacillaceae KW - Biotechnological application KW - Broad pH spectrum KW - Subtilases KW - Subtilisin Y1 - 2023 U6 - http://dx.doi.org/10.1002/2211-5463.13701 SN - 2211-5463 N1 - Corresponding author: Petra Siegert VL - 13 IS - 11 SP - 2035 EP - 2046 PB - Wiley CY - Hoboken, NJ ER - TY - JOUR A1 - Dellmann, Sophia Florence A1 - Glorius, J. A1 - Litvinov, Yu A. A1 - Reifarth, R. A1 - Al-Khasawneh, Kafa A1 - Aliotta, M. A1 - Bott, L. A1 - Brückner, Benjamin A1 - Bruno, C. G. A1 - Chen, Ruijiu A1 - Davinson, T. A1 - Dickel, T. A1 - Dillmann, Iris A1 - Dmytriev, D. A1 - Erbacher, P. A1 - Freire-Fernández, D. A1 - Forstner, Oliver A1 - Geissel, H. A1 - Göbel, K. A1 - Griffin, Christopher J. A1 - Grisenti, R. A1 - Gumberidze, Alexandre A1 - Haettner, Emma A1 - Hagmann, Siegbert A1 - Heil, M. A1 - Heß, R. A1 - Hillenbrand, P.-M. A1 - Joseph, R. A1 - Jurado, B. A1 - Kozhuharov, Christophor A1 - Kulikov, I. A1 - Löher, Bastian A1 - Langer, Christoph A1 - Leckenby, Guy A1 - Lederer-Woods, C. A1 - Lestinsky, M. A1 - Litvinov, S. A. A1 - Lorenz, B. A. A1 - Lorenz, E. A1 - Marsh, J. A1 - Menz, Esther Babette A1 - Morgenroth, T. A1 - Petridis, N. A1 - Pibernat, Jerome A1 - Popp, U. A1 - Psaltis, Athanasios A1 - Sanjari, Shahab A1 - Scheidenberger, C. A1 - Sguazzin, M. A1 - Sidhu, Ragandeep Singh A1 - Spillmann, Uwe A1 - Steck, M. A1 - Stöhlker, T. A1 - Surzhykov, A. A1 - Swartz, J. A. A1 - Törnqvist, H. A1 - Varga, L. A1 - Vescovi, Diego A1 - Weick, H. A1 - Weigand, M. A1 - Woods, P. A1 - Xing, Y. A1 - Yamaguchi, Taiyo T1 - Proton capture on stored radioactive ¹¹⁸Te ions JF - EPJ Web of Conferences N2 - Experimental determination of the cross sections of proton capture on radioactive nuclei is extremely difficult. Therefore, it is of substantial interest for the understanding of the production of the p-nuclei. For the first time, a direct measurement of proton-capture cross sections on stored, radioactive ions became possible in an energy range of interest for nuclear astrophysics. The experiment was performed at the Experimental Storage Ring (ESR) at GSI by making use of a sensitive method to measure (p,γ) and (p,n) reactions in inverse kinematics. These reaction channels are of high relevance for the nucleosyn-thesis processes in supernovae, which are among the most violent explosions in the universe and are not yet well understood. The cross section of the ¹¹⁸Te(p,γ) reaction has been measured at energies of 6 MeV/u and 7 MeV/u. The heavy ions interacted with a hydrogen gas jet target. The radiative recombination process of the fully stripped ¹¹⁸Te ions and electrons from the hydrogen target was used as a luminosity monitor. An overview of the experimental method and preliminary results from the ongoing analysis will be presented. Y1 - 2023 U6 - http://dx.doi.org/10.1051/epjconf/202327911018 SN - 2100-014X N1 - Volume 279, 2023. Nuclear Physics in Astrophysics – X (NPA-X 2022). VL - 279 IS - Article Number: 11018 SP - 1 EP - 5 PB - EDP Sciences ER - TY - JOUR A1 - Hoffstadt, Kevin A1 - Cheenakula, Dheeraja A1 - Nikolausz, Marcell A1 - Krafft, Simone A1 - Harms, Hauke A1 - Kuperjans, Isabel T1 - Design and construction of a new reactor for flexible biomethanation of hydrogen JF - Fermentation N2 - The increasing share of renewable electricity in the grid drives the need for sufficient storage capacity. Especially for seasonal storage, power-to-gas can be a promising approach. Biologically produced methane from hydrogen produced from surplus electricity can be used to substitute natural gas in the existing infrastructure. Current reactor types are not or are poorly optimized for flexible methanation. Therefore, this work proposes a new reactor type with a plug flow reactor (PFR) design. Simulations in COMSOL Multiphysics ® showed promising properties for operation in laminar flow. An experiment was conducted to support the simulation results and to determine the gas fraction of the novel reactor, which was measured to be 29%. Based on these simulations and experimental results, the reactor was constructed as a 14 m long, 50 mm diameter tube with a meandering orientation. Data processing was established, and a step experiment was performed. In addition, a kLa of 1 h−1 was determined. The results revealed that the experimental outcomes of the type of flow and gas fractions are in line with the theoretical simulation. The new design shows promising properties for flexible methanation and will be tested. KW - methanation KW - plug flow reactor KW - bubble column KW - bio-methane KW - power-to-gas Y1 - 2023 U6 - http://dx.doi.org/10.3390/fermentation9080774 SN - 2311-5637 N1 - The article belongs to the Special Issue Fermentation Processes: Modeling, Optimization and Control VL - 9 IS - 8 SP - 1 EP - 16 PB - MDPI CY - Basel ER - TY - JOUR A1 - El Bergui, Omnia A1 - Abouabdillah, Aziz A1 - Bourioug, Mohamed A1 - Schmitz, Dominik A1 - Biel, Markus A1 - Aboudrare, Abdellah A1 - Krauss, Manuel A1 - Jomaa, Ahlem A1 - Romuli, Sebastian A1 - Müller, Joachim A1 - Fagroud, Mustapha A1 - Bouabid, Rachid T1 - Innovative solutions for drought: Evaluating hydrogel application on onion cultivation (Allium cepa) in Morocco JF - Water N2 - Throughout the last decade, and particularly in 2022, water scarcity has become a critical concern in Morocco and other Mediterranean countries. The lack of rainfall during spring was worsened by a succession of heat waves during the summer. To address this drought, innovative solutions, including the use of new technologies such as hydrogels, will be essential to transform agriculture. This paper presents the findings of a study that evaluated the impact of hydrogel application on onion (Allium cepa) cultivation in Meknes, Morocco. The treatments investigated in this study comprised two different types of hydrogel-based soil additives (Arbovit® polyacrylate and Huminsorb® polyacrylate), applied at two rates (30 and 20 kg/ha), and irrigated at two levels of water supply (100% and 50% of daily crop evapotranspiration; ETc). Two control treatments were included, without hydrogel application and with both water amounts. The experiment was conducted in an open field using a completely randomized design. The results indicated a significant impact of both hydrogel-type dose and water dose on onion plant growth, as evidenced by various vegetation parameters. Among the hydrogels tested, Huminsorb® Polyacrylate produced the most favorable outcomes, with treatment T9 (100%, HP, 30 kg/ha) yielding 70.55 t/ha; this represented an increase of 11 t/ha as compared to the 100% ETc treatment without hydrogel application. Moreover, the combination of hydrogel application with 50% ETc water stress showed promising results, with treatment T4 (HP, 30 kg, 50%) producing almost the same yield as the 100% ETc treatment without hydrogel while saving 208 mm of water. KW - water economy KW - yield KW - deficit irrigation KW - hydrogel KW - onion Y1 - 2023 U6 - http://dx.doi.org/10.3390/w15111972 VL - 15 IS - 11 PB - MDPI CY - Basel ER - TY - JOUR A1 - Haeger, Gerrit A1 - Probst, Johanna A1 - Jaeger, Karl-Erich A1 - Bongaerts, Johannes A1 - Siegert, Petra T1 - Novel aminoacylases from Streptomyces griseus DSM 40236 and their recombinant production in Streptomyces lividans JF - FEBS Open Bio N2 - Amino acid-based surfactants are valuable compounds for cosmetic formulations. The chemical synthesis of acyl-amino acids is conventionally performed by the Schotten-Baumann reaction using fatty acyl chlorides, but aminoacylases have also been investigated for use in biocatalytic synthesis with free fatty acids. Aminoacylases and their properties are diverse; they belong to different peptidase families and show differences in substrate specificity and biocatalytic potential. Bacterial aminoacylases capable of synthesis have been isolated from Burkholderia, Mycolicibacterium, and Streptomyces. Although several proteases and peptidases from S. griseus have been described, no aminoacylases from this species have been identified yet. In this study, we investigated two novel enzymes produced by S. griseus DSM 40236ᵀ . We identified and cloned the respective genes and recombinantly expressed an α-aminoacylase (EC 3.5.1.14), designated SgAA, and an ε-lysine acylase (EC 3.5.1.17), designated SgELA, in S. lividans TK23. The purified aminoacylase SgAA was biochemically characterized, focusing on its hydrolytic activity to determine temperature- and pH optima and stabilities. The aminoacylase could hydrolyze various acetyl-amino acids at the Nα -position with a broad specificity regarding the sidechain. Substrates with longer acyl chains, like lauroyl-amino acids, were hydrolyzed to a lesser extent. Purified aminoacylase SgELA specific for the hydrolysis of Nε -acetyl-L-lysine was unstable and lost its enzymatic activity upon storage for a longer period but could initially be characterized. The pH optimum of SgELA was pH 8.0. While synthesis of acyl-amino acids was not observed with SgELA, SgAA catalyzed the synthesis of lauroyl-methionine. KW - Streptomyces lividans KW - recombinant expression KW - Streptomyces griseus KW - ε-lysine acylase KW - α-aminoacylase Y1 - 2023 U6 - http://dx.doi.org/10.1002/2211-5463.13723 SN - 2211-5463 N1 - Corresponding author: Petra Siegert VL - 13 IS - 12 SP - 2224 EP - 2238 PB - Wiley CY - Hoboken, NJ ER - TY - BOOK A1 - Lauth, Jakob T1 - Physical chemistry in a nutshell: Basics for engineers and scientists N2 - This book is based on a multimedia course for biological and chemical engineers, which is designed to trigger students' curiosity and initiative. A solid basic knowledge of thermodynamics and kinetics is necessary for understanding many technical, chemical, and biological processes. The one-semester basic lecture course was divided into 12 workshops (chapters). Each chapter covers a practically relevant area of physical chemistry and contains the following didactic elements that make this book particularly exciting and understandable: - Links to Videos at the start of each chapter as preparation for the workshop - Key terms (in bold) for further research of your own - Comprehension questions and calculation exercises with solutions as learning checks - Key illustrations as simple, easy-to-replicate blackboard pictures Humorous cartoons for each workshop (by Faelis) additionally lighten up the text and facilitate the learning process as a mnemonic. To round out the book, the appendix includes a summary of the most popular experiments in basic physical chemistry courses, as well as suggestions for designing workshops with exhibits, experiments, and "questions of the day." Suitable for students minoring in chemistry; chemistry majors are sure to find this slimmed-down, didactically valuable book helpful as well. The book is excellent for self-study. KW - Physical chemistry KW - Thermodynamics as minor KW - Physical chemistry starters KW - Minor chemistry KW - Physical chemistry basics Y1 - 2023 SN - 978-3-662-67636-3 (Softcover) SN - 978-3-662-67637-0 (eBook) U6 - http://dx.doi.org/10.1007/978-3-662-67637-0 PB - Springer CY - Berlin ER - TY - JOUR A1 - Barbazán, Paula A1 - Hagenbach, Adelheid A1 - Paulßen, Elisabeth A1 - Abram, Ulrich A1 - Carballo, Rosa A1 - Rodriguez-Hermida, Sabina A1 - Vázquez-López, Ezequiel M. T1 - Tricarbonyl Rhenium(I) and Technetium(I) Complexes with Hydrazones Derived from 4,5-Diazafluoren-9-one and 1,10-Phenanthroline-5,6-dione JF - European Journal of Inorganic Chemistry N2 - Tricarbonylrhenium(I) and -technetium(I) halide (halide = Cl and Br) complexes of ligands derived from 4,5-diazafluoren-9-one (df) and 1,10-phenanthroline-5,6-dione (phen) derivatives of benzoic and 2-hydroxybenzoic acid hydrazides have been prepared. The complexes have been characterized by elemental analysis, MS, IR, 1H NMR and absorption and emission UV/Vis spectroscopic methods. The metal centres (ReI and TcI) are coordinated through the nitrogen imine atoms and establish five-membered chelate rings, whereas the hydrazone groups stand uncoordinated. The 1H NMR spectra suggest the same behaviour in solution on the basis of only marginal variations in the chemical shifts of the hydrazine protons. Y1 - 2010 U6 - http://dx.doi.org/10.1002/ejic.201000522 SN - 1099-0682 IS - 29 SP - 4622 EP - 4630 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Paulßen, Elisabeth A1 - Schweighöfer, Philip V. A1 - Abram, Ulrich T1 - Reactions of [ReOX3(PPh3)2] Complexes (X = Cl, Br) with Phenylacetylene and the Structures of the Products JF - Zeitschrift für anorganische und allgemeine Chemie : ZAAC = Journal of inorganic and general chemistry N2 - Oxorhenium(V) complexes [ReOX3(PPh3)2] (X = Cl, Br) react with phenylacetylene under formation of complexes with ylide-type ligands. Compounds of the compositions [ReOCl3(PPh3){C(Ph)C(H)(PPh3)}] (1), [ReOBr3(OPPh3){C(Ph)C(H)(PPh3)}] (2), and [ReOBr3(OPPh3){C(H)C(Ph)(PPh3)}] (3) were isolated and characterized by X-ray diffraction. They contain a ligand, which was formed by a nucleophilic attack of released PPh3 at coordinated phenylacetylene. The structures of the products show that there is no preferable position for this attack. Cleavage of the Re–C bond in 3 and dimerization of the organic ligand resulted in the formation of the [{(PPh3)(H)CC(Ph)}2]2+ cation, which crystallized as its [(ReOBr4)(OReO3)]2– salt. Y1 - 2010 U6 - http://dx.doi.org/10.1002/zaac.200900478 SN - 1521-3749 VL - 636 IS - 5 SP - 779 EP - 783 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Paulßen, Elisabeth A1 - Alberto, Roger A1 - Abram, Ulrich T1 - Synthesis, Characterization, and Structures of R3EOTcO3 Complexes (E = C, Si, Ge, Sn, Pb) and Related Compounds JF - Inorganic Chemistry N2 - AgTcO4 reacts with R3ECl compounds (E = C, Si, Ge, Sn, Pb; R = Me, iPr, tBu, Ph), tBu2SnCl2, or PhMgCl under formation of novel trioxotechnetium(VII) derivatives. The carbon and silicon derivatives readily undergo decomposition, which was proven by 99Tc NMR spectroscopy and the isolation of decomposition products such as [TcOCl3(THF)(OH2)]. Compounds [Ph3GeOTcO3], [(THF)Ph3SnOTcO3], [(O3TcO)SntBu2(OH)]2, and [(THF)4Mg(OTcO3)2] are more stable and were isolated in crystalline form and characterized by X-ray diffraction. Y1 - 2010 U6 - http://dx.doi.org/10.1021/ic1001094 SN - 1520-510X VL - 49 IS - 7 SP - 3525 EP - 3530 PB - American Chemical Society CY - Washington ER - TY - JOUR A1 - Tippkötter, Nils A1 - Roikaew, Wipa A1 - Ulber, Roland A1 - Hoffmann, Alexander A1 - Denzler, Hans-Jörg A1 - Buchholz, Heinrich T1 - Paracoccus denitrificans for the effluent recycling during continuous denitrification of liquid food JF - Biotechnology Progress N2 - Nitrate is an undesirable component of several foods. A typical case of contamination with high nitrate contents is whey concentrate, containing nitrate in concentrations up to 25 l. The microbiological removal of nitrate by Paracoccus denitrificans under formation of harmless nitrogen in combination with a cell retention reactor is described here. Focus lies on the resource-conserving design of a microbal denitrification process. Two methods are compared. The application of polyvinyl alcohol-immobilized cells, which can be applied several times in whey feed, is compared with the implementation of a two step denitrification system. First, the whey concentrate's nitrate is removed by ion exchange and subsequently the eluent regenerated by microorganisms under their retention by crossflow filtration. Nitrite and nitrate concentrations were determined by reflectometric color measurement with a commercially available Reflectoquant® device. Correction factors for these media had to be determined. During the pilot development, bioreactors from 4 to 250 mg·L-1 and crossflow units with membrane areas from 0.02 to 0.80 m2 were examined. Based on the results of the pilot plants, a scaling for the exemplary process of denitrifying 1,000 tons per day is discussed. Y1 - 2010 U6 - http://dx.doi.org/10.1002/btpr.384 SN - 8756-7938 VL - 26 IS - 3 SP - 756 EP - 762 PB - Wiley CY - Hoboken, NJ ER - TY - JOUR A1 - Ulber, Roland A1 - Poth, Sebastian A1 - Monzon, Magaly A1 - Tippkötter, Nils T1 - Prozessintegration von Hydrolyse und Fermentation von Cellulose- Faserstoff JF - Chemie Ingenieur Technik N2 - Ein viel versprechender erneuerbarer Rohstoff für die Produktion von Chemikalien und Treibstoffen ist Lignocellulose aus pflanzlicher Biomasse. Die darin enthaltenen Zucker können mittels enzymatischer Hydrolyse freigesetzt und fermentativ zu Ethanol umgesetzt werden. Ein interessanter Ansatz ist dabei die simultane Verzuckerung und Fermentation. Hefen und Enzyme haben mit 30 °C bzw. 50 °C zwar unterschiedliche Temperaturoptima, es konnte aber gezeigt werden, dass auch bei den niedrigeren Temperaturen eine Umsetzung der Cellulose zu Glucose erfolgt, wenn auch langsamer als bei optimalen Bedingungen. Außerdem konnte in Vorversuchen gezeigt werden, dass Ethanol in den zu erwartenden Konzentrationen keinen Einfluss auf die enzymatische Umsetzung hat. Y1 - 2010 U6 - http://dx.doi.org/10.1002/cite.200900103 SN - 1522-2640 N1 - Special Issue "Biokatalyse" VL - 82 IS - 1-2 SP - 135 EP - 139 ER - TY - JOUR A1 - Sieker, Tim A1 - Neuner, Andreas A1 - Dimitrova, Darina A1 - Tippkötter, Nils A1 - Bart, Hans-Jörg A1 - Heinzle, Elmar A1 - Ulber, Roland T1 - Grassilage als Rohstoff für die chemische Industrie JF - Chemie Ingenieur Technik N2 - Grassilage stellt einen nachwachsenden Rohstoff mit großem Potenzial dar. Neben Cellulose und Hemicellulose enthält sie auch organische Säuren, insbesondere Milchsäure. In einem Bioraffinerie-Projekt wird die Milchsäure aus der Silage isoliert und mit gentechnisch optimierten Stämmen zu L-Lysin weiterverarbeitet. Die Lignocellulose wird hydrolysiert und zu Ethanol fermentiert. Ein besonderes Augenmerk liegt auf der Integration der unterschiedlichen Prozesse sowie der einzelnen Prozessschritte zu einem Gesamtprozess, der sämtliche Inhaltsstoffe der Silage verwertet. Y1 - 2010 U6 - http://dx.doi.org/10.1002/cite.201000088 SN - 1522-2640 VL - 82 IS - 8, Special Issue: Industrielle Nutzung nachwachsender Rohstoffe SP - 1153 EP - 1159 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Degering, Christian A1 - Eggert, Thorsten A1 - Puls, Michael A1 - Bongaerts, Johannes A1 - Evers, Stefan A1 - Maurer, Karl-Heinz A1 - Jaeger, Karl-Erich T1 - Optimization of protease secretion in Bacillus subtilis and Bacillus licheniformis by screening of homologous and herologous signal peptides JF - Applied and environmental microbiology N2 - Bacillus subtilis and Bacillus licheniformis are widely used for the large-scale industrial production of proteins. These strains can efficiently secrete proteins into the culture medium using the general secretion (Sec) pathway. A characteristic feature of all secreted proteins is their N-terminal signal peptides, which are recognized by the secretion machinery. Here, we have studied the production of an industrially important secreted protease, namely, subtilisin BPN′ from Bacillus amyloliquefaciens. One hundred seventy-three signal peptides originating from B. subtilis and 220 signal peptides from the B. licheniformis type strain were fused to this secretion target and expressed in B. subtilis, and the resulting library was analyzed by high-throughput screening for extracellular proteolytic activity. We have identified a number of signal peptides originating from both organisms which produced significantly increased yield of the secreted protease. Interestingly, we observed that levels of extracellular protease were improved not only in B. subtilis, which was used as the screening host, but also in two different B. licheniformis strains. To date, it is impossible to predict which signal peptide will result in better secretion and thus an improved yield of a given extracellular target protein. Our data show that screening a library consisting of homologous and heterologous signal peptides fused to a target protein can identify more-effective signal peptides, resulting in improved protein export not only in the original screening host but also in different production strains. Y1 - 2010 U6 - http://dx.doi.org/10.1128/AEM.01146-10 SN - 1098-5336 (E-Journal); 0003-6919 (Print); 0099-2240 (Print) VL - 76 IS - 19 SP - 6370 EP - 6378 PB - American Society for Microbiology CY - Washington, DC ER - TY - JOUR A1 - Schroeter, Rebecca A1 - Hoffmann, Tamara A1 - Voigt, Birgit A1 - Meyer, Hanna A1 - Bleisteiner, Monika A1 - Muntel, Jan A1 - Jürgen, Britta A1 - Albrecht, Dirk A1 - Becher, Dörte A1 - Lalk, Michael A1 - Evers, Stefan A1 - Bongaerts, Johannes A1 - Maurer, Karl-Heinz A1 - Putzer, Harald A1 - Hecker, Michael A1 - Schweder, Thomas A1 - Bremer, Erhard T1 - Stress responses of the industrial workhorse Bacillus licheniformis to osmotic challenges JF - PLoS ONE N2 - The Gram-positive endospore-forming bacterium Bacillus licheniformis can be found widely in nature and it is exploited in industrial processes for the manufacturing of antibiotics, specialty chemicals, and enzymes. Both in its varied natural habitats and in industrial settings, B. licheniformis cells will be exposed to increases in the external osmolarity, conditions that trigger water efflux, impair turgor, cause the cessation of growth, and negatively affect the productivity of cell factories in biotechnological processes. We have taken here both systems-wide and targeted physiological approaches to unravel the core of the osmostress responses of B. licheniformis. Cells were suddenly subjected to an osmotic upshift of considerable magnitude (with 1 M NaCl), and their transcriptional profile was then recorded in a time-resolved fashion on a genome-wide scale. A bioinformatics cluster analysis was used to group the osmotically up-regulated genes into categories that are functionally associated with the synthesis and import of osmostress-relieving compounds (compatible solutes), the SigB-controlled general stress response, and genes whose functional annotation suggests that salt stress triggers secondary oxidative stress responses in B. licheniformis. The data set focusing on the transcriptional profile of B. licheniformis was enriched by proteomics aimed at identifying those proteins that were accumulated by the cells through increased biosynthesis in response to osmotic stress. Furthermore, these global approaches were augmented by a set of experiments that addressed the synthesis of the compatible solutes proline and glycine betaine and assessed the growth-enhancing effects of various osmoprotectants. Combined, our data provide a blueprint of the cellular adjustment processes of B. licheniformis to both sudden and sustained osmotic stress. Y1 - 2014 U6 - http://dx.doi.org/10.1371/journal.pone.0080956 SN - 1932-6203 VL - 8 IS - 11 PB - PLOS CY - San Francisco ER - TY - JOUR A1 - Handtke, Stefan A1 - Schroeter, Rebecca A1 - Jürgen, Britta A1 - Methling, Karen A1 - Schlüter, Rabea A1 - Albrecht, Dirk A1 - Hijum, Sacha A. F. T. van A1 - Bongaerts, Johannes A1 - Maurer, Karl-Heinz A1 - Lalk, Michael A1 - Schweder, Thomas A1 - Hecker, Michael A1 - Voigt, Birgit T1 - Bacillus pumilus reveals a remarkably high resistance to hydrogen peroxide provoked oxidative stress JF - PLOS one N2 - Bacillus pumilus is characterized by a higher oxidative stress resistance than other comparable industrially relevant Bacilli such as B. subtilis or B. licheniformis. In this study the response of B. pumilus to oxidative stress was investigated during a treatment with high concentrations of hydrogen peroxide at the proteome, transcriptome and metabolome level. Genes/proteins belonging to regulons, which are known to have important functions in the oxidative stress response of other organisms, were found to be upregulated, such as the Fur, Spx, SOS or CtsR regulon. Strikingly, parts of the fundamental PerR regulon responding to peroxide stress in B. subtilis are not encoded in the B. pumilus genome. Thus, B. pumilus misses the catalase KatA, the DNA-protection protein MrgA or the alkyl hydroperoxide reductase AhpCF. Data of this study suggests that the catalase KatX2 takes over the function of the missing KatA in the oxidative stress response of B. pumilus. The genome-wide expression analysis revealed an induction of bacillithiol (Cys-GlcN-malate, BSH) relevant genes. An analysis of the intracellular metabolites detected high intracellular levels of this protective metabolite, which indicates the importance of bacillithiol in the peroxide stress resistance of B. pumilus. Y1 - 2014 U6 - http://dx.doi.org/10.1371/journal.pone.0085625 SN - 1932-6203 VL - 9 IS - 1 PB - PLOS CY - San Francisco ER - TY - JOUR A1 - Immel, Timo A. A1 - Groth, Ulrich A1 - Huhn, Thomas A1 - Öhlschläger, Peter T1 - Titanium salan complexes displays strong antitumor properties in vitro and in vivo in mice JF - PLoS ONE N2 - The anticancer activity of titanium complexes has been known since the groundbreaking studies of Köpf and Köpf-Maier on titanocen dichloride. Unfortunately, possibly due to their fast hydrolysis, derivatives of titanocen dichloride failed in clinical studies. Recently, the new family of titanium salan complexes containing tetradentate ONNO ligands with anti-cancer properties has been discovered. These salan complexes are much more stabile in aqueous media. In this study we describe the biological activity of two titanium salan complexes in a mouse model of cervical cancer. High efficiency of this promising complex family was demonstrated for the first time in vivo. From these data we conclude that titanium salan complexes display very strong antitumor properties exhibiting only minor side effects. Our results may influence the chemotherapy with metallo therapeutics in the future. Y1 - 2011 U6 - http://dx.doi.org/10.1371/journal.pone.0017869 VL - 6 IS - 3 PB - Plos CY - San Francisco, California, US ER - TY - JOUR A1 - Berndt, Heinz T1 - Zur Reaktion von Iminodithiocarbonaten mit Carbonsäuren. I : Synthese des Modellpeptid-Derivates Z-(L)-Ala-(L/D)-Phe-(L)-Val-OMe JF - Tetrahedron letters Y1 - 1980 U6 - http://dx.doi.org/10.1016/S0040-4039(00)78663-1 SN - 0040-4039 VL - 21 IS - 34 SP - 3265 EP - 3268 ER -