TY  - JOUR
A1  - Enning, Manfred
A1  - Schmidt, Bernd
A1  - Wilbring, Daniela
T1  - Auf dem Weg zur autonomen Anschlussbedienung
JF  - Privatbahn-Magazin
Y1  - 2019
SN  - 1865-0163
IS  - 3
SP  - 40
EP  - 43
PB  - Bahn Media
CY  - Suhlendorf
ER  - 
TY  - JOUR
A1  - Wingens, Niklas
A1  - May, Jörg
A1  - Pfaff, Raphael
T1  - Betrieblich-technische Innovationsansätze zur Bremsprobe nach einer Prozessänderung
JF  - ETR - Eisenbahntechnische Rundschau
Y1  - 2019
SN  - 0013-2845
IS  - 5
PB  - DVV Media Group
CY  - Hamburg
ER  - 
TY  - JOUR
A1  - Thomas, Axel
T1  - Die Wiederbelebung der Mitarbeiterwohnungsidee bei kommunalen Unternehmen
JF  - VM Verwaltung und Management
Y1  - 2019
U6  - https://doi.org/10.5771/0947-9856-2019-6-286
SN  - 0947-9856
VL  - 25
IS  - 6
SP  - 286
EP  - 291
PB  - Nomos-Verl.-Ges.
CY  - Baden-Baden
ER  - 
TY  - JOUR
A1  - Schwarz, Alexander
A1  - Gebhardt, Andreas
A1  - Schleser, Markus
A1  - Popoola, Patricia
T1  - New Welding Joint Geometries Manufactured by Powder Bed Fusion from 316L
JF  - Materials Performance and Characterization 8
Y1  - 2019
U6  - https://doi.org/10.1520/MPC20180096
SN  - 2379-1365
IS  - in press
ER  - 
TY  - JOUR
A1  - Kunkel, Maximilian Hugo
A1  - Gebhardt, Andreas
A1  - Mpofu, Khumbulani
A1  - Kallweit, Stephan
T1  - Quality assurance in metal powder bed fusion via deep-learning-based image classification
JF  - Rapid Prototyping Journal
Y1  - 2019
U6  - https://doi.org/10.1108/RPJ-03-2019-0066
SN  - 1355-2546
VL  - 26
IS  - 2
SP  - 259
EP  - 266
ER  - 
TY  - JOUR
A1  - Knödler, Matthias
A1  - Rühl, Clemens
A1  - Emonts, Jessica
A1  - Buyel, Johannes Felix
T1  - Seasonal weather changes affect the yield and quality of recombinant proteins produced in transgenic tobacco plants in a greenhouse setting
JF  - Frontiers in Plant Science
N2  - Transgenic plants have the potential to produce recombinant proteins on an agricultural scale, with yields of several tons per year. The cost-effectiveness of transgenic plants increases if simple cultivation facilities such as greenhouses can be used for production. In such a setting, we expressed a novel affinity ligand based on the fluorescent protein DsRed, which we used as a carrier for the linear epitope ELDKWA from the HIV-neutralizing antibody 2F5. The DsRed-2F5-epitope (DFE) fusion protein was produced in 12 consecutive batches of transgenic tobacco (Nicotiana tabacum) plants over the course of 2 years and was purified using a combination of blanching and immobilized metal-ion affinity chromatography (IMAC). The average purity after IMAC was 57 ± 26% (n = 24) in terms of total soluble protein, but the average yield of pure DFE (12 mg kg−1) showed substantial variation (± 97 mg kg−1, n = 24) which correlated with seasonal changes. Specifically, we found that temperature peaks (>28°C) and intense illuminance (>45 klx h−1) were associated with lower DFE yields after purification, reflecting the loss of the epitope-containing C-terminus in up to 90% of the product. Whereas the weather factors were of limited use to predict product yields of individual harvests conducted for each batch (spaced by 1 week), the average batch yields were well approximated by simple linear regression models using two independent variables for prediction (illuminance and plant age). Interestingly, accumulation levels determined by fluorescence analysis were not affected by weather conditions but positively correlated with plant age, suggesting that the product was still expressed at high levels, but the extreme conditions affected its stability, albeit still preserving the fluorophore function. The efficient production of intact recombinant proteins in plants may therefore require adequate climate control and shading in greenhouses or even cultivation in fully controlled indoor farms.
KW  - batch reproducibility
KW  - environmental correlation
KW  - fluorescent protein carrier
KW  - greenhouse cultivation
KW  - plant molecular farming
Y1  - 2019
U6  - https://doi.org/10.3389/fpls.2019.01245
SN  - 1664-462X  (online-ressource)
IS  - 10
PB  - Frontiers Media
CY  - Lausanne
ER  - 
TY  - JOUR
A1  - Wilbring, Daniela
A1  - Enning, Manfred
T1  - Stromversorgung auf Güterwagen - Aktuelle Bemühungen zur Standardisierung
JF  - ETR - Eisenbahntechnische Rundschau
Y1  - 2019
SN  - 0013-2845
VL  - 68
IS  - 11
SP  - 64
EP  - 67
PB  - DVV Media Group
CY  - Hamburg
ER  - 
TY  - JOUR
A1  - Panc, Nicolae
A1  - Contiu, Glad
A1  - Bocanet, Vlad
A1  - Thurn, Laura
A1  - Sabau, Emilia
T1  - The influence of cutting technology on surface wear hardness
JF  - Academic Journal of Manufacturing Engineering
Y1  - 2019
SN  - 1583-7904
VL  - 17
IS  - 3
SP  - 205
EP  - 210
ER  - 
TY  - JOUR
A1  - Bucur, Alexandru
A1  - Lazarescu, Lucian
A1  - Pop, Grigore Marian
A1  - Achimas, Gheorghe
A1  - Gebhardt, Andreas
T1  - Tribological performance of biodegradable lubricants under different surface roughness of tools
JF  - Academic Journal of Manufacturing Engineering
Y1  - 2019
SN  - 1583-7904
VL  - 17
IS  - 1
SP  - 172
EP  - 178
ER  -