TY - JOUR A1 - Rachinger, Michael A1 - Bauch, Melanie A1 - Strittmatter, Axel A1 - Bongaerts, Johannes A1 - Evers, Stefan A1 - Maurer, Karl-Heinz A1 - Daniel, Rolf A1 - Liebl, Wolfgang A1 - Liesegang, Heiko A1 - Ehrenreich, Armin T1 - Size unlimited markerless deletions by a transconjugative plasmid-system in Bacillus licheniformis JF - Journal of biotechnology Y1 - 2013 SN - 1873-4863 (E-Journal); 0168-1656 (Print) VL - Vol. 164 IS - Iss. 4 SP - 365 EP - 369 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Muschallik, Lukas A1 - Kipp, Carina Ronja A1 - Recker, Inga A1 - Bongaerts, Johannes A1 - Pohl, Martina A1 - Gelissen, Melanie A1 - Schöning, Michael Josef A1 - Selmer, Thorsten A1 - Siegert, Petra T1 - Synthesis of α-hydroxy ketones and vicinal diols with the Bacillus licheniformis DSM 13T butane-2, 3-diol dehydrogenase JF - Journal of Biotechnology N2 - The enantioselective synthesis of α-hydroxy ketones and vicinal diols is an intriguing field because of the broad applicability of these molecules. Although, butandiol dehydrogenases are known to play a key role in the production of 2,3-butandiol, their potential as biocatalysts is still not well studied. Here, we investigate the biocatalytic properties of the meso-butanediol dehydrogenase from Bacillus licheniformis DSM 13T (BlBDH). The encoding gene was cloned with an N-terminal StrepII-tag and recombinantly overexpressed in E. coli. BlBDH is highly active towards several non-physiological diketones and α-hydroxyketones with varying aliphatic chain lengths or even containing phenyl moieties. By adjusting the reaction parameters in biotransformations the formation of either the α-hydroxyketone intermediate or the diol can be controlled. Y1 - 2020 SN - 2590-1559 U6 - https://doi.org/10.1016/j.jbiotec.2020.09.016 VL - 202 IS - Vol. 324 SP - 61 EP - 70 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Abouzar, Maryam H. A1 - Poghossian, Arshak A1 - Pedraza, A. M. A1 - Gandhi, D. A1 - Ingebrandt, S. A1 - Moritz, W. A1 - Schöning, Michael Josef T1 - An array of field-effect nanoplate SOI capacitors for (bio-)chemical sensing JF - Biosensors and Bioelectronics. 26 (2011), H. 6 Y1 - 2011 SN - 0956-5663 SP - 3023 EP - 3028 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Wagner, Torsten A1 - Vornholt, Wolfgang A1 - Werner, Frederik A1 - Yoshinobu, Tatsuo A1 - Miyamoto, Ko-Ichiro A1 - Keusgen, Michael A1 - Schöning, Michael Josef T1 - Light-addressable potentiometric sensor (LAPS) combined with magnetic beads for pharmaceutical screening JF - Physics in medicine N2 - The light-addressable potentiometric sensor (LAPS) has the unique feature to address different regions of a sensor surface without the need of complex structures. Measurements at different locations on the sensor surface can be performed in a common analyte solution, which distinctly simplifies the fluidic set-up. However, the measurement in a single analyte chamber prevents the application of different drugs or different concentrations of a drug to each measurement spot at the same time as in the case of multi-reservoir-based set-ups. In this work, the authors designed a LAPS-based set-up for cell culture screening that utilises magnetic beads loaded with the endotoxin (lipopolysaccharides, LPS), to generate a spatially distributed gradient of analyte concentration. Different external magnetic fields can be adjusted to move the magnetic beads loaded with a specific drug within the measurement cell. By recording the metabolic activities of a cell layer cultured on top of the LAPS surface, this work shows the possibility to apply different concentrations of a sample along the LAPS measurement spots within a common analyte solution. Y1 - 2016 U6 - https://doi.org/10.1016/j.phmed.2016.03.001 SN - 2352-4510 VL - 2016 IS - 1 SP - 2 EP - 7 ER - TY - JOUR A1 - Breuer, Lars A1 - Pilas, Johanna A1 - Guthmann, Eric A1 - Schöning, Michael Josef A1 - Thoelen, Ronald A1 - Wagner, Torsten T1 - Towards light-addressable flow control: responsive hydrogels with incorporated graphene oxide as laser-driven actuator structures within microfluidic channels JF - Sensor and Actuators B: Chemical Y1 - 2019 U6 - https://doi.org/10.1016/j.snb.2019.02.086 SN - 0925-4005 VL - 288 SP - 579 EP - 585 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Cornelis, Peter A1 - Givanoudi, Stella A1 - Yongabi, Derick A1 - Iken, Heiko A1 - Duwé, Sam A1 - Deschaume, Olivier A1 - Robbens, Johan A1 - Dedecker, Peter A1 - Bartic, Carmen A1 - Wübbenhorst, Michael A1 - Schöning, Michael Josef A1 - Heyndrickx, Marc A1 - Wagner, Patrick T1 - Sensitive and specific detection of E. coli using biomimetic receptors in combination with a modified heat-transfer method JF - Biosensors and Bioelectronics Y1 - 2019 U6 - https://doi.org/10.1016/j.bios.2019.04.026 SN - 0956-5663 VL - 136 SP - 97 EP - 105 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Arreola, Julio A1 - Oberländer, Jan A1 - Mätzkow, M. A1 - Keusgen, Michael A1 - Schöning, Michael Josef T1 - Surface functionalization for spore-based biosensors with organosilanes JF - Electrochimica Acta N2 - In the present work, surface functionalization of different sensor materials was studied. Organosilanes are well known to serve as coupling agent for biomolecules or cells on inorganic materials. 3-aminopropyltriethoxysilane (APTES) was used to attach microbiological spores time to an interdigitated sensor surface. The functionality and physical properties of APTES were studied on isolated sensor materials, namely silicon dioxide (SiO2) and platinum (Pt) as well as the combined material on sensor level. A predominant immobilization of spores could be demonstrated on SiO2 surfaces. Additionally, the impedance signal of APTES-functionalized biosensor chips has been investigated. Y1 - 2017 U6 - https://doi.org/10.1016/j.electacta.2017.04.157 SN - 0013-4686 VL - 241 SP - 237 EP - 243 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Oberländer, Jan A1 - Mayer, Marlena A1 - Greeff, Anton A1 - Keusgen, Michael A1 - Schöning, Michael Josef T1 - Spore-based biosensor to monitor the microbicidal efficacy of gaseous hydrogen peroxide sterilization processes JF - Biosensors and Bioelectronics N2 - In this work, a spore-based biosensor is evaluated to monitor the microbicidal efficacy of sterilization processes applying gaseous hydrogen peroxide (H2O2). The sensor is based on interdigitated electrode structures (IDEs) that have been fabricated by means of thin-film technologies. Impedimetric measurements are applied to study the effect of sterilization process on spores of Bacillus atrophaeus. This resilient microorganism is commonly used in industry to proof the sterilization efficiency. The sensor measurements are accompanied by conventional microbiological challenge tests, as well as morphological characterizations with scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The sensor measurements are correlated with the microbiological test routines. In both methods, namely the sensor-based and microbiological one, a tailing effect has been observed. The results are evaluated and discussed in a three-dimensional calibration plot demonstrating the sensor's suitability to enable a rapid process decision in terms of a successfully performed sterilization. Y1 - 2018 U6 - https://doi.org/10.1016/j.bios.2017.12.045 SN - 0956-5663 VL - 104 SP - 87 EP - 94 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Arreola, Julio A1 - Keusgen, Michael A1 - Schöning, Michael Josef T1 - Toward an immobilization method for spore-based biosensors in oxidative environment JF - Electrochimica Acta Y1 - 2019 U6 - https://doi.org/10.1016/j.electacta.2019.01.148 VL - 302 SP - 394 EP - 401 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Arreola, Julio A1 - Keusgen, Michael A1 - Wagner, Torsten A1 - Schöning, Michael Josef T1 - Combined calorimetric gas- and spore-based biosensor array for online monitoring and sterility assurance of gaseous hydrogen peroxide in aseptic filling machines JF - Biosensors and Bioelectronics Y1 - 2019 U6 - https://doi.org/10.1016/j.bios.2019.111628 SN - 0956-5663 VL - 143 IS - 111628 PB - Elsevier CY - Amsterdam ER -