TY - JOUR A1 - Beging, Stefan A1 - Leinhos, Marcel A1 - Jablonski, Melanie A1 - Poghossian, Arshak A1 - Schöning, Michael Josef T1 - Studying the spatially resolved immobilisation of enzymes on a capacitive field-effect structure by means of nano-spotting JF - Physica status solidi (a) Y1 - 2015 U6 - https://doi.org/10.1002/pssa.201431891 SN - 1862-6319 VL - 212 IS - 6 SP - 1353 EP - 1358 PB - Wiley CY - Weinheim ER - TY - JOUR A1 - Digel, Ilya A1 - Wehlitz, V. A1 - Kayser, Peter A1 - Figiel-Lange, A. A1 - Bassam, R. A1 - Rundstedt, F. von T1 - Suspension depletion approach for exemption of infected Solanum jasminoides cells from pospiviroids JF - Plant Pathology N2 - Despite numerous studies, viroid elimination from infected plants remains a very challenging task. This study introduces for the first time a novel ‘suspension depletion’ approach for exemption of Solanum jasminoides plants from viroids. The proposed method implies initial establishment of suspension cultures of the infected plant cells. The suspended cells were then physically treated (mild thermotherapy, 33 °C), which presumably delayed the replication of the viroid. The viroid concentration in the treated biomass was monitored weekly using pospiviroid-specific PCR. After 10–12 weeks of continuous treatment, a sufficient decrease in viroid concentration was observed such that the infection became undetectable by PCR. The treated single cells then gave rise to microcolonies on a solid culture medium and the obtained viroid-negative clones were further promoted to regenerate into viroid-free plants. Three years of accumulated experimental data suggests feasibility, broad applicability, and good efficacy of the proposed approach. Y1 - 2018 U6 - https://doi.org/10.1111/ppa.12750 SN - 1365-3059 VL - 67 IS - 2 SP - 358 EP - 365 PB - Wiley CY - Oxford ER - TY - JOUR A1 - Heinze, Daniel A1 - Mang, Thomas A1 - Peter, Karin A1 - Möller, Martin A1 - Weichold, Oliver T1 - Synthesis of low molecular weight poly(vinyl acetate) and its application as plasticizer JF - Journal of applied polymer science N2 - Poly(vinyl acetate), PVAc, with a degree of polymerization Xn = 10 was prepared by chain-transfer radical polymerization using carbon tetrachloride and used as oligomeric plasticizer for commercial PVAc. However, the chlorinated chain ends cause a low thermal stability requiring mild Cl/H substitution. The product exhibits high thermal stability and excellent melt-compounding properties. Blends of oligomeric and commercial PVAc show single glass transition temperatures which decrease with higher oligomer content and exhibit small negative deviations from Fox' linear additivity rule. This indicates plasticization and miscibility being mainly due to entropic effects. Injection-moulded thick specimens show ductile behaviour at oligomer contents >10 wt %, while sheets with a thickness of 0.2–0.5 mm appear flexible already at 7.5 wt %. The oxygen permeability coefficients are an order of magnitude lower than those of low-density polyethylene. Due to the sum of their properties, the plasticized sheets present a promising alternative in the preparation of barrier materials. Y1 - 2014 U6 - https://doi.org/10.1002/app.40226 SN - 1097-4628 (E-Journal); 0021-8995 (Print) VL - 131 IS - 9 SP - Article No. 40226 PB - Wiley CY - New York ER - TY - JOUR A1 - Öhlschläger, Peter A1 - Spies, Elmar A1 - Alvarez, Gerardo A1 - Quetting, Michael A1 - Groettrup, Marcus T1 - The combination of TLR-9 adjuvantation and electroporation-mediated delivery enhances in vivo antitumor responses after vaccination with HPV-16 E7 encoding DNA JF - International Journal of Cancer. 128 (2011), H. 2 Y1 - 2011 SN - 1097-0215 SP - 473 EP - 481 PB - Wiley CY - Weinheim ER - TY - JOUR A1 - Lyons, W. Berry A1 - Mikucki, Jill A. A1 - German, Laura A. A1 - Welch, Kathleen A. A1 - Welch, Susan A. A1 - Gardener, Christopher B. A1 - Tulaczyk, Slawek M. A1 - Pettit, Erin C. A1 - Kowalski, Julia A1 - Dachwald, Bernd T1 - The Geochemistry of Englacial Brine from Taylor Glacier, Antarctica JF - Journal of Geophysical Research: Biogeosciences Y1 - 2019 U6 - https://doi.org/10.1029/2018JG004411 SN - 2169-8961 PB - Wiley CY - Hoboken ER - TY - JOUR A1 - Voigt, Birgit A1 - Schroeter, Rebecca A1 - Jürgen, Britta A1 - Albrecht, Dirk A1 - Evers, Stefan A1 - Bongaerts, Johannes A1 - Maurer, Karl-Heinz A1 - Schweder, Thomas A1 - Hecker, Michael T1 - The response of Bacillus licheniformis to heat and ethanol stress and the role of the SigB regulon JF - Proteomics Y1 - 2013 SN - 1615-9861 (E-Journal); 1615-9853 (Print) VL - Vol. 13 IS - Iss. 14 SP - 2140 EP - 2146 PB - Wiley CY - Weinheim ER - TY - JOUR A1 - Hugenroth, Kristin A1 - Neidlin, Michael A1 - Engelmann, Ulrich M. A1 - Kaufmann, Tim A. S. A1 - Steinseifer, Ulrich A1 - Heilmann, Torsten T1 - Tipless Transseptal Cannula Concept Combines Improved Hemodynamic Properties and Risk‐Reduced Placement: an In Silico Proof‐of‐Concept JF - Artificial Organs Y1 - 2021 U6 - https://doi.org/10.1111/aor.13964 SN - 1525-1594 IS - Accepted Article PB - Wiley CY - Weinheim ER - TY - JOUR A1 - Oberländer, Jan A1 - Bromm, Alexander A1 - Wendeler, Luisa A1 - Iken, Heiko A1 - Palomar Duran, Marlena A1 - Greeff, Anton A1 - Kirchner, Patrick A1 - Keusgen, Michael A1 - Schöning, Michael Josef T1 - Towards a biosensor to monitor the sterilisation efficiency of aseptic filling machines JF - Physica status solidi (a) N2 - Sterilisation processes are compulsory in medicine, pharmacy, and food industries to prevent infections of consumers and microbiological contaminations of products. Monitoring the sterilisation by conventional microbiological methods is time- and lab-consuming. To overcome this problem, in this work a novel biosensor has been proposed. The sensor enables a fast method to evaluate sterilisation processes. By means of thin-film technology the sensor's transducer structures in form of IDEs (interdigitated electrodes) have been fabricated on a silicon substrate. Physical characterisation of the developed sensor was done by AFM, SEM, and profilometry. Impedance analyses were conducted for the electrical characterisation. As microbiological layer spores of B. atrophaeus have been immobilised on the sensing structure; spores of this type are a well-known sterilisation test organism. Impedance measurements at a fixed frequency over time were performed to monitor the immobilisation process. A sterilisation process according to aseptic filling machines was applied to demonstrate the sensor functionality. After both, immobilisation and sterilisation, a change in impedance could successfully be detected. Y1 - 2015 U6 - https://doi.org/10.1002/pssa.201431900 SN - 1862-6319 VL - 212 IS - 6 SP - 1299 EP - 1305 PB - Wiley CY - Weinheim ER - TY - JOUR A1 - Reisert, Steffen A1 - Geissler, Hanno A1 - Flörke, Rudolf A1 - Näther, Niko A1 - Wagner, Patrick A1 - Schöning, Michael Josef T1 - Towards a multi-sensor system for the evaluation of aseptic processes employing hydrogen peroxide vapour (H2O2) JF - Physica status solidi (a) : applications and material science. 208 (2011), H. 6 Y1 - 2011 SN - 1862-6319 SP - 1351 EP - 1356 PB - Wiley CY - Weinheim ER - TY - JOUR A1 - Kirchner, Patrick A1 - Oberländer, Jan A1 - Suso, Henri-Pierre A1 - Rysstad, Gunnar A1 - Keusgen, Michael A1 - Schöning, Michael Josef T1 - Towards a wireless sensor system for real-time H2O2 monitoring in aseptic food processes JF - Physica status solidi (a) N2 - A wireless sensor system based on the industrial ZigBee standard for low-rate wireless networking was developed that enables real-time monitoring of gaseous H2O2 during the package sterilization in aseptic food processes. The sensor system consists of a remote unit connected to a calorimetric gas sensor, which was already established in former works, and an external base unit connected to a laptop computer. The remote unit was built up by an XBee radio frequency (RF) module for data communication and a programmable system-on-chip controller to read out the sensor signal and process the sensor data, whereas the base unit is a second XBee RF module. For the rapid H2O2 detection on various locations inside the package that has to be sterilized, a novel read-out strategy of the calorimetric gas sensor was established, wherein the sensor response is measured within the short sterilization time and correlated with the present H2O2 concentration. In an exemplary measurement application in an aseptic filling machinery, the suitability of the new, wireless sensor system was demonstrated, wherein the influence of the gas velocity on the H2O2 distribution inside a package was determined and verified with microbiological tests. KW - calorimetric gas sensor;hydrogen peroxide;wireless sensor system Y1 - 2013 U6 - https://doi.org/10.1002/pssa.201200920 SN - 1862-6319 VL - 210 IS - 5 SP - 877 EP - 883 PB - Wiley CY - Weinheim ER -