TY  - CHAP
A1  - Welden, Melanie
A1  - Severins, Robin
A1  - Poghossian, Arshak
A1  - Wege, Christina
A1  - Siegert, Petra
A1  - Keusgen, Michael
A1  - Schöning, Michael Josef
T1  - Studying the immobilization of acetoin reductase with Tobacco mosaic virus particles on capacitive field-effect sensors
T2  - 2022 IEEE International Symposium on Olfaction and Electronic Nose (ISOEN)
N2  - A capacitive electrolyte-insulator-semiconductor (EISCAP) biosensor modified with Tobacco mosaic virus (TMV) particles for the detection of acetoin is presented. The enzyme acetoin reductase (AR) was immobilized on the surface of the EISCAP using TMV particles as nanoscaffolds. The study focused on the optimization of the TMV-assisted AR immobilization on the Ta 2 O 5 -gate EISCAP surface. The TMV-assisted acetoin EISCAPs were electrochemically characterized by means of leakage-current, capacitance-voltage, and constant-capacitance measurements. The TMV-modified transducer surface was studied via scanning electron microscopy.
KW  - Tobacco mosaic virus
KW  - acetoin
KW  - capacitive field-effect biosensor
KW  - enzyme immobilization
Y1  - 2022
SN  - 978-1-6654-5860-3 (Online)
SN  - 978-1-6654-5861-0 (Print)
U6  - http://dx.doi.org/10.1109/ISOEN54820.2022.9789657
N1  - IEEE International Symposium on Olfaction and Electronic Nose (ISOEN), 29 May 2022 - 01 June 2022, Aveiro, Portugal.
PB  - IEEE
ER  - 
TY  - JOUR
A1  - Molinnus, Denise
A1  - Janus, Kevin Alexander
A1  - Fang, Anyelina C.
A1  - Drinic, Aleksander
A1  - Achtsnicht, Stefan
A1  - Köpf, Marius
A1  - Keusgen, Michael
A1  - Schöning, Michael Josef
T1  - Thick-film carbon electrode deposited onto a biodegradable fibroin substrate for biosensing applications
JF  - Physica status solidi (a)
N2  - This study addresses a proof-of-concept experiment with a biocompatible screen-printed carbon electrode deposited onto a biocompatible and biodegradable substrate, which is made of fibroin, a protein derived from silk of the Bombyx mori silkworm. To demonstrate the sensor performance, the carbon electrode is functionalized as a glucose biosensor with the enzyme glucose oxidase and encapsulated with a silicone rubber to ensure biocompatibility of the contact wires. The carbon electrode is fabricated by means of thick-film technology including a curing step to solidify the carbon paste. The influence of the curing temperature and curing time on the electrode morphology is analyzed via scanning electron microscopy. The electrochemical characterization of the glucose biosensor is performed by amperometric/voltammetric measurements of different glucose concentrations in phosphate buffer. Herein, systematic studies at applied potentials from 500 to 1200 mV to the carbon working electrode (vs the Ag/AgCl reference electrode) allow to determine the optimal working potential. Additionally, the influence of the curing parameters on the glucose sensitivity is examined over a time period of up to 361 days. The sensor shows a negligible cross-sensitivity toward ascorbic acid, noradrenaline, and adrenaline. The developed biocompatible biosensor is highly promising for future in vivo and epidermal applications.
KW  - biocompatible materials
KW  - biodegradable electronic devices
KW  - biosensors
KW  - carbon electrodes
KW  - glucose
Y1  - 2022
U6  - http://dx.doi.org/10.1002/pssa.202200100
SN  - 1862-6319
N1  - Corresponding author: Michael J. Schöning
VL  - 219
IS  - 23
SP  - 1
EP  - 9
PB  - Wiley-VCH
CY  - Weinheim
ER  -